样式: 排序: IF: - GO 导出 标记为已读
-
Phenylbutyric acid inhibits hypoxia‐induced trophoblast apoptosis and autophagy in preeclampsia via the PERK/ATF‐4/CHOP pathway Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-04-22 Yinfeng Li, Yongjie Guo, Dan Wu, Ling Ai, Rongrong Wu, Zepeng Ping, Kangyuan Zhu
Preeclampsia (PE) is a common pregnancy complication with a high mortality rate. Abnormally activated endoplasmic reticulum stress (ERS) is believed to be responsible for the destruction of key placental cells‐trophoblasts. Phenylbutyric acid (4‐PBA), an ERS inhibitor, is involved in regulating the development of ERS‐related diseases. At present, how 4‐PBA affects trophoblasts and its mechanisms is
-
-
CPEB1 induces autophagy and promotes apoptosis in ovarian granulosa cells of polycystic ovary syndrome Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-04-15 Feilan Xuan, Yuefang Ren, Jiali Lu, Weimei Zhou, Ruiying Jin, Aixue Chen, Yongju Ye
Inflammatory damage in ovarian granulosa cells (GCs) is a key mechanism in polycystic ovary syndrome (PCOS), cytoplasmic polyadenylation element binding protein‐1 (CPEB1) is important in inflammatory regulation, however, its role in PCOS is unclear. We aim to research the mechanism of CPEB1 in ovarian GCs in PCOS using dehydroepiandrosterone (DHEA)‐induced PCOS rat models and testosterone‐incubated
-
Fetal Leydig cells: What we know and what we don't Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-03-14 Keer Jiang, Joan S. Jorgensen
During male fetal development, testosterone plays an essential role in the differentiation and maturation of the male reproductive system. Deficient fetal testosterone production can result in variations of sex differentiation that may cause infertility and even increased tumor incidence later in life. Fetal Leydig cells in the fetal testis are the major androgen source in mammals. Although fetal and
-
Kojic acid inhibits pig sperm apoptosis and improves capacitated sperm state during liquid preservation at 17°C Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-03-11 Bingyan Hu, Haize Zhang, Yuqing Li, Qin Xue, Menghao Yang, Chaoyue Cao, Lei Gao, Guiyan Chu, Rui Cai, Yi Zheng, Weijun Pang
The parameters of sperm apoptosis and capacitation during liquid storage at 17°C can indicate the quality of pig sperm and the potential development of early embryos. However, the effect of kojic acid (KA) on semen preservation and its mechanism has not been fully understood. In this study, we discovered that adding KA to the diluent improved the antioxidant capacity of sperm mitochondria, maintained
-
-
Dynamics of extracellular vesicle uptake by mural granulosa cells in mice Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-03-07 Taichi Edure, Yuta Matsuno, Kodai Matsushita, Natsumi Maruyama, Wataru Fujii, Kunihiko Naito, Koji Sugiura
Extracellular vesicles (EVs) play an important role in the development and function of mammalian ovarian follicles. However, the mechanisms by which they are taken up by the follicular granulosa cells remain unclear. In addition, while oocytes play a pivotal role in follicular development, the possible interactions between oocyte‐derived paracrine factors (ODPFs) and EV signals are unknown. Therefore
-
Plasma‐derived from human umbilical cord blood restores ovarian function and improves serum reproductive hormones levels in mice with premature ovarian insufficiency (POI) through cytokines and growth factors Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-02-26 Tie Cheng Sun, Yi Ming Guo, Dong Mei Li, Yu Jie Wen, Zhu Xue Jiao, Ju Li, Hui Ping Wang, Yan Jun Jia, Shan Jie Zhou
Premature ovarian insufficiency (POI) patients experience a decline in ovarian function and a reduction in serum reproductive hormones, leading to a significant impact on the outcomes of assisted reproductive technology. Despite the absence of an effective clinical treatment to restore fertility in POI patients, recent research has indicated that cord blood plasma (CBP) derived from human umbilical
-
Fertilization, pregnancy, and neonatal outcomes after IVF, rescue ICSI, and ICSI in unexplained infertility: A retrospective study Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-02-02 Fenglong Qiu, Yanghua Zuo, Huiying Xue, Rongxue Zhang
Conventional intracytoplasmic sperm injection (ICSI) is not recommended for nonmale infertile patients to avoid fertilization failure (FF) in view of controversies regarding safety issues. Among the strategies that may help to promote the use of in vitro fertilization (IVF) for women with a high risk of FF, rescue ICSI (R-ICSI) has attracted attention. This study aimed to evaluate the efficacy of short-term
-
Proteomic analysis of boar seminal plasma: Putative markers for fertility based on capacity of semen preservation at 17°C Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-01-28 Matheus S. Lucca, Ivan C. Bustamante-Filho, Rafael R. Ulguim, Rafael D. F. Gianluppi, Joseph A. M. Evaristo, Fábio C. S. Nogueira, Luís F. S. M. Timmers, Ana P. G. Mellagi, Ivo Wentz, Fernando P. Bortolozzo
-
Cystic ovary disease (COD) alters structure and function of the bovine oviduct Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-01-25 Deirdre Scully, Sven Reese, Sabine Kölle
Cystic ovary disease (COD) is a common cause of subfertility in dairy cattle. Therefore, the aim of this study was to provide novel concepts for cyst classification and to investigate the effects of COD on tubal microarchitecture, oviductal metabolic function, and the formation of the sperm reservoir. Bovine Fallopian tubes affected by follicular cysts, follicular cysts with luteinization and luteal
-
hsd17b1 is a key gene for ovarian differentiation of the Siberian sturgeon Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-01-25 André Lasalle, Germán Benech-Correa, Frédéric G. Brunet, Denise Vizziano-Cantonnet
This is the first work using gonads from undifferentiated, genetically-sexed Siberian sturgeon describing expression changes in genes related to steroid synthesis and female and male sex differentiation. One factor identified as relevant for ovarian differentiation was the gene coding for the enzyme Hsd17b1, which converts estrone into estradiol-17β. hsd17b1 was highly activated in female gonads at
-
Dynamics of seminal fluid production after mating Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-01-25 Yvonne Kortsmit, Janine Mariën, Joris M. Koene, Yumi Nakadera
Seminal fluid proteins (SFPs) play vital roles for optimizing reproductive success in diverse animals. Underlining their significance, SFP production and transfer are highly plastic, e.g., depending on the presence of rivals or mating status of partners. However, surprisingly little is known about replenishing SFPs after mating. This is especially relevant in species that mate multiple times, as they
-
Deletion of the Sodium Glucose Cotransporter 1 (Sglt-1) impairs mouse sperm movement Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-01-23 September Numata, Mumtarin Jannat Oishee, Jeffrey McDermott, Hermann Koepsell, Volker Vallon, Gustavo Blanco
The Sodium Glucose Cotransporter Isoform 1 (Sglt-1) is a symporter that moves Na+ and glucose into the cell. While most studies have focused on the role of Sglt-1 in the small intestine and kidney, little is known about this transporter's expression and function in other tissues. We have previously shown that Sglt-1 is expressed in the mouse sperm flagellum and that its inhibition interferes with sperm
-
The targeting of DAPK1 with miR-190a-3p promotes autophagy in trophoblast cells Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-01-23 Qi-qi Luo, Yu Tian, Guang-jin Qu, Kun Huang, Pan-pan Hu, Ying Xue, Bi-feng Hu, Shan-shun Luo
Pre-eclampsia (PE) is a dangerous pathological status that occurs during pregnancy and is a leading reason for both maternal and fetal death. Autophagy is necessary for cellular survival in the face of environmental stress as well as cellular homeostasis and energy management. Aberrant microRNA (miRNA) expression is crucial in the pathophysiology of PE. Although studies have shown that miRNA (miR)-190a-3p
-
Long noncoding RNAs in human reproductive processes and diseases Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-01-18 Le Zhang, Hailong Sun, Xiujuan Chen
Infertility has become a global disease burden. Although assisted reproductive technologies are widely used, the assisted reproduction birth rate is no more than 30% worldwide. Therefore, understanding the mechanisms of reproduction can provide new strategies to improve live birth rates and clinical outcomes of enhanced implantation. Long noncoding RNAs (lncRNAs) have been reported to exert regulatory
-
Preimplantation genetic testing: A remarkable history of pioneering, technical challenges, innovations, and ethical considerations Mol. Reprod. Dev. (IF 2.5) Pub Date : 2024-01-18 Keith E. Latham
Preimplantation genetic testing (PGT) has emerged as a powerful companion to assisted reproduction technologies. The origins and history of PGT are reviewed here, along with descriptions of advances in molecular assays and sampling methods, their capabilities, and their applications in preventing genetic diseases and enhancing pregnancy outcomes. Additionally, the potential for increasing accuracy
-
Germ plasm dynamics during oogenesis and early embryonic development in Xenopus and zebrafish Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-12-21 Divyanshi, Jing Yang
Specification of the germline and its segregation from the soma mark one of the most crucial events in the lifetime of an organism. In different organisms, this specification can occur through either inheritance or inductive mechanisms. In species such as Xenopus and zebrafish, the specification of primordial germ cells relies on the inheritance of maternal germline determinants that are synthesized
-
Sperm, eggs, pollen, and gelato, oh my! Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-12-06 Emma Whittington, Murielle Ålund
On September 4, 2023, the sky cleared over Nynäshamn, Sweden, and researchers from across the globe gathered for the 16th Biology of Spermatozoa (BoS) meeting. What followed was a week fuelled by tasty food (find out below about the gelato!) and beautiful weather discussing sperm, eggs, reproductive fluids, fertility, and all things reproductive evolution. Held biennially from its inception in the
-
Conservation and contrast in cell states of echinoderm ovaries Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-12-06 Nathalie Oulhen, Shumpei Morita, Cosmo Pieplow, Thomas M. Onorato, Stephany Foster, Gary Wessel
Echinoderms produce functional gametes throughout their lifespan, in some cases exceeding 200 years. The histology and ultrastructure of echinoderm ovaries has been described but how these ovaries function and maintain the production of high-quality gametes remains a mystery. Here, we present the first single cell RNA sequencing data sets of mature ovaries from two sea urchin species (Strongylocentrotus
-
Significant alteration of protein profiles in a mouse model of polycystic ovary syndrome Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-12-06 Bin Meng, Xiaoning Yang, Shiwei Luo, Chong Shen, Jia Qi, Haifeng Zhang, Yandong Li, Ying Xue, Juan Zhao, Pengxiang Qu, Enqi Liu
Polycystic ovary syndrome (PCOS) is an endocrine disorder, affecting women of child-bearing age, and the incidence rate is growing and assuming epidemic proportions. The etiology of PCOS remains unknown and there is no cure. Some animal models for PCOS have been established which have enhanced our understanding of the underlying mechanisms, but omics data for revealing PCOS pathogenesis and for drug
-
Pyroptosis is involved in maternal nicotine exposure-induced metabolic associated fatty liver disease progression in offspring mice Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-11-28 Yu-Qing Su, Yan Lin, Shu-Jing Huang, Yan-Ting Lin, Jing Ran, Fang-Fang Yan, Xian-Lan Liu, Long-Cheng Hong, Mei Huang, Huan-Zhong Su, Xiao-Dong Zhang, Jian-Hong You, Yi-Ming Su
We have investigated whether inflammasomes and pyroptosis are activated in maternal nicotine exposure (MNE) offspring mice and whether they are involved in MNE-promoted metabolic associated fatty liver disease (MAFLD) in adult offspring. We injected pregnant mice subcutaneously with saline vehicle or nicotine twice a day on gestational days 11–21. Offspring mice from both groups were fed with a normal
-
Epigenetic aging of mammalian gametes Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-11-24 Michael Klutstein, Nitzan Gonen
The process of aging refers to physiological changes that occur to an organism as time progresses and involves changes to DNA, proteins, metabolism, cells, and organs. Like the rest of the cells in the body, gametes age, and it is well established that there is a decline in reproductive capabilities in females and males with aging. One of the major pathways known to be involved in aging is epigenetic
-
FAM71D is dispensable for spermatogenesis and male fertility in mice Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-11-22 Shaomei Mo, Keming Deng, Congcong Cao, Yaoting Gui, Qian Ma
In mammals, the generation of sperm cells capable of fertilization is a highly complex process including spermatogenesis in the testis and maturation in the epididymis. In our previous study, we have demonstrated that FAM71D (Family with sequence similarity 71, member D), which could interact with calmodulin, was highly expressed in human and mouse testis. To investigate the physiological role of FAM71D
-
The molecular mechanism of naringin improving endometrial receptivity of OHSS rats Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-11-14 Lan Yuan, Yulin Li, Xueping Li, Zhu Mao, Yi Liu, Chengzhi Feng, Rongxing Jiang
Controlling ovarian hyperstimulation syndrome (OHSS) in the controlled ovarian hyperstimulation treatment is necessary to increase the implantation success rate. This study aimed to explore the effect of naringin on the endometrial receptivity of OHSS rats. Female rats were randomly assigned to six groups: Blank, model, low-dose naringin (100 mg/kg/day), medium-dose naringin (200 mg/kg/day), high-dose
-
Long-term storage does not affect the DNA methylation profiles of vitrified-warmed human embryos Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-10-26 Ling Zhu, Liwei Sun, Weiwei Liu, Wei Han, Guoning Huang, Jingyu Li
With the widespread application of embryo cryopreservation in assisted reproductive techniques, it is necessary to assess the safety of long-term cryopreservation of human embryos and it is unclear whether storage time has an impact on the DNA methylation profiles of human embryos. Nine women who received IVF treatment were recruited for this study. The retrieved eight-cell human embryos were classified
-
Electroporation-mediated genome editing in vitrified/warmed porcine zygotes obtained in vitro Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-10-26 Seiki Haraguchi, Thanh Q. Dang-Nguyen, Kazuhiro Kikuchi, Tamás Somfai
Clustered regularly interspaced short palindromic repeats (CRISPR)-associated 9 (Cas9) system is the most efficient and widely used technology for genome editing in all sorts of organisms, including livestock animals. Here, we examined the feasibility of CRISPR/Cas9-derived genome editing (GE) in vitrified porcine zygotes, where the flexible planning of experiments in time and space is expected. OCT4
-
Long-term culture induces Bax-dependent apoptosis in rat preimplantation embryos Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-10-13 Kazuomi Nakamura, Misako Seno, Yuki Yoshimura, Osamu Suzuki
Although rat preimplantation embryos are necessary for producing genetically modified rats, their in vitro culture remains a challenge. Rat zygotes can develop from the one-cell stage to the blastocyst stage in vitro; however, long-term culture reduces their developmental competence via an unknown mechanism. In this study, we examined how in vitro conditions affect rat preimplantation embryos, which
-
A strong foundation for the next generation Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-10-09 Bettina P. Mihalas, Emily R. Frost, Kaushiki M. Kadam, Irene E. Sucquart, Prakruti Sirigeri, William L. Ledger, Robert B. Gilchrist
CONFLICT OF INTEREST STATEMENT The authors declare no conflict of interest.
-
A pilot study on the relationship between thrombospondin-1 (THBS1) and transforming growth factor beta1 (TGFβ1) in the bovine placenta during early mid-pregnancy as well as parturition with normally released and retained placenta Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-10-09 Jacek Wawrzykowski, Monika Jamioł, Marta Kankofer
-
The cell volume-regulatory glycine transporter GLYT1 is activated following metallopeptidase-mediated detachment of the oocyte from the zona pellucida Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-10-09 Chyna S. Ortman, Jay M. Baltz
Independent cell volume regulation is first acquired by the oocyte in two steps that occur during meiotic maturation: (1) activation of the glycine transporter GLYT1 (Slc6a9) that mediates the intracellular accumulation of glycine to provide osmotic support in the mature egg and early preimplantation embryo, and (2) release of the oocyte from the strong attachment to its rigid extracellular matrix
-
HDAC4 regulates the proliferation, migration, and invasion of trophoblasts in pre-eclampsia through the miR-134-5p/FOXM1 axis Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-09-28 Yanli Xu, Xiaodi Kang, Hongli Jiang, Huafang Liu, Wenjing Wang
Epigenetics, including histone modifications and noncoding RNAs, affects abnormal placental function in pre-eclampsia (PE). This study was conducted to explore the role of histone deacetylase 4 (HDAC4) in trophoblast invasion and migration. The expression levels of HDAC4, microRNA (miR)-134-5p, and forkhead box protein M1 (FOXM1) in placentas from PE patients and healthy controls and their correlations
-
Mevalonate pathway and male reproductive aging Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-09-21 Jia-Le Zhang, Meng Lv, Chao-Fan Yang, Ying-Xi Zhu, Chao-Jun Li
-
Trimethylation profile of histones H3 lysine 4 and 9 in late preantral and early antral caprine follicles grown in vivo versus in vitro in the presence of anethole Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-09-06 Ana F. B. Silva, Ana N. P. Morais, Laritza F. Lima, Anna C. A. Ferreira, Renato F. Silva, Naiza A. R. Sá, Satish Kumar, Ariclécio C. Oliveira, Benner G. Alves, Ana P. R. Rodrigues, Eduardo L. Gastal, Vilceu Bordignon, José R. Figueiredo
-
Control of pig reproduction XI Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-08-31 Clay A. Lents, Nicoline M. Soede
11th International Conference on Pig Reproduction 5th – 7th June 2023 Ghent Belgium
-
Metabolomic evolution of the postpartum dairy cow uterus Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-08-26 Nicolas Aranciaga, Alastair B. Ross, James D. Morton, Robin McDonald, Jessica L. Gathercole, Debra K. Berg
-
Participation of apoptotic markers in the process of maturation and elimination of spermatozoa in the epididymis of the Corynorhinus mexicanus bat Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-08-11 Gihovani Ademir Samano-Barbosa, Blanca Patrícia López-Trinidad, Ahiezer Rodríguez-Tobón, Julio Cesar Chávez-Zamora, Edith Cortés-Barberena, Miguel Angel León-Galván, Ernesto Rodríguez-Tobón, Edith Arenas-Ríos
The Corynorhinus mexicanus bat is characterized by a specific form of reproductive asynchrony between males and females. After mating, some sperm remain in the male's epididymis, the organ where the sperm had matured. It has not yet been determined if apoptotic markers participate in the process of the maturation and/or elimination of these cells, so studying this topic is essential for our understanding
-
lncRNA GHET1 regulates extravillous trophoblastic phenotype via EZH2/LSD1-mediated MT2A epigenetic suppression in pre-eclampsia Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-08-07 Pengyun Wan, Jia Huang, Wenting Liu, Xiaoyan Su, Bei Zhao, Xianggang Wang, Lu Zhao
Pre-eclampsia (PE) is usually defined as new-onset hypertension with albuminuria or other organ damage. Herein, the role and mechanism of long noncoding RNA (lncRNA) gastric carcinoma high expressed transcript 1 (GHET1) during PE are investigated. Expression of GHET1 in PE pregnancies was evaluated using quantitative real-time polymerase chain reaction (qRT-PCR). Proliferation and cell cycle of extravillous
-
Inhibition of phosphodiesterase PDE8B reduces activation of primordial follicles in mouse ovaries Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-07-27 Julie Feld Madsen, Mahboobeh Amoushahi, Christian Posselt Choi, Stine Bundgaard, Anders Heuck, Karin Lykke-Hartmann
In the ovaries, cyclic adenosine 3′,5′-monophosphate (cAMP) is a second messenger supporting the generation of steroids. Phosphodiesterases (PDEs) are regulators of intracellular cAMP, and therefore, potential regulators of ovarian function. Interestingly, the family of PDE genes are differentially expressed in human oocytes and granulosa cells from primordial and primary follicles, suggesting diverse
-
The expression levels of NOS2, HMOX1, and VEGFC in cumulus cells are markers of oocyte maturation and fertilization rate Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-07-24 Montserrat Barragán, David Cornet-Bartolomé, Natalia Molina, Rita Vassena
Throughout the reproductive life of women, cumulus cells (CC) protect the dormant oocyte from damage, act as sensors of the follicular microenvironment, and act as a gatekeeper for oocyte developmental potential. One such mechanism relies on the hypoxia-tolerance response, which, with age, decreases systematically, including in the ovary. We aimed to evaluate the association between gene expression
-
Hsa_circ_0001740 mediates trophoblast cell function via regulating miR-188-3p/ARRDC3 Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-07-12 Mei Long, Shan Wang
Preeclampsia is an obstetric disorder and remains the leading contributor to maternal and fetal morbidity and mortality. This study was designed to explore the role of hsa_circ_0001740 in preeclampsia as well as its underlying mechanism. Real-time quantitative polymerase chain reaction was performed to examine hsa_circ_0001740 and miR-188-3p levels in trophoblast cell line HTR-8/SVneo. The proliferation
-
Decreased LONP1 expression contributes to DNA damage and meiotic defects in oocytes Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-07-01 Chuanming Liu, Manlin Xu, Yajie Guan, Lilin Li, Wenwen Liu, Bichun Guo, Xiaoqiang Sheng, Yang Zhang, Jidong Zhou, Xin Zhen, Guijun Yan, Haixiang Sun, Lijun Ding
Meiotic defects in oocytes are the primary reason for decreased female fertility with advanced maternal age. In this study, we revealed that decreased expression of ATP-dependent Lon peptidase 1 (LONP1) in aged oocytes and oocyte-specific depletion of LONP1 disrupt oocyte meiotic progression accompanying with mitochondrial dysfunction. In addition, LONP1 downregulation increased oocyte DNA damage.
-
The constitutively active pSMAD2/3 relatively improves the proliferation of chicken primordial germ cells Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-06-28 Masumeh Zare, Seyed Ziaeddin Mirhoseini, Shahrokh Ghovvati, Saeed Yakhkeshi, Mahdi Hesaraki, Mojgan Barati, Forough Azam Sayyahpour, Hossein Baharvand, Seyedeh-Nafiseh Hassani
In many multicellular organisms, mature gametes originate from primordial germ cells (PGCs). Improvements in the culture of PGCs are important not only for developmental biology research, but also for preserving endangered species, and for genome editing and transgenic animal technologies. SMAD2/3 appear to be powerful regulators of gene expression; however, their potential positive impact on the regulation
-
Time-lapse monitoring: An adjunct tool to select embryos for preimplantation genetic testing Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-06-19 Daniela P. de Almeida Ferreira Braga, Amanda S. Setti, Patricia Guilherme, Christina Morishima, Assumpto Iaconelli, Edson Borges
The goal for the present study was to investigate the effect of aneuploidy on embryo morphokinetics events in a time-lapse imaging (TLI) system incubator. This retrospective cohort study was performed in a private university-affiliated in vitro fertilization center, between 2019 March and December 2020. Kinetic data were analyzed in 935 embryos, derived from 316 patients undergoing intracytoplasmic
-
Sperm capacitation as a predictor of boar fertility Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-06-12 Alexandra Keller, Karl Kerns
Prediction of a boar's fertility level has great economic importance for sow herds. After standard sperm morphology and motility metrics are met, approximately 25% of boars have less than 80% conception rates. Due in part to the many factors involved in the fertilization process, a multifactorial model incorporating multiple relevant sperm physiology factors will likely lead to increased understanding
-
Mitochondrial energy metabolism is downregulated in repeated implantation failure patients related to alteration of PGC-1α acetylation level Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-05-27 Shuo Han, Sai Liu, Nai-Qian Jin, Ya-Su Lv, Mo Yang, Shuai Ma, Yao Fu, Shan-Ke Zhao, Ming-Hui Liu
Herein we aimed at exploring mitochondrial energy metabolism status in patients with repeated implantation failure (RIF) and whether key regulatory factor PGC-1α of energy metabolism is involved in the decidualization of endometrial stromal cells. Mitochondrial oxidative phosphorylation level and ATP synthesis were compared in primary endometrial stromal cells from RIF and control group. At the same
-
Selecting the optimal strategies when using nurse sows for supernumerous piglets Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-05-21 Thomas Sønderby Bruun, Trine Friis Pedersen, Flemming Thorup, Anja Varmløse Strathe
Hyper-prolific sows frequently do not have a sufficient number of functional teats for their piglets to nurse which has led to the use of nurse sows to manage these surplus piglets. This review discusses strategies for using nurse sows and factors that influence preweaning survival and weight gain of their litters, as well as those that affect their subsequent rebreeding performance. Rearing piglets
-
Histochemical and immunohistochemical analysis of testicular morphology and spermatogenesis in Gymnotus carapo (Teleostei: Gymnotidae) Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-05-06 Méndez Galarza Sabrina, Olea Gabriela, Blanco Cohene Tania, Perez Dante, Flores Quintana Carolina
-
RNA sequencing and expression analysis reveal a role for Lhx9 in the haploinsufficient adult mouse ovary Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-04-21 Stephanie Workman, Megan J. Wilson
Understanding the molecular pathways that underpin ovarian development and function is vital for improving the research approaches to investigating fertility. Despite a significant improvement in our knowledge of molecular activity in the ovary, many questions remain unanswered in the quest to understand factors influencing fertility and ovarian pathologies such as cancer. Here, we present an investigation
-
Functional annotation of a hugely expanded nanos repertoire in Lytechinus variegatus, the green sea urchin Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-04-11 Cosmo Pieplow, Gary Wessel
Nanos genes encode essential RNA-binding proteins involved in germline determination and germline stem cell maintenance. When examining diverse classes of echinoderms, typically three, sometimes four, nanos genes are present. In this analysis, we identify and annotate nine nanos orthologs in the green sea urchin, Lytechinus variegatus (Lv). All nine genes are transcribed and grouped into three distinct
-
Cumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-04-11 Thainara C. F. Silva, Margot A. N. Dode, Thiago F. Braga, Mariana G. Marques, Luna Nascimento Vargas, Otávio Augusto Costa de Faria, Andressa P. de Souza, Daniela Albring, Alexandre R. Caetano, Maurício M. Franco
This study aimed to evaluate the effects of donor age on lipid metabolism during in vitro maturation (IVM) of pigs cumulus-oocyte complexes (COCs). We evaluated transcript levels of genes, the percentage of ooplasm occupied by lipid droplets (LD) and evaluated DNA methylation in COCs from sows and prepubertal gilts. Transcript levels of six genes (ACACA, ACSS2, FASN, FABP3, SLC27A4, PLIN2), which were
-
The vascular endothelial growth factor (VEGF) system as a key regulator of ovarian follicle angiogenesis and growth Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-03-26 Adrian Guzmán, Cyndi G. Hernández-Coronado, Carlos G. Gutiérrez, Ana M. Rosales-Torres
The vascular endothelial growth factor-A (VEGFA) system is a complex set of proteins, with multiple isoforms and receptors, including both angiogenic (VEGFxxx, VEGFR2) and antiangiogenic members (VEGFxxxb, VEGFR1 and soluble forms of VEGFR). The members of the VEGF system affect the proliferation, survival, and migration of endothelial and nonendothelial cells and are involved in the regulation of
-
Jun-mediated lncRNA-IMS promotes the meiosis of chicken spermatogonial stem cells via gga-miR-31-5p/stra8 Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-03-26 Yingjie Wang, Lei Zhang, Ruihong Kong, Cai Hu, Zongyi Zhao, Yuhui Wu, Qisheng Zuo, Bichun Li, Ya-ni Zhang
Meiosis, a key step in spermatogenesis, is affected by many factors. Current studies have shown that long noncoding RNAs (lncRNAs) are potential factors regulating meiosis, and their regulatory mechanisms have received much attention. However, little research has been done on its regulatory mechanism in the spermatogenesis of roosters. Here, we found that lncRNA involved in meiosis and spermatogenesis (lncRNA-IMS)
-
Rapamycin encourages the maintenance of mitochondrial dynamic balance and mitophagy activity for improving developmental competence of blastocysts in porcine embryos in vitro Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-03-21 Hyo-Jin Park, Gyeong-Deok Heo, Seul-Gi Yang, Deog-Bon Koo
Rapamycin induces autophagosome formation and activity during oocyte maturation, improved fertilization ability of matured oocytes, and early embryonic developmental competence. However, potential changes in mitochondrial fission and mitophagy via regulation of autophagy in early porcine embryonic development have not been previously studied. Here, we investigated embryonic developmental ability and
-
High level of thyroid peroxidase antibodies as a detrimental risk of pregnancy outcomes in euthyroid women undergoing ART: A meta-analysis Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-03-15 Sudan Zhang, Mingdong Yang, Teng Li, Min Yang, Wei Wang, Yunqing Chen, Yu Ding, Jianxin Liu, Xiaohui Xu, Jian Zhang, Zheng Wang, Jiane Liu
Thyroid autoimmunity (TAI) triggered by genetic and epigenetic variation occurs mostly in women of reproductive age. TAI is described mainly by positivity of anti-thyroid peroxidase antibody (TPO-Ab) and/or thyroglobulin antibody (TG-Ab). TPO-Ab, but not TG-Ab, was suggested to be associated with pregnancy outcome in euthyroid women undergoing assisted reproductive technology (ART), but their results
-
RACK1 may participate in placental development at mid-gestation via regulating trophoblast cell proliferation and migration in pigs Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-03-13 Zhimin Wu, Guangling Hu, Ting Gong, Qun Hu, Linjun Hong, Yiyu Zhang, Zheng Ao
Intrauterine growth restriction (IUGR) is a severe complication in swine production. Placental insufficiency is responsible for inadequate fetal growth, but the specific etiology of placental dysfunction-induced IUGR in pigs remains poorly understood. In this work, placenta samples supplying the lightest weight (LW) and mean weight (MW) pig fetuses in the litter at Day 65 (D65) of gestation were collected
-
Role of intraluteal and intrauterine prostaglandin signaling in LH-induced luteolysis in pregnant rats Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-03-02 Akshi Vashistha, Habibur Rahaman Khan
Luteal dysfunctions lead to fertility disorders and pregnancy complications. Normal luteal function is regulated by many factors, including luteinizing hormone (LH). The luteotropic roles of LH have been widely investigated but its role in the process of luteolysis has received little attention. LH has been shown to have luteolytic effects during pregnancy in rats and the role of intraluteal prostaglandins
-
Reconsidering “low-dose”—Impacts of oral estrogen exposure during preimplantation embryo development Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-03-02 Meret Schmidhauser, Anna-Katharina Hankele, Susanne E. Ulbrich
Perturbations of estrogen signaling during developmental stages of high plasticity may lead to adverse effects later in life. Endocrine-disrupting chemicals (EDC) are compounds that interfere with the endocrine system by particularly mimicking the action of endogenous estrogens as functional agonists or antagonists. EDCs compose synthetic and naturally occurring compounds discharged into the environment
-
DNA methylation profile of single in vitro matured bovine oocytes Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-02-28 Luna N. Vargas, Felippe M. C. Caixeta, Margot A. N. Dode, Alexandre R. Caetano, Maurício M. Franco
-
Preimplantation embryo gene expression: 56 years of discovery, and counting Mol. Reprod. Dev. (IF 2.5) Pub Date : 2023-02-22 Keith E. Latham