At present, understanding of neonicotinoid toxicity in arthropods remains limited. We here evaluated the lethal and sublethal effects of acetamiprid in F₀ and F₁ generations of Binodoxys communis using a range of sublethal concentrations. The 10% lethal concentration (LC₁₀) and half lethal concentration (LC₂₅) of ACE had negative effects on the B. communis survival rate, adult longevity, parasitism rate, and emergence rate, and significantly prolonged the duration of the developmental cycle. ACE also had intergenerational effects, with some biological indices affected in the F₁ generation after pesticide exposure. Transcriptomic analysis demonstrated that differentially expressed genes were enriched in specific pathways including the amino acid metabolism, carbohydrate metabolism, energy metabolism, exogenous metabolism, signal transduction, and glutathione metabolism pathways. These results indicated strong contact toxicity of ACE to B. communis, which may inhibit their biological control capacity. These results improve our understanding of the toxicological mechanisms of parasitic natural enemies in response to insecticide exposure.

目前，对新烟碱类杀虫剂对节肢动物的毒性的了解仍然有限。我们在此使用一系列亚致死浓度评估了啶虫脒对 F ₀和 F ₁代Binodoxys commis的致死和亚致死作用。 ACE的10%致死浓度(LC ₁₀ )和半致死浓度(LC _{25 )对}双歧杆菌的存活率、成虫寿命、寄生率和羽化率产生负面影响，并显着延长发育周期的持续时间。 ACE还具有代际效应，F ₁代的一些生物学指标在接触农药后受到影响。转录组分析表明，差异表达基因富集于特定途径，包括氨基酸代谢、碳水化合物代谢、能量代谢、外源代谢、信号转导和谷胱甘肽代谢途径。这些结果表明ACE对双歧杆菌有很强的接触毒性，这可能会抑制其生物防治能力。这些结果提高了我们对寄生天敌响应杀虫剂暴露的毒理学机制的理解。