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State‐of‐the‐art in transposable element modulation affected by drugs in malignant prostatic cancer cells J. Cell. Biochem. (IF 4.0) Pub Date : 2024-03-19 Anna Terrazzan, Riccardo Vanini, Pietro Ancona, Nicoletta Bianchi, Cristian Taccioli, Gianluca Aguiari
Over recent years, the investigation of transposable elements (TEs) has granted researchers a deeper comprehension of their characteristics and functions, particularly regarding their significance in the mechanisms contributing to cancer development. This manuscript focuses on prostate carcinoma cell lines and offers a comprehensive review intended to scrutinize the associations and interactions between
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Neuregulin 4 (Nrg4) cooperates with melatonin to regulate the PRL expression via ErbB4/Erk signaling pathway as a potential prolactin (PRL) regulator J. Cell. Biochem. (IF 4.0) Pub Date : 2024-03-11 Wen‐wen Lin, Guan‐yong Ou, Hui‐fang Dai, Wei‐jiang Zhao
Neuregulin‐4 (Nrg4) and melatonin play vital roles in endocrine diseases. However, there is little discussion about the function and potential mechanism of Nrg4 and melatonin in prolactin (PRL) regulation. The human normal pituitary data from Gene Expression Profiling Interactive Analysis (GEPIA) database was used to explore the correlation between NRG4 and PRL. The expression and correlation of NRG4
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Roles of PD‐L1 in human adipose‐derived mesenchymal stem cells under inflammatory microenvironment J. Cell. Biochem. (IF 4.0) Pub Date : 2024-03-07 Jinqiu Sun, Hannah Zhong, Bo Kang, Trenton Lum, Dongxue Liu, Shengxian Liang, Jijun Hao, Rui Guo
Mesenchymal stem cells (MSCs) display unique homing and immunosuppression features which make them promising candidates for cell therapy in inflammatory disorders. It is known that C‐X‐C chemokine receptor type 4 (CXCR4, also known as CD184) is a critical receptor implicated in MSCs migration, and the protein programmed death ligand‐1 (PD‐L1) is involved in MSC's immunosuppression. However, it remains
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AP‐1/C‐FOS and AP‐1/FRA2 differentially regulate early and late adipogenic differentiation of mesenchymal stem cells J. Cell. Biochem. (IF 4.0) Pub Date : 2024-03-05 Ganesh Suraj Bose, Garima Kalakoti, Abhijeet P. Kulkarni, Smriti Mittal
Obesity is defined as an abnormal accumulation of adipose tissue in the body and is a major global health problem due to increased morbidity and mortality. Adipose tissue is made up of adipocytes, which are fat‐storing cells, and the differentiation of these fat cells is known as adipogenesis. Several transcription factors (TFs) such as CEBPβ, CEBPα, PPARγ, GATA, and KLF have been reported to play
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InR and Pi3K maintain intestinal homeostasis through STAT/EGFR and Notch signaling in enteroblasts J. Cell. Biochem. (IF 4.0) Pub Date : 2024-03-04 Jiewei Wang, Hongmei Xue, Xinyu Yi, Hyonil Kim, Yangguang Hao, Li Hua Jin
To maintain the integrity of the adult gut, the proliferation and differentiation of stem cells must be strictly controlled. Several signaling pathways control the proliferation and differentiation of Drosophila intestinal epithelial cells. Although the modulatory effects of insulin pathway components on cell proliferation have been characterized, their specific role in which cell type and how these
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Induction of stearoyl-CoA desaturase confers cell density-dependent ferroptosis resistance in melanoma J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-16 Hitomi Shirahama, Yuri Tani, Satomi Tsukahara, Yuka Okamoto, Akiko Hasebe, Tomomiki Noda, Shuji Ando, Masaru Ushijima, Masaaki Matsuura, Akihiro Tomida
Ferroptosis is a form of regulated cell death that is induced by inhibiting glutathione peroxidase 4 (GPX4), which eliminates lipid peroxidation. Ferroptosis induction is influenced by the cell environment. However, the cellular states altering ferroptosis susceptibility remain largely unknown. We found that melanoma cell lines became resistant to ferroptosis as cell density increased. Comparative
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Novel oncogenic transcriptional targets of mutant p53 in esophageal squamous cell carcinoma J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-15 Sara A. George, Viswakalyan Kotapalli, Pandilla Ramaswamy, Raju Kumar, Swarnalata Gowrishankar, Shantveer G. Uppin, Murali D. Bashyam
Missense mutations in the DNA binding domain of p53 are observed frequently in esophageal squamous cell carcinoma (ESCC). Recent studies have revealed the potentially oncogenic transcriptional networks regulated by mutant p53 proteins. However, majority of these studies have focused on common “hotspot” p53 mutations while rarer mutations are poorly characterized. In this study, we report the characterization
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Identification of putative antiviral bioactive compounds derived from family Asteraceae: An in silico approach J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-19 Swati Srivastava, Mohd Shahnawaz Khan, Saheem Ahmad, Amit Dubey, Vijay Laxmi Saxena, Mohammad Haneef
This computational study investigates 21 bioactive compounds from the Asteraceae family as potential inhibitors targeting the Spike protein (S protein) of SARS‐CoV‐2. Employing in silico methods and simulations, particularly CDOCKER and MM‐GBSA, the study identifies two standout compounds, pterodontic acid and cichoric acid, demonstrating robust binding affinities (−46.1973 and −39.4265 kcal/mol) against
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The molecular circadian rhythms regulating the cell cycle J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-19 Qin Zhou, Ruohan Wang, Yunxia Su, Bowen Wang, Yunfei Zhang, Ximing Qin
The circadian clock controls the expression of a large proportion of protein‐coding genes in mammals and can modulate a wide range of physiological processes. Recent studies have demonstrated that disruption or dysregulation of the circadian clock is involved in the development and progression of several diseases, including cancer. The cell cycle is considered to be the fundamental process related
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Continuous genetic monitoring of transient mesenchymal gene activities in distal tubule and collecting duct epithelial cells during renal fibrosis J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-19 Zihang Xu, Shaotong Zhang, Tingting Han, Letong Cai, Simin Zhong, Xiaojie Yang, Shaohua Zhang, Yan Li, Kuo Liu, Bin Zhou, Xueying Tian
Epithelial cells (ECs) have been proposed to contribute to myofibroblasts or fibroblasts through epithelial‐mesenchymal transition (EMT) during renal fibrosis. However, since EMT may occur dynamically, transiently, and reversibly during kidney fibrosis, conventional lineage tracing based on Cre‐loxP recombination in renal ECs could hardly capture the transient EMT activity, yielding inconsistent results
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Selection of internalizing RNA aptamers into human breast cancer cells derived from primary sites J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-19 Pricila da Silva Cunha, Marcelo Coutinho de Miranda, Mariane Izabella Abreu de Melo, Andrea da Fonseca Ferreira, Joana Lobato Barbosa, Junnia Alvarenga de Carvalho Oliveira, Tércio de Souza Goes, Dawidson Assis Gomes, Alfredo Miranda de Goes
Breast cancer is the most common cancer in women. Although chemotherapy is still broadly used in its treatment, adverse effects remain a challenge. In this scenario, aptamers emerge as a promising alternative for theranostic applications. Studies using breast cancer cell lines provide useful information in laboratory and preclinical investigations, most of which use cell lines established from metastatic
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Cannabidiol activates MAPK pathway to induce apoptosis, paraptosis, and autophagy in colorectal cancer cells J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-15 Na Young Kim, Chakrabhavi Dhananjaya Mohan, Gautam Sethi, Kwang Seok Ahn
Mitogen-activated protein kinase (MAPK) activation by natural compounds is known to be involved in the induction of apoptosis, paraptosis, and autophagy. Cannabidiol (CBD), a bioactive compound found in Cannabis sativa, is endowed with many pharmacological activities. We investigated the cytotoxic effect of CBD in a panel of colorectal cancer (CRC) cells (HT-29, SW480, HCT-116, and HCT-15). CBD induced
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BRG1 mediates epigenetic regulation of TNFα-induced CCL2 expression in oral tongue squamous cell carcinoma cells J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-13 Mingyan Xu, Xuemei Lu, Feixiang Zhu, Xue Sun, Hongfa Yao, Junling Zhang, Weishi Chen, Haohao Zhu, Fan Liu, Song Lin Shi, Xiaoling Deng
Strong evidence has indicated that upregulation of chemokine (CC motif) ligand-2 (CCL2) expression and the presence of an inflammatory tumor microenvironment significantly contribute to the migratory and invasive properties of oral squamous cell carcinoma, specifically oral tongue squamous cell carcinoma (OTSCC). However, the precise epigenetic mechanism responsible for enhanced CCL2 expression in
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Intrinsic factors behind long COVID: IV. Hypothetical roles of the SARS-CoV-2 nucleocapsid protein and its liquid–liquid phase separation J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-13 Ahmed Eltayeb, Faisal Al-Sarraj, Mona Alharbi, Raed Albiheyri, Ehab H. Mattar, Isam M. Abu Zeid, Thamer A. Bouback, Atif Bamagoos, Vladimir N. Uversky, Alberto Rubio-Casillas, Elrashdy M. Redwan
When the SARS-CoV-2 virus infects humans, it leads to a condition called COVID-19 that has a wide spectrum of clinical manifestations, from no symptoms to acute respiratory distress syndrome. The virus initiates damage by attaching to the ACE-2 protein on the surface of endothelial cells that line the blood vessels and using these cells as hosts for replication. Reactive oxygen species levels are increased
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Repurposing FDA-approved drugs to target malaria through inhibition of dihydrofolate reductase in the folate biosynthesis pathway: A prospective approach J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-12 Kanika Verma, Rini Chaturvedi, Ayush K. Lahariya, Anil K. Verma, Kristan A. Schneider, Anup R. Anvikar, Praveen K. Bharti
Dihydrofolate reductase (DHFR) is a ubiquitous enzyme that regulates the biosynthesis of tetrahydrofolate among various species of Plasmodium parasite. It is a validated target of the antifolate drug pyrimethamine (Pyr) in Plasmodium falciparum (Pf), but its clinical efficacy has been hampered due to the emergence of drug resistance. This has made the attempt to screen Food & Drug Administration-approved
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Mechanotransduction and epigenetic modulations of chromatin: Role of mechanical signals in gene regulation J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-12 Jagdish Mishra, Subhajit Chakraborty, Niharika, Ankan Roy, Soumen Manna, Tirthankar Baral, Piyasa Nandi, Samir K. Patra
Mechanical forces may be generated within a cell due to tissue stiffness, cytoskeletal reorganization, and the changes (even subtle) in the cell's physical surroundings. These changes of forces impose a mechanical tension within the intracellular protein network (both cytosolic and nuclear). Mechanical tension could be released by a series of protein–protein interactions often facilitated by membrane
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Helicobacter pylori CagA protein induces gastric cancer stem cell-like properties through the Akt/FOXO3a axis J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-08 Zheng-wei Chen, Zhe-bin Dong, Han-ting Xiang, Sang-sang Chen, Wei-ming Yu, Chao Liang
The presence of Helicobacter pylori (H. pylori) infection poses a substantial risk for the development of gastric adenocarcinoma. The primary mechanism through which H. pylori exerts its bacterial virulence is the cytotoxin CagA. This cytotoxin has the potential to induce inter-epithelial mesenchymal transition, proliferation, metastasis, and the acquisition of stem cell-like properties in gastric
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Identification of potent BRD4-BD1 inhibitors using classical and steered molecular dynamics based free energy analysis J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-05 Ashish Gupta, Rituraj Purohit
In the present work a combination of traditional and steered molecular dynamics based techniques were employed to identify potential inhibitors against the human BRD4 protein (BRD4- BD1); an established drug target for multiple illnesses including various malignancies. Quinoline derivatives that were synthesized in-house were tested for their potential as new BRD4-BD1 inhibitors. Initially molecular
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Palmitoylation is required for Sept8-204 and Sept5 to form vesicle-like structure and colocalize with synaptophysin J. Cell. Biochem. (IF 4.0) Pub Date : 2024-02-03 Huicong Liu, Rong Tan, Jia Tong, Shuo Wen, Can Wu, Muding Rao, Jiangli Zhu, Shiqian Qi, Eryan Kong
Sept8 is a vesicle associated protein and there are two typical transcriptional variants (Sept8-204 and Sept8-201) expressed in mice brain. Interestingly, the coexpression of Sept8-204/Sept5 induces the formation of small sized vesicle-like structure, while that of the Sept8-201/Sept5 produces large puncta. Sept8 is previously shown to be palmitoylated. Here it was further revealed that protein palmitoylation
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DON/DRP-104 as potent serine protease inhibitors implicated in SARS-CoV-2 infection: Comparative binding modes with human TMPRSS2 and novel therapeutic approach J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-29 Ernest Oduro-Kwateng, Mahmoud E. Soliman
Human transmembrane serine protease 2 (TMPRSS2) is an important member of the type 2 transmembrane serine protease (TTSP) family with significant therapeutic markings. The search for potent TMPRSS2 inhibitors against severe acute respiratory syndrome coronavirus 2 infection with favorable tissue specificity and off-site toxicity profiles remains limited. Therefore, probing the anti-TMPRSS2 potential
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In silico analysis of alpha-synuclein protein variants and posttranslational modifications related to Parkinson's disease J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-18 Aloma N. R. da Silva, Gabriel R. C. Pereira, Luiz Felippe Sarmento Bonet, Tiago Fleming Outeiro, Joelma F. De Mesquita
Parkinson's disease (PD) is among the most prevalent neurodegenerative disorders, affecting over 10 million people worldwide. The protein encoded by the SNCA gene, alpha-synuclein (ASYN), is the major component of Lewy body (LB) aggregates, a histopathological hallmark of PD. Mutations and posttranslational modifications (PTMs) in ASYN are known to influence protein aggregation and LB formation, possibly
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Metformin ameliorates mitochondrial damage induced by C9orf72 poly(GR) via upregulating AKT phosphorylation J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-17 Yiyuan Feng, Zhongyun Xu, Hongfu Jin, Yuanyuan Chen, Chenglai Fu, Yu Zhang, Yafu Yin, Hui Wang, Weiwei Cheng
Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are devastating neurodegenerative diseases with no effective cure. GGGGCC repeat expansion in C9orf72 is the most common genetic cause of both ALS and FTD. A key pathological feature of C9orf72 related ALS/FTD is the presence of abnormal dipeptide repeat proteins translated from GGGGCC repeat expansion, including poly Glycine-Arginine
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TBC1D2B undergoes phase separation and mediates autophagy initiation J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-16 Marina E. Hoffmann, Anne-Claire Jacomin, Doris Popovic, Daniel Kalina, Adriana Covarrubias-Pinto, Ivan Dikic
Small ubiquitin-like modifiers from the ATG8 family regulate autophagy initiation and progression in mammalian cells. Their interaction with LC3-interacting region (LIR) containing proteins promotes cargo sequestration, phagophore assembly, or even fusion between autophagosomes and lysosomes. Previously, we have shown that RabGAP proteins from the TBC family directly bind to LC3/GABARAP proteins. In
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Clock gene Per2 modulates epidermal tissue repair in vivo J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-16 Veronica Quispe Yujra, Ericka Janine Dantas da Silveira, Daniel Araki Ribeiro, Rogerio Moraes Castilho, Cristiane Helena Squarize
Wound healing can be influenced by genes that control the circadian cycle, including Per2 and BMAL1, which coordinate the functions of several organs, including the skin. The aim of the study was to evaluate the role of PER2 during experimental skin wound healing. Two groups (control and Per2-KO), consisting of 14 male mice each, were anesthetized by inhalation, and two 6 mm wounds were created on
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Pyruvate maintains and enhances the pro-inflammatory response of microglia caused by glucose deficiency in early stroke J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-16 Peng Zhou, Zhe-Cheng Yu, Cong Cao, Huai-Rui Cui, Mao-Chao Ding, Chao-Xian Yang, Min Liao
Pro-inflammatory microglia mainly rely on glycolysis to maintain cytokine production during ischemia, accompanied by an increase in inducible nitric oxide synthase (iNOS) and monocarboxylate transporter 1 (MCT1). The role of energy metabolism in the pro-inflammatory response of microglia is currently unclear. In this study, we tested the response of microglia in mice after cerebral ischemia and simulated
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Gastric cancer mesenchymal stem cells promote tumor glycolysis and chemoresistance by regulating B7H3 in gastric cancer cells J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-16 Qiuzhi Gao, Chao Huang, Ting Liu, Fumeng Yang, Zhihong Chen, Li Sun, Yuanyuan Zhao, Mei Wang, Liqi Luo, Chenglin Zhou, Wei Zhu
Despite surgical treatment combined with multidrug therapy having made some progress, chemotherapy resistance is the main cause of recurrence and death of gastric cancer (GC). Gastric cancer mesenchymal stem cells (GCMSCs) have been reported to be correlated with the limited efficacy of chemotherapy in GC, but the mechanism of GCMSCs regulating GC resistance needs to be further studied. The gene set
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The BCKDH kinase inhibitor BT2 promotes BCAA disposal and mitochondrial proton leak in both insulin-sensitive and insulin-resistant C2C12 myotubes J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-16 Caroline N. Rivera, Carly E. Smith, Lillian V. Draper, Madison E. Kee, Norah E. Cook, Macey R. McGovern, Rachel M. Watne, Andrew J. Wommack, Roger A. Vaughan
Elevated circulating branched-chain amino acids (BCAAs) have been correlated with the severity of insulin resistance, leading to recent investigations that stimulate BCAA metabolism for the potential benefit of metabolic diseases. BT2 (3,6-dichlorobenzo[b]thiophene-2-carboxylic acid), an inhibitor of branched-chain ketoacid dehydrogenase kinase, promotes BCAA metabolism by enhancing BCKDH complex activity
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Umbilical cord MSC-derived exosomes improve alveolar macrophage function and reduce LPS-induced acute lung injury J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-15 Enhai Cui, Lu Lv, Bin Wang, Liqin Li, Huadong Lu, Feng Hua, Wenyan Chen, Na Chen, Liwei Yang, Ruolang Pan
Acute lung injury (ALI) is a severe condition that can progress to acute respiratory distress syndrome (ARDS), with a high mortality rate. Currently, no specific and compelling drug treatment plan exists. Mesenchymal stem cells (MSCs) have shown promising results in preclinical and clinical studies as a potential treatment for ALI and other lung-related conditions due to their immunomodulatory properties
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Inhibition of receptor activator of nuclear factor kappa-B ligand-mediated osteoclast differentiation and bone resorption by Gryllus bimaculatus extract: An in vitro study J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-15 So Young Eun, Gyeong Do Park, Yoon-Hee Cheon, Myeung Su Lee, Hae Joong Cho, Ju-Young Kim
Excessive bone-resorbing osteoclast activity during bone remodeling is a major feature of bone diseases, such as osteoporosis. Therefore, the inhibition of osteoclast formation and bone resorption can be an effective therapeutic target for various bone diseases. Gryllus biomaculatus (GB) has recently been approved as an alternative food source because of its high nutritional value and environmental
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NAD+ supplementation prevents STING-induced senescence in CD8+ T cells by improving mitochondrial homeostasis J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-15 Bin Ye, Yingting Pei, Lujing Wang, Dehao Meng, Yu Zhang, Shuang Zou, Henian Li, Jinying Liu, Ziying Xie, Changhong Tian, Yuqi Jiang, Yu Qiao, Xu Gao, Yanfen Zhang, Ning Ma
Understanding the connection between senescence phenotypes and mitochondrial dysfunction is crucial in aging and premature aging diseases. Loss of mitochondrial function leads to a decline in T cell function, which plays a significant role in this process. However, more research is required to determine if improving mitochondrial homeostasis alleviates senescence phenotypes. Our research has shown
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The rational modulation of autophagy sensitizes colorectal cancer cells to 5-fluouracil and oxaliplatin J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-15 Andréa Baldasso-Zanon, Andrew Oliveira Silva, Nayara Franco, Rafael V. Picon, Guido Lenz, Patrícia Luciana da Costa Lopez, Eduardo C. Filippi-Chiela
Colorectal cancer (CRC) is the third most common and deadliest cancer globally. Regimens using 5-fluorouracil (5FU) and Oxaliplatin (OXA) are the first-line treatment for CRC, but tumor recurrence is frequent. It is plausible to hypothesize that differential cellular responses are triggered after treatments depending on the genetic background of CRC cells and that the rational modulation of cell tolerance
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Nonsynonymous mutations in VEGF receptor binding domain alter the efficacy of bevacizumab treatment J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-11 Ashif Ahamed, Arijit Samanta, Syed Sahajada Mahafujul Alam, Showkat Ahmad Mir, Zarnain Jamil, Safdar Ali, Mehboob Hoque
Vascular endothelial growth factor (VEGF) mediated angiogenesis is crucial for tumor progression. Isoforms of VEGF bind to different VEGF receptors (VEGFRs) to initiate angiogenesis specific cellular signaling. Inhibitors that target both the receptors and ligands are in clinical use to impede angiogenesis. Bevacizumab, a monoclonal antibody (mAb) approved by the Food and Drug Administration (FDA)
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Linc-ROR inhibits NK cell-killing activity by promoting RXRA ubiquitination and reducing MICB expression in gastric cancer patients J. Cell. Biochem. (IF 4.0) Pub Date : 2024-01-11 Qingbin Niu, Zongrui Li, He Jiang, Baoguang Hu
Linc-ROR plays an important role in gastric cancer (GC) development and progression. This study sought to determine how the aberrant expression of Linc-ROR impacts GC progression and immune evasion, and to identify new targets for GC therapy. GC cells overexpressing Linc-ROR and GSAGS cells were cocultured with NK-92 cells, respectively, and Linc-ROR expression was determined using reverse transcription
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Validating Fractalkine receptor as a target and identifying candidates for drug discovery against type 2 diabetes J. Cell. Biochem. (IF 4.0) Pub Date : 2023-12-19 Madhu Yadav, Yusuf Akhter
Type 2 diabetes mellitus (T2DM) is one of the most common chronic diseases employing abnormal levels of insulin. Enhancing the insulin production is greatly aided by the regulatory mechanisms of the Fractalkine receptor (CX3CR1) system in islet β-cell function. However, elements including a high-fat diet, obesity, and ageing negatively impact the expression of CX3CR1 in islets. CX3CL1/CX3CR1 receptor−ligand
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Circular RNA: A new expectation for cardiovascular diseases J. Cell. Biochem. (IF 4.0) Pub Date : 2023-12-14 Qiao Xie, Yun Ma, Zhong Ren, Tianhe Gu, Zhisheng Jiang
Circular RNA (circRNA) is a class of RNA with the 5' and 3' ends connected covalently to form a closed loop structure and characterized by high stability, conserved sequences and tissue specificity, which is caused by special reverse splicing methods. Currently, it has become a hot spot for research. With the discovery of its powerful regulatory functions and roles, the molecular mechanisms and future
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Intrinsic factors behind long COVID: III. Persistence of SARS-CoV-2 and its components J. Cell. Biochem. (IF 4.0) Pub Date : 2023-12-14 Nawal Abd El-Baky, Amro A. Amara, Vladimir N. Uversky, Elrashdy M. Redwan
Considerable research has been done in investigating SARS-CoV-2 infection, its characteristics, and host immune response. However, debate is still ongoing over the emergence of post-acute sequelae of SARS-CoV-2 infection (PASC). A multitude of long-lasting symptoms have been reported several weeks after the primary acute SARS-CoV-2 infection that resemble several other viral infections. Thousands of
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Asperosaponin VI facilitates the regeneration of skeletal muscle injury by suppressing GSK-3β-mediated cell apoptosis J. Cell. Biochem. (IF 4.0) Pub Date : 2023-12-11 Xinru Yang, Jian Liang, Yue Shu, Linlin Wei, Cailing Wen, Hui Luo, Liqing Ma, Tian Qin, Bin Wang, Siyu Zeng, Ying Liu, Chun Zhou
Asperosaponin VI (ASA VI) is a bioactive triterpenoid saponin extracted from Diptychus roots, of Diptyl, and has previously shown protective functions in rheumatoid arthritis and sepsis. This study investigates the effects and molecular mechanisms of ASA VI on skeletal muscle regeneration in a cardiotoxin (CTX)-induced skeletal muscle injury mouse model. Mice were subjected to CTX-induced injury in
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Novel mutation in EFEMP1 identified from two Chinese POAG families differentially activated endoplasmic reticulum stress markers and induced glaucoma in mouse J. Cell. Biochem. (IF 4.0) Pub Date : 2023-12-11 Xiaoqiang Xiao, Chong-Bo Chen, Zhenggen Wu, Yuhang Ye, Fang Deng, Yingjie Cao, Pingting Liu, Mingzhi Zhang
Primary open-angle glaucoma (POAG) is the most common type of glaucoma. Using whole-exome sequencing, we identified two independent families diagnosed as POAG from the China with a novel EFEMP1 variant (Exon3, c.175A>C p.Met59Leu); Three previously reported variants c.1160G>A p.R387Q, c.1189T>C p.Y397H, and c.1429C>T p.R477C in EFEPM1 from 55 sporadic POAG individuals were also identified. The variant
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From nature to cancer therapy: Evaluating the Streptomyces clavuligerus secondary metabolites for potential protein kinase inhibitors J. Cell. Biochem. (IF 4.0) Pub Date : 2023-12-04 Ravi Saini, Sonali Kumari, Amit Singh, Abha Mishra
The study aimed to evaluate the antioxidant, protein kinase inhibitory (PKIs) potential, cytotoxicity activity of Streptomyces clavuligerus extract. DPPH assay revealed a robust free radical scavenging capacity (IC50 28.90 ± 0.24 µg/mL) of organic extract with a maximum inhibition percentage of 61 ± 1.04%. PKIs assay revealed the formation of a whitish bald zone by S. clavuligerus extracts which indicates
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ZNF746 plays cardinal roles on colorectal cancer (CRC) cell invasion and migration and regulates mitochondrial dynamics and morphological changes of CRC cells—Role of combined melatonin and 5-FU regimen J. Cell. Biochem. (IF 4.0) Pub Date : 2023-12-04 Chi-Ruei Huang, Yu-Ting Chu, Chia-Lo Chang, Hon-Kan Yip, Hong-Hwa Chen
The underlying mechanism of colorectal cells developing into cancer cells has been extensively investigated, yet is still not fully delineated, resulting in the treatment of advanced colorectal cancer (CRC) remains regrettably an unmet need. Zinc Finger Protein 746/Parkin-interacting substrate (ZNF746/PARIS) has previously been identified to play a fundamental role on bladder cancer cell proliferation
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In silico and structure-based evaluation of deleterious mutations identified in human Chk1, Chk2, and Wee1 protein kinase J. Cell. Biochem. (IF 4.0) Pub Date : 2023-12-04 Venessa Colaco, Nabajyoti Goswami, Vijay Kumar Goel, Sunil Kumar Srivastava, Pachuau Lalrohlua, Nachimuthu Senthil Kumar, Probodh Borah, Reshita Baruah, Ashok K. Varma
Checkpoint kinases Chk1, Chk2, Wee1 are playing a key role in DNA damage response and genomic integrity. Cancer-associated mutations identified in human Chk1, Chk2, and Wee1 were retrieved to understand the function associated with the mutation and also alterations in the folding pattern. Therefore, an attempt has been made to identify deleterious effect of variants using in silico and structure-based
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Koumine inhibits RANKL-induced ubiquitination and NF-κB activation to prevent ovariectomy and aging-induced bone loss J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-30 Jiongming You, Dingjun Xu, Chenxi Zhang, Yilin Chen, Song Huang, Huihui Bian, Juan Lv, Dagui Chen, Li Su, Heng Yin, Yinghua Li, Yong Wang
Osteoporosis (OP) is a bone remodeling disease characterized by an imbalance between bone resorption and formation. Osteoclasts are the primary therapeutic targets for treating bone destruction. Koumine (KM), the most bioactive component in Gelsemium alkaloids, exhibits antitumor, immunosuppressive, anti-inflammatory, and analgesic properties. However, the effects of bone loss have not been well studied
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The dyeing effect of acridine orange for multiple plasmid systems is sensitive to temperature J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-27 Qiuxia Jiao, Yumeng Zhang, Juan Xie, Fang Liu, Chaoming Peng, Qu Pan
The Goldview dyeing of the natural multiplasmid system of Lactobacillus plantarum PC518 was affected by temperature. The article want to identify the specific molecules that cause temperature sensitivity, then experiment on the universality of temperature sensitivity, and finally preliminarily analyze the influencing factors. At 5°C and 25°C, single pDNA, multiplasmid system, and linear DNA samples
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Predominant factors influencing reactive oxygen species in cancer stem cells J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-24 Loshini Soundararajan, Sudha Warrier, Arun Dharmarajan, Natarajan Bhaskaran
Reactive oxygen species (ROS) and its related signaling pathways and regulating molecules play a major role in the growth and development of cancer stem cells. The concept of ROS and cancer stem cells (CSCs) has been gaining much attention since the past decade and the evidence show that these CSCs possess robust self-renewal and tumorigenic potential and are resistant to conventional chemo- and radiotherapy
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Synergistic effect and molecular mechanism of PVA and UM171 in ex vivo expansion of primitive hematopoietic stem cells J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-22 Yan Ren, Yanni Cui, Jingyi Feng, Yanhong Tan, Fanggang Ren, Yaofang Zhang, Hongwei Wang
Umbilical cord blood (UCB) is a valuable source of hematopoietic stem cells (HSCs) used for transplantation; the number of cells in a single UCB is too small to quickly establish bone marrow (BM) implantation, and ex vivo expansion of HSCs has the potential to overcome this limitation. The purpose of this study is to explore the culture conditions conducive to the maintenance and expansion of hematopoietic
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A rationally engineered small antimicrobial peptide with potent antibacterial activity J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-22 Lalita Mohan Behera, Manaswini Ghosh, Pulkit Kr. Gupta, Soumendra Rana
Antimicrobial resistance (AMR) is a silent pandemic declared by the WHO that requires urgent attention in the post-COVID world. AMR is a critical public health concern worldwide, potentially affecting people at different stages of life, including the veterinary and agriculture industries. Notably, very few new-age antimicrobial agents are in the current developmental pipeline. Thus, the design, discovery
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OGT-1 regulates synaptic assembly through the insulin signaling pathway J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-22 Mengting Wu, Huihui Jiang, Qian Li, Yunhe Liu, Hongjun Zhang, Xuekun Li, Zhiyong Shao
The formation and maintenance of synapses are precisely regulated, and the misregulation often leads to neurodevelopmental or neurodegenerative disorders. Besides intrinsic genetically encoded signaling pathways, synaptic structure and function are also regulated by extrinsic factors, such as nutrients. O-GlcNAc transferase (OGT), a nutrient sensor, is abundant in the nervous system and required for
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Thyroid hormone receptor Thra and Thrb knockout differentially affects osteoblast biology and thyroid hormone responsiveness in vitro J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-22 Franziska Lademann, Elena Tsourdi, Lorenz C. Hofbauer, Martina Rauner
Thyroid hormones (TH) are important modulators of bone remodeling and thus, thyroid diseases, in particular hyperthyroidism, are able to compromise bone quality and fracture resistance. TH actions on bone are mediated by the thyroid hormone receptors (TR) TRα1 and TRβ1, encoded by Thra and Thrb, respectively. Skeletal phenotypes of mice lacking Thra (Thra0/0) and Thrb (Thrb−/−) are well-described and
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NUSAP1 regulates mouse oocyte meiotic maturation J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-22 Lina Yu, Na Kong, Yuling Lin, Panpan Qiu, Qian Xu, Yang Zhang, Xin Zhen, Guijun Yan, Haixiang Sun, Jie Mei, Guangyi Cao
The correct assembly of the spindle apparatus directly regulates the precise separation of chromosomes in mouse oocytes, which is crucial for obtaining high-quality oocytes capable of successful fertilization. The localization, assembly, migration, and disassembly of the spindle are regulated by a series of spindle-associated proteins, which exhibit unique expression level variations and specific localization
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Epithelial-like transport of mineral distinguishes bone formation from other connective tissues J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-22 Harry C. Blair, Quitterie C. Larrouture, Irina L. Tourkova, Deborah J. Nelson, Steven F. Dobrowolski, Paul H. Schlesinger
We review unique properties of bone formation including current understanding of mechanisms of bone mineral transport. We focus on formation only; mechanism of bone degradation is a separate topic not considered. Bone matrix is compared to other connective tissues composed mainly of the same proteins, but without the specialized mechanism for continuous transport and deposition of mineral. Indeed other
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2α, 3α, 24-Thrihydroxyurs-12-en-24-ursolic acid enhances the cytotoxic effect of cisplatin on oral cancer cells by down-regulating autophagy J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-22 Wentao Zhang, Ruijie Lu, Leyao Lv, Chenxi Ma, Yude Ding, Fan Yang, Qingxia Fang, Yue Wu, Ruolang Pan, Yunfang Chen
This study aimed to investigate the effect and mechanism of 2α, 3α, 24-thrihydroxyurs-12-en-24-ursolic acid (TEOA) alone or in combination with cisplatin on oral cancer. TEOA, a pentacyclic triterpenoid compound isolated from the roots of Actinidia eriantha, has demonstrated antitumor activity in preclinical experiments. However, its role in oral cancer remains poorly understood. Our findings revealed
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Intrinsically disordered proteins and liquid–liquid phase separation in SARS-CoV-2 interactomes J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-22 Lazar M. Vasović, Gordana M. Pavlović-Lažetić, Jovana J. Kovačević, Miloš V. Beljanski, Vladimir N. Uversky
This paper discusses the properties of proteins and their relations in the interactomes of the selected subsets of SARS-CoV-2 proteome—the membrane protein, nonstructural proteins, and, finally, full proteome. Protein disorder according to several measures, liquid–liquid phase separation probabilities, and protein node degrees in the interaction networks were singled out as the features of interest
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Ursolic acid regulates key EMT transcription factors, induces cell cycle arrest and apoptosis in MDA-MB-231 and MCF-7 breast cancer cells, an in-vitro and in silico studies J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-22 Venkataswamy Mallepogu, Karunakaran Reddy Sankaran, Chiranjeevi Pasala, Lokesh Reddy Bandi, Rajasekhar Maram, Uma Maheswari Amineni, Balaji Meriga
Epithelial–mesenchymal transition (EMT) is a vital process in tumorigenesis and metastasis of breast cancer. In our quest to explore effective anticancer alternatives, ursolic acid (UA) was purified from Capparis zeylanica and investigated for its anticancer activity against MDA-MB-231 and MCF-7 breast cancer cells. The apparent anticancer activity of UA on MDA-MB-231 and MCF-7 cells was evident from
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Bioinformatics insights into CENP-T and CENP-W protein-protein interaction disruptive amino acid substitution in the CENP-T-W complex J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-09 Suryakanta Mohanty, Rajendra Bhadane, Shashank Kumar
Kinetochores are multi-protein assemblies present at the centromere of the human chromosome and play a crucial role in cellular mitosis. The CENP-T and CENP-W chains form a heterodimer, which is an integral part of the inner kinetochore, interacting with the linker DNA on one side and the outer kinetochore on the other. Additionally, the CENP-T-W dimer interacts with other regulatory proteins involved
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Illuminating the structural basis of human neurokinin 1 receptor (NK1R) antagonism through classical all-atoms molecular dynamics simulations J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-09 Sarbani Mishra, Madhusmita Rout, Mahender Kumar Singh, Budheswar Dehury, Sanghamitra Pati
Advances in structural biology have bestowed insights into the pleiotropic effects of neurokinin 1 receptors (NK1R) in diverse patho-physiological processes, thereby highlighting the potential therapeutic value of antagonists directed against NK1R. Herein, we investigate the mode of antagonist recognition to discern the obscure atomic facets germane for the function and molecular determinants of NK1R
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Curcumin inhibits the activity and induces apoptosis of activated hepatic stellate cell by suppressing autophagy J. Cell. Biochem. (IF 4.0) Pub Date : 2023-11-01 Yongxiang Shu, Yajun He, Guorong Ye, Xuyou Liu, Jiahuang Huang, Qinghui Zhang, Da Tian, Tengyan Wang, Jianchang Shu
Curcumin, a kind of natural compound, has been previously proven to inhibit the autophagy in hepatic stellate cells (HSCs) and induce their apoptosis. However, it is not clear whether the enhanced apoptosis of activated HSCs (aHSCs) caused by curcumin depends on autophagy inhibition. We aim to verify this hypothesis and explore the potential mechanisms in this study. Immortalized human HSC line LX-2
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RNA N6-methyladenosine demethylase FTO inhibits glucocorticoid-induced osteoblast differentiation and function in bone marrow mesenchymal stem cells J. Cell. Biochem. (IF 4.0) Pub Date : 2023-10-26 Lingling Feng, Wei Zhao, Yunshan Fan, Chengcheng Yuan, Xiaohua Zhang
Excess glucocorticoids (GCs) have been reported as key factors that impair osteoblast (OB) differentiation and function. However, the role of RNA N6-methyladenosine (m6A) in this process has not yet been elucidated. In this study, we report that both the mRNA and protein expression of fat mass and obesity-associated gene (FTO), a key m6A demethylase, were dose-dependently downregulated during OB differentiation
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Machine learning-based antioxidant protein identification model: Progress and evaluation J. Cell. Biochem. (IF 4.0) Pub Date : 2023-10-25 Chaolu Meng, Yue Pei, Yongbo Bu, Quan Zou, Ying Ju
Efficient and accurate identification of antioxidant proteins is of great significance. In recent years, many models for identifying antioxidant proteins have been proposed, but the low sensitivity and high dimensionality of the models are common problems. The generalization ability of the model needs to be improved. Researchers have tried different feature extraction algorithms and feature selection
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PortPred: Exploiting deep learning embeddings of amino acid sequences for the identification of transporter proteins and their substrates J. Cell. Biochem. (IF 4.0) Pub Date : 2023-10-25 Marco Anteghini, Vitor AP Martins dos Santos, Edoardo Saccenti
The physiology of every living cell is regulated at some level by transporter proteins which constitute a relevant portion of membrane-bound proteins and are involved in the movement of ions, small and macromolecules across bio-membranes. The importance of transporter proteins is unquestionable. The prediction and study of previously unknown transporters can lead to the discovery of new biological