We show that the mitochondrial fission proteins MiD49 and MiD51 are activated by fatty acyl-coenzyme A (FA-CoA). FA-CoA binds in a previously identified pocket located within MiDs, inducing their oligomerization and ability to activate the dynamin DRP1, ultimately promoting mitochondrial fission. Activated MiDs synergize with mitochondrial fission factor (MFF) in stimulating DRP1 activity, leading us to hypothesize that MiDs act upstream of MFF during mitochondrial fission.

中文翻译：

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脂酰辅酶 A 对线粒体裂变的调节

我们发现线粒体裂变蛋白 MiD49 和 MiD51 被脂肪酰辅酶 A (FA-CoA) 激活。 FA-CoA 结合在先前确定的 MiD 内的口袋中，诱导其寡聚化并具有激活动力 DRP1 的能力，最终促进线粒体裂变。激活的 MiD 与线粒体裂变因子 (MFF) 协同刺激 DRP1 活性，这使我们推测 MiD 在线粒体裂变过程中在 MFF 的上游发挥作用。