BACKGROUDWe previously demonstrated that Shaziling and Yorkshire pigs differ in growth rate and meat quality. However, the molecular mechanisms responsible for such phenotypic differences remains unclear. Here, we performed a transcriptomic analysis of 36 longissimus dorsi (LM) and 36 soleus (SM) muscle samples from Shaziling and Yorkshire pigs at six postnatal stages (30, 60, 90, 150, 210, and 300 days) to explore the differences in postnatal skeletal muscle of Shaziling and Yorkshire pigs.ResultsMuscle morphological changes and the number of differentially expressed genes (DEGs) indicated the two stages of 60 to 90 d and 150 to 210 d were critical for the muscle growth and development in Shaziling pigs. The genes such as FLNC, COL1A1, NRAP, SMYD1, TNNI3, CRYAB, and PDLIM3 played vital roles in the muscle growth, and the genes such as CCDC71L, LPIN1, CPT1A, UCP3, NR4A3, and PDK4 played dominant roles in the lipid metabolism. Additionally, in contrast to the LM, the percentage of slow‐twitch muscle fibers in the SM of both breeds consistently decreased from 30 to 150 days of age, but there was a significant rebound at 210 days of age. However, the percentage of slow‐twitch muscle fibers in the SM of Shaziling pigs was higher than that in Yorkshire pigs, which may be associated with the calcium signaling pathway and PPARβ/δ signaling pathway.ConclusionThis study detected two critical periods and many functional genes for the muscle growth and development of Shaziling pigs, and showed differences in muscle fiber characteristics between Shaziling and Yorkshire pigs.This article is protected by copyright. All rights reserved.

中文翻译：

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使用 RNA-Seq 分析沙子岭猪和约克夏猪骨骼肌生长和发育的动态转录组图谱

背景我们之前已经证明沙子岭猪和约克夏猪在生长速度和肉质方面存在差异。然而，造成这种表型差异的分子机制仍不清楚。在这里，我们对 36 个背最长肌（LM）和 36比目鱼（SM）采集沙子岭猪和约克夏猪产后6个阶段（30、60、90、150、210和300天）的肌肉样本，探讨沙子岭和约克夏猪产后骨骼肌的差异。结果肌肉形态变化和数量差异表达基因(DEG)表明60~90天和150~210天两个阶段对于沙子岭猪的肌肉生长发育至关重要。基因如FLNC，COL1A1,国家研究计划,SMYD1,TNNI3,克瑞亚布， 和PDLIM3在肌肉生长中发挥着至关重要的作用，诸如CDC71L,LPIN1,CPT1A,UCP3,NR4A3， 和PDK4在脂质代谢中起主导作用。此外，与 LM 相比，两个品种的 SM 中慢肌纤维的百分比从 30 日龄到 150 日龄持续下降，但在 210 日龄时出现显着反弹。但沙子岭猪的SM中慢肌纤维的比例高于约克夏猪，这可能与钙信号通路和PPARβ/δ信号通路有关。结论本研究检测到了两个关键时期和许多功能基因对沙子岭猪的肌肉生长发育进行了研究，并显示了沙子岭猪和约克夏猪之间肌肉纤维特征的差异。本文受版权保护。版权所有。