The remarkable antibacterial activity and potential biomedical applications of silver nanoparticles (AgNPs) synthesized through eco-friendly approaches have garnered significant attention in recent years This research aimed to introduce a green synthesis method for AgNPs using Bunge extract and to investigate their properties, antibacterial activities, and cytotoxicity effects. The fabricated AgNPs were analyzed with various analyses, including UV–Vis, DLS, XRD, FT-IR, and TEM analysis. The broth microdilution assay was applied to measure the Minimum Inhibitory Concentrations (MIC) of chemical AgNPs, biosynthesized AgNPs, and Bunge leaves extract alone against selected standard bacteria strains. The biosynthesized AgNPs displayed a surface plasmon resonance peak at approximately 415 nm, and the AgNPs synthesized using Bunge extract had a spherical configuration with an average size in the range of 10–15 nm. Biosynthesized nanoparticles showed a noteworthy antibacterial activity compared to chemical nanoparticles, particularly against , with the highest antibacterial activity reported at a MIC value of 39.06 μg/mL. AgNPs synthesized using extract demonstrated a significant decrease in fibroblast cell viability, but only when the concentration reached 2 mg/mL. The findings demonstrate that Bunge leaves extract enhances the antibacterial properties of AgNPs, and also represents an appropriate and biocompatible option for the synthesis of these nanoparticles. Our research highlights the potential of employing bio-safe and eco-friendly AgNPs synthesized in the presence of Bunge extract, which possess remarkable antibacterial properties, for various biomedical applications.

中文翻译：

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使用丹参提取物绿色合成无毒银纳米颗粒：优化、细胞毒性和抗菌活性

近年来，通过环保方法合成的纳米银（AgNP）卓越的抗菌活性和潜在的生物医学应用引起了人们的广泛关注。本研究旨在介绍一种利用邦吉提取物绿色合成纳米银的方法，并研究其特性、抗菌活性、和细胞毒性作用。通过多种分析方法对制备的 AgNP 进行了分析，包括 UV-Vis、DLS、XRD、FT-IR 和 TEM 分析。应用肉汤微量稀释测定来测量化学纳米银颗粒、生物合成纳米银颗粒和单独的邦吉叶提取物对选定标准细菌菌株的最低抑制浓度 (MIC)。生物合成的 AgNP 在约 415 nm 处显示出表面等离子共振峰，使用 Bunge 提取物合成的 AgNP 具有球形结构，平均尺寸在 10-15 nm 范围内。与化学纳米颗粒相比，生物合成纳米颗粒显示出显着的抗菌活性，特别是针对 ，据报道抗菌活性最高，MIC 值为 39.06 μg/mL。使用提取物合成的 AgNP 表明成纤维细胞活力显着降低，但仅当浓度达到 2 mg/mL 时才发生。研究结果表明，白松叶提取物增强了 AgNP 的抗菌特性，并且也代表了合成这些纳米颗粒的适当且生物相容的选择。我们的研究强调了在 Bunge 提取物存在下合成的生物安全且环保的 AgNP 的潜力，该纳米颗粒具有显着的抗菌特性，可用于各种生物医学应用。