A novel putative L-arabinose isomerase (L-AI) called ApL-AI with the accession number WP_110486392.1 was successfully retrieved from Arthrobacter psychrolactophilus B7 genome (Accession: NZ_QJVC01000021.1) through genome mining analysis. This study aimed to obtain the L-AI gene from the Arthrobacter psychrolactophilus B7 genome and clone it into the pET28a(+) plasmid. The primers pair designed in this study successfully amplified the gene using 60 °C of PCR annealing temperature and supported the gene amplicon to insert into the pET28a(+) to form plasmid pET28a(+)-ApLAI. It was proved by the appearance of a 1557-bp amplification band on the gel electrophoresis. The sequencing analysis also revealed that the gene was inserted in the correct direction, with the gene positioned after the promoter and finished with a terminator. Therefore, the plasmid can be used to express the ApL-AI gene to produce the ApL-AI enzyme for downstream analysis and further prospecting.

中文翻译：

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冷乳节杆菌L-阿拉伯糖异构酶基因克隆入质粒pET28a(+)的引物设计

成功检索到一种新型假定的 L-阿拉伯糖异构酶（L-AI），称为 ApL-AI，登录号为 WP_110486392.1嗜冷乳节杆菌B7基因组（登录号：NZ_QJVC01000021.1）通过基因组挖掘分析。本研究旨在从植物中获得L-AI基因。嗜冷乳节杆菌B7 基因组并将其克隆到 pET28a(+) 质粒中。本研究设计的引物对在60℃的PCR退火温度下成功扩增了该基因，并支持该基因扩增子插入pET28a(+)中，形成质粒pET28a(+)-ApLAI。凝胶电泳上出现1557bp的扩增带证明了这一点。测序分析还显示该基因以正确的方向插入，该基因位于启动子之后并以终止子结束。因此，该质粒可用于表达ApL-AI基因，产生ApL-AI酶用于下游分析和进一步探索。