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Flow Cytometry Protocol for GLUT4-myc Detection on Cell Surfaces. Biosci. Rep. (IF 4.0) Pub Date : 2024-03-27 Emilia Zanni-Ruiz, Luis S Mayorga, Martin Alejandro Pavarotti
Insulin and muscle contraction trigger GLUT4 translocation to the plasma membrane, which increases glucose uptake by muscle cells. Insulin resistance and type 2 diabetes are the result of impaired GLUT4 translocation. Quantifying GLUT4 translocation is essential for comprehending the intricacies of both physiological and pathophysiological processes involved in glucose metabolism. The most commonly
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Enzymatic activity of cGAS in the presence of three types of DNAs: Limited cGAS stimulation by single-stranded HIV-1 SL2 DNA. Biosci. Rep. (IF 4.0) Pub Date : 2024-03-26 Mineyuki Mizuguchi, Niko Kyan, Suzuka Nishimata, Yuko Nabeshima, Takayuki Obita
Cyclic GMP-AMP (cGAMP) synthase (cGAS) is activated by binding to DNA. Activated cGAS produces 2'3'-cGAMP, which subsequently binds to the adaptor protein STING (stimulator of interferon genes). This interaction triggers the cGAS/STING signaling pathway, leading to the production of type I interferons. Three types of DNA, namely double-stranded DNA longer than 40 base pairs, a 70-nucleotide single-stranded
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Prognostic and clinical significance of tumor-associated macrophages in esophageal squamous cell carcinoma after surgery:do biomarkers and distributions matter? Biosci. Rep. (IF 4.0) Pub Date : 2024-03-18 Bin Yi, Jun Zeng, Linfeng Li, Junjie Zhang, Yufan Chen, Yang Gao
The role of tumor-associated macrophages (TAMs) in patients with esophageal squamous cell carcinoma (ESCC) following surgery remains controversial. Hence, we performed this study to systematically analyze the prognostic and clinical significance of distinct TAMs biomarkers and distributions in ESCC patients underwent surgery. Methods: PubMed, Web of Science, and EMBASE databases were searched up to
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The Molecular Sociology of NHERF1 PDZ Proteins Controlling Renal Hormone-Regulated Phosphate Transport. Biosci. Rep. (IF 4.0) Pub Date : 2024-03-11 Tatyana Mamonova, Peter A Friedman
Parathyroid hormone (PTH) and fibroblast growth factor-23 (FGF23) control extracellular phosphate levels by regulating renal NPT2A-mediated phosphate transport by a process requiring the PDZ scaffold protein NHERF1. NHERF1 possesses two PDZ domains, PDZ1 and PDZ2, with identical core-binding GYGF motifs explicitly recognizing distinct binding partners that play different and specific roles in hormone-regulated
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Detection of hepatocellular carcinoma methylation markers in salivary DNA. Biosci. Rep. (IF 4.0) Pub Date : 2024-03-08 Catherine Mezzacappa, Zhangwei Wang, Lingeng Lu, Harvey Risch, Tamar Taddei, Herbert Yu
Background Alterations to DNA methylation have been identified in both hepatocellular carcinoma (HCC) tumor and circulating DNA from affected individuals. These markers have potential utility in HCC screening. Adherence to HCC screening is poor and acceptable HCC screening tests are needed. Methods A feasibility study was performed on a subset of case patients and control subjects from a prior study
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Eosinophil extracellular traps in eosinophilic chronic rhinosinusitis induce Charcot-Leyden crystal formation and eosinophil recruitment. Biosci. Rep. (IF 4.0) Pub Date : 2024-3-1 Siyuan Zhang, Zhenlin Wang
Eosinophil extracellular traps (EETs) are implicated in various eosinophil-associated diseases; however, their role in chronic rhinosinusitis (CRS) remains unclear. In the present study, 57 CRS patients were enrolled, and immunofluorescence was used to analyze EETs in eosinophilic (eCRS) and non-eosinophilic (Non-eCRS) tissues. MSD was used to examine IL-4, IL-5, and IL-13 concentrations in tissue
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Elucidating the role of intrinsic adenosine A1 receptors in acute alcoholism using human induced pluripotent stem cell-derived hepatocytes. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-28 Takako Nagata, Yuning George Huang
Acute alcoholic hepatitis (AAH) from binge drinking is a serious disease. It is associated with a high mortality rate, especially among young adults. Apoptosis is known to be a primary cause of liver damage, and it can be induced by either intrinsic signaling pathways or by reactive oxygen species (ROS). Adenosine A1 receptors (ADORA1) are known to be involved in ethanol metabolism; however, underlying
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Integrative analysis of metabolism subtypes and identification of prognostic metabolism-related genes for glioblastoma. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-28 Jiahui Li, Yutian Wei, Shupeng Cheng, Jiali Liu, Xia Zhang, Huaide Qiu, Jianan Li, Chuan He
Increasing evidence has demonstrated that cancer cell metabolism is a critical factor in tumor development and progression; however, its role in glioblastoma (GBM) remains limited. In this study, we classified GBM into three metabolism subtypes (MC1, MC2, and MC3) through cluster analysis of 153 GBM samples from the RNA-sequencing data of The Cancer Genome Atlas (TCGA) based on 2752 metabolism-related
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Progesterone inhibits endometrial cancer growth by inhibiting glutamine metabolism through ASCT2. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-28 Jinqiu Guo, Jianhui Fan, Yaru Zhang, Mengyue Li, Zeen Jin, Yuhong Shang, Hongshuo Zhang, Ying Kong
Endometrial carcinoma (EC) is a common malignancy which originates from the endometrium and grows in female reproductive system. Surgeries, as current treatment for the cancer, however, cannot meet the fertility needs of young women patients. Thus, progesterone (P4) therapy is indispensable due to its effective temporary preservation of female fertility. Many cancer cells are often accompanied by changes
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Unearthing the Role of Septins in Viral Infections. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-19 Jasmine Elanie Khairat, Muhammad Nur Adam Hatta, Nurshariza Abdullah, Adzzie Shazleen Azman, Shee Yin Ming Calvin, Sharifah Syed Hassan
Septin proteins are a subfamily of closely related GTP-binding proteins conserved in all species except for higher plants and perform essential biological processes. Septins self-assemble into heptameric or octameric complexes and form higher-order structures such as filaments, rings, or gauzes by end-to-end binding. Their close association with cell membrane components makes them central in regulating
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New insights into GATOR2-dependent interactions and its conformational changes in amino acid sensing. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-19 Can Yang, Xuan Sun, Geng Wu
Eukaryotic cells coordinate growth under different environmental conditions via mechanistic target of rapamycin complex 1 (mTORC1). In the amino-acid-sensing signaling pathway, the GATOR2 complex, containing five evolutionarily conserved subunits (WDR59, Mios, WDR24, Seh1L and Sec13), is required to regulate mTORC1 activity by interacting with upstream CASTOR1 (arginine sensor) and Sestrin2 (leucine
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METTL3 promotes osteogenic differentiation of human umbilical cord mesenchymal stem cells by upregulating m6A modification of circCTTN. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-15 Shujiang Chen, Xiaoqiong Duan, Yanjing He, Wenchuan Chen
Background Human umbilical cord mesenchymal stromal cells (hUCMSCs) are promising seed cells in bone tissue engineering. circRNA and N6-methyladenosine (m6A) RNA methylation play important roles in osteogenic differentiation. Here, we investigated the potential relevance of a critical circRNA, hsa_circ_0003376 (circCTTN), and methyltransferase-like 3 (METTL3) in osteogenic differentiation of hUCMSCs
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Exploring the Potential of VGLL3 Methylation as a Prognostic Indicator for Intracranial Aneurysm, with Gender-specific Considerations. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-13 Yuchun Liu, Siqi Chen, Enhao Zhang, Yinbin Xu, Xinpeng Deng, Ziliang Hu, Sheng Nie, Yinglu Lin, Yi Huang
DNA methylation is widely recognized to play a role in intracranial aneurysm (IA) pathogenesis. We investigated the levels of methylation of vestigial-like 3 (VGLL3) in IA, and explored its potential as a prognostic indicator. A total of 48 patients with IA and 48 healthy controls were included in this study. Methylation levels of CpG sites were assessed using bisulfite pyrosequencing, and levels of
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Seed longevity and genome damage. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-07 Wanda Waterworth, Atheer Balobaid, Chris West
Seeds are the mode of propagation for most plant species and form the basis of both agriculture and ecosystems. Desiccation tolerant seeds, representative of most crop species, can survive maturation drying to become metabolically quiescent. The desiccated state prolongs embryo viability and provides protection from adverse environmental conditions, including seasonal periods of drought and freezing
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TCR beta Chain Repertoire Characteristic between Healthy Human CD4+ and CD8+ T Cells. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-07 Ge Li, Yaqiong Chen, Yinji Liu, Zhenfang Gao, Ruiyan Jia, Zhonglin Lv, Yuxiang Li, Zhiding Wang, Gencheng Han
T cell is vital in the adaptive immune system, which relays on TCR to recognize and defend against infection and tumors. T cells are mainly divided into well-known CD4+ and CD8+ T cells, which can recognize short peptide antigens presented by MHC class II and MHC class I respectively in humoral and cell-mediated immunity. Due to the HLA diversity and restriction with peptides complexation, TCRs are
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Immune Receptors and Aging Brain. Biosci. Rep. (IF 4.0) Pub Date : 2024-02-01 Maja Djurisic
Aging brings about a myriad of degenerative processes throughout the body. A decrease in cognitive abilities is one of the hallmark phenotypes of aging, underpinned by neuroinflammation and neurodegeneration occurring in the brain. This review focuses on the role of different immune receptors expressed in cells of the central and peripheral nervous systems. We will discuss how immune receptors in the
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Identification and validation of STAT4 as a prognostic biomarker in acute myeloid leukemia. Biosci. Rep. (IF 4.0) Pub Date : 2024-01-31 Chenyu Li, Jingyuan Zhao, Bingyu Kang, Shuai Li, Jingya Tang, Deshi Dong, Yanwei Chen
Acute myelogenous leukemia (AML) is a common malignancy and is supposed to have the ability to escape host immune surveillance. This study aimed to identify key genes in AML that may affect tumor immunity and to provide prognosis biomarkers of AML. The Cancer Genome Atlas (TCGA) dataset was screened for transcription factors (TFs) involved in immunity and influencing survival, combining Gene Expression
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Excitation energy transfer in proteoliposomes reconstituted with LH2 and RC-LH1 complexes from Rhodobacter sphaeroides. Biosci. Rep. (IF 4.0) Pub Date : 2024-01-16 Xia Huang, Cvetelin Vasilev, David Jk Swainsbury, C Neil Hunter
Light-harvesting 2 (LH2) and reaction-centre light-harvesting 1 (RC-LH1) complexes purified from the photosynthetic bacterium Rhodobacter (Rba.) sphaeroides were reconstituted into proteoliposomes either separately, or together at three different LH2:RC-LH1 ratios, for excitation energy transfer studies. Atomic force microscopy (AFM) was used to investigate the distribution and association of the complexes
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Metabolic and behavioral alterations associated with viral vector-mediated toxicity in the paraventricular hypothalamic nucleus. Biosci. Rep. (IF 4.0) Pub Date : 2024-01-16 Rohan Savani, Erin Park, Nidhi Busannagari, Yi Lu, Hyokjoon Kwon, Le Wang, Zhiping P Pang
Combining adeno-associated virus (AAV)-mediated expression of Cre recombinase with genetically modified floxed animals is a powerful approach for assaying the functional role of genes in regulating behavior and metabolism. Extensive research in diverse cell types and tissues using AAV-Cre has shown it can save time and avoid developmental compensation as compared to using Cre driver mouse line crossings
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Lipid-based Nutritional Supplement Impact on Energy Intake, Appetite, Glucose and Insulin Levels in Under-Weight Pregnant and Lactating Women with Preeclampsia. Biosci. Rep. (IF 4.0) Pub Date : 2024-01-10 Nabila Sher Mohammad, Rubina Nazli, Sadia Fatima, Fozia Fozia, Hafza Zafar, Mashal Zafar, Zarghuna Zafar, Warda Khan, Mahmoud M A Abulmeaty, Dara Aldisi, Juan E Andrade Laborde, Mourad A M Aboul-Soud
To investigate the response of nutritional supplement (LNS-PLW) on appetite score, energy intake, insulin and glucose levels in preeclamptic women.
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A Promise for Neuronal Repair: Reprogramming Astrocytes into Neurons in Vivo. Biosci. Rep. (IF 4.0) Pub Date : 2024-01-04 Lijuan Huang, Xinyu Lai, Xiaojun Liang, Jiafeng Chen, Yue Yang, Wei Xu, Qingchun Qin, Rongxing Qin, Xiaoying Huang, Minshan Xie, Li Chen
Massive loss of neurons following brain injury or disease is the primary cause of central nervous system dysfunction. Recently, much research has been conducted on how to compensate for neuronal loss in damaged parts of the nervous system and thus restore functional connectivity among neurons. Direct somatic cell differentiation into neurons using pro-neural transcription factors, small molecules,
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SARS-CoV-2 NSP14 MTase activity is critical for inducing canonical NF-κB activation. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-22 Marie J Tofaute, Benjamin Weller, Carina Graß, Hridi Halder, Bushra Dohai, Pascal Falter-Braun, Daniel Krappmann
Upon SARS-CoV-2 infection, patients with severe forms of COVID-19 often suffer from a dysregulated immune response and hyperinflammation. Aberrant expression of cytokines and chemokines is associated with strong activation of the immunoregulatory transcription factor NF-κB, which can be directly induced by the SARS-CoV-2 protein NSP14. Here, we use NSP14 mutants and generated cells with host factor
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Biological ammonium transporters from the Amt/Mep/Rh superfamily: mechanism, energetics, and technical limitations. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-22 Gordon Williamson, Adriana Bizior, Thomas Harris, Leighton Pritchard, Paul Alan Hoskisson, Arnaud Javelle
The exchange of ammonium across cellular membranes is a fundamental process in all domains of life and is facilitated by the ubiquitous Amt/Mep/Rh transporter superfamily. Remarkably, despite a high structural conservation in all domains of life, these proteins have gained various biological functions during evolution. It is tempting to hypothesise that the physiological functions gained by these proteins
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Metabolic hormones are integral regulators of female reproductive health and function. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-22 Faria Athar, Muskan Karmani, Nicole M Templeman
The female reproductive system is strongly influenced by nutrition and energy balance. It is well known that food restriction or energy depletion can induce suppression of reproductive processes, while overnutrition is associated with reproductive dysfunction. However, the intricate mechanisms through which nutritional inputs and metabolic health are integrated into the coordination of reproduction
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Biological and mutational analyses of CXCR4-antagonist interactions and design of new antagonistic analogs. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-22 Qian Meng, Ruohan Zhu, Yujia Mao, Siyu Zhu, Yi Wu, Lina S M Huang, Aaron Ciechanover, Jing An, Yan Xu, Ziwei Huang
The chemokine receptor CXCR4 has become an attractive therapeutic target for HIV-1 infection, hematopoietic stem cell mobilization, and cancer metastasis. A wide variety of synthetic antagonists of CXCR4 have been developed and studied for a growing list of clinical applications. To compare the biological effects of different antagonists on CXCR4 functions and their common and/or distinctive molecular
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A low-cost, low-input method establishment for m6A MeRIP-seq. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-19 Wenjuan Xia, Ling Guo, Huapeng Su, Jincheng Li, Jiafeng Lu, Hong Li, Boxian Huang
N6-methyladenosine (m6A) is a highly prevalent modification found in mammal mRNA molecules that plays a crucial role in the regulation of cellular function. m6A RNA immunoprecipitation sequencing (MeRIP-seq) has been frequently used in transcriptomics research to identify the location of m6A. MABE572 (Millipore) is the most widely utilized and efficient anti-m6A antibody for MeRIP-seq. However, due
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The presence of a G-quadruplex prone sequence upstream of a minimal promoter increases transcriptional activity in the yeast Saccharomyces cerevisiae. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-19 Libuše Kratochvilová, Matúš Vojsovič, Natália Valková, Lucie Šislerová, Zeinab El Rashed, Alberto Inga, Paola Monti, Václav Brázda
Non-canonical secondary structures in DNA are increasingly being revealed as critical players in DNA metabolism, including modulating the accessibility and activity of promoters. These structures comprise the so-called G-quadruplexes (G4s) that are formed from sequences rich in guanine bases. Using a well-defined transcriptional reporter system, we sought to systematically investigate the impact of
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Study on the production of Sophorolipid by Starmerella bombicola yeast using waste fried oil fermentation. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-08 Haifeng Wang, Ruifang Gao, Xin Song, Xiangdong Yuan, Xiuli Chen, Yanling Zhao
Sophorolipid (SLs) are surface active compounds that have excellent surface-lowering properties. SLs was produced by Starmerella bombicola (CGMCC1576) yeast with sunflower seeds oil, fried waste oil, cooked tung oil and raw tung oil used as hydrophobic carbon sources. The results showed that the strain could use sunflower seed oil and fried waste oil as hydrophobic carbon sources to produce SLs, and
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Structural and Functional Coupling in Crosslinking Uracil-DNA Glycosylase UDGX. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-07 Chuan Liang, Ye Yang, Ping Ning, Chenyan Chang, Weiguo Cao
Enzymes in uracil-DNA glycosylase (UDG) superfamily are involved in removal of deaminated nucleobases such as uracil, methylcytosine derivatives such as formylcytosine and carboxylcytosine, and other base damage in DNA repair. UDGX is the latest addition of a new class to the UDG superfamily with a sporadic distribution in bacteria. UDGX type enzymes have a distinct biochemical property of crosslinking
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Melanogrit potentiates melanogenesis by escalating cellular tyrosinase activity and MITF levels via pERK inhibition. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-06 Acharya Balkrishna, Savita Lochab, Sudeep Verma, Jyotish Srivastava, Rishabh Dev, Anurag Varshney
Vitiligo is characterized by the development of white patches on the skin either due to the loss of functional melanocytes or perturbations in the melanogenesis pathway. In this study, we investigated the therapeutic potential of herbo-mineral formulation, Melanogrit in neutralizing the white patches in the skin. The study utilized UPLC/MS-QToF technique to determine the diversified phytochemical profile
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The differentiation courses of the Tfh cells: a new perspective on autoimmune disease pathogenesis and treatment. Biosci. Rep. (IF 4.0) Pub Date : 2023-12-05 Qingya Yang, Fang Zhang, Hongyi Chen, Yuman Hu, Ning Yang, Wenyan Yang, Jing Wang, Yaxu Yang, Ran Xu, Chao Xu
The follicular helper T cells are derived from CD4+T cells, promoting the formation of germinal centers and assisting B cells to produce antibodies. This review describes the differentiation process of Tfh cells from the perspectives of the initiation, maturation, migration, efficacy, and subset classification of Tfh cells, and correlates it with autoimmune disease, to provide information for researchers
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Retraction: SMARCB1 expression is a novel diagnostic and prognostic biomarker for osteosarcoma. Biosci. Rep. (IF 4.0) Pub Date : 2023-11-30
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MIR938 rs2505901 T>C polymorphism is associated with increased neuroblastoma risk in Chinese children. Biosci. Rep. (IF 4.0) Pub Date : 2023-11-30 Susu Jiang,Xiuhong Sun,Xinxin Zhang,Chunlei Zhou,Haiyan Wu,Jing He,Wenhan Yang
Neuroblastoma (NB) is a kind of childhood cancer that is a prevailing and deadly solid neoplasm among pediatric malignancies. The transcriptional output of MIR938 is capable of participating in the posttranscriptional modulation of gene expression, whereby it exerts its regulatory effect by modulating both the stability and translation of target mRNAs. Previous studies showed that MIR938 was associated
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Therapeutic activity of green synthesized selenium nanoparticles from turmeric against cisplatin-induced oxido-inflammatory stress and cell death in mice kidney. Biosci. Rep. (IF 4.0) Pub Date : 2023-11-30 Barakat M ALRashdi,Roaya A Mohamed,Amal H Mohamed,Feryal A Samoul,Mazen I Mohamed,Mohsen M Moussa,Saad M Alrashidi,Bassel Dawod,Ola A Habotta,Ahmed E Abdel Moneim,Shimaa S Ramadan
Cisplatin (CDDP) is a commonly prescribed chemotherapeutic agent; however, its associated nephrotoxicity limits its clinical efficacy and sometimes requires discontinuation of its use. The existing study was designed to explore the reno-therapeutic efficacy of turmeric (Tur) alone or conjugated with selenium nanoparticles (Tur-SeNPs) against CDDP-mediated renal impairment in mice and the mechanisms
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Characterisation and functionalisation of chitosan nanoparticles as carriers for double-stranded RNA (dsRNA) molecules towards sustainable crop protection. Biosci. Rep. (IF 4.0) Pub Date : 2023-11-30 Dora Scarpin,Luca Nerva,Walter Chitarra,Loredana Moffa,Francesca D'Este,Marco Vuerich,Antonio Filippi,Enrico Braidot,Elisa Petrussa
The need to minimise the impact of phytosanitary treatments for disease control boosted researchers to implement techniques with less environmental impact. The development of technologies using molecular mechanisms based on the modulation of metabolism by short dsRNA sequences appears promising. The intrinsic fragility of polynucleotides and the high cost of these techniques can be circumvented by
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Alteration in cartilage matrix stiffness as an indicator and modulator of osteoarthritis. Biosci. Rep. (IF 4.0) Pub Date : 2023-11-27 Jing Song,Xuemin Zeng,Chenzhi Li,Hongyan Yin,Sui Mao,Dapeng Ren
Osteoarthritis (OA) is characterized by cartilage degeneration and destruction, leading to joint ankylosis and disability. The major challenge in diagnosing OA at early stage is not only lack of clinical symptoms but also the insufficient histological and immunohistochemical signs. Alteration in cartilage stiffness during OA progression, especially at OA initiation, has been confirmed by growing evidences
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Dexamethasone Prophylaxis Protects from Acute High-Altitude Illness by Modifying the Peripheral Blood Mononuclear Cell Inflammatory Transcriptome. Biosci. Rep. (IF 4.0) Pub Date : 2023-11-17 Rahul Kumar, Neha Chanana, Kavita Sharma, Tsering Palmo, Michael H Lee, Aastha Mishra, Kevin Nolan, Dara C Fonseca Balladares, Claudia Mickael, Mohit D Gupta, Tashi Thinlas, Qadar Pasha, Brian B Graham
Acute high-altitude (HA) exposure can induce several pathologies. Dexamethasone (DEX) can be taken prophylactically to prevent HA disease, but the mechanism by which it acts in this setting is unclear. We studied the transcriptome of peripheral blood mononuclear cells (PBMCs) from 16 subjects at low altitude (LA, 225m) and then 3 days after acute travel to HA (3500m) during the India-Leh-Dexametha
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Commentary on "Increased stiffness of omental arteries from late pregnant women at advanced maternal age". Biosci. Rep. (IF 4.0) Pub Date : 2023-11-17 Anna E Stanhewicz, Kelsey S Schwartz, Ruda Lee
Worldwide, pregnancy at age 35 or older, termed "advanced maternal age", is increasing exponentially. As the incidence of pregnancy at advanced maternal age has increased, a growing body of evidence has suggested that advanced maternal age is also associated with increased risk for adverse maternal and fetal outcomes outside of genetic anomalies. Importantly, despite the mounting evidence and the increased
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Inhibition of smooth muscle cell death by Angiotensin 1-7 protects against abdominal aortic aneurysm. Biosci. Rep. (IF 4.0) Pub Date : 2023-11-10 Anshul S Jadli, Karina P Gomes, Noura N Ballasy, Tishani Methsala Wijesuriya, Darrell Belke, Paul W M Fedak, Vaibhav B Patel
Abdominal aortic aneurysm (AAA) represents a debilitating vascular disease characterized by aortic dilatation and wall rupture if it remains untreated. We aimed to determine the effects of Ang 1-7 in a murine model of AAA and to investigate the molecular mechanisms involved. 8-10 weeks old apolipoprotein E-deficient mice (ApoEKO) were infused with Ang II (1.44 mg/kg/day, s.c.) and treated with Ang
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Melatonin targets ferroptosis through bimodal alteration of redox environment and cellular pathways in NAFLD model. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-31 Moumita Saha,Sanjib Das,Krishnendu Manna,Krishna Das Saha
Ferroptosis is a non-conventional cellular death caused by lipid peroxide induced iron deposition. Intracellular lipid accumulation followed by generation of lipid peroxides is an hallmark of non-alcoholic fatty liver disease (NAFLD). Melatonin (MLT) is an important pineal hormone with tremendous antioxidant and anti-inflammatory properties. Various studies targeted ferroptosis in different diseases
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Implications of ubiquitination and the maintenance of replication fork stability in cancer therapy. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-31 Donghui Xia,Xuefei Zhu,Ying Wang,Peng Gong,Hong-Shu Su,Xingzhi Xu
DNA replication forks are subject to intricate surveillance and strict regulation by sophisticated cellular machinery. Such close regulation is necessary to ensure the accurate duplication of genetic information and to tackle the diverse endogenous and exogenous stresses that impede this process. Stalled replication forks are vulnerable to collapse, which is a major cause of genomic instability and
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Diagnostic significance of alanine aminotransferase isoenzymes in alcoholic and non-alcoholic fatty liver cancers. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-26 Dandan Zhou, Zuowei Yuan, Xiaoqin Shu, Hejun Tang, Jie Li, Yangmin Ye, Nana Tao, Fangzhu Zhou, Jun Zhang, Jian Zheng, Qian Wu, Juan Zhang
Alanine aminotransferase (ALT) expression is highly elevated in the serum of patients with hepatocellular carcinoma. However, the role of ALT isoenzymes in the total ALT activity remains unclear. In this study, we systematically investigated the role of ALT isoenzymes in alcoholic and non-alcoholic fatty liver cancer. Materials and methods: The expression of ALT1 and ALT2 at the mRNA and protein levels
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Branched-chain fatty acids affect the expression of fatty acid synthase and C-reactive protein genes in the hepatocyte cell line. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-18 Paulina Gozdzik, Aleksandra Czumaj, Tomasz Sledzinski, Adriana Mika
Fatty acids (FAs) are known to play an important role in human metabolism; however, still little is known about the functions of certain FA classes present in blood at relatively low concentrations. Examples of such compounds include branched-chain fatty acids (BCFAs). Recently, lowered BCFAs blood concentration was noticed in obese patients. An inverse correlation was found between serum concentrations
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Comparative genomics hints at dispensability of multiple essential genes in two E. coli L-form strains. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-11 Yunfei Liu, Yueyue Zhang, Chen Kang, Di Tian, Hui Lu, Bo-Ying Xu, Yang Xia, Akiko Kashiwagi, Martin Westermann, Christian Hoischen, Jian Xu, Tetsuya Yomo
Despite the critical role of bacterial cell walls in maintaining cell shapes, certain environmental stressors can induce the transition of many bacterial species into a wall-deficient state called L-form. Long-term induced Escherichia coli L-forms lose their rod shape and usually hold significant mutations that affect cell division and growth. Besides this, the genetic background of L-form bacteria
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Assessing the dynamics and macromolecular interactions of the intrinsically disordered protein YY1. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-10 Heather L Donald, Ashleigh A Blane, Sindisiwe Buthelezi, Previn Naicker, Stoyan Stoychev, Jacob Majakwara, Sylvia Fanucchi
YY1 is a ubiquitously expressed, intrinsically disordered transcription factor involved in neural development. The oligomeric state of YY1 varies depending on the environment. These structural changes may alter its DNA binding ability and hence its transcriptional activity. Just as YY1's oligomeric state can impact its role in transcription, so does its interaction with other proteins such as FOXP2
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Regulatory T cells in lung disease and transplantation. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-05 Peizhen Lao, Jingyi Chen, Longqian Tang, Jiwen Zhang, Yuxi Chen, Yuyin Fang, Xingliang Fan
Pulmonary disease can refer to the disease of the lung itself or the pulmonary manifestations of systemic diseases, which are often connected to the malfunction of the immune system. Regulatory T (Treg) cells have been shown to be important in maintaining immune homeostasis and preventing inflammatory damage, including lung diseases. Given the increasing amount of evidence linking Treg cells to various
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Cell organelles are retained inside pyroptotic corpses during inflammatory cell death. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-05 Anne Hempel, Andrea D'Osualdo, Scott Joseph Snipas, Guy S Salvesen
Many proinflammatory proteins are released via the necrotic form of cell death known as pyroptosis. Sometimes known as gasdermin D (GSDMD) dependent cell death, pyroptosis results from the formation of pores in the plasma membrane leading to eventual cell lysis. Seeking to understand the magnitude of this cell lysis we measured the size of proteins released during pyroptosis. We demonstrate that there
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GLUT4 Dispersal at the Plasma Membrane of Adipocytes: A Super-Resolved Journey. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-04 Angéline Geiser, Shannan Foylan, Peter Tinning, Nia Bryant, Gwyn W Gould
In adipose tissue, insulin stimulates glucose uptake by mediating the translocation of GLUT4 from intracellular vesicles to the plasma membrane. In 2010, insulin was revealed to also have a fundamental impact on the spatial distribution of GLUT4 within the plasma membrane, with the existence of two GLUT4 populations at the plasma membrane being defined: 1) as stationary clusters and 2) as diffusible
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Writers and Readers of H3K9me2 Form Distinct Protein Networks During the Cell Cycle, Which Include Candidates for H3K9 Mimicry. Biosci. Rep. (IF 4.0) Pub Date : 2023-10-02 Gareth Pollin, Thiago M De Assuncao, Salomao Doria Jorge, Young-In Chi, M Cristine Charlesworth, Benjamin Madden, Juan L Iovanna, Michael T Zimmermann, Raul Urrutia, Gwen Lomberk
Histone H3 lysine 9 methylation (H3K9me), which is written by the Euchromatic Histone Lysine Methyltransferases EHMT1 and EHMT2 and read by the heterochromatin protein 1 (HP1) chromobox (CBX) protein family, is dysregulated in many types of cancers. Approaches to inhibit regulators of this pathway are currently being evaluated for therapeutic purposes. Thus, knowledge of the complexes supporting the
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Commentary on: The actin bundling activity of ITPKA mainly accounts for its migration-promoting effect in lung cancer cells. Biosci. Rep. (IF 4.0) Pub Date : 2023-09-27 Xin Zhang,Jiadi He,Dong Ren
1,4,5-triphosphate 3-kinase A (ITPKA) was first described and characterized by Irvine et al. in 1986 and cloned by Takazawa et al. in 1990. It is one of the components of the Ca2+ and calmodulin signaling pathway and a substrate for cAMP-dependent kinase (PKA) and protein kinase C (PKC), and is mainly involved in the regulation of intracellular inositol polyphosphate signaling molecules. Through a
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Prognostic value and immune landscapes of immunogenic cell death-related lncRNAs in hepatocellular carcinoma. Biosci. Rep. (IF 4.0) Pub Date : 2023-09-27 Wanying Chen,Kexin Shu,Chenxi Cai,Jiatong Ding,Xin Zhang,Wenxiong Zhang,Kang Wang
BACKGROUND Both immunogenic cell death (ICD) and long noncoding RNAs (lncRNAs) are strongly associated with tumor development, but the mechanism of action of ICD-associated lncRNAs in hepatocellular carcinoma (HCC) remains unclear. METHODS We collected data from 365 HCC patients from The Cancer Genome Atlas (TCGA) database. We formulated a prognostic signature of ICD-associated lncRNAs and a nomogram
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MiR-21 attenuates FAS-mediated cardiomyocyte apoptosis by regulating HIPK3 expression. Biosci. Rep. (IF 4.0) Pub Date : 2023-09-27 Xinyu Wang,Tingting Zhang,Jianlong Zhai,Zhongli Wang,Yan Wang,Lili He,Sai Ma,Hanying Xing,Yifang Guo
MicroRNA-21 (miR-21) plays an anti-apoptotic role following ischemia-reperfusion (I/R) injury (IRI) in vivo; however, its underlying mechanism remains unclear. The present study explored the effects of miR-21 and homeodomain interacting protein kinase 3 (HIPK3) on cardiomyocyte apoptosis induced by hypoxia/reoxygenation (H/R) in vitro. To this end, the rat cardiomyocyte H9C2 cell line was exposed to
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Involvement of DPP3 in modulating oncological features and oxidative stress response in esophageal squamous cell carcinoma. Biosci. Rep. (IF 4.0) Pub Date : 2023-09-27 Mohit Arora,Sarita Kumari,Lokesh Kadian,Geethadevi Anupa,Jay Singh,Anurag Kumar,Deepika Verma,Raja Pramanik,Sunil Kumar,Rajni Yadav,Anita Chopra,Shyam S Chauhan
Resistance to therapy in esophageal squamous cell carcinoma (ESCC) is a critical clinical problem and identification of novel therapeutic targets is highly warranted. Dipeptidyl peptidase III (DPP3) is a zinc-dependent aminopeptidase and functions in the terminal stages of the protein turnover. Several studies have reported overexpression and oncogenic functions of DPP3 in numerous malignancies. The
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Bioinformatics identification and integrative analysis of ferroptosis-related key lncRNAs in patients with osteoarthritis. Biosci. Rep. (IF 4.0) Pub Date : 2023-9-13 Tengyun Yang, Guang Yang, Guoliang Wang, Di Jia, Bohan Xiong, Xiaojun Lu, Yanlin Li
Ferroptosis and dysregulation of long non-coding RNA (lncRNA) have been described to be strictly relevant to the pathogenesis of osteoarthritis (OA). However, the connection between ferroptosis and lncRNA in OA is poorly appreciated. Herein, we investigated the functional contribution of lncRNA markers correlated with the progression of human OA by comprehensive bioinformatics analysis of a panoramic
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miRNA-423 rs6505162 and miRNA-6811 rs2292879 SNP Associated with Lung Cancer in Hainan, China. Biosci. Rep. (IF 4.0) Pub Date : 2023-09-11 Jing Zhou, Chong Meng, Yixuan Li, Yihui Fu, Wenfang Long, Hairong Huang, Yunru Liu, Pengfei Lyu, Sha Xiao
MicroRNAs (miRNAs) are known to exert significant influence on various physiological processes and diseases, including cancers. The primary objective of this present study was to examine the impact of eight single nucleotide polymorphisms (SNPs) in miRNA on the susceptibility to lung cancer (LC) within the Chinese Southern population.
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DNA Damage and Repair Dependencies of Ionising Radiation Modalities. Biosci. Rep. (IF 4.0) Pub Date : 2023-09-11 Emma Melia, Jason Parsons
Radiotherapy is utilised in the treatment of ~50 % of all human cancers, which predominantly employs photon radiation. However, particle radiotherapy elicits significant benefits over conventional photons due to more precise dose deposition and increased linear energy transfer (LET) that generates an enhanced therapeutic response. Specifically, proton beam therapy (PBT) and carbon ion therapy (CIRT)
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Intravenous ferric carboxymaltose and ferric derisomaltose alter the intestinal microbiome in female iron-deficient anemic mice. Biosci. Rep. (IF 4.0) Pub Date : 2023-09-06 Timo Rieg, Jianxiang Xue, Monica Stevens, Linto Thomas, James Robert White, Jessica A Dominguez Rieg
Iron deficiency anemia (IDA) is a leading global health concern affecting approximately 30% of the population. Treatment for IDA consists of replenishment of iron stores, either by oral or intravenous (IV) supplementation. There is a complex bidirectional interplay between the gut microbiota, the host's iron status, and dietary iron availability. Dietary iron deficiency and supplementation can influence
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Gut microbiota profile and the influence of nutritional status on bacterial distribution in diabetic and healthy Tunisian subjects. Biosci. Rep. (IF 4.0) Pub Date : 2023-09-05 Meriem Fassatoui, Azadeh Saffarian, Céline Mulet, Henda Jamoussi, Amel Gamoudi, Yossra Ben Halima, Meriem Hechmi, Sonia Abdelhak, Abdelmajid Abid, Philippe J Sansonetti, Thierry Pedron, Rym Kefi
Gut microbiota plays a key role in the regulation of metabolism and immunity. We investigated the profile of gut microbiota and the impact of dietary intake on gut bacterial distribution in diabetic and healthy Tunisian subjects, aiming to identify a dysbiotic condition, hence opening the way to restore eubiosis and facilitate return to health. In the present research, we enrolled 10 type 1 diabetic
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Investigation of Altered Retinal Microvasculature in Female Patients with Rheumatoid Arthritis: Optical Coherence Tomography Angiography Detection. Biosci. Rep. (IF 4.0) Pub Date : 2023-09-04 Hsuan-Yi Lee, Jun Chen, Pin Ying, San-Hua Xu, Min Kang, Jie Zou, Xu-Lin Liao, Wenqing Shi, Qian Ling, Yi-Xin Wang, Hong Wei, Yi Shao
Rheumatoid arthritis (RA) is a chronic, systemic autoimmune disorder that primarily causes symmetrical polyarthritis and bone deformity. In RA patients, sight-threatening inflammatory eye complications would be expected. Objective: To ascertain the macular retinal vessel density changes in RA patients and controls using optical coherence tomography angiography (OCTA), and to investigate the association
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