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Phosphoproteomic profiling identifies DNMT1 as a key substrate of beta IV spectrin-dependent ERK/MAPK signaling in suppressing angiogenesis Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-10 Paola Cruz Flores, Tasmia Ahmed, Julia Podgorski, Hannah R. Ortiz, Paul R. Langlais, Karthikeyan Mythreye, Nam Y. Lee
βIV-spectrin is a membrane-associated cytoskeletal protein that maintains the structural stability of cell membranes and integral proteins such as ion channels and transporters. Its biological functions are best characterized in the brain and heart, although recently we discovered a fundamental new role in the vascular system. Using cellular and genetic mouse models, we reported that βIV-spectrin acts
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Acesulfame potassium upregulates PD-L1 in HCC cells by attenuating autophagic degradation Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-09 Dong-Ho Kim, Eun-Jun Kwon, Keun-Gyu Park, Jonghwa Jin, Jun-Kyu Byun
Artificial sweeteners, which contain no or few calories, have been widely used in various foods and beverages, and are regarded as safe alternatives to sugar by the Food and Drug Administration. While several studies suggest that artificial sweeteners are not related to cancer development, some research has reported their potential association with the risk of cancers, including hepatocellular carcinoma
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Roles of HIF-1α signaling in Mycobacterium tuberculosis infection: New targets for anti-TB therapeutics? Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-09 Chaowei Li, Jiajun Wang, Jun-Fa Xu, Jiang Pi, Biying Zheng
Tuberculosis (TB), a deadly infectious disease induced by (Mtb), continues to be a global public health issue that kill millions of patents every year. Despite significant efforts have been paid to identify effective TB treatments, the emergence of drug-resistant strains of the disease and the presence of comorbidities in TB patients urges us to explore the detailed mechanisms involved in TB immunity
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Linagliptin decreased the tumor progression on glioblastoma model Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-09 Shohei Tsuji, Urara Kudo, Ryo Hatakeyama, Kenji Shoda, Shinsuke Nakamura, Masamitsu Shimazawa
Dipeptidyl peptidase-4 (DPP-4) inhibitors are oral hypoglycemic drugs and are used for type II diabetes. Previous studies showed that DPP-4 expression is observed in several tumor types and DPP-4 inhibitors suppress the tumor progression on murine tumor models. In this study, we evaluated the role of DPP-4 and the antitumor effect of a DPP-4 inhibitor, linagliptin, on glioblastoma (GBM). We analyzed
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Adjuvant-free parenterally injectable vaccine platform that harnesses previously induced IgG as an antigen delivery carrier Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-07 Atsushi Kawai, Haruki Hirata, Nagisa Tokunoh, Chikako Ono, Yoshiharu Matsuura, Toshiro Hirai, Yasuo Yoshioka
Subunit vaccines are among the most useful vaccine modalities; however, their low immunogenicity necessitates the addition of adjuvants. Although adjuvants improve immune responses induced by vaccines, they often cause adverse reactions. To address this, we developed an adjuvant-free subunit vaccine platform that uses pre-existing antibodies generated from past infections or vaccinations as carriers
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Supreme glutathione-dependent ketosteroid isomerase in the yellow-fever transmitting mosquito Aedes aegypti Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-07 Katarína Šťastná, Yaman Musdal, Aram Ismail, Kana Ebihara, Ryusuke Niwa, Bengt Mannervik
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Eliminating extracellular autoinducing peptide signals inhibits the Staphylococcus aureus quorum sensing agr system Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-07 Ruki Inagaki, Ayaka Koshiba, Eri Nasuno, Norihiro Kato
An accessory gene regulator () in the quorum sensing (QS) system in contributes to host infection, virulence factor production, and resistance to oxidative damage. Artificially maintaining the inactive state of QS impedes the host infection strategy of and inhibits toxin production. The QS system performs intercellular signal transduction, which is activated by the mature autoinducer peptide (AIP)
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Synergistic effects of graphene microgrooves and electrical stimulation on M2 macrophage polarization Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-07 Yan Yan, Yilin Zhang, Kexin Li, Yudong Li, Wei Qian, Wenbo Zhang, Yue Wang, Wensheng Ma, Linhao Li
Macrophages play a crucial role in host response and wound healing, with M2 polarization contributing to the reduction of foreign-body reactions induced by the implantation of biomaterials and promoting tissue regeneration. Electrical stimulation (ES) and micropatterned substrates have a significant impact on the macrophage polarization. However, there is currently a lack of well-established cell culture
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Biophysical characterization and design of a minimal version of the Hoechst RNA aptamer Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-07 Natasha M. Evans, Lindsey R. Shivers, Avery J. To, Graham K. Murphy, Thorsten Dieckmann
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A novel MICA/B-targeted chimeric antigen receptor augments the cytotoxicity of NK cells against tumor cells Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-07 Changjiang Guo, Meng Dong, Xiang Wang, Jie Yu, Xinru Jin, Shizhuang Cheng, Feiyan Cui, Yifan Qian, Qianqian Bao, Lingtong Zhi, Zhiyuan Niu, Mingfeng Li, Wuling Zhu
Chimeric antigen receptor (CAR)-modified immune cells have emerged as a promising approach for cancer treatment, but single-target CAR therapy in solid tumors is limited by immune escape caused by tumor antigen heterogeneity and shedding. Natural killer group 2D (NKG2D) is an activating receptor expressed in human NK cells, and its ligands, such as MICA and MICB (MICA/B), are widely expressed in malignant
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Melanin-concentrating hormone receptor 1 is discarded by exosomes after internalization Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-07 Ryohei Yamada, Momoka Michimae, Akie Hamamoto, Hiroshi Takemori
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Ginsenoside Rb1 attenuates doxorubicin induced cardiotoxicity by suppressing autophagy and ferroptosis Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-07 Yafei Zhai, Jinmeng Bai, Ying Peng, Jinhua Cao, Guangming Fang, Yiming Dong, Ze Wang, Yanyu Lu, Mengyu Wang, Mengduan Liu, Yangyang Liu, Xiaowei Li, Jianzeng Dong, Xiaoyan Zhao
Ginsenoside Rb1 (Rb1), an active component isolated from traditional Chinese medicine Ginseng, is beneficial to many cardiovascular diseases. However, whether it can protect against doxorubicin induced cardiotoxicity (DIC) is not clear yet. In this study, we aimed to investigate the role of Rb1 in DIC. Mice were injected with a single dose of doxorubicin (20 mg/kg) to induce acute cardiotoxicity. Rb1
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A dynamic regulatory switch for phase separation of FUS protein: Zinc ions and zinc finger domain Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-07 Yatao Chen, Xiaoying Pei, Long Chen, Liming Chen
Zinc is an important trace element in the human body, and its homeostasis is closely related to amyotrophic lateral sclerosis (ALS). Cytoplasmic FUS proteins from patients with ALS aggregate their important pathologic markers. Liquid-liquid phase separation (LLPS) of FUS can lead to its aggregation. However, whether and how zinc homeostasis affects the aggregation of disease-associated FUS proteins
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Evaluation of reverse transcription yield of RNA standards and forensic samples based on droplet digital PCR Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-06 Niu Gao, Yumei Huo, Daijing Yu, Feng Cheng, Tian Wang, Xiaomeng Zhang, Liwei Zhang, Wenjing Hu, Junli Li, Piao Yuan, Jinding Liu, Yan Wang, Jiangwei Yan
RNA analysis has shown great value in forensic science, such as body fluids and tissue identification, postmortem interval estimation, biological age prediction, etc. Currently, most RNA follow-up experiments involve reverse transcription (RT) procedures. It has been shown that the RT step is variable and has a greater impact on subsequent data analysis, especially for forensic trace samples. However
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Chronic restraint stress-induced hyperalgesia is modulated by the periaqueductal gray neurons projecting to the rostral ventromedial medulla in mice Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-06 Qian Chen, Mingwei Zhao, Jiaxue Dong, Kun Yang
Stress-induced hyperalgesia (SIH) is induced by repeated or chronic exposure to stressful or uncomfortable environments. However, the neural mechanisms involved in the modulatory effects of the periaqueductal gray (PAG) and its associated loops on SIH development hav
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Identification and validation of anoikis-related genes to clarify the prognosis and immune mechanisms of patients with low-grade glioma Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-05 Ji Wang, Yantin Liu, Min Qian, Wen Xu, Jianfeng Chen, Yuanguo Deng, Ran Luo, Jian Chen, Wenxue Jia, Longhu Liu, Chunlei Tian
Low-grade glioma (LGG) has an extremely poor prognosis, and the mechanism leading to malignant development has not been determined. The aim of our study was to clarify the function and mechanism of anoikis and TIMP1 in the malignant progression of LGG. We screened 7 anoikis-related genes from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases to construct a prognostic-predicting
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DNA binding reveals hidden interdomain allostery of a MazE antitoxin from Mycobacterium tuberculosis Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-05 Hyun-Jong Eun, Soo-Yeon Lee, Ki-Young Lee
Type II toxin−antitoxin (TA) systems are ubiquitously distributed genetic elements in prokaryotes and are crucial for cell maintenance and survival under environmental stresses. The antitoxin is a modular protein consisting of the disordered C-terminal region that physically contacts and neutralizes the cognate toxin and the well-folded N-terminal DNA binding domain responsible for autorepression of
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Neutrophil extracellular traps as a unique target in the treatment of inflammatory pain Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-05 Wanxiang Qin, Yuping Li, Jian Cui, Bao Yu, Lehua Yu, Congwen Yang
Pain is a widespread motivation for seeking healthcare and stands as a substantial global public health concern. Despite comprehensive investigations into the mechanisms of pain sensitization induced by inflammation, efficacious treatments options remain scarce Neutrophil extracellular traps (NETs) have been associated with the progression and tissue damage of diverse inflammatory diseases. This study
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Delivery of miR-29a improves the permeability of cisplatin by downregulating collagen I expression Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-05 Zhongyu Qin, Jin Ma, Xu Chu, Taozhen Guo, Pengcheng Feng, Ming Wang
In the clinical setting, chemotherapy is the most widely used antitumor treatment, however, chemotherapy resistance significantly limits its efficacy. Reduced drug influx is a key mechanism of chemoresistance, and inhibition of the complexity of the tumor microenvironment (TME) may improve chemotherapy drug influx and therapeutic efficiency. In the current study, we identified that the major extracellular
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Intermittent administration of PTH for the treatment of inflammatory bone loss does not enhance entheseal pathological new bone formation Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-04 Shuai Zhang, Wenjun Hao, Dongying Chen, Siwen Chen, Zihao Li, Fangling Zhong, Haitao Wang, Jianru Wang, Zhaomin Zheng, Zhongping Zhan, Guo Dai, Hui Liu
To investigate the effect of intermittent parathyroid hormone (iPTH) administration on pathological new bone formation during treatment of ankylosing spondylitis-related osteoporosis. Animal models with pathological bone formation caused by hypothetical AS pathogenesis received treatment with iPTH. We determined the effects of iPTH on bone loss and the formation of pathological new bone with micro-computed
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Non-cytotoxic nanomolar concentration of arctigenin protects neuronal cells from chemotherapy-induced ferroptosis by regulating SLC7A11-cystine-cysteine axis Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-04 Zhuoqun Li, Lixing Cao, Kai Han, Lihong Fan, Chong Zhao, Shutao Yin, Hongbo Hu
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Plasmid expression of Deinococcus radiodurans RecA confers UV-A protection to Escherichia coli with an inverse protein dose dependence, which does not exceed conspecific RecA protection Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-04 Olga A. Chinchilla, Vince J. LiCata
Low level expression in of the RecA protein from the radiation resistant bacterium protects a RecA deficient strain of from UV-A irradiation by up to ∼160% over basal UV-A resistance. The protection effect is inverse protein dose dependent: increasing the expression level of the RecA (DrRecA) protein decreases the protection factor. This inverse protein dose dependence effect helps resolve previously
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Disease-modifying potential of diphenyl diselenide in an experimental osteoarthritis model Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-04 Li Qiao, Zhiyao Li, Peihua Shi
Oxidative stress is a key factor in the disruption of cartilage homeostasis during the development of osteoarthritis (OA). Organic selenium (Se)-containing compounds such as diselenides have excellent antioxidant activity and may prevent related diseases. We aimed to examine the benefits of the synthetic small molecule diphenyl diselenide (DPDSe) in OA models in vitro and in vivo. Our findings showed
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Cardiac-specific deletion of heat shock protein 60 induces mitochondrial stress and disrupts heart development in mice Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-04 Tao Shen, Shuting Wang, Can Huang, Siting Zhu, Xiangbin Zhu, Na Li, Hong Wang, Lei Huang, Mingming Ren, Zhen Han, Jianjun Ge, Ze'e Chen, Kunfu Ouyang
Congenital heart diseases are the most common birth defects around the world. Emerging evidence suggests that mitochondrial homeostasis is required for normal heart development. In mitochondria, a series of molecular chaperones including heat shock protein 60 (HSP60) are engaged in assisting the import and folding of mitochondrial proteins. However, it remains largely obscure whether and how these
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Crebinostat facilitates memory formation Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-04 Deepti Dama, Shiv K. Sharma
Protein modifications importantly contribute to memory formation. Protein acetylation is a post-translational modification of proteins that regulates memory formation. Acetylation level is determined by the relative activities of acetylases and deacetylases. Crebinostat is a histone deacetylase inhibitor. Here we show that in an object recognition task, crebinostat facilitates memory formation by a
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YabJ from Staphylococcus aureus entraps chlorides within its pocket Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-04 Cheolwoo Jeong, Hyo Jung Kim
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Application of dual chemotherapeutic drug delivery system based on metal-organic framework platform in enhancing tumor regression for breast cancer research Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-04 Lin Zhan, Xuelian Yin, Li Qiu, Chenchen Li, Yanli Wang
The nanomedicine system based on dual drug delivery systems (DDDs) can significantly enhance the efficacy of tumor treatment. Herein, a metal-organic framework, Zeolite imidazole salt frames 8 (ZIF-8), was successfully utilized as a carrier to load the dual chemotherapeutic drugs doxorubicin (DOX) and camptothecin (CPT), named DOX/CPT@ZIF-8 (denoted as DCZ), and their inhibitory effects on 4T1 breast
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SS-31 inhibits the inflammatory response by increasing ATG5 and promoting autophagy in lipopolysaccharide-stimulated HepG2 cells Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-04 Yunan Mo, Songyun Deng, Yuhang Ai, Wenchao Li
SS-31 is a mitochondria-targeting short peptide. Recent studies have indicated its hepatoprotective effects. In our study, we investigated the impact of SS-31 on LPS-induced autophagy in HepG2 cells. The results obtained from a dual-fluorescence autophagy detection system revealed that SS-31 promotes the formation of autolysosomes and autophagosomes, thereby facilitating autophagic flux to a certain
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Semaglutide alters gut microbiota and improves NAFLD in db/db mice Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-03 Tuohua Mao, Chenxuan Zhang, Shuang Yang, Yingying Bi, Man Li, Jia Yu
Non-alcoholic fatty liver disease (NAFLD) is the most common liver disease associated with type 2 diabetes mellitus (T2D). NAFLD can progress to nonalcoholic steatohepatitis (NASH), cirrhosis, and even cancer, all of which have a very poor prognosis. Semaglutide, a novel glucagon-like peptide-1 (GLP-1) receptor agonist, has been recognized as a specific drug for the treatment of diabetes. In this study
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Maackia amurensis seed lectin (MASL) and soluble human podoplanin (shPDPN) sequence analysis and effects on human oral squamous cell carcinoma (OSCC) cell migration and viability Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-03 Ariel C. Yin, Cayla J. Holdcraft, Eamonn J. Brace, Tyler J. Hellmig, Sayan Basu, Saumil Parikh, Katarzyna Jachimowska, Evelyne Kalyoussef, Dylan Roden, Soly Baredes, Eugenio M. Capitle, David I. Suster, Alan J. Shienbaum, Caifeng Zhao, Haiyan Zheng, Kevin Balcaen, Simon Devos, Jurgen Haustraete, Mahnaz Fatahzadeh, Gary S. Goldberg
lectins serve as research and botanical agents that bind to sialic residues on proteins. For example, seed lectin (MASL) targets the sialic acid modified podoplanin (PDPN) receptor to suppress arthritic chondrocyte inflammation, and inhibit tumor cell growth and motility. However, lectin nomenclature and composition are not clearly defined. Here, we sought to definitively characterize MASL and its
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Nafamostat mesylate decreases skin flap necrosis in a mouse model of type 2 diabetes by protecting the endothelial glycocalyx Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-03 Yohei Fukuda, Hideshi Okada, Hiroyuki Tomita, Kodai Suzuki, Kosuke Mori, Chihiro Takada, Yuki Kawasaki, Hirotsugu Fukuda, Toru Minamiyama, Ayane Nishio, Takuto Shimada, Ayumi Kuroda, Akihiro Uchida, Keiko Suzuki, Ryo Kamidani, Yuichiro Kitagawa, Tetsuya Fukuta, Takahito Miyake, Takahiro Yoshida, Akio Suzuki, Nobuyuki Tetsuka, Shozo Yoshida, Shinji Ogura
The success rate of flap tissue reconstruction has increased in recent years owing to advancements in microsurgical techniques. However, complications, such as necrosis, are still more prevalent in diabetic patients compared to non-diabetic individuals, presenting an ongoing challenge. To address this issue, many previous studies have examined vascular anastomoses dilation and stability, primarily
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Mdivi-1: Effective but complex mitochondrial fission inhibitor Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-03 Seor I Ahn, Sung Kyung Choi, Myoung Jun Kim, Jinhong Wie, Jueng Soo You
Mdivi-1, Mitochondrial DIVIsion inhibitor 1, has been widely employed in research under the assumption that it exclusively influences mitochondrial fusion, but effects other than mitochondrial dynamics have been underinvestigated. This paper provides transcriptome and DNA methylome-wide analysis for Mdivi-1 treated SH-SY5Y human neuroblastoma cells using RNA sequencing (RNA-seq) and methyl capture
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Indole-3-carboxaldehyde alleviates acetaminophen-induced liver injury via inhibition of oxidative stress and apoptosis Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-03 Xinlei Liu, Rui Liu, Yancheng Wang
Drug-induced liver injury (DILI) occurs frequently and can be life-threatening. Increasing researches suggest that acetaminophen (APAP) overdose is a leading cause of drug-induced liver injury. Indole-3-carboxaldehyde (I3A) alleviates hepatic inflammation, fibrosis and atherosclerosis, suggesting a potential role in different disease development. However, the question of whether and how I3A protects
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Hippuric acid alleviates dextran sulfate sodium-induced colitis via suppressing inflammatory activity and modulating gut microbiota Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-03 Yan Yang, Shiqin Huang, Yangjie Liao, Xing Wu, Chao Zhang, Xiaoyan Wang, Zhenyu Yang
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Strong evolutionary constraints against amino acid changes in the P2 subdomain of sapovirus GI.1 capsid protein VP1 Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-02 Masaru Yokoyama, Yen Hai Doan, Kazushi Motomura, Hironori Sato, Tomoichiro Oka
Sapovirus (SaV) is a nonenveloped RNA virus that causes acute gastroenteritis in humans. Although SaV is a clinically important pathogen in children, an effective vaccine is currently unavailable. The capsid protein VP1 of SaVs forms the outer shell of the virion and is highly diverse, as often seen in the virion-surface proteins of RNA viruses, creating an obstacle for vaccine development. We here
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Reduced OxyR positively regulates the prodigiosin biosynthesis in Serratia marcescens FS14 Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-02 Hong Liu, Fenglian Chu, Yi Wu, Xiaochen Gu, Tingting Ran, Weiwu Wang, Dongqing Xu
OxyR, a LysR family transcriptional regulator, plays vital roles in bacterial oxidative stress response. In this study, we found that the deletion of not only inhibited the antioxidant capacity of FS14, but also decreased the production of prodigiosin. Further study revealed that OxyR activated the prodigiosin biosynthesis at the transcriptional level. Complementary results showed that not only the
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Metabolic engineering of Candida tropicalis for efficient 1,2,4-butanetriol production Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-04-01 Jingyun Li, Yuanyuan Xia, Bo Wei, Wei Shen, Haiquan Yang, Xianzhong Chen
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Association mechanism between Arabidopsis immune coreceptor BAK1 and Pseudomonas syringae effector HopF2 Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-31 Rui Han, Tongtong Zhu, Lili Zhou, Meiqing Chen, Dongli Wang, Junfeng Liu
Brassinosteroid activated kinase 1 (BAK1) is a cell-surface coreceptor which plays multiple roles in innate immunity of plants. HopF2 is an effector secreted by the bacterial pathogen pv. tomato DC3000 into and suppresses host immune system through interaction with BAK1 as well as its downstream kinase MKK5. The association mechanism of HopF2 to BAK1 remains unclear, which prohibits our understanding
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The role of macrophage polarization in vascular calcification Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-31 Dan Li, Chu Fan, Xuepeng Li, Lin Zhao
Vascular calcification is an important factor in the high morbidity and mortality of Cardiovascular and cerebrovascular diseases. Vascular damage caused by calcification of the intima or media impairs the physiological function of the vascular wall. Inflammation is a central factor in the development of vascular calcification. Macrophages are the main inflammatory cells. Dynamic changes of macrophages
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Effects of LP533401 on vascular and bone calcification in hyperlipidemic mice Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-31 Stuti Pradhan, Andy Hon, Yuxuan Xia, Sophia Kalanski, Nora Safvati, Mimi Lu, Linda L. Demer, Yin Tintut
Peripheral serotonin levels are associated with cardiovascular disease risk. We previously found that serum serotonin levels are higher in hyperlipidemic mice than wild-type mice. Evidence also suggests that serotonin regulates biomineralization, in that serotonin treatment augments TNF-a-induced matrix calcification of aortic valve interstitial cells and that a selective inhibitor of peripheral serotonin
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Photobiomodulation inhibits neuronal firing in the superficial but not deep layer of a rat spinal dorsal horn Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-30 Daisuke Uta, Naoya Ishibashi, Shinichi Tao, Masahito Sawahata, Toshiaki Kume
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The effect of Schizophyllan on the differentiation of osteoclasts and osteoblasts Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-30 Soo-Ho Kim, Keun Ha Park, Jun Lee, Seoung Hoon Lee, Jeong-Hwa Baek
Schizophyllan (SPG), a β-glucan from , is recognized for its antioxidant, immunoregulatory, and anticancer activities. In this study, its effects on bone cells, particularly osteoclasts and osteoblasts, were examined. We demonstrated that SPG dose-dependently inhibited osteoclastogenesis and reduced gene expression associated with osteoclast differentiation. SPG also decreased bone resorption and F-actin
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Metformin alters dendrite development and synaptic plasticity in rat cortical neurons Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-30 Muhammet Oner, Pang-Ting Cheng, Hsin-Yi Wang, Mei-Chih Chen, Ho Lin
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Mesenchymal cells regulate enteric neural crest cell migration via RET-GFRA1b trans-signaling Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-30 Mari Morikawa, Hisayoshi Yoshizaki, Yoshitomo Yasui, Shoichi Nishida, Yutaka Saikawa, Miyuki Kohno, Hideaki Okajima
During early development, the enteric nervous system forms from the migration of enteric neural crest cells (ENCCs) from the foregut to the hindgut, where they undergo proliferation and differentiation facilitated by interactions with enteric mesenchymal cells (EMCs). This study investigates the impact on ENCC migration of EMC-ENCC communication mediated by GFRA1b expressed in EMCs. GFRA1-expressing
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Structural and biochemical analysis of penicillin-binding protein 2 from Campylobacter jejuni Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-30 Hong Joon Choi, Dong Uk Ki, Sung-il Yoon
Penicillin-binding protein 2 (PBP2) plays a key role in the formation of peptidoglycans in bacterial cell walls by crosslinking glycan chains through transpeptidase activity. PBP2 is also found in , a pathogenic bacterium that causes food-borne enteritis in humans. To elucidate the essential structural features of PBP2 (cjPBP2) that mediate its biological function, we determined the crystal structure
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Measurement of the rotational relaxation time of intracellular water in dried yeast and Jurkat cells by near infrared spectroscopy Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-29 Junkai Zhang, Ryo Shirakashi
Molecular mobility of intracellular water is a crucial parameter in the study of the mechanism of desiccation tolerance. As one of the parameters that reflecting molecular mobility, the viscosity of intracellular water has been found intimately related with the protection of the phospholipid membrane because it quantifies the diffusion ability of water and mass in the intracellular environment. In
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Automatic segmentation of lysosomes and analysis of intracellular pH with Radachlorin photosensitizer and FLIM Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-29 A.V. Belashov, A.A. Zhikhoreva, A.V. Salova, T.N. Belyaeva, I.K. Litvinov, E.S. Kornilova, I.V. Semenova, O.S. Vasyutinskii
We report application of the fluorescence lifetime imaging microscopy (FLIM) for analysis of distributions of intracellular acidity using a chlorin-e6 based photosensitizer Radachlorin. An almost two-fold increase of the photosensitizer fluorescence lifetime in alkaline microenvironments as compared to acidic ones allowed for clear distinguishing between acidic and alkaline intracellular structures
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Substrate stiffness regulates type II diabetic fibroblast phenotype and metabolic activity Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-29 Amelia Huffer, Tugba Ozdemir
In people living with diabetes, impaired wound healing is a major concern as the formation of ulcerated wounds can drastically reduce both the effectiveness of the healing process and the quality of life of the patient. The healing of dermal wounds in particular involves a patient's fibroblasts building up a strong extracellular matrix of mostly collagen I and collagen III fibers, which the cells of
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Torsion affects the calculation of DNA twisting number Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Huimin Yang, Xuguang Shi
The topological properties of DNA have long been a focal point in biophysics. In the 1970s, White proposed that the topology of closed DNA double helix follows White's formula: . However, there has been controversy in the calculation of DNA twisting number, partly due to discrepancies in the definition of torsion in differential geometry. In this paper, we delved into a detailed study of torsion, revealing
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Overcoming statin resistance in prostate cancer cells by targeting the 3-hydroxy-3-methylglutaryl-CoA-reductase Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Andy Göbel, Sophie Pählig, Anja Motz, Dorit Breining, Sofia Traikov, Lorenz C. Hofbauer, Tilman D. Rachner
Prostate cancer is the most prevalent malignancy in men. While diagnostic and therapeutic interventions have substantially improved in recent years, disease relapse, treatment resistance, and metastasis remain significant contributors to prostate cancer-related mortality. Therefore, novel therapeutic approaches are needed. Statins are inhibitors of the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR)
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ELISA-based highly sensitive assay system for the detection of endogenous NGLY1 activity Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Haruhiko Fujihira, Keiko Sato, Yuji Nishiuchi, Takefumi Murase, Yuka Matsuda, Yukiko Yoshida, Takayuki Kamei, Tadashi Suzuki
Cytosolic peptide:N-glycanase (NGLY1, PNGase) is an enzyme that cleaves -glycans from misfolded glycoproteins. In 2012, a human genetic disorder, NGLY1 deficiency, was first reported to be caused by mutations of the gene. Since then, there has been rapid progresses on NGLY1 biology, and gene therapy has been proposed as a promising therapeutic option for NGLY1 deficiency. While a plasma/urine biomarker
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Cryo-EM structure of P-glycoprotein bound to triple elacridar inhibitor molecules Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Norie Hamaguchi-Suzuki, Naruhiko Adachi, Toshio Moriya, Satoshi Yasuda, Masato Kawasaki, Kano Suzuki, Satoshi Ogasawara, Naohiko Anzai, Toshiya Senda, Takeshi Murata
P-glycoprotein (P-gp) is an ATP-binding cassette transporter known for its roles in expelling xenobiotic compounds from cells and contributing to cellular drug resistance through multidrug efflux. This mechanism is particularly problematic in cancer cells, where it diminishes the therapeutic efficacy of anticancer drugs. P-gp inhibitors, such as elacridar, have been developed to circumvent the decrease
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Tibial transverse transport induces mobilization of endothelial progenitor cells to accelerate angiogenesis and ulcer wound healing through the VEGFA/CXCL12 pathway Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Weiqing Tian, Bo Feng, Lan Zhang, Guangming Dai, Ligong Lin, Wei Jiang, Yongjun Wang
Tibial transverse transport (TTT) can promote the healing of chronic foot ulcers, but the specific cellular and molecular mechanisms by which TTT promotes wound healing remain unclear. New Zealand White rabbits were selected to induce foot ulcer models. The treatment included unilateral TTT surgery and bilateral TTT surgery. Observation of tissue neovascularization structure by HE staining and CD31
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Nutraceutically-enhanced oral delivery of vitamin D3 via Bio-SNEDDS: Demonstrating in vivo superiority over pediatric formulations Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Mohsin Kazi, Fahad Almarri, Ahmad Abdul-Wahhab Shahba, Ajaz Ahmad, Salem Albraiki, Fars Kaed Alanazi
Vitamin D3 (VD3) deficiency among children in Saudi Arabia remains a pressing concern due to its poor bioavailability and the limitations of current pediatric formulations. To address this challenge, we developed a groundbreaking pediatric self-nanoemulsifying drug delivery system (Bio-SNEDDS) for VD3, fortified with black seed oil and moringa seed oil for dual therapeutic benefits. Through meticulous
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Ghrelin regulates the endoplasmic reticulum stress signalling pathway in gestational diabetes mellitus Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Xinying Li, Qun Ji, Chunrong Zhong, Congyin Wu, Jinchan Wu, Caihong Yuan, Jianmin Ran
We aimed to investigate the effects and mechanisms of the ghrelin-regulated endoplasmic reticulum stress (ERS) signalling pathway in gestational diabetes mellitus (GDM). Pregnant female C57BL/6 mice were randomly divided into a normal group, GDM group (high-fat diet + STZ), GDM + ghrelin group (acyl ghrelin), and GDM + ghrelin + ghrelin inhibitor group ([D-lys3]-GHRP-6). We measured body weight, the
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Characteristics of NAC transcription factors in Solanaceae crops and their roles in responding to abiotic and biotic stresses Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Xiaogang Ou, Lixinyu Sun, Yu Chen, Zhengwu Zhao, Wei Jian
As one of the largest transcription factor (TF) families in plants, the NAC (NAM, ATAF1/2, and CUC2) family plays important roles in response pathways to various abiotic and biotic stresses, such as drought, high salinity, low temperature, and pathogen infection. Although, there are a number of reviews on the involvement of NAC TF in plant responses to biotic and abiotic stresses, most of them are
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Transcriptomics-based identification of TYROBP and TLR8 as novel macrophage-related biomarkers for the diagnosis of acute rejection after kidney transplantation Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Jun Pei, Jie Zhang, Chengjun Yu, Jin Luo, Sheng Wen, Yi Hua, Guanghui Wei
Macrophages play an important role in the development and progression of acute rejection after kidney transplantation. The study aims to investigate the biological role and significance of macrophage-associated genes (MAG) in acute rejection after kidney transplantation. We utilized transcriptome sequencing results from public databases related to acute rejection of kidney transplantation for comprehensive
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Transcriptomics confirms IRF1 as a key regulator of pyroptosis in diabetic retinopathy Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-28 Yang Xian, Xingli Wang, Yong Yu, XiaoLong Chen
Diabetic retinopathy (DR) is a retinal microvascular complication caused by hyperglycemia, which can lead to visual impairment or blindness. Pyroptosis is a type of inflammation-related programmed cell death, activated by caspase-1, resulting in the maturation of IL-1β and IL-18 and the rupture of the cell membrane. RNA sequencing (RNA-seq) is a high-throughput sequencing technique that reveals the
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Functional insights of Tyr37 in framework region 2 directly contributing to the binding affinities and dissociation kinetics in single-domain VHH antibodies Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-27 Koichi Yamamoto, Satoru Nagatoishi, Makoto Nakakido, Daisuke Kuroda, Kouhei Tsumoto
Single-domain VH antibody is regarded as one of the promising antibody classes for therapeutic and diagnostic applications. VH antibodies have amino acids in framework region 2 that are distinct from those in conventional antibodies, such as the Val37Phe/Tyr (V37F/Y) substitution. Correlations between the residue type at position 37 and the conformation of the CDR3 in VH antigen recognition have been
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Dnttip2 is essential for 18S rRNA processing and digestive organ development in zebrafish Biochem. Biophys. Res. Commun. (IF 3.1) Pub Date : 2024-03-27 Ru Zhou, Wei Cui, Ni Zeng, Baochun Su, Yayue Chen, Hui Shi, Shuyi Zhao
Dnttip2 is one of the components of the small subunit (SSU) processome. In yeast, depletion of leads to an inefficient processing of pre-rRNA and a decrease in synthesis of the mature 18S rRNA. However, the biological roles of Dnttip2 in higher organisms are poorly defined. In this study, we demonstrate that is a maternal gene in zebrafish. Depletion of Dnttip2 leads to embryonic lethal with severe