Endophytic bacteria possess a range of unique characteristics that enable them to successfully interact with their host and survive in adverse environments. This study employed in silico analysis to identify genes, from Bacillus sp. strain MHSD_37, with potential biotechnological applications. The strain presented several endophytic lifestyle genes which encode for motility, quorum sensing, stress response, desiccation tolerance and root colonisation. The presence of plant growth promoting genes such as those involved in nitrogen fixation, nitrate assimilation, siderophores synthesis, seed germination and promotion of root nodule symbionts, was detected. Strain MHSD_37 also possessed genes involved in insect virulence and evasion of defence system. The genome analysis also identified the presence of genes involved in heavy metal tolerance, xenobiotic resistance, and the synthesis of siderophores involved in heavy metal tolerance. Furthermore, LC-MS analysis of the excretome identified secondary metabolites with biological activities such as anti-cancer, antimicrobial and applications as surfactants. Strain MHSD_37 thereby demonstrated potential biotechnological application in bioremediation, biofertilisation and biocontrol. Moreover, the strain presented genes encoding products with potential novel application in bio-nanotechnology and pharmaceuticals.

中文翻译：

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芽孢杆菌的计算机生物技术潜力。菌株 MHSD_37 细菌内生菌

内生细菌具有一系列独特的特性，使它们能够成功地与宿主相互作用并在不利的环境中生存。这项研究采用计算机分析来鉴定来自芽孢杆菌属的基因。菌株 MHSD_37，具有潜在的生物技术应用。该菌株呈现出几种内生生活方式基因，这些基因编码运动性、群体感应、应激反应、耐干燥性和根定殖。检测到植物生长促进基因的存在，例如涉及固氮、硝酸盐同化、铁载体合成、种子萌发和促进根瘤共生体的基因。 MHSD_37菌株还拥有与昆虫毒力和逃避防御系统有关的基因。基因组分析还确定了与重金属耐受性、外源性抗性以及与重金属耐受性相关的铁载体的合成有关的基因的存在。此外，排泄物的 LC-MS 分析确定了具有抗癌、抗菌和表面活性剂应用等生物活性的次级代谢物。因此，菌株 MHSD_37 证明了其在生物修复、生物施肥和生物防治方面的潜在生物技术应用。此外，该菌株呈现的基因编码产物在生物纳米技术和制药方面具有潜在的新应用。