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Interferon inducible guanylate‐binding protein 1 modulates the lipopolysaccharide‐induced cytokines/chemokines and mitogen‐activated protein kinases in macrophages Microbiol. Immunol. (IF 2.6) Pub Date : 2024-03-11 Ravindra Kumar, Pramod Kumar Kushawaha
Guanylate‐binding proteins (GBPs) are a family of interferon (IFN)‐inducible GTPases and play a pivotal role in the host immune response to microbial infections. These are upregulated in immune cells after recognizing the lipopolysaccharides (LPS), the major membrane component of Gram‐negative bacteria. In the present study, the expression pattern of GBP1–7 was initially mapped in phorbol 12‐myristate
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N‐glycosylation of the SARS‐CoV‐2 spike protein at Asn331 and Asn343 is involved in spike‐ACE2 binding, virus entry, and regulation of IL‐6 Microbiol. Immunol. (IF 2.6) Pub Date : 2024-03-06 Tuhin Das, Shuhong Luo, Hao Tang, Jianmin Fang, Yinging Mao, Haw‐Han Yen, Sabyasachi Dash, Asif Shajahan, Lauren Pepi, Steven Huang, Valerie S. Jones, Shehuo Xie, Gordon F. Huang, Jinqiao Lu, Blake Anderson, Benyue Zhang, Parastoo Azadi, Ruo‐Pan Huang
The coronavirus disease 2019 (COVID‐19) pandemic is an ongoing global public health crisis. The causative agent, the SARS‐CoV‐2 virus, enters host cells via molecular interactions between the viral spike protein and the host cell ACE2 surface protein. The SARS‐CoV‐2 spike protein is extensively decorated with up to 66 N‐linked glycans. Glycosylation of viral proteins is known to function in immune
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Establishment of COS‐BK cells persistently infected with archetype BK polyomavirus Microbiol. Immunol. (IF 2.6) Pub Date : 2024-03-04 Souichi Nukuzuma, Hiroshi Onogi, Tetsuro Suzuki
BK polyomavirus (BKPyV) was the first human polyomavirus to be isolated from an immunosuppressed kidney transplant recipient in 1971. BKPyV reactivation causes BKPyV‐associated nephropathy and hemorrhagic cystitis. However, the mechanisms underlying BKPyV replication remain unclear. In the present study, we performed the long‐term cultivation of COS‐7 cells transfected with archetype KOM‐5 DNA, which
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Rapid quantitative detection system for measles virus‐neutralizing antibodies using HiBiT‐tagged virus‐like particles Microbiol. Immunol. (IF 2.6) Pub Date : 2024-02-28 Takashi Okura, Kei Miyakawa, Maino Tahara, Kenji Someya, Fumio Seki, Mayuko Nishi, Noriyuki Otsuki, Akihide Ryo
Immunological testing to detect neutralizing antibodies (NAbs) is important in measles (MV) infection control. Currently, the plaque reduction neutralization test is the only credible method for measuring actual virus NAbs; however, its feasibility is hampered by drawbacks, such as long turnaround times, low throughput, and the need for laboratory biosafety equipment. To solve these problems, we developed
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MYBBP1A is required for efficient replication and gene expression of herpes simplex virus 1 Microbiol. Immunol. (IF 2.6) Pub Date : 2024-02-25 Moeka Nobe, Yuhei Maruzuru, Kosuke Takeshima, Naoto Koyanagi, Akihisa Kato, Yasushi Kawaguchi
More than 100 different herpes simplex virus 1 (HSV‐1) genes belong to three major classes, and their expression is coordinately regulated and sequentially ordered in a cascade. This complex HSV‐1 gene expression is thought to be regulated by various viral and host cellular proteins. A host cellular protein, Myb‐binding protein 1A (MYBBP1A), has been reported to be associated with HSV‐1 viral genomes
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Current understanding of Bordetella-induced cough Microbiol. Immunol. (IF 2.6) Pub Date : 2024-02-06 Yasuhiko Horiguchi
Typical pathogenic bacteria of the genus Bordetella cause respiratory diseases, many of which are characterized by severe coughing in host animals. In human infections with these bacteria, such as whooping cough, coughing imposes a heavy burden on patients. The pathophysiology of this severe coughing had long been uncharacterized because convenient animal models that reproduce Bordetella-induced cough
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Lipoprotein signal peptidase-deficient Streptococcus pneumoniae exhibits impaired Toll-like receptor 2-stimulatory activity Microbiol. Immunol. (IF 2.6) Pub Date : 2024-02-04 Hisanori Domon, Satoru Hirayama, Toshihito Isono, Rui Saito, Katsunori Yanagihara, Yutaka Terao
Streptococcus pneumoniae is a causative agent of community-acquired pneumonia. Upon pneumococcal infection, innate immune cells recognize pneumococcal lipoproteins via Toll-like receptor 2 and induce inflammation. Here, we generated a strain of S. pneumoniae deficient in lipoprotein signal peptidase (LspA), a transmembrane type II signal peptidase required for lipoprotein maturation, to investigate
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Novel role of host protein SLC25A42 in the HIV-1 reactivation of latent HIV-1 provirus Microbiol. Immunol. (IF 2.6) Pub Date : 2024-01-20 Kei Taga, Hiroaki Takeuchi
Despite the effectiveness of combination antiretroviral therapy, human immunodeficiency virus (HIV) infection remains incurable. To seek new strategies to overcome HIV type 1 (HIV-1) latency, one of the major barriers to HIV elimination, it is crucial to better understand how this state is maintained. Here, by means of an RNA interference screen employing an HIV-1 latency model using monocytic cell
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Isolation and whole-genome sequencing analysis of Escherichia fergusonii harboring a heat-labile enterotoxin gene from retail chicken meat in Okinawa, Japan Microbiol. Immunol. (IF 2.6) Pub Date : 2024-01-20 Tetsuya Kakita, Kenichi Lee, Masatomo Morita, Miki Okuno, Hisako Kyan, Sho Okano, Noriyuki Maeshiro, Momoko Ishizu, Tsuyoshi Kudeken, Haruno Taira, Morimi Teruya, Yoshitoshi Ogura, Yukihiro Akeda, Makoto Ohnishi
This study aimed to reveal the prevalence of heat-labile enterotoxin (LT) gene-positive Escherichia fergusonii in retail chicken meat and genetically characterize these strains. E. fergusonii harboring LT gene was isolated from 6 out of 60 (10%) retail chicken samples in Okinawa, Japan. Whole-genome sequencing analysis revealed that LT gene-positive E. fergusonii from chicken meat and feces contain
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Oral and rectal colonization of methicillin-resistant Staphylococcus aureus in long-term care facility residents and their association with clinical status Microbiol. Immunol. (IF 2.6) Pub Date : 2024-01-17 Satoru Kusaka, Azusa Haruta, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Mineka Yoshikawa, Toshiki Kajihara, Koji Yahara, Junzo Hisatsune, Ryota Nomura, Kazuhiro Tsuga, Hiroki Ohge, Motoyuki Sugai, Hitoshi Komatsuzawa
Staphylococcus aureus is a commensal bacterium in humans, but it sometimes causes opportunistic infectious diseases such as suppurative skin disease, pneumonia, and enteritis. Therefore, it is important to determine the prevalence of S. aureus and methicillin-resistant S. aureus (MRSA) in individuals, especially older adults. In this study, we investigated the prevalence of S. aureus and MRSA in the
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Strain identity of Bordetella pertussis isolates from household members based on whole-genome sequencing Microbiol. Immunol. (IF 2.6) Pub Date : 2024-01-15 Kazunari Kamachi, Kentaro Koide, Nao Otsuka, Masataka Goto, Tsuyoshi Kenri
We evaluated the genetic diversity of Bordetella pertussis, the causative agent of pertussis, within households by whole-genome sequencing. In pairwise comparisons of 23 isolates collected from 11 households, single-nucleotide polymorphism (SNP) analysis revealed extremely low SNP diversity (≤1 SNP) between isolate pairs: no SNPs were detected in 10 households and one SNP was obtained in the remaining
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Circ_0008410 contributes to fibroblast-like synoviocytes dysfunction by regulating miR-149-5p/HIPK2 axis Microbiol. Immunol. (IF 2.6) Pub Date : 2023-12-21 Wensi Su, Zhifang Ye, Guangji Wang, Hui Huang, Yehan Fang
Circular RNAs (circRNAs) play functional roles in rheumatoid arthritis (RA) progression. Fibroblast-like synoviocytes (RASFs) are the main effectors in RA development. In this study, we explored the function and mechanism of circ_0008410 in RASFs. qRT-PCR was used to detect the expression of circ_0008410, microRNA-149-5p (miR-149-5p), and homeodomain-interacting protein kinase 2 (HIPK2). Cell counting
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RpoN (sigma factor 54) contributes to bacterial fitness during tracheal colonization of Bordetella bronchiseptica Microbiol. Immunol. (IF 2.6) Pub Date : 2023-12-17 Xingyan Ma, Dendi K. Nugraha, Yukihiro Hiramatsu, Yasuhiko Horiguchi
The Gram-negative pathogenic bacterium Bordetella bronchiseptica is a respiratory pathogen closely related to Bordetella pertussis, the causative agent of whooping cough. Despite sharing homologous virulence factors, B. bronchiseptica infects a broad range of mammalian hosts, including some experimental animals, whereas B. pertussis is strictly adapted to humans. Therefore, B. bronchiseptica is often
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HPV vaccines induce trained immunity and modulate pro-inflammatory cytokine expression in response to secondary Toll-like receptor stimulations Microbiol. Immunol. (IF 2.6) Pub Date : 2023-12-17 Mako Yamaguchi, Yohana S. Mtali, Hitomi Sonokawa, Ken Takashima, Yoshimi Fukushima, Takahisa Kouwaki, Hiroyuki Oshiumi
Cervical cancer is caused mostly by human papillomavirus (HPV), and several HPV vaccines have been developed to prevent its onset. Vaccines include antigens as well as adjuvants, with adjuvants playing an important role in activating the innate immune responses necessary for inducing adaptive immunological responses. Recent research has shown the presence of trained immunity inside the innate immune
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Development of recombinant rotavirus carrying herpes simplex virus 2 glycoprotein D gene based on reverse genetics technology Microbiol. Immunol. (IF 2.6) Pub Date : 2023-12-14 Yoshiki Kawamura, Satoshi Komoto, Saori Fukuda, Masanori Kugita, Shuang Tang, Amita Patel, Julianna R. Pieknik, Shizuko Nagao, Koki Taniguchi, Philip R. Krause, Tetsushi Yoshikawa
Vaccine development for herpes simplex virus 2 (HSV-2) has been attempted, but no vaccines are yet available. A plasmid-based reverse genetics system for Rotavirus (RV), which can cause gastroenteritis, allows the generation of recombinant RV containing foreign genes. In this study, we sought to develop simian RV (SA11) as a vector to express HSV-2 glycoprotein D (gD2) and evaluated its immunogenicity
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Azithromycin regulates bacterial virulence and immune response in a murine model of ceftazidime-treated Pseudomonas aeruginosa acute pneumonia Microbiol. Immunol. (IF 2.6) Pub Date : 2023-12-10 A.-G. Leroy, J. Caillon, A. Broquet, V. Lemabecque, S. Delanou, N. Caroff, K. Asehnoune, A. Roquilly, L. Crémet
Pseudomonas aeruginosa (PA) remains one of the leading causes of nosocomial acute pneumonia. The array of virulence factors expressed by PA and the intense immune response associated with PA pneumonia play a major role in the severity of these infections. New therapeutic approaches are needed to overcome the high resistance of PA to antibiotics and to reduce the direct damage to host tissues. Through
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Association of ATG16L1 and ATG5 gene polymorphisms with susceptibility to hepatitis B virus infection and progression to HCC in central China Microbiol. Immunol. (IF 2.6) Pub Date : 2023-11-22 Qiaoyu Wu, Yaoling Ouyang
Hepatitis B virus (HBV) infection is a severe public health problem worldwide. The relationship between polymorphisms of autophagy-related 16-like 1 gene (ATG16L1) and autophagy-related gene 5 (ATG5) with susceptibility to the stage of HBV infection has been reported in different populations. Nevertheless, this association is not seen in the population of central China. This study recruited 452 participants
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Biological characteristics and pathogenicity of a Staphylococcus aureus strain with an incomplete hemolytic phenotype isolated from bovine milk Microbiol. Immunol. (IF 2.6) Pub Date : 2023-11-20 Xiuhua Xu, Tingting Zhou, Xueyao Fang, Longhua Hu, Jin Zhu, Feng Zheng
Staphylococcus aureus is a common pathogen capable of infecting both humans and animals and causing various severe diseases. Here, we aimed to determine the biological features and pathogenicity of S. aureus strain Sa9, of the incomplete hemolysis phenotype, isolated from bovine milk. Sa9 was classified as ST97 by multilocus sequence typing, and it showed increased β-hemolysin expression and lower
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Pneumococcus downregulates the molecular weight of the extracellular domain of the epidermal growth factor receptor of alveolar epithelial cells Microbiol. Immunol. (IF 2.6) Pub Date : 2023-11-17 Toshihito Isono, Satoru Hirayama, Hisanori Domon, Yutaka Terao
Pneumococcus is themajor cause of bacterial and invasive pneumococcal infections. Disrupting the alveolarepithelial barrier is an important step in the pathogenesis of invasivepneumococcal infections. The epidermal growth factor receptor (EGFR) maintainsthe integrity of the alveolar epithelial barrier. In this study, we showed that secretory pneumococcal molecules decrease the molecular weight of EGFR
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Modulatory effects of supplementation of Lentinula edodes mycelia extract and l-arginine on the therapeutic efficacy of immunogenic chemotherapy in colon cancer-bearing mice Microbiol. Immunol. (IF 2.6) Pub Date : 2023-11-14 Takahito Taniura, Kazunari Ishitobi, Masaaki Hidaka, Mamoru Harada
Some chemotherapeutic drugs can induce cancer cell death and enhance antitumor T-cell immunity in cancer-bearing hosts. Immunomodulatory reagents could augment such chemotherapy-induced effects. We previously reported that oral digestion of Lentinula edodes mycelia (L.E.M.) extract or l-arginine supplementation can augment antitumor T-cell responses in cancer-bearing mice. In this study, the effects
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Genomic characterization of New Delhi metallo-beta-lactamase–producing species of Morganellaceae, Yersiniaceae, and Enterobacteriaceae (other than Klebsiella) from Brazil over 2013–2022 Microbiol. Immunol. (IF 2.6) Pub Date : 2023-10-19 Carlos Henrique Camargo, Amanda Yaeko Yamada, Andreia Rodrigues de Souza, Claudio Tavares Sacchi, Alex Domingos Reis, Marlon Benedito Nascimento Santos, Denise Brandão de Assis, Eneas de Carvalho, Elizabeth Harummyy Takagi, Marcos Paulo Vieira Cunha, Monique Ribeiro Tiba-Casas
Over the last decade, New Delhi metallo-beta-lactamase (NDM) carbapenemase has silently spread in Brazil. In this study, we analyzed a large collection of Enterobacterales other than Klebsiella spp. received in our reference laboratory between 2013 and 2022. A total of 32 clinical isolates displaying different pulsed-field gel electrophoresis profiles, and represented by 11 species in the families
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Natto extract inhibits infection caused by the Aujeszky's disease virus in mice Microbiol. Immunol. (IF 2.6) Pub Date : 2023-10-10 Junya Kobayashi, Rongduo Wen, Takanobu Nishikawa, Yuka Nunomura, Takehito Suzuki, Yudai Sejima, Toshiya Gokan, Makio Furukawa, Tomoko Yokota, Nanako Osawa, Yoko Sato, Yutaka Nibu, Tetsuya Mizutani, Mami Oba
Aujeszky's disease virus (ADV), also known as Suid alphaherpesvirus 1, which mainly infects swine, causes life-threatening neurological disorders. This disease is a serious global risk factor for economic losses in the swine industry. The development of new anti-ADV drugs is highly anticipated and required. Natto, a traditional Japanese fermented food made from soybeans, is a well-known health food
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Bioinformatic investigation of Nipah virus surface protein mutations: Molecular docking with Ephrin B2 receptor, molecular dynamics simulation, and structural impact analysis Microbiol. Immunol. (IF 2.6) Pub Date : 2023-10-09 Emre Aktaş, İrem Saygılı, Elif Kahveci, Zeynep Tekbıyık, Nehir Özdemir Özgentürk
The SARS-CoV-2 outbreak resulted in significant challenges and loss of life. The Nipah virus, known for its high infectivity and severity, was designated an emergency concern by the World Health Organization. To understand its mutations, the Nipah virus proteins were analyzed extensively, with a focus on the essential G and F proteins responsible for viral entry into host cells. Our bioinformatics
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Siderophore-producing Pantoea ferrattrahens sp. nov. isolated from a clinical specimen and Pantoea ferramans sp. nov. isolated from soil at the bottom of a pond Microbiol. Immunol. (IF 2.6) Pub Date : 2023-09-22 Ryo Kutsuna, Tohru Miyoshi-Akiyama, Yuki Muramatsu, Moriyuki Hamada, Junko Tomida, Ken Kikuchi, Yoshiaki Kawamura
Two Gram-negative facultative anaerobes were isolated from a sepsis patient with pancreatic cancer (strain PAGU 2156T) and soil at the bottom of a pond (strain PAGU 2198T), respectively. These two strains formed haloes around the colonies on chrome azurol S agar plates, indicating the production of siderophores. Two isolates assigned to the genus Pantoea based on the 16S rRNA gene were differentiated
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Issue Information – Cover Microbiol. Immunol. (IF 2.6) Pub Date : 2023-09-04
Cover photograph: Circular map of the genome of Acinetobacter baumannii CYZ. Microbiol Immunol: 67:396–403. Article link here
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Expression of macrophage/dendritic cell–related molecules in lymph node sinus macrophages Microbiol. Immunol. (IF 2.6) Pub Date : 2023-08-25 Rin Yamada, Koji Ohnishi, Cheng Pan, Hiromu Yano, Yukio Fujiwara, Takuya Shiota, Yoshiki Mikami, Yoshihiro Komohara
The role of sinus macrophages (SMs) in anticancer immune responses has received considerable interest in recent years, but the types of molecules that are expressed in human SMs have not yet been clarified in detail. We therefore sought to identify dendritic cell (DC)– or macrophage-related molecules in SMs in human lymph nodes (LNs). SMs are strongly positive for Iba-1, CD163, CD169, and CD209. CD169
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TLR2 mediates autophagy through ERK signaling pathway in Chlamydia psittaci CPSIT_p7 protein-stimulated RAW264.7 cells Microbiol. Immunol. (IF 2.6) Pub Date : 2023-08-24 Ying Luo, Zhenjie Sun, Qian Chen, Jian Xiao, XiaoLiang Yan, Yumeng Li, Yimou Wu
Chlamydia psittaci is a zoonotic pathogen found in birds and humans. Macrophages, major components of the innate immune system, can resist chlamydial infections and trigger adaptive immune responses. However, the molecular mechanisms underlying the action of macrophages against C. psittaci infection are not well understood. This study investigated the roles and mechanisms of plasmid-encoded protein
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A multilocus sequence typing method of Staphylococcus aureus DNAs in a sample from human skin Microbiol. Immunol. (IF 2.6) Pub Date : 2023-08-13 Hiroka Furuya, Kohei Ogura, Norihiko Takemoto, Shinya Watanabe, Ayaka Yamazaki, Kazuhiro Ogai, Junko Sugama, Shigefumi Okamoto
The skin and mucous membranes are the primary sites of Staphylococcus aureus colonization, particularly those of health care personnel and patients in long-term care centers. We found that S. aureus colonized with a higher abundance ratio on skins which had recovered from pressure injury (PI) than on normal skins in our earlier research on the skin microbiota of bedridden patients. Multilocus sequence
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Issue Information – Cover Microbiol. Immunol. (IF 2.6) Pub Date : 2023-08-04
Cover photograph: Metabolism pathways and epigenetic reprogramming during immune memory. The primary infection triggers the activation of intracellular metabolism pathways such as glycolysis and adenosine metabolism. Some metabolites of these processes, such as acetyl coenzyme A (acetyl-CoA) and S-adenosylmethionine (SAM), might mediate epigenetic reprogramming of gene regulation during innate immune
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Filipendula glaberrima Nakai extract inhibits the bacterial infection by induction of HBD2 and HBD3 expression, and reduction of the inflammatory activity Microbiol. Immunol. (IF 2.6) Pub Date : 2023-07-31 Anh-Thu Nguyen, Minho Kim, Ye-Eun Kim, Hangeun Kim, Ki-Young Kim
Defensins and inflammation are innate immune barriers of the body against infectious pathogens. Searching for a compound that can inhibit infectious diseases by affecting human β-defensin (HBD) and proinflammatory cytokines is the new trend in research to control bacterial infection. The aim of this study is to provide a natural compound, Filipendula glaberrima Nakai extract (FGE), which is able to
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Ceramic absorbed with calcium bicarbonate mesoscopic crystals partially inactivate scrapie prions Microbiol. Immunol. (IF 2.6) Pub Date : 2023-07-30 Yoshifumi Iwamaru, Koichi Furusaki, Katsuaki Sugiura, Makoto Haritani, Takashi Onodera
Prion diseases are fatal neurodegenerative disorders affecting both humans and animals. The causative agent, prion, is extremely resistant to common disinfection procedures. Thus, effective prion inactivation strategies using relatively safe and less corrosive disinfectants are required. A solution containing CAC-717, mesoscopic crystals of calcium bicarbonate, exerts both antimicrobial and virucidal
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Activation of inflammasomes and mechanisms for intracellular recognition of Listeria monocytogenes Microbiol. Immunol. (IF 2.6) Pub Date : 2023-07-18 Yasuyuki Matsuda, Hajime Yamauchi, Hideki Hara
The high mortality rate associated with Listeria monocytogenes can be attributed to its ability to invade the body systemically and to activate inflammasomes. Both of these processes are facilitated by expressing a major virulence factor known as listeriolysin O, a 56 kDa pore-forming protein encoded by the hly gene. Listeriolysin O plays a crucial role in the pathogenesis of the bacterium by facilitating
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Rheotaxis in Mycoplasma gliding Microbiol. Immunol. (IF 2.6) Pub Date : 2023-07-10 Daisuke Nakane
This review describes the upstream-directed movement in the small parasitic bacterium Mycoplasma. Many Mycoplasma species exhibit gliding motility, a form of biological motion over surfaces without the aid of general surface appendages such as flagella. The gliding motility is characterized by a constant unidirectional movement without changes in direction or backward motion. Unlike flagellated bacteria
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Knock-out of multidrug efflux pump MexXY-OprM results in increased susceptibility to antimicrobial peptides in Pseudomonas aeruginosa Microbiol. Immunol. (IF 2.6) Pub Date : 2023-07-09 Anke Neidig, Nikola Strempel, Nadine Bianca Waeber, Waleska Stephanie da Cruz Nizer, Joerg Overhage
Multidrug efflux systems of the resistance-nodulation-cell division family play a crucial role in resistance of Pseudomonas aeruginosa to a large variety of antibiotics. Here, we investigated the role of clinically relevant efflux pumps MexAB−OprM, MexCD−OprJ, and MexXY−OprM in resistance against different cationic antimicrobial peptides (AMPs). Our results indicate that a knock-out in efflux pump
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Establishment of a new reverse genetics system for respiratory syncytial virus under the control of RNA polymerase II Microbiol. Immunol. (IF 2.6) Pub Date : 2023-07-09 Tatsuki Takahashi, Shiori Ueno, Yoshiro Sugiura, Kenta Shimizu, Wataru Kamitani
A reverse genetics system for the respiratory syncytial virus (RSV), which causes acute respiratory illness, is an effective tool for understanding the pathogenicity of RSV. To date, a method dependent on T7 RNA polymerase is commonly used for RSV. Although this method is well established and recombinant RSV is well rescued from transfected cells, the requirement for artificial supply of T7 RNA polymerase
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Investigating theobromine as a potential anti-human coronaviral agent Microbiol. Immunol. (IF 2.6) Pub Date : 2023-07-06 Jiajing Li, Yining Wang, Sajjan Rajpoot, Marla Lavrijsen, Qiuwei Pan, Pengfei Li, Mirza S. Baig
Coronaviruses (CoVs) have long been known to infect humans, mainly alpha-CoV and beta-CoV. The vaccines developed for SARS-CoV-2 are likely not effective against other coronavirus species, whereas the risk of the emergence of new strains that may cause the next epidemic/pandemic is high. The development of antiviral drugs that are effective across different CoVs represents a viable strategy for improving
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Analysis of multidrug-resistant determinants of clinically isolated Acinetobacter baumannii CYZ via whole genome sequencing Microbiol. Immunol. (IF 2.6) Pub Date : 2023-07-04 Xia Chuai, Yaya Zhou, Junhua Feng, Menghan Yu, Yan Wu, Lujuan Han, Yan Zhao, Hongxiu Qiao, Zhiyun Gao, Jian Li, Lixin Xie, Wenting Zhao, Changle Wang
Acinetobacter baumannii is a multidrug-resistant coccobacillus responsible for severe nosocomial infectious diseases. This study mainly focuses on investigating the antimicrobial resistance features of a clinically isolated strain (A. baumannii CYZ) using the PacBio Sequel II sequencing platform. The chromosomal size of A. baumannii CYZ is 3,960,760 bp, which contains a total of 3803 genes with a G + C
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Nicotine promotes Mycobacterium tuberculosis H37Rv growth and overexpression of virulence genes Microbiol. Immunol. (IF 2.6) Pub Date : 2023-06-28 Bruno Rivas-Santiago, Jeny de Haro-Acosta, Adrian Rodríguez Carlos, Mariana H. Garcia-Hernandez, Carmen J. Serrano, Irma Gonzalez-Curiel, Cesar Rivas-Santiago
Tobacco consumption increases the susceptibility to develop infectious diseases such as tuberculosis (TB). Nicotine (Nc) is the main component of cigarette smoke with immunomodulatory properties, however, its effect on Mycobacterium tuberculosis (Mtb) has been scarcely investigated. The present study evaluated the effect of nicotine on the growth of Mtb and on the induction of virulence-related genes
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Huoxue Jiedu Recipe represses mitochondrial fission to alleviate submandibular gland inflammation in Sjögren's syndrome Microbiol. Immunol. (IF 2.6) Pub Date : 2023-06-20 Jiangxi Xu, Zhengdong Shen, Yun Du, Shai Zhang, Yajing Xi, Yuting Zhang, Dan Wang, Yuelan Zhu
Sjögren's syndrome (SS) is the second most common autoimmune rheumatism. Huoxue Jiedu Recipe (HXJDR) is a kind of traditional Chinese medicine with a variety of pharmacological functions; however, its biological function in SS has not been studied yet. Peripheral blood mononuclear cells (PBMCs) and serum samples were isolated from healthy controls and patients with SS. NOD/Ltj mice were used for developing
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Re-recognition of innate immune memory as an integrated multidimensional concept Microbiol. Immunol. (IF 2.6) Pub Date : 2023-06-13 Chang Tang, Shoichiro Kurata, Naoyuki Fuse
In the past decade, the concept of immunological memory, which has long been considered a phenomenon observed in the adaptive immunity of vertebrates, has been extended to the innate immune system of various organisms. This de novo immunological memory is mainly called “innate immune memory”, “immune priming”, or “trained immunity” and has received increased attention because of its potential for clinical
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Capture and neutralization of SARS-CoV-2 and influenza virus by algae-derived lectins with high-mannose and core fucose specificities Microbiol. Immunol. (IF 2.6) Pub Date : 2023-05-29 Tanuza Nazmul, Basirat M. Lawal-Ayinde, Tomoko Morita, Reiko Yoshimoto, Akifumi Higashiura, Akima Yamamoto, Toshihito Nomura, Yukiko Nakano, Makoto Hirayama, Hiroshi Kurokawa, Yasuyuki Kitamura, Kanji Hori, Takemasa Sakaguchi
We first investigated the interactions between several algae-derived lectins and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). We created lectin columns using high-mannose (HM)–type glycan-specific lectins OAA and KAA-1 or core fucose–specific lectin hypninA-2 and conducted binding experiments with SARS-CoV-2. The results showed that these lectins were capable of binding to the virus
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Involvement of ribonuclease Y in pilus production by M49 Streptococcus pyogenes strain via modulation of messenger RNA level of transcriptional regulator Microbiol. Immunol. (IF 2.6) Pub Date : 2023-05-03 Seiko Kubota, Masanobu Nakata, Yujiro Hirose, Masaya Yamaguchi, Bernd Kreikemeyer, Narikazu Uzawa, Tomoko Sumitomo, Shigetada Kawabata
Streptococcus pyogenes displays a wide variety of pili, which is largely dependent on serotype. A distinct subset of S. pyogenes strains that possess the Nra transcriptional regulator demonstrates thermoregulated pilus production. Findings obtained in the present study of an Nra-positive serotype M49 strain revealed involvement of conserved virulence factor A (CvfA), also referred to as ribonuclease
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A basic charge cluster near the C-terminus of the cytoplasmic tail contributes to the molecular stability of human herpesvirus 8 E3 ubiquitin ligases Microbiol. Immunol. (IF 2.6) Pub Date : 2023-04-17 Mizuho Kajikawa, Koya Kato, Hayato Takahashi, Seiya Kitajima, Yosuke Kusunoki, Mizuki Haga, Minako Kimura, Yoshihiro Inoue, Kei Miyano, Taisei Kanamoto
Human herpesvirus 8 (HHV8; also known as Kaposi's sarcoma-associated herpesvirus [KSHV]) utilizes the viral E3 ubiquitin ligase family members K3 and K5 for immune evasion. Both K3 and K5 mediate the ubiquitination of host MHC class I (MHC-I) molecules, which play a key role in antigen presentation to cytotoxic T lymphocytes (CTLs). Because ubiquitinated MHC-I is immediately down-regulated from the
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Phylogenic position and low genomic diversity of “Candidatus Rickettsia kotlanii” inferred by complete genome sequences of two Japanese isolates Microbiol. Immunol. (IF 2.6) Pub Date : 2023-03-30 Yasuhiro Gotoh, Ai Takano, Masako Andoh, Kentaro Kasama, Keiji Nakamura, Shuji Ando, Tetsuya Hayashi
Many Rickettsia species of the spotted fever group (SFG) cause tick-borne diseases known as “spotted fever.” One of the candidate SFG Rickettsia species is “Candidatus Rickettsia kotlanii,” which was first detected in Haemaphysalis concinna in Hungary in 2006. However, its precise phylogenetic position in the SFG is not clear because only single-gene sequence–based phylogenetic analyses were performed
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Immunization with autotransporter Vag8 prevents coughing induced by Bordetella pertussis infection in mice Microbiol. Immunol. (IF 2.6) Pub Date : 2023-03-28 Koichiro Suzuki, Yukihiro Hiramatsu, Takashi Nishida, Yasuhiko Horiguchi
Bordetella pertussis causes pertussis, which is characterized by paroxysmal coughing. This disease is generally prevented through vaccination; however, the number of pertussis cases is increasing worldwide despite high vaccination coverage. We previously reported that an autotransporter of B. pertussis, virulence-associated gene 8 (Vag8), causes coughing in combination with pertussis toxin and lipooligosaccharide
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Predictive value of CXCL10 for the occurrence of immune-related adverse events in patient with renal cell carcinoma Microbiol. Immunol. (IF 2.6) Pub Date : 2023-03-28 Yuji Miura, Takanobu Motoshima, Toshiki Anami, Hiromu Yano, Remi Mito, Cheng Pan, Shinji Urakami, Keiichi Kinowaki, Hirotake Tsukamoto, Ryoma Kurahashi, Yoji Murakami, Junji Yatsuda, Yukio Fujiwara, Tomomi Kamba, Yoshihiro Komohara
Immune checkpoint inhibitors (ICIs) have recently improved the prognosis of various cancers. By contrast, some immune-related adverse events (irAEs) caused by ICIs are fatal and have become problematic. The pathogenesis of irAEs remains unknown and must be elucidated to establish biomarkers. This study investigated plasma cytokine, chemokine, and anti-CD74 autoantibody levels in patients with renal
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Human cytomegalovirus infection activates NLRP3 inflammasome by releasing mtDNA into the cytosol in human THP-1 cells Microbiol. Immunol. (IF 2.6) Pub Date : 2023-03-18 Xi Xu, Junwen Cai, Xiaoming Wang, Yutian Lu, Binhan Guo, Meimei Lai, Linhua Lan, Ying Peng, Xiaoqun Zheng
Human cytomegalovirus (HCMV) infection of monocytes results in the production of inflammatory cytokine through inflammasome. However, the mechanism of NLR family pyrin domain containing 3 (NLRP3) inflammasome activation in HCMV infection remains unclear. In this study, HCMV infection promoted the increase of mitochondrial fusion and caused mitochondrial dysfunction in THP-1 cells, including excessive
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Amentoflavone inhibits hepatitis B virus infection via the suppression of preS1 binding to host cells Microbiol. Immunol. (IF 2.6) Pub Date : 2023-03-16 Chie Aoki-Utsubo, Puguh Indrasetiawan, Kento Fukano, Masamichi Muramatsu, Nina Artanti, Muhammad Hanafi, Hak Hotta, Masanori Kameoka
Hepatitis B virus (HBV) is a leading cause of chronic hepatitis, liver cirrhosis, and hepatocellular carcinoma. Current therapeutic drugs for chronic HBV infection use IFN and nucleos(t)ide analogs; however, their efficacy is limited. Thus, there is an urgent need to develop new antivirals for HBV therapy. In this study, we identified a plant-derived polyphenolic bioflavonoid, amentoflavone, as a new
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Nicotine- and tar-removed cigarette smoke extract modulates the antigen presentation function of mouse bone marrow-derived dendritic cells Microbiol. Immunol. (IF 2.6) Pub Date : 2023-03-09 Kazuyuki Furuta, Takehiro Yoshioka, Kana Nishikaze, Noriko Yoshikawa, Kazuki Nakamura, Chikara Kaito
Dendritic cells (DCs) take up antigens derived from pathogens such as bacteria and viruses, and from tumor cells and induce the activation of antigen-specific T cells through major histocompatibility complex (MHC)-mediated antigen presentation. Mainstream cigarette smoke extract (CSE) has various effects, and the effects of its major components, nicotine and tar, have been analyzed extensively. Recently
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Recent advances in studies on magnetosome-associated proteins composing the bacterial geomagnetic sensor organelle Microbiol. Immunol. (IF 2.6) Pub Date : 2023-03-09 Azuma Taoka, Yukako Eguchi, Rino Shimoshige, Yoshihiro Fukumori
Magnetotactic bacteria (MTB) generate a membrane-enclosed subcellular compartment called magnetosome, which contains a biomineralized magnetite or greigite crystal, an inner membrane–derived lipid bilayer membrane, and a set of specifically targeted associated proteins. Magnetosomes are formed by a group of magnetosome-associated proteins encoded in a genomic region called magnetosome island. Magnetosomes