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Fructose-1,6-bisphosphatase 1 dephosphorylates and inhibits TERT for tumor suppression Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-27 Min Li, Zheng Wang, Jingjing Tao, Hongfei Jiang, Huang Yang, Dong Guo, Hong Zhao, Xuxiao He, Shudi Luo, Xiaoming Jiang, Li Yuan, Liwei Xiao, Haiyan He, Rilei Yu, Jing Fang, Tingbo Liang, Zhengwei Mao, Daqian Xu, Zhimin Lu
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The death gaze of MEDUSA Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-26 Jesse D. Gelles, Jerry Edward Chipuk
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Reeling in microorganisms Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-25 Gene Chong
The metabolic engineering of microorganisms is an attractive platform for bioremediation and the industrial production of chemicals. However, biocontainment strategies are needed if genetically modified organisms are to be released into open environments. Hoffmann and Cai report a biocontainment strategy in yeast based on targeted protein degradation. The authors performed high-throughput screening
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Profiling the proximal proteome of the activated μ-opioid receptor Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-25 Benjamin J. Polacco, Braden T. Lobingier, Emily E. Blythe, Nohely Abreu, Prachi Khare, Matthew K. Howard, Alberto J. Gonzalez-Hernandez, Jiewei Xu, Qiongyu Li, Brandon Novy, Zun Zar Chi Naing, Brian K. Shoichet, Willow Coyote-Maestas, Joshua Levitz, Nevan J. Krogan, Mark Von Zastrow, Ruth Hüttenhain
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Targeting the chromatin binding of exportin-1 disrupts NFAT and T cell activation Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-25 Yi Fan Chen, Maryam Ghazala, Ryan M. Friedrich, Brittany A. Cordova, Frederick N. Petroze, Ramya Srinivasan, Kevin C. Allan, David F. Yan, Joel L. Sax, Kelley Carr, Suzanne L. Tomchuck, Yuriy Fedorov, Alex Y. Huang, Amar B. Desai, Drew J. Adams
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Stress it up Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-25 Grant Miura
AKT is a serine/threonine kinase that is essential for cellular metabolism and proliferation and is overactive in cancer cells. Small molecule catalytic inhibitors of all three AKT isoforms, as well as selective AKT degraders, have been developed, but the therapeutic efficacy of these compounds remains less than optimal. In particular, existing AKT degraders such as INY-03-041 elicit slow degradation
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Switching off the stress response Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-25 Yiyun Song
Cells respond to adverse environmental changes by activating stress response pathways. However, the continuous activation of stress responses can lead to unwanted cell death or damage to necessary biological functions, and it is not clear how stress response pathways are turned off once the condition improves. Haakonsen et al. have now reported the discovery of an E3 complex that switches off the stress
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De novo and somatic structural variant discovery with SVision-pro Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-22 Songbo Wang, Jiadong Lin, Peng Jia, Tun Xu, Xiujuan Li, Yuezhuangnan Liu, Dan Xu, Stephen J. Bush, Deyu Meng, Kai Ye
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Branched chemically modified poly(A) tails enhance the translation capacity of mRNA Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-22 Hongyu Chen, Dangliang Liu, Jianting Guo, Abhishek Aditham, Yiming Zhou, Jiakun Tian, Shuchen Luo, Jingyi Ren, Alvin Hsu, Jiahao Huang, Franklin Kostas, Mingrui Wu, David R. Liu, Xiao Wang
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Global signal peptide profiling reveals principles of selective Sec61 inhibition Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-22 Nicole A. Wenzell, Brian B. Tuch, Dustin L. McMinn, Matthew J. Lyons, Christopher J. Kirk, Jack Taunton
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Sulfide oxidation promotes hypoxic angiogenesis and neovascularization Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-20 Roshan Kumar, Victor Vitvitsky, Apichaya Sethaudom, Rashi Singhal, Sumeet Solanki, Sydney Alibeckoff, Harrison L. Hiraki, Hannah N. Bell, Anthony Andren, Brendon M. Baker, Costas A. Lyssiotis, Yatrik M. Shah, Ruma Banerjee
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Ripping and stitching with copper Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-21 Ninian J. Blackburn
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Ubiquitin-specific proximity labeling for the identification of E3 ligase substrates Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-21 Hai-Tsang Huang, Ryan J. Lumpkin, Ryan W. Tsai, Shuyao Su, Xu Zhao, Yuan Xiong, James Chen, Nada Mageed, Katherine A. Donovan, Eric S. Fischer, William R. Sellers
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Starfysh integrates spatial transcriptomic and histologic data to reveal heterogeneous tumor–immune hubs Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-21 Siyu He, Yinuo Jin, Achille Nazaret, Lingting Shi, Xueer Chen, Sham Rampersaud, Bahawar S. Dhillon, Izabella Valdez, Lauren E. Friend, Joy Linyue Fan, Cameron Y. Park, Rachel L. Mintz, Yeh-Hsing Lao, David Carrera, Kaylee W. Fang, Kaleem Mehdi, Madeline Rohde, José L. McFaline-Figueroa, David Blei, Kam W. Leong, Alexander Y. Rudensky, George Plitas, Elham Azizi
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Chemical proteomics to study metabolism, a reductionist approach applied at the systems level Cell Chem. Bio. (IF 8.6) Pub Date : 2024-03-21 Md Yousuf Ali, Liron Bar-Peled
Cellular metabolism encompasses a complex array of interconnected biochemical pathways that have historically relied on reductionist or bulk measurement approaches. Ali and Bar-Peled provide a primer on the power of chemical proteomic technologies for dissecting metabolism at scale and how this has enabled leaps in drug discovery.
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Identification of clickable HIV-1 capsid-targeting probes for viral replication inhibition Cell Chem. Bio. (IF 8.6) Pub Date : 2024-03-21 William M. McFadden, Mary C. Casey-Moore, Grant A.L. Bare, Karen A. Kirby, Xin Wen, Gencheng Li, Hua Wang, Ryan L. Slack, Alexa A. Snyder, Zachary C. Lorson, Isabella L. Kaufman, Maria E. Cilento, Philip R. Tedbury, Milan Gembicky, Arthur J. Olson, Bruce E. Torbett, K. Barry Sharpless, Stefan G. Sarafianos
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Bioorthogonal chemistry for biological insights Cell Chem. Bio. (IF 8.6) Pub Date : 2024-03-21 The Cell Chemical Biology editorial team
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Voices: Challenges and opportunities for bioorthogonal chemistry Cell Chem. Bio. (IF 8.6) Pub Date : 2024-03-21 Ku-Lung Hsu, Ben Schumann, Ellen Sletten, Ekaterina Vinogradova, Peng Zou
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Covalent hitchhikers guide proteins to the nucleus Cell Chem. Bio. (IF 8.6) Pub Date : 2024-03-21 Alexander F. Russell, Madeline F. Currie, Champak Chatterjee
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Combating the myth of “innate genius” in science Cell Chem. Bio. (IF 8.6) Pub Date : 2024-03-21 Sigrid Nachtergaele
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FLEX: genetically encodable enzymatic fluorescence signal amplification using engineered peroxidase Cell Chem. Bio. (IF 8.6) Pub Date : 2024-03-20 Nirmali Sharma, Minkyo Jung, Pratyush Kumar Mishra, Ji Young Mun, Hyun-Woo Rhee
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Design of amyloidogenic peptide traps Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-19 Danny D. Sahtoe, Ewa A. Andrzejewska, Hannah L. Han, Enrico Rennella, Matthias M. Schneider, Georg Meisl, Maggie Ahlrichs, Justin Decarreau, Hannah Nguyen, Alex Kang, Paul Levine, Mila Lamb, Xinting Li, Asim K. Bera, Lewis E. Kay, Tuomas P. J. Knowles, David Baker
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Making genome editing a success story in Africa Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-19 Hussein M. Abkallo, Patrick Arbuthnot, Thomas O. Auer, Dave K. Berger, Johan Burger, Ereck Chakauya, Jean-Paul Concordet, Abdoulaye Diabate, Vincenzo Di Donato, Jan-Hendrik Groenewald, Amadou Guindo, Lizette L. Koekemoer, Florence Nazare, Tony Nolan, Fredros Okumu, Emma Orefuwa, Lily Paemka, Lucia Prieto-Godino, Steven Runo, Marie Sadler, Kassahun Tesfaye, Leena Tripathi, Charles Wondji
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A retrotransposon for site-specific gene transfer Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-18 Fred Dyda, Alison B. Hickman
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World’s first TIL therapy approved Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15
Iovance Biotherapeutics has received a long-awaited go-ahead from the US Food and Drug Administration for its tumor-infiltrating lymphocyte (TIL) cancer therapy. The approval for Amtagvi (lifileucel) for treating patients with unresectable or metastatic melanoma is a milestone: it marks the first immune cell therapy approved for solid tumors, and it is also the first made from TILs. Amtagvi is a living
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The deubiquitinase function of ataxin-3 and its role in the pathogenesis of Machado-Joseph disease and other diseases Biochem. J. (IF 4.1) Pub Date : 2024-03-20 Potapenko, Anastasiya, Davidson, Jennilee M., Lee, Albert, Laird, Angela S.
Machado-Joseph disease (MJD) is a devastating and incurable neurodegenerative disease characterised by progressive ataxia, difficulty speaking and swallowing. Consequently, affected individuals ultimately become wheelchair dependent, require constant care, and face a shortened life expectancy. The monogenic cause of MJD is expansion of a trinucleotide (CAG) repeat region within the ATXN3 gene, which
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Chemical immunology: Recent advances in tool development and applications Cell Chem. Bio. (IF 8.6) Pub Date : 2024-03-18 Yujie Shi, Eleanor E. Bashian, Yingqin Hou, Peng Wu
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Structural analysis of a U-superfamily conotoxin containing a mini-granulin fold: Insights into key features that distinguish between the ICK and granulin folds J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-18 Tiziano Raffaelli, David T. Wilson, Sebastien Dutertre, Julien Giribaldi, Irina Vetter, Samuel D. Robinson, Ashvriya Thapa, Antin Widi, Alex Loukas, Norelle L. Daly
We are entering an exciting time in structural biology where artificial intelligence can be used to predict protein structures with greater accuracy than ever before. Extending this level of accuracy to the predictions of disulfide-rich peptide structures is likely to be more challenging, at least in the short term, given the tight packing of cysteine residues and the numerous ways that the disulfide
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YAP/TAZ-mediated regulation of laminin 332 is enabled by β4 integrin repression of ZEB1 to promote ferroptosis resistance J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-18 Hira Lal Goel, Emmet R. Karner, Ayush Kumar, Dimpi Mukhopadhyay, Shivam Goel, Arthur M. Mercurio
We are interested in the contribution of integrins and the extracellular matrix to epithelial differentiation in carcinomas. This study was motivated by our finding that the Hippo effectors YAP and TAZ can sustain the expression of laminin 332 (LM332), the predominant ECM ligand for the integrin β4, in breast carcinoma cells with epithelial differentiation. More specifically, we observed that YAP and
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The structures of salt inducible kinase 3 in complex with pharmacological inhibitors reveal determinants for binding and selectivity J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-18 Linda Öster, Marie Castaldo, Emma de Vries, Fredrik Edfeldt, Nils Pemberton, Euan Gordon, Linda Cederblad, Helena Käck
The salt-inducible kinases (SIKs) 1-3, belonging to the AMPK-related kinase family, serve as master regulators orchestrating a diverse set of physiological processes such as metabolism, bone formation, immune response, oncogenesis and cardiac rhythm. Owing to its key regulatory role, the SIK kinases have emerged as compelling targets for pharmacological intervention across a diverse set of indications
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Non-transcriptional IRF7 interacts with NF-κB to inhibit viral inflammation J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-18 Shumin Fan, Sonam Popli, Sukanya Chakravarty, Ritu Chakravarti, Saurabh Chattopadhyay
Interferon (IFN) regulatory factors (IRF) are key transcription factors in cellular antiviral responses. IRF7, a virus-inducible IRF, expressed primarily in myeloid cells, is required for transcriptional induction of IFNα and antiviral genes. IRF7 is activated by virus-induced phosphorylation in the cytoplasm, leading to its translocation to the nucleus for transcriptional activity. Here, we revealed
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Highly Pathogenic PRRSV Upregulates IL-13 Production through Nonstructural Protein 9 mediated Inhibition of N6-Methyladenosine(m6A) Demethylase FTO J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-18 Xingyu Gong, Yuan Liang, Jingjing Wang, Yipeng Pang, Xiaohan Chen, Qiaoya Zhang, Chengchuang Song, Yanhong Wang, Chunlei Zhang, Xingtang Fang, Xi Chen
Porcine reproductive and respiratory syndrome virus (PRRSV), a highly infectious and causes severe losses in the swine industry by regulating the inflammatory response, inducing tissue damage, suppressing the innate immune response, and promoting persistent infection in hosts. Interleukin-13 (IL-13) is a cytokine that plays a critical role in regulating immune responses and inflammation, particularly
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The SCF-FBW7β E3 ligase mediates ubiquitination and degradation of the serine/threonine protein kinase PINK1 J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-18 Seo Jeong Jeon, Kwang Chul Chung
Understanding the mechanisms that govern the stability of functionally crucial proteins is essential for various cellular processes, development, and overall cell viability. Disturbances in protein homeostasis are linked to the pathogenesis of neurodegenerative diseases (NDDs). PINK1, a protein kinase, plays a significant role in mitochondrial quality control and cellular stress response, and its mutated
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Mechanism of GTPase activation of a prokaryotic small Ras-like GTPase MglA by an asymmetrically interacting MglB dimer J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-18 Sukanya Chakraborty, Manil Kanade, Pananghat Gayathri
Cell polarity oscillations in motility are driven by a prokaryotic small Ras-like GTPase, MglA, which switches from one cell pole to the other in response to extracellular signals. MglA dynamics is regulated by MglB, which functions both as a GAP (GTPase activating protein) and a GEF (guanine nucleotide exchange factor) for MglA. With an aim to dissect the asymmetric role of the two MglB protomers
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Alternative autophagy dampens UVB-induced NLRP3 inflammasome activation in human keratinocytes J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-17 Tatsuya Hasegawa, Saori Noguchi, Masaya Nakashima, Masashi Miyai, Makiko Goto, Yuko Matsumoto, Satoru Torii, Shinya Honda, Shigeomi Shimizu
Sunlight exposure results in an inflammatory reaction of the skin commonly known as sunburn, which increases skin cancer risk. In particular, the ultraviolet B (UVB) component of sunlight induces inflammasome activation in keratinocytes to instigate the cutaneous inflammatory responses. Here, we explore the intracellular machinery that maintains skin homeostasis by suppressing UVB-induced inflammasome
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Supercharging T cell therapy with cancer mutations Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15 Iris Marchal
T cell therapies are still hindered by poor T cell persistence and function, making them largely ineffective against solid tumors. Human cancerous T cells acquire mutations that increase their fitness and evade immune challenges in similar situations to those faced by therapeutic T cells. Writing in Nature, Garcia and colleagues exploit the fitness-enhancing abilities of these cancer mutations by incorporating
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US bill targets Chinese biotechs Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15
The United States is seeking to prevent four Chinese biotech companies from doing business in the country, citing them as “companies of concern” that threaten national security. The Biosecure Act, introduced in both the Senate (S.3558) in December and House of Representatives (H.R.7085) in January, would prohibit the federal government from contracting with certain biotech providers connected to foreign
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Wearable technology and devices Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15
Recent patents relating to wearable technology and devices for patient monitoring and treatment of health conditions.
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A synthetic antibiotic overcomes antimicrobial resistance Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15 Iris Marchal
Many small-molecule antibiotics function by disrupting bacterial ribosomes, but bacteria develop resistance by modifying ribosomes to reduce the binding affinity of these molecules. Writing in Science, Wu et al. present a solution to this challenge by engineering a synthetic antibiotic that remains locked in an optimal conformation that boosts ribosomal binding. The authors designed the molecule cresomycin
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In vivo CRISPR agent cuts HAE attacks 95% Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15
A CRISPR–Cas9-based gene editing therapy from Intellia Therapeutics reduced monthly swelling attacks by 95% in people with hereditary angioedema (HAE). The results from a small phase 1 trial were published in the New England Journal of Medicine in February. HAE is a rare genetic disorder characterized by recurrent bouts of subcutaneous and submucosal swelling that can be life threatening. Kallikrein
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Predicting the structure of large protein complexes Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15 Iris Marchal
Deep learning models like RoseTTAFold and AlphaFold2 allow highly accurate protein structure prediction, but large protein assemblies remain hard to predict because of their size and complex subunit interactions. In a study published in Nature Methods, Shor and Scheidman-Duhovny introduce CombFold, a combinatorial and hierarchical assembly algorithm that predicts structures of large protein complexes
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Cell-based coffee future-proofs world’s favorite brew Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15
Lior Raviv, Pluri’s chief technical officer, says: “We hypothesized we could take the cells from the plant and put them in a bioreactor [to grow coffee].” Through their work in cell therapy and cultivated meat, the Pluri team knew that not all cells like the same growing conditions. Taking plant cell samples, they made cell lines and, instead of growing them swirling around in suspension culture, they
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Biotech news from around the world Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15
Saudi Arabia announces a plan to become a biotech leader in the Middle East and North Africa region by 2030 and an international biotech hub by 2040. Its National Biotechnology Strategy aims to grow the country’s capabilities in vaccines, biomanufacturing, genomics and plant optimization to increase job creation and drive economic growth and diversification. The Ministry of Health and Welfare invests
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People Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15
Recent moves of note in and around the biotech and pharma industries.
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2H23 biotech job picture Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15 Michael Francisco
A semiannual snapshot of job expansions, reductions and availability in the biotech and pharma sectors.
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Lighting the match: how a student-led career mentorship program can blaze the way for non-academic careers Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15 Anna Bagnell, Alana MacDonald, Peter Espenshade, Mark Schenerman
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Biologic patent challenges under the America Invents Act Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15 Victor L. Van de Wiele, Aaron S. Kesselheim, S. Sean Tu
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Innovators want pills to treat sickle cell disease. Can they match gene therapy? Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-15
Although CRISPR-based gene therapy for sickle cell disease offers transformative outcomes, drugmakers are striving to develop treatments that are easy to manufacture and can reach much larger numbers of patients.
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Sls1 and Mtf2 mediate the assembly of the Mrh5C complex required for activation of cox1 mRNA translation J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-16 Yirong Wang, Ting Jin, Ying Huang
Mitochondrial translation depends on mRNA-specific activators. In , DEAD-box protein Mrh5, pentatricopeptide repeat (PPR) protein Ppr4, Mtf2, and Sls1 form a stable complex (designated Mrh5C) required for translation of mitochondrial DNA (mtDNA)‐encoded mRNA, the largest subunit of the cytochrome oxidase complex. However, how Mrh5C is formed and what role Mrh5C plays in mRNA translation have not been
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Convergent mechanism underlying the acquisition of vertebrate scotopic vision J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-16 Keiichi Kojima, Masataka Yanagawa, Yasushi Imamoto, Yumiko Yamano, Akimori Wada, Yoshinori Shichida, Takahiro Yamashita
High sensitivity of scotopic vision (vision in dim light conditions) is achieved by the rods’ low background noise, which is attributed to a much lower thermal activation rate () of rhodopsin compared with cone pigments. Frogs and nocturnal geckos uniquely possess atypical rods containing noncanonical cone pigments that exhibit low , mimicking rhodopsin. Here, we investigated the convergent mechanism
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Truncation of the constant domain drives amyloid formation by immunoglobulin light chains J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-16 Francesca Lavatelli, Antonino Natalello, Loredana Marchese, Diletta Ami, Alessandra Corazza, Sara Raimondi, Maria Chiara Mimmi, Silvia Malinverni, P. Patrizia Mangione, Manel Terrones Palmer, Alessio Lampis, Monica Concardi, Guglielmo Verona, Diana Canetti, Eloisa Arbustini, Vittorio Bellotti, Sofia Giorgetti
AL amyloidosis is a life-threatening disease caused by deposition of immunoglobulin light chains. Whilst the mechanisms underlying light chains amyloidogenesis remain unclear, several studies have highlighted the role that tissue environment and structural amyloidogenicity of individual light chains have in the disease pathogenesis.
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Identification of PS1/gamma-secretase and glutamate transporter GLT-1 interaction sites J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-16 Florian Perrin, Priyanka Sinha, Shane Patrick Clancy Mitchell, Michael Sadek, Masato Maesako, Oksana Berezovska
The recently discovered interaction between Presenilin 1 (PS1), a catalytic subunit of γ-secretase responsible for generating amyloid-β (Aβ) peptides, and GLT-1, a major glutamate transporter in the brain (EAAT2) provides a mechanistic link between these two key factors involved in Alzheimer’s disease (AD) pathology. Modulating this interaction can be crucial to understand the consequence of such crosstalk
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Accessing natural vaccine adjuvants Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-15 Vincent Courdavault, Nicolas Papon
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Deciphering the role of Enterococcus faecium cytidine deaminase in gemcitabine resistance of gallbladder cancer J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-15 Lin Jiang, Lingxiao Zhang, Yijun Shu, Yuhan Zhang, Lili Gao, Shimei Qiu, Wenhua Zhang, Wenting Dai, Shili Chen, Ying Huang, Yingbin Liu
Gemcitabine-based chemotherapy is a cornerstone of standard care for gallbladder cancer (GBC) treatment. Still, drug resistance remains a significant challenge, influenced by factors such as tumor-associated microbiota impacting drug concentrations within tumors. , a member of tumor-associated microbiota, was notably enriched in the GBC patient cluster. In this study, we investigated the biochemical
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Human antigen R transfers miRNA to Syntaxin 5 to Synergize miRNA Export from Activated Macrophages J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-15 Sourav Hom Choudhury, Shreya Bhattacharjee, Kamalika Mukherjee, Suvendra N. Bhattacharyya
Intercellular miRNA exchange acts as a key mechanism to control gene expression post-transcriptionally in mammalian cells. Regulated export of repressive miRNAs allows the expression of inflammatory cytokines in activated macrophages. Intracellular trafficking of miRNAs from endoplasmic reticulum to endosomes is a rate determining step in the miRNA export process and plays an important role in controlling
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Exploring the IRE1 interactome: from canonical signaling functions to unexpected roles J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-15 Simon Le Goupil, Hadrien Laprade, Marc Aubry, Eric Chevet
The unfolded protein response (UPR) is a mechanism aiming at restoring endoplasmic reticulum (ER) homeostasis and is likely involved in other adaptive pathways. The UPR is transduced by three proteins acting as sensors and triggering downstream signaling pathways. Among them, IRE1α (referred to as IRE1 hereafter), an ER-resident type I transmembrane protein, exerts its function through both kinase
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Functional genomic screens with death rate analyses reveal mechanisms of drug action Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-13 Megan E. Honeywell, Marie S. Isidor, Nicholas W. Harper, Rachel E. Fontana, Gavin A. Birdsall, Peter Cruz-Gordillo, Sydney A. Porto, Madison Jerome, Cameron S. Fraser, Kristopher A. Sarosiek, David A. Guertin, Jessica B. Spinelli, Michael J. Lee
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Glia-enriched cortical organoids implanted in mice capture astrocyte diversity Nat. Biotechnol. (IF 46.9) Pub Date : 2024-03-14
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Differences between bacteria and eukaryotes in clamp loader mechanism, a conserved process underlying DNA replication J. Biol. Chem. (IF 5.5) Pub Date : 2024-03-14 Jacob T. Landeck, Joshua Pajak, Emily K. Norman, Emma L. Sedivy, Brian A. Kelch
Clamp loaders are pentameric ATPases that place circular sliding clamps onto DNA, where they function in DNA replication and genome integrity. The central activity of a clamp loader is the opening of the ring-shaped sliding clamp, and the subsequent binding to primer-template (p/t)-junctions. The general architecture of clamp loaders is conserved across all life, suggesting that their mechanism is
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De novo-designed transmembrane proteins bind and regulate a cytokine receptor Nat. Chem. Biol. (IF 14.8) Pub Date : 2024-03-13 Marco Mravic, Li He, Huong T. Kratochvil, Hailin Hu, Sarah E. Nick, Weiya Bai, Anne Edwards, Hyunil Jo, Yibing Wu, Daniel DiMaio, William F. DeGrado