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Septoria cannabicola, a new species on Cannabis sativa from Japan Mycoscience (IF 1.4) Pub Date : 2024-03-02 Anysia Hedy Ujat, Shinju Konishi, Yurina Kato, Hana Tonami, Chiharu Nakashima
Septoria leaf spot on hemp has re-emerged with increasing hemp cultivation worldwide. In Japan, Septoria cannabis, initially recorded as the causal pathogen in Japan, was studied with morphology based on the current criteria and detailed molecular phylogenetic analyses using seven gene loci. The robust phylogenetic data and morphology of examined specimens unveiled the existence of a new species of
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Gymnosporangium mori comb. nov. (Pucciniales) for Caeoma mori (≡ Aecidium mori) inferred from phylogenetic evidence Mycoscience (IF 1.4) Pub Date : 2024-03-02 Taiga Kasuya, Kentaro Hosaka, Jing-Xin Ji, Makoto Kakishima
Caeoma mori (≡ Aecidium mori), known as the mulberry rust which is an anamorphic rust fungus forming only aecidioid uredinia, were found on Morus alba in Ibaraki and Saitama Prefectures, Japan. Molecular phylogenetic analyses using the combined dataset of sequences from 28S and 18S of the nuclear ribosomal RNA gene and Cytochrome-c-oxidase subunit 3 of the mitochondrial DNA revealed that this anamorphic
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Re-epitypification of Neofusicoccum laricinum Mycoscience (IF 1.4) Pub Date : 2024-03-02 Yukako Hattori, Chiharu Nakashima, Hayato Masuya
The lectotype and epitype of Neofusicoccum laricinum proposed in previous studies failed to meet the requirements of the International Code of Nomenclature for Algae, Fungi, and Plants. Here, we propose a valid lectotype and epitype for this species that meet the requirements of the Code.
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Anthostomella-like fungi on bamboo: four new genera belonging to a new family Pallidoperidiaceae (Xylariales) Mycoscience (IF 1.4) Pub Date : 2024-01-30 Ryosuke Sugita, Ryuichi Yoshioka, Kazuaki Tanaka
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Geastrum sanglinense, a new species from the Manghe Rhesus Monkey National Nature Reserve, China Mycoscience (IF 1.4) Pub Date : 2024-01-30 Yanqun Wu, Shuqin Liu, Tengyun Wang, Junlong Meng, Mingchang Chang, Shurong Wang
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Cercosporamide, a polyketide-derived fungal metabolite, serves as an antifungal agent against phytopathogenic fungi Mycoscience (IF 1.4) Pub Date : 2024-01-30 Rundong Liu, Jaycee Augusto Paguirigan, Jae-Seoun Hur, Wonyong Kim
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Greetings from virologists to mycologists: A review outlining viruses that live in fungi Mycoscience (IF 1.4) Pub Date : 2024-01-30 Syun-ichi Urayama, Yan-jie Zhao, Misa Kuroki, Yuto Chiba, Akihiro Ninomiya, Daisuke Hagiwara
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Neoerysiphe leontopodii sp. nov., a new powdery mildew from China Mycoscience (IF 1.4) Pub Date : 2023-11-01 Tie-Zhi Liu, Li Liu, Jing Wen, Shu-Yan Liu
A powdery mildew was found on Leontopodium leontopodioides (Asteraceae) in China. Phylogenetic analyses using a combination of internal transcribed spacer and 28S rDNA sequences showed that this species, which clusters as sister to Neoerysiphe joerstadii, is allied to N. galii, N. geranii, and N. nevoi. This species differs from the closely allied N. joerstadii in the number and size of asci (3–10
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Cryphonectria carpinicola discovered in Japan: first report of the sexual state on Carpinus tree. Mycoscience (IF 1.4) Pub Date : 2023-09-30 Carolina Cornejo,Tatsuya Otani,Nobuhiro Suzuki,Ludwig Beenken
Cryphonectria carpinicola is an ascomycetous fungus that has been regularly found in its asexual form on European hornbeam (Carpinus betulus) in Europe over the past two decades. Here we describe the discovery of C. carpinicola in Japan and report for the first time its sexual state on Carpinus species. No symptomatic trees were observed, but stromata were found saprotrophically on broken branches
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A new species of the genus Aleurodiscus sensu lato (Russulales, Basidiomycota) from Hachijo Island, Japan. Mycoscience (IF 1.4) Pub Date : 2023-07-31 Nitaro Maekawa,Ryo Sugawara,Ryo Nakano,Ryotaro Shino,Kozue Sotome,Akira Nakagiri,Yuichi Oba
Aleurodiscus sagittisporus sp. nov. is described and illustrated. This species is characterized by producing basidiomata with a monomitic hyphal system, clampless-septate hyphae, arrowhead-shaped, amyloid, finely verrucose basidiospores, gloeocystidia, dendrohyphidium-like branched paraphysoid hyphae, and variously shaped swelling cells in the hymenium. Phylogenetic analyses based on nuclear rDNA LSU
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Hydropodia silvae-nipponicae (Porotheleaceae), a new species from evergreen broad-leaved forests in Japan. Mycoscience (IF 1.4) Pub Date : 2023-07-31 Taiga Kasuya,Reiichiro Kaneko,Seiji Takehashi,Kentaro Hosaka
Hydropodia silvae-nipponicae, a new agaric species was described based on the specimens collected from evergreen broad-leaved forests in Japan. The present new species is distinct from H. subalpina, the type species of the genus by morphological characteristics and nuclear rDNA sequences. Hydropodia silvae-nipponicae is characterized by its nodulose cystidium-like terminal elements of pileipellis,
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Molecular phylogeny and morphology reveal a new wood-rotting fungal species, Sistotrema yunnanense sp. nov. from the Yunnan-Guizhou Plateau. Mycoscience (IF 1.4) Pub Date : 2023-05-15 Li-Qiong Cai,Chang-Lin Zhao
Wood-rotting fungi are important components of woody plant ecosystems and play an active role in the decomposition and turnover of nutrients from wood, and are among the major groups of Basidiomycota. In this study, a new species of wood-rotting fungus, Sistotrema yunnanense, was proposed based on morphological characteristics and molecular evidence. It is characterized by resupinate basidiomata, a
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Rhodocybe subasyae, a new species of Rhodocybe sect. Rufobrunnea (Entolomataceae, Agaricales) from northeast China Mycoscience (IF 1.4) Pub Date : 2023-05-02 Sun Ya-Li, Bau Tolgor
Rhodocybe subasyae is described from northeast China as a new species with both morphological and molecular characteristics, which is morphologically characterized by its tricholomatoid basidiomata, orange white to beige red pileus, adnexed to sinuate lamellae and long clavate branched cheilocystidia within section Rufobrunnea. The phylogenetic tree based on internal transcribed spacers of rDNA (nrITS)
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Re-examination of a rare protosteloid amoeba Schizoplasmodiopsis micropunctata, and the revision of Tychosporium (Cavosteliida, Variosea, Amoebozoa). Mycoscience (IF 1.4) Pub Date : 2023-03-20 Yoshiaki Iwamoto,Yousuke Degawa,Takeshi Nakayama
The genus Schizoplasmodiopsis is one of the most morphologically diverse groups among the class Variosea. Recent phylogenetic studies suggest that Schizoplasmodiopsis is polyphyletic, but there are few taxonomic studies of this genus. We established S. micropunctata strain YIP-40, observed in detail its of morphology and lifecycle, and conducted a phylogenetic analysis. The phylogenetic analysis revealed
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Isolation and identification of an arbuscular mycorrhizal fungus specifically associated with mycoheterotrophic seedlings of Gentiana zollingeri (Gentianaceae) Mycoscience (IF 1.4) Pub Date : 2023-02-28 Ryota Kusakabe, Masahide Yamato
Gentiana zollingeri (Gentianaceae) is an initial mycoheterotrophic plant that depends on a specific group of arbuscular mycorrhizal (AM) fungi for carbon source during underground growth after seed germination. In this study, a mycorrhizal fungus dominant in mycoheterotrophic seedlings of G. zollingeri was successfully isolated from a soil core collected from a point close to a flowering G. zollingeri
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Preliminary exploration on the ectomycorrhizal status of a wild edible Gomphus species from Southwest China. Mycoscience (IF 1.4) Pub Date : 2023-02-20 Yangyang Geng,Shixin Zhang,Ningxian Yang,Likang Qin
A wild edible Gomphus species was discovered at local wild mushroom markets from May to November in Southwest China, where it was eaten for hundreds of years. However, litter information on the taxonomy is available. Whether Gomphus is a saprotrophic, parasitic, or ectomycorrhizal (ECM) fungus is unclear. In the present study, field investigation, fungi isolation, optimum medium, morphological description
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Taxonomic re-examination of Japanese isolates of the genus Lasiodiplodia. Mycoscience (IF 1.4) Pub Date : 2023-02-20 Yukako Hattori,Lynn Nakano,Chiharu Nakashima
Lasiodiplodia species cause dieback, shoot blight, fruit rot, and stem-end rot of woody plants. Recently, studies on taxonomic revision of Lasiodiplodia species have been conducted globally and have led to more clarification of the species diversity of this genus. However, only three species of the genus have been reported in Japan. To evaluate the species diversity of Lasiodiplodia in Japan, we re-examined
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A new species of Neofavolus (Polyporales, Basidiomycota) from Brazil Mycoscience (IF 1.4) Pub Date : 2023-02-17 Alex Almeida Alcantara, Ricardo Matheus Pires, Adriana de Mello Gugliotta
Neofavolus teixeirae sp. nov. (Basidiomycota) is described and illustrated based on specimens collected from a reforestation area in southeastern Brazil. This new species is characterized by a lateral stipe up to 1.3 cm long, lacerate and angular pores measuring 0.5–2 (–2.5) per mm, and cylindrical to subcylindrical basidiospores. Phylogenetic analyses of the ITS and LSU regions confirmed its phylogenetic
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Hypochnicium sensu lato (Polyporales, Basidiomycota) from Japan, with descriptions of a new genus and three new species. Mycoscience (IF 1.4) Pub Date : 2023-01-31 Nitaro Maekawa,Ryo Sugawara,Hiroki Kogi,Shungo Norikura,Kozue Sotome,Naoki Endo,Akira Nakagiri,Shuji Ushijima
Species of Hypochnicium (Polyporales, Basidiomycota) collected from Japan were studied on their taxonomy by morphological and phylogenetic approaches. Phylogenetic analyses based on a nrDNA LSU and ITS dataset including the Japanese specimens and other publicly available ones show that Hypochnicium is polyphyletic. Since the clade containing the type species H. bombycinum was well-supported, we defined
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Polymerase chain reaction-based methods for the detection of heat-resistant ascomycetous fungi Mycoscience (IF 1.4) Pub Date : 2023-01-26 Takashi Yaguchi
There is increasing incidence of food spoilage and health hazards caused by heat-resistant fungi belonging to the genera Byssochlamys, Thermoascus, and Neosartorya, among others. Their ascospores cannot be sterilized by heating the food. The microbiological risk assessment studies of these fungi during the production of food and beverages indicated that these fungal species or genera in food are associated
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North-to-South diversity of lipomycetaceous yeasts in soils evaluated with a cultivation-based approach from 11 locations in Japan. Mycoscience (IF 1.4) Pub Date : 2022-12-26 Atsushi Yamazaki,Wanlapa Lorliam,Masataka Uchino,Ken-Ichiro Suzuki,Hiroko Kawasaki
To understand the species distribution, diversity, and density of lipomycetaceous yeasts in soil based on their north-to-south location in Japan, 1146 strains were isolated from soil samples at 11 locations from Hokkaido to Okinawa Prefecture and taxonomically characterized. Lipomycetaceous yeast strains were isolated efficiently from soil by selecting watery mucoid-like colonies on agar plates with
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DNA barcode sequencing discovered the aecial host of Puccinia chunjiei. Mycoscience (IF 1.4) Pub Date : 2022-12-26 Sarah Hambleton,Miao Liu
The telial stage of Puccinia chunjiei was described in 2012 from a single specimen collected in China (DAOM 240982). This species is the closest relative of P. graminis but differs in telial morphology on their grass hosts and in DNA sequences. As part of a DNA-barcoding project for rust herbarium specimens, collections of the aecial stage of P. graminis on Berberis were processed. For one specimen
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Characterization of Penicillium Species Isolated from Dioscorea polystachya in Hokkaido, Japan. Mycoscience (IF 1.4) Pub Date : 2022-12-26 Roselyn Jove Uy,Miyuki Kayamori,Chiharu Nakashima
Chinese yam (Dioscorea polystachya) has been cultivated as a food crop in Japan. The blue mold disease of Chinese yam during the storage and treatment of forced sprouting is one of the hindrances in attaining productivity that can lead to food security in tropical countries and steady farming that will eventually lead to farmers' profitability. In this study, three species of the genus Penicillium
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Mushroom poisoning in Thailand between 2003 and 2017. Mycoscience (IF 1.4) Pub Date : 2022-11-01 Sayanh Somrithipol,Umpawa Pinruan,Sujinda Sommai,Phongsawat Khamsuntorn,Janet Jennifer Luangsa-Ard
The frequent occurrence of mushroom poisoning cases in the wet season in Thailand has long been recognized but has never been quantitatively analyzed. This study aims to analyze mushroom poisoning cases in Thailand between 2003 and 2017 and focused on their association with the rainfall. The results revealed 22,571 cases and 106 deaths in this period. Cases were higher for females than males, adults
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A new edible species of Gomphus (Gomphaceae) from southwestern China. Mycoscience (IF 1.4) Pub Date : 2022-10-21 Jian-Wei Liu,Thatsanee Luangharn,Shan-Ping Wan,Ran Wang,Fu-Qiang Yu
Gomphus matijun, a new edible species, is described from southwestern China based on phylogenetic and morphological evidence. Phylogenetic analyses of the nrLSU and ITS datasets indicated that G. matijun is related to G. crassipes and G. ludovicianus with weak statistical support in maximum likelihood but strong statistical support in Bayesian analyses. Gomphus matijun is characterized by its gray-blue
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Novel acid trehalase belonging to glycoside hydrolase family 37 from Pleurotus sp.: cloning, expression and characterization. Mycoscience (IF 1.4) Pub Date : 2022-10-21 Gaku Tsutsumi,Chikako Kuroki,Kengo Kamei,Mizuho Kusuda,Masami Nakazawa,Tatsuji Sakamoto,Mariko Ishikawa,Shinji Harada,Hitoshi Kobayashi,Kenji Ouchi,Satoshi Inatomi,Minoru Sakaguchi,Takeo Iwamoto,Mitsuhiro Ueda
The N-terminal amino acid sequence of the Pleurotus sp. 90 kDa protein was in good agreement with the corresponding sequence of the glycoside hydrolase (GH) family 37 protein (trehalase) from P. ostreatus PC 15 v2.0. The length of the Pleurotus sp. trehalase gene was 2247 bp, encoding a protein of 749 amino acids with a predicted molecular mass of 81.2 kDa. The molecular mass of the recombinant enzyme
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Morchella nipponensis sp. nov. (Ascomycota, Pezizales): a paleoendemic species of section Morchella discovered in Japan. Mycoscience (IF 1.4) Pub Date : 2022-10-21 Philippe Clowez,Takumi Izumi,Paul-Bill Lamiable,Koichi Shibakusa,Camelia Minculeasa,Pablo Alvarado
A previously unknown morel species apparently endemic to Japan is here described. Morchella nipponensis is proposed for this species. This new taxon displays archaic features recalling section Rufobrunnea (pileus lanceolate, few primary alveoli), and a yellow pileus similar to subsection Sceptriformis of section Morchella. The phylogenetic analysis of ITS rDNA, as well as RPB1, RPB2 and TEF1 genes
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New findings on the fungal species Tricholoma matsutake from Ukraine, and revision of its taxonomy and biogeography based on multilocus phylogenetic analyses. Mycoscience (IF 1.4) Pub Date : 2022-09-30 Wataru Aoki,Niclas Bergius,Serhii Kozlan,Fuminori Fukuzawa,Hitomi Okuda,Hitoshi Murata,Takahide A Ishida,Lu-Min Vaario,Hisayasu Kobayashi,Erbil Kalmiş,Toshimitsu Fukiharu,Seiki Gisusi,Ken-Ichi Matsushima,Yoshie Terashima,Maki Narimatsu,Norihisa Matsushita,Kang-Hyeon Ka,Fuqiang Yu,Takashi Yamanaka,Masaki Fukuda,Akiyoshi Yamada
Matsutake mushrooms are among the best-known edible wild mushroom taxa worldwide. The representative Tricholoma matsutake is from East Asia and the northern and central regions of Europe. Here, we report the existence of T. matsutake under fir trees in Eastern Europe (i.e., Ukraine), as confirmed by phylogenetic analysis of nine loci on the nuclear and mitochondrial genomes. All specimens from Japan
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Puccinia caricis-smilacis, a new caricicolous rust fungus producing spermogonial and aecial stages on Smilax china in Japan. Mycoscience (IF 1.4) Pub Date : 2022-09-30 Taiga Kasuya,Kentaro Hosaka,Makoto Kakishima
Puccinia caricis-smilacis, a new species of caricicolous rust fungus was described based on morphological characteristics and nuclear rDNA sequences from Japan. The heteroecious life cycle of P. caricis-smilacis was elucidated via field observations, inoculation experiments and molecular analyses. This rust fungus produces uredinial and telial stages on Carex fibrillosa, and spermogonial and aecial
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Phylogeny and taxonomy of Erysiphe berberidis (s. lat.) revisited. Mycoscience (IF 1.4) Pub Date : 2022-09-30 Li Liu,Michael Bradshaw,Uwe Braun,Monika Götz,Seyed Akbar Khodaparast,Tie-Zhi Liu,Timur S Bulgakov,Hamideh Darsaraei,Wolfgang Karl Hofbauer,Yu Li,Shu-Yan Liu
Phylogenetic and morphological analyses have been conducted on powdery mildew specimens on different Berberis and Mahonia spp. from Asia, Europe and North America. The present study showed that collections of Erysiphe berberidis exhibit a high degree of morphological plasticity of the sexual morph, in contrast to their morphologically, rather uniform, asexual morph. In phylogenetic tree, all sequences
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Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies. Mycoscience (IF 1.4) Pub Date : 2022-09-20 Akiyoshi Yamada
Most edible ectomycorrhizal mushrooms are harvested in forests or controlled tree plantations; examples include truffles, chanterelles, porcinis, saffron milk caps, and matsutake. This study explored recent advances in in vitro ectomycorrhizal cultivation of chanterelles and matsutakes for successful ectomycorrhizal seedling establishment and the subsequent manipulation of these seedlings for efficient
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Lentinula ixodes comb. nov. (Omphalotaceae, Agaricales) including new records in Brazil. Mycoscience (IF 1.4) Pub Date : 2022-09-20 Jadson J S Oliveira,Tiara S Cabral,Ruby Vargas-Isla,José F B Silva,Doriane P Rodrigues,Nelson Menolli,Mariana P Drewinski,Noemia K Ishikawa
In the search for new strains of edible mushrooms in the Brazilian Amazon Forest, we found Lentinula specimens different from Lentinula raphanica. These were described morphologically and evaluated phylogenetically within the Lentinula clade. The mating system was determined, and interbreeding compatibility with L. raphanica was verified. The basidiomata have a cinnamon or deep orange to fulvous brown
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Taxonomy and phylogeny of Exobasidium pentasporium causing witches' broom of Rhododendron species. Mycoscience (IF 1.4) Pub Date : 2022-09-20 Saho Shibata,Yuuri Hirooka
Exobasidium pentasporium was first found on Rhododendron kaempferi in Nikko, Tochigi Prefecture, Japan and described only with a brief mentions and illustration of a specimen in 1896. This fungus causes a witches' broom disease of Rhododendron species. To stabilize the concept of this species, the specimen in the protologue was located, carefully examined, and illustrated. In addition, the name was
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Micropsalliota pileocystidiata (Agaricaceae), a new species from Maharashtra, India. Mycoscience (IF 1.4) Pub Date : 2022-09-08 Prashant B Patil,Sharda Vaidya,Satish Maurya,Nitinkumar P Patil
A new species of Micropsalliota is described from tropical region of Maharashtra, India. The species is recognized on the basis of morphological details and its phylogenetic placement is determined by using of nrITS and nrLSU sequence data analyses. Micropsalliota pileocystidiata is characterised by its robust basidiomes covered by reddish brown appressed fibrillose squamules, ellipsoid to amygdaliform
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Pyrenopeziza orientalipetiolaris sp. nov. in Japan and morphological and genetic comparison with its relevant species P. petiolaris in Europe. Mycoscience (IF 1.4) Pub Date : 2022-08-11 Hiyori Itagaki,Tsuyoshi Hosoya
Some Asian fungi are morphologically very similar to European species but belong to different species. A fungus that resembles Pyrenopeziza petiolaris, which commonly occurs on the petioles of Acer pseudoplatanus in Europe, was found on the petioles of Acer spp. and other tree leaves in Japan. The apothecia of this fungus were smaller than those of P. petiolaris, suggesting that it is a different species
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The study of early screening technique for fruiting ability of Lentinula edodes hybrid progenies. Mycoscience (IF 1.4) Pub Date : 2022-08-11 Yu Li,Hailong Yu,Liangmin Li,Chunyan Song,Xiaodong Shang,Qi Tan,Lujun Zhang,Qiaozhen Li
Crossbreeding is the most commonly used method in breeding of Lentinula edodes, however low fruiting rate of the hybrids has always caused troubles and barriers for breeders. An early screening method of the fruiting ability could make the breeding work more efficient. In this paper, a rapid and high-throughput laccase activity detection method based on agar diffusion principle was developed. In this
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Soil propagule banks of ectomycorrhizal fungi associated with Larix cajanderi above the treeline in the Siberian Arctic. Mycoscience (IF 1.4) Pub Date : 2022-07-31 Yumiko Miyamoto,Trofim C Maximov,Alexander Kononov,Atsuko Sugimoto
Microbial symbionts are essential for plant niche expansion into novel habitats. Dormant propagules of ectomycorrhizal (EM) fungi are thought to play an important role in seedling establishment in invasion fronts; however, propagule bank communities above the treeline are poorly understood in the Eurasian Arctic, where treelines are expected to advance under rapid climate change. To investigate the
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Revisiting the isolation source after the first discovery: Myconymphaea yatsukahoi on excrements of Lithobiomorpha (Chilopoda). Mycoscience (IF 1.4) Pub Date : 2022-07-31 Yusuke Takashima,Mai Suyama,Kohei Yamamoto,Tomohiko Ri,Kazuhiko Narisawa,Yousuke Degawa
Myconymphaea yatsukahoi is a fungus that has only been isolated once from a forest in the Sugadaira Research Station, Nagano, Japan. Over 20 y have passed since its first discovery but since then it has not been rediscovered. Here, we re-isolated M. yatsukahoi from the type locality and another location, Tambara Moor, Gunma, Japan. Sporophores of this species were detected by direct field observation
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The asexual morph and molecular phylogeny of endemic Phyllactinia verruculosa on Indigofera tinctoria. Mycoscience (IF 1.4) Pub Date : 2022-07-31 Shu-Rong Tang,Shu-Yan Liu,Jing Feng
Phyllactinia verruculosa is a powdery mildew species (Erysiphaceae, tribe Phyllactinieae) so far only known from its type material collected in China on Indigofera scabrida in 1992, which only comprised the sexual morph. Two asexual morph samples were observed, one was collected on I. tinctoria on the campus of Guizhou University, Guiyang, China, and another one, on I. scabrida, was borrowed from Kunming
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Erysiphe ruyongzhengiana sp. nov., a new powdery mildew species on Aristolochia debilis, belonging to the Erysiphe aquilegiae clade. Mycoscience (IF 1.4) Pub Date : 2022-07-31 Li Liu,Lin-Chong Hui,Shou-Rong Yu,Yu Li,Shu-Yan Liu
Powdery mildew was found on Aristolochia debilis (Aristolochiaceae) in Jiangsu Province and Shandong Province, China. This fungus is characterized by having long conidiophore foot-cells which are straight or curved at the base, and chasmothecia with numerous appendages. Phylogenetic analysis using internal transcribed spacer sequences showed that five sequences on A. debilis determined in this study
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Characterization of fungal microbial diversity in Tibetan sheep, Tibetan gazelle and Tibetan antelope in the Qiangtang region of Tibet. Mycoscience (IF 1.4) Pub Date : 2022-07-31 Zhenda Shang,Zhankun Tan,Qinghui Kong,Peng Shang,Honghui Wang,Wangjie Zhaxi,Ciren Zhaxi,Suozhu Liu
Due to the high crude fiber content, straw of various crops is difficult to become a high quality forage resource. The degradation of cellulose in nature mainly depends on the cellulase secreted by microbes, which degrade cellulose into small molecular substances through chemical action, and the microbes that secrete cellulase mainly include some bacteria, fungi and actinomycetes, etc. The large and
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Lamproderma vietnamense: a new species of myxomycetes with reticulate spores from Phia Oắc - Phia Đén National Park (northern Vietnam) supported by molecular phylogeny and morphological analysis. Mycoscience (IF 1.4) Pub Date : 2022-07-31 Yuri K Novozhilov,Ilya S Prikhodko,Nadezhda A Fedorova,Oleg N Shchepin,Vladimir I Gmoshinskiy,Martin Schnittler
A new species of Lamproderma (Myxomycetes), described herein as L. vietnamense, was recovered in the field on ground litter from mountain subtropical forests (Phia Oắc - Phia Đén National Park) of northern Vietnam. Morphological details were examined by light and scanning electron microscopy. The species is characterized by a distinct and unique combination of morphological features, including a bright
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In-vitro symbiotic germination of seeds of five mycoheterotrophic Gastrodia orchids with Mycena and Marasmiaceae fungi. Mycoscience (IF 1.4) Pub Date : 2022-05-31 Michiru Kitahara,Kosuke Nagamune,Akihiko Kinoshita,Chigusa Yugeta,Naoto Ohara,Akane Shimazaki,Yumi Yamashita,Tomohisa Yukawa,Naoki Endo,Yuki Ogura-Tsujita
We performed in-vitro germination tests on seeds from five Gastrodia orchids (G. confusa, G. elata var. elata, G. elata var. pallens, G. nipponica, and G. pubilabiata) using one Marasmiaceae and two Mycena isolates. Mycena sp. 1 promoted germination of all five Gastrodia orchids, with root and/or tuber formation observed in G. confusa, G. nipponica, and G. pubilabiata. No additional growth was observed
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Taxonomic revision of the Typhula ishikariensis complex. Mycoscience (IF 1.4) Pub Date : 2022-05-31 Tamotsu Hoshino,Oleg B Tkachenko,Motoaki Tojo,Anne Marte Tronsmo,Taiga Kasuya,Naoyuki Matsumoto
Typhula ishikariensis and the related fungi were separated into three biological species by morphological and physiological characteristics, as well as DNA sequences and mating reactions. We propose that the T. ishikariensis complex should be divided into three species (T. ishikariensis, T. canadensis and T. hyperborea) and two varieties (T. ishikariensis var. ishikariensis and var. idahoensis). Typhula
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Japanese "nameko" mushrooms (Pholiota microspora) produced via sawdust-based cultivation exhibit severe genetic bottleneck associated with a single founder. Mycoscience (IF 1.4) Pub Date : 2022-05-31 Akira S Hirao,Atsushi Kumata,Toshihito Takagi,Yoshito Sasaki,Takashi Shigihara,Eiichi Kimura,Shingo Kaneko
Pholiota microspora ("nameko" in Japanese) is one of the most common edible mushrooms, especially in Japan, where sawdust-based cultivation is the most dominant method accounting for 99% of the production. The current strains for sawdust cultivation in Japan are considered to have been derived from a single wild strain collected from Fukushima, Japan, implying that commercial nameko mushrooms are derived
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Effect of sodium chloride on basidiospore germination and vegetative mycelial growth of the ectomycorrhizal fungus Rhizopogon roseolus. Mycoscience (IF 1.4) Pub Date : 2022-05-31 Shota Nakano,Qi Gao,Tadanori Aimi,Norihiro Shimomura
Rhizopogon roseolus is a basidiomycetous ectomycorrhizal fungus that inhabits mainly coastal areas. Understanding the response of this fungus to salinity at each stage of its life cycle will lead to elucidation of the strategies for its propagation. This study examined the effect of sodium chloride (NaCl) on basidiospore germination and mycelial growth of both homokaryotic and heterokaryotic strains
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Two new species of Sistotrema s.l. (Cantharellales) from Japan with descriptions of their ectomycorrhizae. Mycoscience (IF 1.4) Pub Date : 2022-05-31 Ryo Sugawara,Nana Shirasuka,Tatsuki Yamamoto,Kosuke Nagamune,Kaito Oguchi,Nitaro Maekawa,Kozue Sotome,Akira Nakagiri,Shuji Ushijima,Naoki Endo
We describe two new species of resupinate Sistotrema sensu lato (Cantharellales) collected in Japan: S. flavorhizomorphae and S. chloroporum. Both species have urniform basidia with more than four sterigmata and monomitic hyphal system, oil-rich hyphae in subiculum, which is typical for this genus. Sistotrema chloroporum is characterized by poroid hymenophore partly yellowish-green, basidia 4-6-spored
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Euantennaria pleioblasti sp. nov. (Euantennariaceae) and Metacapnodium cf. quinqueseptatum (Metacapnodiaceae), two mixed sooty moulds in subicula on Pleioblastus sp. in Taiwan. Mycoscience (IF 1.4) Pub Date : 2022-03-20 Junta Sugiyama,Tsuyoshi Hosoya
Black subicula, comprising a mixture of two sooty moulds of Euantennariaceae and Metacapnodiaceae, on Pleioblastus were collected in Batongguan, alt. ca. 2800 m, Nantou County, Taiwan in 1984. The former sooty mould is described and illustrated as Euantennaria pleioblasti sp. nov., an asexually typified species of the genus, as currently circumscribed with the application of the single name nomenclature
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Two new species of Steccherinum (Polyporales, Basidiomycota) from southern China based on morphology and DNA sequence data. Mycoscience (IF 1.4) Pub Date : 2022-03-20 Jun-Hong Dong,Ya-Xing Wu,Chang-Lin Zhao
Two new wood-inhabiting fungal species, Steccherinum hirsutum and S. yunnanense spp. nov., are proposed based on a combination of morphological features and molecular evidence. Sequences of internal transcribed spacer and large subunit region of nuculear ribosomal RNA gene of the studied samples were generated, and phylogenetic analyses were performed using maximum likelihood, maximum parsimony and
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Physiological characteristics of pure cultures of a white-colored truffle Tuber japonicum. Mycoscience (IF 1.4) Pub Date : 2022-03-20 Shota Nakano,Akihiko Kinoshita,Keisuke Obase,Noritaka Nakamura,Hitomi Furusawa,Kyotaro Noguchi,Takashi Yamanaka
A white-colored truffle Tuber japonicum, indigenous to Japan, is an ascomycetous ectomycorrhizal fungus. To clarify the physiological characteristics of this fungus, we investigated the influence of culture medium, temperature, and sources of nitrogen (N) and carbon (C) on the growth of five strains. Tuber japonicum strains grew better on malt extract and modified Melin-Norkrans medium, and showed
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Morpho-anatomical and molecular characterization of a native mycorrhizal Amanita species associated with Guapira opposita (Nyctaginaceae) in the brazilian Atlantic Forest. Mycoscience (IF 1.4) Pub Date : 2022-03-20 Ariadne Nóbrega Marinho Furtado,Ornella Comandini,Marco Leonardi,Andrea C Rinaldi,Maria Alice Neves
In this work, we characterize naturally occurring mycorrhizae formed by Amanita viscidolutea on Guapira opposita in the Atlantic Forest in Brazil. We sequenced the rDNA ITS region from the mycorrhizae and basidiomata to identify both symbionts. Amanita viscidolutea mycorrhizae were up to 43 mm long, mostly simple, and unbranched to irregularly pinnate. The fungal mantle surface was velvety to slightly
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Association between corticolous myxomycetes and tree vitality in Cryptomeria japonica. Mycoscience (IF 1.4) Pub Date : 2022-03-20 Kazunari Takahashi,Yu Fukasawa
The bark of live trees provides an important microhabitat for corticolous myxomycetes. However, the association between the presence of myxomycetes and health of host trees has not been studied in detail. In this study, we aimed to investigate the relationship between tree vitality and myxomycetes on the bark of Cryptomeria japonica trees in a montane forest in western Japan. The vitality of trees
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Tuber torulosum: A new truffle species decorated with moniliform cystidia from Japan. Mycoscience (IF 1.4) Pub Date : 2022-01-20 Akihiko Kinoshita,Kohei Yamamoto,Toshiyuki Tainaka,Toshifumi Handa,Akiyoshi Yamada
We describe a new truffle species, Tuber torulosum, based on molecular and morphological analyses. This species forms a single globose ascospore per ascus, pale yellow in color, as do Japanese T. flavidosporum and Chinese T. turmericum and T. xanthomonosporum in the Japonicum clade of the Tuber phylogeny. However, it can be distinguished from them microscopically by its whitish tomentose mycelium that
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Taxonomic revision of Termitomyces species found in Ryukyu Archipelago, Japan, based on phylogenetic analyses with three loci. Mycoscience (IF 1.4) Pub Date : 2022-01-20 Yuuki Kobayashi,Miyuki Katsuren,Masaru Hojo,Shohei Wada,Yoshie Terashima,Masayoshi Kawaguchi,Gaku Tokuda,Kazuhiko Kinjo,Shuji Shigenobu
Fungi in the genus Termitomyces are external symbionts of fungus-growing termites. The three rhizogenic Termitomyces species T. eurrhizus, T. clypeatus, and T. intermedius, and one species similar to T. microcarpus that lacks pseudorrhiza, have been reported from Ryukyu Archipelago, Japan. In contrast, only two genetic groups (types A and B) of Termitomyces vegetative mycelia have been detected in
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Morphological characteristics of ectomycorrhizas formed by in vitro synthesis between conifer seedlings and Tuber mycelial strains of the Puberulum clade isolated in Japan. Mycoscience (IF 1.4) Pub Date : 2022-01-20 Keisuke Obase
Seedlings of Pinus densiflora and Abies sachalinensis were inoculated with Tuber mycelial strains of the Puberulum clade in vitro to examine the morphological characteristics of their ectomycorrhizas. Axenically germinated seedlings were inoculated with the mycelia of five taxa from the Puberulum clade and grown in glass jars for 4 mo in an illuminated incubator. The seedlings were successfully colonized
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Description of a new species of Gerhardtia (Lyophyllaceae, Agaricales) from Japan based on morphological and molecular phylogenetic analyses and live culture characteristics. Mycoscience (IF 1.4) Pub Date : 2021-12-18 Naoki Endo,Moe Takahashi,Kosuke Nagamune,Kaito Oguchi,Ryo Sugawara,Kozue Sotome,Akira Nakagiri,Nitaro Maekawa
We describe a new species of Gerhardtia from Japan based on basidiomata morphology, live culture characteristics, and molecular phylogenetic analyses. Gerhardtia venosolamellata is found on broad-leaf litter, and is characterized by tricholomatoid to marasmioid basidiomata, an off-white to pale salmon-pink pileus surface with faint marginal striae, subdistant lamellae with lateral veins, a tomentose
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Strong primer bias for Tulasnellaceae fungi in metabarcoding: Specific primers improve the characterization of the mycorrhizal communities of epiphytic orchids. Mycoscience (IF 1.4) Pub Date : 2021-11-20 Kento Rammitsu,Tadashi Kajita,Ryosuke Imai,Yuki Ogura-Tsujita
Primer bias toward Tulasnellaceae fungi during PCR is a known issue with metabarcoding analyses for the assessment of orchid mycorrhizal communities. However, this bias had not been evaluated for the fungal communities of epiphytic orchids, which account for 69% of all orchid species diversity. We compared the mycorrhizal communities detected using two primer pairs, a fungal universal primer pair (ITS86F/ITS4)
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Phylogenetic relationships among fern rust fungi and Desmella lygodii comb. nov. Mycoscience (IF 1.4) Pub Date : 2021-11-20 Izumi Okane,Yoshitaka Ono,Katsura Ohmachi,M Catherine Aime,Yuichi Yamaoka
The rust fungi (Pucciniales) that infect ferns, early diverging vascular plants, are neither "primitive" nor monophyletic, as once hypothesized. The neotropical fern pathogen, Puccinia lygodii (Pucciniaceae), specializes on species of Lygodium. Lygodium is believed to have evolved in a period ca. 211 mya, which is after the evolution of the temperate fern rust fungi that parasitize later diverged ferns
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Taxonomy of corticioid fungi in Japan : Present status and future prospects. Mycoscience (IF 1.4) Pub Date : 2021-11-20 Nitaro Maekawa
Corticioid fungi form effused basidiomata with various hymenophore configurations, mostly on woody substrates, and they are presently classified into 15 orders in Agaricomycotina in the phylum Basidiomycota. In this review, the corticioid fungi of Japan are treated on the basis of the current classification system, and all currently known species are listed and classified by order. Japanese corticioid
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Isolation, identification, and characterization of wild budding yeasts from rose flowers in Fukuyama city, Hiroshima, Japan, and their application in bread and wine production. Mycoscience (IF 1.4) Pub Date : 2021-11-20 Taisuke Hisatomi,Kousuke Toyomura
In this study, we isolated 741 wild budding yeast strains from the flowers of 45 rose cultivars growing in Fukuyama city, Hiroshima, Japan. Of these 741 strains, 21 were found to have high fermentation abilities in yeast extract-peptone-dextrose (YPD) medium. Four of the 21 strains were able to ferment bread dough to make bread. These yeasts were identified as Saccharomyces cerevisiae, Lachancea fermentati