Coupling of biotechnology and mineral processing arise from the depletion of high-grade ores. () is a chemolithotroph, γ-proteobacterium, gram negative bacteria that has been used in biomining over the years due to its ability of bio mobilising valuable minerals and metals. In this study, the ability of ability to modify and pre-concentrate base metal sulphides from Upper Group 2 (UG2) was investigated. Mesophile (37 °C) and thermophile (47 °C) mixtures were cultured and used to condition UG2 run of mine sample for 4 h at a pH ranging from 7,5 to 7. The adherence of the bacteria to the UG2 run of mine (ROM) was confirmed with FTIR, XRD, XRF and XRD. Biomodification results suggested that Culture A significantly concentrated Cu, however Ni and S were not concentrated as desired, culture B showed the same effect at 4 h. Comparing the effect of culture, A to B, it was ruled that culture A had a high concentrating effect towards valuables (Cu, Ni, S and Fe which could be from sulphides or chromite) whereas culture B had a concentrating effect on the gangue elements such as Al, Si, Ca, Mg, and Cr. To study the effect of the bacteria on biomodification and surface oxidation, flotation was conducted. Cu grade and recovery obtained was 1.9 % and 87.33 % for mesophile and 1.44 % and 84.4 % for thermophile , respectively. Whereas for Ni, thermophile a higher grade of 1.35 % compared to 1.22 % obtained when mesophile was used. It was concluded that mesophile and thermophile could be used for the biomodification of chalcopyrite and pentlandite. But where selective flotation of chalcopyrite is desired, using mesophile would provide an effective process, whereas thermophile would provide optimum grade and recoveries for pentlandite. Nonetheless, to further optimize the bioflotation process, controlling conditioning time and pulp density was crucial for both mesophile -bioflotation and thermophile -bioflotation these two factors were ruled as the most factors that majorly influence the grade and recoveries of Cu, Ni.

中文翻译：

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使用氧化亚铁硫杆菌作为改性剂优化上组 2 浮选

生物技术和矿物加工的结合源于高品位矿石的枯竭。 () 是一种化能营养菌、γ-变形菌、革兰氏阴性细菌，由于其生物动员有价值的矿物质和金属的能力，多年来一直用于生物采矿。在本研究中，研究了对上族 2 (UG2) 贱金属硫化物进行改性和预浓缩的能力。培养嗜温菌 (37 °C) 和嗜热菌 (47 °C) 混合物，并将其用于在 7.5 至 7 的 pH 范围内对矿样的 UG2 运行进行调节 4 小时。细菌对矿样的 UG2 运行的粘附(ROM) 通过 FTIR、XRD、XRF 和 XRD 进行了确认。生物修饰结果表明，培养物 A 显着浓缩了 Cu，但 Ni 和 S 未达到预期浓缩，培养物 B 在 4 小时时显示出相同的效果。比较培养物A和B的效果，可以看出，培养物A对贵重元素（可能来自硫化物或铬铁矿的Cu、Ni、S和Fe）具有较高的富集作用，而培养物B对脉石元素具有较高的富集作用例如Al、Si、Ca、Mg、Cr等。为了研究细菌对生物改性和表面氧化的影响，进行了浮选。中温介质的铜品位和回收率分别为 1.9% 和 87.33%，嗜热介质的铜品位和回收率分别为 1.44% 和 84.4%。而对于 Ni，嗜热介质的品位更高，为 1.35 %，而使用嗜温介质时获得的镍品位为 1.22 %。结果表明，嗜温和嗜热介质可用于黄铜矿和镍黄铁矿的生物改性。但是，当需要选择性浮选黄铜矿时，使用中温介质将提供有效的工艺，而嗜热介质将为镍黄铁矿提供最佳品位和回收率。尽管如此，为了进一步优化生物浮选工艺，控制调节时间和矿浆密度对于中温生物浮选和嗜热生物浮选至关重要，这两个因素被认为是影响铜、镍品位和回收率的最主要因素。