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Clinicopathological Significance and Prognostic Role of High Mobility Group Box 1 (HMGB1), Toll-Like Receptor (TLR) 2 and TLR4 in Breast Cancer Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-04-17 Reina Taguchi, Mio Yamaguchi-Tanaka, Kiyoshi Takagi, Ai Sato, Yasuhiro Miki, Minoru Miyashita, Takashi Suzuki
High-mobility group box 1 (HMGB1) functions as damage-associated molecular pattern (DAMPs), released into extracellular space during cellular stress. Extracellular HMGB1 act as signal molecules through Toll-like receptor (TLR) 2 or TLR4, exerting diverse functions in both normal cells and malignant cells including breast cancer. However, their comprehensive examination in breast cancer tissues is lacking
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Pathophysiological Implications of Protein Lactylation in Pancreatic Epithelial Tumors Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-04-04 Tomoki Takata, Akihiro Nakamura, Hiroaki Yasuda, Hayato Miyake, Yoshio Sogame, Yuki Sawai, Michiyo Hayakawa, Kentaro Mochizuki, Ryuta Nakao, Takehiro Ogata, Hisashi Ikoma, Eiichi Konishi, Yoshinori Harada, Eigo Otsuji, Yoshito Itoh, Hideo Tanaka
Protein lactylation is a post-translational modification associated with glycolysis. Although recent evidence indicates that protein lactylation is involved in epigenetic gene regulation, its pathophysiological significance remains unclear, particularly in neoplasms. Herein, we investigated the potential involvement of protein lactylation in the molecular mechanisms underlying benign and malignant
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C-C Chemokine 21-Expressing T-cell Zone Fibroblastic Reticular Cells, Abundant in Lymph Nodes, Are Absent in Cancer Lymphoid Stroma Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-04-04 Haruo Ohtani, Kazuhiko Matsuo, Kosuke Kitahata, Eiichi Sato, Takashi Nakayama
Cancer tissue generally possesses an immunosuppressive microenvironment. However, some cancers are associated with lymphoid stroma (i.e., a widely developed tertiary lymphoid structure). The T-cell zone (paracortex) of secondary lymphoid organs, particularly lymph nodes, is characterized by an abundance of T-cell zone fibroblastic reticular cells (TCZ-FRCs) that express C-C motif chemokine ligand 21
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Membrane-Targeted palGFP Predominantly Localizes to the Plasma Membrane but not to Neurosecretory Vesicle Membranes in Rat Oxytocin Neurons Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-04-04 Hirotaka Sakamoto, Ayumu Inutsuka
Recent advances in viral vector technology, specifically using adeno-associated virus (AAV) vectors, have significantly expanded possibilities in neuronal tracing. We have utilized the Cre/loxP system in combination with AAV techniques in rats to explore the subcellular localization of palmitoylation signal-tagged GFP (palGFP) in oxytocin-producing neurosecretory neurons. A distinctive branching pattern
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The Mouse Model of Internal Capsule Demyelination: A Novel Tool for Investigating Motor Functional Changes Caused by Demyelination and for Evaluating Drugs That Promote Remyelination Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-02-29 Reiji Yamazaki, Nobuhiko Ohno
Multiple sclerosis (MS) is an inflammatory demyelinating disease of the central nervous system, characterized by remyelination failure and axonal dysfunction. Remyelination by oligodendrocytes is critical for improvement of neurological deficits associated with demyelination. Rodent models of demyelination are frequently used to develop and evaluate therapies for MS. However, a suitable mouse model
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The Effects of Corticosteroid Administration and Treadmill Exercise on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in Rats Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-02-29 Akinori Kaneguchi, Kaoru Yamaoka, Junya Ozawa
We aimed to investigate the effects of short-term corticosteroid administration after anterior cruciate ligament (ACL) reconstruction on marrow adipose tissue (MAT) and trabecular bone mass, as well as to examine whether treadmill exercise can mitigate MAT increase and trabecular bone deterioration caused by corticosteroid. ACL-reconstructed rats were divided into groups: no intervention, daily treadmill
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Glucose Induces ER Stress Response-Mediated Peritoneal Mesothelial Cell Death Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-02-29 Junichi Nakamata, Hiroyuki Morimoto, Ryoko Baba, Keiji Kokubu, Tetsu Miyamoto
Peritoneal dialysis (PD) fluid, which contains a high concentration of glucose, is involved in peritoneal damage after long-term use. The mechanisms through which glucose induces damage to the mesothelium have not been clearly elucidated. Although, endoplasmic reticulum (ER) stress response is associated with several diseases, the involvement of ER stress in peritoneal damage has not yet been demonstrated
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Effects of Weight Bearing on Marrow Adipose Tissue and Trabecular Bone after Anterior Cruciate Ligament Reconstruction in the Rat Proximal Tibial Epiphysis Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-02-29 Akinori Kaneguchi, Kaoru Yamaoka, Junya Ozawa
The effects of mechanical unloading after anterior cruciate ligament (ACL) reconstruction on bone and marrow adipose tissue (MAT) are unclear. We investigated weight bearing effects on bone and MAT after ACL reconstruction. Rats underwent unilateral knee ACL transection and reconstruction, followed by hindlimb unloading (non-weight bearing), no intervention (low-weight bearing, the hindlimb standing
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Aquaporin-5 Protein Is Selectively Reduced in Rat Parotid Glands under Conditions of Fasting or a Liquid Diet Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-02-29 Yoshie Fujita, Akie Taniguchi, Hanako Yamamoto, Hideru Obinata, Hiroshi Kogo, Akiko Iizuka-Kogo, Maiko Ikezawa, Yukiko Tajika, Satoshi Yokoo, Toshiyuki Matsuzaki
Aquaporin-5 (AQP5) water channel, transmembrane protein 16A (TMEM16A) Ca2+-activated Cl− channel, and Na+-K+-2Cl− cotransporter (NKCC1) are membrane proteins on salivary gland acinar cells that function in watery saliva secretion. We examined their expression changes in rat parotid glands under reduced mastication. Rats were either fed regular chow as a control group, fasted for 48 hr or fed a liquid
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Tissue-Targeted Transcriptomics Reveals SEMA3D Control of Hypoglossal Nerve Projection to Mouse Tongue Primordia Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2024-02-29 Taisuke Hani, Kazuya Fujita, Tomoo Kudo, Yuji Taya, Kaori Sato, Yuuichi Soeno
The mouse hypoglossal nerve originates in the occipital motor nuclei at embryonic day (E)10.5 and projects a long distance, reaching the vicinity of the tongue primordia, the lateral lingual swellings, at E11.5. However, the details of how the hypoglossal nerve correctly projects to the primordia are poorly understood. To investigate the molecular basis of hypoglossal nerve elongation, we used a novel
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Localization of Sites of Osimertinib Action in Rat Intestine, Skin, and Lung by Immunohistochemistry Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-12-28 Yuta Yamamoto, Tetsuya Saita, Hiroto Kataoka, Rintaro Sogawa, Sakiko Kimura, Shinya Kimura, Chisato Shimanoe, Masashi Shin
Osimertinib is a third-generation, irreversible tyrosine kinase inhibitor (TKI) of epidermal growth factor receptor (EGFR) that selectively inhibits both EGFR-TKI-sensitizing and EGFR T790M resistance mutations and has shown efficacy in patients with non-small-cell lung cancer. In this study, we created osimertinib-specific antibodies and developed an immunohistochemistry (IHC) for locating the sites
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High Concentrations of Nucleotides Prevent Capillary Regression during Hindlimb Unloading by Inhibiting Oxidative Stress and Enhancing Mitochondrial Metabolism of Soleus Muscles in Rats Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-12-28 Ryosuke Nakanishi, Nagisa Hashimoto, Miho Takuwa, Jihao Xing, Mikiko Uemura, Badur un Nisa, Masayuki Tanaka, Takumi Hirabayashi, Minoru Tanaka, Hidemi Fujino
Prolonged inactivity in skeletal muscles decreases muscle capillary development because of an imbalance between pro- and antiangiogenic signals, mitochondrial metabolism disorders, and increased oxidative stress. Nucleotides have been shown to exert a dose-dependent effect on disuse-induced muscle atrophy. However, the dose-dependent effect on capillary regression in disused muscles remains unclear
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Distribution of Contractile Structures in a Mouse and Human Lymph Node Associated with Lymph Flow Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-12-28 Yasuhisa Ishi, Takuya Omotehara, Xi Wu, Miyuki Kuramasu, Shinichi Kawata, Masahiro Itoh
Lymph nodes have contractile structures, but their distribution in a lymph node has been less considered in terms of facilitation of lymph flow. Axillary, inguinal, and mesenteric lymph nodes were collected from mice and human cadavers, and their sections were immunostained for alpha-smooth muscle actin (αSMA) and high molecular weight caldesmon (H-caldesmon). The αSMA-positive cells were localized
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Visualization of Amyloid Oligomers in the Brain of Patients with Alzheimer’s Disease Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-12-28 Ikuo Tooyama, Tomoko Kato, Hiroyasu Taguchi, Yusuke Kageyama, Kazuhiro Irie, Yukie Hirahara, Daijiro Yanagisawa
In the pathogenesis of Alzheimer’s disease (AD), highly neurotoxic amyloid-β (Aβ) oligomers appear early, they are thus considered to be deeply involved in the onset of Alzheimer’s disease. However, Aβ oligomer visualization is challenging in human tissues due to their multiple forms (e.g., low- and high-molecular-weight oligomers, including protofibrils) as well as their tendency to rapidly change
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Analysis of Insulinoma-Associated Protein 1 Expression in Pituitary Neuroendocrine Tumors Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-12-28 Yu Hirokawa, Chie Inomoto, Kenichi Oyama, Shigeyuki Tahara, Robert Y. Osamura, Takayuki Shiomi, Akira Matsuno
Insulinoma-associated protein 1 (INSM1) is a representative diagnostic marker of neuroendocrine neoplasms (NENs); however, it has not yet been used to diagnose pituitary neuroendocrine tumors (PitNETs), according to the 2022 World Health Organization (WHO) classification of pituitary tumors. This study aimed to examine the expression of INSM1 using immunohistochemistry, in the various cell lineages
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Cyclin D3 Colocalizes with Myogenin and p21 in Skeletal Muscle Satellite Cells during Early-Stage Functional Overload Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-12-28 Minenori Ishido
Myogenic cell differentiation is modulated by multiple regulatory factors, such as myogenin, p21, and cyclin D3 during myogenesis in vitro. It is also recognized that myogenin and p21 play important roles in regulating muscle satellite cell (SC) differentiation during overload-induced muscle hypertrophy in vivo. However, the expression patterns and functional role of cyclin D3 in the progress of muscle
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Immunohistochemical Analyses of Mammalian Target of Rapamycin (mTOR) Expression in Pituitary Neuroendocrine Tumors (PitNETs): mTOR as a Therapeutic Target for Functional PitNETs Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-12-28 Ichiro Nakazato, Takayuki Shiomi, Kenichi Oyama, Akira Matsuno, Chie Inomoto, R. Yoshiyuki Osamura
Current therapeutic modalities for pituitary neuroendocrine tumors (PitNETs) include medication, surgery, and radiotherapy. Some patients have tumors that are refractory to current modalities. Therefore, novel treatment options are needed for patients with intractable diseases. Consequently, we examined the pathological data of PitNETs to study medical therapies. We retrospectively studied 120 patients
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A Single Administration of Progesterone during the Neonatal Period Shows No Structural Changes in Male Reproductive Tracts in Mice Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-12-28 Takuya Omotehara, Hiroki Nakata, Kenta Nagahori, Miyuki Kuramasu, Koichiro Ichimura, Masahiro Itoh
The concentration of female-dominant steroid hormones, such as progesterone and estrogen, drops after birth in neonates. We have reported that neonatal estrogen treatment results in inflammation in the epididymis after puberty in male mice. Our recent study discovered that progesterone receptor was specifically expressed in efferent ducts just before birth in male mice. Therefore, this study aimed
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Beneficial Effect of Erythropoietin on Ameliorating Propionic Acid-Induced Autistic-Like Features in Young Rats Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-10-31 Sara A. Hosny, Alshaymaa M. Abdelmenem, Taha Azouz, Samaa S. Kamar, Asmaa M. ShamsEldeen, Asmaa A. El-Shafei
Autism is a neurodevelopmental disorder that impairs communication and social interaction. This study investigated the possible beneficial effects of erythropoietin (EPO) on experimental autistic-like behaviors induced by propionic acid (PPA). Twenty-four rats were distributed into three groups: (i) control; (ii) PPA_Gp: daily injected subcutaneously with PPA for five consecutive days; PPA+EPO-Gp:
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Immunolocalization of Cytoplasmic ER in ER-negative Breast Carcinoma as a Potent Favorable Prognostic Predictor Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-08-30 Akiko Ebata, Takashi Suzuki, Narumi Shoji-Harada, Yohei Hamanaka, Minoru Miyashita, Erina Iwabuchi, Kiyoshi Takagi, Yasuhiro Miki, Hiroshi Tada, Takanori Ishida
It is known that estrogen receptor (ER) has extranuclear signaling functions in addition to classical genomic pathway, and estrogenic actions have been reported in ER-negative breast carcinoma cells. However, significance of cytoplasmic-ER immunoreactivity has not been reported in ER-negative breast carcinoma tissues. We immunolocalized cytoplasmic ER in 155 ER-negative breast carcinoma tissues and
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Muscarinic Receptor Stimulation Does Not Inhibit Voltage-dependent Ca2+ Channels in Rat Adrenal Medullary Chromaffin Cells Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-08-30 Keita Harada, Masumi Inoue
Adrenal medullary chromaffin (AMC) and sympathetic ganglion cells are derived from the neural crest and show a similar developmental path. Thus, these two cell types have many common properties in membrane excitability and signaling. However, AMC cells function as endocrine cells while sympathetic ganglion cells are neurons. In rat sympathetic ganglion cells, muscarinic M1 and M4 receptors mediate
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Histone Modification in Histochemistry and Cytochemistry Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-06-27 Riko Kitazawa, Ryuma Haraguchi, Sohei Kitazawa
Keeping chromatin in a stable state is essential for genome stability, scheduled transcription, replication, DNA repair, and precise and reliable chromosome segregation and telomere maintenance during cell division. Over the past decade, research on chromatin remodeling has made great strides whereby modification of histone proteins is a key factor involved in many of the essential cellular processes
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Recent Advances in High-sensitivity In Situ Hybridization and Costs and Benefits to Consider When Employing These Methods Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-06-27 Shimpei Higo, Hirotaka Ishii, Hitoshi Ozawa
In situ hybridization (ISH), which visualizes nucleic acids in tissues and cells, is a powerful tool in histology and pathology. Over 50 years since its invention, multiple attempts have been made to increase the sensitivity and simplicity of these methods. Therefore, several highly sensitive in situ hybridization methods have been developed that offer researchers a wide range of options. When selecting
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SRY-box Transcription Factor 6 Is Expressed Not Only in the Dorsal but Also in the Ventral Zone of the Neural Tube and Is Highly Expressed in the Notochord and Chordoma Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-06-27 Genshu Tate
In the course of SRY-box transcription factor 6 (SOX6) expression profiling in human embryonic tissue, SOX 6 was found to be highly expressed in the notochord, based on the findings of immunohistochemistry (IHC). Sox6 is also expressed in the neural tube and the distribution of SOX6 is located in the ventral and dorsal zones of the neural tube. In contrast to the findings that SOX6-positive cells were
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Immunohistochemical Analysis of Mitochondrial Ferritin in the Midbrain of Patients with Parkinson’s Disease Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-04-25 Haruka Tsubaki, Daijiro Yanagisawa, Yusuke Kageyama, Zulzikry Hafiz Abu Baker, Ken-ichi Mukaisho, Ikuo Tooyama
Mitochondrial ferritin (FtMt) is an endogenous iron-storage protein localized in the mitochondria. FtMt is mainly observed in restricted tissues, such as those in the testis, islets of Langerhans, and brain. Further, it may protect cells from oxidative stress in neurodegenerative diseases, including Alzheimer’s disease and progressive supranuclear palsy. However, the role of FtMt in Parkinson’s disease
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Detection of SARS-CoV-2 and Its Related Factors on the Mucosal Epithelium of the Tongue Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-04-25 Jun Tamiya, Wakako Sakaguchi, Kimiko Nakagawa, Toshiharu Yamamoto, Juri Saruta, Nobuhisa Kubota, Akira Kawata, Iwao Hasegawa, Nobushiro Hamada, Keiichi Tsukinoki
SARS-CoV-2 infects a variety of tissues, including the oral cavity. However, there are few reports examining the association of SARS-CoV-2 with tongue mucosal tissues with sticky tongue debris. This study investigated the presence of SARS-CoV-2 and its associated molecules by dissecting tongue tissue from autopsy specimens of 23 patients who died of COVID-19-related illness (pneumonia). Immunohistochemical
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Application of Immunohistochemistry in Clinical Practices as a Standardized Assay for Breast Cancer Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-02-28 Shinobu Masuda, Yoko Nakanishi
Immunohistochemistry (IHC) has become an indispensable tool in the clinical practices for breast cancer; however, to achieve its standardization, numerous issues need to be overcome. In this review, we describe the development of IHC as an important clinical tool, and the challenges in standardizing IHC results for patients. We also present ideas for resolving the remaining issues and unmet needs,
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Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2023-02-28 Nevra Aydemir Celep, Semin Gedikli
This study, it was investigated whether silymarin has a protective effect by performing histological, immunohistochemical, and biochemical evaluations on the liver damage induced by cecal ligation perforation (CLP). CLP model was established and silymarin was treated at a dose of 50 mg/kg, 100 mg/kg, and 200 mg/kg, by oral one hour before the CLP. As an effect of the histological evaluations of the
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Low Doses of Bisphenol A Disrupt Neuronal Differentiation of Human Neuronal Stem/Progenitor Cells Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-12-28 Kaori Kiso-Farnè, Takeshi Yaoi, Takahiro Fujimoto, Kyoko Itoh
Bisphenol A (BPA) is an endocrine disrupting chemical. Human epidemiological studies have suggested that adverse neurobehavioral outcomes are induced by fetal exposure to BPA. The remarkable differences in the corticogenesis between human and agyrencephalic mammals are an increase in the intermediate progenitor cells (IPCs) and a following increase in the subplate thickness. It is uncertain whether
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Immunohistochemical Localization of Alogliptin, a DPP-4 Inhibitor, in Tissues of Normal and Type 2 Diabetes Model Rat Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-12-28 Yutaro Yamamoto, Kanae Ura, Takuma Matsukawa, Tetsuya Saita, Masashi Shin
We investigated the pharmacokinetics of alogliptin (AG) at the cell and tissue level in healthy Wistar rats and a type 2 diabetic Goto-Kakizaki (GK) rat model. Immunohistochemistry of the renal tissue in these rats, post 1 hr of AG administration, showed that the signal was observed in the glomeruli, proximal tubule S3 segments, distal tubules, collecting ducts, and only in the brush border of the
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Cathepsin D Inhibits Angiogenesis in Pituitary Neuroendocrine Tumors Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-12-28 Ren Fujiwara, Hirotomo Ten, Hui Chen, Chuan-lu Jiang, Ken-ichi Oyama, Keisuke Onoda, Akira Matsuno
Prolactin and growth hormone can acquire anti-angiogenic properties after undergoing proteolytic cleavage by Cathepsin D and bone morphogenetic protein 1 (BMP-1) into fragments known as vasoinhibins. Little is known about the effect of vasoinhibins on angiogenesis through the involvement of key cleavage enzymes Cathepsin D and BMP-1 in pituitary neuroendocrine tumors (PitNETs, formerly pituitary adenomas)
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Protective Effect of Ebselen on Ischemia-reperfusion Injury in Epigastric Skin Flaps in Rats Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-10-28 Takahiko Kuroki, Susumu Takekoshi, Kanae Kitatani, Chikara Kato, Muneo Miyasaka, Tadashi Akamatsu
The purpose of this study was to determine the role of oxidized diacylglycerol (DAG) and the molecular mechanism underlying ischemia-reperfusion (I/R) injury in rat skin flaps. The protective effect of ebselen on the viability of rat skin flaps with I/R injury was investigated. Flaps were designed and raised in the left inguinal region. Then, a microvascular clamp was applied to the vascular pedicle
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An Advanced Detection System for In Situ Hybridization Using a Fluorescence Resonance Energy Transfer-based Molecular Beacon Probe Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-10-28 Narantsog Choijookhuu, Yasuaki Shibata, Takumi Ishizuka, Yan Xu, Takehiko Koji, Yoshitaka Hishikawa
In situ hybridization (ISH) is a powerful method for detecting specific RNAs at the cellular level. Although conventional ISH using hapten-labeled probes are useful for detecting multiple RNAs, the detection procedures are still complex and required longer time. Therefore, we introduced a new application of fluorescence resonance energy transfer (FRET)-based molecular beacon (MB) probes for ISH. MCF-7
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Enzyme-labeled Antigen Method: Factors Influencing the Deterioration of Antigen-binding Activity of Specific Antibodies during Formalin Fixation and Paraffin Embedding Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-10-28 Yasuyoshi Mizutani, Kazuya Shiogama, Ken-ichi Inada, Toshiyuki Takeuchi, Atsuko Niimi, Motoshi Suzuki, Yutaka Tsutsumi
The enzyme-labeled antigen method is an immunohistochemical technique detecting plasma cells producing specific antibodies in tissue sections. The probe is an antigen labeled with an enzyme or biotin. This immunohistochemical technique is appliable to frozen sections of paraformaldehyde (PFA)-fixed tissues, but it has been difficult to apply it to formalin-fixed, paraffin-embedded (FFPE) sections.
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Optimized Mouse-on-mouse Immunohistochemical Detection of Mouse ESR2 Proteins with PPZ0506 Monoclonal Antibody Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-10-28 Mina Ozawa, Yujiro Hattori, Shimpei Higo, Mai Otsuka, Keisuke Matsumoto, Hitoshi Ozawa, Hirotaka Ishii
Despite the physiological significance of ESR2, a lack of well-validated detection systems for ESR2 proteins has hindered progress in ESR2 research. Thus, recent identification of a specific anti-human ESR2 monoclonal antibody (PPZ0506) and its specific cross-reactivity against mouse and rat ESR2 proteins heightened momenta toward development of appropriate immunohistochemical detection systems for
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Role of Cancer Stem-like Cells in the Process of Invasion and Mesenchymal Transformation by a Reconstituted Triple-negative Breast Cancer Cell Population Resistant to p53-induced Apoptosis Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-10-28 Sana Inoue, Miki Imanishi, Ai Kanzaki, Atsumi Fujimoto, Marina Maeyama, Ayaka Okamoto, Hiroka Matsuda, Kiyotsugu Yoshikawa, Rei Takahashi
We investigated the role of cancer stem cells (CSCs) in a population of triple-negative breast cancer (TNBC) cells that are resistant to apoptosis. A human breast cancer cell population capable of inducing p53 expression with doxycycline (Dox) was created and used as an untreated control (UT). After the addition of Dox to UT for 5 days, the cell population reconstituted with cells showing resistance
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Morphologic Analysis of M2 Macrophage in Glioblastoma: Involvement of Macrophage Extracellular Traps (METs) Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-08-27 Ayano Michiba, Kazuya Shiogama, Tetsuya Tsukamoto, Masaya Hirayama, Seiji Yamada, Masato Abe
Macrophages are classified into two phenotypes, M1 and M2, based on their roles. M2 macrophages suppress inflammation and increase in proportion to the malignancy of brain tumors. Recently, macrophage extracellular traps (METs), which change into a network, have been reported as a unique form of macrophage cell death. In this study, immunohistochemical analysis of macrophages in METs in human glioblastoma
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Pulmonary Neuroendocrine Cells and Small Cell Lung Carcinoma: Immunohistochemical Study Focusing on Mechanisms of Neuroendocrine Differentiation Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-06-29 Takaaki Ito, Shinji Kudoh, Kosuke Fujino, Mune Sanada, Yuki Tenjin, Haruki Saito, Yuko Nakaishi-Fukuchi, Hiroki Kameyama, Takaya Ichimura, Naoko Udaka, Noritaka Kudo, Akira Matsuo, Younosuke Sato
Neuroendocrine (NE) differentiation has been histochemically detected in normal and cancer tissues and cells. Immunohistochemical analyses have provided a more detailed understanding of NE biology and pathology. Pulmonary NE cells are a rare lung epithelial type, and small cell carcinoma of the lung (SCLC) is a high-grade NE tumor. Pulmonary NE and SCLC cells share common mechanisms for NE differentiation
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Current Status of Whole Slide Image (WSI) Standardization in Japan Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-06-29 Ichiro Mori
A whole slide image (WSI) is a digitized microscopic image that is particularly useful in histochemistry and cytochemistry. Several WSI scanners have been introduced in Japan and all use their own native format. Thus, there is basically no interchangeability. However, the Digital Imaging and Communications in Medicine (DICOM) standard format for WSI has been available since 2010. In this review, the
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A Histochemical Analysis of Neurofibrillary Tangles in Olfactory Epithelium, a Study Based on an Autopsy Case of Juvenile Alzheimer’s Disease Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-06-29 Shino Shimizu, Ichiro Tojima, Keigo Nakamura, Hideaki Kouzaki, Takeshi Kanesaka, Norihiro Ogawa, Yoshio Hashizume, Hiroyasu Akatsu, Akira Hori, Ikuo Tooyama, Takeshi Shimizu
The pathological changes of Alzheimer’s disease (AD) begin 10–20 years before clinical onset, and it is therefore desirable to identify effective methods for early diagnosis. The nasal mucosa is a target tissue for measuring AD-related biomarkers because the olfactory nerve is the only cranial nerve that is exposed to the external environment. We describe an autopsy case of rapidly advanced juvenile
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EPLINβ Is Involved in the Assembly of Cadherin-catenin Complexes in Osteoblasts and Affects Bone Formation Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-06-29 Shihoko Miyazaki, Taro Funamoto, Tomohisa Sekimoto, Syuji Kurogi, Tomomi Ohta, Takuya Nagai, Takuya Tajima, Mai Imasaka, Kumiko Yoshinobu, Kimi Araki, Masatake Araki, Narantsog Choijookhuu, Yoshitaka Hishikawa, Etsuo Chosa
Epithelial protein lost in neoplasm (EPLIN) is an actin-associated cytoskeletal protein that plays an important role in epithelial cell adhesion. EPLIN has two isoforms: EPLINα and EPLINβ. In this study, we investigated the role of EPLINβ in osteoblasts using EPLINβ-deficient (EPLINβGT/GT) mice. The skeletal phenotype of EPLINβGT/GT mice is indistinguishable from the wildtype (WT), but bone properties
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Label-free Assessment of the Nascent State of Rat Non-alcoholic Fatty Liver Disease Using Spontaneous Raman Microscopy Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-04-27 Masashi Takemura, Kentaro Mochizuki, Yoshinori Harada, Akira Okajima, Michiyo Hayakawa, Ping Dai, Yoshito Itoh, Hideo Tanaka
Spontaneous Raman microscopy, which can detect molecular vibrations in cells and tissues, could be a useful tool for the label-free assessment of non-alcoholic fatty liver disease (NAFLD). However, it is unclear whether it can be used to evaluate the nascent state of NAFLD. To address this, we analyzed the Raman spectra of rat liver tissues in the nascent state of NAFLD upon excitation at 532 nm. Raman
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Colonic Lymphoid Follicle Hyperplasia after Gastrectomy in Rats Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-04-27 Tomoko Kagiya, Kazuya Shiogama, Ken-Ichi Inada, Hirotoshi Utsunomiya, Masayuki Kitano
Nodular lymphoid hyperplasia (NLH) of the human colon has been associated with multiple diseases and symptoms. Causes include food allergies, infections, inflammatory bowel disease, and immunodeficiency, and gastrectomy is not usually considered to be the etiology. Nine rats two weeks after total gastrectomy and 12 control rats were sacrificed and submitted for histological examination. In the gastrectomy
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Specific Expression of KCC2 in the α Cells of Normal and Type 1 Diabetes Model Mouse Pancreatic Islets Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-02-26 Chigusa Shimizu-Okabe, Shigeki Okada, Shiki Okamoto, Hiroaki Masuzaki, Chitoshi Takayama
Gamma-aminobutyric acid (GABA) is an inhibitory neurotransmitter in the mature brain; however, it acts excitatory during development. This difference in action depends on the intracellular chloride ion concentration, primarily regulated by potassium chloride co-transporter2 (KCC2). Sufficient KCC2 expression results in its inhibitory action. GABA is also abundant in pancreatic islets, where it acts
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Identification of Brain Regions Activated by Sevoflurane and Propofol and Regional Changes in Gene Expression Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-02-26 Nobutaka Kamei, Shimpei Higo, Tomoki Mizuno, Keisuke Mori, Atsuhiro Sakamoto, Hitoshi Ozawa
General anesthetics have different efficacies and side effect incidences based on their mechanism of action. However, detailed comparative studies of anesthetics are incomplete. In this study, target brain regions and gene expression changes in these brain regions were determined for sevoflurane and propofol to understand the mechanisms that cause differences among anesthetics. Rats were anesthetized
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Immunolocalization of CD80 and CD86 in Non-Small Cell Lung Carcinoma: CD80 as a Potent Prognostic Factor Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-02-26 Takashi Sato, Kiyoshi Takagi, Mitsunori Higuchi, Hiroko Abe, Michie Kojimahara, Miho Sagawa, Megumi Tanaki, Yasuhiro Miki, Takashi Suzuki, Hiroshi Hojo
It has been demonstrated that tumor cells express programed cell death protein 1 (PD-L1) to escape T lymphocytes that express programed cell protein 1 (PD-1), and PD-1/PD-L1 immune checkpoint inhibitors have been regarded in lung cancer patients. CD80 and CD86 are members of B7 superfamily which regulates T lymphocyte activation and tolerance. However, immunolocalization of CD80 and CD86 has not been
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Histochemistry, Cytochemistry and Epigenetics Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-02-26 Sohei Kitazawa, Teruyuki Ohno, Ryuma Haraguchi, Riko Kitazawa
Over the past few decades, many researchers have individually identified tumor-related genes, and have accumulated information on their basic research in a database. With the development of technology that can comprehensively test the expression status within a short time, oncogene panel testing has become attainable. On the other hand, changes in gene expression that do not depend on changes in base
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Histomorphological Changes in a Rat Model of Polycystic Ovary Syndrome and the Contribution of Stevia Leaf Extract in Modulating the Ovarian Fibrosis, VEGF, and TGF-β Immunoexpressions: Comparison with Metformin Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2022-02-26 Ahmed A. Morsi, Ezat A. Mersal, Abdel Razik H. Farrag, Ahmed M. Abdelmoneim, Alshaymaa M. Abdelmenem, Mohamed S. Salim
Polycystic ovary syndrome (PCOS) is a common endocrine disorder of fertile females. It has been reported that stevia leaf extract (SLE) has antidiabetic and antihyperlipidemic properties. Therefore, the current study hypothesized and investigated the role and mechanistic aspects of a natural sweetener; SLE in treating a rat model of letrozole-induced PCOS and to compare it with metformin. Thirty-five
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The Visualization of Protein-Protein Interactions in Breast Cancer: Deployment Study in Pathological Examination Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-12-24 Erina Iwabuchi, Yasuhiro Miki, Hironobu Sasano
The therapeutic strategy is determined by protein expression using immunohistochemistry of estrogen receptor (ER), progesterone receptor, and human epidermal growth factor receptor 2 (HER2) in formalin-fixed paraffin-embedded (FFPE) breast cancer tissues. However, few proteins function independently, and many of them functions due to protein-protein interactions (PPIs) with other proteins. Therefore
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Differences in Regression Patterns of Complete and Incomplete Intestinal Metaplasia at Ten Years after Helicobacter pylori Eradication Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-12-24 Yasuhiro Wada, Masaaki Kodama, Kazuhiro Mizukami, Tadayoshi Okimoto, Takafumi Fuchino, Koshiro Tsutsumi, Masahide Fukuda, Yuka Hirashita, Kensuke Fukuda, Kazuhisa Okamoto, Ryo Ogawa, Ryoji Kushima, Kazunari Murakami
This study was conducted to reveal the reversibility of subtype of intestinal metaplasia (IM) and Paneth cells after H. pylori eradication (HPE). Among 75 patients, we retrospectively examined the proportions of patients with complete type of IM (CIM), incomplete type of IM (IIM) and Paneth cells in their biopsy specimens obtained from the greater curvature of the antrum (A2) and the greater curvature
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Nuclear Expression of Pygo2 Correlates with Poorly Differentiated State Involving c-Myc, PCNA and Bcl9 in Myanmar Hepatocellular Carcinoma Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-12-24 Myo Win Htun, Yasuaki Shibata, Kyaw Soe, Takehiko Koji
In Myanmar, hepatocellular carcinoma (HCC) is commonly seen in young adult and associated with poor prognosis, while the molecular mechanisms that characterize HCC in Myanmar are unknown. As co-activation of Wnt/β-catenin signaling and c-Myc (Myc) are reported to associate with malignancy of HCC, we immunohistochemically investigated the expression of Pygo2 and Bcl9, the co-activators of the Wnt/β-catenin
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Cold-induced Conversion of Connective Tissue Skeleton in Brown Adipose Tissues Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-10-29 Masako Yudasaka, Yuko Okamatsu-Ogura, Takeshi Tanaka, Kumiko Saeki, Hiromichi Kataura
Thermogenesis via fatty acid-induced uncoupled mitochondrial respiration is the primary function of brown adipose tissue (BAT). In response to changes in ambient temperatures, the weight and specific gravity of BAT change, depending on the quantity of lipid droplets stored in brown adipocytes (BA). Such conditions should result in the reconstruction of connective tissue skeletons, especially of collagen
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ZMYM3 May Promote Cell Proliferation in Small Cell Lung Carcinoma Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-10-29 Noritaka Kudo, Shinji Kudoh, Akira Matsuo, Yamato Motooka, Takaaki Ito
Zinc finger, myeloproliferative, and mental retardation-type containing 3 (ZMYM3) is a highly conserved protein among vertebrates. Although it promotes DNA repair and moderate histone acetylation, the other functions of ZMYM3 remain unclear. We herein examined the physiological functions of ZMYM3 in human lung cancer using a ZMYM3-knockdown small cell lung cancer (SCLC) cell line. ZMYM3-knockdown SCLC
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Tissue-specific Physical and Biological Microenvironments Modulate the Behavior of Cervical Squamous Cell Carcinoma Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-10-29 Katsuyuki Hanashima, Takashi Akutagawa, Mihoko Yamamoto-Rikitake, Takehisa Sakumoto, Maki Futamata, Yoshifumi Nakao, Masatoshi Yokoyama, Shuji Toda, Shigehisa Aoki
The mechanisms controlling the aggressiveness and survival of cervical SCC cells remain unclear. We investigated how the physical and biological microenvironments regulate the growth, apoptosis and invasiveness of cervical cancer cells. Dynamic flow and air exposure were evaluated as physical microenvironmental factors, and stromal fibroblasts were evaluated as a biological microenvironmental factor
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Effects of Endurance Exercise on Basement Membrane in the Soleus Muscle of Aged Rats Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-10-29 Yuji Kanazawa, Mamoru Nagano, Satoshi Koinuma, Shinichi Sugiyo, Yasufumi Shigeyoshi
The basement membrane (BM)-related factors, including collagen IV, are important for the maintenance and recovery of skeletal muscles. Aging impairs the expression of BM-related factors during recovery after disuse atrophy. Muscle activity facilitates collagen synthesis that constitutes the BM. However, the effect of endurance exercise on the BM of aged muscles is unclear. Thus, to understand the effect
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Dexmedetomidine and Phosphocreatine Post-treatment Provides Protection against Focal Cerebral Ischemia-reperfusion Injury in Rats Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-08-25 Xiaofen Sun, Fang Kang, Yuxian Shen, Yujun Shen, Juan Li
In this study we investigated the neuroprotective efficacy of dexmedetomidine (Dex) and phosphocreatine (PCr) alone or in combination in a rat model of focal cerebral ischemia-reperfusion injury (I/R). I/R was induced by intraluminal middle cerebral artery occlusion (MCAO) and reperfusion. Male Sprague-Dawley rats were randomly allocated to the Sham group and I/R group, and the I/R group was further
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Decreased Gastric Gland Mucin-specific O-glycans Are Involved in the Progression of Ovarian Primary Mucinous Tumours Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-08-25 Ayumi Ohya, Hisanori Matoba, Yasunari Fujinaga, Jun Nakayama
Ovarian primary mucinous tumours (OPMTs) show an adenoma–borderline–carcinoma sequence with gastrointestinal metaplasia. Gastric gland mucin-specific O-glycans are unique with an α1,4-linked N-acetylglucosamine (αGlcNAc) residue attached to mucin 6 (MUC6). Although αGlcNAc is expected to be expressed in OPMTs, the relationship between αGlcNAc expression and OPMT progression remains unknown. Here, we
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Forkhead Box I1 in Breast Carcinoma as a Potent Prognostic Factor Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-08-25 Yoshiaki Onodera, Kiyoshi Takagi, Yoshimi Neoi, Ai Sato, Mio Yamaguchi, Yasuhiro Miki, Akiko Ebata, Minoru Miyashita, Hironobu Sasano, Takashi Suzuki
Forkhead box (FOX) proteins are family of transcriptional factors and regulate cell growth and differentiation as well as embryogenesis and longevity. Previous studies have demonstrated that several FOX members regulate growth or metastasis of breast carcinoma, but clinical significance of total FOX members remains unclear. We first examined associations between expression of 40 FOX genes and TNM status
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A Review of miR-326 and Female Related Diseases Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-06-29 Li-na Lin, Qing-mei Zhang, Ying-ying Ge, Bin Luo, Xiao-xun Xie
MicroRNA (miRNA), a non-coding single-stranded RNA molecule with 20–23 nucleotides encoded by endogenous genes, plays an essential role in maintaining normal cell function and regulating cell proliferation, differentiation, apoptosis, autophagy, and cell metabolism. The imbalance between miRNA and genes can cause a series of diseases, including malignancies. miRNA-326 (miR-326) is extensively known
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Metabolic Reprogramming Drives Pituitary Tumor Growth through Epigenetic Regulation of TERT Acta Histochem. Cytochem. (IF 2.4) Pub Date : 2021-06-29 Hiromi Onizuka, Kenta Masui, Kosaku Amano, Takakazu Kawamata, Tomoko Yamamoto, Yoji Nagashima, Noriyuki Shibata
Pituitary adenomas are common, benign brain tumors. Some tumors show aggressive phenotypes including early recurrence, local invasion and distant metastasis, but the underlying mechanism to drive the progression of pituitary tumors has remained to be clarified. Aerobic glycolysis known as the Warburg effect is one of the emerging hallmarks of cancer, which has an impact on the tumor biology partly