-
High density linkage to physical mapping in a unique Tall x Dwarf Coconut (Cocos nucifera L.) outbred F2 uncovers a major QTL for flowering time colocalized with the FLOWERING LOCUS T (FT) bioRxiv. Plant Biol. Pub Date : 2024-03-27 Dario Grattapaglia, Wellington Bruno dos Santos Alves, Cleso Antonio Patto Pacheco
Introduction: The coconut tree crop (Cocos nucifera L.) provides vital resources for millions of people worldwide. Coconut germplasm is largely classified into Tall (Typica) and Dwarf (Nana) types. While Tall coconuts are outcrossing, more productive, stress tolerant and late flowering, Dwarf coconut are inbred, flower early with a high rate of bunch emission. Precocity determines earlier production
-
ER-anchored protein sorting controls the fate of two proteasome activators for intracellular organelle communication during proteotoxic stress bioRxiv. Plant Biol. Pub Date : 2024-03-27 Gautier Langin, Margot Raffeiner, David Biermann, Mirita Franz-Wachtel, Daniela Spinti, Frederik Börnke, Boris Macek, Suayib Üstün
Proteotoxic stress, characterized by the accumulation of damaged proteins, poses a significant challenge to cellular homeostasis. To mitigate proteotoxicity eukaryotes employ the proteasome that is regulated by proteasome activators, e.g. transcription factors that promote gene expression of proteasome subunits. As proteotoxicity originates in different compartments, cells need to perceive signals
-
MOS4-Associated Complex subunits 3A and 3B modulate FLM splicing to repress photoperiod-dependent floral transition bioRxiv. Plant Biol. Pub Date : 2024-03-27 Yu-Wen Huang, Chih-Yen Tseng, Yi-Tsung Tu, Hsin-Yu Hsieh, Yu-Sen Wang, Yu-Zhen Chen, Yun-Tung Ly, Shih-Long Tu, Chin-Mei Lee
Plants adjust their flowering time by integrating environmental cues through complex regulatory networks. RNA splicing plays a crucial role in modulating gene expression in response to flowering signals. The MOS4-associated complex (MAC), consisting of the evolutionarily conserved E3 ubiquitin ligases MAC3A and MAC3B, is pivotal in splicing regulation. However, their involvement in floral transition
-
Demographic and genetic impacts of powdery mildew in a young oak cohort bioRxiv. Plant Biol. Pub Date : 2024-03-27 Benoit Barrès, Cyril Dutech, Gilles Saint-Jean, Catherine Bodénès, Christian Burban, Virgil Fievet, Camille Lepoittevin, Pauline Garnier-Géré, Marie-Laure Desprez-Loustau
The demographic and genetic impacts of powdery mildew on the early stages of an oak population were studied in an ad hoc field design with two disease exposures. This enabled a detailed phenotypic monitoring of 1,733 emerging individuals from 15 progenies over nine years, and the genotyping of 68% of them. The pathogen induced high levels of seedling mortality several years after sowing, associated
-
Differential prolyl hydroxylation by six Physcomitrella prolyl-4 hydroxylases bioRxiv. Plant Biol. Pub Date : 2024-03-27 Christine Rempfer, Sebastian N.W. Hoernstein, Nico van Gessel, Andreas W. Graf, Roxane P. Spiegelhalder, Anne Bertolini, Lennard L Bohlender, Juliana Parsons, Eva L. Decker, Ralf Reski
The hydroxylation of proline residues to 4-trans-hydroxyproline (Hyp) is a common post-translational protein modification in plants, mediated by prolyl 4-hydroxylases (P4Hs). Hyps predominantly occur in a group of cell wall proteins, the Hydroxyproline-rich glycoproteins (HRGPs), where they are frequently O-glycosylated. While prolyl-hydroxylation and O-glycosylation are important, e.g. for cell wall
-
Chara canescens part I: Oospore differentiation of parthenogenic and dioecious strains and salt-dependent oospore sizes bioRxiv. Plant Biol. Pub Date : 2024-03-27 Anja Holzhausen, Roman Romanov, Hendrik Schubert
Chara canescens Loisel. is one of two European species of the section Desvauxia R.D. Wood of the genus Chara L. Whereas most populations of C. canescens reproduce parthenogenetically, a few sites with sexual reproducing populations are known. Studies of European C. canescens oospore morphology led to open questions about the taxonomic status. Here we investigated nearly 1000 oospores from 16 European
-
Dramatic Changes in Mitochondrial Subcellular Location and Morphology Accompany Activation of the CO2 Concentrating Mechanism bioRxiv. Plant Biol. Pub Date : 2024-03-27 Justin Findinier, Lydia-Marie Joubert, Michael F Schmid, Andrey Malkovskiy, Wah Chiu, Adrien Burlacot, Arthur R Grossman
Dynamic changes in intracellular ultrastructure can be critical for the ability of organisms to acclimate to environmental conditions. Microalgae, which are responsible for ~50% of global photosynthesis, compartmentalize their Rubisco into a specialized structure known as the pyrenoid when the cells experience limiting CO2 conditions; this compartmentalization appears to be a component of the CO2 Concentrating
-
Hyperspectral imaging for chloroplast movement detection bioRxiv. Plant Biol. Pub Date : 2024-03-27 Pawel Hermanowicz, Justyna Maria Labuz
We employed hyperspectral imaging to assess chloroplast positions in Nicotiana benthamiana and Arabidopsis thaliana leaves. In low light, chloroplasts move to cell walls perpendicular to the direction of the incident light. In high light, they move to cell walls parallel to the light direction. Chloroplast movements result in significant changes in leaf transmittance and reflectance. The changes in
-
Mutation of negative regulatory gene CEHC1 encoding an FBXO3 protein results in normoxic expression of HYDA genes in Chlamydomonas reinhardtii bioRxiv. Plant Biol. Pub Date : 2024-03-27 Xiaoqing Sun, Matthew LaVoie, Pete Lefebvre, Sean D Gallaher, Anne G Glaesener, Daniela Strenkert, Radhika Mehta, Sabeeha S Merchant, Carolyn D Silflow
Oxygen is known to prevent hydrogen production in Chlamydomonas, both by inhibiting the hydrogenase enzyme and by preventing transcription of HYDA-encoding genes. We screened for mutants showing constitutive accumulation of HYDA1 transcripts in the presence of oxygen. A reporter gene required for ciliary motility, placed under the control of the HYDA1 promoter, conferred motility only in hypoxic conditions
-
TFIIS is required for reproductive development and thermal adaptation in barley bioRxiv. Plant Biol. Pub Date : 2024-03-27 Imtiaz Ahmad, Kis Andras, Radhika Verma, Istvan Szadeczky-Kardoss, Henrik Mihaly Szaker, Aladar Pettko-Szandtner, Daniel Silhavy, Zoltan Havelda, Tibor Csorba
Regulation of transcriptional machinery and its adaptive role under different stress conditions are studied extensively in the dicot model plant Arabidopsis, but our knowledge on monocot species remains elusive. TFIIS is an RNA polymerase II associated transcription elongation co-factor. Previously it was shown that TFIIS ensures efficient transcription elongation that is necessary for heat stress
-
Glucosylceramides impact cellulose deposition and cellulose synthase complex motility in Arabidopsis bioRxiv. Plant Biol. Pub Date : 2024-03-27 Jose A. Villalobos, Rebecca E. Cahoon, Edgar B. Cahoon, Ian S. Wallace
Cellulose is an abundant component of plant cell wall matrices, and this para-crystalline polysaccharide is synthesized at the plasma membrane by motile Cellulose Synthase Complexes (CSCs). However, the factors that control CSC activity and motility are not fully resolved. In a targeted chemical screen, we identified the alkylated nojirimycin analog N-Dodecyl Deoxynojirimycin (ND-DNJ) as a small molecular
-
Diurnal regulation of SOS Pathway and Sodium Excretion Underlying Salinity Tolerance of Vigna marina bioRxiv. Plant Biol. Pub Date : 2024-03-27 Yusaku Noda, Fanmiao Wang, Sompong Chankaew, Hirotaka Ariga, Chiaki Muto, Yurie Iki, Haruko Ohashi, Yu Takahashi, Hiroaki Sakai, Koharo Iseki, Eri Ogiso-Tanaka, Nobuo Suzui, Young-Gen Yin, Yuta Miyoshi, Kazuyuki Enomoto, Naoki Kawachi, Prakit Somta, Jun Furukawa, Norihiko Tomooka, Ken Naito
Vigna marina (Barm.) Merr. is adapted to tropical marine beaches and has an outstanding tolerance to salt stress. Given there are growing demands for cultivating crops in saline soil or with saline water, it is important to understand how halophytic species are adapted to the saline environments. Here we sequenced the whole genome of V. marina with longreads, and performed a forward genetic study to
-
Telomere Length in Plants Estimated with Long Read Sequencing bioRxiv. Plant Biol. Pub Date : 2024-03-27 Kelly Colt, Semar Petrus, Bradley W Abramson, Allen Mamerto, Nolan T Hartwick, Todd P Michael
Telomeres play an important role in chromosome stability and their length is thought to be related to an organism's lifestyle and lifespan. Telomere length is variable across plant species and between cultivars of the same species, possibly conferring adaptive advantage. However, it is not known whether telomere length is related to lifestyle or life span across a diverse array of plant species due
-
Genome-Wide Identification and Comprehensive Analysis of the SBT Gene Family in Soybean (Glycine max) bioRxiv. Plant Biol. Pub Date : 2024-03-27 Xuan He, Shan hong He, Jia xin Yang, Zhi yong Yue
Background: The SBT (Subtilisin-like serine protease) protein, acting as a crucial serine protease, exerts multifaceted roles in orchestrating plant growth, development, and defense responses by modulating cell wall characteristics and activities of extracellular signaling molecules. Despite extensive investigations into SBT family genes in model dicotyledonous plants like Arabidopsis, no studies have
-
Genome-wide analysis of mRNA decay in Arabidopsis shoot and root reveals the importance of co-translational mRNA decay in the general mRNA turnover bioRxiv. Plant Biol. Pub Date : 2024-03-27 Marie-Christine Carpentier, Anne-Elodie Receveur, Alexandre Boubegtitene, Adrien Cadoudal, Cecile Bousquet-Antonelli, Remy Merret
Until recently, the general 5′-3′ mRNA decay was placed in the cytosol after the mRNA was released from ribosomes. However, the discovery of an additional 5′ to 3′ pathway, the Co-Translational mRNA Decay (CTRD), changed this paradigm. Up to date, defining the real contribution of CTRD in the general mRNA turnover has been hardly possible as the enzyme involved in this pathway is also involved in cytosolic
-
Autophagy at Crossroads: Modulating Responses to Combined Stresses, Unveiling Metabolic Shifts and Bacterial Dependencies bioRxiv. Plant Biol. Pub Date : 2024-03-27 Heike Seybold, Ella Katz, Yoram Soroka, Tamar Avin-Wittenberg
Plants face diverse stresses in natural environments, necessitating complex responses for survival. Abiotic and biotic stress responses are typically counteractive, posing challenges for breeding crops resilient to multiple stresses. Autophagy, a cellular transport process, plays a vital role in plant stress response, facilitating the degradation of cellular components and enabling nutrient recycling
-
Development of a tightly regulated copper-inducible transient gene expression system in Nicotiana benthamiana incorporating suicide exon and Cre recombinase bioRxiv. Plant Biol. Pub Date : 2024-03-27 Bing-Jen Chiang, Kuan-Yu Lin, Yi-Feng Chen, Ching-Yi Huang, Foong-Jing Goh, Lo-Ting Huang, Li-Hung Chen, Chih-Hang Wu
Chemical-inducible gene expression systems have been frequently used to regulate gene expression for functional genomics in various plant species. However, a convenient chemical-inducible system that can tightly regulate transgene expression in Nicotiana benthamiana is still missing. In this study, we developed a tightly regulated copper-inducible system that can be used to regulate transgene expression
-
Effect of temperature on circadian clock functioning of trees in the context of global warming bioRxiv. Plant Biol. Pub Date : 2024-03-27 Maximiliano Estravis-Barcala, Sofia Gaischuk, Marina Gonzalez-Polo, Alejandro Martinez-Meier, Rodrigo A Gutierrez, Marcelo J Yanovsky, Nicolas Bellora, Maria Veronica Arana
Plant survival in a warmer world requires the timely adjustment of biological processes to cyclical changes in the new environment. Circadian oscillators have been proposed to contribute to thermal adaptation and plasticity in plants, due to their ability to maintain periodicity in biological rhythms over a wide temperature range, promoting fitness. However, the influence of temperature and circadian
-
Ribosome biogenesis factor AtRRB1 confers pollen heat stress tolerance in Arabidopsis bioRxiv. Plant Biol. Pub Date : 2024-03-27 Chunlian Jin, Cedric Schindfessel, Limin Sun, Burcu Nur Keceli, Steffen Vanneste, Danny Geelen
The plant male reproductive system is very sensitive to high temperature stress leading to a reduction in fertility. Damage caused by heat stress is restored by the activation of transcription and the synthesis of chaperones that regulate the heat stress response. Here we report that AtRRB1 is a homolog of the yeast ribosome chaperone protein Rrb1p. AtRRB1 is an essential gene and a T-DNA insertion
-
Parameter variability makes a major contribution to uncertainty of crop phenology simulations bioRxiv. Plant Biol. Pub Date : 2024-03-27 Daniel Wallach, Taru Palosuo, Henrike Mielenz, Samuel Buis, Peter Thorburn, Senthold Asseng, Benjamin Dumont, Roberto Ferrise, Sebastian Gayler, Afshin Ghahramani, Matthew Tom Harrison, Zvi Hochman, Gerrit Hoogenboom, Mingxia Huang, Qi Jing, Eric Justes, Kurt Christian Kersebaum, Marie Launay, Elisabet Lewan, Ke Liu, Qunying Luo, Fasil Mequanint, Claas Nendel, Gloria Padovan, Jorgen Eivind Olesen,
Crop phenology has a major influence on crop yield and is a major aspect of crop response to global warming. Process-based models of phenology are often used to predict the effect of weather on the development rate of crops through their growth phases, but such models are associated with large uncertainties, as demonstrated by the large variability between simulation results of different models. The
-
A dual function of the IDA peptide in regulating cell separation and modulating plant immunity at the molecular level bioRxiv. Plant Biol. Pub Date : 2024-03-26 Vilde Olsson Lalun, Maike Breiden, Sergio Galindo-Trigo, Elwira Smakowska-Luzan, Rudiger Simon, Melinka Alonso Butenko
The abscission of floral organs and emergence of lateral roots in Arabidopsis is regulated by the peptide ligand INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) and the receptor protein kinases HAESA (HAE) and HAESA-LIKE 2 (HSL2). During these cell separation processes, the plant induces defense-associated genes to protect against pathogen invasion. However, the molecular coordination between abscission
-
Arabidopsis transcriptome responses to low water potential using high throughput plate assays bioRxiv. Plant Biol. Pub Date : 2024-03-25 Stephen Gonzalez, Joseph Swift, Adi Yaaran, Jiaying Xu, Charlotte Miller, Natanella Illouz-Eliaz, Joseph R Nery, Yotam Zait, Wolfgang Busch, Joseph R Ecker
Soil-free assays that induce water stress are routinely used to investigate drought responses in the plant Arabidopsis thaliana. Due to their ease of use, the research community often relies on polyethylene glycol (PEG), mannitol and salt (NaCl) treatments to reduce the water potential of agar media, and thus induce drought conditions in the laboratory. However, while these types of stress can create
-
An atlas of Brachypodium distachyon lateral root development bioRxiv. Plant Biol. Pub Date : 2024-03-25 Cristovao de Jesus Vieira Teixeira, Kevin Bellande, Alja van der Schuren, Devin O'Connor, Christian S. Hardtke, Joop Vermeer
The root system of plants is a vital part for successful development and adaptation to different soil types and environments. Besides allowing exploration of the soil for water and nutrients, it also provides anchorage. A major determinant of the shape of a plant root system is the formation of lateral roots, allowing for expansion of the root system. Arabidopsis thaliana, with its simple root anatomy
-
A comparative study of plant phenotyping workflows based on three-dimensional reconstruction from multi-view images bioRxiv. Plant Biol. Pub Date : 2024-03-25 Daiki Someno, Koji Noshita
With the world facing escalating food demand, limited agricultural land, and rapid environmental change, there is a growing need for data-driven sustainable agricultural management approaches. The proliferation of next-generation sequencers and sensor networks has reduced the cost of acquiring genomic and environmental data, respectively. However, collecting phenotypic data, which is crucial for monitoring
-
Unraveling the Role of 12-cis-Oxo-Phytodienoic Acid in the Wound Response of Arabidopsis thaliana: Insights from Transcriptomic and Complementation Studies bioRxiv. Plant Biol. Pub Date : 2024-03-25 Khansa Mekkaoui, Ranjit Baral, Fiona Smith, Moritz Klein, Ivo Feussner, Bettina Hause
In addition to jasmonoyl-isoleucine (JA-Ile), a well-established signaling molecule for plant growth and defense, its precursor, cis-12-oxo-phytodienoic acid (OPDA), is thought to possess independent signaling functions. Its perception in vascular plants is still uncharacterized. Several OPDA functions in Arabidopsis were inferred from a mutant that is affected in the function of the OPDA REDUCTASE3
-
Unveiling lipid chemodiversity in root exudates: A comprehensive characterizaton of the exudate metabo-lipidome in Tall Wheatgrass bioRxiv. Plant Biol. Pub Date : 2024-03-25 Sneha P Couvillion, Isabella Yang, Josie Eder, Sheryl Bell, Kirsten Hofmockel
The rhizosphere, where plant roots meet soil, is a hub of biogeochemical activity with ecosystem impacts on carbon stocks. Root derived carbon has been found to contribute more to soil carbon stocks than aboveground litter, but the molecular chemodiversity of root exudates remains poorly understood. Here our goal was to discover the molecular chemodiversity of metabolites and lipids in root exudates
-
Root foraging response to gradients of calcium and magnesium, essential bivalent cations with low mobility in the soil bioRxiv. Plant Biol. Pub Date : 2024-03-25 Hana Skalova, Karolina Pankova, Pavlina Stiblikova, Filip Krivohlavy, Veroslava Hadincova, Edita Tylova, Tomas Herben
Plants forage for nutrients by root proliferation in nutrient-rich patches. While foraging for nitrogen and phosphorus has been repeatedly confirmed, foraging for calcium and magnesium, which are essential for plant growth and form much more stable patches in the soil, has never been examined. We examined preferential root placement into dolomite-limestone-rich patches in a pot experiment with 17 species
-
A circadian transcriptional sub-network and EARLY FLOWERING 3 control timing of senescence and grain nutrition in bread wheat bioRxiv. Plant Biol. Pub Date : 2024-03-25 Christopher R Buckley, Joshua M Boyte, Robert L Albiston, Jessica Hyles, Jesse T Beasley, Alexander AT Johnson, Ben Trevaskis, Alexandre Fournier-Level, Michael J Haydon
Circadian clocks control daily and seasonal timing of physiology and development. Because of their influence on photoperiodic flowering, variants in circadian clock genes have been selected for phenology during domestication of cereal crops. To explore the potential impact of this genetic variation on circadian-regulated traits, we investigated the relationship of the circadian clock and leaf senescence
-
Phylotranscriptomics provides a treasure trove of flood tolerance mechanisms in the Cardamineae tribe bioRxiv. Plant Biol. Pub Date : 2024-03-25 Hans van Veen, Jana T Mueller, Malte M Bartylla, Melis Akman, Rashmi Sasidharan, Angelika Mustroph
Flooding events are highly detrimental to most terrestrial plant species. However, there is an impressive diversity of plant species that thrive in flood-prone regions and represent a treasure trove of unexplored flood-resilience mechanisms. Here we surveyed a panel of four species from the Cardamineae tribe representing a broad tolerance range. This included the flood-tolerant Cardamine pratensis
-
Effects of nano urea on growth and gene expression of Arabidopsis thaliana in hydroponics bioRxiv. Plant Biol. Pub Date : 2024-03-25 NEELAM JANGID, DEBANKONA MARIK, DEVANSHU VERMA, ARPAN DEY, RAJVEER SINGH SHEKHAWAT, DEEP PATEL, PANKAJ YADAV, KIRTI SANKHALA, AYAN SADHUKHAN
Introduction: Hydroponics enables precise control over nutrient delivery, optimized water requirements and growing conditions. The combination of nanotechnology and hydroponics paves the way towards sustainable agriculture with less environmental footprints. We investigated the effects of nano urea on the model plant Arabidopsis thaliana in hydroponics. Methods: A growth experiment in a nitrogen-free
-
HyperPRI: A Dataset of Hyperspectral Images for Underground Plant Root Study bioRxiv. Plant Biol. Pub Date : 2024-03-25 Spencer J Chang, Ritesh Chowdhry, Yangyang Song, Tomas Mejia, Anna Hampton, Shelby Kucharski, TM Sazzad, Yuxuan Zhang, Sanjeev J Koppal, Chris H Wilson, Stefan Gerber, Barry Tillman, Marcio FR Resende, William M Hammond, Alina Zare
Collecting and analyzing hyperspectral imagery (HSI) of plant roots over time can enhance our understanding of their function, responses to environmental factors, turnover, and relationship with the rhizosphere. Current belowground red-green-blue (RGB) root imaging studies infer such functions from physical properties like root length, volume, and surface area. HSI provides a more complete spectral
-
On the mechanism for winter stem pressure build-up in walnut trees bioRxiv. Plant Biol. Pub Date : 2024-03-25 Cyril Bozonnet, Marc Saudreau, Eric Badel, Guillaume Charrier, Thierry Ameglio
Xylem embolism is a significant factor in tree mortality. Restoration of hydraulic conductivity after massive embolisation of the vascular system requires the application of positive pressure to the vessels and/or the creation of new conductive elements. Some species generate positive pressure from the root system to propagate pressure in distal, aboveground, organs in spring, whereas other species
-
NRC immune receptor networks show diversified hierarchical genetic architecture across plant lineages bioRxiv. Plant Biol. Pub Date : 2024-03-24 Foong-Jing Goh, Ching-Yi Huang, Lida Derevnina, Chih-Hang Wu
Plants developed sophisticated immune systems consisting of nucleotide-binding domain and leucine-rich repeat-containing (NLR) proteins to repel invading pathogens. The NRC (NLR required for cell death) family is a group of helper NLRs that form a complex genetic network with multiple sensor NLRs to provide resistance against various pathogens of solanaceous plants. However, how the NRC network has
-
SUC2 sucrose transporter is required for leaf apoplasmic sucrose levels. Consequences for phloem loading strategies bioRxiv. Plant Biol. Pub Date : 2024-03-23 Francoise Vilaine, Laurence Bill, Rozenn Le Hir, Catherine Bellini, Sylvie Dinant
The SUC/SUT sucrose transporters belong to a family of active H+/sucrose symporters, with a role of SUC2 in active apoplasmic phloem loading to drive long-distance phloem transport of sucrose in Arabidopsis. However, the cooperation with the symplasmic pathway for phloem loading remains unclear. In this study, we explored the consequences of reducing either apoplasmic or symplasmic pathways of phloem
-
A frosty genetic screen unmasks a major regulatory role for SHORT VEGETATIVE PHASE of flowering in response to a cold snap bioRxiv. Plant Biol. Pub Date : 2024-03-23 Ashleigh Edwards, Hans Thordal-Christensen, Stephan Wenkel
The control of flowering in plants is intricately governed by a combination of internal and environmental signals, with temperature playing a critical role. Thus, Arabidopsis thaliana plants display temperature-dependent variations in flowering time. As unexpected periods of cold temperatures can occur at any time, plants have evolved mechanisms to detect such cold snaps and to respond by delaying
-
A Comparative Study on the Superiority of AOS DP3-7 Over 5-ALA in Facilitating Pigment Change in Peppers bioRxiv. Plant Biol. Pub Date : 2024-03-23 Xingqiang Chen, Haidong Chen, Zheng Shang
This study investigates the impact of Alginate Oligosaccharides (AOS) and 5-Aminolevulinic Acid (5-ALA) on the maturation process of red peppers, focusing on color transformation, weight gain, seed density, and skin thickness. Treatments included foliar applications of 100 ppm solutions of AOS and 5-ALA, compared with a control group, over a period of two weeks in a controlled environment. Results
-
Both major xanthophyll cycles present in nature can provide Non-Photochemical Quenching in the model diatom Phaeodactylum tricornutum bioRxiv. Plant Biol. Pub Date : 2024-03-22 Chiara E Giossi, Marie A Wünsch, Oliver Dautermann, Alexander F Schober, Jochen M Buck, Peter G Kroth, Martin Lohr, Bernard Lepetit
Photosynthetic organisms require light but also rely on photoprotection to preempt photodamage induced by excess light. The xanthophyll cycle, a light-dependent carotenoid interconversion, plays a key role in the on- and offset of Non-Photochemical Quenching (NPQ), a form of excess energy dissipation through heat. While in most photosynthetic eukaryotes, including brown algae, green algae and plants
-
Dual action of sphinganine in the plant disease resistance to bacteria. bioRxiv. Plant Biol. Pub Date : 2024-03-22 Eloise Huby, Sandra Villaume, Catherine Chemotti, Stephan Dorey, Sylvain Cordelier, Jerome Crouzet, Guillaume Gilliard, Christine Terryn, Alexandre Berquand, Cornelia Herrfurth, Ivo Feussner, Cedric Jacquard, Florence Fontaine, Christophe Clement, Fabienne Baillieul, Magali Deleu, Sandrine Dhondt-Cordelier
Sphingolipids are ubiquitous, highly diverse molecules constituting at least 40% of plant plasma membranes. Initially known as modulators of membrane integrity, they now emerge as important players in plant responses to (a)biotic stresses. The interaction between Arabidopsis thaliana and the bacterium Pseudomonas syringae pv. tomato DC3000 AvrRpm1 (Pst AvrRpm1) culminates in the activation of a programmed
-
Two structurally different oomycete lipophilic MAMPs induce distinctive plant immune responses. bioRxiv. Plant Biol. Pub Date : 2024-03-22 Mohammad Shahjahan Monjil, Hiroaki Kato, Satomi Ota, Kentaro Matsuda, Natsumi Suzuki, Shiho Tenhiro, Ayane Tatsumi, Sreynich Pring, Atsushi Miura, Maurizio Camagna, Takamasa Suzuki, Aiko Tanaka, Ryohei Terauchi, Ikuo Sato, Sotaro Chiba, Kazuhito Kawakita, Makoto Ojika, Daigo Takemoto
Plants recognize a variety of external signals and induce appropriate mechanisms to increase their tolerance to biotic and abiotic stresses. Precise recognition of attacking pathogens and induction of effective resistance mechanisms are critical functions for plant survival. Some molecular patterns unique to a certain group of microbes, microbe-associated molecular patterns (MAMPs), are sensed by plant
-
Epigenetic and transcriptional consequences of chemically induced transposon mobilization in the endosperm bioRxiv. Plant Biol. Pub Date : 2024-03-21 Gerardo del Toro-de León, Joram van Boven, Juan Santos-González, Wen-Biao Jiao, Korbinian Schneeberger, Claudia Köhler
Genomic imprinting, an epigenetic phenomenon leading to parent-of-origin-specific gene expression, has independently evolved in the endosperm of flowering plants and the placenta of mammals — tissues crucial for nurturing embryos. While transposable elements (TEs) frequently colocalize with imprinted genes and are implicated in imprinting establishment, direct investigations of the impact of de novo
-
Exploring the role of cultivar, year and plot age in the incidence of grapevine trunk diseases: insights from 20 years of regional surveys in France bioRxiv. Plant Biol. Pub Date : 2024-03-21 Lucas Etienne, Frederic Fabre, Davide Martinetti, Elise Frank, Lucie Michel, Valerie Bonnardot, Lucia Guerin Dubrana, Chloe E.L. Delmas
Grapevine trunk diseases cause yield losses and vine mortality in vineyards worldwide. However, there have been few quantitative studies evaluating grapevine dieback on a large spatial and temporal scale. Here, we consolidated and standardised databases from the 13 main wine regions of France, compiling records of foliar symptoms associated with esca and Eutypa dieback from 2082 plots and 36 cultivars
-
Separate domains of the Arabidopsis ENHANCER OF PINOID drive its own polarization and recruit PIN1 to the plasma membrane bioRxiv. Plant Biol. Pub Date : 2024-03-21 Michaela Matthes, Nicole Yun, Miriam Luichtl, Ulrich Büschges, Birgit Fiesselmann, Benjamin Strickland, Marietta Lehnardt, Ramon Torres
The Arabidopsis ENHANCER OF PINOID (ENP) protein and the AGC-kinase PINOID (PID) synergistically impact on polarization of the auxin transporter PIN-FORMED1 (PIN1) required for plant leaf and flower organ development. ENP offers a PID-independent input for PIN-polarity since enp pid double mutants lead to cotyledon- and flower-less plants in contrast to pid single mutants, which develop cotyledons
-
Arabidopsis uses a molecular grounding mechanism and a biophysical circuit breaker to limit floral abscission signaling bioRxiv. Plant Biol. Pub Date : 2024-03-21 Isaiah W. Taylor, O. Rahul Patharkar, Che-Wei Hsu, John Baer, Medha Mijar, Chad E. Niederhuth, Uwe Ohler, Philip N. Benfey, John C. Walker
Abscission is the programmed separation of plant organs. It is widespread in the plant kingdom with important functions in development and environmental response. In Arabidopsis, abscission of floral organs (sepals, petals, and stamens) is controlled by two receptor-like protein kinases HAESA (HAE) and HAESA LIKE-2 (HSL2), which orchestrate the programmed dissolution of the abscission zone connecting
-
Seaweed Amino Acid and L-Amino Acid Improve Coriander Growth bioRxiv. Plant Biol. Pub Date : 2024-03-20 Xingqiang Chen, Zheng Shang, Haidong Chen, Shulin Wan
This study investigates the impact of Seaweed amino acid (SG) and L-amino acid (LG) treatments on the growth and root development of coriander plants compared to a control group (CG). The results from Figure 1 illustrate a significant increase in biomass and foliage density for the SG and LG groups, suggesting an enhanced nutritional uptake resulting from these amino acid treatments. Both SG and LG
-
Manipulating plant development by editing histone methylation with the dCas9 tool: the CUC3 boundary gene as a case study bioRxiv. Plant Biol. Pub Date : 2024-03-20 Kateryna Fal, Marie Le Masson, Alexandre Berr, Cristel C CARLES
Chromatin modifications are deemed to associate with gene expression patterns, yet their causal function on transcription and cell fate remains unestablished. Here, we demonstrate the direct impact of an epigenome editing tool designed to remove a key chromatin modification at a precise locus in living plants, with outcomes from the molecular to the developmental scale. The manipulated mark, H3K27me3
-
Molecular Phylogeny and Cryptic Morphology: A Combined Approach to Taxonomic Novelties in Polycarpaea (Caryophyllaceae) from Vietnam bioRxiv. Plant Biol. Pub Date : 2024-03-20 Tien Van Tran, Truong Thanh Hoang, Le Ba Le, Ngan Thi Kim Le, Minh Thi Ai Nguyen, Anh Thi Lan Truong, Nhung Thi Tran, Vinh Thai Tran, Son Van Le, Kim Thi Duong, Khoa Viet Bach Hoang, Trieu Ngoc Le, Binh Van Nguyen
Three new species of Polycarpaea from Vietnam, P. vanana, P. chungana, P. duongana are described and illustrated based on evidence of molecular sequence data from two markers (ITS1-5.8S-ITS2 and rps16) and combined morphological characteristics. Polycarpaea vanana is closely related to Polycarpaea gaudichaudi, P. arenaria, P. duongana but differs by its stem glabrous, leaf ovate to elliptic, glabrous
-
Identifying leaf anatomy and metabolic regulators that underpin C4 photosynthesis in Alloteropsis semialata bioRxiv. Plant Biol. Pub Date : 2024-03-20 Ahmed S Alenazi, Lara Pereira, Pascal-Antoine Christin, Colin P Osborne, Luke T Dunning
C4 photosynthesis is a complex trait requiring multiple developmental and metabolic alterations. Despite this complexity, it has independently evolved over 60 times. However, our understanding of the transition to C4 is complicated by the fact that variation in photosynthetic type is usually segregated between species. Here, we perform a genome wide association study (GWAS) using the grass Alloteropsis
-
Strong heterologous electron sink outcompetes alternative electron transport pathways in photosynthesis bioRxiv. Plant Biol. Pub Date : 2024-03-20 Michal Hubacek, Laura T Wey, Robert Kourist, Lenny Malihan-Yap, Lauri Nikkanen, Yagut Allahverdiyeva
Improvement of photosynthesis requires a thorough understanding of electron partitioning under both natural and strong electron sink conditions. We applied a wide array of state-of-the-art biophysical and biochemical techniques to thoroughly investigate the fate of photosynthetic electrons in the engineered cyanobacterium Synechocystis sp. PCC 6803, a blueprint for photosynthetic biotechnology, expressing
-
Shedding light on blue-green photosynthesis: A wavelength-dependent mathematical model of photosynthesis in Synechocystis sp. PCC 6803 bioRxiv. Plant Biol. Pub Date : 2024-03-20 Tobias Pfennig, Elena Kullmann, Tomáš Zavřel, Andreas Nakielski, Oliver Ebenhöh, Jan Červený, Gábor Bernát, Anna Barbara Matuszyńska
Cyanobacteria hold great potential to revolutionize conventional industries and farming practices with their light-driven chemical production. To fully exploit their photosynthetic capacity and enhance product yield, it is crucial to investigate their intricate interplay with the environment including the light intensity and spectrum. Mathematical models provide valuable insights for optimizing strategies
-
Grapevine Shiraz Disease-associated viruses lead to significant yield losses by altering transcription of genes related to defence responses and photosynthesis bioRxiv. Plant Biol. Pub Date : 2024-03-19 Dilrukshi Nagahatenna, Cristobal A Onetto, Yeniu Mickey Wang, Anthony R Borneman, Vinay V Pagay
Grapevine Shiraz disease (SD), which is associated with Grapevine Virus A (GVA), is one of the highly destructive diseases affecting Australian and South African vineyards. However, virtually nothing is known about the transcriptional modifications in grapevine phloem tissues induced by SD as well as its impact on vine physiology, yield and fruit composition. In this study, we assessed the physiological
-
Investigating the interactions of the cucumber mosaic virus 2b protein with the viral 1a replicase component and the cellular RNA silencing factor Argonaute 1 bioRxiv. Plant Biol. Pub Date : 2024-03-19 Sam Crawshaw, Alex M Murphy, Pamela J.E. Rowling, Daniel Nietlispach, Laura Susan Itzhaki, John P Carr
The cucumber mosaic virus (CMV) 2b protein is a suppressor of plant defenses and a pathogenicity determinant. Amongst the host targets of the 2b protein is the RNA silencing factor Argonaute 1 (AGO1), which it binds to and inhibits. In Arabidopsis thaliana, if 2b-induced inhibition of AGO1 is too efficient it induces reinforcement of antiviral silencing by AGO2, and triggers increased resistance against
-
NONEXPRESSOR OF PATHOGENESIS-RELATED GENES control Huanglongbing tolerance by regulating immune balance in citrus plants bioRxiv. Plant Biol. Pub Date : 2024-03-19 Poulami Sarkar, Choaa El-Mohtar, Donielle Turner, Stacy Welker, Cecile J Robertson, Vladimir Orbovic, Zhonglin Mou, Amit Levy
Huanglongbing (HLB) is a devastating citrus disease caused by the phloem-resident bacterial pathogen Candidatus liberibacter asiaticus (CLas). CLas infection of susceptible varieties triggers unbalanced immune responses, leading to overaccumulation of callose and reactive oxygen species (ROS), which in turn causes phloem plugging and HLB symptom development. Interestingly, some citrus relatives exhibit
-
Optimized dCas9 Transcription Activators for Plants bioRxiv. Plant Biol. Pub Date : 2024-03-19 Matthew Zinselmeier, J Armando Casas-Mollano, Jonathan Cors, Adam Sychla, Stephen Heinsch, Daniel Voytas, Michael Smanski
Understanding how the expression of genes impacts plant development and physiology is important for rationally engineering crop improvements. Programmable Transcription Activators (PTAs), including CRISPRa activators, have traditionally relied on a limited number of transcription activation domains, namely the VP64 domain derived from human herpes simplex virus, to control gene expression. We reasoned
-
Trading acyls and swapping sugars: metabolic innovations in Solanum trichomes bioRxiv. Plant Biol. Pub Date : 2024-03-19 Paul D. Fiesel, Rachel A. Kerwin, A. Daniel Jones, Robert L. Last
Solanaceae (nightshade family) species synthesize a remarkable array of clade- and tissue-specific specialized metabolites. Protective acylsugars, one such class of structurally diverse metabolites, are produced by AcylSugar AcylTransferases from sugars and acyl-coenzyme A esters. Published research revealed trichome acylsugars composed of glucose and sucrose cores in species across the family. In
-
The Chloroplast RNA Binding Protein CP29A supports rbcL expression during cold acclimation bioRxiv. Plant Biol. Pub Date : 2024-03-18 Benjamin Lenzen, Florian Roesch, Hannes Ruwe, Nitin Kachariya, Julia Legen, Michael Sattler, Ian D. Small, Christian Schmitz-Linneweber
The chloroplast genome encodes key components of the photosynthetic light reaction machinery as well as the large subunit of the enzyme central for carbon fixation, RuBisCo. Its expression is predominantly regulated post-transcriptionally, with nuclear-encoded RNA binding proteins (RBPs) playing a key role. Mutants of chloroplast gene expression factors often exhibit impaired chloroplast biogenesis
-
Chloroplastic ascorbate acts as a regulatory hub in plant metabolism regardless of oxidative stress bioRxiv. Plant Biol. Pub Date : 2024-03-18 David Toth, Roland Tengolics, Fayezeh Aarabi, Anna Karlsson, Andre Vidal-Meireles, Laszlo Kovacs, Soujanya Kuntam, Timea Kormoczi, Alisdair R. Fernie, Elton Hudson, Balazs Papp, Szilvia Z. Toth
Ascorbate is a major plant metabolite that plays crucial roles in various processes, from reactive oxygen scavenging to epigenetic regulation. However, to what extent and how ascorbate modulates metabolism is largely unknown. To address this, we investigated the consequences of chloroplastic and total cellular ascorbate-deficiencies by studying chloroplastic ascorbate-transporter pht4;4 mutant lines
-
Discovery of active mouse, plant and fungal cytochrome P450s in endogenous proteomes and upon expression in planta. bioRxiv. Plant Biol. Pub Date : 2024-03-18 Maria Font Farre, Daniel Brown, Reka Toth, Chidambareswaren Mahadevan, Melissa Brazier-Hicks, Kyoko Morimoto, Farnusch Kaschani, John Sinclair, Richard Dale, Samantha Hall, Melloney Morris, Markus Kaiser, Aaron T. Wright, Jonathan Burton, Renier A. L. van der Hoorn
Eukaryotes produce a large number of cytochrome P450s that mediate the synthesis and degradation of diverse endogenous and exogenous metabolites. Yet, most of these P450s are uncharacterized and global tools to study these challenging, membrane-resident enzymes remain to be exploited. Here, we applied activity profiling of plant, mouse and fungal P450s with chemical probes that become reactive when
-
High resolution mapping of novel non-transgressive hybrid susceptibility in barley exploited by P. teres f. maculata maps to a single pentatricopeptide repeat-containing protein bioRxiv. Plant Biol. Pub Date : 2024-03-17 Shaun James Clare, Abdullah F Alhashel, Mengyuan Li, Karl M Effertz, Roshan Sharma Poudel, Jianwei Zhang, Robert Saxon Brueggeman
Hybrid genotypes can provide significant yield gains over conventional inbred varieties due to heterosis or hybrid vigor. However, hybrids can also display unintended negative attributes or phenotypes such as extreme pathogen susceptibility. The necrotrophic pathogen Pyrenophora teres f. maculata (Ptm) causes spot form net blotch, which has caused significant losses to barley worldwide. Here, we report
-
Effects of arbuscular mycorrhizal fungi on nitrogen uptake in cotton (Gossypium hirsutum L.) under low-nitrogen conditions bioRxiv. Plant Biol. Pub Date : 2024-03-17 Hushan Wang, yijian wang, Xiaojiao cheng, Yunzhu He, zihui shen, Wangfeng zhang, xiaozhen pu
Cotton is an important global cash crop whose yield and quality are highly influenced by soil nitrogen. Therefore, examining the interactions between roots and arbuscular mycorrhizal fungi (AMF) under reduced nitrogen conditions is of great significance. We investigated the effects of nitrogen application (0, 250, and 375 kg*hm-2) on the AMF infection rate of cotton, the nitrogen content of each organ
-
The distribution of particulate organic matter in the heterogeneous soil matrix - balancing between aerobic respiration and denitrification bioRxiv. Plant Biol. Pub Date : 2024-03-17 Maik Lucas, Lena Rohe, Bernd Apelt, Claus Florian Stange, Hans-Jörg Vogel, Reinhard Well, Steffen Schlüter
Denitrification, a key process in soil nitrogen cycling, occurs predominantly within microbial hotspots, where denitrifiers use nitrate as an alternative electron acceptor. For accurate prediction of dinitrogen (N2) and nitrous oxide (N2O) emissions from denitrification, a precise quantification of these microscale hotspots is required. Employing a unique combination of X-ray CT imaging, microscale