Jasmonate (JA) is the important phytohormone to regulate plant growth and adaption to stress signals. MYC2, an bHLH transcription factor, is the master regulator of JA signaling. Although MYC2 in maize has been identified, its function remains to be clarified. To understand the function and regulatory mechanism of MYC2 in maize, the joint analysis of DAP-seq and RNA-seq is conducted to identify the binding sites and target genes of ZmMYC2. A total of 3183 genes are detected both in DAP-seq and RNA-seq data, potentially as the directly regulating genes of ZmMYC2. These genes are involved in various biological processes including plant growth and stress response. Besides the classic cis-elements like the G-box and E-box that are bound by MYC2, some new motifs are also revealed to be recognized by ZmMYC2, such as nGCATGCAnn, AAAAAAAA, CACGTGCGTGCG. The binding sites of many ZmMYC2 regulating genes are identified by IGV-sRNA. All together, abundant target genes of ZmMYC2 are characterized with their binding sites, providing the basis to construct the regulatory network of ZmMYC2 and better understanding for JA signaling in maize.

中文翻译：

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玉米 ZmMYC2 结合位点和靶基因的全基因组鉴定

茉莉酸（JA）是调节植物生长和适应胁迫信号的重要植物激素。 MYC2 是一种 bHLH 转录因子，是 JA 信号传导的主要调节因子。尽管玉米中的MYC2已被鉴定，但其功能仍有待阐明。为了了解玉米中MYC2的功能和调控机制，通过DAP-seq和RNA-seq联合分析来鉴定ZmMYC2的结合位点和靶基因。 DAP-seq 和 RNA-seq 数据中总共检测到 3183 个基因，可能是 ZmMYC2 的直接调控基因。这些基因参与各种生物过程，包括植物生长和应激反应。除了MYC2结合的G-box和E-box等经典顺式元件外，ZmMYC2还识别了一些新的基序，例如nGCATGCAnn、AAAAAAAA、CACGTGCGTGCG。许多 ZmMYC2 调节基因的结合位点由 IGV-sRNA 识别。总之，ZmMYC2 丰富的靶基因及其结合位点的特征，为构建 ZmMYC2 调控网络和更好地理解玉米中的 JA 信号传导提供了基础。