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Repurposing rabeprazole sodium as an anti-Clostridium perfringens drug by inhibiting perfringolysin O. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-12-01 Guizhen Wang,Yan Liu,Le Deng,Hongtao Liu,Xuming Deng,Quanshun Li,Haihua Feng,Zhimin Guo,Jiazhang Qiu
AIMS Clostridium perfringens infections affect food safety, human health, and the development of the poultry feed industry. Anti-virulence is an alternative strategy to develop new drug. Perfringolysin O (PFO) is an exotoxin of C. perfringens that has been demonstrated to play critical roles in the pathogenesis of this organism, promising it an attractive target to explore drugs to combat C. perfringens
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High prevalence of Panton-Valentine Leucocidin among Staphylococcus coagulans isolated from dogs in Rio de Janeiro. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-12-01 Izabel M Teixeira,Luciana Guimarães,Isabella T da Silva,Carolina Fonseca,Yasmim Assumpção,Ana Luiza Lima Dos Santos,Milena Antunes,Camilla Pesset,Eliane Ferreira,Bruno Penna
AIMS The purpose of this study was to characterize the capacity for biofilm formation, antimicrobial resistance rates, and search for genetic determinants of resistance and virulence in the species. METHODS AND RESULTS Strains were collected from asymptomatic and infected dogs. Identification was conducted using matrix-assisted laser desorption ionization-time of flight (MALDI-TOF), antimicrobial susceptibility
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In vitro inhibition of Sclerotinia sclerotiorum mycelial growth and reduction of sclerotial viability by the volatile bioactive compounds of Brassicaceae crops. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-29 Madhavi P Dassanayaka,Seona G Casonato,E Eirian Jones
AIMS Sclerotinia sclerotiorum is an important pathogen of a wide range of crops, with current control mostly relying on the use of fungicides. This study assessed the effect of biofumigation on in vitro inhibition of mycelial growth and reduction of sclerotial viability of Sclerotinia sclerotiorum as an attempt to seek an alternative management strategy. METHODS AND RESULTS The effect of different
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Antimicrobial potential of secondary metabolites and DNA gyrase B blocking molecules produced by a halophilic bacterium Virgibacillus salarius (MML1918). J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-29 Manivannan Nandhagopal,Ramprasath Chandrasekaran,Mathivanan Narayanasamy
AIM The present study aims to determine the antimicrobial potential of V. salairus (MML1918) against human pathogens and its In-vitro and In-silico properties. METHODS AND RESULTS In this present study totally 63 halophilic bacterial cultures were obtained and cultivated in nutrient broth medium containing 8% NaCl and the metabolites, were extracted using ethyl acetate and screened for their antimicrobial
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Identification and characterization of motile Aeromonas spp. isolated from farmed Nile tilapia (Oreochromis niloticus) in the Philippines. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-27 Francis S Legario,Casiano H Choresca,Kathryn Grace,James F Turnbull,Margaret Crumlish
AIMS Motile aeromonas septicaemia (MAS) caused by motile Aeromonas species is an important disease in farmed freshwater fish due to intensification of culture and improper farm practices. This study characterized and profiled motile Aeromonas species recovered from clinically sick tilapia farmed in the Philippines, with a view to identifying targeted disease prevention and control measures against
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Comparison of databases useful for the analysis of vaginal microbiota in Japanese women using next-generation sequencing data (QIIME 2 software). J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-27 Xianya Zou,Yukiko Nakura,Haruna Kawaguchi,Fumiko Nishiumi,Heng Ning Wu,Itaru Yanagihara
AIMS Approximately 10% of children are born prematurely, and bacterial vaginosis during pregnancy is associated with preterm delivery. Highly accurate species-level vaginal microflora analysis helps control bacteria-induced preterm birth. Therefore, we aimed to conduct a bioinformatic analysis of gene sequences using 16S databases and compare their efficacy in comprehensively identifying potentially
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Culturing the unculturables: strategies, challenges, and opportunities for gut microbiome study. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-24 Asha Yadav,Shruti Ahlawat,Krishna Kant Sharma
Metagenome sequencing techniques revolutionized the field of gut microbiome study. However, it is equipped with experimental and computational biases which affect the downstream analysis results. Also, live microbial strains is needed for a better understanding of host-microbial crosstalks and for designing next-generation treatment therapies based on probiotic strains and postbiotic molecules. Conventional
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Evaluation of Lentilactobacillus parafarraginis A6-2 Strain for Aluminum Removal and Anti-inflammatory Effects: implications for Alleviating Al Toxicity. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-21 Se-Won Lee,Jeong-Muk Lim,Gwang-Min Lee,Jung-Hee Park,Kamala-Kannan Seralathan,Byung-Taek Oh
AIM To assess the effectiveness of Lentilactobacillus parafarraginis A6-2 cell lysate for the removal of aluminum (Al), which induces neurotoxicity, and its protective effect at cellular level. METHODS AND RESULTS The cell lysate of the selected L. parafarraginis A6-2 strain demonstrated superior Al removal compared to live or dead cells. The Al removal efficiency of L. parafarraginis A6-2 cell lysate
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The detection of Japanese Encephalitis Virus in municipal wastewater during an acute disease outbreak. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-17 Stella Fanok,Paul Terence Monis,Alexandra Ruth Keegan,Brendon James King
AIM To demonstrate the capability of wastewater-based surveillance (WBS) as a tool for detecting potential cases of Japanese Encephalitis Virus (JEV) infection in the community. METHODS AND RESULTS In this study, we explore the potential of WBS to detect cases of JEV infection by leveraging from an established SARS-CoV-2 wastewater surveillance program. We describe the use of two reverse transcriptase
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Planktonic and Biofilm states of Staphylococcus aureus isolated from bone and joint infections and the in vitro effect of orally available antibiotics. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-17 Eduardo Perez-Alba,Samantha Flores-Treviño,Verónica Villarreal-Salazar,Paola Bocanegra-Ibarias,Félix Vilchez-Cavazos,Adrián Camacho-Ortiz
AIMS To demonstrate the in vitro activity of orally available antibiotics against Staphylococcus aureus isolated from bone or orthopedic implant materials. The biofilm eradication of the combination of three antibiotics was also assessed. METHODS AND RESULTS Clinical isolates from orthopedic infection samples were collected, and S. aureus isolates were classified according to their biofilm production
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Research on the Anti-aging efficacy of fermented lysate VHProbi® mix a as the functional skincare ingredient. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-17 Hongchang Cui,Congrui Feng,Chaoqun Guo,Xinping B,Xinping Li,Tao Zhang,Sidao Cheng,Zhi Duan
AIMS This study aimed to investigate the efficacy of a cream containing VHProbi® Mix A for improving skin aging. METHODS AND RESULTS In vitro studies demonstrated that the lysate produced from Lacticaseibacillus paracasei E12 (E12) exhibited immunoregulatory effects in a three-dimensional (3D) skin model, with significant reductions in levels of interleukin (IL)-1α, IL-1β, and IL-8 (P < 0.05) compared
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Pyrogallol impairs staphylococcal biofilm formation via induction of bacterial oxidative stress. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-16 Katharina H C Roese,Christina Torlone,Lauren A Cooper,Lee Esposito,Amy M Deveau,Ursula S R Röse,Kristin M Burkholder
AIMS To examine the effect of the phenolic compound pyrogallol on staphylococcal biofilm formation. METHODS AND RESULTS In crystal violet biofilm assays, pyrogallol reduced biofilm formation in S. epidermidis ATCC 35984, S. epidermidis NRRL-B41021, S. aureus USA300 and S. aureus Newman, without significantly impairing bacterial viability. Pyrogallol-mediated impairment of biofilm formation was likely
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Inhibitor activity of lactiplantibacillus plantarum LP5 on thermotolerant campylobacter with different biofilm-forming capacities. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-16 María Julia Ruiz,Noelí Estefanía Sirini,María Ángeles Stegmayer,Lorena Paola Soto,María Virginia Zbrun,Carolina Raquel Olivero,María Laura Werning,Federico Francisco Acosta,Marcelo Lisandro Signorini,Laureano Sebastián Frizzo
AIMS To evaluate the biofilm-forming capacity of thermotolerant Campylobacter (TC) strains from poultry production and to analyse the inhibitory capacity of Lactiplantibacillus plantarum LP5 against TC on different materials. METHODS AND RESULTS Biofilm-forming capacity by Campylobacter jejuni and Campylobacter coli was analysed by cell adhesion in polystyrene plates. TC were classified as non-biofilm-forming
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Fungal composition, quantification of mycotoxins and enzyme activity in processed Solanum tuberosum Linn (Potato) products stored at different relative humidity. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-15 Margaret M A Danladi,Chike I C Ogbonna,Abigail I Ogbonna,Cindy Giles,Mary T Fletcher,Olufemi A Akinsanmi
AIM Postharvest loss of potatoes at the peak of harvest is of global concern. This study aimed to determine the quality of stored processed potato products based on fungal composition, mycotoxin contamination and fungal enzyme activity. MATERIALS AND METHODS Potato products from three cultivars (Caruso, Marabel and Nicola) were grouped as peeled or unpeeled, oven- or sun-dried and all samples were
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Inhibitory effect of tannic acid on the growth of Apiospora arundinis and 3-Nitropropionic acid production. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-13 Wenyan Jiang,Xuelian Liang,Huiling Li,Leixing Mo,Wei Chen,Tianshun Wang,Haijun Wang,Yihao Xing,Jie Liao
AIMS This study aimed to investigate the inhibitory effect of tannic acid (TA) on the growth of Apiospora arundinis and 3-Nitropropionic acid (3-NPA) production. METHODS AND RESULTS To investigate the antifungal mechanism, the effects of TA on the hypha growth, electrical conductivity, hypha morphology, defence-related enzymes and 3-NPA production of A. arundinis were studied. TA concentrations of
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Clonal spread of non-O157 Shiga toxigenic Escherichia coli O21:H25 in raw water buffalo milks. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Tolga Uyanik,Ali Gücükoğlu,Hande Gürler,Sibel Kanat,Ayşegül Bölükbaş,Özgür Çadirci
AIMS This study was conducted to investigate the presence of Shiga toxin-producing O157 and non-O157 E. coli in raw water buffalo milk, as well as to determine the virulence gene profiles, phylogroups, sequence types, and serotypes of the isolated strains. METHODS AND RESULTS A total of 200 hand-milked raw water buffalo milk samples were collected from 200 different water buffaloes over a period of
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Berberine inhibits Candida albicans growth by disrupting mitochondrial function through the reduction of iron absorption. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Dongming Zheng,Daifan Yue,Jinyang Shen,Dongmei Li,Zhen Song,Yifu Huang,Jiangyan Yong,Yan Li
AIMS This study aimed to investigate whether berberine (BBR) can inhibit the iron reduction mechanism of Candida albicans, lowering the iron uptake of the yeast and perhaps having antimicrobial effects. METHODS AND RESULTS We determined that BBR may cause extensive transcriptional remodeling in C. albicans and that iron permease Ftr1 played a crucial role in this process through eukaryotic transcriptome
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Astragalus additive in feed improved serum immune function, rumen fermentation and the microbiota structure of early-weaned lambs. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Pengyang Shao,Yuzhu Sha,Xiu Liu,Yanyu He,Xinyu Guo,Jiang Hu,Jiqing Wang,Shaobin Li,Caiye Zhu,Guoshun Chen,Wenhao Li
AIM The purpose of this study was to determine the mechanism of Astragalus activity on the immune function, rumen microbiota structure, and rumen fermentation of early-weaned lambs. METHODS AND RESULTS Thirty healthy early-weaned lambs with similar body weights (17.42 ± 2.02 kg) were selected for the feeding experiment. The control group (KB) was fed a basal diet, and the Astragalus group (HQ) was
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Inactivation of Salmonella Typhimurium and Listeria monocytogenes on buckwheat seeds through combination treatment with plasma, vacuum packaging, and hot water. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Ye-Jin Park,Su-Yeon Kim,Won-Jae Song
AIMS The objectives of this study were to evaluate the effect of combination treatment with cold plasma (CP), vacuum packaging (VP), and hot water (HW) on the inactivation of foodborne pathogens on buckwheat seeds, and determined the germination rates of seeds and the quality of sprouts following combination treatment. METHODS AND RESULTS Buckwheat seeds inoculated with Salmonella Typhimurium and Listeria
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Glycoside hydrolase family 32 enzymes from Bombella spp. catalyze the formation of high-molecular weight fructans from sucrose. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Luca Härer,Luise Ernst,Julia Bechtner,Daniel Wefers,Matthias A Ehrmann
AIMS Acetic acid bacteria of the genus Bombella have not been reported to produce exopolysaccharides (EPS). In this study, the formation of fructans by B. apis TMW 2.1884 and B. mellum TMW 2.1889 was investigated. METHODS AND RESULTS Out of eight strains from four different Bombella species, only B. apis TMW 2.1884 and B. mellum TMW 2.1889 showed EPS formation with 50 g l-1 sucrose as substrate. Both
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Dominance of ST131, B2, blaCTX-M-15, and papA-papC-kpsMII-uitA among ESBL Escherichia coli isolated from bloodstream infections in Quito, Ecuador: a 10-year surveillance study (2009-2019). J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Jeannete Zurita,Gabriela Sevillano,Ariane Paz Y Miño,Nathalí Haro,Marco Larrea-Álvarez,Iliana Alcocer,David Ortega-Paredes
AIMS This study aimed to examine antibiotic resistance and the epidemiology of extended-spectrum β-lactamases (ESBL)-producing Escherichia coli associated with bloodstream infections over a period of 10 years. METHODS AND RESULTS Isolates were collected from January 2009 to December 2019 and those testing for E. coli were included. Antibiotic susceptibility was tested using the VITEK® system. Selected
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Enteropathogenic Escherichia coli is a predominant pathotype in healthy pigs in Hubei Province of China. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Wenxiao Zhuo,Yang Zhao,Xianglin Zhao,Zhiming Yao,Xiuxiu Qiu,Yaxue Huang,Huaixia Li,Jing Shen,Zhihao Zhu,Tingting Li,Shaowen Li,Qi Huang,Rui Zhou
AIM This study aims to investigate the prevalence of intestinal pathogenic Escherichia coli (InPEC) in healthy pig-related samples and evaluate the potential virulence of the InPEC strains. METHODS AND RESULTS A multiplex PCR method was established to identify different pathotypes of InPEC. A total of 800 rectal swab samples and 296 pork samples were collected from pig farms and slaughterhouses in
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Characterization of exogenous lactate addition on the growth, photosynthetic performance, and biochemical composition of four bait microalgae strains. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Jiawen Duan,Chenhui Li,Yimeng Zheng,Aiyou Huang,Zhenyu Xie
AIMS To quickly obtain the biomass of bait microalgae with high value-added products, researchers have examined the influence of biochemical and environmental factors on the growth rates and biochemical composition of microalgae. Previous studies have shown that lactate plays an important role in metabolic regulation in Phaeodactylum tricornutum. In this study, we investigated the effect of exogenous
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Correction to: Physical characterisation of Tenacibaculum maritimum for vaccine development. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01
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In-vitro polymicrobial oral biofilm model represents clinical microbial profile and disease progression during implant-related infections. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Caroline Dini,Raphael Cavalcante Costa,Martinna Bertolini,Jamil Awad Shibli,Magda Feres,Marlise Inêz Klein,Érica Dorigatti de Avila,João Gabriel Silva Souza,Valentim Adelino Ricardo Barão
AIM Clinically relevant in-vitro biofilm models are essential and valuable tools for mechanistically dissecting the etiopathogenesis of infectious diseases and test new antimicrobial therapies. Thus, the aim of this study was to develop and test a clinically relevant in-vitro oral polymicrobial biofilm model that mimics implant-related infections in terms of microbial profile. METHODS AND RESULTS For
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Structural characteristics and functional properties of a fucose containing prebiotic exopolysaccharide from Bifidobacterium breve NCIM 5671. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Rwivoo Baruah,P Pramod Kumar,Surabhi Gangani,K V Harish Prashanth,Prakash M Halami
AIM To evaluate the structure and functions of capsular exopolysaccharide (CPS) from Bifidobacterium breve NCIM 5671. METHODS AND RESULTS A CPS produced by the probiotic bacteria B. breve NCIM 5671 was isolated and subjected to characterization through GC analysis, which indicated the presence of rhamnose, fucose, galactose, and glucose in a molar ratio of 3:1:5:3. The average molecular weight of the
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Antioxidant effect of lactic acid bacteria in human bronchial epithelial cells exposed to cigarette smoke. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Mirjana Babic,Katarina Veljovic,Nikola Popović,Natasa Golic,Dragica Radojkovic,Marija Stankovic
AIMS Chronic lung diseases are a major and increasing global health problem, commonly caused by cigarette smoke. We aimed to explore the antioxidant effects of lactic acid bacteria (LAB) against cigarette smoke in bronchial epithelial cells. METHODS AND RESULTS The antioxidant effects of 21 heat-killed (HK) LAB strains were tested in cigarette smoke-stimulated BEAS-2B cells and 3-D bronchospheres organoids
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A multiplex real-time RT-PCR system to simultaneously diagnose 16 pathogens associated with swine respiratory disease. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Yusuke Goto,Kazuhiro Fukunari,Shigekatsu Tada,Satoki Ichimura,Yuzumi Chiba,Tohru Suzuki
AIMS Swine respiratory disease (SRD) is a major disease complex in pigs that causes severe economic losses. SRD is associated with several intrinsic and extrinsic factors such as host health status, viruses, bacteria, and environmental factors. Particularly, it is known that many pathogens are associated with SRD to date, but most of the test to detect those pathogens can be normally investigated only
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Isolation, characterization of Enterococcus phages and their application in control of E. faecalis in milk. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Chen Wang,Junxin Zhao,Yunzhi Lin,Lu Yuan,Mohamed El-Telbany,Aye Thida Maung,Marwa Nabil Sayed Abdelaziz,Yoshimitsu Masuda,Ken-Ichi Honjoh,Takahisa Miyamoto
AIMS Isolation and characterization of Enterococcus phages and application of phage cocktail to control E. faecalis in milk. METHODS AND RESULTS For phage isolations, double layer agar method was used. Host range of the phages were determined by the spot test. Twelve phages with varying host ranges were isolated. Phages PEF1, PEF7b, and PEF9 with different host ranges and lytic activities were selected
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Composition, functional, and niche differentiation of fungal communities in the rhizosphere and root endosphere of Cinnamomum migao in southwestern China. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Lan Zhang,Xiaolong Huang,Bingli Tong,Meng Chen,Luting Huang,Jiming Liu
AIMS Roots are key parts of plant material circulation and energy flow, creating two distinct niches for the plant microbiome, such as the rhizosphere and root endosphere, which interact to maintain plant growth and health. In this study, two niches of plant rhizosphere and root endosphere were selected to study the composition and differences of fungi communities in order to better understand how
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Physiological and genomic characterization of Lactiplantibacillus plantarum isolated from Indri indri in Madagascar. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Nanzhen Qiao,Gautam Gaur,Monica Modesto,Fabio Chinnici,Donatella Scarafile,Luigimaria Borruso,Antonio Castro Marin,Caterina Spiezio,Daria Valente,Camillo Sandri,Michael G Gänzle,Paola Mattarelli
AIMS Indri indri is a lemur of Madagascar which is critically endangered. The analysis of the microbial ecology of the intestine offers tools to improve conservation efforts. This study aimed to achieve a functional genomic analysis of three Lactiplantibacillus plantarum isolates from indris. METHODS AND RESULTS Samples were obtained from 18 indri; 3 isolates of Lp. plantarum were obtained from two
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Alcohol degradation, learning, and memory-enhancing effect of Acetobacter pasteurianus BP2201 in Caenorhabditis elegans model. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Xin Wen,Huazhong Yang,Zhongqi Li,Weihua Chu
AIMS This study aimed to investigate the probiotic effects of Acetobacter pasteurianus BP2201, isolated from brewing mass, for the treatment of alcohol-induced learning and memory ability impairments in a Caenorhabditis elegans model. METHODS AND RESULTS Acetobacter pasteurianus BP2201 was examined for probiotic properties, including acid and bile salt resistance, ethanol degradation, antioxidant efficacy
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Rapid AMR prediction in Pseudomonas aeruginosa combining MALDI-TOF MS with DNN model. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Jiaojiao Fu,Fangting He,Jinming Xiao,Zhengyue Liao,Liying He,Jing He,Jinlin Guo,Sijing Liu
BACKGROUND Pseudomonas aeruginosa is a significant clinical pathogen that poses a substantial threat due to its extensive drug resistance. The rapid and precise identification of this resistance is crucial for effective clinical treatment. Although matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used for antibiotic susceptibility differentiation
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Characterization of potential probiotic starter cultures of lactic acid bacteria isolated from Ethiopian fermented cereal beverages, Naaqe, and Cheka. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Seyoum Gizachew,Wannes Van Beeck,Irina Spacova,Max Dekeukeleire,Ashenafi Alemu,Wude Mihret,Sarah Lebeer,Ephrem Engidawork
AIMS To test the in vitro probiotic potential and starter culture capacity of lactic acid bacteria (LAB) isolated from Naaqe and Cheka, cereal-based Ethiopian traditional fermented beverages. METHODS AND RESULTS A total of 44 strains were isolated from spontaneously fermented Ethiopian cereal-based beverages, Naaqe and Cheka with 24 putatively identified as LAB and 14 identified up to the species level
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Bifidobacterium longum subsp. longum BL21 ameliorates alcoholic liver disease in mice through enhancement of the hepatic antioxidant capacity and modulation of the gut microbiota. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Yao Dong,Zhiyi Wu,Zhonghui Gai,Mei Han
BACKGROUND Alcoholic liver disease (ALD) is a chronic liver injury caused by excessive alcohol consumption, could be impacted by gut-liver axis dysfunction. The gut microbiota plays a crucial role in the development and progression of ALD. Given the role of gut-liver axis dysfunction in ALD, strategies targeting gut microbiota modulation have gained interest for therapeutic interventions. Bifidobacterium
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MALDI-TOF bacterial subtyping for rapid detection of biomarkers in Staphylococcus aureus from subclinical bovine mastitis. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Thainara Lopes,Carlos E Fidelis,Amanda T F Silva,Rinaldo A Mota,Vera L M Rall,Marcos V Dos Santos,Juliano L Gonçalves
AIMS This study aimed to evaluate matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial subtyping for the rapid detection of biomarkers in Staphylococcus aureus from subclinical bovine mastitis. METHODS AND RESULTS A total of 229 S. aureus isolates were obtained from milk samples collected from cows with subclinical mastitis using microbiological culture
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Assessment of soil yeasts Papiliotrema laurentii S-08 and Saitozyma podzolica S-77 for plant growth promotion and biocontrol of Fusarium wilt of brinjal. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Sukanya Das,Jintu Rabha,Diganta Narzary
AIM This study aimed to determine the efficacy of the soil yeasts Papiliotrema laurentii S-08 and Saitozyma podzolica S-77 for plant growth promotion (PGP) and biocontrol of wilt disease in brinjal plants while applying yeasts individually or as a consortium in pot experiments. METHODS AND RESULTS The yeasts were tested for various PGP characteristics and antagonistic activity against the phytopathogen
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Butyl succinate-mediated control of Bacillus velezensis ce 100 for apple anthracnose caused by Colletotrichum gloeosporioides. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Seo Hyun Hwang,Chaw Ei Htwe Maung,Jun Su Noh,Jeong-Yong Cho,Kil Yong Kim
AIMS Microbial biocontrol agents have become an effective option to mitigate the harmfulness of chemical pesticides in recent years. This study demonstrates the control efficacy of Bacillus velezensis CE 100 on the anthracnose causal agent, Colletotrichum gloeosporioides. METHODS AND RESULTS In vitro antifungal assays revealed that the culture filtrate and volatile organic compounds of B. velezensis
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Synergistic antifungal activity and potential mechanism of action of a glycolipid-like compound produced by Streptomyces blastmyceticus S108 against Candida clinical isolates. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 A Ayed,R Essid,H Mankai,A Echmar,N Fares,M Hammami,N Sewald,F Limam,O Tabbene
AIM The present study aimed to investigate a novel antifungal compound produced by Streptomyces blastmyceticus S108 strain. Its effectiveness against clinical isolates of Candida species and its synergistic effect with conventional antifungal drugs were assessed, and its molecular mechanism of action was further studied against Candida albicans. METHODS AND RESULTS A newly isolated strain from Tunisian
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Survey on microbial contamination of opened skin care products used for tattooing. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Szilvia Neuhaus,Stefan Brockmann,Sascha Al Dahouk,Ralf Dieckmann
AIMS Inadequate hygiene measures as well as the use of contaminated inks or non-sterile needles are considered as important infection sources in the process of tattooing. In tattoo parlors and at conventions, it is common practice to apply cosmetic products from bulk packs as lubricants during tattooing and as ointments for tattoo aftercare. The objective of our study was to assess the microbial load
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Determining the efficacy of disinfectants at nucleic acid degradation. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Rachael C Wilkinson,Kirsty Meldrum,Caitlin J Maggs,Nerissa E Thomas,Bethan R Thomas,Natalie De Mello,Naomi Joyce
AIMS Nucleic acids, particularly antibiotic resistance genes, are commonly found on surfaces within healthcare environments, with levels not reducing following cleaning. Within the UK, there are no regulations for testing disinfectants against nucleic acids. METHODS AND RESULTS A series of commonplace in vitro methods were used to determine disinfectant-induced physical and functional damage to various
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Antifungal activity of Klebsiella grimontii DR11 against Fusarium oxysporum causing soybean root rot. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Shengyi Wang,Lining Zheng,Ao Gao,Yufeng Xiao,Zhe Han,Hongyu Pan,Hao Zhang
AIMS Soybean root rot, caused by Fusarium oxysporum, leads to significant economic and financial losses to the soybean processing industry globally. In the study, we aimed to explore a biocontrol agent to combat F. oxysporum infection in soybean. METHODS AND RESULTS From soybean rhizosphere soil, 48 strains were isolated. Among them, the strain DR11 exhibited the highest inhibition rate of 72.27%.
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The relationship between gut microbiota, short-chain fatty acids, and glucolipid metabolism in pregnant women with large for gestational age infants. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Yehui Lan,Shuangjia Pan,Baoyi Chen,Feifei Zhou,Fan Yang,Shan Chao,Ying Hua,Haibin Liu
AIM To elucidate the association between gut microbiota, short-chain fatty acids (SCFAs), and glucolipid metabolism in women with large for gestational age (LGA) infants. METHODS AND RESULTS A single-center, observational prospective cohort study was performed at a tertiary hospital in Wenzhou, China. Normal pregnant women were divided into LGA group and appropriate for gestational age (AGA) group
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Evaluating the incidence of ampC-β-lactamase genes, biofilm formation, and antibiotic resistance among hypervirulent and classical Klebsiella pneumoniae strains. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Mohammad Hossein Haddadi,Saeed Khoshnood,Maryam Koupaei,Mohsen Heidary,Melika Moradi,Ali Jamshidi,Hamid Behrouj,Ahmad Movahedpour,Mohammad Hassan Maleki,Roya Ghanavati
AIM Both immunocompetent and healthy individuals can become life-threateningly ill when exposed to the hypervirulent (hvKp) strains of Klebsiella pneumoniae (Kp). The main objectives of this study were to evaluate the presence of ampC-lactamase genes, biofilm formation, and antibiotic resistance in clinical strains of hvKp and cKp (classical K. pneumoniae). MATERIALS AND METHODS Kp strains were collected
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Occurrence of high-risk clones of Klebsiella pneumoniae ST11, ST340, and ST855 carrying the blaKPC-2, blaNDM-1, blaNDM-5, and blaNDM-7 genes from colonized and infected patients in Brazil. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 Érica Maria de Oliveira,Elizabeth Maria Bispo Beltrão,Maria Izabely Silva Pimentel,Ana Catarina de Souza Lopes
AIMS Determine which sequence type (ST) clones were carrying the blaKPC, blaNDM, blaVIM, blaIMP, and blaGES genes and their variants in clinical isolates of multidrug-resistant Klebsiella pneumoniae. METHODS AND RESULTS Ten K. pneumoniae isolates were obtained from the colonized and infected patients in a public hospital in the city of Recife-PE, in northeastern Brazil, and were further analyzed. The
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New insights into peroxide toxicology: sporulenes help Bacillus subtilis endospores from hydrogen peroxide. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-11-01 N Smita,Ch Sasikala,ChV Ramana
AIM The purpose of the present study was to understand the possible events involved in the toxicity of hydrogen peroxide (H2O2) to wild and sporulene-deficient spores of Bacillus subtilis, as H2O2 was previously shown to have deleterious effects. METHODS AND RESULTS The investigation utilized two strains of B. subtilis, namely the wild-type PY79 (WT) and the sporulene-deficient TB10 (ΔsqhC mutant)
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Gallic acid inhibits Staphylococcus aureus RecA protein functions: role in countering antibiotic resistance in bacteria. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-06 Kajal Kiran,K Neelakanteshwar Patil
AIM Recombinase RecA and its homologs play a key role in homologous recombination DNA repair and revive stalled replication fork DNA synthesis. RecA plays an essential role in the evolution of antibiotic-resistant strains via stress-induced DNA repair mechanisms during the SOS response. Accordingly, RecA has become an attractive target to slow down antibiotic resistance rates and prevent mutations
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Regulation of the pigment production by changing Cell morphology and gene expression of Monascus ruber in high-sugar synergistic high-salt stress fermentation. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Gong Chen,Wenqian Zhao,Lu Zhao,Da Song,Ben Chen,Xihong Zhao,Ting Hu
AIMS Extreme environment of microbial fermentation is the focus of research, which provides new thinking for the production and application of Monascus pigments (MPs). In this work, the high-sugar synergistic high-salt stress fermentation (HSSF) of MPs was investigated. METHODS AND RESULTS The Monascus fungus grew well under HSSF conditions with 35 g L-1 NaCl and 150 g L-1 glucose, and the extracellular
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Daptomycin production enhancement by ARTP mutagenesis and fermentation optimization in Streptomyces roseosporus. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Chen-Yang Zhu,Xin-Yi Zhao,Zhong-Yuan Lyu,Wen-Li Gao,Qing-Wei Zhao,Xin-Ai Chen,Yong-Quan Li
AIMS We evaluated whether the randomness of mutation breeding can be regulated through a double-reporter system. We hope that by establishing a new precursor feeding strategy, the production capacity of industrial microorganisms after pilot scale-up can be further improved. METHODS AND RESULTS In this study, the industrial strain Streptomyces roseosporus L2796 was used as the starter strain for daptomycin
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Use of Pantoea agglomerans ASB05 as a biocontrol agent to inhibit the growth of Salmonella enterica on intact cantaloupe melons. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Sang In Lee,Thao D Tran,Robert Hnasko,Jeffery A McGarvey
AIMS To identify biocontrol agents to prevent the growth of Salmonella serotype Enterica on cantaloupe melons during the pre- and postharvest periods. METHODS AND RESULTS We created a produce-associated bacterial library containing 8736 isolates and screened it using an in-vitro fluorescence inhibition assay to identify bacteria that inhibit the growth of S. Enterica. One isolate, Pantoea agglomerans
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Insights into molecular mechanism of plasticizer biodegradation in Dietzia kunjamensis IITR165 and Brucella intermedia IITR166 isolated from a solid waste dumpsite. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Saurabh Singh,Ravindra Singh Thakur,Natesan Manickam
AIMS Isolation of phthalate esters (PAEs) degrading bacteria from a solid waste dumpsite could degrade many plasticizers efficiently and to investigate their degrading kinetics, pathways, and genes. METHODS AND RESULTS Based on their 16S rRNA gene sequence the strains were identified as Dietzia kunjamensis IITR165 and Brucella intermedia IITR166, which showed a first-order degradation kinetic model
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Evaluation of phenotypical and genotypical methods for the identification and typing of Stenotrophomonas maltophilia isolated from a pharmaceutical facility. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Rebeca Vitória da Silva Lage de Miranda,Giovanna Merrelho Monteiro,Luciana Veloso da Costa,Milena Cristina Silva Dos Santos,Cristhiane Moura Falavina Dos Reis,Lygia Maria Paulo da Silva Braga,Stephen James Forsythe,Maria Helena Simões Villas Bôas,Marcelo Luiz Lima Brandão
AIMS Evaluate methods for identification and typing of Stenotrophomonas maltophilia isolated from a pharmaceutical facility. METHODS AND RESULTS From 270 S. maltophilia strains identified by VITEK®2, 40 were selected and submitted to MALDI TOF-MS, 16S and 23S rRNA gene analysis, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and an antimicrobial susceptibility
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Inhibitory effect of bilobalide on Staphylococcus aureus von Willebrand factor-binding protein and its therapeutic effect in mice with pneumonia. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Juan Hou,Yijing Jiang,Yangming Xu,Chunhui Zhao,Yali Cao,Wu Song,Bingmei Wang
AIMS Disabling bacterial virulence with small molecules has been proposed as a potential strategy to prevent bacterial pathogenicity. The von Willebrand factor-binding protein of Staphylococcus aureus was identified previously as a key virulence determinant. Our objective was to discover a von Willebrand-factor binding protein (vWbp) inhibitor distinct from the antibiotics used to prevent infections
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Hydrocinnamic acid and perillyl alcohol are effective against Escherichia coli biofilms when used alone and combined with antibiotics. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Mariana Sousa,Ana Cristina Afonso,Maria José Saavedra,Lúcia Chaves Simões,Manuel Simões
AIMS The use of phytochemicals to improve the effectiveness of antibiotics is a promising strategy for the development of novel antimicrobials. In this study, the antibiofilm activity of perillyl alcohol and hydrocinnamic acid, both phytochemicals present in several plants, and two antibiotics from different classes (amoxicillin and chloramphenicol) was tested, alone and in combination, against Escherichia
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The possibility of spreading herpes simplex virus type 1 via food handling and sharing. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Gabrielle Pageau,Marianne Levasseur,Teresa Paniconi,Eric Jubinville,Valérie Goulet-Beaulieu,Guy Boivin,Julie Jean
AIMS Herpes simplex virus type 1 (HSV-1) is an enveloped virus that causes recurrent and incurable diseases in 67% of the world population. Although it is not listed as a foodborne virus, some studies have shown that it can be recovered from surfaces as well as food. METHODS AND RESULTS We investigated its persistence at -20°C, 4°C, 20°C, or 37°C for up to 7 days on stainless steel, aluminum, glass
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Ruxolitinib accelerates influenza A virus adaptation in the Madin-Darby canine kidney (MDCK) cell line. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Hana Malenovská
AIM To investigate the effect of ruxolitinib medium supplement, separately and in combination with trypsin, on influenza A virus (IAV) adaptation and propagation in the Madin-Darby canine kidney (MDCK) cell line. METHODS AND RESULTS Two consecutive passages of three egg-based IAV strains were performed in the MDCK cell line with medium (a) without additives; (b) with a combination of ruxolitinib and
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The occurrence and genomic characteristics of the blaIMI-1 carbapenemase-producing Enterobacter cloacae complex retrieved from natural water sources in central Thailand. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Wipawee Songsaeng,Nuvee Prapasarakul,Thidathip Wongsurawat,Wandee Sirichokchatchawan
AIM Carbapenem resistance among Enterobacteriaceae is a serious threat to humans worldwide. This study aims to evaluate the phenotypic and genotypic characterization of carbapenemase-producing Enterobacter cloacae complex (ECC) retrieved from water sources in the central part of Thailand. METHODS AND RESULTS Samples were collected from water bodies surrounding farms and communities in central Thailand
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In vitro evaluation of the efficacy of 8-hydroxyquinoline derivatives for the control of Phaeomoniella chlamydospora, the causative agent of Petri disease in grapevines. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Luciana M de Souza,Angelica R Joaquim,Angelo Gava,Evandro Ficagna,Marcus A K Almança,Saulo F Andrade,Alexandre M Fuentefria
AIM This study evaluates the in vitro efficacy of 8-hydroxyquinoline (8HQ) derivatives in controlling the phytopathogenic fungus Phaeomoniella chlamydospora. METHODS AND RESULTS The in vitro tests assessed the susceptibility to the minimum inhibitory concentration (MIC), checkerboard assay, mycelial growth (MG) inhibition, and EC50 determination. Among the seven agricultural fungicides tested, tebuconazole
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Cupferron impairs the growth and virulence of Escherichia coli clinical isolates. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Francesca Palma,Federica Dell'Annunziata,Veronica Folliero,Francesco Foglia,Roberta Della Marca,Carla Zannella,Anna De Filippis,Gianluigi Franci,Massimiliano Galdiero
AIMS Multidrug resistance is a worrying problem worldwide. The lack of readily available drugs to counter nosocomial infections requires the need for new interventional strategies. Drug repurposing represents a valid alternative to using commercial molecules as antimicrobial agents in a short time and with low costs. Contextually, the present study focused on the antibacterial potential of the ammonium
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Growth of alfalfa in the presence of metabolites from a dark septate endophyte strain Alternaria sp. 17463 cultured with a nonionic surfactant and emulsifier. J. Appl. Microbiol. (IF 4.0) Pub Date : 2023-10-04 Hai Tan,Yinli Bi,Shishuang Zhang,Shuhui Wang
AIM Dark septate endophytes (DSE) were widely used in the agriculture and ecological restoration. The objective of this work was to assess the effect of culture media nonionic surfactant and emulsifier on the biomass and metabolites of DSE strain Alternaria sp. 17463. METHODS AND RESULTS Changes in the composition of DSE metabolites following the addition of Tween 80 during liquid culture of a DSE