-
The subapical labial sensory organ of spotted lanternfly Lycorma delicatula Open Biol. (IF 5.8) Pub Date : 2024-03-27 Hany K. M. Dweck, Claire E. Rutledge
Deciphering how spotted lanternfly (SLF), an invasive polyphagous planthopper in North America, engages with its environment is a pressing issue with fundamental biological significance and economic importance. This interaction primarily depends on olfaction. However, the cellular basis of olfaction in SLF remains elusive. Here we investigate the neuronal and functional organization of the subapical
-
A nanobody inhibitor of Fascin-1 actin-bundling activity and filopodia formation Open Biol. (IF 5.8) Pub Date : 2024-03-20 Selena G. Burgess, Nikki R. Paul, Mark W. Richards, James R. Ault, Laurie Askenatzis, Sophie G. Claydon, Ryan Corbyn, Laura M. Machesky, Richard Bayliss
Fascin-1-mediated actin-bundling activity is central to the generation of plasma membrane protrusions required for cell migration. Dysregulated formation of cellular protrusions is observed in metastatic cancers, where they are required for increased invasiveness, and is often correlated with increased Fascin-1 abundance. Therefore, there is interest in generating therapeutic Fascin-1 inhibitors. We
-
Interaction of MLE with CLAMP zinc finger is involved in proper MSL proteins binding to chromosomes in Drosophila Open Biol. (IF 5.8) Pub Date : 2024-03-13 Evgeniya Tikhonova, Anastasia Revel-Muroz, Pavel Georgiev, Oksana Maksimenko
The Drosophila male-specific lethal (MSL) complex binds to the male X chromosome to activate transcription. It comprises five proteins (MSL1, MSL2, MSL3, male absent on the first (MOF), and maleless (MLE)) and two long noncoding RNAs (lncRNAs; roX1 and roX2). The MLE helicase remodels the roX lncRNAs, enabling the lncRNA-mediated assembly of the Drosophila dosage compensation complex. MSL2 is expressed
-
The fission yeast NDR kinase Orb6 and its signalling pathway MOR regulate cytoplasmic microtubule organization during the cell cycle Open Biol. (IF 5.8) Pub Date : 2024-03-06 Kazunori Kume, Kenji Nishikawa, Rikuto Furuyama, Takahiro Fujimoto, Takayuki Koyano, Makoto Matsuyama, Masaki Mizunuma, Dai Hirata
Microtubule organization and reorganization during the cell cycle are achieved by regulation of the number, distribution and activity of microtubule-organizing centres (MTOCs). In fission yeast, the Mto1/2 complex determines the activity and distribution of cytoplasmic MTOCs. Upon mitosis, cytoplasmic microtubule nucleation ceases; inactivation of the Mto1/2 complex is triggered by Mto2 hyperphosphorylation
-
Genome editing in East African cichlids and tilapias: state-of-the-art and future directions. Open Biol. (IF 5.8) Pub Date : 2023-11-29 Bethan Clark,Muktai Kuwalekar,Bettina Fischer,Joost Woltering,Jakob Biran,Scott Juntti,Claudius F Kratochwil,M Emília Santos,Miguel Vasconcelos Almeida
African cichlid fishes of the Cichlidae family are a group of teleosts important for aquaculture and research. A thriving research community is particularly interested in the cichlid radiations of the East African Great Lakes. One key goal is to pinpoint genetic variation underlying phenotypic diversification, but the lack of genetic tools has precluded thorough dissection of the genetic basis of relevant
-
Decoding life's inner workings: advances in quantitative bioimaging. Open Biol. (IF 5.8) Pub Date : 2023-11-29 Ricardo Henriques,Christophe Leterrier,Aubrey Weigel
This special feature of Open Biology, titled 'Advances in Quantitative Bioimaging', proposes an overview of the latest advancements in quantitative bioimaging techniques and their wide-ranging applications. The articles cover various topics, including modern imaging methods that enable visualization on a nanoscale, such as super-resolution microscopy and single-particle analysis. These techniques offer
-
RNA binding protein AUF1/HNRNPD regulates nuclear export, stability and translation of SNCA transcripts. Open Biol. (IF 5.8) Pub Date : 2023-11-22 Fedon-Giasin Kattan,Pelagia Koukouraki,Athanasios K Anagnostopoulos,George T Tsangaris,Epaminondas Doxakis
Alpha-synuclein (SNCA) accumulation plays a central role in the pathogenesis of Parkinson's disease. Determining and interfering with the mechanisms that control SNCA expression is one approach to limiting disease progression. Currently, most of our understanding of SNCA regulation is protein-based. Post-transcriptional mechanisms directly regulating SNCA mRNA expression via its 3' untranslated region
-
Coiled-coil-mediated dimerization of Atg16 is required for binding to the PROPPIN Atg21. Open Biol. (IF 5.8) Pub Date : 2023-11-22 Miranda Bueno-Arribas,Celia Cruz-Cuevas,María-Angeles Navas,Ricardo Escalante,Olivier Vincent
PROPPINs/WIPIs are β-propeller proteins that bind phosphoinositides and contribute to the recruitment of protein complexes involved in membrane remodelling processes such as autophagosome formation and endosomal trafficking. Yeast Atg21 and mammalian WIPI2 interact with Atg16/ATG16L1 to mediate recruitment of the lipidation machinery to the autophagosomal membrane. Here, we used the reverse double
-
Cellular and molecular signatures of motherhood in the adult and ageing rat brain. Open Biol. (IF 5.8) Pub Date : 2023-11-22 P Duarte-Guterman,J E Richard,S E Lieblich,R S Eid,Y Lamers,L A M Galea
Pregnancy is marked by robust changes, including brain changes to volume, structure, connectivity and neuroplasticity. Although some brain changes are restricted to pregnancy and the postpartum, others are long-lasting. Few studies have examined possible mechanisms of these changes or the effects of multiple pregnancies. We characterized various cellular and molecular signatures of parity (nulliparous
-
Insulin receptor Arg717 and IGF-1 receptor Arg704 play a key role in ligand binding and in receptor activation. Open Biol. (IF 5.8) Pub Date : 2023-11-08 Anna Kertisová,Lenka Žáková,Kateřina Macháčková,Aleš Marek,Pavel Šácha,Petr Pompach,Jiří Jiráček,Irena Selicharová
The insulin receptor (IR, with its isoforms IR-A and IR-B) and the insulin-like growth factor 1 receptor (IGF-1R) are related tyrosine kinase receptors. Recently, the portfolio of solved hormone-receptor structures has grown extensively thanks to advancements in cryo-electron microscopy. However, the dynamics of how these receptors transition between their inactive and active state are yet to be fully
-
Multi-epitope vaccine candidates based on mycobacterial membrane protein large (MmpL) proteins against Mycobacterium ulcerans. Open Biol. (IF 5.8) Pub Date : 2023-11-08 Tamara Z Ishwarlall,Victoria T Adeleke,Leah Maharaj,Moses Okpeku,Adebayo A Adeniyi,Matthew A Adeleke
Buruli ulcer (BU) is a neglected tropical disease. It is caused by the bacterium Mycobacterium ulcerans and is characterized by skin lesions. Several studies were performed testing the Bacillus Calmette-Guérin (BCG) vaccine in human and animal models and M. ulcerans-specific vaccines in animal models. However, there are currently no clinically accepted vaccines to prevent M. ulcerans infection. The
-
Differential modulation of innate immune response by lipopolysaccharide of Leptospira. Open Biol. (IF 5.8) Pub Date : 2023-11-08 Vivek P Varma,Ramudu Bankala,Ajay Kumar,Shashikant Gawai,Syed M Faisal
Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira spp. having more than 300 serovars. These serovars can infect a variety of hosts, some being asymptomatic carriers and others showing varied symptoms of mild to severe infection. Since lipopolysaccharide (LPS) is the major antigen which defines serovar specificity, this different course of infection may be attributed to a differential
-
Pericentric major satellite transcription is essential for meiotic chromosome stability and spindle pole organization. Open Biol. (IF 5.8) Pub Date : 2023-11-08 Claudia Baumann,Xiangyu Zhang,Maria M Viveiros,Rabindranath De La Fuente
In somatic cells, mitotic transcription of major satellite non-coding RNAs is tightly regulated and essential for heterochromatin formation and the maintenance of genome integrity. We recently demonstrated that major satellite transcripts are expressed, and chromatin-bound during mouse oocyte meiosis. Pericentric satellite RNAs are also expressed in human oocytes. However, the specific biological function(s)
-
Structure and molecular basis of spermatid elongation in the Drosophila testis. Open Biol. (IF 5.8) Pub Date : 2023-11-08 Qiuru Huang,Xia Chen,Hao Yu,Li Ji,Yi Shi,Xinmeng Cheng,Hao Chen,Jun Yu
Spermatid elongation is a crucial event in the late stage of spermatogenesis in the Drosophila testis, eventually leading to the formation of mature sperm after meiosis. During spermatogenesis, significant structural and morphological changes take place in a cluster of post-meiotic germ cells, which are enclosed in a microenvironment surrounded by somatic cyst cells. Microtubule-based axoneme assembly
-
Regulation of the heterochromatin spreading reaction by trans-acting factors. Open Biol. (IF 5.8) Pub Date : 2023-11-08 Bulut Hamali,Ahmed A A Amine,Bassem Al-Sady
Heterochromatin is a gene-repressive protein-nucleic acid ultrastructure that is initially nucleated by DNA sequences. However, following nucleation, heterochromatin can then propagate along the chromatin template in a sequence-independent manner in a reaction termed spreading. At the heart of this process are enzymes that deposit chemical information on chromatin, which attracts the factors that execute
-
-
Retraction: 'MALAT1 promotes gastric adenocarcinoma through the MALAT1/miR-181a-5p/AKT3 axis' (2019), by Lu et al. Open Biol. (IF 5.8) Pub Date : 2023-11-03
-
-
-
-
-
Cx43 can form functional channels at the nuclear envelope and modulate gene expression in cardiac cells. Open Biol. (IF 5.8) Pub Date : 2023-11-01 Tania Martins-Marques,Katja Witschas,Ilda Ribeiro,Mónica Zuzarte,Steve Catarino,Teresa Ribeiro-Rodrigues,Francisco Caramelo,Trond Aasen,Isabel Marques Carreira,Lino Goncalves,Luc Leybaert,Henrique Girao
Classically associated with gap junction-mediated intercellular communication, connexin43 (Cx43) is increasingly recognized to possess non-canonical biological functions, including gene expression regulation. However, the mechanisms governing the localization and role played by Cx43 in the nucleus, namely in transcription modulation, remain unknown. Using comprehensive and complementary approaches
-
Formyl-peptide receptor 2 signalling triggers aerobic metabolism of glucose through Nox2-dependent modulation of pyruvate dehydrogenase activity. Open Biol. (IF 5.8) Pub Date : 2023-10-25 Tiziana Pecchillo Cimmino,Ester Pagano,Mariano Stornaiuolo,Gabriella Esposito,Rosario Ammendola,Fabio Cattaneo
The human formyl-peptide receptor 2 (FPR2) is activated by an array of ligands. By phospho-proteomic analysis we proved that FPR2 stimulation induces redox-regulated phosphorylation of many proteins involved in cellular metabolic processes. In this study, we investigated metabolic pathways activated in FPR2-stimulated CaLu-6 cells. The results showed an increased concentration of metabolites involved
-
Examination of gammarid transcriptomes reveals a widespread occurrence of key metabolic genes from epibiont bdelloid rotifers in freshwater species. Open Biol. (IF 5.8) Pub Date : 2023-10-25 Alberto Ribes-Navarro,Naoki Kabeya,L Filipe C Castro,André Gomes-Dos-Santos,Miguel M Fonseca,Hilke Alberts-Hubatsch,Francisco Hontoria,Juan C Navarro,Óscar Monroig
Previous data revealed the unexpected presence of genes encoding for long-chain polyunsaturated fatty acid (LC-PUFA) biosynthetic enzymes in transcriptomes from freshwater gammarids but not in marine species, even though closely related species were compared. This study aimed to clarify the origin and occurrence of selected LC-PUFA biosynthesis gene markers across all published gammarid transcriptomes
-
Temperature activated transient receptor potential ion channels from Antarctic fishes. Open Biol. (IF 5.8) Pub Date : 2023-10-18 Julia M York
Antarctic notothenioid fishes (cryonotothenioids) live in waters that range between -1.86°C and an extreme maximum +4°C. Evidence suggests these fish sense temperature peripherally, but the molecular mechanism of temperature sensation in unknown. Previous work identified transient receptor potential (TRP) channels TRPA1b, TRPM4 and TRPV1a as the top candidates for temperature sensors. Here, cryonotothenioid
-
Genome-wide identification of ATP-binding cassette transporter B subfamily, focusing on its structure, evolution and rearrangement in ciliates. Open Biol. (IF 5.8) Pub Date : 2023-10-04 Xue Zhang,Yan Zhao,Weibo Zheng,Bei Nan,Jinyu Fu,Yu Qiao,Rebecca A Zufall,Feng Gao,Ying Yan
ATP-binding cassette subfamily B (ABCB) has been implicated in various essential functions such as multidrug resistance, auxin transport and heavy metal tolerance in animals and plants. However, the functions, the genomic distribution and the evolutionary history have not been characterized systematically in lower eukaryotes. As a lineage of highly specialized unicellular eukaryotes, ciliates have
-
Gene duplication is the primary driver of intraspecific genomic divergence in coral algal symbionts. Open Biol. (IF 5.8) Pub Date : 2023-09-27 Sarah Shah,Katherine E Dougan,Yibi Chen,Debashish Bhattacharya,Cheong Xin Chan
Dinoflagellates in the order Suessiales include the family Symbiodiniaceae, which have essential roles as photosymbionts in corals, and their cold-adapted sister group, Polarella glacialis. These diverse taxa exhibit extensive genomic divergence, although their genomes are relatively small (haploid size < 3 Gbp) when compared with most other free-living dinoflagellates. Different strains of Symbiodiniaceae
-
The signalling lipid PI3,5P2 is essential for timely mitotic exit. Open Biol. (IF 5.8) Pub Date : 2023-09-27 Mariam Huda,Seyma Nur Bektas,Baris Bekdas,Ayse Koca Caydasi
Coordination of mitotic exit with chromosome segregation is key for successful mitosis. Mitotic exit in budding yeast is executed by the mitotic exit network (MEN), which is negatively regulated by the spindle position checkpoint (SPOC). SPOC kinase Kin4 is crucial for SPOC activation in response to spindle positioning defects. Here, we report that the lysosomal signalling lipid phosphatidylinositol-3
-
Nucleotide-free structures of KIF20A illuminate atypical mechanochemistry in this kinesin-6. Open Biol. (IF 5.8) Pub Date : 2023-09-20 Fanomezana Moutse Ranaivoson,Vincent Crozet,Matthieu P M H Benoit,Amna Abdalla Mohammed Khalid,Carlos Kikuti,Helena Sirkia,Ahmed El Marjou,Stéphanie Miserey-Lenkei,Ana B Asenjo,Hernando Sosa,Christoph F Schmidt,Steven S Rosenfeld,Anne Houdusse
KIF20A is a critical kinesin for cell division and a promising anti-cancer drug target. The mechanisms underlying its cellular roles remain elusive. Interestingly, unusual coupling between the nucleotide- and microtubule-binding sites of this kinesin-6 has been reported, but little is known about how its divergent sequence leads to atypical motility properties. We present here the first high-resolution
-
Migration and differentiation of muscle stem cells are coupled by RhoA signalling during regeneration. Open Biol. (IF 5.8) Pub Date : 2023-09-20 Mirco Brondolin,Dylan Herzog,Sami Sultan,Fiona Warburton,Alessandra Vigilante,Robert D Knight
Skeletal muscle is highly regenerative and is mediated by a population of migratory adult muscle stem cells (muSCs). Effective muscle regeneration requires a spatio-temporally regulated response of the muSC population to generate sufficient muscle progenitor cells that then differentiate at the appropriate time. The relationship between muSC migration and cell fate is poorly understood and it is not
-
Plum modulates Myoglianin and regulates synaptic function in D. melanogaster. Open Biol. (IF 5.8) Pub Date : 2023-09-13 Virender K Sahota,Aelfwin Stone,Nathaniel S Woodling,Jereme G Spiers,Joern R Steinert,Linda Partridge,Hrvoje Augustin
Alterations in the neuromuscular system underlie several neuromuscular diseases and play critical roles in the development of sarcopenia, the age-related loss of muscle mass and function. Mammalian Myostatin (MST) and GDF11, members of the TGF-β superfamily of growth factors, are powerful regulators of muscle size in both model organisms and humans. Myoglianin (MYO), the Drosophila homologue of MST
-
-
Uncoupled redox stress: how a temporal misalignment of redox-regulated processes and circadian rhythmicity exacerbates the stressed state. Open Biol. (IF 5.8) Pub Date : 2023-09-06 Anna D Clark,Andrew F Cumpstey,Jérôme Santolini,Alan A Jackson,Martin Feelisch
Diurnal and seasonal rhythmicity, entrained by environmental and nutritional cues, is a vital part of all life on Earth operating at every level of organization; from individual cells, to multicellular organisms, whole ecosystems and societies. Redox processes are intrinsic to physiological function and circadian regulation, but how they are integrated with other regulatory processes at the whole-body
-
-
-
-
-
-
-
A unifying mechanism for protein transport through the core bacterial Sec machinery. Open Biol. (IF 5.8) Pub Date : 2023-08-30 William J Allen,Ian Collinson
Encapsulation and compartmentalization are fundamental to the evolution of cellular life, but they also pose a challenge: how to partition the molecules that perform biological functions-the proteins-across impermeable barriers into sub-cellular organelles, and to the outside. The solution lies in the evolution of specialized machines, translocons, found in every biological membrane, which act both
-
RNF4 and USP7 cooperate in ubiquitin-regulated steps of DNA replication. Open Biol. (IF 5.8) Pub Date : 2023-08-23 Ya-Chu Chang,Kevin Lin,Ryan M Baxley,Wesley Durrett,Liangjun Wang,Olivera Stojkova,Maximilian Billmann,Henry Ward,Chad L Myers,Anja-Katrin Bielinsky
DNA replication requires precise regulation achieved through post-translational modifications, including ubiquitination and SUMOylation. These modifications are linked by the SUMO-targeted E3 ubiquitin ligases (STUbLs). Ring finger protein 4 (RNF4), one of only two mammalian STUbLs, participates in double-strand break repair and resolving DNA-protein cross-links. However, its role in DNA replication
-
The metabolic control of DNA replication: mechanism and function. Open Biol. (IF 5.8) Pub Date : 2023-08-16 Panos Soultanas,Laurent Janniere
Metabolism and DNA replication are the two most fundamental biological functions in life. The catabolic branch of metabolism breaks down nutrients to produce energy and precursors used by the anabolic branch of metabolism to synthesize macromolecules. DNA replication consumes energy and precursors for faithfully copying genomes, propagating the genetic material from generation to generation. We have
-
Development and repair of blood vessels in the zebrafish spinal cord. Open Biol. (IF 5.8) Pub Date : 2023-08-09 Ana Ribeiro,Mariana Rebocho da Costa,Carmen de Sena-Tomás,Elsa Charas Rodrigues,Raquel Quitéria,Tiago Maçarico,Susana Constantino Rosa Santos,Leonor Saúde
The vascular system is inefficiently repaired after spinal cord injury (SCI) in mammals, resulting in secondary tissue damage and immune deregulation that contribute to the limited functional recovery. Unlike mammals, zebrafish can repair the spinal cord (SC) and restore motility, but the vascular response to injury has not been investigated. Here, we describe the zebrafish SC blood vasculature, starting
-
Genomic mining and diversity of assembly line polyketide synthases. Open Biol. (IF 5.8) Pub Date : 2023-08-02 Shreya Kishore,Chaitan Khosla
Assembly line polyketide synthases (PKSs) are a large family of multifunctional enzymes responsible for synthesizing many medicinally relevant natural products with remarkable structural variety and biological activity. The decrease in cost of genomic sequencing paired with development of computational tools like antiSMASH presents an opportunity to survey the vast diversity of assembly line PKS. Mining
-
Min oscillations in bacteria as real-time reporter of environmental challenges at the single-cell level. Open Biol. (IF 5.8) Pub Date : 2023-07-26 Ingrid V Ortega,Felipe Viela,Cristina Flors
Min oscillations are a fascinating mechanism used by Escherichia coli to find their middle. Beyond their biological role, they provide a convenient and relatively unexplored method to monitor the effect of sublethal environmental challenges on bacterial physiology in real-time and at the single-cell level. In this review, we discuss the original papers that put forward the idea of using Min oscillations
-
Identification of PP2A-B55 targets uncovers regulation of emerin during nuclear envelope reassembly in Drosophila. Open Biol. (IF 5.8) Pub Date : 2023-07-19 Virginie Emond-Fraser,Myreille Larouche,Peter Kubiniok,Éric Bonneil,Jingjing Li,Mohammed Bourouh,Laura Frizzi,Pierre Thibault,Vincent Archambault
Mitotic exit requires the dephosphorylation of many proteins whose phosphorylation was needed for mitosis. Protein phosphatase 2A with its B55 regulatory subunit (PP2A-B55) promotes this transition. However, the events and substrates that it regulates are incompletely understood. We used proteomic approaches in Drosophila to identify proteins that interact with and are dephosphorylated by PP2A-B55
-
Evolution and assembly of Anopheles aquasalis's immune genes: primary malaria vector of coastal Central and South America and the Caribbean Islands. Open Biol. (IF 5.8) Pub Date : 2023-07-12 Cesar Camilo Prado Sepulveda,Rodrigo Maciel Alencar,Rosa Amélia Santana,Igor Belém de Souza,Gigliola Mayra Ayres D'Elia,Raquel Soares Maia Godoy,Ana Paula Duarte,Stefanie Costa Pinto Lopes,Marcus Vinicius Guimarães de Lacerda,Wuelton Marcelo Monteiro,Rafael Nacif-Pimenta,Nágila Francinete Costa Secundino,Leonardo Barbosa Koerich,Paulo Filemon Paolucci Pimenta
Anophelines are vectors of malaria, the deadliest disease worldwide transmitted by mosquitoes. The availability of genomic data from various Anopheles species allowed evolutionary comparisons of the immune response genes in search of alternative vector control of the malarial parasites. Now, with the Anopheles aquasalis genome, it was possible to obtain more information about the evolution of the immune
-
Mutant SPART causes defects in mitochondrial protein import and bioenergetics reversed by Coenzyme Q. Open Biol. (IF 5.8) Pub Date : 2023-07-12 Chiara Diquigiovanni,Nicola Rizzardi,Antje Kampmeier,Irene Liparulo,Francesca Bianco,Bianca De Nicolo,Erica Cataldi-Stagetti,Elisabetta Cuna,Giulia Severi,Marco Seri,Miriam Bertrand,Tobias B Haack,Adela Della Marina,Frederik Braun,Romana Fato,Alma Kuechler,Christian Bergamini,Elena Bonora
Pathogenic variants in SPART cause Troyer syndrome, characterized by lower extremity spasticity and weakness, short stature and cognitive impairment, and a severe mitochondrial impairment. Herein, we report the identification of a role of Spartin in nuclear-encoded mitochondrial proteins. SPART biallelic missense variants were detected in a 5-year-old boy with short stature, developmental delay and
-
A pro-BMP function exerted by Rhodnius prolixus short gastrulation reveals great diversity in the role of BMP modulators during embryonic patterning. Open Biol. (IF 5.8) Pub Date : 2023-07-05 M Berni,J Mota,D Bressan,L Ribeiro,G Martins,J Pereira,I Ramos,R Nunes-da-Fonseca,H Araujo
Dorsal-ventral (DV) patterning is regulated by the bone morphogenetic pathway (BMP) in Bilateria. In insect DV patterning, the Toll pathway also plays a role, in addition to BMPs. Variations in the relative importance of each pathway for DV patterning have been reported using single species of coleopteran, hymenopteran, hemipteran and orthopteran insects. To investigate if the molecular control of
-
Planar cell polarity: intracellular asymmetry and supracellular gradients of Frizzled. Open Biol. (IF 5.8) Pub Date : 2023-06-14 José Casal,Freya Storer,Peter A Lawrence
Planar cell polarity (PCP), the coordinated orientation of structures such as cilia, mammalian hairs or insect bristles, depends on at least two molecular systems. We have argued that these two systems use similar mechanisms; each depending on a supracellular gradient of concentration that spans a field of cells. In a linked paper, we studied the Dachsous/Fat system. We found a graded distribution
-
A new view of axon growth and guidance grounded in the stochastic dynamics of actin networks. Open Biol. (IF 5.8) Pub Date : 2023-06-07 Rameen Forghani,Aravind Chandrasekaran,Garegin Papoian,Edward Giniger
The mechanism of axon growth and guidance is a core, unsolved problem in neuroscience and cell biology. For nearly three decades, our view of this process has largely been based on deterministic models of motility derived from studies of neurons cultured in vitro on rigid substrates. Here, we suggest a fundamentally different, inherently probabilistic model of axon growth, one that is grounded in the
-
Structural insights into a regulatory mechanism of FIR RRM1-FUSE interaction. Open Biol. (IF 5.8) Pub Date : 2023-05-31 Xiaomin Ni,Andreas C Joerger,Apirat Chaikuad,Stefan Knapp
FUBP-interacting repressor (FIR) is a suppressor of transcription of the proto-oncogene MYC. FIR binds to the far upstream element (FUSE) of the MYC promoter. Competition of FIR with FUSE-binding protein 1 (FUBP1) is a key mechanism of MYC transcriptional regulation. To gain insights into the structural mechanisms regulating FIR DNA interaction, we determined the crystal structure of two FIR RRM domains
-
Krüppel-homologue 1 regulates the development of Tuta absoluta and its cascade regulation pattern in the juvenile hormone signalling pathway. Open Biol. (IF 5.8) Pub Date : 2023-05-31 Xiaodi Wang,Siyan Bi,Yanhong Tang,Guifen Zhang,Cong Huang,Fanghao Wan,Zhichuang Lü,Wanxue Liu
Tomato leaf miner, Tuta absoluta (Meyrick), is one of the most destructive quarantine pests globally. It has been confirmed that Krüppel-homologue 1 (kr-h1) plays a key role in the regulation of juvenile hormone (JH). However, it is unclear how kr-h1 regulates the synthesis of JH and its cascade regulation pattern in tomato leaf miner. Here, we obtained the six JH signalling genes (kr-h1, Methoprene-tolerant
-
7-Nitroindazole reduces L-DOPA-induced dyskinesias in non-human Parkinsonian primate. Open Biol. (IF 5.8) Pub Date : 2023-05-17 M T Herrero,J E Yuste,L Cuenca-Bermejo,P Almela,L Arenas-Betancur,V De Pablos,A Gonzalez-Cuello,E Del Bel,J Navarro-Zaragoza,E Fernández-Villalba
Nitric oxide (NO) plays a pivotal role in integrating dopamine transmission in the basal ganglia and has been implicated in the pathogenesis of Parkinson disease (PD). The objective of this study was to ascertain whether the NO synthase inhibitor, 7-nitroindazole (7-NI), is able to reduce L-DOPA-induced dyskinesias (LIDs) in a non-human primate model of PD chronically intoxicated with 1-methyl-4-phenyl-1
-
Caprin-1 binding to the critical stress granule protein G3BP1 is influenced by pH. Open Biol. (IF 5.8) Pub Date : 2023-05-10 Tim Schulte,Marc D Panas,Xiao Han,Lucy Williams,Nancy Kedersha,Jonas Simon Fleck,Timothy J C Tan,Xaquin Castro Dopico,Anders Olsson,Ainhoa Moliner Morro,Leo Hanke,Johan Nilvebrant,Kim Anh Giang,Per-Åke Nygren,Paul Anderson,Adnane Achour,Gerald M McInerney
G3BP is the central node within stress-induced protein-RNA interaction networks known as stress granules (SGs). The SG-associated proteins Caprin-1 and USP10 bind mutually exclusively to the NTF2 domain of G3BP1, promoting and inhibiting SG formation, respectively. Herein, we present the crystal structure of G3BP1-NTF2 in complex with a Caprin-1-derived short linear motif (SLiM). Caprin-1 interacts
-
Quantitative single molecule analysis of podoplanin clustering in fibroblastic reticular cells uncovers CD44 function. Open Biol. (IF 5.8) Pub Date : 2023-05-10 Shu En Lim,Megan D Joseph,Charlotte M de Winde,Sophie E Acton,Sabrina Simoncelli
Upon initial immune challenge, dendritic cells (DCs) migrate to lymph nodes and interact with fibroblastic reticular cells (FRCs) via C-type lectin-like receptor 2 (CLEC-2). CLEC-2 binds to the membrane glycoprotein podoplanin (PDPN) on FRCs, inhibiting actomyosin contractility through the FRC network and permitting lymph node expansion. The hyaluronic acid receptor CD44 is known to be required for
-
Delay eyeblink conditioning performance and brain-wide c-Fos expression in male and female mice. Open Biol. (IF 5.8) Pub Date : 2023-05-10 Maria Roa Oyaga,Ines Serra,Devika Kurup,Sebastiaan K E Koekkoek,Aleksandra Badura
Delay eyeblink conditioning has been extensively used to study associative learning and the cerebellar circuits underlying this task have been largely identified. However, there is a little knowledge on how factors such as strain, sex and innate behaviour influence performance during this type of learning. In this study, we used male and female mice of C57BL/6J (B6) and B6CBAF1 strains to investigate
-
Low-nutrient diet in Drosophila larvae stage causes enhancement in dopamine modulation in adult brain due epigenetic imprinting. Open Biol. (IF 5.8) Pub Date : 2023-05-10 J M Zúñiga-Hernández,Gonzalo H Olivares,Patricio Olguín,Alvaro Glavic
Nutrient scarcity is a frequent adverse condition that organisms face during their development. This condition may lead to long-lasting effects on the metabolism and behaviour of adults due to developmental epigenetic modifications. Here, we show that reducing nutrient availability during larval development affects adult spontaneous activity and sleep behaviour, together with changes in gene expression
-
Opium alkaloids, biosynthesis, pharmacology and association with cancer occurrence. Open Biol. (IF 5.8) Pub Date : 2023-05-03 Agrataben Vadhel,Sabreen Bashir,Ashiq Hussain Mir,Madhuri Girdhar,Deepak Kumar,Anil Kumar,Aradhana Mohan,Tabarak Malik,Anand Mohan
Papaver somniferum L. (Family: Papaveraceae) is a species well known for its diverse alkaloids (100 different benzylisoquinoline alkaloids (BIAs)). L-tyrosine serves as a precursor of several specific metabolites like BIAs. It has been used as an antitussive and potent analgesic to alleviate mild to extreme pain since ancient times. The extraction of pharmaceutically important alkaloids like morphine
-
PK1 from Drosophila obscurin is an inactive pseudokinase with scaffolding properties. Open Biol. (IF 5.8) Pub Date : 2023-04-26 Thomas Zacharchenko,Till Dorendorf,Nicolas Locker,Evert Van Dijk,Anja Katzemich,Kay Diederichs,Belinda Bullard,Olga Mayans
Obscurins are large filamentous proteins with crucial roles in the assembly, stability and regulation of muscle. Characteristic of these proteins is a tandem of two C-terminal kinase domains, PK1 and PK2, that are separated by a long intrinsically disordered sequence. The significance of this conserved domain arrangement is unknown. Our study of PK1 from Drosophila obscurin shows that this is a pseudokinase