Kisspeptin (Kiss) and kisspeptin receptor (Kissr) system is a key regulator of GnRH expression in several vertebrates. The Indian catfish, Clarias magur, is popular in the Indian sub-continent, and a neo-type of the Asian catfish, C. batrachus. Catfish breeding is constrained as males do not release milt captivity with/without stimulation. Magur Kiss/Kissr system comprising of kiss1, kiss2, kissr1, and kissr2 genes was characterized for the first time. Full-length mRNA was sequenced using RACE PCR. Neighbor-joining tree of predicted proteins shows one clade of teleost orthologs. Magur whole genome (NCBI GenBank) has single copies of each gene, though yet unannotated/misannotated. Anomalies in the nomenclature of earlier sequences in GenBank were noted. Relative gene expression was profiled during various ontogenic stages, in six tissues including brain and gonads at maturity, and also in brains and gonads of premature and spent fish. Expression of gnrh1, gnrhr1, and gnrhr2 was estimated concomitantly. The kiss1 was the first to be twofold upregulated (P < 0.05) at 12 h post fertilization. Kiss/Kissr genes expressed primarily in the brain, ovary, and testis. Though kiss2 was 10 times higher than kiss1, only kiss1 showed significant modulation across stages and appears to be the active isotype that regulates GnRH in magur.

Kisspeptin (Kiss) 和 Kisspeptin 受体 (Kissr) 系统是多种脊椎动物中 GnRH 表达的关键调节因子。印度鲶鱼（Clarias magur）在印度次大陆很受欢迎，而亚洲鲶鱼的新类型是C. batrachus。鲶鱼的繁殖受到限制，因为雄性鲶鱼在有/没有刺激的情况下都不会释放鱼白。首次对由Kiss1、Kiss2、Kissr1和Kissr2基因组成的 Magur Kiss/Kissr 系统进行了表征。使用 RACE PCR 对全长 mRNA 进行测序。预测蛋白质的邻接树显示了硬骨鱼直向同源物的一个分支。 Magur 全基因组（NCBI GenBank）每个基因都有单个拷贝，但尚未注释/错误注释。注意到 GenBank 中早期序列命名的异常。对不同个体发育阶段的相对基因表达进行了分析，包括成熟时的大脑和性腺以及早熟鱼和废鱼的大脑和性腺在内的六种组织。同时估计gnrh1、gnrhr1和gnrhr2的表达。 Kiss1是第一个 在受精后12小时双倍上调的（P <0.05）。 Kiss/Kissr 基因主要在大脑、卵巢和睾丸中表达。尽管Kiss2比Kiss1高 10 倍，但只有Kiss1显示出跨阶段的显着调节，并且似乎是调节 Magur 中 GnRH 的活性同种型。