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An optimized protocol for routine development of cell culture from adult oyster, Crassostrea madrasensis Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-27 Soumya Balakrishnan, Ambadi Kannan Maliyekkal Sajeevan, Sreevidya Chandrasekharan Parvathi, I. S. Bright Singh, Jayesh Puthumana
Marine molluscan cell lines, required for virus screening and cultivation, form essential tools for developing health management strategies for these animals in the blue economy. Moreover, they are also crucial to develop cultivated seafood. As there is no valid marine molluscan cell line, primary cell cultures are relied upon for all investigations. A sound protocol for generating primary cell cultures
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Translocator protein expression and localization in human endometrium and endometriosis: A potential target for a noninvasive diagnosis? Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-21 Maíra Casalechi, Cynthia Dela Cruz, Wiviane A. Assis, Millene Vieira‐Lopes, Felipe Eduardo F. Lopes, Antônio M.C. Francisco, Fernando M. Reis
The limitations of current imaging methods to detect small or superficial endometriotic lesions prompt the search for new molecular targets. TSPO is an 18 KDa protein located in the outer mitochondrial membrane, which can be traced by positron emission tomography (PET) using specific ligands. TSPO is located mostly in neurons and inflammatory sites outside the brain. We hypothesized that it might also
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Fibroblast activation protein promotes progression of hepatocellular carcinoma via regulating the immunity Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-19 Xiangcheng Wang, Ruilong Niu, Hao Yang, Yu Lin, Hui Hou, Hong Yang
Fibroblast activation protein (FAP) has been indicated to express in cancer‐associated fibroblasts (CAFs) in most cancers. This work was dedicated to exploring FAP's effects on hepatocellular carcinoma (HCC). The data were extracted from The Cancer Genome Atlas, Gene Expression Omnibus, ImmPort, and Reactome databases. The correlation between FAP and HCC patients' prognosis was explored via survival
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Durotaxis: A cause of organ fibrosis and metastatic cancer? Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-19 Wolfgang H. Goldmann
Directional migration, in which cells move up matrix stiffness gradients independent of soluble factors (chemotaxis) or matrix-bound ligands (haptotaxis), is termed durotaxis (Lo et al., 2000). Advances in the development of bioengineered matrices with stiffness gradients have facilitated the study of cell durotaxis in vitro (Sunyer et al., 2016) and demonstrated the durotactic capacity of stem (Tse
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Silver nanoparticles stimulate 5‐Fluorouracil‐induced colorectal cancer cells to kill through the upregulation TRPV1‐mediated calcium signaling pathways Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-19 Müge Mavioğlu Kaya
The involvement of the TRP vanilloid 1 (TRPV1) cation channel on the 5‐Fluorouracil (5‐FU)‐caused Ca2+ signals through the activation of the apoptotic signaling pathway and stimulating the mitochondrial Ca2+ and Zn2+ accumulation‐induced reactive oxygen species (ROS) productions in several cancer cells, except the colorectal cancer (HT‐29) cell line, was recently reported. I aimed to investigate the
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Degradation meets development: Implications in β‐cell development and diabetes Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-19 Akshaya Ashok, Guruprasad Kalthur, Anujith Kumar
Pancreatic development is orchestrated by timely synthesis and degradation of stage‐specific transcription factors (TFs). The transition from one stage to another stage is dependent on the precise expression of the developmentally relevant TFs. Persistent expression of particular TF would impede the exit from the progenitor stage to the matured cell type. Intracellular protein degradation‐mediated
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Paynantheine more effectively reverses multidrug resistance in malignant EPG85.257RDB and MCF7MX cells than morphine and speciociliatine Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-14 Nayereh Abdali, Mohammad Nadipour Borujeni, Sayedeh Azimeh Hosseini, Rahim Malekzadeh, Marzieh Abolhasani, Seyed Abbas Mirzaei, Fatemeh Elahian
The possible interactions of morphine, paynantheine and speciociliatine alkaloids with ATP‐binding cassette (ABC) transporters was investigated. The compounds were docked against ABCG2 and ABCB1 to predict the binding mode of alkaloids in active binding sites. The cytotoxicity of morphine, paynantheine and speciociliatine for EPG85.257RDB and MCF7MX cells was determined and ABCB1 and ABCG2 gene and
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Curcumin relieves oxaliplatin‐induced neuropathic pain via reducing inflammation and activating antioxidant response Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-14 Meng‐Wei Zhang, Xu Sun, Yi‐Wen Xu, Wei Meng, Qiong Tang, Hui Gao, Ling Liu, Shao‐Hui Chen
Oxaliplatin (OXA) has shown high effectiveness in the treatment of cancers, but its anticancer clinical effects often induce neurotoxicity leading to neuropathic pain. Oxidative damage and NLRP3 inflammasome play important roles in neuropathic pain development. Here, neuropathic pain mouse model was constructed by continuous intraperitoneal injection of OXA. OXA administration induced mechanical pain
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Correction to “Autophagy regulates the apoptosis of bone marrow‐derived mesenchymal stem cells under hypoxic condition via AMP‐activated protein kinase/mammalian target of rapamycin pathway” Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-12
Zhang, Z., Yang, M., Wang, Y., Wang, L., Jin, Z., Ding, L., Zhang, L., Zhang, L., Jiang, W., Gao, G., Yang, J., Lu, B., Cao, F., & Hu, T. (2016) Autophagy regulates the apoptosis of bone marrow-derived mesenchymal stem cells under hypoxic condition via AMP-activated protein kinase/mammalian target of rapamycin pathway. Cell Biology Internationa, 40(6), 671–685. https://doi.org/10.1002/cbin.10604. In
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OxLDL enhances procoagulant activity of endothelial cells by TMEM16F‐mediated phosphatidylserine exposure Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-06 Meishan Yan, Zelong Wang, Yao An, Zhanni Li, Yun Li, Hongyu Zhang, Caixia Li, Lifeng Wang, Li Chen, Chao Gao, Dongsheng Wang, Chunyan Gao
Oxidized low‐density lipoprotein (oxLDL), a key component in atherosclerosis and hyperlipidemia, is a risk factor for atherothrombosis in dyslipidemia, yet its mechanism is poorly understood. In this study, we used oxLDL‐induced human aortic endothelial cells (HAECs) and high‐fat diet (HFD)‐fed mice as a hyperlipidemia model. Phosphatidylserine (PS) exposure, cytosolic Ca2+, reactive oxygen species
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Identification and validation of m6A‐associated ferroptosis genes in renal clear cell carcinoma Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-05 Shuo Pang, Shuo Zhao, Yuxi Dongye, Yidong Fan, Jikai Liu
Urinary cancer is synonymous with clear cell renal cell carcinoma (ccRCC). Unfortunately, existing treatments for this illness are ineffective and unpromising. Finding novel ccRCC biomarkers is crucial to creating successful treatments.The Cancer Genome Atlas provided clear cell renal cell carcinoma transcriptome data. Functional enrichment analysis was performed on ccRCC and control samples' differentially
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Dihydroartemisinin eliminates senescent cells by promoting autophagy‐dependent ferroptosis via AMPK/mTOR signaling pathway Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-05 Xing Wan, Can Li, Yue Hao Tan, Shi Qi Zuo, Feng Mei Deng, Jing Sun, Yi Lun Liu
Cellular senescence is an irreversible cell‐cycle arrest in response to a variety of cellular stresses, which contribute to the pathogenesis of a variety of age‐related degenerative diseases. However, effective antisenescence strategies are still lacking. Drugs that selectively target senescent cells represent an intriguing therapeutic strategy to delay aging and age‐related diseases. Thus, we thought
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Protective autophagy enhances antistress ability through AMPK/ULK1 signaling pathway in human immortalized keratinocytes Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-04 Zhinan Shi, Jing Wang, Min Li, Li Gu, Zhiyi Xu, Xiaoyu Zhai, Shu Zhou, Jingting Zhao, Liqun Gu, Lin Chen, Linling Ju, Bingrong Zhou, Hui Hua
Keratinocytes, located in the outermost layer of human skin, are pivotal cells to resist environmental damage. Cellular autophagy plays a critical role in eliminating damaged organelles and maintaining skin cell homeostasis. Low‐dose 5‐Aminolevulinic acid photodynamic therapy (ALA‐PDT) has been demonstrated to enhance skin's antistress ability; however, the regulatory mechanisms of autophagy in keratinocytes
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Follistatin‐like 1 protects against doxorubicin‐induced cardiotoxicity by preventing mitochondrial dysfunction through the SIRT6/Nrf2 signaling pathway Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-04 Haijun Xu, Hong Guo, Zhigang Tang, Ruijun Hao, Shaowei Wang, Ping Jin
Mitochondrial dysfunction and myocardial remodeling have been reported to be the main underlying molecular mechanisms of doxorubicin‐induced cardiotoxicity. SIRT6 is a nicotinamide adenine dinucleotide‐dependent enzyme that plays a vital role in cardiac protection against various stresses. Moreover, previous studies have demonstrated that FSTL1 could alleviate doxorubicin‐induced cardiotoxicity by
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CoCl2‐Induced hypoxia promotes hPDLSCs osteogenic differentiation through AKT/mTOR/4EBP‐1/HIF‐1α signaling and facilitates the repair of alveolar bone defects Cell Biol. Int. (IF 3.9) Pub Date : 2024-03-04 Zhibo Fan, Yanru Liu, Yuxin Lan, Yujie Wu, Junyu Li, Xiaomei Xu
Bone defects are characterized by a hypoxic environment, which affects bone tissue repair. However, the role of hypoxia in the repair of alveolar bone defects remains unclear. Human periodontal ligament stem cells (hPDLSCs) are high‐quality seed cells for repairing alveolar bone defects, whose behavior changes under hypoxia. However, their mechanism of action is not known and needs to be elucidated
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Epigenetic changes in shear‐stressed endothelial cells Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-29 Thaís Silva Pinto, Geórgia da Silva. Feltran, Célio Júnior da C. Fernandes, Amanda Fantini de Camargo Andrade, Alex de Camargo Coque, Simone L. Silva, Abdulwahab A. Abuderman, Willian F. Zambuzzi, Rodrigo A. Foganholi da Silva
Epigenetic changes, particularly histone compaction modifications, have emerged as critical regulators in the epigenetic pathway driving endothelial cell phenotype under constant exposure to laminar forces induced by blood flow. However, the underlying epigenetic mechanisms governing endothelial cell behavior in this context remain poorly understood. To address this knowledge gap, we conducted in vitro
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The ultrastructure of the apical organ of Curini‐Galletti's larva, a new polyclad larval type Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-29 Isabel L. Dittmann, Philip Bertemes, Clemens Gotsis, Alexandra L. Grosbusch, Stefan Redl, Michael W. Hess, Willi Salvenmoser, Bernhard Egger
Polycladida are the only free‐living flatworms with a planktonic larval stage in some species. Currently, it is not clear if a larval stage is ancestral in polyclads, and which type of larva that would be. Known polyclad larvae are Müller's larva, Kato's larva and Goette's larva, differing by body shape and the number of lobes and eyes. A valuable character for the comparison and characterisation of
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Assessment of human embryonic stem cells differentiation into definitive endoderm lineage on the soft substrates Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-29 Jasmeet Kaur Virdi, Prasad Pethe
Pluripotent stem cells (PSCs) hold enormous potential for treating multiple diseases owing to their ability to self‐renew and differentiate into any cell type. Albeit possessing such promising potential, controlling their differentiation into a desired cell type continues to be a challenge. Recent studies suggest that PSCs respond to different substrate stiffness and, therefore, can differentiate towards
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Noncoding RNAs and programmed cell death in hepatocellular carcinoma: Significant role of epigenetic modifications in prognosis, chemoresistance, and tumor recurrence rate Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-27 Reza Arefnezhad, Sara Ashna, Fatemeh Rezaei‐Tazangi, Seyed Mahdi Arfazadeh, Seyede Shabnam Seyedsalehie, Shaghayegh Yeganeafrouz, Melika Aghaei, Mandana Sanandaji, Ramin Davoodi, Sareh Ranjbar Karim Abadi, Massoud Vosough
Hepatocellular carcinoma (HCC) is the most common type of liver cancer with a high death rate in the world. The molecular mechanisms related to the pathogenesis of HCC have not been precisely defined so far. Hence, this review aimed to address the potential cross‐talk between noncoding RNAs (ncRNAs) and programmed cell death in HCC. All related papers in the English language up to June 2023 were collected
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Regulation of iron metabolism in HEC‐1A endometrium cells by macrophage‐derived factors and fractalkine Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-27 Edina Pandur, Ramóna Pap, Gergely Jánosa, Kitti Tamási, Katalin Sipos
Macrophages in the endometrium promote receptivity and implantation by secreting proinflammatory cytokines and other factors like fractalkine (FKN). Macrophages are closely linked to regulating iron homeostasis and can modulate iron availability in the tissue microenvironment. It has been revealed that the iron metabolism of the mother is crucial in fertility. Iron metabolism is strictly controlled
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CACNA1B protects naked mole‐rat hippocampal neuron from apoptosis via altering the subcellular localization of Nrf2 after 60Co irradiation Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-23 Wenjing Yang, Chao Chen, Xiaolong Jiang, Yining Zhao, Junyang Wang, Qianqian Zhang, Jingyuan Zhang, Yan Feng, Shufang Cui
Although radiotherapy is the most effective treatment modality for brain tumors, it always injures the central nervous system, leading to potential sequelae such as cognitive dysfunction. Radiation induces molecular, cellular, and functional changes in neuronal and glial cells. The hippocampus plays a critical role in learning and memory; therefore, concerns about radiation‐induced injury are widespread
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Histological study of telocytes in mice intrauterine adhesion model and their positive effect on mesenchymal stem cells in vitro Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-14 Xiaojiao Wei, Hui Li, Tianquan Chen, Xiaojun Yang
Intrauterine adhesions (IUA), the main cause of secondary infertility in women, result from irreversible fibrotic repair of the endometrium due to inflammation or human factors, accompanied by disruptions in the repair function of endometrial stem cells. This significantly impacts the physical and mental health of women in their childbearing years. Telocytes (TCs), a distinctive type of interstitial
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The noncanonical nucleotide binding site 1 of the bile salt export pump is optimized for proper function of the transporter Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-08 Imran Sohail, Mahmood Ul Hassan, Diethart Schmid, Peter Chiba
The bile salt export pump (ABCB11/BSEP) is a hepatocyte plasma membrane-resident protein translocating bile salts into bile canaliculi. The sequence alignment of the four full-length transporters of the ABCB subfamily (ABCB1, ABCB4, ABCB5 and ABCB11) indicates that the NBD-NBD contact interface of ABCB11 differs from that of other members in only four residues. Notably, these are all located in the
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3D bio scaffold support osteogenic differentiation of mesenchymal stem cells Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-06 Faiza Ramzan, Shumaila Khalid, Sobia Ekram, Asmat Salim, Trivia Frazier, Sumreen Begum, Omair A. Mohiuddin, Irfan Khan
The regeneration of osteochondral lesions by tissue engineering techniques is challenging due to the lack of physicochemical characteristics and dual-lineage (osteogenesis and chondrogenesis). A scaffold with better mechanical properties and dual lineage capability is required for the regeneration of osteochondral defects. In this study, a hydrogel prepared from decellularized human umbilical cord
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A novel PDIA3/FTO/USP20 positive feedback regulatory loop induces osteogenic differentiation of preosteoblast in osteoporosis Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-06 Fei Zhang, Chen Liu, Zhiyong Chen, Chengyi Zhao
Osteoporosis is a chronic skeletal disease and the major source of risk for fractures in aged people. It is urgent to investigate the mechanism regulating osteoporosis for developing potential treatment and prevention strategies. Osteogenic differentiation of preosteoblast enhances bone formation, which might be a promising strategy for treatment and prevention of osteoporosis. Protein disulfide isomerase
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M6A methylation-regulated autophagy may be a new therapeutic target for intervertebral disc degeneration Cell Biol. Int. (IF 3.9) Pub Date : 2024-02-05 Yuao Tao, Xiaoyu Yu, Xiaolong Li, Yuzhu Xu, Hui Wang, Lele Zhang, Rubing Lin, Yuntao Wang, Pan Fan
Degeneration of intervertebral discs is considered one of the most important causes of low back pain and disability. The intervertebral disc (IVD) is characterized by its susceptibility to various stressors that accelerate the senescence and apoptosis of nucleus pulposus cells, resulting in the loss of these cells and dysfunction of the intervertebral disc. Therefore, how to reduce the loss of nucleus
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LRP8 promotes tumorigenesis in ovarian cancer through inhibiting p53 signaling Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-23 Yan Xu, Yang Zhou, Xiling Yi, Xiaocui Nie
Ovarian cancer (OC) is the most lethal gynecological malignancy with a high mortality rate. Low-density lipoprotein (LDL) receptor-related protein 8 (LRP8) is a cell membrane receptor belonging LDL receptor family and is involved in several tumor progressions. However, there is limited understanding of how LRP8 mediates OC development. LRP8 expression level was identified in human OC tissues and cells
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Overexpression of Fut 2, 4, and 8, and nuclear localization of Fut 4 in ovarian cancer cell lines induced by ascitic fluids from epithelial ovarian cancer patients Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-23 Nayely Paulina Alvear-Hernandez, Verónica Ivonne Hernández-Ramírez, Julio César Villegas-Pineda, Juan Carlos Osorio-Trujillo, José Jesús Guzmán-Mendoza, Dolores Gallardo-Rincón, Alfredo Toledo-Leyva, Patricia Talamás-Rohana
Fucosyltransferases (Fut) regulate the fucosylation process associated with tumorogenesis in different cancer types. Ascitic fluid (AF) from patients diagnosed with advanced stage of epithelial ovarian cancer (EOC) is considered as a dynamic tumor microenvironment associated with poor prognosis. Previous studies from our laboratory showed increased fucosylation in SKOV-3 and OVCAR-3, cancer-derived
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Endolysosomal two-pore channel 2 plays opposing roles in primary and metastatic malignant melanoma cells Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-23 Samantha Barbonari, Antonella D'Amore, Ali A. Hanbashi, Fioretta Palombi, Anna Riccioli, John Parrington, Antonio Filippini
The ion channel two-pore channel 2 (TPC2), localised on the membranes of acidic organelles such as endo-lysosomes and melanosomes, has been shown to play a role in pathologies including cancer, and it is differently expressed in primary versus metastatic melanoma cells. Whether TPC2 plays a pro- or anti-oncogenic role in different tumour conditions is a relevant open question which we have explored
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What makes SARS-CoV-2 unique? Focusing on the spike protein Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-23 Jingbo Qian, Shichang Zhang, Fang Wang, Jinming Li, Jiexin Zhang
Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) seriously threatens public health and safety. Genetic variants determine the expression of SARS-CoV-2 structural proteins, which are associated with enhanced transmissibility, enhanced virulence, and immune escape. Vaccination is encouraged as a public health intervention, and different types of vaccines are used worldwide. However
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A novel model for predicting prognosis in patients with idiopathic pulmonary fibrosis based on endoplasmic reticulum stress-related genes Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-18 Bin Liu, Xiang Zhang, Zikai Liu, Haihong Pan, Hongxu Yang, Qing Wu, Yan Lv, Tong Shen
Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease of unknown pathogenic origin. Endoplasmic reticulum (ER) stress refers to the process by which cells take measures to ER function when the morphology and function of the reticulum are changed. Recent studies have demonstrated that the ER was involved in the evolution and progression of IPF. In this study, we obtained transcriptome
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THBS1 promotes angiogenesis and accelerates ESCC malignant progression by the HIF-1/VEGF signaling pathway Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-17 Xiao Zhou, Qiaoxi Xia, Mantong Chen, Xiaona Zhang, Meihui Huang, Xiaoqi Zheng, Shaohong Wang, Bingli Wu, Zepeng Du
Previously, we demonstrated that the expression of THBS1 is increased in esophageal squamous cell carcinoma (ESCC) tissues and is correlated with lymph node metastasis and poor prognosis, indicating that THBS1 might be a candidate oncogene in ESCC. In this study, we future studied the specific role of THBS1 in ESCC and its molecular mechanism. Silencing THBS1 expression resulted in inhibition of cell
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The role of exo-miRNA in diagnosis and treatment of cancers, focusing on effective miRNAs in colorectal cancer Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-15 Hamzah H. Kzar Al-Shukri, Sana Abdul-Jabbar Ali, Karam Akram Al-Akkam, Ahmed Hjazi, Irodakhon Rasulova, Yasser Fakri Mustafa, Dahlia N. Al-Saidi, Mohammed Qasim Alasheqi, Ahmed Alawadi, Ali Alsaalamy
Small extracellular (EV) particles known as exosomes are released by a variety of cell types, including immune system cells, stem cells, and tumor cells. They are regarded as a subgroup of EVs and have a diameter that ranges from 30 to 150 nm. Proteins, lipids, nucleic acids (including RNA and DNA), and different bioactive compounds are among the wide range of biomolecules that make up the cargo of
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GLUT1 regulates the release of VEGF-A in the alveolar epithelium of lipopolysaccharide-induced acute lung injury Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-15 Yan Liang, Hailing Zhang, Jiahui Li, Xilong Wang, Jianpeng Xie, Yijian Li, Jiehong Li, Yunyao Qian, Haiyun Zhang, Tao Wang, Haixiong Tang, Xin Chen
Acute lung injury (ALI) is a severe disease with high mortality and poor prognosis, characterized by excessive and uncontrolled inflammatory response. Vascular endothelial growth factor A (VEGF-A) contributes to the development and progression of ALI. The aim of this study was to evaluate the role of glucose transporter 1 (GLUT1) in alveolar epithelial VEGF-A production in lipopolysaccharide (LPS)-induced
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Lipopolysaccharide-mediated ATP signaling regulates interleukin-6 mRNA expression via the P2-purinoceptor in human dental pulp cells Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-15 Ai Orimoto, Chiaki Kitamura, Kentaro Ono
Dental pulp cells play a crucial role in maintaining the balance of the pulp tissue. They actively respond to bacterial inflammation by producing proinflammatory cytokines, particularly interleukin-6 (IL-6). While many cell types release adenosine triphosphate (ATP) in response to various stimuli, the mechanisms and significance of ATP release in dental pulp cells under inflammatory conditions are
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Ginsenoside Rg3 overcomes tamoxifen resistance through inhibiting glycolysis in breast cancer cells Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-15 Wenhui Zhao, Jianli Ma, Qingyuan Zhang, Han Zhang, Wenjie Ma, Shuo Li, Ying piao, Shu Zhao, Shaochun Dai, Dabei Tang
Tamoxifen (TAM) resistance poses a significant clinical challenge in human breast cancer and exhibits high heterogeneity among different patients. Rg3, an original ginsenoside known to inhibit tumor growth, has shown potential for enhancing TAM sensitivity in breast cancer cells. However, the specific role and underlying mechanisms of Rg3 in this context remain unclear. Aerobic glycolysis, a metabolic
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Insights into cAMP-dependent molecular mechanisms regulating expression and function of LGALS16 gene in choriocarcinoma JEG-3 cells Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-11 Jennifer D. Kaminker, Ahmad G. Butt, Hailey Killeen, Alexander V. Timoshenko
The human choriocarcinoma cell line JEG-3 offers a valuable model to study galectin-16 gene (LGALS16) expression and functions in the context of placental cell differentiation and cancer cell biology. Recent evidence indicates that cAMP-mediated signaling pathways might be responsible for the upregulation of LGALS16; however, the underlying mechanisms are unknown. Here, we employed biochemical inhibitors
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A cell line derived from heart of rainbow trout is refractory to Tilapia lake virus Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-11 Amit Pande, Dimpal Thakuria, Basdeo Kushwaha, Ravindra Kumar, Murali S, Aakriti Rastogi, Neeraj Sood
Cell lines are important in vitro models to answer biological mechanisms with less genetic variations. The present study was attempted to develop a cell line from rainbow trout, where we obtained a cell line from the heart, named “RBT-H.” The cell line was authenticated using karyotyping and cytochrome c oxidase subunit I (COI) gene sequencing. The karyotype demonstrated diploid chromosome number (2n)
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Curcumin inhibits propofol-induced autophagy of MN9D cells via Akt/mTOR/p70S6K signaling pathway Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-09 Hongxia He, Yuping Han, Qiuyan Wan, Yao Yue, Shurong Li, Bingyin Su, Jun Li
The rapid rise in propofol dependency and abuse has highlighted limited resources for addressing substance abuse-related cognitive impairment, prompting the development of novel therapies. Dysregulated autophagy flow accelerates neuronal cell death, and interventions countering this dysregulation offer an appealing strategy for neuronal protection. Curcumin, a potent natural polyphenol derived from
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Knockdown of fat mass and obesity alleviates the ferroptosis in Parkinson's disease through m6A-NRF2-dependent manner Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-05 Pengfei Pang, Shirong Zhang, Xinxin Fan, Shitao Zhang
Emerging evidence has suggested that N6-methyladenosine (m6A) regulates the pathology of Parkinson's disease (PD). Nevertheless, the function of demethylase fat mass and obesity (FTO) associated pathogenesis is still not fully elucidated. Here, this research findings revealed that m6A-modification was decreased in PD models, meanwhile, the FTO level upregulated in the PD models. Functionally, in N
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Lipid droplets, autophagy, and ER stress as key (survival) pathways during ischemia-reperfusion of transplanted grafts Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-04 Daria Kamińska, Michał Skrzycki
Ischemia-reperfusion injury is an event concerning any organ under a procedure of transplantation. The early result of ischemia is hypoxia, which causes malfunction of mitochondria and decrease in cellular ATP. This leads to disruption of cellular metabolism. Reperfusion also results in cell damage due to reoxygenation and increased production of reactive oxygen species, and later by induced inflammation
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Bovine mammary epithelial cells can grow and express milk protein synthesis genes at reduced fetal bovine serum concentration Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-03 Zahra Sattari, Rikke Brødsgaard Kjærup, Martin Krøyer Rasmussen, Yuan Yue, Nina Aagaard Poulsen, Lotte Bach Larsen, Stig Purup
Milk proteins produced by lactating cells isolated from bovine mammary tissue can offer a sustainable solution to the high protein demand of a global growing population. Serum is commonly added to culture systems to provide compounds necessary for optimal growth and function of the cells. However, in a cellular agricultural context, its usage is desired to be decreased. This study aims at examining
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N6 methyladenosine eraser FTO suppresses Staphylococcus aureus-induced ferroptosis of bone marrow mesenchymal stem cells to ameliorate osteomyelitis through regulating the MDM2/TLR4/SLC7A11 signaling pathway Cell Biol. Int. (IF 3.9) Pub Date : 2024-01-02 Muguo Song, Kehan Lv, Zhi Xu, Junyi Li, Jian Sun, Jian Shi, Yongqing Xu
Osteomyelitis is a bone destructive inflammatory disease caused by infection. Ferroptosis is closely related to multiple inflammatory diseases, but the role of ferroptosis in Staphylococcus aureus (SA)-induced osteomyelitis remains unknown. In the present study, we found that SA treatment promoted the accumulation of iron, Fe2+, lipid peroxide, and malondialdehyde, increased TFRC and reduced FTH1 and
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Current stage of preclinical and clinical development of guggulsterone in cancers: Challenges and promises Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-26 Shumaila Ijaz, Javed Iqbal, Banzeer Ahsan Abbasi, Aasma Tufail, Tabassum Yaseen, Siraj Uddin, Kiran Usman, Rafi Ullah, Haseena Bibi, Palwasha Inam, Elvira Sagindykova, Eda Sönmez Gürer, Solomon Habtemariam, Daniela Calina, Javad Sharifi-Rad
Throughout human history, the utilization of medicinal herbs has been recognized as a crucial defense against various ailments, including cancer. Natural products with potential anticancer properties, capable of inducing apoptosis in cancer cells, have garnered substantial attention. One such agent under investigation is guggulsterone (GS), a phytosterol derived from the gum resin of the Commiphora
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KLK10 promotes the progression of KRAS mutant colorectal cancer via PAR1-PDK1-AKT signaling pathway Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-19 Kun Wu, Boyu Wu, Kangpeng Yan, Qunhua Ding, Zhiguo Miao
Kirsten rat sarcoma virus (KRAS) gene mutation is common in colorectal cancer (CRC) and is often predictive of treatment failure and poor prognosis. To understand the mechanism, we compared the transcriptome of CRC patients with wild-type and mutant KRAS and found that KRAS mutation is associated with the overexpression of a secreted serine protease, kallikrein-related peptidase 10 (KLK10). Moreover
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Deoxyribonuclease 1-like 3 inhibits colorectal malignancy through antagonizing NEDD4-triggered CDKN1A ubiquitination Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-18 Lifei Yu, Jin Huang
Deoxyribonuclease 1-like 3 (DNASE1L3) has been shown to play nonnegligible roles in several types of carcinomas. Nevertheless, the biological function, clinical relevance, and influence of DNASE1L3 in colorectal cancer (CRC) remain obscure. Immunohistochemistry was adopted to examine DNASE1L3 and CDKN1A expression in CRC tissue, and the clinical significance of DNASE1L3 was assessed. Cell counting
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HMGB1 in exosomes derived from gastric cancer cells induces M2-like macrophage polarization by inhibiting the NF-κB signaling pathway Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-17 Ke Liu, Hui Wang, Jumei Zhou, Suyu Zhu, Min Ma, Hua Xiao, Yi Ding
Gastric cancer (GC) seriously threatens human health. High mobility group protein B1 (HMGB1) and M2-like macrophages are closely associated with core events about human cancers, such as invasion, and metastasis, and cancer microenvironment. This study mainly determined the regulatory effect of HMGB1 in GC cell-derived exosomes on M2-like macrophage polarization as well as the underlying mechanism.
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Extracellular vesicles from oral mucosa stem cells promote lipid raft formation in human microglia through TLR4, P2X4R, and αVβ3/αVβ5 signaling pathways Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-15 Diana Romenskaja, Ugnė Jonavičė, Virginijus Tunaitis, Augustas Pivoriūnas
Targeting of disease-associated microglia represents a promising therapeutic approach that can be used for the prevention or slowing down neurodegeneration. In this regard, the use of extracellular vesicles (EVs) represents a promising therapeutic approach. However, the molecular mechanisms by which EVs regulate microglial responses remain poorly understood. In the present study, we used EVs derived
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14-3-3-η interacts with BCL-2 to protect human endothelial progenitor cells from ox-LDL-triggered damage Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-15 Hui Zhou, Xiaopei Sun, Yi Dai, Xiaotong Wang, Zhihong Dai, Xiuli Li
Oxidized low-density lipoprotein (ox-LDL) causes dysfunction of endothelial progenitor cells (EPCs), and we recently reported that 14-3-3-η can attenuate the damage triggered by ox-LDL in EPCs. However, the molecular mechanisms by which 14-3-3-η protects EPCs from the damage caused by ox-LDL are not fully understood. In this study, we observed that the expression of 14-3-3-η and BCL-2 were downregulated
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dCas9-guided demethylation of the AKT1 promoter improves milk protein synthesis in a bovine mastitis mammary gland epithelial model induced by using Staphylococcus aureus Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-15 Jie Liu, Xiangfei Wei, Yan Zhang, Yaoxiang Ran, Bo Qu, Chunmei Wang, Feng Zhao, Li Zhang
Mastitis is among the main factors affecting milk quality and yield. Although DNA methylation is associated with mastitis, its role in mastitis remains unclear. In this study, a bovine mastitis mammary epithelial cells (BMMECs) model was established via Staphylococcus aureus infection of bovine mammary gland epithelial cells (BMECs). Bisulfite sequencing PCR was used to determine the methylation status
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The potential role of stem cells-derived extracellular vesicles in the treatment of musculoskeletal system diseases Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-15 Zhuo Li, Demiao Hou, Zijin Tang, Lishun Xiong, Yiguo Yan
The therapeutic potential of stem cells-derived extracellular vesicles (EVs) has shown a great progress in the regenerative medicine. EVs are rich in a variety of bioactive substances, which are important carriers of signal transmission and interactions between cells, and they play an important role in the processes of tissue repair and regeneration. Several studies have shown that stem cells-derived
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Correction to “Pan-cancer analysis of the oncogenic role of HNRNPR in human tumors” Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-12
Yang, Y., Sun, J.-D., & Xiang, Z.-L. (2023). Pan-cancer analysis of the oncogenic role of HNRNPR in human tumors. Cell Biology International, 47, 1406−1426. https://doi.org/10.1002/cbin.12027 In the funding section, the grant number currently reads as “Science and Technology Commission of Shanghai Municipality (Grant No. 19DZ1930900).” It should be corrected to “Science and Technology Commission of
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The role of thrombomodulin in modulating ITGB3 expression and its implications for triple-negative breast cancer progression Cell Biol. Int. (IF 3.9) Pub Date : 2023-12-11 Yu-Jia Chang, G. M. Shazzad Hossain Prince, Po-Li Wei, Uyanga Batzorig, Chien-Yu Huang, Chin-Sheng Hung, Tung-Cheng Chang
Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer (BC) compared to other BC subtypes in clinical settings. Currently, there are no effective therapeutic strategies for TNBC treatment. Therefore, there is an urgent need to identify suitable biomarkers or therapeutic targets for TNBC patients. Thrombomodulin (TM) plays a role in cancer progression and metastasis in
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Positioning determines function: Wandering PKM2 performs different roles in tumor cells Cell Biol. Int. (IF 3.9) Pub Date : 2023-11-17 Shuo He, Yan Liang, Yiyi Tan, Qing Liu, Tao Liu, Xiaomei Lu, Shutao Zheng
Short for pyruvate kinase M2 subtype, PKM2 can be said of all-round player that is notoriously known for its metabolic involvement in glycolysis. Holding a dural role as a metabolic or non-metabolic (kinase) enzyme, PKM2 has drawn extensive attention over its biological roles implicated in tumor cells, including proliferation, migration, invasion, metabolism, and so on. wandering PKM2 can be transboundary
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Mesenchymal stem cells and their extracellular vesicles in urological cancers: Prostate, bladder, and kidney Cell Biol. Int. (IF 3.9) Pub Date : 2023-11-10 Mohamed J. Saadh, Hayaa M. Alhuthali, Oblitas Gonzales Aníbal, José Ander Asenjo-Alarcón, Dhuha Ghassan Younus, Ahmed Alhili, Zainab Hussein Adhab, Ohud Alsalmi, Amal F. Gharib, Renzon Daniel Cosme Pecho, Reza Akhavan-Sigari
Mesenchymal stem cells (MSCs) are recognized for their remarkable ability to differentiate into multiple cell types. They are also known to possess properties that can fight cancer, leading to attempts to modify MSCs for use in anticancer treatments. However, MSCs have also been found to participate in pathways that promote tumor growth. Many studies have been conducted to explore the potential of
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Isolation and propagation of bovine blood-derived macrophages using a mixed culture with bovine endothelial B46 cells Cell Biol. Int. (IF 3.9) Pub Date : 2023-11-03 Kanae Hiramatsu, Rina Ikeda, Satoko Kawaji, Yuichi Ueno, Reiko Nagata, Ken-Go Hayashi, Kosuke Iga, Miyako Yoshioka, Takato Takenouchi
Macrophages are innate immune cells with multiple functions such as phagocytosis, cytokine production, and antigen presentation. Since macrophages play critical roles in some bacterial infectious diseases in cattle, including tuberculosis, paratuberculosis, and brucellosis, the in vitro culturing of bovine macrophages is useful for evaluating host–pathogen interactions at the cellular and molecular
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Exosomes derived from uMSCs promote hair regrowth in alopecia areata through accelerating human hair follicular keratinocyte proliferation and migration Cell Biol. Int. (IF 3.9) Pub Date : 2023-11-03 Shouyi Hu, Jiayi Zhang, Qingqing Ji, Sujie Xie, Jingnuo Jiang, Haitao Ni, Xingying He, Yanlong Yang, Minjuan Wu
Alopecia areata (AA) is a complex genetic disease that results in hair loss due to an autoimmune-mediated attack on the hair follicle. Mesenchymal stem cells (MSCs) have great potential to induce hair regeneration due to their strong secretion ability and multidirectional differentiation. Recent studies have revealed that the therapeutic potential of MSCs comes from their secretion ability, which can
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Panax quinquefolium saponins attenuates microglia activation following acute cerebral ischemia-reperfusion injury via Nrf2/miR-103-3p/TANK pathway Cell Biol. Int. (IF 3.9) Pub Date : 2023-10-26 Xuesong Bai, Yan Qiu, Jian Wang, Yafen Dong, Tao Zhang, Hui Jin
Ischemic stroke is one of the leading causes of death and disability among adults worldwide. Intravenous thrombolysis is the only approved pharmacological treatment for acute ischemic stroke. However, reperfusion by thrombolysis will lead to the rapid activation of microglia cells which induces interferon-inflammatory response in the ischemic brain tissues. Panax quinquefolium saponins (PQS) has been
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Neosetophomone B induces apoptosis in multiple myeloma cells via targeting of AKT/SKP2 signaling pathway Cell Biol. Int. (IF 3.9) Pub Date : 2023-10-26 Shilpa Kuttikrishnan, Fareed Ahmad, Jericha M. Mateo, Kirti S. Prabhu, Tamam El-Elimat, Nicholas H. Oberlies, Cedric J. Pearce, Ammira S. Alshabeeb Akil, Ajaz A. Bhat, Feras Q. Alali, Shahab Uddin
Multiple myeloma (MM) is a hematologic malignancy associated with malignant plasma cell proliferation in the bone marrow. Despite the available treatments, drug resistance and adverse side effects pose significant challenges, underscoring the need for alternative therapeutic strategies. Natural products, like the fungal metabolite neosetophomone B (NSP-B), have emerged as potential therapeutic agents