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Current application and future perspectives of antimicrobial degradable bone substitutes for chronic osteomyelitis Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-27 Chenxi Jiang, Guangxun Zhu, Qian Liu
Chronic osteomyelitis remains a persistent challenge for the surgeons due to its refractory nature. Generally, treatment involves extensive debridement of necrotic bone, filling of dead space, adequate antimicrobial therapy, bone reconstruction, and rehabilitation. However, the optimal choice of bone substitute to manage the bone defect remains debatable. This paper reviewed the clinical evidence for
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Periodic static compression of micro-strain pattern regulates endochondral bone formation Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-27 Pengzhen Cheng, Xueyi Zhao, Meige Han, Yaping Zhuang, Fenru Ning, Yaqian Hu, Weiguang Lu, Sheng Miao, Chengxiang Zhao, Liyuan Jia, Xue Hao, Meng Sun, Junxiang Wang, Fulin Chen, Liu Yang, Qiang Jie
Introduction: Developmental engineering based on endochondral ossification has been proposed as a potential strategy for repairing of critical bone defects. Bone development is driven by growth plate-mediated endochondral ossification. Under physiological conditions, growth plate chondrocytes undergo compressive forces characterized by micro-mechanics, but the regulatory effect of micro-mechanical
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A review of the current state of natural biomaterials in wound healing applications Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-27 Mojtaba Ansari, Ahmad Darvishi
Skin, the largest biological organ, consists of three main parts: the epidermis, dermis, and subcutaneous tissue. Wounds are abnormal wounds in various forms, such as lacerations, burns, chronic wounds, diabetic wounds, acute wounds, and fractures. The wound healing process is dynamic, complex, and lengthy in four stages involving cells, macrophages, and growth factors. Wound dressing refers to a substance
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The relationship between lateral femoral condyle ratio measured by MRI and anterior cruciate ligament injury Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-27 Yang Sun, Yun Tang
Background:Previous studies have shown that the lateral femoral condyle ratio (LFCR) measured by X-ray has a significant relationship with the anterior cruciate ligament (ACL) injury. However, few relevant studies have been performed on LFCR measured by magnetic resonance imaging (MRI).Purpose:(1) To evaluate the relationship between LFCR measured by MRI and ACL injury or rerupture. (2) To compare
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Stress reduction through cortical bone thickening improves bone mechanical behavior in adult female Beclin-1+/− mice Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-27 Jiaojiao Yang, Qilin Pei, Xingfan Wu, Xin Dai, Xi Li, Jun Pan, Bin Wang
Fragility fractures, which are more prevalent in women, may be significantly influenced by autophagy due to altered bone turnover. As an essential mediator of autophagy, Beclin-1 modulates bone homeostasis by regulating osteoclast and chondrocyte differentiation, however, the alteration in the local bone mechanical environment in female Beclin-1+/− mice remains unclear. In this study, our aim is to
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Optimization of a tunable process for rapid production of calcium phosphate microparticles using a droplet-based microfluidic platform Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-27 Y. Alaoui Selsouli, H. S. Rho, M. Eischen-Loges, V. P. Galván-Chacón, C. Stähli, Y. Viecelli, N. Döbelin, M. Bohner, Z. Tahmasebi Birgani, P. Habibović
Calcium phosphate (CaP) biomaterials are amongst the most widely used synthetic bone graft substitutes, owing to their chemical similarities to the mineral part of bone matrix and off-the-shelf availability. However, their ability to regenerate bone in critical-sized bone defects has remained inferior to the gold standard autologous bone. Hence, there is a need for methods that can be employed to efficiently
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Paraguay’s approach to biotechnology governance: a comprehensive guide Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-27 Nidia Benítez Candia, María Gabriela Ulke Mayans, Pablo Hernán Sotelo, Eva Nara Pereira, Andrea Alejandra Arrúa Alvarenga, Danilo Fernández Ríos
This study analyzes Paraguay’s biotechnology regulatory framework and its alignment with international standards amid biotechnological advancements. It also identifies areas of improvement for enhancing framework effectiveness. Through this work, we aim to provide a resource for policymakers, stakeholders, and researchers navigating Paraguay’s biotechnology regulation.
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Advancements in CHO metabolomics: techniques, current state and evolving methodologies Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-27 Rita Singh, Eram Fatima, Lovnish Thakur, Sevaram Singh, Chandra Ratan, Niraj Kumar
Background: Investigating the metabolic behaviour of different cellular phenotypes, i.e., good/bad grower and/or producer, in production culture is important to identify the key metabolite(s)/pathway(s) that regulate cell growth and/or recombinant protein production to improve the overall yield. Currently, LC-MS, GC-MS and NMR are the most used and advanced technologies for investigating the metabolome
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Analyzing isolated degeneration of lumbar facet joints: implications for degenerative instability and lumbar biomechanics using finite element analysis Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-27 Jun Sung Park, Tae Sik Goh, Jung Sub Lee, Chiseung Lee
The facet joint contributes to lumbar spine stability as it supports the weight of body along with the intervertebral discs. However, most studies on the causes of degenerative lumbar diseases focus on the intervertebral discs and often overlook the facet joints. This study aimed to investigate the impact of facet joint degeneration on the degenerative changes and diseases of the lumbar spine. A finite
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Shape or size matters? Towards standard reporting of tensile testing parameters for human soft tissues: systematic review and finite element analysis Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-26 Alvin C. Lin, Felix Pirrung, Justyna A. Niestrawska, Benjamin Ondruschka, Gerald Pinter, Petr Henyš, Niels Hammer
Material properties of soft-tissue samples are often derived through uniaxial tensile testing. For engineering materials, testing parameters (e.g., sample geometries and clamping conditions) are described by international standards; for biological tissues, such standards do not exist. To investigate what testing parameters have been reported for tensile testing of human soft-tissue samples, a systematic
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Magnetically actuated sonodynamic nanorobot collectives for potentiated ovarian cancer therapy Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-26 Yixuan Zhou, Ziqi Cao, Lixian Jiang, Ying Chen, Xiaoyu Cui, Jianrong Wu, Xue Xie, Longchen Wang, Tao Ying
Ovarian cancer presents a substantial challenge due to its high mortality and recurrence rates among gynecological tumors. Existing clinical chemotherapy treatments are notably limited by drug resistance and systemic toxic side effects caused by off target drugs. Sonodynamic therapy (SDT) has emerged as a promising approach in cancer treatment, motivating researchers to explore synergistic combinations
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Genomic and metabolic instability during long-term fermentation of an industrial Saccharomyces cerevisiae strain engineered for C5 sugar utilization Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-26 Maëlle Duperray, Mathéo Delvenne, Jean Marie François, Frank Delvigne, Jean-Pascal Capp
The genetic stability and metabolic robustness of production strains is one of the key criteria for the production of bio-based products by microbial fermentation on an industrial scale. These criteria were here explored in an industrial ethanol-producer strain of Saccharomyces cerevisiae able to co-ferment D-xylose and L-arabinose with glucose through the chromosomal integration of several copies
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The virtual pivot point concept improves predictions of ground reaction forces Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-26 Heiko Wagner, Oliver Schmitz, Kim J. Boström
Ground reaction forces (GRFs) are essential for the analysis of human movement. To measure GRFs, 3D force plates that are fixed to the floor are used with large measuring ranges, excellent accuracy and high sample frequency. For less dynamic movements, like walking or squatting, portable 3D force plates are used, while if just the vertical component of the GRFs is of interest, pressure plates or in-shoe
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Towards optimized tissue regeneration: a new 3D printable bioink of alginate/cellulose hydrogel loaded with thrombocyte concentrate Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-26 Till Grandjean, Natarajan Perumal, Caroline Manicam, Björn Matthey, Tao Wu, Daniel G. E. Thiem, Stefan Stein, Dirk Henrich, Peer W. Kämmerer, Bilal Al-Nawas, Ulrike Ritz, Sebastian Blatt
Introduction:Autologous platelet concentrate (APC) are pro-angiogenic and can promote wound healing and tissue repair, also in combination with other biomaterials. However, challenging defect situations remain demanding. 3D bioprinting of an APC based bioink encapsulated in a hydrogel could overcome this limitation with enhanced physio-mechanical interface, growth factor retention/secretion and defect-personalized
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Utilizing GO/PEDOT:PSS/PtNPs-enhanced high-stability microe-lectrode arrays for investigating epilepsy-induced striatal electrophysiology alterations Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-25 Meiqi Han, Yu Wang, Luyi Jing, Gucheng Yang, Yaoyao Liu, Fan Mo, Zhaojie Xu, Jinping Luo, Qianli Jia, Yuxin Zhu, Hanwen Cao, Xinxia Cai, Juntao Liu
The striatum plays a crucial role in studying epilepsy, as it is involved in seizure generation and modulation of brain activity. To explore the complex interplay between the striatum and epilepsy, we engineered advanced microelectrode arrays (MEAs) specifically designed for precise monitoring of striatal electrophysiological activities in rats. These observations were made during and following seizure
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GelMA synthesis and sources comparison for 3D multimaterial bioprinting Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-25 Cesare Gabriele Gaglio, Désireé Baruffaldi, Candido Fabrizio Pirri, Lucia Napione, Francesca Frascella
Gelatin Methacryloyl (GelMA) is one of the most used biomaterials for a wide range of applications, such as drug delivery, disease modeling and tissue regeneration. GelMA is obtained from gelatin, which can be derived from different sources (e.g., bovine skin, and porcine skin), through substitution of reactive amine and hydroxyl groups with methacrylic anhydride (MAA). The degree of functionalization
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Reconstruction of the genome-scale metabolic network model of Sinorhizobium fredii CCBAU45436 for free-living and symbiotic states Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-25 Anqiang Ye, Jian-Ning Shen, Yong Li, Xiang Lian, Bin-Guang Ma, Feng-Biao Guo
Sinorhizobium fredii CCBAU45436 is an excellent rhizobium that plays an important role in agricultural production. However, there still needs more comprehensive understanding of the metabolic system of S. fredii CCBAU45436, which hinders its application in agriculture. Therefore, based on the first-generation metabolic model iCC541 we developed a new genome-scale metabolic model iAQY970, which contains
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CRISPR-HOLMES-based NAD+ detection Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-25 Songkuan Zhuang, Tianshuai Hu, Hongzhong Zhou, Shiping He, Jie Li, Yuehui Zhang, Dayong Gu, Yong Xu, Yijian Chen, Jin Wang
Studies have indicated that the intracellular nicotinamide adenine dinucleotide (NAD+) level is associated with the occurrence and development of many diseases. However, traditional nicotinamide adenine dinucleotide (NAD+) detection techniques are time-consuming and may require large and expensive instruments. We recently found that the clustered regularly interspaced short palindromic repeat (CRISPR)-Cas12a
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Systematic investigation of TetR-family transcriptional regulators and their roles on lignocellulosic inhibitor acetate tolerance in Zymomonas mobilis Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-22 Yubei Xiao, Tongjia Qin, Shuche He, Yunhao Chen, Han Li, Qiaoning He, Xia Wang, Shihui Yang
TetR-family transcriptional regulators are widely distributed among bacteria and involved in various cellular processes such as multidrug and inhibitor resistance. Zymomonas mobilis is a industrial bacterium for lignocellulosic ethanol production. Although TetR-family regulators and their associated RND-family efflux pumps in Z. mobilis have been identified to be differentially expressed under various
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Improving product quality and productivity of an antibody-based biotherapeutic using inverted frustoconical shaking bioreactors Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-22 Xuekun Wang, Jin Xu, Qingcheng Guo, Zhenhua Li, Jiawei Cao, Rongrong Fu, Mengjiao Xu, Xiang Zhao, Fugui Wang, Xinmeng Zhang, Taimin Dong, Xu Li, Weizhu Qian, Shen Hou, Lusha Ji, Dapeng Zhang, Huaizu Guo
The Chinese hamster ovarian (CHO) cells serve as a common choice in biopharmaceutical production, traditionally cultivated in stirred tank bioreactors (STRs). Nevertheless, the pursuit of improved protein quality and production output for commercial purposes demand exploration into new bioreactor types. In this context, inverted frustoconical shaking bioreactors (IFSB) present unique physical properties
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Numerical analysis of MICP treated sand based on bio-chemo-hydro model Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-22 Delong Li, Shengzhe Chen, Xing Gao
Microbially Induced Calcite Precipitation (MICP) represents an environmentally friendly and innovative soil grouting technology. Involving intricate biochemical processes, it poses challenges for a thorough investigation of factors influencing microbial grouting effectiveness through experimentation alone. Consequently, A three-dimensional numerical model was developed to predict the permeability of
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Active osseointegration in an ex vivo porcine bone model Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-22 Thomas A. G. Hall, Konstantinos Theodoridis, Nupur Kohli, Frederic Cegla, Richard J. van Arkel
Achieving osseointegration is a fundamental requirement for many orthopaedic, oral, and craniofacial implants. Osseointegration typically takes three to 6 months, during which time implants are at risk of loosening. The aim of this study was to investigate whether osseointegration could be actively enhanced by delivering controllable electromechanical stimuli to the periprosthetic bone. First, the
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Design of a simplified cranial substitute with a modal behavior close to that of a human skull Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-22 Natacha Elster, Johanna Boutillier, Nicolas Bourdet, Pascal Magnan, Pierre Naz, Rémy Willinger, Caroline Deck
Individuals exposed to the propagation of shock waves generated by the detonation of explosive charges may suffer Traumatic Brain Injury. The mechanism of cranial deflection is one of many hypotheses that could explain the observed brain damage. To investigate this physical phenomenon in a reproducible manner, a new simplified cranial substitute was designed with a mechanical response close to that
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Effects of non-invasive cervical spinal cord neuromodulation by trans-spinal electrical stimulation on cortico-muscular descending patterns in upper extremity of chronic stroke Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-21 Jianing Zhang, Maner Wang, Monzurul Alam, Yong-Ping Zheng, Fuqiang Ye, Xiaoling Hu
Background: Trans-spinal electrical stimulation (tsES) to the intact spinal cord poststroke may modulate the cortico-muscular control in stroke survivors with diverse lesions in the brain. This work aimed to investigate the immediate effects of tsES on the cortico-muscular descending patterns during voluntary upper extremity (UE) muscle contractions by analyzing cortico-muscular coherence (CMCoh) and
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Reliable in vitro method for the evaluation of the primary stability and load transfer of transfemoral prostheses for osseointegrated implantation Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-21 Giulia Galteri, Marco Palanca, Domenico Alesi, Stefano Zaffagnini, Kavin Morellato, Emanuele Gruppioni, Luca Cristofolini
Osseointegrated transfemoral prostheses experience aseptic complications with an incidence between 3% and 30%. The main aseptic risks are implant loosening, adverse bone remodeling, and post-operative periprosthetic fractures. Implant loosening can either be due to a lack of initial (primary) stability of the implant, which hinders bone ingrowth and therefore prevents secondary stability, or, in the
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A study on the ultimate mechanical properties of middle-aged and elderly human aorta based on uniaxial tensile test Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-21 Hongbing Chen, Minzhu Zhao, Yongguo Li, Qi Wang, Yu Xing, Cunhao Bian, Jianbo Li
Background:The mechanical properties of the aorta are particularly important in clinical medicine and forensic science, serving as basic data for further exploration of aortic disease or injury mechanisms.Objective:To study the influence of various factors (age, gender, test direction, anatomical location, and pathological characteristics) on the mechanical properties and thickness of the aorta.Methods:In
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Novel bioactive extract from yarrow obtained by the supercritical antisolvent-assisted technique inhibits lipid metabolism in colorectal cancer Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-21 Marta Gómez de Cedrón, Maria de las Nieves Siles-Sanchez, Diego Martín-Hernandez, Laura Jaime, Susana Santoyo, Ana Ramírez de Molina
Background: Altered lipid metabolism in cancer is associated to dissemination and prognosis. Bioactive compounds naturally occurring in Achillea millefolium L. (yarrow) have been reported to exert antitumour activities. Food biotechnology may provide on-demand mixtures of bioactive compounds with complementary activities in cancer treatment.Methods: Supercritical-antisolvent-precipitation (SAS) has
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Sensory neuron transient receptor potential vanilloid-1 channel regulates angiogenesis through CGRP in vivo Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-21 Zhanfeng Zhu, Yixuan Jiang, Zixia Li, Yu Du, Qinyi Chen, Qiang Guo, Yu Ban, Ping Gong
Angiogenesis plays a key role in bone regeneration. The role of neurons of peripheral nerves involved in angiogenesis of bone defects needs to be explored. The transient receptor potential vanilloid 1 (TRPV1), a nociceptor of noxious stimuli, is expressed on sensory neurons. Apart from nociception, little is known about the role of sensory innervation in angiogenesis. Calcitonin gene-related peptide
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Development of high-efficiency superparamagnetic drug delivery system with MPI imaging capability Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-20 Shi Bai, Xiao-dan Zhang, Yu-qi Zou, Yu-xi Lin, Zhi-yao Liu, Ke-wen Li, Ping Huang, Takashi Yoshida, Yi-li Liu, Ming-shan Li, Wei Zhang, Xiao-ju Wang, Min Zhang, Cheng Du
In this study, a high-efficiency superparamagnetic drug delivery system was developed for preclinical treatment of bladder cancer in small animals. Two types of nanoparticles with magnetic particle imaging (MPI) capability, i.e., single- and multi-core superparamagnetic iron oxide nanoparticles (SPIONs), were selected and coupled with bladder anti-tumor drugs by a covalent coupling scheme. Owing to
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Effects of 12-week gait retraining on plantar flexion torque, architecture, and behavior of the medial gastrocnemius in vivo Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-20 Chuyi Zhang, Liqin Deng, Xini Zhang, Kaicheng Wu, Jianglong Zhan, Weijie Fu, Jing Jin
Objective:This study aims to explore the effects of 12-week gait retraining (GR) on plantar flexion torque, architecture, and behavior of the medial gastrocnemius (MG) during maximal voluntary isometric contraction (MVIC).Methods:Thirty healthy male rearfoot strikers were randomly assigned to the GR group (n = 15) and the control (CON) group (n = 15). The GR group was instructed to wear minimalist
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Construction of an Escherichia coli chassis for efficient biosynthesis of human-like N-linked glycoproteins Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-20 Zixin Bao, Yuting Gao, Yitong Song, Ning Ding, Wei Li, Qiong Wu, Xiaomei Zhang, Yang Zheng, Junming Li, Xuejun Hu
The production of N-linked glycoproteins in genetically engineered Escherichia coli holds significant potential for reducing costs, streamlining bioprocesses, and enhancing customization. However, the construction of a stable and low-cost microbial cell factory for the efficient production of humanized N-glycosylated recombinant proteins remains a formidable challenge. In this study, we developed a
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Exosomes derived from MSC as drug system in osteoarthritis therapy Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-20 Shuzhan Wen, Xin Huang, Jingchun Ma, Guanglei Zhao, Tiancong Ma, Kangming Chen, Gangyong Huang, Jie Chen, Jingsheng Shi, Siqun Wang
Osteoarthritis (OA) is the most common degenerative disease of the joint with irreversible cartilage damage as the main pathological feature. With the development of regenerative medicine, mesenchymal stem cells (MSCs) have been found to have strong therapeutic potential. However, intraarticular MSCs injection therapy is limited by economic costs and ethics. Exosomes derived from MSC (MSC-Exos), as
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The mechanism of solid acid-catalyzed bamboo sawdust liquefaction under polyol systems Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-20 Bin Wu, Hongwei Tang, Yijia Huang, Mengke Zhao, Long Liang, Zhanghong Xie, Linshan Wei, Guigan Fang, Ting Wu
Solid acid catalysts are widely used in the field of biomass catalytic conversion owing to their advantages of low environmental pollution, easy separation and reusability. Nevertheless, there are relatively few studies on the mechanism of solid acid liquefaction for biomass. In this study, the effect of acid strength and acid amount of various solid acids on the liquefaction efficiency has been investigated
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Recent development and future application of biodegradable ureteral stents Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-20 Ke Hu, Zhipeng Hou, Yuanbin Huang, Xueying Li, Xiancheng Li, Liqun Yang
Ureteral stenting is a common clinical procedure for the treatment of upper urinary tract disorders, including conditions such as urinary tract infections, tumors, stones, and inflammation. Maintaining normal renal function by preventing and treating ureteral obstruction is the primary goal of this procedure. However, the use of ureteral stents is associated with adverse effects, including surface
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3D gait analysis in children using wearable sensors: feasibility of predicting joint kinematics and kinetics with personalized machine learning models and inertial measurement units Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-20 Shima Mohammadi Moghadam, Pablo Ortega Auriol, Ted Yeung, Julie Choisne
Introduction: Children’s walking patterns evolve with age, exhibiting less repetitiveness at a young age and more variability than adults. Three-dimensional gait analysis (3DGA) is crucial for understanding and treating lower limb movement disorders in children, traditionally performed using Optical Motion Capture (OMC). Inertial Measurement Units (IMUs) offer a cost-effective alternative to OMC, although
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Cultivation of microalgae in food processing effluent for pollution attenuation and astaxanthin production: a review of technological innovation and downstream application Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-20 Xiaowei Zhang, Qian Lu
Valorization of food processing effluent (FPE) by microalgae cultivation for astaxanthin production is regarded as a potential strategy to solve the environmental pollution of food processing industry and promote the development of eco-friendly agriculture. In this review paper, microalgal species which have the potential to be employed for astaxanthin in FPE were identified. Additionally, in terms
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The associations between lumbar proprioception and postural control during and after calf vibration in people with and without chronic low back pain Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-20 Zengming Hao, Xue Cheng, Haimei Jiang, Jiajia Yang, Yan Li, Wai Leung Ambrose Lo, Qiuhua Yu, Chuhuai Wang
The relationships of lumbar proprioception with postural control have not been clarified in people with chronic low back pain. This study aimed to compare the associations between lumbar proprioception and postural control in response to calf vibration in individuals with and without chronic low back pain. In this study, we recruited twenty patients with chronic low back pain (CLBP group) and twenty
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Codon-optimization in gene therapy: promises, prospects and challenges Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-19 Anastasiia Iu Paremskaia, Anna A. Kogan, Anastasiia Murashkina, Daria A. Naumova, Anakha Satish, Ivan S. Abramov, Sofya G. Feoktistova, Olga N. Mityaeva, Andrei A. Deviatkin, Pavel Yu Volchkov
Codon optimization has evolved to enhance protein expression efficiency by exploiting the genetic code’s redundancy, allowing for multiple codon options for a single amino acid. Initially observed in E. coli, optimal codon usage correlates with high gene expression, which has propelled applications expanding from basic research to biopharmaceuticals and vaccine development. The method is especially
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Differences in kinetic factors affecting gait speed between lesion sides in patients with stroke Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-19 Yusuke Sekiguchi, Dai Owaki, Keita Honda, Shin-Ichi Izumi, Satoru Ebihara
The differences in kinetic mechanisms of decreased gait speed across brain lesion sides have not been elucidated, including the arrangement of motor modules reflected by kinetic interjoint coordination. The purpose of this study was to elucidate the differences in the kinetic factors of slow gait speed in patients with stroke on the lesion sides. A three-dimensional motion analysis system was employed
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Multicriteria GIS-based assessment of biomass energy potentials in Nigeria Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-19 M. O. Ukoba, E. O. Diemuodeke, T. A. Briggs, M. M. Ojapah, K. E. Okedu, K. Owebor, K. Akhtar, C. Ilhami
The understanding of the geographical variability of biomass energy is an essential requirement for the optimal location of biomass energy conversion plants. This research presents a multicriteria GIS-based assessment of biomass energy potentials and the appropriate siting of biomass plants in Nigeria. The study applies the weighted overlay multicriteria decision analysis method. Crop and forest areas
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Natural based hydrogels promote chondrogenic differentiation of human mesenchymal stem cells Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-19 Tina Zahedi Tehrani, Shiva Irani, Abdolreza Ardeshirylajimi, Ehsan Seyedjafari
Background: The cartilage tissue lacks blood vessels, which is composed of chondrocytes and ECM. Due to this vessel-less structure, it is difficult to repair cartilage tissue damages. One of the new methods to repair cartilage damage is to use tissue engineering. In the present study, it was attempted to simulate a three-dimensional environment similar to the natural ECM of cartilage tissue by using
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Screening, identification, and mechanism analysis of starch-degrading bacteria during curing process in tobacco leaf Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-19 Yan Zhang, Chuandong Jiang, Yangyang Li, Jingguo Sun, Zhenguo Chen, Qiang Zhang, Guangwei Sun
Tobacco, a vital economic crop, had its quality post-curing significantly influenced by starch content. Nonetheless, the existing process parameters during curing were inadequate to satisfy the starch degradation requirements. Microorganisms exhibit inherent advantages in starch degradation, offering significant potential in the tobacco curing process. Our study concentrated on the microbial populations
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Advances in succinic acid production: the enhancement of CO2 fixation for the carbon sequestration benefits Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Fanzhen Lin, Wenwei Li, Dan Wang, Ge Hu, Zhao Qin, Xue Xia, Lin Hu, Xuemei Liu, Ruoshi Luo
Succinic acid (SA), one of the 12 top platform chemicals produced from biomass, is a precursor of various high value-added derivatives. Specially, 1 mol CO2 is assimilated in 1 mol SA biosynthetic route under anaerobic conditions, which helps to achieve carbon reduction goals. In this review, methods for enhanced CO2 fixation in SA production and utilization of waste biomass for SA production are reviewed
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Photothermal hydrogels for infection control and tissue regeneration Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Siyu Sun, Guangyang Jiang, Jianru Dong, Xi Xie, Jinfeng Liao, Yongqiang Tian
In this review, we report investigating photothermal hydrogels, innovative biomedical materials designed for infection control and tissue regeneration. These hydrogels exhibit responsiveness to near-infrared (NIR) stimulation, altering their structure and properties, which is pivotal for medical applications. Photothermal hydrogels have emerged as a significant advancement in medical materials, harnessing
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Revolutionizing nephrology research: expanding horizons with kidney-on-a-chip and beyond Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Wei Huang, Yi-Yuan Chen, Fang-Fang He, Chun Zhang
Organs-on-a-chip (OoC) is a microengineered three-dimensional cell culture system developed for decades. Utilizing microfluidic technology, OoC cultivates cells on perfusable channels to construct in vitro organ models, enabling the simulation of organ-level functions under physiological and pathophysiological conditions. The superior simulation capabilities compared to traditional animal experiments
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Knowledge and attitude toward transcranial magnetic stimulation among rehabilitation specialists in Saudi Arabia Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Alaa M. Albishi, Saja A. Alhadlaq, Rahaf T. Altowairqi, Mohammed F. Alharbi, Abdulrahman M. Alsubiheen, Manal H. Alosaimi, Shahid Bashir, Ahmad O. Alokaily
Research has demonstrated the benefits of transcranial magnetic stimulation (TMS) in rehabilitation. TMS has been widely used in clinical and research settings for individuals with and without neurological dysfunctions. Therefore, understanding the knowledge and attitudes of rehabilitation specialists regarding TMS is crucial for its application. To our knowledge, no such studies have previously been
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Site-selective fatty acid chain conjugation of the N-terminus of the recombinant human granulocyte colony-stimulating factor Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Xu-Dong Wang, Zhi-Hao Su, Jie Du, Wei-Jia Yu, Wen-Long Sun
The clinical application of the recombinant human granulocyte colony-stimulating factor (rhG-CSF) is restricted by its short serum half-life. Herein, site-selective modification of the N-terminus of rhG-CSF with PAL-PEG3-Ph-CHO was used to develop a long-acting rhG-CSF. The optimized conditions for rhG-CSF modification with PAL-PEG3-Ph-CHO were: reaction solvent system of 3% (w/v) Tween 20 and 30 mM
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Simulation and analysis of chest loads on crews during Lunar-Earth re-entry returns Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Jiatao Wang, Zhiyong Peng, Yongjie Yao, Qianxiang Zhou, Pan Guo
The safety of crews is the primary concern in the manned lunar landing project, particularly during re-entry as the manned spacecraft returns from a direct Lunar-Earth trajectory. This paper analyzed the crew’s chest biomechanical response to assess potential injuries caused by acceleration loads during the re-entry phase. Initially, a sophisticated finite element model of the chest was constructed
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MicroRNA-155 mediates multiple gene regulations pertinent to the role of human adipose-derived mesenchymal stem cells in skin regeneration Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Hady Shahin, Luigi Belcastro, Jyotirmoy Das, Marina Perdiki Grigoriadi, Rolf B. Saager, Ingrid Steinvall, Folke Sjöberg, Pia Olofsson, Moustafa Elmasry, Ahmed T. El-Serafi
Introduction: The role of Adipose-derived mesenchymal stem cells (AD-MSCs) in skin wound healing remains to be fully characterized. This study aims to evaluate the regenerative potential of autologous AD-MSCs in a non-healing porcine wound model, in addition to elucidate key miRNA-mediated epigenetic regulations that underlie the regenerative potential of AD-MSCs in wounds.Methods: The regenerative
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Current developments and future perspectives of nanotechnology in orthopedic implants: an updated review Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Wenqing Liang, Chao Zhou, Juqin Bai, Hongwei Zhang, Hengguo Long, Bo Jiang, Haidong Dai, Jiangwei Wang, Hengjian Zhang, Jiayi Zhao
Orthopedic implants are the most commonly used fracture fixation devices for facilitating the growth and development of incipient bone and treating bone diseases and defects. However, most orthopedic implants suffer from various drawbacks and complications, including bacterial adhesion, poor cell proliferation, and limited resistance to corrosion. One of the major drawbacks of currently available orthopedic
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Morphology and transverse alignment of the patella have no effect on knee gait characteristics in healthy Chinese adults over the age of 40 years Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Zhengming Wang, Jiehang Lu, Haiya Ge, Zhengyan Li, Min Zhang, Fuwei Pan, Rui Wang, Hengkai Jin, Guangyue Yang, Zhibi Shen, Guoqing Du, Hongsheng Zhan
Background: The influence of patella morphology and horizontal alignment on knee joint kinematics and kinetics remains uncertain. This study aimed to assess patella morphology and transverse alignment in relation to knee kinetics and kinematics in individuals without knee conditions. A secondary objective was to investigate the impact of femur and tibia alignment and shape on knee gait within this
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How to make full use of dental pulp stem cells: an optimized cell culture method based on explant technology Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 You Wu, Jiangling Sun, Wang Wang, Yao Wang, Reinhard E. Friedrich
Introduction:Dental pulp stem cells from humans possess self-renewal and versatile differentiation abilities. These cells, known as DPSC, are promising for tissue engineering due to their outstanding biological characteristics and ease of access without significant donor site trauma. Existing methods for isolating DPSC mainly include enzyme digestion and explant techniques. Compared with the enzymatic
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Evaluation of nanoparticle albumin-bound paclitaxel loaded macrophages for glioblastoma treatment based on a microfluidic chip Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Zuorun Xie, Junyi Ye, Xinghua Gao, Hang Chen, Maosong Chen, Jiangfang Lian, Jingyun Ma, Hongcai Wang
Introduction: Glioblastoma (GBM) is a primary brain malignancy with a dismal prognosis and remains incurable at present. In this study, macrophages (MΦ) were developed to carry nanoparticle albumin-bound paclitaxel (nab-PTX) to form nab-PTX/MΦ. The aim of this study is to use a GBM-on-a-chip to evaluate the anti-GBM effects of nab-PTX/MΦ.Methods: In this study, we constructed nab-PTX/MΦ by incubating
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Optimization of number and range of shunt valve performance levels in infant hydrocephalus: a machine learning analysis Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Mark Graham Waterstraat, Arshia Dehghan, Seifollah Gholampour
Shunt surgery is the main treatment modality for hydrocephalus, the leading cause of brain surgery in children. The efficacy of shunt surgery, particularly in infant hydrocephalus, continues to present serious challenges in achieving improved outcomes. The crucial role of correct adjustments of valve performance levels in shunt outcomes has been underscored. However, there are discrepancies in the
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Genetically engineered eucalyptus expressing pesticidal proteins from Bacillus thuringiensis for insect resistance: a risk assessment evaluation perspective Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-18 Dror Avisar, Alexandre Manoeli, Anselmo Azevedo dos Santos, Antonio Carlos Da Mota Porto, Carolina Da Silva Rocha, Edival Zauza, Esteban R. Gonzalez, Everton Soliman, José Mateus Wisniewski Gonsalves, Lorena Bombonato, Maria P. Galan, Maurício M. Domingues, Murici Carlos Candelaria, Reginaldo Mafia, Rodrigo Neves Graça, Shelly Azulay, Sivan Livne, Tatiane Buono Dias, Thaís Regina Drezza, William Jose
Eucalyptus covers approximately 7.5 million hectares in Brazil and serves as the primary woody species cultivated for commercial purposes. However, native insects and invasive pests pose a significant threat to eucalyptus trees, resulting in substantial economic losses and reduced forest productivity. One of the primary lepidopteran pests affecting eucalyptus is Thyrinteina arnobia (Stoll, 1782) (Lepidoptera:
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Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-15 Aleksandra Bandzerewicz, Joanna Howis, Kamil Wierzchowski, Miroslav Slouf, Jiri Hodan, Piotr Denis, Tomasz Gołofit, Maciej Pilarek, Agnieszka Gadomska-Gajadhur
Biomaterials containing citric acid as a building unit show potential for use as blood vessel and skin tissue substitutes. The success in commercializing implants containing a polymer matrix of poly(1,8-octanediol citrate) provides a rationale for exploring polycitrates based on other diols. Changing the aliphatic chain length of the diol allows functional design strategies to control the implant’s
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Orbital shaking conditions augment human nasoseptal cartilage formation in 3D culture Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-15 Thomas Harry Jovic, Feihu Zhao, Henry Jia, Shareen Heather Doak, Iain Stuart Whitaker
Introduction: This study aimed to determine whether a dynamic orbital shaking culture system could enhance the cartilage production and viability of bioengineered nasoseptal cartilage.Methods: Human nasal chondrocytes were seeded onto nanocellulose-alginate biomaterials and cultured in static or dynamic conditions for 14 days. Quantitative polymerase chain reaction for chondrogenic gene expression
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Ethical implications of developing RNA-based therapies for cardiovascular disorders Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-15 Mihaela Hostiuc, Alexandru Scafa, Bogdan Iancu, Daniela Iancu, Oana-Maria Isailă, Oana Mihaela Ion, Ana Stroe, Camelia Diaconu, Dragos Epistatu, Sorin Hostiuc
The awareness concerning RNA-based therapies was boosted significantly after the successful development of COVID-19 vaccines. However, they can potentially lead to significant advances in other areas of medicine, such as oncology or chronic diseases. In recent years, there has been an exponential increase in the number of RNA-based therapies that were evaluated as potential treatments for cardiovascular
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Targeted drug delivery of engineered mesenchymal stem/stromal-cell-derived exosomes in cardiovascular disease: recent trends and future perspectives Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-15 Jian-Liang Pang, Hong Shao, Xiao-Gang Xu, Zhi-Wei Lin, Xiao-Yi Chen, Jin-Yang Chen, Xiao-Zhou Mou, Pei-Yang Hu
In recent years, stem cells and their secretomes, notably exosomes, have received considerable attention in biomedical applications. Exosomes are cellular secretomes used for intercellular communication. They perform the function of intercellular messengers by facilitating the transport of proteins, lipids, nucleic acids, and therapeutic substances. Their biocompatibility, minimal immunogenicity, targetability
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The influence of internal pressure and neuromuscular agents on C. elegans biomechanics: an empirical and multi-compartmental in silico modelling study Front. Bioeng. Biotech. (IF 5.7) Pub Date : 2024-03-15 Clara L. Essmann, Muna Elmi, Christoforos Rekatsinas, Nikolaos Chrysochoidis, Michael Shaw, Vijay Pawar, Mandayam A. Srinivasan, Vasileios Vavourakis
The function of a specific tissue and its biomechanics are interdependent, with pathologies or ageing often being intertwined with structural decline. The biomechanics of Caenorhabditis elegans, a model organism widely used in pharmacological and ageing research, has been established as biomarker for healthy ageing. However, the properties of the constituent tissues, and their contribution to the overall