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Gene expression regulation by modulating Hfq expression in coordination with tailor-made sRNA-based knockdown in Escherichia coli J. Biotechnol. (IF 4.1) Pub Date : 2024-04-12 Yu Jung Jung, Keun Ha Park, Tae Yeong Jang, Seung Min Yoo
The tailor-made synthetic sRNA-based gene expression knockdown system has demonstrated its efficacy in achieving pathway balancing in microbes, facilitating precise target gene repression and fine-tuned control of gene expression. This system operates under a competitive mode of gene regulation, wherein the tailor-made synthetic sRNA shares the intrinsic intracellular Hfq protein with other RNAs. The
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Caproicibacter sp. BJN0012, a potential new species isolated from cellar mud for caproic acid production from glucose J. Biotechnol. (IF 4.1) Pub Date : 2024-04-12 Mengqi Dai, Youqiang Xu, Lei Zhao, Mengqin Wu, Huifeng Ma, Lining Zhu, Weiwei Li, Xiuting Li, Baoguo Sun
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Ectoine production from a novel bacterial strain and high-purity purification with a cost-effective and single-step method J. Biotechnol. (IF 4.1) Pub Date : 2024-04-09 Furkan Orhan, Akın Akıncıoğlu, Ertuğrul Ceyran
This study marks the exploration into the production of ectoine, a valuable compound with significant potential as an antioxidant, osmoprotectant, anti-inflammatory agent, and stabilizer of cell membranes, proteins, and DNA integrity. Our focus centred on investigating the presence of ectoine and optimizing its production by the novel ectoine producer bacterial strain, . For the optimization of ectoine
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Adding value to banana farming: Antibody production in post-harvest leaves J. Biotechnol. (IF 4.1) Pub Date : 2024-04-04 Jasdeep Kaur Darsan Singh, Purabi Mazumdar, Rofina Yasmin Othman, Jennifer Ann Harikrishna
Banana, a globally popular fruit, is widely cultivated in tropical and sub-tropical regions. After fruit harvest, remaining banana plant materials are low-value byproducts, mostly composted or used as fibre or for food packaging. As an aim to potentially increase farmer income, this study explored underutilised banana biomass as a novel plant tissue for production of a high-value product. Protein scFvTG130
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Purification, functional characterization and enhanced production of serratiopeptidase from Serratia marcescens MES-4: An endophyte isolated from Morus rubra J. Biotechnol. (IF 4.1) Pub Date : 2024-04-04 Diksha Koul, Devtulya Chander, Ravi S. Manhas, Md. Mehedi Hossain, Mohd Jamal Dar, Asha Chaubey
Serratiopeptidase, a proteolytic enzyme serves as an important anti-inflammatory and analgesic medication. Present study reports the production and purification of extracellular serratiopeptidase from an endophyte, MES-4, isolated from . Purification of the enzyme by Ion exchange chromatography led to the specific activity of 13,030 U/mg protein of serratiopeptidase, showcasing about 3.1 fold enhanced
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Reduction of anthranilic acid to 2-aminobenzaldehyde by the white-rot fungus Bjerkandera adusta DSMZ 4708 J. Biotechnol. (IF 4.1) Pub Date : 2024-04-04 Valeriia Babkina, Yana Haiduk, Yuliia Kurtash, Holger Zorn, Tatyana Zhuk
The biocatalytic aerobic “in-water” reduction of anthranilic acid to 2-aminobenzaldehyde by growing cultures of the basidiomycetous white-rot fungus has been studied. The high specific activity of towards the carboxylic group of anthranilic acid that allows avoiding the formation of the corresponding alcohol has been demonstrated using different substrate concentrations. The presence of ethanol as
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Enhancing the soluble expression of α-1,2-fucosyltransferase in E. coli using high-throughput flow cytometry screening coupled with a split-GFP J. Biotechnol. (IF 4.1) Pub Date : 2024-03-29 Jun-Min Lee, Jung Hwa Kim, Jin Young Kim, Min-Kyu Oh, Byung-Gee Kim
2’-Fucosyllactose (2’-FL), one of the major human milk oligosaccharides, was produced in several engineered microorganisms. However, the low solubility of α-1,2-fucosyltransferase (α1,2-FucT) often becomes a bottleneck to produce maximum amount of 2’-FL in the microorganisms. To overcome this solubility issue, the following studies were conducted to improve the soluble expression of α1,2-FucT. Initially
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Practical deployment of automation to expedite aqueous two-phase extraction J. Biotechnol. (IF 4.1) Pub Date : 2024-03-28 Mario A. Torres-Acosta, Alex Olivares-Molina, Ross Kent, Nuno Leitão, Markus Gershater, Brenda Parker, Gary J. Lye, Duygu Dikicioglu
The feasibility of bioprocess development relies heavily on the successful application of primary recovery and purification techniques. Aqueous two-phase extraction (ATPE) disrupts the definition of "unit operation" by serving as an integrative and intensive technique that combines different objectives such as the removal of biomass and integrated recovery and purification of the product of interest
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Modification of the endoplasmic reticulum morphology enables improved recombinant antibody expression in Saccharomyces cerevisiae J. Biotechnol. (IF 4.1) Pub Date : 2024-03-28 Laura R.K. Niemelä, Essi V. Koskela, Alexander D. Frey
The yeast is a versatile cell factory used for manufacturing of a wide range of products, among them recombinant proteins. Protein folding is one of the rate-limiting processes and this shortcoming is often overcome by the expression of folding catalysts and chaperones in the endoplasmic reticulum (ER). In this work, we aimed to establish the impact of ER structure on cellular productivity. The reticulon
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Enhanced prokaryotic expression, purification, and biological activities of human keratinocyte growth factor J. Biotechnol. (IF 4.1) Pub Date : 2024-03-27 Thi Luong Vu, Thi Kieu Oanh Nguyen, Jung-A. Song, Seonha Chong, Han Choe
Keratinocyte growth factor (KGF), also known as fibroblast growth factor 7 (FGF7), plays a critical role in embryonic development, cell proliferation, and differentiation. However, efficient production of recombinant KGF remains a challenge due to its low expression levels and high tendency for aggregation in . This study aimed to enhance the expression and solubility of KGF by employing different
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Assessment of the efficiency and stability of enzymatic membrane reaction utilizing lipase covalently immobilized on a functionalized hybrid membrane J. Biotechnol. (IF 4.1) Pub Date : 2024-03-26 Hosna Rezaie, Abolghasem Abbasi Kajani, Faranak Jafarian, Sina Asgari, Asghar Taheri-Kafrani, Abdol-Khalegh Bordbar
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Carotenoids production by Rhodosporidium paludigenum yeasts: Characterization of chemical composition, antioxidant and antimicrobial properties J. Biotechnol. (IF 4.1) Pub Date : 2024-03-26 Fani Sereti, Maria Alexandri, Aikaterini Papadaki, Harris Papapostolou, Nikolaos Kopsahelis
The high market potential imposed by natural carotenoids has turned the scientific interest in search for new strains, capable of synthesizing a wide spectrum of these pigments. In this study, NCYC 2663 and 2664 were investigated for carotenoids production and lipid accumulation utilizing different carbon sources (glucose, fructose, sucrose, mixture of glucose: galactose). Strain 2663 produced the
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Establishment of efficient 5-hydroxyvaleric acid production system by regenerating alpha-ketoglutaric acid and its application in poly(5-hydroxyvaleric acid) production J. Biotechnol. (IF 4.1) Pub Date : 2024-03-22 Suhye Choi, Byungchan Kim, Suwon Kim, Yeda Lee, Yuni Shin, Jinok Oh, Shashi Kant Bhatia, Seung-Oh Seo, See-Hyoung Park, Kyungmoon Park, Yung-Hun Yang
5-hydroxyvaleric acid (5-HV) is a versatile C5 intermediate of bio-based high-value chemical synthesis pathways. However, 5-HV production faces a few shortcomings involving the supply of cofactors, especially α-ketoglutaric acid (α-KG). Herein, we established a two-cell biotransformation system by introducing glutamate oxidase (GOX) to regenerate α-KG. Additionally, the catalase KatE was adapted to
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Engineering the activity and thermostability of a carboxylic acid reductase in the conversion of vanillic acid to vanillin J. Biotechnol. (IF 4.1) Pub Date : 2024-03-21 Yaoyao Ren, Zongmin Qin, Congcong Li, Bo Yuan, Yang Yang, Ge Qu, Zhoutong Sun
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Optimizing mycelial protein yield in Pleurotus djamor via ARTP mutagenesis and hybridization strategies J. Biotechnol. (IF 4.1) Pub Date : 2024-03-20 Jing Pan, Jian Zhang, Hefen Wei, Qingguo Liu, Wenhui Xu, Yunhui Bao
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Primary microglia cell cultures in translational research: Strengths and limitations J. Biotechnol. (IF 4.1) Pub Date : 2024-03-20 István Pesti, Ádám Légrádi, Eszter Farkas
Microglia are the resident macrophages in the central nervous system, accounting for 10–15% of the cell mass in the brain. Next to their physiological role in development, monitoring neuronal function and the maintenance of homeostasis, microglia are crucial in the brain’s immune defense. Brain injury and chronic neurological disorders are associated with neuroinflammation, in which microglia activation
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Reduced production of Ethyl Carbamate in wine by regulating the accumulation of arginine in Saccharomyces cerevisiae J. Biotechnol. (IF 4.1) Pub Date : 2024-03-19 Manman Gao, Wenyao Li, Linlin Fan, Chunhui Wei, Shuo Yu, Ru Chen, Lijuan Ma, Liping Du, Huiling Zhang, Weiming Yang
Ethyl carbamate (EC), a multisite carcinogenic compound, is naturally produced from urea and ethanol in alcoholic beverages. In order to reduce the content of EC in wine, the accumulation of arginine in was regulated by genetic modifying genes involved in arginine transport and synthesis pathways to reduce the production of urea. Knockout of genes encoding arginine permease (Can1p) and amino acid permease
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Heterologous production of (-)-geosmin in Saccharomyces cerevisiae J. Biotechnol. (IF 4.1) Pub Date : 2024-03-11 Dheeraj Madhukar Mahajan, Pramod Shankar Kumbhar, Rishi Jain
(-)-Geosmin has high demand in perfumes and cosmetic products for its earthy congenial aroma. The current production of (-)-geosmin is either by distillation of sun-baked soil or by inefficient chemical synthesis because of the presence of multiple chiral centers. Fermentation processes are not viable as the titers of the based processes are low. This work presents an alternative route by the heterologous
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In vitro synthesis of oryzamutaic acid H using recombinant L-lysine ε-dehydrogenase from Agrobacterium tumefaciens J. Biotechnol. (IF 4.1) Pub Date : 2024-03-11 Yuta Horie, Makoto Uchikata, Sachie Ueda, Gai Yamahira, Masaru Hashimoto, Kimitoshi Sakamoto, Hiroyuki Ito, Shigeki Hamada
Oryzamutaic acids, possessing a nitrogen-containing heterocyclic skeleton, have been isolated and identified from a rice mutant. Although oryzamutaic acids are expected to be functional ingredients, their functionality is difficult to evaluate, because of their wide variety and presence in trace amounts. Furthermore, how oryzamutaic acid is synthesized is unclear. Therefore, we developed a simple enzymatic
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Bioethanol Production from Characterized Pre-treated Sugarcane Trash and Jatropha Agrowastes J. Biotechnol. (IF 4.1) Pub Date : 2024-03-08 Naglaa A. Elnagdy, Tamer I.M. Ragab, Mohamed A. Fadel, Mohamed A. Abou-Zeid, Mona A. Esawy
Low production costs and a potential feedstock supply make lignocellulosic ethanol (bioethanol) an important source of advanced biofuels. The physical and chemical preparation of this kind of lignocellulosic feedstock led to a high ethanol yield. In order to increase the yield of fermentable sugars, pretreatment is an essential process step that alters the lignocellulosic structure and improves its
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Investigation of anti-adherence and antimicrobial properties of prodigiosin-functionalized bacterial cellulose membrane for biomedical applications J. Biotechnol. (IF 4.1) Pub Date : 2024-03-06 Sinem Diken-Gür
In this study, novel biomaterial that consisted entirely of bacterial products was developed with the approach of designing cost effective material for biomedical applications. With this aim, bacterial cellulose membranes (BCMs) which synthesized by were produced. Moreover, to impart antimicrobial properties to enhance the capacity of BCMs for biomedical usage, prodigiosin (PG) pigment of which presents
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Reviewing critical TRPM2 variants through a structure-function lens J. Biotechnol. (IF 4.1) Pub Date : 2024-03-03 Ádám V. Tóth, Ádám Bartók
The transient receptor potential melastatin 2 (TRPM2) channel plays a central role in connecting redox state with calcium signaling in living cells. This coupling makes TRPM2 essential for physiological functions such as pancreatic insulin secretion or cytokine production, but also allows it to contribute to pathological processes, including neuronal cell death or ischemia-reperfusion injury. Genetic
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Engineering carboxylic acid reductases and unspecific peroxygenases for flavor and fragrance biosynthesis J. Biotechnol. (IF 4.1) Pub Date : 2024-02-28 Alejandra Agosto-Maldonado, Jiantao Guo, Wei Niu
Emerging consumer demand for safer, more sustainable flavors and fragrances has created new challenges for the industry. Enzymatic syntheses represent a promising green production route, but the broad application requires engineering advancements for expanded diversity, improved selectivity, and enhanced stability to be cost-competitive with current methods. This review discusses recent advances and
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Understanding the mechanism of polyethyleneimine-mediated cell disintegration and protein extraction in E. coli: The role of floc network formation and PEI molecular weight J. Biotechnol. (IF 4.1) Pub Date : 2024-02-28 Alexander Jurjevec, Cécile Brocard, Gerald Striedner, Monika Cserjan-Puschmann, José L. Toca-Herrera, Rainer Hahn
Cell disintegration and protein extraction are crucial steps in downstream process development for biopharmaceuticals produced in . In this study, we explored the extraction mechanism of polyethyleneimine (PEI) at the cellular level and characterized the floc network that is formed upon PEI addition by Focused Beam Reflectance Measurement and Dispersion Analyzer. PEI disintegrates the cells by detachment
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Sorption properties of pristine and functionalized magnetic carbon nanotubes in relation to double-stranded DNA J. Biotechnol. (IF 4.1) Pub Date : 2024-02-28 Yanina Rezvantseva, Dmitry Zybin, Anna Prostyakova, Vladimir Oleinikov, Dmitry Kapustin
Owing to improvement of the molecular diagnostic methods using purified preparations of nucleic acids (NAs), the development of effective methods providing the isolation of DNA is still relevant. The sorption properties of magnetic multi-walled carbon nanotubes (MWCNTs), oxidized MWCNTs and MWCNTs (pristine and oxidized) modified with polydiallyldimethylammonium chloride (pDADMAC) with respect to double
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DNA digestion and formation of DNA-network structures with Holliday junction-resolving enzyme Hjc_15-6 in conjunction with polymerase reactions J. Biotechnol. (IF 4.1) Pub Date : 2024-02-24 Josefin Ahlqvist, Joanna Tymecka-Mulik, Katarzyna Burkiewicz, Reine Wallenberg, Andrius Jasilionis, Eva Nordberg Karlsson, Slawomir Dabrowski
The recently identified novel Holliday junction-resolving enzyme, termed Hjc_15-6, activity investigation results imply DNA cleavage by Hjc_15-6 in a manner that potentially enhances the molecular self-assembly that may be exploited for creating DNA-networks and nanostructures. The study also demonstrates DNA polymerase acting in combination with Hjc_15-6 capability to produce large amounts of DNA
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Enhancement of the biological activity of hydroxytyrosol through its oxidation by laccase from Trametes versicolor J. Biotechnol. (IF 4.1) Pub Date : 2024-02-23 Alexandra V. Chatzikonstantinou, Myrto G. Bellou, Stamatia Spyrou, Angelos Papanikolaou, Yannis V. Simos, Dimitrios Peschos, Haralambos Stamatis
The laccase-catalyzed oxidation of hydroxytyrosol (HT) towards the formation of its bioactive oligomer derivatives was investigated. The biocatalytic oligomerization was catalyzed by laccase from in aqueous or various water-miscible organic solvents and deep eutectic solvent (DES)-based media. Mass Spectroscopy and Nuclear Magnetic Resonance were used for the characterization of the products. The solvent
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Viral inactivation for pH-sensitive antibody formats such as multi-specific antibodies J. Biotechnol. (IF 4.1) Pub Date : 2024-02-23 Anaïs Duret, Lionel Duarte, Laure Cahuzac, Apolline Rondepierre, Monia Lambercier, Romain Mette, Achim Recktenwald, Roberto Giovannini, Martin Bertschinger
Recently developed multi-specific antibody formats enable new therapeutic concepts. Conveniently, formats with an Fc domain allow purification in well-established mAb platform processes. However, due to the structural complexity of the formats, the assembled molecules may be sensitive to extreme pH commonly used for viral inactivation. An alternative to low pH incubation for virus inactivation is the
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Improved biocatalytic activity of steapsin lipase in supercritical carbon dioxide medium for the synthesis of benzyl butyrate: A commercially important flavour compound J. Biotechnol. (IF 4.1) Pub Date : 2024-02-23 Kirtikumar C. Badgujar, Jagruti K. Badgujar, Bhalchandra M. Bhanage
Enzymatic synthesis of flavours, fragrances and food additives compounds have great demand and market value. Benzyl butyrate is commercially important flavour and food additive compound having global use around 100 metric tons/year and widely used in various industrial sectors. However, industrial synthesis of food additive benzyl butyrate is carried out by conventional chemical process which demands
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Saline extraction assisted by ultrasound: a method to obtain purified phycocyanin J. Biotechnol. (IF 4.1) Pub Date : 2024-02-22 Ingrid Rocha Teixeira, Ligia Damasceno Ferreira Marczak, Giovana Domeneghini Mercali, Débora Pez Jaeschke
The influence of ultrasound intensity and saline solution concentration (NHCl and CaCl) on phycocyanin extraction from was evaluated. The intensity had a significant effect on phycocyanin content and purity, while the saline solution concentration only had an effect on purity. The optimum extraction condition was obtained at 41% of intensity and 8.5 gL of CaCl solution. In this condition, ultrasound
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Nitrile-synthesizing enzymes and biocatalytic synthesis of volatile nitrile compounds: A review J. Biotechnol. (IF 4.1) Pub Date : 2024-02-21 Takuya Yamaguchi, Yasuhisa Asano
Nitriles (R−CN) comprise a broad group of chemicals industrially produced and used in fine chemicals, pharmaceuticals, and bulk applications, polymer chemistry, solvents, etc. Nitriles are important starting materials for producing carboxylic acids, amides, amines, and several other compounds. In addition, some volatile nitriles have been evaluated for their potential as ingredients in fragrance and
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Immobilization of aldoxime dehydratases on metal affinity resins and use of the immobilized catalysts for the synthesis of nitriles important in fragrance industry J. Biotechnol. (IF 4.1) Pub Date : 2024-02-17 Barbora Křístková, Ludmila Martínková, Lenka Rucká, Michael Kotik, Natalia Kulik, Robert Rädisch, Margit Winkler, Miroslav Pátek
Nitriles have a wide range of uses as building blocks, solvents, and alternative fuels, but also as intermediates and components of flavors and fragrances. The enzymatic synthesis of nitriles by aldoxime dehydratase (Oxd) is an emerging process with significant advantages over conventional approaches. Here we focus on the immobilization of His-tagged Oxds on metal affinity resins, an approach that
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Integrative analysis of the transcriptome and metabolome reveals Bacillus atrophaeus WZYH01-mediated salt stress mechanism in maize (Zea mays L.) J. Biotechnol. (IF 4.1) Pub Date : 2024-02-10 Yaling Hou, Wenzhi Zeng, Chang Ao, Jiesheng Huang
Maize is an important food crop that is affected by salt stress during growth, which can hinder plant growth and result in a significant decrease in yield. The application of plant growth-promoting rhizobacteria can improve this situation to a certain extent. However, the gene network of rhizosphere-promoting bacteria regulating the response of maize to salt stress remains elusive. Here, we used metabolomics
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Maduramycin, a novel glycosylation modulator for mammalian fed-batch and steady-state perfusion processes J. Biotechnol. (IF 4.1) Pub Date : 2024-02-09 Patrick Romann, Thomas Vuillemin, Silvia Pavone, Martin Jordan, Arnaud Perilleux, Jonathan Souquet, Jean-Marc Bielser, Christoph Herwig, Thomas K. Villiger
Controlling high-mannose (HM) content of therapeutic proteins during process intensification, reformulation for subcutaneous delivery, antibody-drug conjugate or biosimilar manufacturing represents an ongoing challenge. Even though a range of glycosylation levers to increase HM content exist, modulators specially increasing M5 glycans are still scarce. Several compounds of the polyether ionophore family
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SWATH-MS as a strategy for CHO host cell protein identification and quantification supporting the characterization of mAb purification platforms J. Biotechnol. (IF 4.1) Pub Date : 2024-02-08 Sofia B. Carvalho, Ludivine Profit, Sushmitha Krishnan, Ricardo A. Gomes, Bruno M. Alexandre, Severine Clavier, Michael Hoffman, Kevin Brower, Patrícia Gomes-Alves
Host cell proteins (HCPs) are process-related impurities expressed by the host cells during biotherapeutics’ manufacturing, such as monoclonal antibodies (mAbs). Some challenging HCPs evade clearance during the downstream processing and can be co-purified with the molecule of interest, which may impact product stability, efficacy, and safety. Therefore, HCP content is a critical quality attribute to
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RNA-based detection of genetically modified plants via current-voltage characteristic measurement J. Biotechnol. (IF 4.1) Pub Date : 2024-02-07 Chun-Kai Huang, Yi-Nan Lin, Wen-Shan Huang, Satyajyoti Senapati, Hsueh-Chia Chang, Yi-Ming Sun, Li-Fen Huang
The widespread adoption of genetically modified (GM) crops has escalated concerns about their safety and ethical implications, underscoring the need for efficient GM crop detection methods. Conventional detection methods, such as polymerase chain reaction, can be costly, lab-bound, and time-consuming. To overcome these challenges, we have developed RapiSense, a cost-effective, portable, and sensitive
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Cytokines as fast indicator of infectious virus titer during process development J. Biotechnol. (IF 4.1) Pub Date : 2024-02-05 Johanna Bacher, Narges Lali, Florian Steiner, Alois Jungbauer
Measuring infectious titer is the most time-consuming method during the production and process development of live viruses. Conventionally, it is done by measuring the tissue culture infectious dose (TCID50) or plaque forming units (pfu) in cell-based assays. Such assays require a time span of more than a week to the readout and significantly slow down process development. In this study, we utilized
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Construction and evaluation of a nanosystem that combines acidification promoted chemodynamic therapy and intracellular drug release monitoring J. Biotechnol. (IF 4.1) Pub Date : 2024-02-05 Kai Dong, Danyang Wang, Leiruo Lin, Peilin Niu, Yidong Wang, Qichao Tan, Jianfeng Xing
Triple-negative breast cancer (TNBC) is a highly invasive subtype of breast cancer that seriously affects women’s physical and mental health. Chemodynamic therapy (CDT) induces cell death by specifically generating Fenton/Fenton-like reactions within tumor cells. However, the weak acidity of the tumor microenvironment (TME) greatly weakens the effectiveness of CDT. This work constructed a kind of P-CAI/PT
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OptMSP: A Toolbox for Designing Optimal Multi-Stage (Bio)Processes J. Biotechnol. (IF 4.1) Pub Date : 2024-02-05 Jasmin Bauer, Steffen Klamt
One central goal of bioprocess engineering is to maximize the production of specific chemicals using microbial cell factories. Many bioprocesses are one-stage (batch) processes (OSPs), in which growth and product synthesis are coupled. However, OSPs often exhibit low volumetric productivities due to the competition for substrate for biomass and product synthesis implying trade-offs between biomass
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Interplay between desiccation and oxidative stress responses in iron-oxidizing acidophilic bacteria J. Biotechnol. (IF 4.1) Pub Date : 2024-02-03 Muñoz-Villagrán Claudia, Acevedo-Arbunic Javiera, Navarro-Salazar Sebastián, Fuentes-Rubio José, Levicán Gloria
Variations in water availability represent a foremost stress factor affecting the growth and survival of microorganisms. Acidophilic bioleaching bacteria are industrially applied for releasing metals from mineral sulphides, and they are considered extremely tolerant to oxidative conditions prevailing in acidic bioleaching environments. Such processes usually are performed in heaps and thus these microorganisms
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One-pot process for the biotransformation of vegetable oils into natural deca- and dodecalactones J. Biotechnol. (IF 4.1) Pub Date : 2024-01-29 Stefano Serra, Davide De Simeis
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The underlying mechanism of nuclear and mitochondrial DNA damages in triggering cancer incidences: Insights into proteomic and genomic sciences J. Biotechnol. (IF 4.1) Pub Date : 2024-02-02 Sami Saadi, Nor Elhouda Nacer, Nazamid Saari, Abdulkarim Sabo Mohammed, Farooq Anwar
The attempt of this review article is to determine the impact of nuclear and mitochondrial damages on the propagation of cancer incidences. This review has advanced our understanding to altered genes and their relevant cancerous proteins. The progressive raising effects of free reactive oxygen species ROS and toxicogenic compounds contributed to significant mutation in nuclear and mitochondrial DNA
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Investigation of cell infiltration and colonization in 3D porous scaffolds via integrated experimental and computational strategies J. Biotechnol. (IF 4.1) Pub Date : 2024-02-01 Yu Xiang, Jiongyi Yan, Xujin Bao, Andrew Gleadall, Tao Sun
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Predicting Drug Response of Small Cell Lung Cancer Cell Lines Based on Enrichment Analysis of Complex Gene Signatures J. Biotechnol. (IF 4.1) Pub Date : 2024-01-26 Kolos Nemes, Alexandra Benő, Petronella Topolcsányi, Éva Magó, Gabriella Mihalekné Fűr, Lőrinc S. Pongor
Advances in the field of genomics and transcriptomics have enabled researchers to identify gene signatures related to development and treatment of Small Cell Lung Cancer. In most cases, complex gene expression patterns are identified, comprising of genes with differential behavior. Most tools use single-genes as predictors of drug response, with only limited options for multi-gene use. Here we examine
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Carrier-based immobilization of Aerococcus viridans l-lactate oxidase J. Biotechnol. (IF 4.1) Pub Date : 2024-01-26 Christiane Luley-Goedl, Margherita Bruni, Bernd Nidetzky
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Cell-free reduction of carboxylic acids with secreted carboxylic acid reductase J. Biotechnol. (IF 4.1) Pub Date : 2024-01-22 Dominic Goj, Stella Ebner, Melissa Horvat, Simon Arhar, Ludmila Martínková, Margit Winkler
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Identifying and charactering a 4-aminobutyryl-CoA ligase for the production of butyrolactam J. Biotechnol. (IF 4.1) Pub Date : 2024-01-19 Xiaolin Shen, Xiaotian Jiang, Xinxiao Sun, Qipeng Yuan, Jia Wang
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pH-dependent electrostatic interactions between enzymes and nanoparticles in Pickering emulsions – Influence on activity and droplet size J. Biotechnol. (IF 4.1) Pub Date : 2024-01-19 Maximilian Seiler, Sebastian Stock, Anja Drews
Pickering emulsions (PE) are liquid-liquid systems that are stabilized by solid (nano)particles at the fluid interface. They offer higher stability, easier separation and lower toxicity compared to classical emulsions stabilized by surfactants. Common applications range from food science to drug delivery. In the last decade they have become of more interest in the field of multiphasic biocatalysis
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Improvement in organic solvent resistance of keratinase BLk by directed evolution J. Biotechnol. (IF 4.1) Pub Date : 2024-01-18 Fucheng Zhu, Zixu Yan, Jingli Dai, Guosi Li, Qilin Xu, Yunfeng Ma, Jingbo Ma, Naidong Chen, Xinhong Zhang, Yongjun Zang
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Volatile organic compounds: A threat to the environment and health hazards to living organisms – A review J. Biotechnol. (IF 4.1) Pub Date : 2024-01-17 Anju Mangotra, Shailesh Kumar Singh
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Recent advances in DNA-based probes for photoacoustic imaging J. Biotechnol. (IF 4.1) Pub Date : 2024-01-09 Anusri Anil, Jyotsna Chaskar, Avinash B. Pawar, Abhishekh Tiwari, Atul Changdev Chaskar
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Regulation of hydraulic retention time on caproic acid production via two-phase anaerobic fermentation of Chinese cabbage waste with autopoietic electron donors J. Biotechnol. (IF 4.1) Pub Date : 2024-01-02 Ranran Chen, Xiaofeng Ji, Zhengang Chen, Liu Huang, Jiying Zhu
The effects of hydraulic retention time (HRT) on the performance of two-phase anaerobic fermentation for caproic acid production from Chinese cabbage waste (CCW) were investigated. In the electron donor phase, yeast was inoculated to achieve efficient autopoietic ethanol, providing electron donors for the chain elongation process. Shorter HRT led to drastic fluctuations in microorganisms, thus resulting
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Comparison of plant biostimulating properties of Chlorella sorokiniana biomass produced in batch and semi-continuous systems supplemented with pig manure or acetate J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Wendy A. Stirk, Péter Bálint, Jitka Široká, Ondřej Novák, Tamás Rétfalvi, Zoltán Berzsenyi, Jácint Notterpek, Zoltán Varga, Gergely Maróti, Johannes van Staden, Miroslav Strnad, Vince Ördög
Microalgae-derived biostimulants provide an eco-friendly biotechnology for improving crop productivity. The strategy of circular economy includes reducing biomass production costs of new and robust microalgae strains grown in nutrient-rich wastewater and mixotrophic culture where media is enriched with organic carbon. In this study, Chlorella sorokiniana was grown in 100 l bioreactors under sub-optimal
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Glycolate as alternative carbon source for Escherichia coli J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Sonja Höhmann, Tim Arik Briol, Nadine Ihle, Oliver Frick, Andreas Schmid, Bruno Bühler
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Enhanced catalytic activity and stability of lactate dehydrogenase for cascade catalysis of D-PLA by rational design J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Yufeng Zhou, Xiaolong Sun, Jiahuan Hu, Yingjie Miao, Xiangyu Zi, Xi Luo, Yongqian Fu
Serving as a vital medical intermediate and an environmentally-friendly preservative, D-PLA exhibits substantial potential across various industries. In this report, the urgent need for efficient production motivated us to achieve the rational design of lactate dehydrogenase and enhance catalytic efficiency. Surprisingly, the enzymatic properties revealed that a mutant enzyme, LrLDHT247I/D249A/F306W/A214Y
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Modification of the Loop Region Near the Substrate Tunnel to Alter the Hydrolytic Process of Dextranase J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Bingbing Xia, Zhiwei Li, Jinao Wei, Ganpeng Hu, Jingwen Yang, Hongbin Zhang, Xueqin Hu
Dextranases are hydrolases that exclusively catalyze the disruption of α−1,6 glycosidic bonds. A series of variant enzymes were obtained by comparing the sequences of dextranases from different sources and introducing sequence substitutions. A correlation was found between the number of amino acids in the 397–401 region and the hydrolytic process. When there were no more than 5 amino acids in the 397–401
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UV-ARTP compound mutagenesis breeding improves macrolactins production of Bacillus siamensis and reveals metabolism changes by proteomic J. Biotechnol. (IF 4.1) Pub Date : 2024-01-06 Lian Yu, Fei Li, Jie Ni, Xianling Qin, Junxiang Lai, Xinying Su, Zhe Li, Mengfei Zhang
Macrolactins are a type of compound with complex macrolide structure which mainly be obtained through microbiological fermentation now. They have excellent antifungal, antibacterial and antitumor activity. In order to improve macrolactins production, Bacillus siamensis YB304 was used as the research object, and a mutant Mut-K53 with stable genetic characters was selected by UV-ARTP compound mutagenesis