The potential of microbial fuel cells (MFCs) based biosensors for water monitoring and early warning is widely recognized. However, the electrochemically active biofilms (EABs) are typically obtained under anaerobic conditions and the formation requires a lengthy time span, which critically limits the application and advancement of the MFCs based monitoring technology. To accelerate the formation rate of EABs and thus shorten the establishment time of MFCs to better meet the needs for early warning and detection of pollutants in the actual aerobic water environment, the aerobic sludge was employed as an inoculation source to construct the MFCs sensing system in this study. It was revealed that biofilms with stable electrochemical properties could be promptly formed within 35 h, reaching a level far ahead of previous studies. Moreover, toxicity tests for copper (Cu) ions at concentrations of 1 mg/L, 10 mg/L, and 50 mg/L demonstrated outstanding performance with the maximum inhibition rates of 19.99%, 46.65%, and 53.02% respectively. Remarkably, the response time was found to decrease significantly with the increase of copper ion concentrations. Our results thus open up a new avenue for achieving rapid start-up of MFCs in aerobic conditions, which facilitates the applicability of MFCs based biosensing technology.

中文翻译：

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用于铜离子检测的创新快速启动好氧阳极微生物燃料电池生物传感器

基于微生物燃料电池（MFC）的生物传感器在水监测和预警方面的潜力已得到广泛认可。然而，电化学活性生物膜（EAB）通常是在厌氧条件下获得的，并且形成需要较长的时间跨度，这严重限制了基于MFC的监测技术的应用和进步。为了加快EABs的形成速度，从而缩短MFCs的建立时间，更好地满足实际好氧水环境中污染物预警和检测的需要，以好氧污泥为接种源，构建MFCs传感系统。这项研究。结果表明，具有稳定电化学性能的生物膜可以在35小时内迅速形成，达到了远远领先于之前研究的水平。此外，对1 mg/L、10 mg/L和50 mg/L浓度的铜（Cu）离子的毒性测试显示出优异的性能，最大抑制率分别为19.99%、46.65%和53.02%。值得注意的是，随着铜离子浓度的增加，响应时间显着缩短。因此，我们的结果为在有氧条件下实现 MFC 的快速启动开辟了一条新途径，这促进了基于 MFC 的生物传感技术的适用性。