The effect of chitosan conjugates with caffeic (ChCA) and ferulic (ChFA) acids in combination with Bacillus subtilis bacteria on the transcriptional activity of PR protein genes and proteome changes in potato plants during infection with Phytophthora infestans (Mont.) de Bary was studied. Plants grown from mini tubers of the Udacha variety were sprayed with solutions of ChCA and ChFA, a suspension of B. subtilis bacteria strains 26D and 11 VM, and conjugates of ChCA of ChFA together with bacteria. At 3 days after treatment, some plants were infected with P. infestans. A decrease in the degree of development of the late blight pathogen on potato leaves in all treatment options was revealed. The maximum protective effect was manifested when plants were treated with the B. subtilis strain 26D bacteria in combination with conjugates of chitosan and oxycinnamic acids. The mechanisms of increasing the resistance of potato plants to P. infestans were associated with the activation of transcriptional activity of genes encoding the main protective protein (PR-1), chitinase (PR-3), thaumatin-like protein (PR-5), protease inhibitor (PR-6), peroxidase (PR-9), and ribonuclease (PR-10). The revealed activation of the expression of marker genes of systemic acquired resistance and induced systemic resistance under the influence of joint treatment of plants with B. subtilis and chitin conjugates with hydroxycinnamic acids indicates the synergistic development of protective reactions in potato plants in this variant. Using the method of two-dimensional electrophoresis of S. tuberosum leaf proteins followed by MALDI-TOF analysis 12 proteins were identified; their presence in the leaves differed depending on the variant of the experiment. In all treatment variants, serine–threonine protein phosphatase activity was suppressed, reflecting the development of the hypersensitivity reaction. Different variants of the experiment formed weakly expressed clusters, which indicates multiple mechanisms of regulation of the synthesis of protective proteins involved in the reaction to treatment with bacteria, chitosan conjugates, and infection with P. infestans.

研究了壳聚糖与咖啡酸 (ChCA) 和阿魏酸 (ChFA) 结合物与枯草芽孢杆菌细菌结合对马铃薯植株感染致病疫霉 ( Phytophthora infestans (Mont.) de Bary ) 期间 PR 蛋白基因转录活性和蛋白质组变化的影响。用 ChCA 和 ChFA 溶液、枯草芽孢杆菌细菌菌株 26D 和 11 VM 的悬浮液以及 ChCA 和 ChFA 与细菌的缀合物喷洒从 Udacha 品种的小块茎生长的植物。处理后3天，一些植物感染了致病疫霉。结果表明，在所有处理方案中，马铃薯叶片上晚疫病病原体的发育程度均有所下降。当用枯草芽孢杆菌菌株 26D 细菌与壳聚糖和羟基肉桂酸的缀合物组合处理植物时，表现出最大的保护效果。马铃薯植株对致病疫霉抗性增强的机制与主要保护蛋白（PR-1）、几丁质酶（PR-3）、索马甜蛋白（PR-5）编码基因转录活性的激活有关。 、蛋白酶抑制剂 (PR-6)、过氧化物酶 (PR-9) 和核糖核酸酶 (PR-10)。在枯草芽孢杆菌和羟基肉桂酸甲壳素缀合物联合处理植物的影响下，系统获得性抗性和诱导系统性抗性的标记基因表达的激活表明该变体中马铃薯植物中保护反应的协同发展。采用马铃薯叶蛋白二维电泳及MALDI-TOF分析方法，鉴定出12种蛋白；它们在叶子中的存在因实验的不同而不同。在所有治疗方案中，丝氨酸-苏氨酸蛋白磷酸酶活性均受到抑制，反映了超敏反应的发展。该实验的不同变体形成了弱表达的簇，这表明保护性蛋白的合成调节涉及细菌、壳聚糖缀合物处理和致病疫霉感染的反应的多种机制。