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Histone H4 asymmetrically dimethylated at arginine 3 (H4R3me2a), a mark of super-enhancers. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-11-27 Sadhana R N Sudhakar,Li Wu,Shrinal Patel,Athanasios Zovoilis,James R Davie
Histone H4 asymmetrically dimethylated at arginine 3 (H4R3me2a) is an active histone mark catalyzed by protein arginine methyltransferase 1 (PRMT1), a major arginine methyltransferase in vertebrates catalyzing asymmetric dimethylation of arginine. H4R3me2a stimulates the activity of lysine acetyltransferases such as CBP/p300 which catalyze the acetylation of H3K27, a mark of active enhancers, super-enhancers
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The role of BCL2L13 in Glioblastoma: Turning a need into a target. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-11-21 Joadi Jacobs,Rosa Iranpour,Amir Barzegar Behrooz,Simone C da Silva Rosa,Saeid Ghavami
Glioblastoma (GBM) is the most common aggressive central nervous system (CNS) cancer. GBM has a high mortality rate, with a median survival time of 12-15 months after diagnosis. A poor prognosis and a shorter life expectancy may result from resistance to standard treatments such as radiation and chemotherapy. Temozolomide (TMZ) has been the mainstay treatment for GBM, but unfortunately, there are high
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Optimized total RNA isolation from bovine sperm with enhanced sperm head lysis. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-11-10 Saurabh Tiwari,Abdallah Shahat,John Kastelic,Nehal Thakor,Jacob Thundathil
Increasing evidence of sperm RNA's role in fertilization and embryonic development has provided impetus for its isolation and thorough characterization. Sperm are considered tough-to-lyse cells, due to compact condensed DNA in sperm heads. Lack of consensus among bovine sperm RNA isolation protocols introduces experimental variability in transcriptome studies. Here, we describe an optimized method
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Protein arginine methyltransferase 1, a major regulator of biological processes. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-11-03 Sadhana R N Sudhakar,Shahper N Khan,Ariel Clark,Thordur Hendrickson-Rebizant,Shrinal Patel,Ted M Lakowski,James R Davie
Protein arginine methyltransferase 1 (PRMT1) is a major type I arginine methyltransferase that catalyzes the formation of monomethyl and asymmetric dimethylarginine in protein substrates. It was first identified to asymmetrically methylate histone H4 at the third arginine residue forming the H4R3me2a active histone mark. However, several protein substrates are now identified as being methylated by
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WWP2 binds to NKRF, enhances the NF-κB signaling, and promotes malignant phenotypes of acute myeloid leukemia cells. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-11-03 Hongjia Wang,Xin Lian,Kexin Wang,Shuye Wang
Acute myeloid leukemia (AML) is one of the hematological malignancies with a high recurrence rate. WW domain-containing E3 ubiquitin protein ligase 2 (WWP2) is identified as a pivotal regulator of tumor progression. This study aimed to assess the possible role of WWP2 in AML. Analysis of the GEPIA database indicated an elevated WWP2 expression in AML. We established stable WWP2-overexpressed or WWP2-silenced
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The m6A reader IGF2BP2 promotes the progression of esophageal squamous cell carcinoma cells by increasing the stability of OCT4 mRNA. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-11-02 Rong Zhao,Ting Li,Xinran Zhao,Ziyi Yang,Liying Ma,Xiaoxia Wang
Esophageal squamous cell carcinoma (ESCC) is a common malignancy with high morbidity and mortality. Insulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) serves as a reader of RNA m6A (N6 methyladenosine) modification to regulate gene expression at the post-transcriptional level. Emerging evidence suggests that IGF2BP2 plays critical roles in tumorigenesis and malignant development. However
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Early pigment spot segmentation and classification from iris cellular image analysis with explainable deep learning and multiclass support vector machine. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-10-31 Amjad Khan,Rabia Javed,Tariq Sadad,Saeed Ali Bahaj,Gabriel Avelino Sampedro,Mideth Abisado
Globally, retinal disorders impact thousands of individuals. Early diagnosis and treatment of these anomalies might halt their development and prevent many people from developing preventable blindness. Iris spot segmentation is critical due to acquiring iris cellular images that suffer from the off-angle iris, noise, and specular reflection. Most currently used iris segmentation techniques are based
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BioCanRx Summit for Cancer Immunotherapy 2022 Proceedings. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-10-23 Stacey N Lee,Victoria Hoskin,Céline M Laumont,Shannon Snelling,Lorenzo Lindo,Lou Bird,Vera Samarkina,Chantale Thurston,Grace Fox,Sarah Ivanco,Megan Mahoney,Jeanette E Boudreau,Sarah Nersesian
From 19 to 21 November 2022, BioCanRx held its first post-pandemic in-person Summit for Cancer Immunotherapy in Montreal, Canada. The meeting was well attended by patients, trainees, researchers, clinicians, and industry professionals, who came together to discuss the current state and future of biotherapeutics for cancer in Canada and beyond. Three plenaries, three keynote speakers, a lively debate
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RNA-binding protein NOVA1 promotes acute T-lymphocyte leukemia progression by stabilizing USP44 mRNA. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-10-10 Bin Zhang,Ruowen Sun,Min Gu,Zehui Jiang,Ye Wang,Linlin Zhang,Xiaoyang Liu,Zuofei Chi
Acute T-lymphocyte leukemia (T-ALL) is a malignant tumor disease. RNA-binding protein neotumor ventral antigen-1 (NOVA1) is highly expressed in bone marrow mononuclear cells of T-ALL patients, while the role of NOVA1 in T-ALL progression remains unknown. The gain- and loss-of-function studies for NOVA1 were performed in Jurkat and CCRF-CEM cells. NOVA1 overexpression promoted cell proliferation and
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The 3' terminal region of Zika virus RNA contains a conserved G-quadruplex and is unfolded by human DDX17. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-09-29 Darren Gemmill,Corey Nelson,Maulik Badmalia,Higor S Pereira,Liam Kerr,Michael T Wolfinger,Trushar R Patel
Zika virus (ZIKV) infection remains a worldwide concern, and currently, no effective treatments or vaccines are available. Novel therapeutics are an avenue of interest that could probe viral RNA-human protein communication to stop viral replication. One specific RNA structure, G-quadruplexes (G4s), possess various roles in viruses and all domains of life, including transcription and translation regulation
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SUMO-3 promotes the ubiquitin-dependent turnover of TRIM55. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-09-13 Nour-El-Houda Hammami,Natacha Mérindol,Mélodie B Plourde,Tara Maisonnet,Sophie Lebel,Lionel Berthoux
Human muscle-specific RING fingers (MURFs) are members of the tripartite motif (TRIM) family of proteins characterized by their C-terminal subgroup one signature domain. MURFs play a role in sarcomere formation and microtubule dynamics. It was previously established that some TRIMs undergo post-translational modification by small ubiquitin-like modifier (SUMO). In this study, we explored the putative
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Crosstalk between chromatin, chromosomes, and epigenetics. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-08-29 Alyssa Ialongo,Ssu-Yu Yeh,Ho Sung Rhee
The International Asilomar Chromatin, Chromosomes, and Epigenetics Conference was held online from 8 to 10 December 2022. Topics of this year's conference included chromosome dysregulation, genome integrity, nuclear organization, regulation of chromatin, epigenetics, transcription, and gene regulation in cell differentiation and disease. The meeting featured four keynote speakers, including Yamini
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LINE-1: an emerging initiator of cGAS-STING signalling and inflammation that is dysregulated in disease. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-08-29 Sabateeshan Mathavarajah,Graham Dellaire
The cGAS-STING (cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING)) axis integrates DNA damage and cellular stress with type I interferon (IFN) signalling to facilitate transcriptional changes underlying inflammatory stress responses. The cGAS-STING pathway responds to cytosolic DNA in the form of double-stranded DNA, micronuclei, and long interspersed nuclear element 1 (L1) retroelements
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Naïve Bayes classifier assisted automated detection of cerebral microbleeds in susceptibility-weighted imaging brain images. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-08-28 Tayyab Ateeq,Zaid Bin Faheem,Mohamed Ghoneimy,Jehad Ali,Yang Li,Abdullah Baz
Cerebral microbleeds (CMBs) in the brain are the essential indicators of critical brain disorders such as dementia and ischemic stroke. Generally, CMBs are detected manually by experts, which is an exhaustive task with limited productivity. Since CMBs have complex morphological nature, manual detection is prone to errors. This paper presents a machine learning-based automated CMB detection technique
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NDUFA4 promotes the progression of head and neck paraganglioma by inhibiting ferroptosis. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-08-21 Zhigang Wang,Erxing Tao,Yiming Chen,Qi Wang,Min Liu,Liang Wei,Siyi Xu,Wei Chen,Chunlong Zhong
NDUFA4 is a component of respiratory chain-oxidative phosphorylation pathway. NDUFA4 is highly expressed in tumor tissues, but little is known about the function of NDUFA4 in head and neck paraganglioma (HNPGL). We examined NDUFA4 expression in tissues from 10 HNPGL patients and 6 controls using qRT-PCR and Western blotting. NDUFA4 knockdown PGL-626 cells were established by using lentivirus infection
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Automatic detection of Cryptosporidium in optical microscopy images Using YOLOv5x: A comparative study. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-08-16 Johan Sebastian Lopez Salguero,Melissa Rodríguez Rendón,Jessica Triviño Valencia,Jorge Andrés Cuellar Gil,Carlos Andrés Naranjo Galvis,Oscar Moscoso Londoño,César Leandro Londoño Calderón,Fabio Augusto Gonzáles Osorio,Reinel Tabares Soto
Here, a machine learning tool (YOLOv5) enables the detection of Cryptosporidium microorganisms using optical and phase contrast microscope images. The two databases were processed using 520 images (optical microscopy) and 1200 images (phase contrast microscopy). It used Python libraries to label, standardize the size, and crop the images to generate the input tensors to the YOLOv5 network (s, m, and
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LncRNAs: the good, the bad, and the unknown. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-08-14 Ganesan Arunkumar
Long non-coding RNAs (lncRNAs) are significant contributors in maintaining genomic integrity through epigenetic regulation. LncRNAs can interact with chromatin-modifying complexes in both cis and trans pathways, drawing them to specific genomic loci and influencing gene expression via DNA methylation, histone modifications, and chromatin remodeling. They can also operate as building blocks to assemble
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Modulating the immune system as a therapeutic target for myelodysplastic syndromes and acute myeloid leukemia. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-08-11 Caroline M Putnam,Lahari Kondeti,Meredith B A Kesler,Melinda E Varney
Modulating the immune system to treat diseases, including myeloid malignancies, has resulted in the development of a multitude of novel therapeutics in recent years. Myelodysplastic syndromes or neoplasms (MDS) and acute myeloid leukemia (AML) are hematologic malignancies that arise from defects in hematopoietic stem and progenitor cells (HSPCs). Dysregulated immune responses, especially in innate
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Attention-based deep learning framework to recognize diabetes disease from cellular retinal images. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-07-20 Deep Kothadiya,Amjad Rehman,Sidra Abbas,Faten S Alamri,Tanzila Saba
A medical disorder known as diabetic retinopathy (DR) affects people who suffer from diabetes. Many people are visually impaired due to DR. Primary cause of DR in patients is high blood sugar, and it affects blood vessels available in the retinal cell. The recent advancement in deep learning and computer vision methods, and their automation applications can recognize the presence of DR in retinal cells
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The G protein-coupled receptor GPRC5A-a phorbol ester and retinoic acid-induced orphan receptor with roles in cancer, inflammation, and immunity. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-07-19 Pablo A Iglesias González,Ángel G Valdivieso,Tomás A Santa-Coloma
GPRC5A is the first member of a new class of orphan receptors coupled to G proteins, which also includes GPRC5B, GPRC5C, and GPRC5D. Since its cloning and identification in the 1990s, substantial progress has been made in understanding the possible functions of this receptor. GPRC5A has been implicated in a variety of cellular events, such as cytoskeleton reorganization, cell proliferation, cell cycle
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Nootkatone attenuates airway inflammation in asthmatic mice through repressing ROS-induced NLRP3 inflammasome activation. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-07-19 Yun Gai,Chong Bai,Wei Zhang,Hua Xiao,Jing Xu,Jia Hou,Xiahui Ge
Nootkatone (NKT) exhibits potential pharmacological activities including anti-oxidation and anti-inflammation. Nevertheless, little is known about the roles of NKT in asthmatic airway inflammation. In the study, mice were sensitized and challenged with ovalbumin (OVA) to establish experimental allergic asthma model. After treatment with NKT, lung tissues, peripheral blood, and bronchoalveolar lavage
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Comparative analysis of the effect of hypoxia in two different tumor cell models shows the differential involvement of PTEN control of proangiogenic pathways. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-07-17 Aleksandra Majewska,Klaudia Brodaczewska,Aleksandra Filipiak-Duliban,Claudine Kieda
Hypoxia, low, non-physiological oxygen tension is a key regulator of tumor microenvironment, determining the pathological tumor vascularization. Alleviation of hypoxia through vessel normalization may be a promising therapeutic approach. We aimed to assess the role of low oxygen tension in PTEN-related pathways and proangiogenic response, in vitro, in two different tumor cell lines, focusing on potential
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The effects of cetuximab and cisplatin anti-cancer drugs on the mechanical properties of the lung cancerous cells using atomic force microscope. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-07-12 Iraj Rezaei,Ali Sadeghi
Each anti-cancer drug has special effects on the target cells. One of the most important reasons to recommend an anti-cancer drug is related to the influences of it on the mechanical properties of the target cells. In this study, the effects of cetuximab and cisplatin anti-cancer drugs on the mechanical properties of A-549 and Calu-6 cells as the cancerous lung cells have been investigated. For both
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Targeting PTGES/PGE2 axis enhances sensitivity of colorectal cancer cells to 5-fluorouracil. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-06-26 Song Geng,Hao Zhan,Lianmeng Cao,Longlong Geng,Xiang Ren
Insensitivity and resistance to 5-fluorouracil (5FU) remain as major hurdles for effective and durable 5FU-based chemotherapy in colorectal cancer (CRC) patients. In this study, we identified prostaglandin E synthase (PTGES)/prostaglandin E2 (PGE2) axis as an important regulator for 5FU sensitivity in CRC cells. We found that PTGES expression and PGE2 production are elevated in CRC cells in comparison
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HSPA4 regulated glioma progression via activation of AKT signaling pathway. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-06-20 Xi Yuan,Xiangdong Sun,Bin Zhou,Shuang Zhao,Yikun Li,Haolang Ming
Glioma is still an incurable disease with high invasiveness. Heat shock 70 kDa protein 4 (HSPA4) is a member of the HSP110 family, and is associated with the development and progression of various cancers. In the current study, we assessed the expression of HSPA4 in clinical samples, and found that HSPA4 was up-regulated in glioma tissues and correlated with tumor recurrence and grade. Survival analyses
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Remembering the legacy of Professor Mohammad Hashemi: a pioneer in molecular genetic studies in southeast Iran (1965-2019). Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-05-29 Farhad Tabasi,Ebrahim Eskandari,Saeid Ghavami
Professor Mohammad Hashemi was a clinical biochemist and cancer genetic scientist. He has been chair and head of Department of Clinical Biochemistry at Zahedan University of Medical Sciences, Zahedan, Iran. He has played an important role in the improvement of understanding of genetics of disease in southeast Iran. He was also a part of international team for the discovery of the role of calprotectin
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Dysregulation of insulin-like growth factor-1 signaling in postnatal bone elongation. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-05-29 Cassaundra A White,Maria A Serrat
Insulin-like growth factor-1 (IGF-1) is a critical modulator of cell growth and survival, making it a central part of maintaining essentially every biological system in the body. Knowledge of the intricate mechanisms involved in activating IGF-1 signaling is not only key to understanding basic processes of growth and development, but also for addressing diseases, such as cancer and diabetes. This brief
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Suppression of academics and school training in Iran during the "Woman, Life, Freedom" revolution. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-05-19 Shahla Shojaei,Nima Taefehshokr,Saeid Ghavami
For the past 6 months, there has been an ongoing revolution in Iran after the brutal death of Zhina (Mahsa) Amini in morality police custody. Iranian universities' professors and students have been on the frontline of this revolution and have been fired or sentenced. On the other hand, Iranian high schools and primary schools have been under suspected toxic gas attack. In the current article, the latest
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Serum pro-surfactant protein B is correlated with clinical properties in osteosarcoma patients. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-05-16 Shi Feng,Di Fu,Yong Zhang,Le Zhang,Yingnan Ji,Hongqiu Li,Liang A
It is critical to find efficient non-invasive prognostic factor for osteosarcoma. In this study, we demonstrated that serum protein of pro-surfactant protein B (pro-SFTPB) may be a potential diagnostic indicator in osteosarcoma. We found that serum pro-SFTPB was highly expressed in osteosarcoma patients and presented good diagnostic value to discern osteosarcoma patients from non-osteosarcoma control
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Effects of clopidogrel bisulfate on B16-F10 cells and tumor development in a murine model of melanoma. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-05-10 Matheus Henrique Jantsch,Pedro Henrique Doleski,Altevir Rossato Viana,Jean Lucas Gutknecht da Silva,Daniela Ferreira Passos,Fernanda Licker Cabral,Alessandra Guedes Manzoni,Renan da Silva Ebone,Ana Bárbara Uchoa Soares,Cínthia Melazzo de Andrade,Maria Rosa Chitolina Schetinger,Daniela Bitencourt Rosa Leal
Metastatic melanoma is a very aggressive skin cancer. Platelets are constituents of the tumor microenvironment and, when activated, contribute to cancer progression, especially metastasis and inflammation. P2Y12 is an adenosine diphosphate receptor that triggers platelet activation. Inhibition of P2Y12 by clopidogrel bisulfate (CB) decreases platelet activation, which is also controlled by the extracellular
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Histone chaperone SSRP1 is required for apoptosis inhibition and mitochondrial function in HCC via transcriptional promotion of TRAP1. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-04-21 Xuyang Chen,Mengxin Li,Ding Wang,Qian Wang,Xiaodong Wei,Xiaorui Liu,Jiaying Yang,Dhan V Kalvakolanu,Baofeng Guo,Ling Zhang
Epigenetic regulation contributes to human health and disease, especially cancer, but the mechanisms of many epigenetic regulators remain obscure. Most research is focused on gene regulatory processes, such as mRNA translation and DNA damage repair, rather than the effects on biological functions like mitochondrial activity and oxidative phosphorylation. Here, we identified an essential role for the
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Message from Dr. Philippe T. Georgel, Guest Editor for the Marshall University Collection, Brad D. Smith, President of Marshall University, and Dr. Anivandan Mukherjee, Provost of Marshall University. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-04-13 Philippe T Georgel,Brad D Smith,Avinandan Mukherjee
Welcome to the Biochemistry and Cell Biology (BCB) Marshall University Collection. In this series, selected papers authored by Marshall University Faculty and Students are highlighted. The inaugural set of manuscripts is based on research projects presented during the 2022 Marshall University Student research and Creativity Symposium in Huntington, West Virginia (see details below). The entire Marshall
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Evaluation of biochemical profile and oxidative damage to lipids and proteins in patients with lysosomal acid lipase deficiency. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-04-12 Gilian Guerreiro,Marion Deon,Carmen Regla Vargas
Lysosomal acid lipase deficiency (LALD) is an inborn error of metabolism that lacks satisfactory treatment, which leads to the development of severe hepatic and cardiac complications and may even lead to death. In this sense, knowledge of the mechanisms involved in the pathophysiology of this disorder becomes essential to allow the search for new therapeutic strategies. There are no studies in the
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Analysis of financial challenges faced by graduate students in Canada. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-04-11 Sarah Jane Laframboise,Thomas Bailey,Anh-Thu Dang,Mercedes Rose,Zier Zhou,Matthew D Berg,Stephen Holland,Sami Aftab Abdul,Kaela O'Connor,Sara El-Sahli,Dominique M Boucher,Garrett Fairman,Jacky Deng,Katherine Shaw,Nathaniel Noblett,Alexa D'Addario,Madelaine Empey,Keaton Sinclair
Graduate students are vital to the creation of research and innovation in Canada. The National Graduate Student Finance Survey was launched in 2021 by the Ottawa Science Policy Network to investigate the financial realities of Canadian graduate students. Closing in April 2022, the survey received 1305 responses from graduate students representing various geographical locations, years of study, fields
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Mechanism of myocardial fibrosis regulation by IGF-1R in atrial fibrillation through the PI3K/Akt/FoxO3a pathway. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-04-05 Pei Zhang,Huilin Li,An Zhang,Xiao Wang,Qiyuan Song,Zhan Li,Weizong Wang,Jingwen Xu,Yinglong Hou,Yong Zhang
Atrial structural remodeling takes on a critical significance to the occurrence and maintenance of atrial fibrillation (AF). As revealed by recent data, insulin-like growth factor-1 receptor (IGF-1R) plays a certain role in tissue fibrosis. In this study, the mechanism of IGF-1R in atrial structural remodeling was examined based on in vivo and in vitro experiments. First, cluster analysis of AF hub
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AKT1 participates in ferroptosis vulnerability by driving autophagic degradation of FTH1 in cisplatin-resistant ovarian cancer. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-04-03 Zhikun Shi,Hao Yuan,Lanqing Cao,Yang Lin
Resistance to cisplatin (DDP)-based chemotherapy is an important reason for the failure of ovarian cancer treatment. However, tumor cells resistant to chemotherapy may expose vulnerability to other cell death pathways. Here, we found that DDP-resistant ovarian cancer cells are more susceptible to erastin-induced ferroptosis. It should be noted that this vulnerability does not depend on the weakening
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Telomere biology and ribosome biogenesis: structural and functional interconnections. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-03-29 Liia R Valeeva,Liliia R Abdulkina,Inna A Agabekian,Eugene V Shakirov
Telomeres are nucleoprotein structures that play a pivotal role in the protection and maintenance of eukaryotic chromosomes. Telomeres and the enzyme telomerase, which replenishes telomeric DNA lost during replication, are important factors necessary to ensure continued cell proliferation. Cell proliferation is also dependent on proper and efficient protein synthesis, which is carried out by ribosomes
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Astrocyte-specific Ca2+ activity: Mechanisms of action, experimental tools, and roles in ethanol-induced dysfunction. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-03-29 O R Coulter,C D Walker,M-L Risher
Astrocytes are a subtype of non-neuronal glial cells that reside in the central nervous system. Astrocytes have extensive peripheral astrocytic processes that ensheathe synapses to form the tripartite synapse. Through a multitude of pathways, astrocytes can influence synaptic development and structural maturation, respond to neuronal signals, and modulate synaptic transmission. Over the last decade
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Retraction: Role of the TSLP-DC-OX40L pathway in asthma pathogenesis and airway inflammation in mice. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-03-28
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Retraction: Wnt5A regulates the expression of ROR2 tyrosine kinase receptor in ovarian cancer cells. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-03-28
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High concentrations of fructose cause brain damage in mice. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-03-22 Anderson Cargnin-Carvalho,Mariella Reinol da Silva,Ana Beatriz Costa,Nicole Alessandra Engel,Bianca Xavier Farias,Joice Benedet Bressan,Kassiane Mathiola Backes,Francielly de Souza,Naiana da Rosa,Aloir Neri de Oliveira Junior,Mariana Pereira de Souza Goldim,Maria Eduarda Anastácio Borges Correa,Ligia Milanez Venturini,Jucélia Jeremias Fortunato,Josiane Somariva Prophiro,Fabrícia Petronilho,Paulo Cesar
Excessive fructose consumption is associated with the incidence of obesity and systemic inflammation, resulting in increased oxidative damage and failure to the function of brain structures. Thus, we hypothesized that fructose consumption will significantly increase inflammation, oxidative damage, and mitochondrial dysfunction in the mouse brain and, consequently, memory damage. The effects of different
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SARS-CoV-2 E protein-induced THP-1 pyroptosis is reversed by Ruscogenin. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-03-16 Houda Huang,Xiuzhen Li,Duoduo Zha,Hongru Lin,Lingyi Yang,Yihan Wang,Luyan Xu,Linsiqi Wang,Tianhua Lei,Zhou Zhou,Yun-Fei Xiao,Hong-Bo Xin,Mingui Fu,Yisong Qian
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), an emerging pathogenic coronavirus, has been reported to cause excessive inflammation and dysfunction in multiple cells and organs, but the underlying mechanisms remain largely unknown. Here we showed exogenous addition of SARS-CoV-2 envelop protein (E protein) potently induced cell death in cultured cell lines, including THP-1 monocytic
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Retraction: MiR-154 inhibits the growth of laryngeal squamous cell carcinoma by targeting GALNT7. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-03-06
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The nuclear receptor subfamily 4 group A1 in human disease. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-03-02 Hongshuang Wang,Mengjuan Zhang,Fang Fang,Chang Xu,Jiazhi Liu,Lanjun Gao,Chenchen Zhao,Zheng Wang,Yan Zhong,Xiangting Wang
Nuclear receptor 4A1 (NR4A1), a member of the NR4A subfamily, acts as a gene regulator in a wide range of signaling pathways and responses to human diseases. Here, we provide a brief overview of the current functions of NR4A1 in human diseases and the factors involved in its function. A deeper understanding of these mechanisms can potentially improve drug development and disease therapy.
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Hedgehog signal activates AMPK via Smoothened to promote autophagy and lipid degradation in hepatocytes. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-02-23 Yixing Yao,Tianyuan Li,Tingting Yu,Xin Yang,Yue Wang,Jing Cai,Steven Y Cheng,Chen Liu,Shen Yue
Studies in the past decade have shown that lipid droplets stored in liver cells under starvation are encapsulated by autophagosomes and fused to lysosomes via the endocytic system. Autophagy responds to a variety of environmental factors inside and outside the cell, so it has a complex signal regulation network. To this end, we first explored the role of Hedgehog (Hh) in autophagy and lipid metabolism
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Mitogen- and stress-activated protein kinase (MSK1/2) regulated gene expression in normal and disease states. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-02-22 Hedieh Sattarifard,Akram Safaei,Enzhe Khazeeva,Mojgan Rastegar,James R Davie
The mitogen- and stress-activated protein kinases (MSK) are epigenetic modifiers that regulate gene expression in normal and disease cell states. MSK1 and 2 are involved in a chain of signal transduction events bringing signals from the external environment of a cell to specific sites in the genome. MSK1/2 phosphorylate histone H3 at multiple sites, resulting in chromatin remodeling at regulatory elements
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Structural features, intrinsic disorder, and modularity of a pyriform spidroin 1 core repetitive domain. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-02-21 Jeffrey R Simmons,Geneviève Gasmi-Seabrook,Jan K Rainey
Orb-weaving spiders produce up to seven silk types, each with distinct biological roles, protein compositions, and mechanics. Pyriform (or piriform) silk is composed of pyriform spidroin 1 (PySp1) and is the fibrillar component of attachment discs that attach webs to substrates and to each other. Here, we characterize the 234-residue repeat unit (the "Py unit") from the core repetitive domain of Argiope
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ERCC6 plays a promoting role in the progression of non-small cell lung cancer. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-02-21 Hui Luo,Zhehao Xiao,Cheng Huang,Wei Wu,Qingbin Xie
Although excision repair cross-complementing group 6 (ERCC6) has been reported to be associated with lung cancer risk, the specific roles of ERCC6 in non-small cell lung cancer (NSCLC) progression are inadequately studied. Thus, this study aimed to examine the potential functions of ERCC6 in NSCLC. The expression of ERCC6 in NSCLC was analyzed by immunohistochemical staining and quantitative PCR. Celigo
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GCN5 regulates ZBTB16 through acetylation, mediates osteogenic differentiation, and affects orthodontic tooth movement. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-02-14 Shu-Man Shi,Ting-Ting Liu,Xue-Qin Wei,Ge-Hong Sun,Lin Yang,Juan-Fang Zhu
In the process of orthodontic tooth movement (OTM), periodontal ligament fibroblasts (PDLFs) must undergo osteogenic differentiation. OTM increased the expression of Zinc finger and BTB domain-containing 16 (ZBTB16), which is implicated in osteogenic differentiation. Our goal was to investigate the mechanism of PDLF osteogenic differentiation mediated by ZBTB16. The OTM rat model was established, and
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Lipid-induced alteration in retinoic acid signaling leads to mitochondrial dysfunction in HepG2 and Huh7 cells. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-02-14 Eshani Karmakar,Nabanita Das,Bidisha Mukherjee,Prosenjit Das,Satinath Mukhopadhyay,Sib Sankar Roy
A surfeit of mitochondrial reactive oxygen species (ROS) and inflammation serve as obligatory mediators of lipid-associated hepatocellular maladies. While retinoid homeostasis is essential in restoring systemic energy balance, its role in hepatic mitochondrial function remains elusive. The role of lecithin-retinol acyltransferase (LRAT) in maintenance of retinoid homeostasis is appreciated earlier;
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Maternal use of methamphetamine alters cardiovascular function in the adult offspring. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-02-10 Adam M Belcher,Boyd R Rorabaugh
Methamphetamine is one of the most commonly used illicit drugs during pregnancy. Most studies investigating the impact of maternal use of methamphetamine on children have focused on neurological outcomes. In contrast, cardiovascular outcomes in these children have not been characterized. Recent studies in rodents provide evidence that prenatal exposure to methamphetamine induces changes in cardiac
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Emerging roles for heterogeneous ribonuclear proteins in normal and malignant B cells. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-02-06 Qurat Ul Ain Qureshi,Timothy E Audas,Ryan D Morin,Krysta M Coyle
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are among the most abundantly expressed RNA binding proteins in the cell and play major roles in all facets of RNA metabolism. hnRNPs are increasingly appreciated as essential for mammalian B cell development by regulating the carefully ordered expression of specific genes. Due to this tight regulation of the hnRNP-RNA network, it is no surprise that
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Evaluation of drug-free methods for the detection of gene silencing in Saccharomyces cerevisiae. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-01-20 Kholoud Shaban,Safia Mahabub Sauty,Ashley Fisher,Ashley Cheng,Krassimir Yankulov
Multiple studies in Saccharomyces cerevisiae have measured the levels of gene silencing by inserting the URA3 gene at various loci and selecting against URA3-expressing cells by 5-flouroorotic acid (5-FOA). However, 5-FOA affects the cellular pools of dNTPs and can produce side effects. To circumvent this issue, we and others have introduced drug-free techniques to detect silent and active gene states
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Epidermal melanocytes metabolize extracellular nucleotides by purinergic enzymes. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-01-19 Liliana Ivet Sous Naasani,Jéssica Gonçalves Azevedo,Jean Sévigny,Tiago Franco de Oliveira,Silvya Stuchi Maria-Engler,Márcia Rosângela Wink
The human epidermal melanocyte (hEM) are melanin-producing cells that provide skin pigmentation and protection against ultraviolet radiation. Although purinergic signaling is involved in skin biology and pathology, the presence of NTPDase members, as well as the rate of nucleotides degradation by melanocytes were not described yet. Therefore, in this study, we analyzed the expression of ectonucleotidases
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RNA deadenylation complexes in development and diseases. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-01-16 Yilin Liu,Niveditha Ramkumar,Ly P Vu
RNA deadenylation, the process of shortening of the 3' poly(A) tail of an RNA molecule, is one of the key steps of post-transcriptional regulation of gene expression in eukaryotic cells. PAN2/3 and CCR4-NOT (CNOT) are the two dominant RNA deadenylation complexes, which play central roles in mediating mRNA decay and translation. While degradation is the final fate of virtually all RNAs in their life
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Effects of the omega-3 fatty acid DHA on histone and p53 acetylation in diffuse large B cell lymphoma. Biochem. Cell Biol. (IF 2.9) Pub Date : 2023-01-04 Tanner J Bakhshi,Tanner Way,Bradley Muncy,Philippe T Georgel
Diffuse large B cell lymphoma (DLBCL) often develops resistance and/or relapses in response to immunochemotherapy. Epigenetic modifiers are frequently mutated in DLBCL, i.e., the lysine (histone) acetyltransferases CREBBP and EP300. Mutations in CBP/p300 can prevent the proper acetylation and activation of (i) enhancer sequences of genes required for essential functions (e.g., germinal center exit
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HDAC1 overexpression promoted by METTL3-IGF2BP2 inhibits FGF21 expression in metabolic syndrome-related liver injury. Biochem. Cell Biol. (IF 2.9) Pub Date : 2022-12-21 Yunjiang Chen,Kaiyu Cai,Yueling Du,Zixiong Liu,Yanchun Gong
Metabolic syndrome (MetS) represents a cluster of diseases that includes diabetes and insulin resistance. A combination of these metabolic disorders damages liver function. We hypothesized here that histone deacetylase 1 (HDAC1) inhibits fibroblast growth factor 21 (FGF21) expression through histone deacetylation, thereby accentuating liver injury in rats with MetS. MetS rats induced by a high-fat
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MTHFD2 suppresses glioblastoma progression via the inhibition of ERK1/2 phosphorylation. Biochem. Cell Biol. (IF 2.9) Pub Date : 2022-12-09 Meihui Huang,Jiajian Xue,Zhiming Chen,Xiao Zhou,Mantong Chen,Jianhong Sun,Zhennan Xu,Shaohong Wang,Haixiong Xu,Zepeng Du,Mingfa Liu
Glioblastoma (GBM) is a WHO grade 4 tumor and is the most malignant form of glioma. Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2), a mitochondrial enzyme involved in folate metabolism, has been reported to be highly expressed in several human tumors. However, little is known about the role of MTHFD2 in GBM. In this study, we aimed to explore the biological functions of MTHFD2 in GBM and identify