Single microbial cell oil (SMCO) plays a fundamental role in maintaining the structure of cellular membranes and as energy reservoirs. Oleaginous microbes are known to have a high content of lipids, which often acts as a microbial factory for the bioremediation of waste cooking oil (WCO) which is a major pollutant contaminating land and water bodies. The biomass of microbes grown in WCO can be utilized to develop sustainable value-added products such as biodiesel, organic chemicals, bio preservatives, biosurfactants biopolymers. Conventional methods excel in SMCO analysis but lack efficacy for in situ or lysis-free monitoring of nascent SMCO synthesis and its turnover dynamics. To bridge this knowledge gap, in this study we have employed Raman reverse stable isotope probing (RrSIP) to investigate the time dependent nascent SMCO synthesis and assimilation in Yarrowia lipolytica, a well-known oleaginous yeast. Our finding provides a unique perspective for utilizing optical spectroscopy methods for lysis-free SMCO analysis which holds potential for utilization as an adjunct tool in bioprocess and biofuels industries.

中文翻译：

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追踪垃圾到宝藏：利用拉曼反向同位素探测和成像对废弃食用油中单微生物细胞油的生物合成进行原位监测

单微生物细胞油 (SMCO) 在维持细胞膜结构和作为能量储存库方面发挥着重要作用。已知油质微生物具有高脂质含量，通常充当废食用油（WCO）生物修复的微生物工厂，废食用油是污染土地和水体的主要污染物。 WCO 中生长的微生物生物质可用于开发可持续的增值产品，如生物柴油、有机化学品、生物防腐剂、生物表面活性剂生物聚合物。传统方法在 SMCO 分析方面表现出色，但缺乏对新生 SMCO 合成及其周转动态的原位或免裂解监测的功效。为了弥补这一知识差距，在本研究中，我们采用拉曼反向稳定同位素探测 (RrSIP) 来研究解脂耶氏酵母（一种著名的产油酵母）中时间依赖性的新生 SMCO 合成和同化。我们的发现为利用光谱方法进行无裂解 SMCO 分析提供了独特的视角，该方法具有作为生物过程和生物燃料行业的辅助工具的潜力。