Abstract

Surface plasmon resonance (SPR)-based biosensors have emerged as a powerful platform for bioprocess monitoring due to their ability to detect biointeractions in real time, without the need for labeling. Paramount for the development of a robust detection platform is the immobilization of a ligand with high specificity and affinity for the in-solution species of interest. Following the 2009 H1N1 pandemic, much effort has been made toward the development of quality control platforms for influenza A vaccine productions, many of which have employed SPR for detection. Due to the rapid antigenic drift of influenza’s principal surface protein, hemagglutinin, antibodies used for immunoassays need to be produced seasonally. The production of these antibodies represents a 6–8-week delay in immunoassay and, thus, vaccine availability. This review focuses on SPR-based assays that do not rely on anti-HA antibodies for the detection, characterization, and quantification of influenza A in bioproductions and biological samples.

Key points

• The single radial immunodiffusion assay (SRID) has been the gold standard for the quantification of influenza vaccines since 1979. Due to antigenic drift of influenza’s hemagglutinin protein, new antibody reagents for the SRID assay must be produced each year, requiring 6–8 weeks. The resulting delay in immunoassay availability is a major bottleneck in the influenza vaccine pipeline. This review highlights ligand options for the detection and quantification of influenza viruses using surface plasmon resonance biosensors.

中文翻译：

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用于流感疫苗质量控制的不依赖于抗体的表面等离子共振测定

摘要

基于表面等离子共振 (SPR) 的生物传感器已成为生物过程监测的强大平台，因为它们能够实时检测生物相互作用，而无需标记。开发强大的检测平台最重要的是固定对感兴趣的溶液内物种具有高特异性和亲和力的配体。 2009年H1N1流感大流行后，甲型流感疫苗生产质量控制平台的开发投入了大量精力，其中许多都采用了SPR进行检测。由于流感主要表面蛋白血凝素的快速抗原漂移，用于免疫测定的抗体需要季节性生产。这些抗体的产生意味着免疫测定延迟了 6-8 周，因此疫苗的可用性也延迟了 6-8 周。本综述重点关注基于 SPR 的检测，这些检测不依赖抗 HA 抗体来检测、表征和定量生物生产和生物样品中的甲型流感病毒。

关键点

•自1979年以来，单径向免疫扩散测定（SRID）一直是流感疫苗定量的金标准。由于流感血凝素蛋白的抗原漂移，每年必须生产用于SRID测定的新抗体试剂，需要6-8周的时间。由此导致的免疫测定可用性的延迟是流感疫苗研发过程中的一个主要瓶颈。本综述重点介绍了使用表面等离子共振生物传感器检测和定量流感病毒的配体选项。