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Dynamic pathological analysis reveals a protective role against skin fibrosis for TREM2-dependent macrophages Theranostics (IF 12.4) Pub Date : 2024-3-11 Yunsheng Liang, Yongfei Hu, Jun Zhang, Haosen Song, Xiaoqian Zhang, Yishan Chen, Yu Peng, Lihua Sun, Yuzhe Sun, Ruzeng Xue, Suyun Ji, Chuanwei Li, Zhili Rong, Bin Yang, Yingping Xu
Rationale: Systemic sclerosis (SSc) is a chronic and incurable autoimmune disease with high mortality rates, and skin fibrosis is one of distinguishing hallmarks in the pathogenesis. However, macrophage heterogeneity regulating skin fibrosis remain largely unknown./nMethods: We established mouse disease model and performed single-cell RNA-sequencing (scRNA-seq) to resolve the dynamic and heterogenous
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Myocardin reverses insulin resistance and ameliorates cardiomyopathy by increasing IRS-1 expression in a murine model of lipodystrophy caused by adipose deficiency of vacuolar H+-ATPase V0d1 subunit Theranostics (IF 12.4) Pub Date : 2024-3-11 Wenlin Yuan, Hui Lin, Yuan Sun, Lihuan Liu, Meijuan Yan, Yujuan Song, Xiaofan Zhang, Xiangling Lu, Yipei Xu, Qiyue He, Kunfu Ouyang, Chenglin Zhang, Yong Pan, Yu Huang, Ying Li, Xifeng Lu, Jie Liu
Aim: Adipose tissue (AT) dysfunction that occurs in both obesity and lipodystrophy is associated with the development of cardiomyopathy. However, it is unclear how dysfunctional AT induces cardiomyopathy due to limited animal models available. We have identified vacuolar H+-ATPase subunit Vod1, encoded by Atp6v0d1, as a master regulator of adipogenesis, and adipose-specific deletion of Atp6v0d1 (Atp6v0d1AKO)
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Extracellular vesicles as carriers of mRNA: Opportunities and challenges in diagnosis and treatment Theranostics (IF 12.4) Pub Date : 2024-3-11 Robert D. Kirian, Darby Steinman, Christopher M. Jewell, Hannah C. Zierden
Extracellular vesicles (EVs) are produced by all cells in the body. These biological nanoparticles facilitate cellular communication through the transport of diverse cargoes, including small molecules, proteins, and nucleic acids. mRNA cargoes have gained particular interest given their role in the translation of functional proteins. As a biomarker platform, EVs can be found in nearly all biofluids—blood
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An advanced comprehensive muti-cell-type-specific model for predicting anti-PD-1 therapeutic effect in melanoma Theranostics (IF 12.4) Pub Date : 2024-3-3 Wei Sun, Yu Zhu, Zijian Zou, Lu Wang, Jingqin Zhong, Kangjie Shen, Xinyi Lin, Zixu Gao, Wanlin Liu, Yinlam Li, Yu Xu, Ming Ren, Tu Hu, Chuanyuan Wei, Jianying Gu, Yong Chen
Rationale: Immune checkpoint inhibitors targeting the programmed cell death (PD)-1/PD-L1 pathway have promise in patients with advanced melanoma. However, drug resistance usually results in limited patient benefits. Recent single-cell RNA sequencing studies have elucidated that MM patients display distinctive transcriptional features of tumor cells, immune cells and interstitial cells, including loss
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FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation Theranostics (IF 12.4) Pub Date : 2024-3-3 Xingyan Xu, Xuefen Zhuang, Haowei Yu, Ping Li, Xiaosa Li, Huiping Lin, Jian-peng Teoh, Yiwen Chen, Yuanlan Yang, Yang Cheng, Weiyu Chen, Xiaodong Fu
Background: The therapeutic benefits of targeting follicle-stimulating hormone (FSH) receptor in treatment of ovarian cancer are significant, whereas the role of FSH in ovarian cancer progresses and the underlying mechanism remains to be developed./nMethods: Tissue microarray of human ovarian cancer, tumor xenograft mouse model, and in vitro cell culture were used to investigate the role of FSH in
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Immunotherapeutic IL-6R and targeting the MCT-1/IL-6/CXCL7/PD-L1 circuit prevent relapse and metastasis of triple-negative breast cancer Theranostics (IF 12.4) Pub Date : 2024-3-3 Aushia Tanzih Al Haq, Pao-Pao Yang, Christopher Jin, Jou-Ho Shih, Li-Mei Chen, Hong-Yu Tseng, Yen-An Chen, Yueh-Shan Weng, Lu-Hai Wang, Michael P. Snyder, Hsin-Ling Hsu
Rationale: Multiple copies in T-cell malignancy 1 (MCT-1) is a prognostic biomarker for aggressive breast cancers. Overexpressed MCT-1 stimulates the IL-6/IL-6R/gp130/STAT3 axis, which promotes epithelial-to-mesenchymal transition and cancer stemness. Because cancer stemness largely contributes to the tumor metastasis and recurrence, we aimed to identify whether the blockade of MCT-1 and IL-6R can
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G protein subunit alpha i2's pivotal role in angiogenesis Theranostics (IF 12.4) Pub Date : 2024-3-3 Chao-wen Bai, Lu Lu, Jia-nan Zhang, Chengyu Zhou, Yi-chao Ni, Ke-ran Li, Jin Yao, Xiao-zhong Zhou, Chang-gong Lan, Cong Cao
Here we explored the potential role of Gαi2 (G protein subunit alpha i2) in endothelial cell function and angiogenesis./nMethods: Genetic methodologies such as shRNA, CRISPR/Cas9, dominant negative mutation, and overexpression were utilized to modify Gαi2 expression or regulate its function. Their effects on endothelial cell functions were assessed in vitro. In vivo, the endothelial-specific Gαi2 shRNA
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Intravital imaging of splenic classical monocytes modifying the hepatic CX3CR1+ cells motility to exacerbate liver fibrosis via spleen-liver axis Theranostics (IF 12.4) Pub Date : 2024-3-3 Chenlu Han, Yujie Zhai, Yuke Wang, Xuwen Peng, Xian Zhang, Bolei Dai, Yuehong Leng, Zhihong Zhang, Shuhong Qi
CX3CR1+ cells play a crucial role in liver fibrosis progression. However, changes in the migratory behavior and spatial distribution of spleen-derived and hepatic CX3CR1+ cells in the fibrotic liver as well as their influence on the liver fibrosis remain unclear./nMethods: The CX3CR1GFP/+ transgenic mice and CX3CR1-KikGR transgenic mice were used to establish the CCl4-induced liver fibrosis model.
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Engineered exosomes and composite biomaterials for tissue regeneration Theranostics (IF 12.4) Pub Date : 2024-3-3 Weikang Hu, Wang Wang, Zesheng Chen, Yun Chen, Zijian Wang
Exosomes, which are small vesicles enclosed by a lipid bilayer and released by many cell types, are widely dispersed and have garnered increased attention in the field of regenerative medicine due to their ability to serve as indicators of diseases and agents with therapeutic potential. Exosomes play a crucial role in mediating intercellular communication through the transfer of many biomolecules,
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Unleashing the potential of adipose organoids: A revolutionary approach to combat obesity-related metabolic diseases Theranostics (IF 12.4) Pub Date : 2024-2-25 Xingran Liu, Jing Yang, Yuxin Yan, Qingfeng Li, Ru-Lin Huang
Obesity-related metabolic diseases, including obesity, diabetes, hyperlipidemia, and non-alcoholic fatty liver diseases pose a significant threat to health. However, comprehensive pathogenesis exploration and effective therapy development are impeded by the limited availability of human models. Notably, advances in organoid technology enable the generation of adipose organoids that recapitulate structures
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Precision peptide theranostics: developing N- to C-terminus optimized theranostics targeting cholecystokinin-2 receptor Theranostics (IF 12.4) Pub Date : 2024-2-24 Marwa N. Rahimi, Alicia Corlett, Jessica Van Zuylekom, Marc Antoine Sani, Benjamin Blyth, Philip Thompson, Peter D. Roselt, Mohammad B. Haskali
Peptides are ideal for theranostic development as they afford rapid target accumulation, fast clearance from background tissue, and exhibit good tissue penetration. Previously, we developed a novel series of peptides that presented discreet folding propensity leading to an optimal candidate [68Ga]Ga-DOTA-GA1 ([D-Glu]6-Ala-Tyr-NMeGly-Trp-NMeNle-Asp-Nal-NH2) with 50 pM binding affinity against cholecystokinin-2
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[161Tb]Tb-PSMA-617 radioligand therapy in patients with mCRPC: preliminary dosimetry results and intra-individual head-to-head comparison to [177Lu]Lu-PSMA-617 Theranostics (IF 12.4) Pub Date : 2024-2-24 Andrea Schaefer-Schuler, Caroline Burgard, Arne Blickle, Stephan Maus, Christine Petrescu, Sven Petto, Mark Bartholomä, Tobias Stemler, Samer Ezziddin, Florian Rosar
Rationale: Evaluation of alternative radionuclides for use in prostate-specific membrane antigen (PSMA)-targeted radioligand therapy (RLT) is currently focusing on 161Tb, which may provide advantages by emitting additional Auger and conversion electrons. In this pilot study, we present preliminary dosimetry data for [161Tb]Tb-PSMA-617 RLT in a direct comparison with [177Lu]Lu-PSMA-617./nMethod: Six
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Dual regulation of NEMO by Nrf2 and miR-125a inhibits ferroptosis and protects liver from endoplasmic reticulum stress-induced injury Theranostics (IF 12.4) Pub Date : 2024-2-24 Jihoon Tak, Min Sung Joo, Yun Seok Kim, Hyun Woo Park, Chang Hoon Lee, Gil-Chun Park, Shin Hwang, Sang Geon Kim
Rationale: The surge of severe liver damage underscores the necessity for identifying new targets and therapeutic agents. Endoplasmic reticulum (ER) stress induces ferroptosis with Gα12 overexpression. NF-κB essential modulator (NEMO) is a regulator of inflammation and necroptosis. Nonetheless, the regulatory basis of NEMO de novo synthesis and its impact on hepatocyte ferroptosis need to be established
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Site-specific albumin tagging with NIR-II fluorogenic dye for high-performance and super-stable bioimaging Theranostics (IF 12.4) Pub Date : 2024-2-24 Ningning Zhu, Jiajun Xu, Qi Su, Tianyang Han, Ding Zhou, Yuewei Zhang, Shoujun Zhu
Synthetic near-infrared-II (NIR-II) dyes are promising for deep tissue imaging, yet they are generally difficult to target a given biomolecule with high specificity. Furthermore, the interaction mechanism between albumin and cyanine molecules, which is usually regarded as uncertain "complexes" such as crosslinked nanoparticles, remains poorly understood./nMethods: Here, we propose a new class of NIR-II
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Cancer-associated fibroblast spatial heterogeneity and EMILIN1 expression in the tumor microenvironment modulate TGF-β activity and CD8+ T-cell infiltration in breast cancer Theranostics (IF 12.4) Pub Date : 2024-2-24 Chikako Kanno Honda, Sasagu Kurozumi, Takaaki Fujii, Didier Pourquier, Lakhdar Khellaf, Florence Boissiere, Jun Horiguchi, Tetsunari Oyama, Ken Shirabe, Jacques Colinge, Takehiko Yokobori, Andrei Turtoi
Rationale: The tumor microenvironment (TME) and its multifaceted interactions with cancer cells are major targets for cancer treatment. Single-cell technologies have brought major insights into the TME, but the resulting complexity often precludes conclusions on function./nMethods: We combined single-cell RNA sequencing and spatial transcriptomic data to explore the relationship between different cancer-associated
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Fibroblasts facilitate lymphatic vessel formation in transplanted heart Theranostics (IF 12.4) Pub Date : 2024-2-24 Hui Gong, Ting Wang, Xiaotong Sun, Yuesheng Zhang, Yichao Qiu, Wenlong Sun, Yue Zhang, Peng Teng, Yanhua Hu, Xiaosheng Hu, Liang Ma, Qingbo Xu, Haige Zhao
Rationale: Lymphangiogenesis plays a critical role in the transplanted heart. The remodeling of lymphatics in the transplanted heart and the source of newly formed lymphatic vessels are still controversial, especially the mechanism of lymphangiogenesis remains limited./nMethods: Heart transplantation was performed among BALB/c, C57BL/6J, Cag-Cre, Lyve1-CreERT2;Rosa26-tdTomato and Postn(2A-CreERT2-
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Neutrophil extracellular trap-mediated impairment of meningeal lymphatic drainage exacerbates secondary hydrocephalus after intraventricular hemorrhage Theranostics (IF 12.4) Pub Date : 2024-2-24 Qiang Zhang, Yujie Chen, Yingpei Li, Zhou Feng, Liang Liang, Xiaoke Hao, Wenbo Kang, Zhaoqi Zhang, Xuyang Zhang, Rong Hu, Hua Feng, Zhi Chen
Rationale: Hydrocephalus is a substantial complication after intracerebral hemorrhage (ICH) or intraventricular hemorrhage (IVH) that leads to impaired cerebrospinal fluid (CSF) circulation. Recently, brain meningeal lymphatic vessels (mLVs) were shown to serve as critical drainage pathways for CSF. Our previous studies indicated that the degradation of neutrophil extracellular traps (NETs) after ICH/IVH
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Multifunctional Sr/Se co-doped ZIF-8 nanozyme for chemo/chemodynamic synergistic tumor therapy via apoptosis and ferroptosis Theranostics (IF 12.4) Pub Date : 2024-2-24 Aimin Wu, Ming Han, Zihan Ni, Haoran Li, Yinyin Chen, Zhouping Yang, Yumei Feng, Zufeng He, Hua Zhen, Xianxiang Wang
Rationale: Cancer continues to be a significant public health issue. Traditional treatments such as surgery, radiotherapy, and chemotherapy often fall short because of intrinsic issues such as lack of specificity and poor drug delivery, leading to insufficient drug concentration at the tumor site and/or potential side effects. Consequently, improving the delivery of conventional chemotherapy drugs
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H-ferritin-nanocaged gadolinium nanoparticles for ultra-sensitive MR molecular imaging Theranostics (IF 12.4) Pub Date : 2024-2-24 Jianlin Zhang, Chang Yuan, Lingfei Kong, Feiyan Zhu, Wanzhong Yuan, Junying Zhang, Juanji Hong, Fang Deng, Qi Chen, Chen Chen, Tao Wang, Zhentao Zuo, Minmin Liang
Rationale: Magnetic resonance imaging (MRI) is a powerful diagnostic technology by providing high-resolution imaging. Although MRI is sufficiently valued in its resolving morphology, it has poor sensitivity for tracking biomarkers. Therefore, contrast agents are often used to improve MRI diagnostic sensitivity. However, the clinically used Gd chelates are limited in improving MRI sensitivity owing
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Depletion of ApoA5 aggravates spontaneous and diet-induced nonalcoholic fatty liver disease by reducing hepatic NR1D1 in hamsters Theranostics (IF 12.4) Pub Date : 2024-2-24 Jiabao Guo, Guolin Miao, Wenxi Zhang, Haozhe Shi, Pingping Lai, Yitong Xu, Lianxin Zhang, Gonglie Chen, Yufei Han, Ying Zhao, Geroge Liu, Ling Zhang, Yuhui Wang, Wei Huang, Xunde Xian
Background: ApoA5 mainly synthesized and secreted by liver is a key modulator of lipoprotein lipase (LPL) activity and triglyceride-rich lipoproteins (TRLs). Although the role of ApoA5 in extrahepatic triglyceride (TG) metabolism in circulation has been well documented, the relationship between ApoA5 and nonalcoholic fatty liver disease (NAFLD) remains incompletely understood and the underlying molecular
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NPC1 is required for postnatal islet β cell differentiation by maintaining mitochondria turnover Theranostics (IF 12.4) Pub Date : 2024-2-24 Bei Liu, Duanyi Hua, Linyan Shen, Tingting Li, Zheying Tao, Chenyang Fu, Zhongzheng Tang, Jie Yang, Li Zhang, Aifang Nie, Yiran Jiang, Jiqiu Wang, Yang Li, Yanyun Gu, Guang Ning
Rationale: NPC1 is a protein localized on the lysosome membrane regulating intracellular cholesterol transportation and maintaining normal lysosome function. GWAS studies have found that NPC1 variants in T2D was a pancreatic islet expression quantitative trait locus, suggesting a potential role of NPC1 in T2D islet pathophysiology./nMethods: Two-week-old Npc1-/- mice and wild type littermates were
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Cancer diagnosis using label-free SERS-based exosome analysis Theranostics (IF 12.4) Pub Date : 2024-2-24 Yajuan Liu, Mei Li, Haisha Liu, Chao Kang, Cheng Wang
Exosomes, carrying distinctive biomolecules reflective of their parent cell's status and origin, show promise as liquid biopsy biomarkers for cancer diagnosis. However, their clinical translation remains challenging due to their relatively low concentration in body fluids. Surface-Enhanced Raman spectroscopy (SERS) has recently gained significant attention as a label-free and sensitive technique for
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Highly oriented hydrogels for tissue regeneration: design strategies, cellular mechanisms, and biomedical applications Theranostics (IF 12.4) Pub Date : 2024-2-24 Jiuping Wu, Zhihe Yun, Wenlong Song, Tao Yu, Wu Xue, Qinyi Liu, Xinzhi Sun
Many human tissues exhibit a highly oriented architecture that confers them with distinct mechanical properties, enabling adaptation to diverse and challenging environments. Hydrogels, with their water-rich "soft and wet" structure, have emerged as promising biomimetic materials in tissue engineering for repairing and replacing damaged tissues and organs. Highly oriented hydrogels can especially emulate
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In vivo high-speed microscopy of microbubbles in the chorioallantoic membrane model Theranostics (IF 12.4) Pub Date : 2024-2-17 Rojin Anbarafshan, Carly Pellow, Kevin Kiezun, Hon Leong, David E. Goertz
Rationale: The acoustic stimulation of microbubbles within microvessels can elicit a spectrum of therapeutically relevant bioeffects from permeabilization to perfusion shutdown. These bioeffects ultimately arise from complex interactions between microbubbles and microvascular walls, though such interactions are poorly understood particularly at high pressure, due to a paucity of direct in vivo observations
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BitterDB database analysis plus cell stiffness screening identify flufenamic acid as the most potent TAS2R14-based relaxant of airway smooth muscle cells for therapeutic bronchodilation Theranostics (IF 12.4) Pub Date : 2024-2-17 Kai Ni, Bo Che, Rong Gu, Chunhong Wang, Hongyang Xu, Huiduo Li, Shiyan Cen, Mingzhi Luo, Linhong Deng
Rationale: Bitter taste receptors (TAS2Rs) are abundantly expressed in airway smooth muscle cells (ASMCs), which have been recognized as promising targets for bitter agonists to initiate relaxation and thereby prevent excessive airway constriction as the main characteristic of asthma. However, due to the current lack of tested safe and potent agonists functioning at low effective concentrations, there
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Mrc1+ macrophage-derived IGF1 mitigates crystal nephropathy by promoting renal tubule cell proliferation via the AKT/Rb signaling pathway Theranostics (IF 12.4) Pub Date : 2024-2-17 Linxi Huang, Wei Chen, Zhuojing Tan, Yunxiao Huang, Xinji Gu, Lantian Liu, Hongxia Zhang, Yihan Shi, Jiarong Ding, Chengjian Zheng, Zhiyong Guo, Bing Yu
Rationale: The present understanding of the cellular characteristics and communications in crystal nephropathy is limited. Here, molecular and cellular studies combined with single-cell RNA sequencing (scRNA-seq) were performed to investigate the changes in cell components and their interactions in glyoxylate-induced crystallized kidneys to provide promising treatments for crystal nephropathy./nMethods:
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Activatable prodrug for controlled release of an antimicrobial peptide via the proteases overexpressed in Candida albicans and Porphyromonas gingivalis Theranostics (IF 12.4) Pub Date : 2024-2-17 Lubna Amer, Maurice Retout, Jesse V. Jokerst
Candida albicans and Porphyromonas gingivalis are prevalent in the subgingival area where the frequency of fungal colonization increases with periodontal disease. Candida's transition to a pathogenic state and its interaction with P. gingivalis exacerbate periodontal disease severity. However, current treatments for these infections differ, and combined therapy remains unexplored. This work is based
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Terbium radionuclides for theranostic applications in nuclear medicine: from atom to bedside Theranostics (IF 12.4) Pub Date : 2024-2-17 Camille Van Laere, Michel Koole, Christophe M. Deroose, Michiel Van de Voorde, Kristof Baete, Thomas E. Cocolios, Charlotte Duchemin, Maarten Ooms, Frederik Cleeren
Terbium features four clinically interesting radionuclides for application in nuclear medicine: terbium-149, terbium-152, terbium-155, and terbium-161. Their identical chemical properties enable the synthesis of radiopharmaceuticals with the same pharmacokinetic character, while their distinctive decay characteristics make them valuable for both imaging and therapeutic applications. In particular,
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Focused ultrasound ablation of melanoma with boiling histotripsy yields abscopal tumor control and antigen-dependent dendritic cell activation Theranostics (IF 12.4) Pub Date : 2024-2-11 Eric A. Thim, Lydia E. Kitelinger, Fátima Rivera-Escalera, Alexander S. Mathew, Michael R. Elliott, Timothy N. J. Bullock, Richard J. Price
Background: Boiling histotripsy (BH), a mechanical focused ultrasound ablation strategy, can elicit intriguing signatures of anti-tumor immunity. However, the influence of BH on dendritic cell function is unknown, compromising our ability to optimally combine BH with immunotherapies to control metastatic disease./nMethods: BH was applied using a sparse scan (1 mm spacing between sonications) protocol
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Integrated electronic/fluidic microneedle system for glucose sensing and insulin delivery Theranostics (IF 12.4) Pub Date : 2024-2-11 Xinshuo Huang, Baoming Liang, Shuang Huang, Zhengjie Liu, Chuanjie Yao, Jingbo Yang, Shantao Zheng, Feifei Wu, Wan Yue, Ji Wang, Huijiuan Chen, Xi Xie
Background: Precise and dynamic blood glucose regulation is paramount for both diagnosing and managing diabetes. Continuous glucose monitoring (CGM) coupled with insulin pumps forms an artificial pancreas, enabling closed-loop control of blood glucose levels. Indeed, this integration necessitates advanced micro-nano fabrication techniques to miniaturize and combine sensing and delivery modules on a
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Cancer-associated fibroblasts reprogram cysteine metabolism to increase tumor resistance to ferroptosis in pancreatic cancer Theranostics (IF 12.4) Pub Date : 2024-2-11 Yuchao Zhu, Shuai Fang, Bolin Fan, Kaiwei Xu, Liu Xu, Linwei Wang, Lubin Zhu, Chunqu Chen, Ruoyu Wu, Jiajing Ni, Jianhua Wang
Background: Pancreatic ductal adenocarcinoma (PDAC) is an insidious, rapidly progressing malignancy of the gastrointestinal tract. Due to its dense fibrous stroma and complex tumor microenvironment, neither of which is sensitive to radiotherapy, pancreatic adenocarcinoma is one of the malignancies with the poorest prognosis. Therefore, detailed elucidation of the inhibitory microenvironment of PDAC
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Forskolin-driven conversion of human somatic cells into induced neurons through regulation of the cAMP-CREB1-JNK signaling Theranostics (IF 12.4) Pub Date : 2024-2-11 Guodong Wang, Dandan Zhang, Liangshan Qin, Quanhui Liu, Wenkui Tang, Mingxing Liu, Fan Xu, Fen Tang, Leping Cheng, Huiming Mo, Xiang Yuan, Zhiqiang Wang, Ben Huang
Human somatic cells can be reprogrammed into neuron cell fate through regulation of a single transcription factor or application of small molecule cocktails./nMethods: Here, we report that forskolin efficiently induces the conversion of human somatic cells into induced neurons (FiNs)./nResults: A large population of neuron-like phenotype cells was observed as early as 24-36 h post-induction. There
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The DNA-dependent protein kinase catalytic subunit exacerbates endotoxemia-induced myocardial microvascular injury by disrupting the MOTS-c/JNK pathway and inducing profilin-mediated lamellipodia degradation Theranostics (IF 12.4) Pub Date : 2024-2-4 Rongjun Zou, Wanting Shi, Xing Chang, Miao Zhang, Songtao Tan, Ruibing Li, Hao Zhou, Yukun Li, Ge Wang, Weihui Lv, Xiaoping Fan
Rationale: The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) promotes pathological mitochondrial fission during septic acute kidney injury. The mitochondrial open reading frame of the 12S rRNA type-c (MOTS-c) is a mitochondria-derived peptide that exhibits anti-inflammatory properties during cardiovascular illnesses. We explored whether endotoxemia-induced myocardial microvascular injury
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MAGL protects against renal fibrosis through inhibiting tubular cell lipotoxicity Theranostics (IF 12.4) Pub Date : 2024-2-4 Shan Zhou, Xian Ling, Jielin Zhu, Ye Liang, Qijian Feng, Chao Xie, Jiemei Li, Qiyan Chen, Shuangqin Chen, Jinhua Miao, Mengyao Zhang, Zhiru Li, Weiwei Shen, Xiaolong Li, Qinyu Wu, Xiaoxu Wang, Ruiyuan Liu, Cheng Wang, Fan Fan Hou, Yaozhong Kong, Youhua Liu, Lili Zhou
Rationale: Renal fibrosis, with no therapeutic approaches, is a common pathological feature in various chronic kidney diseases (CKD). Tubular cell injury plays a pivotal role in renal fibrosis. Commonly, injured tubular cells exhibit significant lipid accumulation. However, the underlying mechanisms remain poorly understood./nMethods: 2-arachidonoylglycerol (2-AG) levels in CKD patients and CKD model
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Global metabolomics revealed deviations from the metabolic aging clock in colorectal cancer patients Theranostics (IF 12.4) Pub Date : 2024-2-4 Long Zhang, Shaobo Mo, Xiurui Zhu, C. James Chou, Bo Jin, Zhi Han, James Schilling, Weili Liao, Sheeno Thyparambil, Ruben Y. Luo, John C. Whitin, Lu Tian, Seema Nagpal, Scott R. Ceresnak, Harvey J. Cohen, Doff B. McElhinney, Karl G. Sylvester, Yangming Gong, Chen Fu, Xuefeng B. Ling, Junjie Peng
Background: Markers of aging hold promise in the context of colorectal cancer (CRC) care. Utilizing high-resolution metabolomic profiling, we can unveil distinctive age-related patterns that have the potential to predict early CRC development. Our study aims to unearth a panel of aging markers and delve into the metabolomic alterations associated with aging and CRC./nMethods: We assembled a serum cohort
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20-HETE mediated TRPV1 activation drives allokinesis via MrgprA3+ neurons in chronic dermatitis Theranostics (IF 12.4) Pub Date : 2024-2-4 Guang Yu, Pei Liu, Xiaobao Huang, Mingxin Qi, Xue Li, Weimeng Feng, Erxin Shang, Yuan Zhou, Changming Wang, Yan Yang, Chan Zhu, Fang Wang, Zongxiang Tang, Jinao Duan
Rationale: Noxious stimuli are often perceived as itchy in patients with chronic dermatitis (CD); however, itch and pain mechanisms of CD are not known./nMethods: TRPV1 involvement in CD was analyzed using a SADBE induced CD-like mouse model, and several loss- and gain-of-function mouse models. Trigeminal TRPV1 channel and MrgprA3+ neuron functions were analyzed by calcium imaging and whole-cell patch-clamp
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Emerging role of extracellular vesicles in diabetic retinopathy Theranostics (IF 12.4) Pub Date : 2024-2-4 Junyan Zhu, Jin Huang, Yaoxiang Sun, Wenrong Xu, Hui Qian
Diabetic retinopathy (DR), a complex complication of diabetes mellitus (DM), is a leading cause of adult blindness. Hyperglycemia triggers DR, resulting in microvascular damage, glial apoptosis, and neuronal degeneration. Inflammation and oxidative stress play crucial roles during this process. Current clinical treatments for DR primarily target the advanced retinal disorder but offer limited benefits
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Development of CD46 targeted alpha theranostics in prostate cancer using 134Ce/225Ac-Macropa-PEG4-YS5 Theranostics (IF 12.4) Pub Date : 2024-1-27 Kondapa Naidu Bobba, Anil P. Bidkar, Anju Wadhwa, Niranjan Meher, Suchi Drona, Alexandre M. Sorlin, Scott Bidlingmaier, Li Zhang, David M. Wilson, Emily Chan, Nancy Y. Greenland, Rahul Aggarwal, Henry F. VanBrocklin, Jiang He, Jonathan Chou, Youngho Seo, Bin Liu, Robert R. Flavell
Rationale: 225Ac, a long-lived α-emitter with a half-life of 9.92 days, has garnered significant attention as a therapeutic radionuclide when coupled with monoclonal antibodies and other targeting vectors. Nevertheless, its clinical utility has been hampered by potential off-target toxicity, a lack of optimized chelators for 225Ac, and limitations in radiolabeling methods. In a prior study evaluating
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High-content stimulated Raman histology of human breast cancer Theranostics (IF 12.4) Pub Date : 2024-1-27 Hongli Ni, Chinmayee Prabhu Dessai, Haonan Lin, Wei Wang, Shaoxiong Chen, Yuhao Yuan, Xiaowei Ge, Jianpeng Ao, Nolan Vild, Ji-Xin Cheng
Histological examination is crucial for cancer diagnosis, however, the labor-intensive sample preparation involved in the histology impedes the speed of diagnosis. Recently developed two-color stimulated Raman histology could bypass the complex tissue processing to generates result close to hematoxylin and eosin staining, which is one of the golden standards in cancer histology. Yet, the underlying
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Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction Theranostics (IF 12.4) Pub Date : 2024-1-27 Meng Gao, Tuo Zhang, Tengxiang Chen, Ziqi Chen, Zijian Zhu, Yang Wen, Shaogang Qin, Yibing Bao, Ting Zhao, Hengxing Li, Longping Liu, Ming Hao, Jianbin Wang, Fengchao Wang, Haibin Wang, Bo Zhou, Hua Zhang, Guoliang Xia, Chao Wang
Rationale: Premature ovarian insufficiency (POI) is an accelerated reduction in ovarian function inducing infertility. Folliculogenesis defects have been reported to trigger POI as a consequence of ovulation failure. However, the underlying mechanisms remain unclear due to the genetic complexity and heterogeneity of POI./nMethods: We used whole genome sequencing (WGS), conditional knockout mouse models
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Tripeptidyl peptidase II coordinates the homeostasis of calcium and lipids in the central nervous system and its depletion causes presenile dementia in female mice through calcium/lipid dyshomeostasis-induced autophagic degradation of CYP19A1 Theranostics (IF 12.4) Pub Date : 2024-1-27 Jin Zhao, Chengtong He, Xueyu Fan, Lin Wang, Liao Zhao, Hui Liu, Wujun Shen, Sanwei Jiang, Kaixuan Pei, Jingjing Gao, Yawei Qi, Yang Liu, Junqiang Zhao, Ruiling Zhang, Chengbiao Lu, Jia Tong, Jisen Huai
Rationale: Tripeptidyl peptidase II (TPP2) has been proven to be related to human immune and neurological diseases. It is generally considered as a cytosolic protein which forms the largest known protease complex in eukaryotic cells to operate mostly downstream of proteasomes for degradation of longer peptides. However, this canonical function of TPP2 cannot explain its role in a wide variety of biological
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Enhancing anti-tumor potential: low-intensity vibration suppresses osteosarcoma progression and augments MSCs' tumor-suppressive abilities Theranostics (IF 12.4) Pub Date : 2024-1-27 Xue Xiong, Qingji Huo, Kexin Li, Changpeng Cui, Chunyi Chang, Charles Park, BonHeon Ku, Chin-Suk Hong, HeeChang Lim, Pankita H. Pandya, M. Reza Saadatzadeh, Khadijeh Bijangi-Vishehsaraei, Chien-Chi Lin, Melissa A. Kacena, Karen E. Pollok, Andy Chen, Jing Liu, William R. Thompson, Xue-Lian Li, Bai-Yan Li, Hiroki Yokota
Rationale: Osteosarcoma (OS), a common malignant bone tumor, calls for the investigation of novel treatment strategies. Low-intensity vibration (LIV) presents itself as a promising option, given its potential to enhance bone health and decrease cancer susceptibility. This research delves into the effects of LIV on OS cells and mesenchymal stem cells (MSCs), with a primary focus on generating induced
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Re-analysis of single-cell transcriptomics reveals a critical role of macrophage-like smooth muscle cells in advanced atherosclerotic plaque Theranostics (IF 12.4) Pub Date : 2024-1-27 Xue Gong, Yunchang Liu, Huiying Liu, Nian Cao, Liping Zeng, Miao Tian, Chunyu Zeng, Yijie Hu, Runjun Zhang, Yundai Chen, Gengze Wu
Aims: Smooth muscle cell (SMC) remodeling poses a critical feature in the development and progression of atherosclerosis. Although fate mapping and in silicon approaches have expanded SMC phenotypes in atherosclerosis, it still remains elusive about the contributions of individual SMC phenotypes and molecular dynamics to advanced atherosclerotic plaque./nMethods: Using single-cell transcriptome, we
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Transfer RNA derived fragment, tRF-Glu-CTC, aggravates the development of neovascular age-related macular degeneration Theranostics (IF 12.4) Pub Date : 2024-1-27 Yu Liang, Lingjie Kong, Yuelu Zhang, Yihan Zhang, Mingsu Shi, Jiaqiu Huang, Hongyu Kong, Siyi Qi, Yunlong Yang, Jiaxu Hong, Meidong Zhu, Xiangjia Zhu, Xinghuai Sun, Shujie Zhang, Lianqun Wu, Chen Zhao
Rationale: Angiogenesis expedites tissue impairment in many diseases, including age-related macular degeneration (AMD), a leading cause of irreversible blindness in elderly. A substantial proportion of neovascular AMD patients, characterized by aberrant choroidal neovascularization (CNV), exhibit poor responses or adverse reactions to anti-VEGF therapy. Herein, we aimed to unveil the function of newly
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The mitochondria‒paraspeckle axis regulates the survival of transplanted stem cells under oxidative stress conditions Theranostics (IF 12.4) Pub Date : 2024-1-27 Meng Zhao, Shuyun Liu, Yizhuo Wang, Ke Lv, Peng Lou, Pingya Zhou, Jiaying Zhu, Lan Li, Jingqiu Cheng, Yanrong Lu, Jingping Liu
Rationale: Stem cell-based therapies have emerged as promising tools for tissue engineering and regenerative medicine, but their therapeutic efficacy is largely limited by the oxidative stress-induced loss of transplanted cells at injured tissue sites. To address this issue, we aimed to explore the underlying mechanism and protective strategy of ROS-induced MSC loss./nMethods: Changes in TFAM (mitochondrial
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Degraders in epigenetic therapy: PROTACs and beyond Theranostics (IF 12.4) Pub Date : 2024-1-27 Xing-Jie Dai, Shi-Kun Ji, Meng-Jie Fu, Gao-Zhi Liu, Hui-Min Liu, Shao-Peng Wang, Liang Shen, Ning Wang, Piet Herdewijn, Yi-Chao Zheng, Sai-Qi Wang, Xiao-Bing Chen
Epigenetics refers to the reversible process through which changes in gene expression occur without changing the nucleotide sequence of DNA. The process is currently gaining prominence as a pivotal objective in the treatment of cancers and other ailments. Numerous drugs that target epigenetic mechanisms have obtained approval from the Food and Drug Administration (FDA) for the therapeutic intervention
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Metal nanoparticle hybrid hydrogels: the state-of-the-art of combining hard and soft materials to promote wound healing Theranostics (IF 12.4) Pub Date : 2024-1-27 Yuxiang Wang, Mengya Zhang, Zhenzhen Yan, Shizhao Ji, Shichu Xiao, Jie Gao
Wounds represent a grave affliction that profoundly impacts human well-being. Establishing barriers, preventing infections, and providing a conducive microenvironment constitute the crux of wound therapy. Hydrogel, a polymer with an intricate three-dimensional lattice, serves as a potent tool in erecting physical barriers and nurturing an environment conducive to wound healing. This enables effective
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Therapeutic effects of a novel electrode for transcranial direct current stimulation in ischemic stroke mice Theranostics (IF 12.4) Pub Date : 2024-1-21 Da Hee Jung, Jae Ho Lee, Hong Ju Lee, Jang Woo Park, Young-Jin Jung, Hwa Kyoung Shin, Byung Tae Choi
Rationale: Non-invasive transcranial direct current stimulation (tDCS), a promising stimulation tool to modulate a wide range of brain disorders, has major limitations, such as poor cortical stimulation intensity and focality. We designed a novel electrode for tDCS by conjugating a needle to a conventional ring-based high-definition (HD) electrode to enhance cortical stimulation efficacy./nMethod:
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Efficient PD-L1 imaging of murine glioblastoma with FUS-aided immunoPET by leveraging FcRn-antibody interaction. Theranostics (IF 12.4) Pub Date : 2023-10-16 Céline Chevaleyre,Anthony Novell,Nicolas Tournier,Ambre Dauba,Steven Dubois,Dimitri Kereselidze,Erwan Selingue,Benoit Jego,Bernard Maillère,Benoit Larrat,Hervé Nozach,Charles Truillet
Rationale: The passage of antibodies through the blood-brain barrier (BBB) and the blood-tumoral barrier (BTB) is determinant not only to increase the immune checkpoint inhibitors efficacy but also to monitor prognostic and predictive biomarkers such as the programmed death ligand 1 (PD-L1) via immunoPET. Although the involvement of neonatal Fc receptor (FcRn) in antibody distribution has been demonstrated
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Tat-NTS peptide protects neurons against cerebral ischemia-reperfusion injury via ANXA1 SUMOylation in microglia. Theranostics (IF 12.4) Pub Date : 2023-10-16 Huijuan Zhou,Lulu Yan,Hezhou Huang,Xing Li,Qian Xia,Lu Zheng,Bin Shao,Qian Gao,Ning Sun,Jing Shi
Rationale: Recent studies indicate that microglial activation and the resulting inflammatory response could be potential targets of adjuvant therapy for ischemic stroke. Many studies have emphasized a well-established function of Annexin-A1 (ANXA1) in the immune system, including the regulation of microglial activation. Nevertheless, few therapeutic interventions targeting ANXA1 in microglia for ischemic
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Engineered oncolytic bacteria HCS1 exerts high immune stimulation and safety profiles for cancer therapy. Theranostics (IF 12.4) Pub Date : 2023-10-16 Yujie Sun,Yanxia Guo,Xiaoqing Liu,Jinling Liu,Honglai Sun,Zhongying Li,Min Wen,Sheng-Nan Jiang,Wenzhi Tan,Jin Hai Zheng
Background and rationale: Attenuated Salmonella typhimurium VNP20009 has been used to treat tumor-bearing mice and entered phase I clinical trials. However, its mild anticancer effect in clinical trials may be related to insufficient bacterial colonization and notable adverse effects with increasing dosages. Guanosine 5'-diphosphate-3'-diphosphate (ppGpp) synthesis-deficient Salmonella is an attenuated
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Nanobody-mediated SPECT/CT imaging reveals the spatiotemporal expression of programmed death-ligand 1 in response to a CD8+ T cell and iNKT cell activating mRNA vaccine. Theranostics (IF 12.4) Pub Date : 2023-10-09 Thomas Ertveldt,Sofie Meulewaeter,Yannick De Vlaeminck,Oscar Olarte,Katrijn Broos,Serge Van Calenbergh,Stephanie Bourgeois,Joke Deprez,Yves Heremans,Cleo Goyvaerts,Willem Staels,Stefaan De Smedt,Heleen Dewitte,Nick Devoogdt,Marleen Keyaerts,Rein Verbeke,Kurt Barbé,Ine Lentacker,Karine Breckpot
Rationale: Although promising responses are obtained in patients treated with immune checkpoint inhibitors targeting programmed death ligand 1 (PD-L1) and its receptor programmed death-1 (PD-1), only a fraction of patients benefits from this immunotherapy. Cancer vaccination may be an effective approach to improve the response to immune checkpoint inhibitors anti-PD-L1/PD-1 therapy. However, there
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Theranostics with photodynamic therapy for personalized medicine: to see and to treat. Theranostics (IF 12.4) Pub Date : 2023-10-09 Youchao Wang,Johannes Nikodemus Staudinger,Thomas L Mindt,Gilles Gasser
Photodynamic Therapy (PDT) is an approved treatment modality, which is presently receiving great attention due to its limited invasiveness, high selectivity and limited susceptibility to drug resistance. Another related research area currently expanding rapidly is the development of novel theranostic agents based on the combination of PDT with different imaging technologies, which allows for both therapy
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Engineering CAR-T cells for radiohapten capture in imaging and radioimmunotherapy applications. Theranostics (IF 12.4) Pub Date : 2023-10-08 Keifer Kurtz,Laura Eibler,Megan M Dacek,Lukas M Carter,Darren R Veach,Samantha Lovibond,Emma Reynaud,Sarah Qureshy,Michael R McDevitt,Christopher Bourne,Sebastien Monette,Blesida Punzalan,Shireen Khayat,Svena Verma,Adam L Kesner,Nai-Kong V Cheung,Heiko Schöder,Leah Gajecki,Sarah M Cheal,Steven M Larson,David A Scheinberg,Simone Krebs
Rationale: The in vivo dynamics of CAR-T cells remain incompletely understood. Novel methods are urgently needed to longitudinally monitor transferred cells non-invasively for biodistribution, functionality, proliferation, and persistence in vivo and for improving their cytotoxic potency in case of treatment failure. Methods: Here we engineered CD19 CAR-T cells ("Thor"-cells) to express a membrane-bound
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Piezo1 specific deletion in macrophage protects the progression of liver fibrosis in mice. Theranostics (IF 12.4) Pub Date : 2023-10-02 Shangfei Luo,Xiaoduo Zhao,Jintao Jiang,Bo Deng,Silin Liu,Honglin Xu,Qiaorui Tan,Yu'an Chen,Ziyan Zhang,Xianmei Pan,Rentao Wan,Xiaoting Chen,Youfen Yao,Jing Li
Background and Aims: Liver fibrosis is the common pathological pathway of chronic liver diseases and its mechanisms of which have not been fully declared. Macrophages play essential roles in progression of liver fibrosis partially by sensing abnormal mechanical signals. The aim of the study is to investigate the functions of macrophage Piezo1, a mechano-sensitive ion channel, in liver fibrosis. Approach
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Inhalation of acidic nanoparticles prevents doxorubicin cardiotoxicity through improvement of lysosomal function. Theranostics (IF 12.4) Pub Date : 2023-10-02 Yohan Santin,Karina Formoso,Fraha Haidar,Maria Del Pilar Oreja Fuentes,Florence Bourgailh,Nesrine Hifdi,Karim Hnia,Yosra Doghri,Jessica Resta,Camille Champigny,Séverine Lechevallier,Maximin Détrait,Grégoire Cousin,Malik Bisserier,Angelo Parini,Frank Lezoualc'h,Marc Verelst,Jeanne Mialet-Perez
Doxorubicin (Dox) is an effective anticancer molecule, but its clinical efficacy is limited by strong cardiotoxic side effects. Lysosomal dysfunction has recently been proposed as a new mechanism of Dox-induced cardiomyopathy. However, to date, there is a paucity of therapeutic approaches capable of restoring lysosomal acidification and function in the heart. Methods: We designed novel poly(lactic-co-glycolic
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Systemic delivery of glycosylated-PEG-masked oncolytic virus enhances targeting of antitumor immuno-virotherapy and modulates T and NK cell infiltration. Theranostics (IF 12.4) Pub Date : 2023-10-02 Yuzhi Liang,Bing Wang,Qingjing Chen,Xingyue Fu,Chenwei Jiang,Zhiwen Lin,Qiuyu Zhuang,Yongyi Zeng,Xiaolong Liu,Da Zhang
Rationale: Immuno-virotherapy has emerged as a promising approach for cancer treatment, as it directly and cytotoxically eliminates tumors with systemic immune stimulation. However, the clinical efficacy of this approach remains limited by inappropriate delivery routes, robust antiviral responses, and the tumor immunosuppressive microenvironment. Methods: To address these challenges, we propose a surface
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In vivo nanoparticle-based T cell imaging can predict therapy response towards adoptive T cell therapy in experimental glioma. Theranostics (IF 12.4) Pub Date : 2023-09-25 Jessica Hunger,Katharina Schregel,Berin Boztepe,Dennis Alexander Agardy,Verena Turco,Kianush Karimian-Jazi,Ina Weidenfeld,Yannik Streibel,Manuel Fischer,Volker Sturm,Rachel Santarella-Mellwig,Michael Kilian,Kristine Jähne,Katharina Sahm,Wolfgang Wick,Lukas Bunse,Sabine Heiland,Theresa Bunse,Martin Bendszus,Michael Platten,Michael O Breckwoldt
Rationale: Intrinsic brain tumors, such as gliomas are largely resistant to immunotherapies including immune checkpoint blockade. Adoptive cell therapies (ACT) including chimeric antigen receptor (CAR) or T cell receptor (TCR)-transgenic T cell therapy targeting glioma-associated antigens are an emerging field in glioma immunotherapy. However, imaging techniques for non-invasive monitoring of adoptively
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PET imaging of focused-ultrasound enhanced delivery of AAVs into the murine brain. Theranostics (IF 12.4) Pub Date : 2023-09-25 Javier Ajenjo,Jai Woong Seo,Josquin Foiret,Bo Wu,Marina Nura Raie,James Wang,Brett Zain Fite,Nisi Zhang,Rim Malek,Corinne Beinat,Noeen Malik,David Alexander Anders,Katherine W Ferrara
Rationale: Despite recent advances in the use of adeno-associated viruses (AAVs) as potential vehicles for genetic intervention of central and peripheral nervous system-associated disorders, gene therapy for the treatment of neuropathology in adults has not been approved to date. The currently FDA-approved AAV-vector based gene therapies rely on naturally occurring serotypes, such as AAV2 or AAV9,
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1α,25(OH)2D3 ameliorates insulin resistance by alleviating γδ T cell inflammation via enhancing fructose-1,6-bisphosphatase 1 expression. Theranostics (IF 12.4) Pub Date : 2023-09-25 Peng Li,Ke Li,Wenhui Yuan,Yuqi Xu,Ping Li,Ruan Wu,Jingru Han,Zhinan Yin,Ligong Lu,Yunfei Gao
Background: Chronic inflammation caused by immune cells is the central link between obesity and insulin resistance. Targeting the inflammatory process is a highly promising method for reversing systemic insulin resistance. Methods: Blood samples were prospectively collected from 68 patients with type 2 diabetes. C57BL/6J mice were fed either a high-fat diet (HFD) or normal chow (NC). We performed phenotypical