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Kallikrein-related peptidase's significance in Alzheimer's disease pathogenesis: A comprehensive survey Biochimie (IF 3.9) Pub Date : 2024-04-10 Rilès Boumali, Laureline Urli, Meriem Naim, Feryel Soualmia, Kiyoka Kinugawa, Isabelle Petropoulos, Chahrazade El Amri
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NADPH oxidase-generated reactive oxygen species are involved in estradiol 17ß-d-glucuronide-induced cholestasis Biochimie (IF 3.9) Pub Date : 2024-04-10 Gimena Salas, Alen A. Litta, Anabela C. Medeot, Virginia S. Schuck, Romina B. Andermatten, Gisel S. Miszczuk, Nadia Ciriaci, Ma Valeria Razori, Ismael R. Barosso, Enrique J. Sánchez Pozzi, Marcelo G. Roma, Cecilia L. Basiglio, Fernando A. Crocenzi
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Galactokinase-like protein from Leishmania donovani: Biochemical and structural characterization of a recombinant protein Biochimie (IF 3.9) Pub Date : 2024-04-04 Hasana Baber, Arega Aghajani, B. Harold Gallimore, Cassandra Bethel, James G. Hyatt, Elizabeth F.B. King, Helen P. Price, Marissa L. Maciej-Hulme, Suat Sari, Anja Winter
Leishmaniasis is a spectrum of conditions caused by infection with the protozoan spp. parasites. Leishmaniasis is endemic in 98 countries around the world, and resistance to current anti-leishmanial drugs is rising. Our work has identified and characterised a previously unstudied galactokinase-like protein (GalK) in , which catalyses the MgATP-dependent phosphorylation of the C-1 hydroxyl group of
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Multifunctional host-defense peptides isolated from skin secretions of the banana tree dwelling frog Boana platanera (Hylidae; Hylinae) Biochimie (IF 3.9) Pub Date : 2024-03-30 J. Michael Conlon, Ananyaa Sridhar, Dawood Khan, Taylor S. Cunning, Jack J. Delaney, Megan G. Taggart, Nigel G. Ternan, Jérôme Leprince, Laurent Coquet, Thierry Jouenne, Samir Attoub, Milena Mechkarska
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Cathepsin D inhibition during neuronal differentiation selectively affects individual proteins instead of overall protein turnover Biochimie (IF 3.9) Pub Date : 2024-03-28 Johannes Schneider, Julia Mitschke, Mahima Bhat, Daniel Vogele, Oliver Schilling, Thomas Reinheckel, Lisa Heß
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The impact of chemotherapy on adipose tissue remodeling: The molecular players involved in this tissue wasting Biochimie (IF 3.9) Pub Date : 2024-03-26 Samuel Barbosa, Mafalda Barbosa Pedrosa, Rita Ferreira, Daniel Moreira-Gonçalves, Lúcio Lara Santos
The depletion of visceral and subcutaneous adipose tissue (AT) during chemotherapy significantly correlates with diminished overall survival and progression-free survival. Despite its clinical significance, the intricate molecular mechanisms governing this AT loss and its chemotherapy-triggered initiation remain poorly understood. Notably, the evaluation of AT remodeling in most clinical trials has
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Inhibition of soluble epoxide hydrolase as a therapeutic approach for blood-brain barrier dysfunction Biochimie (IF 3.9) Pub Date : 2024-03-24 Shuo Li, Huijia Song, Yanping Sun, Yongjun Sun, Huimin Zhang, Zibin Gao
The blood-brain barrier (BBB) is a protective semi-permeable structure that regulates the exchange of biomolecules between the peripheral blood and the central nervous system (CNS). Due to its specialized tight junctions and low vesicle trafficking, the BBB strictly limits the paracellular passage and transcellular transport of molecules to maintain the physiological condition of brain tissues. BBB
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Thrombin stories in the gut Biochimie (IF 3.9) Pub Date : 2024-03-22 Nathalie Vergnolle
Many studies have demonstrated the involvement of proteases in gut physiology and pathophysiology over the recent years. Among them, thrombin has appeared for a long time as an old player only involved in blood clotting upon tissue injury. The fact that thrombin receptors (Protease-Activated Receptors-1 and -4) are expressed and functional in almost all cell types of the gut, contributing to barrier
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Matrix metalloproteinase landscape in the imiquimod-induced skin inflammation mouse model Biochimie (IF 3.9) Pub Date : 2024-03-19 Heidi Kyung Noddeland, Vahap Canbay, Marianne Lind, Simonas Savickas, Louise Bastholm Jensen, Karsten Petersson, Martin Malmsten, Janne Koch, Ulrich auf dem Keller, Andrea Heinz
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Melatonin facts: Melatonin lacks immuno-inflammation boosting capacities at the molecular and cellular levels Biochimie (IF 3.9) Pub Date : 2024-03-18 Jean A. Boutin, Valérie Hamon de Almeida, Nathalie Coussay, Céline Legros, Gilles Ferry, Karine Reybier
Among the properties melatonin is claimed to possess, are the immuno-inflammation inductive capacities that would be responsible of some of the paramount of activities melatonin is reported to have in most of the human pathological conditions. In the present paper, we measured the effect of melatonin on established cellular models of immuno-inflammation, and found none. The discrepancies are discussed
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The mutual and dynamic role of TSPO and ligands in their binding process: An example with PK-11195 Biochimie (IF 3.9) Pub Date : 2024-03-16 Rajas M. Rao, Ibaa El Dhaybi, Frédéric Cadet, Catherine Etchebest, Julien Diharce
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The multifaceted role of proteases and modern analytical methods for investigation of their catalytic activity Biochimie (IF 3.9) Pub Date : 2024-03-15 Tatiana A. Filippova, Rami A. Masamrekh, Yulia Yu. Khudoklinova, Victoria V. Shumyantseva, Alexey V. Kuzikov
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Short-term exposure to cigarette smoke upregulates cathepsin S and alters expression of tight junction ZO-1 Biochimie (IF 3.9) Pub Date : 2024-03-15 Florent Estur, Emilie Murigneux, Alexis David, Mélia Magnen, Ahlame Saidi, Gilles Lalmanach, Fabien Lecaille
A long-term exposure to cigarette smoke (CS) alters the integrity of airway epithelial barrier, contributes to lung dysfunction, and elicits the expression and activity of lung cathepsin S (CatS), a cysteine protease that participates in the remodeling of connective tissue and cell junctions. Here, we observed that a short-term (4 days) exposure of mice to CS increased the expression and activity of
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Unraveling the impact of the p.R107L mutation on the structure and function of human αB-Crystallin: Implications for cataract formation Biochimie (IF 3.9) Pub Date : 2024-03-15 Farid Nasiri, Parisa Ebrahimi, Mohammad Bagher Shahsavani, Anis Barati, Issa Zarei, Jun Hong, Masaru Hoshino, Ali Akbar Moosavi-Movahedi, Reza Yousefi
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The molecular crosstalk of the hippo cascade in breast cancer: A potential central susceptibility Biochimie (IF 3.9) Pub Date : 2024-03-15 Sulfath Thottungal Parambil, Gisha Rose Antony, Ajeesh Babu Littleflower, Lakshmi Subhadradevi
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N-terminal processing by dipeptidyl peptidase 9: Cut and Go! Biochimie (IF 3.9) Pub Date : 2024-03-09 Samuel Zolg, Laura Donzelli, Ruth Geiss-Friedlander
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Exploring ligand interactions with human phosphomannomutases using recombinant bacterial thermal shift assay and biochemical validation Biochimie (IF 3.9) Pub Date : 2024-03-06 Maria Monticelli, Bruno Hay Mele, Demi Marie Wright, Simone Guerriero, Giuseppina Andreotti, Maria Vittoria Cubellis
PMM2-CDG, a disease caused by mutations in phosphomannomutase-2, is the most common congenital disorder of glycosylation. Yet, it still lacks a cure. Targeting phosphomannomutase-2 with pharmacological chaperones or inhibiting the phosphatase activity of phosphomannomutase-1 to enhance intracellular glucose-1,6-bisphosphate have been proposed as therapeutical approaches.
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Enhancement of intrinsic guanine fluorescence by protonation in DNA of various structures Biochimie (IF 3.9) Pub Date : 2024-03-04 Liana L. Tevonyan, Natalia P. Bazhulina, Dmitry N. Kaluzhny
Understanding the diversity of DNA structure and functions in biology requires tools to study this biomolecule selectively and thoroughly. Fluorescence methods are powerful technique for non-invasive research. Due to the low quantum yield, the intrinsic fluorescence of nucleotides has not been considered for use in the detection and differentiation of nucleic acid bases. Here, we have studied the influence
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History of Tspo deletion and induction in vivo: Phenotypic outcomes under physiological and pathological situations Biochimie (IF 3.9) Pub Date : 2024-03-02 Juliette Bréhat, Leeyah Issop, Didier Morin
The mitochondrial translocator protein (TSPO) is an outer mitochondrial protein membrane with high affinity for cholesterol. It is expressed in most tissues but is more particularly enriched in steroidogenic tissues. TSPO is involved in various biological mechanisms and TSPO regulation has been related to several diseases. However, despite a considerable number of published studies interested in TSPO
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Biochemical and metabolic characterization of a G6PC2 inhibitor Biochimie (IF 3.9) Pub Date : 2024-03-01 Emily M. Hawes, Mohsin Rahim, Zeinab Haratipour, Abigail R. Orun, Margaret L. O'Rourke, James K. Oeser, Kwangho Kim, Derek P. Claxton, Ray D. Blind, Jamey D. Young, Richard M. O'Brien
Three glucose-6-phosphatase catalytic subunits, that hydrolyze glucose-6-phosphate (G6P) to glucose and inorganic phosphate, have been identified, designated G6PC1-3, but only G6PC1 and G6PC2 have been implicated in the regulation of fasting blood glucose (FBG). Elevated FBG has been associated with multiple adverse clinical outcomes, including increased risk for type 2 diabetes and various cancers
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Cytoskeletal β-tubulin and cysteine cathepsin L deregulation by SARS-CoV-2 spike protein interaction with the neuronal model cell line SH-SY5Y Biochimie (IF 3.9) Pub Date : 2024-03-01 Bernardo R. Oliveira, Inga Nehlmeier, Amy Madeleine Kempf, Vaishnavi Venugopalan, Maren Rehders, Marianne E.P. Ceniza, Pedro A.de T.P.V. Cavalcanti, Markus Hoffmann, Stefan Pöhlmann, Klaudia Brix
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Elucidating the pivotal role of TSPO in porphyrin-related cellular processes, in Bacillus cereus Biochimie (IF 3.9) Pub Date : 2024-02-27 Catherine Duport, Jean Armengaud, Caroline Schmitt, Didier Morin, Jean-Jacques Lacapère
A structural homolog of the mammalian TSPO has been identified in the human pathogen . BcTSPO, in its recombinant form, has previously been shown to bind and degrade porphyrins. In this study, we generated a Δ mutant strain in ATCC 14579 and assessed the impact of the absence of BcTSPO on cellular proteomics and physiological characteristics. The proteomic analysis revealed correlations between the
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Benefits of rutin on mitochondrial function and inflammation in an aluminum-induced neurotoxicity rat model: Potential interest for the prevention of neurodegeneration Biochimie (IF 3.9) Pub Date : 2024-02-24 Khadidja Kessas, Wafaa Lounis, Zehor Chouari, Anne Vejux, Gérard Lizard, Omar Kharoubi
Rutin, a phenolic compound, exhibits a diverse range of biological properties, including antioxidant, anti-inflammatory, and antimicrobial effects. In this study, we aimed to investigate the potential of rutin, a naturally occurring plant bioactive molecule, to mitigate the neurotoxic effects induced by aluminum chloride (AlCl). Over a period of 6 weeks, rats were intraperitoneally injected with AlCl
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Characterization of SLA RNA promoter from dengue virus and its interaction with the viral non-structural NS5 protein Biochimie (IF 3.9) Pub Date : 2024-02-24 Karl Brillet, Marta Janczuk-Richter, Amanda Poon, Joanne Laukart-Bradley, Eric Ennifar, Isabelle Lebars
The Dengue virus (DENV) is the most significant arthropod-borne viral pathogen in humans with 400 million infections annually. DENV comprises four distinct serotypes (DENV-1 to -4) which complicates vaccine development. Any of the four serotypes can cause clinical illness but with distinctive infection dynamics. Variations in sequences identified within the four genomes induce structural differences
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Pyridoxal kinase gene deletion leads to impaired growth, deranged redox metabolism and cell cycle arrest in Leishmania donovani Biochimie (IF 3.9) Pub Date : 2024-02-23 Pradyot Kumar Roy, Anindita Paul, Sandra Lalchhuanawmi, Neerupudi Kishore Babu, Sushma Singh
Pyridoxal kinase (PdxK) is a vitamin B salvage pathway enzyme which produces pyridoxal phosphate. We have investigated the impact of PdxK deletion in on parasite survivability, infectivity and cellular metabolism. PdxK mutants were generated by gene replacement strategy. All mutants showed significant reduction in growth in comparison to wild type. For PdxK mediated biochemical perturbations, only
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The ‘Not-So-Famous Five’ in tumorigenesis: tRNAs, tRNA fragments, and tRNA epitranscriptome in concert with AARSs and AIMPs Biochimie (IF 3.9) Pub Date : 2024-02-23 Sutapa Saha, Biyas Mukherjee, Proma Banerjee, Debadrita Das
RNA profiling studies have revealed that ∼75% of the human genome is transcribed to RNA but only a meagre fraction of it is translated to proteins. Majority of transcribed RNA constitute a specialized pool of non-coding RNAs. Human genome contains approximately 506 genes encoding a set of 51 different tRNAs, constituting a unique class of non-coding RNAs that not only have essential housekeeping functions
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Dioclea violacea lectin inhibits tumorigenesis and tumor angiogenesis in vivo Biochimie (IF 3.9) Pub Date : 2024-02-21 Abel Vieira de Melo Bisneto, Amanda Silva Fernandes, Lívia do Carmo Silva, Luana Santos Silva, Diego Pereira de Araújo, Ivan Cerqueira dos Santos, Marcella da Rocha Melo, Romério Rodrigues dos Santos Silva, Leonardo Pereira Franchi, Clever Gomes Cardoso, Elisangela de Paula Silveira-Lacerda, Cristiene Costa Carneiro, Claudener Souza Teixeira, Lee Chen-Chen
seed mannose-binding lectin (DvL) has attracted considerable attention because of its interesting biological activities, including antitumor, antioxidant, and anti-inflammatory activities. This study evaluated the cytotoxic effect of DvL on tumor and normal cells using the mitochondrial activity reduction (MTT) assay, the carcinogenic and anti-carcinogenic activity by the epithelial tumor test (ETT)
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The cellular SFPQ protein as a positive factor in the HIV-1 integration Biochimie (IF 3.9) Pub Date : 2024-02-17 Тatiana Kikhai, Yulia Agapkina, Maria Silkina, Tatiana Prikazchikova, Marina Gottikh
The cellular SFPQ protein is involved in several stages of the HIV-1 life cycle, but the detailed mechanism of its involvement is not yet fully understood. Here, the role of SFPQ in the early stages of HIV-1 replication has been studied. It is found that changes in the intracellular level of SFPQ affect the integration of viral DNA, but not reverse transcription, and SFPQ is a positive factor of integration
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Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site Biochimie (IF 3.9) Pub Date : 2024-02-13 Jeferson Stabile, Raquel Silva Neres-Santos, Isabela Dorta Molina Hernandes, Carolina Victória Cruz Junho, Geovane Felippe Alves, Isabella Cardoso Silva, Marcela Sorelli Carneiro-Ramos, Cristina Ribas Fürstenau
Acute kidney injury (AKI) is a public health problem and represents a risk factor for cardiovascular diseases (CVD) and vascular damage. This study aimed to investigate the impact of AKI on purinergic components in mice aorta. Main methods: The kidney ischemia was achieved by the occlusion of the left kidney pedicle for 60 min, followed by reperfusion for 8 (IR8) and 15 (IR15) days. Renal function
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Dynamics and metabolic effects of intestinal gases in healthy humans Biochimie (IF 3.9) Pub Date : 2024-02-05 Raffaele Minella
Many living beings use exogenous and/or endogenous gases to attain evolutionary benefits. We make a comprehensive assessment of one of the major gaseous reservoirs in the human body, i.e., the bowel, providing extensive data that may serve as reference for future studies. We assess the intestinal gases in healthy humans, including their volume, composition, source and local distribution in proximal
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Multi-oligomeric and catalytically compromised serine acetyltransferase and cysteine regulatory complex of Mycobacterium tuberculosis Biochimie (IF 3.9) Pub Date : 2024-02-03 Rahisuddin R, Payal Thakur, Narender Kumar, Neha Saini, Shrijta Banerjee, Ravi Pratap Singh, Madhuri Patel, S. Kumaran
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Role of the Escherichia coli FocA and FocB formate channels in controlling proton/potassium fluxes and hydrogen production during osmotic stress in energy-limited, stationary phase fermenting cells Biochimie (IF 3.9) Pub Date : 2024-02-01 A. Babayan, A. Vassilian, A. Poladyan, K. Trchounian
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Cell cycle and mitosis progression during ZIKA virus infection: The viral non-structural protein NS5 as a master regulator of the APC/cyclosome? Biochimie (IF 3.9) Pub Date : 2024-02-01 Grégorie Lebeau, Mathilde Hoareau, Sébastien Rivière, Daed El Safadi, Christine Robert Da Silva, Pascale Krejbich-Trotot, Wildriss Viranaicken
Alterations in cell cycle regulation contribute to Zika virus (ZIKV)-associated pathogenesis and may have implications for the development of therapeutic avenues. As a matter of fact, ZIKV alters cell cycle progression at multiple stages, including G1, S, G2, and M phases. During a cell cycle, the progression of mitosis is particularly controlled to avoid any abnormalities in cell division. In this
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Should the standard model of cellular energy metabolism be reconsidered? Possible coupling between the pentose phosphate pathway, glycolysis and extra-mitochondrial oxidative phosphorylation Biochimie (IF 3.9) Pub Date : 2024-02-01 Alessandro Maria Morelli, Felix Scholkmann
The process of cellular respiration occurs for energy production through catabolic reactions, generally with glucose as the first process step. In the present work, we introduce a novel concept for understanding this process, based on our conclusion that glucose metabolism is coupled to the pentose phosphate pathway (PPP) and extra-mitochondrial oxidative phosphorylation in a closed-loop process. According
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Isoprenylcysteine carboxyl methyltransferase (ICMT) promotes invadopodia formation and metastasis in cancer cells Biochimie (IF 3.9) Pub Date : 2024-01-30 Carla Borini Etichetti, Evelyn Arel Zalazar, Carolina Di Benedetto, Nabila Cocordano, Sabrina Valente, Silvio Bicciato, Mauricio Menacho-Márquez, María Cecilia Larocca, Javier Girardini
Isoprenyl cysteine carboxyl methyltransferase (ICMT) catalyzes the last step of the prenylation pathway. Previously, we found that high ICMT levels enhance tumorigenesis in vivo and that its expression is repressed by the p53 tumor suppressor. Based on evidence suggesting that some ICMT substrates affect invasive traits, we wondered if this enzyme may promote metastasis. In this work, we found that
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Translocator protein (TSPO) ligands attenuate mitophagy deficits in the SH-SY5Y cellular model of Alzheimer's disease via the autophagy adaptor P62 Biochimie (IF 3.9) Pub Date : 2024-01-26 Lauren H. Fairley, Amandine Grimm, Steffen A. Herff, Anne Eckert
Mitochondrial dysfunction has been widely implicated in the pathogenesis of Alzheimer's disease (AD), with accumulation of damaged and dysfunctional mitochondria occurring early in the disease. Mitophagy, which governs mitochondrial turnover and quality control, is impaired in the AD brain, and strategies aimed at enhancing mitophagy have been identified as promising therapeutic targets. The translocator
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Identification and characterisation of a novel interaction between oestrogen receptor alpha and FOXP2 Biochimie (IF 3.9) Pub Date : 2024-01-29 Aasiya Lakhi, Sylvia Fanucchi
Forkhead box P2 (FOXP2) regulates expression of various genes and is associated with language, speech and neural development as well as cancer. Since there may be a putative link between sex and language and because transcription factors rarely function in isolation, this study aims to investigate whether FOXP2 directly associates with oestrogen receptor α (ER1), a nuclear receptor responsible for
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Among the recombinant TSPOs, the BcTSPO Biochimie (IF 3.9) Pub Date : 2024-01-25 Leeyah Issop, Luminita Duma, Stephanie Finet, Olivier Lequin, Jean-Jacques Lacapère
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The rise of RNA: From fundamental research to therapeutic applications Biochimie (IF 3.9) Pub Date : 2024-01-23 Marie Sissler, Fabien Darfeuille
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Amplifying PCR productivity and environmental sustainability through shortened cycling protocols Biochimie (IF 3.9) Pub Date : 2024-01-21 Matthew Pedlar, Matthew J. Emery, Philip J. Warburton
Since its inception in the 1980s, advancements in PCR technology using improved thermal cyclers, engineered DNA polymerases and commercial master mixes, have led to increased PCR productivity. Despite these advancements, PCR cycling protocols have largely remained unchanged over the same period. This study aimed to systemically evaluate the effect of reduced PCR cycling parameters on amplicon production
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Deciphering the Akt1-HuD interaction in HuD-mediated neuronal differentiation Biochimie (IF 3.9) Pub Date : 2024-01-18 Hikari Nishisaka, Takumi Tomohiro, Kako Fukuzumi, Akira Fukao, Yoshinori Funakami, Toshinobu Fujiwara
The RNA-binding protein HuD/ELAVL4 is essential for neuronal development and synaptic plasticity by governing various post-transcriptional processes of target mRNAs, including stability, translation, and localization. We previously showed that the linker region and poly(A)-binding domain of HuD play a pivotal role in promoting translation and inducing neurite outgrowth. In addition, we found that HuD
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Discovery and characterization of the α-amylases cDNAs from Enchytraeus albidus shed light on the evolution of “Enchytraeus-Eisenia type” Amy homologs in Annelida Biochimie (IF 3.9) Pub Date : 2024-01-17 Łukasz Gajda, Agata Daszkowska-Golec, Piotr Świątek
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Dynamic modulation of transthylakoid electric potential by chloroplast ATP synthases Biochimie (IF 3.9) Pub Date : 2024-01-13 Hui Lyu, Yong-Song Zuo
The light-induced transthylakoid membrane potential (ΔΨm) can function as a driving force to help catalyzing the formation of ATP molecules, proving a tight connection between ΔΨm and the ATP synthase. Naturally, a question can be raised on the effects of altered functioning of ATP synthases on regulating ΔΨm, which is attractive in the area of photosynthetic research. Lots of findings, when making
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Cell-penetrating peptide and cationic liposomes mediated siRNA delivery to arrest growth of chronic myeloid leukemia cells in vitro Biochimie (IF 3.9) Pub Date : 2024-01-10 Vera Vysochinskaya, Yana Zabrodskaya, Olesya Dovbysh, Anton Emelyanov, Vladimir Klimenko, Nikolay Knyazev, Ivan Terterov, Marya Egorova, Alexey Bogdanov, Michael Maslov, Andrey Vasin, Michael Dubina
Gene silencing through RNA interference (RNAi) is a promising therapeutic approach for a wide range of disorders, including cancer. Non-viral gene therapy, using specific siRNAs against BCR-ABL1, can be a supportive or alternative measure to traditional chronic myeloid leukemia (CML) tyrosine kinase inhibitor (TKIs) therapies, given the prevalence of clinical TKI resistance. The main challenge for
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Sucrose phosphorylase from Alteromonas mediterranea: Structural insight into the regioselective α-glucosylation of (+)-catechin Biochimie (IF 3.9) Pub Date : 2024-01-09 Marine Goux, Marie Demonceaux, Johann Hendrickx, Claude Solleux, Emilie Lormeau, Folmer Fredslund, David Tezé, Bernard Offmann, Corinne André-Miral
Sucrose phosphorylases, through transglycosylation reactions, are interesting enzymes that can transfer regioselectively glucose from sucrose, the donor substrate, onto acceptors like flavonoids to form glycoconjugates and hence modulate their solubility and bioactivity. Here, we report for the first time the structure of sucrose phosphorylase from the marine bacteria Alteromonas mediterranea (AmSP)
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Exploring NAD+ metabolism and NNAT: Insights from structure, function, and computational modeling Biochimie (IF 3.9) Pub Date : 2024-01-03 Olamide Jeje, Sarah Otun, Chinyere Aloke, Ikechukwu Achilonu
Nicotinamide Adenine Dinucleotide (NAD+), a coenzyme, is ubiquitously distributed and serves crucial functions in diverse biological processes, encompassing redox reactions, energy metabolism, and cellular signalling. This review article explores the intricate realm of NAD + metabolism, with a particular emphasis on the complex relationship between its structure, function, and the pivotal enzyme, Nicotinate
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Recent advances in nano/microfluidics-based cell isolation techniques for cancer diagnosis and treatments Biochimie (IF 3.9) Pub Date : 2024-01-03 Nahid Shanehband, Seyed Morteza Naghib
Miniaturization has improved significantly in the recent decade, which has enabled the development of numerous microfluidic systems. Microfluidic technologies have shown great potential for separating desired cells from heterogeneous samples, as they offer benefits such as low sample consumption, easy operation, and high separation accuracy. Microfluidic cell separation approaches can be classified
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The determinants of metabolic discrepancies in aerobic glycolysis: Providing potential targets for breast cancer treatment Biochimie (IF 3.9) Pub Date : 2024-01-04 Ajeesh Babu Littleflower, Sulfath Thottungal Parambil, Gisha Rose Antony, Lakshmi Subhadradevi
Altered aerobic glycolysis is the robust mechanism to support cancer cell survival and proliferation beyond the maintenance of cellular energy metabolism. Several investigators portrayed the important role of deregulated glycolysis in different cancers, including breast cancer. Breast cancer is the most ubiquitous form of cancer and the primary cause of cancer death in women worldwide. Breast cancer
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The complex repertoire of Tityus spp. venoms: Advances on their composition and pharmacological potential of their toxins Biochimie (IF 3.9) Pub Date : 2024-01-03 Gisele A. Wiezel, Isadora S. Oliveira, Mouzarllem B. Reis, Isabela G. Ferreira, Kalynka R. Cordeiro, Karla C.F. Bordon, Eliane C. Arantes
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Comparison of the calcium signaling alterations in GABA-ergic medium spiny neurons produced from iPSCs of different origins Biochimie (IF 3.9) Pub Date : 2023-12-30 Arina A. Oshkolova, Dmitriy A. Grekhnev, Anna A. Kruchinina, Lilia D. Belikova, Egor A. Volovikov, Olga S. Lebedeva, Alexandra N. Bogomazova, Vladimir A. Vigont, Maria A. Lagarkova, Elena V. Kaznacheyeva
Disease models based on induced pluripotent stem cells (iPSCs) are in high demand because of their physiological adequacy and well-reproducibility of the pathological phenotype. Nowadays, the most common approach to generate iPSCs is the reprogramming of somatic cells using vectors based on lentivirus or Sendai virus. We have previously shown impairments of calcium signaling including store-operated
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Unlocking the enigma of phenotypic drug tolerance: Mechanisms and emerging therapeutic strategies Biochimie (IF 3.9) Pub Date : 2023-12-31 Alok K. Mishra, Ritesh P. Thakare, Bela G. Santani, Shivraj M. Yabaji, Shivendra K. Dixit, Kishore K. Srivastava
In the ongoing battle against antimicrobial resistance, phenotypic drug tolerance poses a formidable challenge. This adaptive ability of microorganisms to withstand drug pressure without genetic alterations further complicating global healthcare challenges. Microbial populations employ an array of persistence mechanisms, including dormancy, biofilm formation, adaptation to intracellular environments
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Potential elevation of exopeptidase activity of Glu-specific endopeptidase I/GluV8 mediated by hydrophobic P1′-position amino acid residue Biochimie (IF 3.9) Pub Date : 2023-12-28 Takayuki K. Nemoto, Haruka Nishimata, Kana Shirakura, Yuko Ohara-Nemoto
We recently reported that the activities of dipeptidyl-peptidase (DPP)7 and DPP11, S46-family exopeptidases were significantly elevated by the presence of prime-side amino acid residues of substrates caused by an increase in kcat [Ohara-Nemoto Y. et al., J Biol Chem 298(3):101585. doi: 10.1016/j.jbc.2022]. In the present study, the effects of prime-side residues on Glu-specific endopeptidase I/GluV8
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Purification and characterization of Cdr1, the drug-efflux pump conferring azole resistance in Candida species Biochimie (IF 3.9) Pub Date : 2023-12-27 Jorgaq Pata, Alexis Moreno, Benjamin Wiseman, Sandrine Magnard, Idriss Lehlali, Marie Dujardin, Atanu Banerjee, Martin Högbom, Ahcène Boumendjel, Vincent Chaptal, Rajendra Prasad, Pierre Falson
Candida albicans and C. glabrata express exporters of the ATP-binding cassette (ABC) superfamily and address them to their plasma membrane to expel azole antifungals, which cancels out their action and allows the yeast to become multidrug resistant (MDR). In a way to understand this mechanism of defense, we describe the purification and characterization of Cdr1, the membrane ABC exporter mainly responsible
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Naked mole-rat hyaluronan Biochimie (IF 3.9) Pub Date : 2023-12-27 Francisco Alejandro Lagunas-Rangel
Naked mole rats (NMRs) are renowned for their exceptional longevity and remarkable maintenance of health throughout their lifetime. Their subterranean lifestyle has led to adaptations that have resulted in elevated levels of a very high molecular weight hyaluronan in their tissues. Hyaluronan, a glycosaminoglycan, is a key component of the extracellular matrix, which plays a critical role in maintaining
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Leishmania donovani mevalonate kinase regulates host actin for inducing phagocytosis Biochimie (IF 3.9) Pub Date : 2023-12-19 Tanvir Bamra, Taj Shafi, Sushmita Das, Manjay Kumar, Pradeep Das
Despite the well-established role of macrophages in phagocytosing Leishmania, the contribution of the parasite to this process is not well understood. Present study provides insights into the mechanism underlying the MVK-induced entry of L. donovani and improve our knowledge of host-pathogen interactions. We have discussed Mevalonate kinase (MVK)-induced actin reorganization, modulation of signaling
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Effects of osmolytes under crowding conditions on the properties of muscle glycogen phosphorylase b Biochimie (IF 3.9) Pub Date : 2023-12-20 Valeriya V. Mikhaylova, Tatiana B. Eronina
The study of the relationship between the activity and stability of enzymes under crowding conditions in the presence of osmolytes is important for understanding the functioning of a living cell. The effect of osmolytes (trehalose and betaine) on the secondary and tertiary structure and activity of muscle glycogen phosphorylase b (Phb) under crowding conditions created by PEG 2000 and PEG 20000 was
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Sensing of DNA modifications by pAgo proteins in vitro Biochimie (IF 3.9) Pub Date : 2023-12-19 Margarita Beskrovnaia, Aleksei Agapov, Kristina Makasheva, Dmitry O. Zharkov, Daria Esyunina, Andrey Kulbachinskiy
Many prokaryotic Argonaute (pAgo) proteins act as programmable nucleases that use small guide DNAs for recognition and cleavage of complementary target DNA. Recent studies suggested that pAgos participate in cell defense against invader DNA and may also be involved in other genetic processes, including DNA replication and repair. The ability of pAgos to recognize specific targets potentially make them
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Glutathione is required for growth and cadmium tolerance in the amphibian chytrid fungus, Batrachochytrium dendrobatidis Biochimie (IF 3.9) Pub Date : 2023-12-16 Rebecca J. Webb, Catherine Rush, Lee Berger, Lee F. Skerratt, Alexandra A. Roberts
Batrachochytrium dendrobatidis (Bd) is a lethal amphibian pathogen, partly due to its ability to evade the immune system of susceptible frog species. In many pathogenic fungi, the antioxidant glutathione is a virulence factor that helps neutralise oxidative stressors generated from host immune cells, as well as other environmental stressors such as heavy metals. The role of glutathione in stress tolerance