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CORRIGENDUM
Molecular Ecology ( IF 4.9 ) Pub Date : 2024-04-22 , DOI: 10.1111/mec.17325


The authors wish to note an error in the article entitled “Epigenetics and island-mainland divergence in an insectivorous small mammal” published in Molecular Ecology (2023), 32(1), 152–166, https://doi.org/10.1111/mec.16735.

Upon evaluation of the mitochondrial cytochrome B sequence data and phenotypic measurements, we discovered that six individuals in our dataset were Sorex fumeus and not S. cinereus. This included the sample used for the reference assembly and those in the mainland comparison, reducing the number of mainland individuals to n = 6. This resulted in a total of 39 instead of 47 tissue samples used for the epigenetic clock, and 36 instead of 44 tissue samples used for the EWAS analyses. The reference assembly was only used to identify Sorex aligned probes from the mammal array and is common practice (e.g. Larison et al., 2021; Mayne et al., 2022)—this does not effect the analysis. The misidentified samples were removed from the dataset and all analyses were re-run. All the patterns from the original published paper remained unchanged. Updated tables and figures showing side-by-side comparisons are shown below. The supplemental figures and tables were also updated. We have corrected the sample record on NCBI (https://www.ncbi.nlm.nih.gov/nuccore/JAOANZ000000000.1/). The updated manuscript is included as supporting information for easy reference.

Table1 (original version):

TABLE 1. Trapping location of samples, N = total number of samples, number of females, minimum and maximum age. Age is represented as the estimated age in months divided by 12. Note, age of 0.00 represents fetus.
Location Island N No. of females Age (min/max)
Bon Portage Island, NS Yes 16 6 0.167/1.33
Long Island, NS Yes 9 4 0.00/1.25
Sandy Cove, NS No 4 3 0.250/0.375
North Mountain, NS No 2 1 0.417/0.417
Peterborough County, ON No 6 1 0.167/1.08

Table1 (corrected version):

TABLE 1. Trapping location of samples, N = total number of samples, number of females, minimum and maximum age. Age is represented as the estimated age in months divided by 12. Note, age of 0.00 represents fetus. *S. fumeus samples removed.
Location Island N No. of females Age (min/max)
Bon Portage Island, NS Yes 16 6 0.167/1.33
Long Island, NS Yes 9 4 0.00/1.25
Sandy Cove, NS No 4 3 0.250/0.375
North Mountain, NS No 2 1 0.417/0.417

Table2 (original version):

TABLE 2. Summary statistics for the linear regression models for weight, body length and skull length of mainland versus island masked shrews. Sex values are relative to females and location relative to mainland populations. The β-coefficient (estimate), 95% confidence interval (CI), p value and adjusted R2 are provided.
Estimate 95% CI P value Adj. R2
Model 1: Weight ~ Age + Sex + Location
Age 1.15 0.21 to 2.08 0.018 0.53
Sex 0.01 –0.61 to 0.64 0.962
Location –2.09 –2.79 to –1.39 <0.001
Model 2: Body length ~ Age + Sex + Location
Age 8.43 3.36 to 13.50 0.002 0.49
Sex –0.20 –3.61 to 3.20 0.903
Location –10.35 –14.15 to –6.54 <0.001
Model 3: Skull length ~ Age + Sex + Location
Age –0.31 –0.93 to 0.32 0.321 0.79
Sex –0.21 –0.63 to 0.21 0.319
Location –1.92 –2.39 to –1.45 <0.001

Table2 (corrected version):

TABLE 2. Summary statistics for the linear regression models for weight, body length and skull length of mainland versus island masked shrews. Sex values are relative to females and location relative to mainland populations. The β-coefficient (estimate), 95% confidence interval (CI), p value and adjusted R2 are provided. *S. fumeus samples removed.
Estimate 95% CI P value Adj. R2
Model 1: Weight ~ Age + Sex + Location
Age 0.96 –0.03 to 1.95 0.056 0.27
Sex –0.21 –0.89 to 0.47 0.535
Location –1.42 –2.26 to –0.57 0.002
Model 2: Body length ~ Age + Sex + Location
Age 6.95 1.27 to 12.63 0.019 0.33
Sex –0.30 –4.22 to 3.63 0.877
Location –8.80 –13.64 to –3.96 0.001
Model 3: Skull length ~ Age + Sex + Location
Age –0.38 –1.11 to 0.36 0.298 0.70
Sex –0.35 –0.86 to 0.16 0.170
Location –1.64 –2.27 to –1.01 <0.001

Table3 (original version):

TABLE 3. Top 5 genes near outlier CpGs for island versus mainland EWAS and BPI versus all other populations. Functions of each gene are abbreviated from Uniprot.
Gene name CpG Direction Location (p) Putative function
Island-mainland
EWS exon cg11703808 pos 3.35e-21 Transcriptional repressor and might play a role in the tumorigenic process.
PO4F3 intron cg00152260 pos 1.23e-19 Transcriptional activator. Involved in the auditory system development.
UBP54 intron cg22063749 neg 3.27e-19 Has no peptidase activity.
TRI47 exon cg25115601 neg 3.12e-16 Mediates the ubiquitination and proteasomal degradation of CYLD.
ATPA exon cg24102071 neg 6.37e-16 Produces ATP from ADP.
BPI
SMO exon cg20625795 neg 1.03e-30 Associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal.
ZO1 exon cg03673751 neg 2.00e-21 Related to movement of substances through the paracellular space. Plays a role in the regulation of cell migration.
PRP8 promoter cg25476805 neg 7.60e-19 Plays role in pre-mRNA splicing.
CBX5 intergenic cg23036219 neg 1.12e-18 Associated with epigenetic repression. Involved in the formation of functional kinetochore.
ZBT20 intergenic cg00903722 neg 3.32e-17 Transcription factor that may be involved in hematopoiesis, oncogenesis, and immune responses. Plays a role in postnatal myogenesis.

Table3 (corrected version):

TABLE 3. Top 5 genes near outlier CpGs for island versus mainland EWAS and BPI versus all other populations. Functions of each gene are abbreviated from Uniprot. *S. fumeus samples removed.
Gene name CpG Direction Location (p) Putative function
Island-mainland
EWS exon cg11703808 pos 5.76e-18 Transcriptional repressor and might play a role in the tumorigenic process.
PO4F3 intron cg00152260 pos 7.32e-14 Transcriptional activator. Involved in the auditory system development.
UBP54 intron cg22063749 neg 1.71e-13 Has no peptidase activity.
MYH6 exon cg11736686 pos 5.48e-12 Actin-based motor molecules with ATPase activity.
TRI47 exon cg25115601 neg 2.07e-11 Mediates the ubiquitination and proteasomal degradation of CYLD.
BPI
SMO exon cg20625795 neg 1.06e-24 Associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal.
ZO1 exon cg03673751 neg 6.04e-16 Related to movement of substances through the paracellular space. Plays a role in the regulation of cell migration.
PRP8 promoter cg25476805 neg 2.57e-14 Plays role in pre-mRNA splicing.
CBX5 intergenic cg23036219 neg 1.10e-13 Associated with epigenetic repression. Involved in the formation of functional kinetochore.
ZBT20 intergenic cg00903722 neg 5.24e-13 Transcription factor that may be involved in hematopoiesis, oncogenesis, and immune responses. Plays a role in postnatal myogenesis.

Figure1 (original version):

Details are in the caption following the image
FIGURE 1
Open in figure viewerPowerPoint
Cross-validation study of multi-tissue epigenetic clock for masked shrews. Leave-one-sample-out (LOO) estimate (y-axis, in units of years) versus chronological age (x-axis, in unit of years). The linear regression of epigenetic age is indicated by a solid line while the diagonal line (y = x) is depicted by a dashed line. “MAE” represents the mean absolute error and “cor” represents the correlation coefficient.

Figure1 (corrected version):

Details are in the caption following the image
FIGURE 1
Open in figure viewerPowerPoint
Cross-validation study of multi-tissue epigenetic clock for masked shrews. Leave-one-sample-out (LOO) estimate (y-axis, in units of years) versus chronological age (x-axis, in unit of years). The linear regression of epigenetic age is indicated by a solid line while the diagonal line (y = x) is depicted by a dashed line. “MAE” represents the mean absolute error and “cor” represents the correlation coefficient. *S. fumeus samples removed.

Figure2 (original version):

Details are in the caption following the image
FIGURE 2
Open in figure viewerPowerPoint
Boxplot of A weight (g), B body length (mm) and C skull length (mm) between mainland and island masked shrew populations.

Figure2 (corrected version):

Details are in the caption following the image
FIGURE 2
Open in figure viewerPowerPoint
Boxplot of A weight (g), B body length (mm) and C skull length (mm) between mainland and island masked shrew populations. *S. fumeus samples removed.

Figure3 (original version):

Details are in the caption following the image
FIGURE 3
Open in figure viewerPowerPoint
Epigenome-wide association (EWAS) of the multivariate regression model between island and mainland masked shrew populations (location, age, sex and tissue). A Manhattan plots of the EWAS for location (island versus mainland). The coordinates are estimated based on the alignment of 29,609 Mammalian array probes to our masked shrew genome assembly. The direction of associations with < 2e-6 (red dotted line) is highlighted by red (hypermethylated) and blue (hypomethylated) colors relative to mainland methylation levels. Top 15 CpGs are indicated by their neighboring genes. B DNAm levels of island samples versus mainland for the top 10 significant CpGs (p value) associated to location. C Enrichment analysis of the top CpGs with positive (hypermethylated) and negative (hypomethylated) correlations to island populations. The gene-level enrichment analysis was carried out using the GREAT software. Background probes were limited to 14,290 probes that had shrew gene annotations. The top ontologies with most significance pathways for island samples were selected based on Bonferroni corrected p values.

Figure3 (corrected version):

Details are in the caption following the image
FIGURE 3
Open in figure viewerPowerPoint
Epigenome-wide association (EWAS) of the multivariate regression model between island and mainland masked shrew populations (location, age, sex and tissue). A Manhattan plots of the EWAS for location (island versus mainland). The coordinates are estimated based on the alignment of 29,609 Mammalian array probes to our masked shrew genome assembly. The direction of associations with < 2e-6 (red dotted line) is highlighted by red (hypermethylated) and blue (hypomethylated) colors relative to mainland methylation levels. Top 15 CpGs are indicated by their neighboring genes. B DNAm levels of island samples versus mainland for the top 10 significant CpGs (p value) associated to location. C Enrichment analysis of the top CpGs with positive (hypermethylated) and negative (hypomethylated) correlations to island populations. The gene-level enrichment analysis was carried out using the GREAT software. Background probes were limited to 14,290 probes that had shrew gene annotations. The top ontologies with most significance pathways for island samples were selected based on Bonferroni corrected p values. *S. fumeus samples removed.

Figure4 (original version):

Details are in the caption following the image
FIGURE 4
Open in figure viewerPowerPoint
Epigenome-wide association (EWAS) of the multivariate regression model between Bon Portage Island and other masked shrew populations (location, age, sex and tissue). A Manhattan plots of the EWAS for location (Bon Portage Island (BPI) versus others). The coordinates are estimated based on the alignment of 29,609 Mammalian array probes to our masked shrew genome assembly. The direction of associations with < 2e-6 (red dotted line) is highlighted by red (hypermethylated) and blue (hypomethylated) colors relative to non-BPI methylation levels. Top 15 CpGs are indicated by their neighboring genes. B DNAm levels of BPI samples versus others for the top 10 significant CpGs (p value) associated to location. C Enrichment analysis of the top CpGs with positive (hypermethylated) and negative (hypomethylated) correlations to BPI. The gene-level enrichment analysis was carried out using the GREAT software. Background probes were limited to 14,290 probes that had shrew gene annotations. The top 4 ontologies with most significance pathways for BPI were selected based on Bonferroni corrected p values.

Figure4 (corrected version):

Details are in the caption following the image
FIGURE 4
Open in figure viewerPowerPoint
Epigenome-wide association (EWAS) of the multivariate regression model between Bon Portage Island and other masked shrew populations (location, age, sex and tissue). A Manhattan plots of the EWAS for location (Bon Portage Island (BPI) versus others). The coordinates are estimated based on the alignment of 29,609 Mammalian array probes to our masked shrew genome assembly. The direction of associations with < 2e-6 (red dotted line) is highlighted by red (hypermethylated) and blue (hypomethylated) colors relative to non-BPI methylation levels. Top 15 CpGs are indicated by their neighboring genes. B DNAm levels of BPI samples versus others for the top 10 significant CpGs (p value) associated to location. C Enrichment analysis of the top CpGs with positive (hypermethylated) and negative (hypomethylated) correlations to BPI. The gene-level enrichment analysis was carried out using the GREAT software. Background probes were limited to 14,290 probes that had shrew gene annotations. The top 3 ontologies with most significance pathways for BPI were selected based on Bonferroni corrected p values. *S. fumeus samples removed.

We apologize for any inconvenience this error may have caused readers.



中文翻译:

勘误表

作者希望指出发表在《分子生态学》 (2023), 32(1), 152–166, https://doi.org/10.1111上的题为“食虫小型哺乳动物的表观遗传学和岛屿-大陆分歧”的文章中的一个错误/mec.16735。

通过评估线粒体细胞色素 B 序列数据和表型测量,我们发现数据集中的 6 个个体是Sorex fumeus,而不是S. cinereus。这包括用于参考组件和大陆比较的样本,将大陆个体的数量减少到n  = 6。这导致用于表观遗传时钟的组织样本总数从 47 个变为 39 个,用于表观遗传时钟的组织样本总数从 44 个变为 36 个用于 EWAS 分析的组织样本。参考组件仅用于识别哺乳动物阵列中的 Sorex对齐探针,并且是常见做法(例如 Larison 等人, 2021;Mayne 等人,  2022)—这不会影响分析。错误识别的样本已从数据集中删除,并重新运行所有分析。原始发表论文中的所有模式均保持不变。显示并排比较的更新表格和图如下所示。补充数据和表格也已更新。我们已更正 NCBI 上的样本记录 (https://www.ncbi.nlm.nih.gov/nuccore/JAOANZ000000000.1/)。更新的手稿作为支持信息包含在内,以便于参考。

桌子1(原始版本):

表 1.样本的捕获位置,N  = 样本总数、雌性数量、最小和最大年龄。年龄表示为估计年龄(以月为单位)除以 12。注意,年龄 0.00 代表胎儿。
地点 女性人数 年龄(最小/最大)
新斯科舍省邦波蒂奇岛 是的 16 6 0.167/1.33
新斯科舍省长岛 是的 9 4 0.00/1.25
新斯科舍省桑迪湾 4 3 0.250/0.375
新斯科舍省北山 2 1 0.417/0.417
安大略省彼得伯勒县 6 1 0.167/1.08

桌子1(修正版本):

表 1.样本的捕获位置,N  = 样本总数、雌性数量、最小和最大年龄。年龄表示为估计年龄(以月为单位)除以 12。注意,年龄 0.00 代表胎儿。 *去除了烟链霉菌样本。
地点 女性人数 年龄(最小/最大)
新斯科舍省邦波蒂奇岛 是的 16 6 0.167/1.33
新斯科舍省长岛 是的 9 4 0.00/1.25
新斯科舍省桑迪湾 4 3 0.250/0.375
新斯科舍省北山 2 1 0.417/0.417

桌子2(原始版本):

表 2.大陆蒙面鼩鼱与岛屿蒙面鼩鼱的体重、体长和头骨长度的线性回归模型的汇总统计数据。性别值与女性相关,位置与大陆人口相关。提供了β 系数(估计值)、95% 置信区间 (CI)、p值和调整后的R 2 。
估计 95%置信区间 P 调整。R 2
模型1:体重~年龄+性别+地点
年龄 1.15 0.21至2.08 0.018 0.53
性别 0.01 –0.61 至 0.64 0.962
地点 –2.09 –2.79 至 –1.39 <0.001
模型2:体长~年龄+性别+地点
年龄 8.43 3.36 至 13.50 0.002 0.49
性别 –0.20 –3.61 至 3.20 0.903
地点 –10.35 –14.15 至 –6.54 <0.001
模型3:头骨长度~年龄+性别+地点
年龄 –0.31 –0.93 至 0.32 0.321 0.79
性别 –0.21 –0.63 至 0.21 0.319
地点 –1.92 –2.39 至 –1.45 <0.001

桌子2(修正版):

表 2.大陆蒙面鼩鼱与岛屿蒙面鼩鼱的体重、体长和头骨长度的线性回归模型的汇总统计数据。性别值与女性相关,位置与大陆人口相关。提供了β 系数(估计值)、95% 置信区间 (CI)、p 值和调整后的R 2 。 *去除了烟链霉菌样本。
估计 95%置信区间 P 调整。R 2
模型1:体重~年龄+性别+地点
年龄 0.96 –0.03 至 1.95 0.056 0.27
性别 –0.21 –0.89 至 0.47 0.535
地点 –1.42 –2.26 至 –0.57 0.002
模型2:体长~年龄+性别+地点
年龄 6.95 1.27 至 12.63 0.019 0.33
性别 –0.30 –4.22 至 3.63 0.877
地点 –8.80 –13.64 至 –3.96 0.001
模型3:头骨长度~年龄+性别+地点
年龄 –0.38 –1.11 至 0.36 0.298 0.70
性别 –0.35 –0.86 至 0.16 0.170
地点 –1.64 –2.27 至 –1.01 <0.001

桌子3(原始版本):

表 3.岛屿与大陆 EWAS 和 BPI 与所有其他种群相比,靠近离群 CpG 的前 5 个基因。每个基因的功能均缩写自Uniprot。
基因名称 CPG 方向 地点(页) 假定函数
岛-大陆
EWS外显子 CG11703808 位置 3.35e-21 转录抑制因子可能在致瘤过程中发挥作用。
PO4F3内含子 CG00152260 位置 1.23e-19 转录激活剂。参与听觉系统的开发。
UBP54内含子 CG22063749 否定 3.27e-19 没有肽酶活性。
TRI47外显子 CG25115601 否定 3.12e-16 介导 CYLD 的泛素化和蛋白酶体降解。
ATPA外显子 CG24102071 否定 6.37e-16 从 ADP 产生 ATP。
BPI
SMO外显子 CG20625795 否定 1.03e-30 与补丁蛋白 (PTCH) 结合以转导刺猬蛋白信号。
ZO1外显子 CG03673751 否定 2.00e-21 与物质通过细胞旁空间的运动有关。在细胞迁移的调节中发挥作用。
PRP8启动子 CG25476805 否定 7.60e-19 在前 mRNA 剪接中发挥作用。
CBX5基因间 CG23036219 否定 1.12e-18 与表观遗传抑制有关。参与功能着丝粒的形成。
ZBT20基因间 CG00903722 否定 3.32e-17 可能参与造血、肿瘤发生和免疫反应的转录因子。在产后肌生成中发挥作用。

桌子3(修正版):

表 3.岛屿与大陆 EWAS 和 BPI 与所有其他种群相比,靠近离群 CpG 的前 5 个基因。每个基因的功能均缩写自Uniprot。 *去除了烟链霉菌样本。
基因名称 CPG 方向 地点(页) 假定函数
岛-大陆
EWS外显子 CG11703808 位置 5.76e-18 转录抑制因子可能在致瘤过程中发挥作用。
PO4F3内含子 CG00152260 位置 7.32e-14 转录激活剂。参与听觉系统的开发。
UBP54内含子 CG22063749 否定 1.71e-13 没有肽酶活性。
MYH6外显子 CG11736686 位置 5.48e-12 具有 ATP 酶活性的基于肌动蛋白的运动分子。
TRI47外显子 CG25115601 否定 2.07e-11 介导 CYLD 的泛素化和蛋白酶体降解。
BPI
SMO外显子 CG20625795 否定 1.06e-24 与补丁蛋白 (PTCH) 结合以转导刺猬蛋白信号。
ZO1外显子 CG03673751 否定 6.04e-16 与物质通过细胞旁空间的运动有关。在细胞迁移的调节中发挥作用。
PRP8启动子 CG25476805 否定 2.57e-14 在前 mRNA 剪接中发挥作用。
CBX5基因间 CG23036219 否定 1.10e-13 与表观遗传抑制有关。参与功能着丝粒的形成。
ZBT20基因间 CG00903722 否定 5.24e-13 可能参与造血、肿瘤发生和免疫反应的转录因子。在产后肌生成中发挥作用。

数字1(原始版本):

详细信息位于图片后面的标题中
图1
在图查看器中打开微软幻灯片软件
蒙面鼩鼱多组织表观遗传时钟的交叉验证研究。留一样本 (LOO) 估计值(y 轴,以年为单位)与实际年龄(x 轴,以年为单位)。表观遗传年龄的线性回归由实线表示,而对角线(y = x)由虚线表示。 “MAE”代表平均绝对误差,“cor”代表相关系数。

数字1(修正版本):

详细信息位于图片后面的标题中
图1
在图查看器中打开微软幻灯片软件
蒙面鼩鼱多组织表观遗传时钟的交叉验证研究。留一样本 (LOO) 估计值(y 轴,以年为单位)与实际年龄(x 轴,以年为单位)。表观遗传年龄的线性回归由实线表示,而对角线(y = x)由虚线表示。 “MAE”代表平均绝对误差,“cor”代表相关系数。 *S。去除烟气样品。

数字2(原始版本):

详细信息位于图片后面的标题中
图2
在图查看器中打开微软幻灯片软件
大陆和岛屿蒙面鼩鼱种群之间的 A 体重 (g)、B 体长 (mm) 和 C 头骨长度 (mm) 的箱线图。

数字2(修正版):

详细信息位于图片后面的标题中
图2
在图查看器中打开微软幻灯片软件
大陆和岛屿蒙面鼩鼱种群之间的 A 体重 (g)、B 体长 (mm) 和 C 头骨长度 (mm) 的箱线图。 *S。去除烟气样品。

数字3(原始版本):

详细信息位于图片后面的标题中
图3
在图查看器中打开微软幻灯片软件
岛屿和大陆蒙面鼩鼱种群之间的多元回归模型的全表观基因组关联(EWAS)(位置、年龄、性别和组织)。曼哈顿 EWAS 位置图(岛屿与大陆)。坐标是根据 29,609 个哺乳动物阵列探针与我们的蒙面鼩鼱基因组组装的比对来估计的。与大陆甲基化水平相关的< 2e-6(红色虚线)的关联方向通过红色(高甲基化)和蓝色(低甲基化)颜色突出显示。前 15 个 CpG 由其邻近基因表示。 B 与位置相关的前 10 个重要 CpG(p值)的岛屿样本与大陆样本的 DNAm 水平。 C 对与岛屿种群具有正相关(高甲基化)和负相关(低甲基化)的顶级 CpG 进行富集分析。使用GREAT软件进行基因水平富集分析。背景探针仅限于 14,290 个具有鼩鼱基因注释的探针。根据 Bonferroni 校正p值选择对岛屿样本具有最重要路径的顶级本体。

数字3(修正版):

详细信息位于图片后面的标题中
图3
在图查看器中打开微软幻灯片软件
岛屿和大陆蒙面鼩鼱种群之间的多元回归模型的全表观基因组关联(EWAS)(位置、年龄、性别和组织)。曼哈顿 EWAS 位置图(岛屿与大陆)。坐标是根据 29,609 个哺乳动物阵列探针与我们的蒙面鼩鼱基因组组装的比对来估计的。与大陆甲基化水平相关的< 2e-6(红色虚线)的关联方向通过红色(高甲基化)和蓝色(低甲基化)颜色突出显示。前 15 个 CpG 由其邻近基因表示。 B 与位置相关的前 10 个重要 CpG(p值)的岛屿样本与大陆样本的 DNAm 水平。 C 对与岛屿种群具有正相关(高甲基化)和负相关(低甲基化)的顶级 CpG 进行富集分析。使用GREAT软件进行基因水平富集分析。背景探针仅限于 14,290 个具有鼩鼱基因注释的探针。根据 Bonferroni 校正的p值选择对岛屿样本具有最重要路径的顶级本体。 *S。去除烟气样品。

数字4(原始版本):

详细信息位于图片后面的标题中
图4
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Bon Portage 岛和其他蒙面鼩鼱种群之间的多元回归模型的表观基因组范围关联 (EWAS)(位置、年龄、性别和组织)。曼哈顿 EWAS 位置图(邦波蒂奇岛 (BPI) 与其他岛)。坐标是根据 29,609 个哺乳动物阵列探针与我们的蒙面鼩鼱基因组组装的比对来估计的。相对于非 BPI 甲基化水平,与 < 2e-6(红色虚线)的关联方向通过红色(高甲基化)和蓝色(低甲基化)颜色突出显示。前 15 个 CpG 由其邻近基因表示。 B BPI 样本与其他样本的 DNAm 水平,与位置相关的前 10 个重要 CpG(p值)。 C 对与 BPI 呈正相关(高甲基化)和负相关(低甲基化)的顶级 CpG 进行富集分析。使用GREAT软件进行基因水平富集分析。背景探针仅限于 14,290 个具有鼩鼱基因注释的探针。根据 Bonferroni 校正的p值选择对 BPI 最重要的路径的前 4 个本体。

数字4(修正版):

详细信息位于图片后面的标题中
图4
在图查看器中打开微软幻灯片软件
Bon Portage 岛和其他蒙面鼩鼱种群之间的多元回归模型的全表观基因组关联 (EWAS)(位置、年龄、性别和组织)。曼哈顿 EWAS 位置图(邦波蒂奇岛 (BPI) 与其他岛)。坐标是根据 29,609 个哺乳动物阵列探针与我们的蒙面鼩鼱基因组组装的比对来估计的。相对于非 BPI 甲基化水平,与 < 2e-6(红色虚线)的关联方向通过红色(高甲基化)和蓝色(低甲基化)颜色突出显示。前 15 个 CpG 由其邻近基因表示。 B BPI 样本与其他样本的 DNAm 水平,与位置相关的前 10 个重要 CpG(p值)。 C 对与 BPI 呈正相关(高甲基化)和负相关(低甲基化)的顶级 CpG 进行富集分析。使用GREAT软件进行基因水平富集分析。背景探针仅限于 14,290 个具有鼩鼱基因注释的探针。根据 Bonferroni 校正的p值选择对 BPI 最重要的路径的前 3 个本体。 *S。去除烟气样品。

对于此错误可能给读者带来的任何不便,我们深表歉意。

更新日期:2024-04-22
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