ObjectivesFemale obesity may be improved by the consumption of a high-polyphenolic-rich diet. Lycium ruthenicum Murray is a renowned edible plant, the fruit of which is abundant in anthocyanins and exhibits many biological activities. This study aimed to investigate the potential impact of anthocyanins derived from Lycium ruthenicum Murray (ACN) intervention on improving obesity by regulating the gut microbiota and bile acids (BAs) metabolism in high-fat diet (HFD)-induced female mice.MethodsA total of 32 C57BL/6J female mice were divided into four distinct groups: the Ctrl group (fed a normal diet), Ctrl + ACN group (fed a normal diet plus 8 mg/mL extract of ACN), HFD group (fed a high-fat diet), and HFD + ACN group (fed a HFD plus 8 mg/mL extract of ACN).ResultsThe findings showed that ACN significantly reduced the body weight, periovarian adipose mass, and adipocyte diameter, ameliorated lipid accumulation in the liver, and lowered the serum total cholesterol and low-density lipoprotein (LDL-C) levels in HFD-induced female mice. In addition, ACN exhibited a reversal of gut microbial dysbiosis in HFD-fed female mice, such as by enhancing the quantity of Lactobacillus and Allobaculum and reducing the abundance of Blautia and Faecalibaculum. Moreover, the results of fecal BAs showed that ACN led to a decrease in the ratio of primary to secondary BAs, mainly attributed to decreased levels of primary BAs, including CA, CDCA, αMCA, and HCA in HFD-induced female mice. Further analysis revealed that ACN may exert its anti-obesity effect by increasing the relative abundance of Lactobacillus_ jonsonii and Lactobacillus_reuteri within the gut and subsequently affecting the metabolism of fecal HDCA and GUDCA.ConclusionThese results indicated that ACN effectively inhibits HFD-induced obesity in female mice by regulating gut microbiota-related BA metabolism.

中文翻译：

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黑果枸杞中的花青素通过肠道微生物相关的胆汁酸代谢预防雌性小鼠高脂饮食引起的肥胖

目的 食用富含多酚的饮食可以改善女性肥胖症。黑果枸杞是著名的食用植物，其果实富含花青素，具有多种生物活性。本研究旨在探讨黑果枸杞花青素 (ACN) 通过调节高脂饮食 (HFD) 诱导的雌性小鼠肠道微生物群和胆汁酸 (BA) 代谢来干预改善肥胖的潜在影响。将 32 只 C57BL/6J 雌性小鼠分为四个不同组：Ctrl 组（正常饮食喂养）、Ctrl + ACN 组（正常饮食加 8 mg/mL ACN 提取物喂养）、HFD 组（高脂饮食喂养）饮食）和 HFD + ACN 组（喂食 HFD 加 8 mg/mL ACN 提取物）。 结果 结果发现，ACN 显着降低了体重、卵巢周围脂肪量和脂肪细胞直径，改善了肝脏中的脂质积累，并降低了脂肪细胞直径。 HFD 诱导的雌性小鼠的血清总胆固醇和低密度脂蛋白 (LDL-C) 水平。此外，ACN 还可以逆转 HFD 喂养的雌性小鼠肠道微生物失调，例如通过增加乳杆菌和异菌的数量并减少 Blautia 和 Faecalibaculum 的丰度。此外，粪便BAs结果显示，ACN导致初级BAs与次级BAs的比例下降，这主要归因于HFD诱导的雌性小鼠初级BAs水平下降，包括CA、CDCA、αMCA和HCA。进一步分析发现，ACN可能通过增加肠道内Lactobacillus_jonsonii和Lactobacillus_reuteri的相对丰度，进而影响粪便HDCA和GUDCA的代谢来发挥其抗肥胖作用。结论：ACN有效抑制HFD诱导的雌性小鼠肥胖。通过调节肠道微生物群相关的 BA 代谢。