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Mapping the transporter-substrate interactions of the Trypanosoma cruzi NB1 nucleobase transporter reveals the basis for its high affinity and selectivity for hypoxanthine and guanine and lack of nucleoside uptake Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2024-02-23 Mustafa M. Aldfer, Fabian Hulpia, Serge van Calenbergh, Harry P. De Koning
is a protozoan parasite and the etiological agent of Chagas disease, a debilitating and sometimes fatal disease that continues to spread to new areas. Yet, Chagas disease is still only treated with two related nitro compounds that are insufficiently effective and cause severe side effects. Nucleotide metabolism is one of the known vulnerabilities of , as they are auxotrophic for purines, and nucleoside
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Mechanisms of adaptation and evolution in Toxoplasma gondii Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2024-02-13 Sergio O. Angel, Laura Vanagas, Andres M. Alonso
has high host flexibility, infecting all nucleated cells of mammals and birds. This implies that during its infective process the parasite must constantly adapt to different environmental situations, which in turn leads to modifications in its metabolism, regulation of gene transcription, translation of mRNAs and stage specific factors. There are conserved pathways that support these adaptations, which
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A systematic review and meta-analysis on the current status of anthelmintic resistance in equine nematodes: A global perspective Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-11-28 Enjia Cai, Rongzheng Wu, Yuhong Wu, Yu Gao, Yiping Zhu, Jing Li
Background The intensive application of anthelmintics in equine has led to considerable resistance in cyathostomins and Parascaris equorum. It has been well documented that benzimidazole (BZ) and pyrantel resistance is widespread in cyathostomins and Parascaris equorum. Since no new classes of anthelmintic have been introduced in the last 40 years, it is critical to be aware of the current risk factors
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Alterations in the metabolism of Pseudosuccinea columella (Mollusca: Gastropoda) caused by Heterorhabditis bacteriophora HP88 (Rhabditida: Heterorhabditidae) Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-11-23 Victor Menezes Tunholi, Natânia do Carmo Sperandio, Vinícius Menezes Tunholi-Alves, Lorena Souza Castro Altoé, Melissa Carvalho Machado do Couto-Chambarelli, Ludimila Santos Amaral, Caio Márcio de Oliveira Monteiro, Isabella Vilhena Freire Martins
The gastropod Pseudosuccinea columella participates in the dissemination of Fasciola hepatica in the environment, acting as the main intermediate host of this parasite in Brazil. The present study sought to elucidate the possible pathogenic effects of the entomopathogenic nematode (EPN) Heterorhabditis bacteriophora on P. columella, by evaluating the influence of infection on alanine aminotransferase
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Imaging of genomic loci in Trypanosoma brucei using an optimised LacO-LacI system Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-11-03 James Budzak, Ione Goodwin, Calvin Tiengwe, Gloria Rudenko
Visualisation of genomic loci by microscopy is essential for understanding nuclear organisation, particularly at the single cell level. One powerful technique for studying the positioning of genomic loci is through the Lac Operator-Lac Repressor (LacO-LacI) system, in which LacO repeats introduced into a specific genomic locus can be visualised through expression of a LacI-protein fused to a fluorescent
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Elucidating the structure-function attributes of a trypanosomal arginyl-tRNA synthetase Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-10-16 Pratyasha Bhowal, Bappaditya Roy, Sayak Ganguli, Gabor L. Igloi, Rajat Banerjee
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Eimeria granulosa causes spots visible through the serous membrane of small intestine in sheep Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-09-18 Yuanyuan Chen, Jing Liu, Xiaolei Liu, Qiaocheng Chang, Xiaoxiao Ma, Qinwei Xu
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RNA editing catalytic complexes edit multiple mRNA sites non-processively in Trypanosoma brucei Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-09-22 Jason Carnes, Suzanne M. McDermott, Kenneth Stuart
RNA editing generates mature mitochondrial mRNAs in T. brucei by extensive uridine insertion and deletion at numerous editing sites (ESs) as specified by guide RNAs (gRNAs). The editing is performed by three RNA Editing Catalytic Complexes (RECCs) which each have a different endonuclease in addition to 12 proteins in common resulting in RECC1 that is specific for deletion ESs and RECC2 and RECC3 that
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Model nematodes as a practical innovation to promote high throughput screening of natural products for anthelmintics discovery in South Asia: Current challenges, proposed practical and conceptual solutions Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-09-18 Muntasir Kamal, Suprabhat Mukherjee, Bishnu Joshi, Zia-ud-Din Sindhu, Phurpa Wangchuk, Shawkat Haider, Nurnabi Ahmed, Md. Hasanuzzaman Talukder, Timothy G. Geary, Arun K. Yadav
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Role of autophagy in stress and drug-responsive cell death in Entamoeba histolytica and its cross-talk with apoptosis-inducing factor Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-09-12 Anwesha Bandyopadhyay, Sudip Kumar Ghosh
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Identification and validation of reference genes of circulating microRNAs for use as control in gestational toxoplasmosis Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-09-04 Ingrid de Siqueira Pereira, Allecineia Bispo da Cruz, Marta Marques Maia, Francieli Marinho Carneiro, Ricardo Gava, Lígia Cosentino Junqueira Franco Spegiorin, Cinara Cássia Brandão, Ingrid Gomes de Campos Truzzi, Geraldo Magela de Faria Junior, Luiz Carlos de Mattos, Vera Lucia Pereira-Chioccola, Cristina Silva Meira-Strejevitch
Toxoplasmosis causes serious harm to the fetus, as tachyzoite dissemination, during pregnancy in women developing the primo-infection. The microRNAs (miRNAs) are small non-coding RNAs, which have regulatory roles in cells by silencing messenger RNA. Circulating miRNA are promising biomarkers for diagnosis and prognosis of numerous diseases. The miRNAs levels are estimated by quantitative real-time
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Characterisation of TcFUT1, a mitochondrial fucosyltransferase from Trypanosoma cruzi Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-08-29 Jose Carlos Paredes Franco, Maria Lucia Sampaio Guther, Marta L. Lima, Michael A.J. Ferguson
Previous work has shown that the TbFUT1 and LmjFUT1 genes encode essential fucosyltransferases located inside the single mitochondria of the protozoan parasites Trypanosoma brucei and Leishmania major, respectively. However, nothing was known about the orthologous gene TcFUT1 or its gene product in Trypanosoma cruzi, aetiological agent of Chagas disease. In this study, we describe the overexpression
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Characterization of the major surface glycoconjugates of Trypanosoma theileri Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-08-29 Rupa Nagar, Isobel Hambleton, Michele Tinti, Mark Carrington, Michael A.J. Ferguson
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Does hydrogen peroxide contribute to the immunity against Malaria induced by whole attenuated plasmodial sporozoites? Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-08-19 Bruno Douradinha
Plasmodium sporozoites can block apoptotic pathways within host hepatocytes, ensuring the survival of the parasite. However, attenuated plasmodial sporozoites are unable to prevent apoptosis, which provides many parasite antigens to immune cells. This exposure leads to protection against Malaria in both human and animal models. If these hosts are later inoculated with infectious sporozoites, apoptosis
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Novel anti-Acanthamoebic properties of raloxifene sulfonate/sulfamate derivatives Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-08-09 Ruqaiyyah Siddiqui, Mohammed I. El-Gamal, Sreedevi Sajeev, Seyed-Omar Zaraei, Naveed Ahmed Khan
Acanthamoeba are known to cause a vision threatening eye infection typically due to contact lens wear, and an infection of the central nervous system. The ability of these amoebae to switch phenotypes, from an active trophozoite to a resistant cyst form is not well understood; the cyst stage is often resistant to chemotherapy, which is of concern given the rise of contact lens use and the ineffective
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Evaluation of PEN2-ATP6AP1 axis as an antiparasitic target for metformin based on phylogeny analysis and molecular docking Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-07-18
Background Metformin (Met), the first-line drug used in the treatment for type 2 diabetes mellitus, is effective against a variety of parasites. However, the molecular target of Met at clinical dose against various parasites remains unclear. Recently, low-dose Met (clinical dose) has been reported to directly bind PEN2 (presenilin enhancer protein 2) and initiate the lysosomal glucose-sensing pathway
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Amino acid usage and protein expression levels in the flatworm Schistosoma mansoni Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-07-19 Guillermo Lamolle, Andrés Iriarte, Diego Simón, Héctor Musto
Schistosoma mansoni is a parasitic flatworm that causes a human disease called schistosomiasis, or bilharzia. At the genomic level, S. mansoni is AT-rich, but has some compositional heterogeneity. Indeed, some regions of its genome are GC-rich, mainly in the regions located near the extreme ends of the chromosomes. Recently, we showed that, despite the strong bias towards A/T ending codons, highly
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Coenzyme Q10 exhibits anti-inflammatory and immune-modulatory thereby decelerating the occurrence of experimental cerebral malaria Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-06-27 James Nyabuga Nyariki, Njogu M. Kimani, Peter Shikuku Kibet, Geoffrey K. Kinuthia, Alfred Orina Isaac
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Anti-parasitic activity of a chimeric peptide Cecropin A (2−8)-Melittin (6−9) (CM11) against tachyzoites of Toxoplasma gondii and the BALB/c mouse model of acute toxoplasmosis Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-06-20 Zahra Abbasali, Majid Pirestani, Abdolhossin Dalimi, Milad Badri, Mahdi Fasihi-Ramandi
Toxoplasmosis is a zoonotic disease that infects most animals, including humans. Pyrimethamine/sulfadiazine is the standard treatment for toxoplasmosis. Although this treatment has been successful, it is often associated with side effects that cannot be tolerated. Therefore, various compounds have been proposed as alternative treatments for toxoplasmosis. Antimicrobial peptides (AMPs) act on various
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Therapeutic activity of eugenol towards mitigation of anaemia and oxidative organ damage caused by Plasmodium berghei Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-06-15 Mukhtar Adeiza Suleiman, Mohammed Aliyu Usman, Samson Olayinka Awogbamila, Umar Adam Idris, Fatima Binta Ibrahim, Halimat-Oyibo Mohammed
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Disruption of a DNA fragment that encodes the microneme adhesive repeat domain-containing region of the BBOV_III011730 does not affect the blood stage growth of Babesia bovis in vitro Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-06-12 Bumduuren Tuvshintulga, Azirwan Guswanto, Arifin Budiman Nugraha, Thillaiampalam Sivakumar, Rika Umemiya-Shirafuji, Naoaki Yokoyama
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Diclazuril-induced expression of CDK-related kinase 2 in the second-generation merozoites of Eimeria tenella Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-06-09 Bian-hua Zhou, Hai-yan Ding, Jing-yun Yang, Jun Chai, Hong-wei Guo, Er-jie Tian
Diclazuril is a classic anticoccidial drug. The key molecules of diclazuril in anticoccidial action allows target screening for the development of anticoccidial drugs. Cyclin-dependent kinases (CDK) are prominent target proteins in apicomplexan parasites. In this study, a diclazuril anticoccidiosis animal model was established, and the transcription and translation levels of the CDK-related kinase
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Transgenic overexpression of heat shock protein (HSP83) enhances protein kinase A activity, disrupts GP63 surface protease expression and alters promastigote morphology in Leishmania amazonensis Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-05-05 Catherine S. Nation, Isabel Stephany-Brassesco, Ben L. Kelly, Juan C. Pizarro
Leishmania parasites undergo morphological changes during their infectious life cycle, including developmental transitions within the sandfly vector, culminating in metacyclic stages that are pre-adapted for infection. Upon entering vertebrate host phagocytes, Leishmania differentiate into intracellular amastigotes, the form that is ultimately transmitted back to the vector to complete the life cycle
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Current status and progress in the omics of Clonorchis sinensis Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-04-29 Nian Wang, Zhuanling Zhang, Lisi Huang, Tingjin Chen, Xinbing Yu, Yan Huang
Clonorchis sinensis (C. sinensis) is a fish-borne trematode that inhabits the bile duct of mammals including humans, cats, dogs, rats, and so on. In the complex life cycle of C. sinensis, the worm develops successively in two intermediate hosts in fresh water and one definitive host. What’s more, it undergoes eight developmental stages with a distinct morphology. Clonorchiasis, caused by C. sinensis
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Characteristics of Th9 cells in Schistosoma japonicum-infected C57BL/6 mouse mesenteric lymph node Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-04-20 Huaina Qiu, Ruohan Wang, Junmin Xing, Lu Li, Zhiyan Gao, Jiajie Li, Chao Fang, Feihu Shi, Feng Mo, Lin Liu, Yi Zhao, Hongyan Xie, Shan Zhao, Jun Huang
Interleukin 9 (IL-9) is an effective cytokine secreted by newly defined Th9 cells, which is involved in allergic and infectious diseases. In this study, lymphocytes were isolated from mesenteric lymph node (MLN), spleen, liver, lung, and Peyer’s patches (PP) of C57BL/6 mice 5–6 weeks after S. japonicum infection, intracellular cytokine staining was done to detect the percentage of IL-9-producing CD4+
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In vitro and in vivo evaluation of efficacy of berberine chloride: Phyto-alternative approach against Trypanosoma evansi infection Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-04-20 Snehil Gupta, Sukhdeep Vohra, Khushboo Sethi, Ruma Rani, Surbhi Gupta, Sanjay Kumar, Rajender Kumar
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Characterization of 21 microsatellite loci for the precocious, grass-shrimp trematode Alloglossidium renale Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-04-20 Jenna M. Hulke, Charles D. Criscione
We developed microsatellite markers to use in studying the population genetics of the trematode Alloglossidium renale, a fluke with a precocious life cycle where sexual maturation occurs in a grass shrimp. Among 21 tested loci in a Mississippi population sample, 14 were polymorphic, 12 of which significantly deviated from Hardy-Weinberg Equilibrium (HWE). We estimated identity disequilibrium (ID) to
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Designing a multi-epitope chimeric protein from different potential targets: A potential vaccine candidate against Plasmodium Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-04-20 Sanasam Bijara Devi, Sanjeev Kumar
Malaria is an infectious disease that has been a continuous threat to mankind since the time immemorial. Owing to the complex multi-staged life cycle of the plasmodium parasite, an effective malaria vaccine which is fully protective against the parasite infection is urgently needed to deal with the challenges. In the present study, essential parasite proteins were identified and a chimeric protein
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Identification of Omega-class glutathione transferases in helminths of the Taeniidae family Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-04-05 Sebastián Miles, Gustavo Mourglia-Ettlin, Verónica Fernández
Glutathione transferase enzymes (GSTs) are believed to be a major detoxification system in helminth parasites and have been associated with immunomodulation of the host response. Echinococcus granulosus sensu lato (s.l.) is a cestode parasite known to express at least five different GSTs, but no Omega-class enzymes have been reported in this parasite or in any other cestode. Herein we report the identification
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Genetic diversity and natural selection of rif gene (PF3D7_1254800) in the Plasmodium falciparum global populations Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-03-12 Shao-Jie Xu, Hai-Mo Shen, Yan-Bing Cui, Shen-Bo Chen, Bin Xu, Jun-Hu Chen
To reveal the genetic characteristics of one member of the Plasmodium falciparum repetitive interspersed family (rif), we sequenced the rif gene (PF3D7_1254800) in 53 field isolates collected from Ghana-imported cases into China and compared them with 350 publicly available P. falciparum rif sequences from global populations. In the Ghana-imported population, the nucleotide diversities were 0.05714
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A simple alkali lysis method for Plasmodium falciparum DNA extraction from filter paper blood samples Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-02-21 Zheng Xiang, Danlin Li, Siqi Wang, Ting Shen, Wen He, Mier Li, Weilin Zeng, Xi Chen, Yanrui Wu, Liwang Cui, Zhaoqing Yang
A fast, simple, easy, efficient, and inexpensive method for DNA extraction from malaria parasites collected on filter paper would be very useful for molecular surveillance. The quality and quantity of DNA are critical to molecular diagnosis and analysis. Here, we developed a simple alkali lysis method for DNA extraction from blood samples on filter paper. The results showed that 10–50 mM NaOH and deionized
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Analysis of sheep peripheral blood mononuclear cells in response to Echinococcus granulosus microRNA-71 overexpression Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-02-02 Yating Li, Lujun Yan, Duojie Ci, Rui Li, Wanjing Li, Tianqi Xia, Hengzhi Shi, Mazhar Ayaz, Yadong Zheng, Pu Wang
Cyst echinococcosis, caused by Echinococcus granulosus, remains a zoonotic disease posing a great threat to public health and meat production industry. Sheep infected with E. granulosus show relatively high abundance of egr-miR-71 in the sera, but its role is unknown. Using bioinformatics and cell migration and Transwell assays, we comparatively analyzed the proteomes and cell invasion of sheep PBMCs
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Dynamics of parasite growth in genetically diverse Plasmodium falciparum isolates Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-01-31 Standwell C. Nkhoma, Amel O.A. Ahmed, Danielle Porier, Sujatha Rashid, Rebecca Bradford, Robert E. Molestina, Timothy T. Stedman
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In silico analysis of two Haemonchus spp. serine protease peptides (S28) and their immunomodulatory activity in vitro Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-01-18 René Camas-Pereyra, Génesis A. Bautista-García, Guillermina Avila, Yazmin Alcala-Canto, Jocelyn Maza-Lopez, David E. Reyes-Guerrero, Rosa Isabel Higuera-Piedrahita, María Eugenia López-Arellano
The aim of this study was to evaluate the in vitro immune modulation of two de novo peptides with hypothetical identity to the serine protease family (S28) from Haemonchus spp. Expression of mRNAs encoding these peptides was confirmed by RTqPCR in L3 and adult stage parasites. Antibodies from serum samples collected from an H. contortus-infected lamb at 60 days post infection detected both peptides
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Heat shock protein 60 in parasitic helminths: A role in immune responses and therapeutic applications Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-01-11 Xuedong He, Yue Sun, Fang Yang, Guanghui Zheng, Rui Li, Mengqi Liu, Wanjing Li, Dong-Hui Zhou, Yadong Zheng
Heat shock protein 60 (HSP60) is an unique member of the heat shock protein family, being involved in parasite infections. To cope with harsh environments where parasites live, HSP60s are indispensable and involved in a variety of biological processes. HSP60s have relative low similarity among parasites, but their ATPase /Mg2+ active sites are highly conserved. The interactions of HSP60s with signaling
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The microbiota, the malarial parasite, and the mosquito [MMM] – A three-sided relationship Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-01-13 Sathishkumar Vinayagam, Devianjana Rajendran, Kathirvel Sekar, Kaviyarasi Renu, Kamaraj Sattu
The mosquito gut microbiota is vital to the proper functioning of the host organism. Mosquitoes may benefit from this microbiota in their guts because it promotes factors including blood digestion, fecundity, metamorphosis, and living habitat and inhibits malarial parasites (Plasmodium) growth or transmission. In this overview, we analyzed how mosquitoes acquire their gut microbiota, characterized
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Modular nanotheranostic agents for protistan parasitic diseases: Magic bullets with tracers Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2023-01-02 Sutherland Kester Maciver, Sumayah Abdelnasir, Ayaz Anwar, Ruqaiyyah Siddiqui, Naveed Ahmed Khan
Protistan parasitic infections contribute significantly to morbidity and mortality, causing more than 2 billion human infections annually. However, current treatments are often limited; due to ineffective drugs and drug resistance, thus better options are urgently required. In the present context, theranostics agents are those that offer simultaneous detection, diagnosis and even treatment of protistan
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Phylogeny and population structure of Echinococcus granulosus (sensu stricto) based on full-length cytb-nad2-atp6 mitochondrial genes – First report from Sialkot District of Pakistan Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-12-27 Mughees Aizaz Alvi, Rana Muhammad Athar Ali, Li Li, Muhammad Saqib, Warda Qamar, Ali Hassan, Muzafar Ghafoor, Siddiq Ur Rahman, Muhammad Umar Zafar Khan, Bao-Quan Fu, Youyu Liu, Hong Yin, Hong-Bin Yan, Wan-Zhong Jia
Cystic echinococcosis is a zoonotic disease of livestock having serious economic setbacks. The etiological agents of the disease belong to Echinococcus granulosus sensu lato. Despite of worldwide distribution of the disease, the molecular studies mainly employ amplification of cox1, nad1 and nad5 genes. To further strengthen the knowledge about significance of other molecular markers and to investigate
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Characteristics of γδTCR on myeloid cells from C57BL/6 mice with Plasmodium yoelii nigeriensis infection Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-12-09 Dianhui Chen, Feng Mo, Meiling Liu, Yongjing Ma, Lin Liu, Junmin Xing, Feihu Shi, Anqi Xie, Hongyan Xie, Xingfei Pan, Xinhua Wang, Jun Huang
Recently, there is a paucity of studies focus on the characteristics of myeloid cells which expressed γδTCR. The aim of this study was to observe the properties of γδTCR-expressing myeloid cells in the spleen of C57BL/6 mice infected by P. yoelii nigeriensis NSM. Haematoxylin-eosin (HE) staining was used to observe pathological changes in the spleens from infected mice. The differentially expressed
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Deficiency in TLR4 impairs regulatory B cells production induced by Schistosome soluble egg antigen Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-11-28 Fang Tian, Kangwen Xian, Bin Yang, Qiufang Duan, Li Qian, Chanhong Shi
Regulatory B cells (Bregs) producing IL-10 have negative regulatory function. Several studies have shown the important roles for Toll-like receptor 2 (TLR2), TLR4, and TLR9 ligation in the development of Bregs. We have reported that Schistosome soluble egg antigen (SEA) induced the production of Bregs. However, it remains unclear whether such activation is via the TLR pathway. The present study showed
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Praziquantel: An update on the mechanism of its action against schistosomiasis and new therapeutic perspectives Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-11-11 Ranielly Araujo Nogueira, Maria Gabriela Sampaio Lira, Irlla Correia Lima Licá, Gleycka Cristine Carvalho Gomes Frazão, Vitor Augusto Ferreira dos Santos, Antonio Camilo Correia Mendes Filho, João Gustavo Mendes Rodrigues, Guilherme Silva Miranda, Rafael Cardoso Carvalho, Flávia Raquel Fernandes Nascimento
Praziquantel (PZQ) is the drug of choice for the treatment of all forms of schistosomiasis, although its mechanisms of action are not completely understood. PZQ acts largely on adult worms. This narrative literature review describes what is known about the mechanisms of action of PZQ against schistosomes from in vitro and in vivo studies and highlights the molecular targets in parasites and immune
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Structural characterization of glutamyl-tRNA synthetase (GluRS) from Plasmodium falciparum Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-11-09 Vivek Kumar Sharma, Jyoti Chhibber-Goel, Manickam Yogavel, Amit Sharma
Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes in protein translation machinery that provide the charged tRNAs needed for protein synthesis. Over the past decades, aaRSs have been studied as anti-parasitic, anti-bacterial, and anti-fungal drug targets. This study focused on the cytoplasmic glutamyl-tRNA synthetase (GluRS) from Plasmodium falciparum, which belongs to class Ib in aaRSs. GluRS
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Application of a low-cost, specific, and sensitive loop-mediated isothermal amplification (LAMP) assay to detect Plasmodium falciparum imported from Africa Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-10-29 Jiaqi Zhang, Xi Chen, Maohua Pan, Yucheng Qin, Hui Zhao, Qi Yang, Xinxin Li, Weilin Zeng, Zheng Xiang, Yanrui Wu, Mengxi Duan, Xiaosong Li, Xun Wang, Dominique Mazier, Yanmei Zhang, Wenya Zhu, Kemin Sun, Yiman Wu, Zhaoqing Yang
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Descriptive and functional analyses of four cyclin proteins in Trichomonas vaginalis Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-10-20 Karla López-Pacheco, Roberto Hernández, Imelda López-Villaseñor
Trichomonas vaginalis is an early divergent protozoan parasite that causes trichomoniasis, the most common non-viral sexually transmitted infection. In metazoans, there is abundant and detailed research on the cell cycle and the components involved in the regulation mechanisms. Regulators such as the cyclin-dependent kinases (CDKs) and cyclins activate the highly regulated processes of cell division
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Construction of constitutive expression of Eimeria tenella eukaryotic initiation factor U6L5H2 on the surface of Lactobacillus plantarum and evaluation of its immunoprotective efficiency against chicken coccidiosis Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-10-19 Lingyu Sun, Yaru Lu, Ningning Zhao, Yakun Wang, Bingxiang Wang, Huihui Li, Zhiyuan Wu, Hongmei Li, Xiao Zhang, Xiaomin Zhao
Lactobacillus strains exhibit preferable properties that make them attractive candidates for vaccine delivery systems because of their ability to regulate intestinal mucosal immunity in the body. To date, live Lactobacillus delivery vaccines reported for the defense against Eimeria tenella have been inducer-dependent systems whose applications are significantly limited due to their unattainable induction
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The Strongyloides bioassay toolbox: A unique opportunity to accelerate functional biology for nematode parasites Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-10-12 Luke C. Cadd, Bethany Crooks, Nikki J. Marks, Aaron G. Maule, Angela Mousley, Louise E. Atkinson
Caenorhabditis elegans is a uniquely powerful tool to aid understanding of fundamental nematode biology. While C. elegans boasts an unrivalled array of functional genomics tools and phenotype bioassays the inherent differences between free-living and parasitic nematodes underscores the need to develop these approaches in tractable parasite models. Advances in functional genomics approaches, including
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Biophysical description of Bromosulfophthalein interaction with the 28-kDa glutathione transferase from Schistosoma japonicum Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-10-03 Kagiso Pooe, Monare Thulo, Hattie Makumbe, Blessing Akumadu, Oluwatobin Otun, Chinyere Aloke, Ikechukwu Achilonu
Glutathione transferases (GSTs) are major detoxification enzymes vital for the survival and reproduction of schistosomes during infection in humans. Schistosoma encode two GST isoenzymes, the 26- and 28-kDa isoforms, that show different substrate specificities and cellular localisations. Bromosulfophthalein (BSP) has been identified and characterised as a potent 26-kDa Schistosoma japonicum GST (Sj26GST)
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MicroRNA-181b promotes schistosomiasis-induced hepatic fibrosis by targeting Smad7 Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-10-03 Shu Wang, Jianqiang Zhang, Hui Chen, Xiang Zhan, Hao Nie, Chao Wang, Yanxiang Zhang, Bing Zheng, Quan Gong
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Profiling of metabolic alterations in mice infected with malaria parasites via high-resolution metabolomics Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-10-06 Jyoti Chhibber-Goel, Anurag Shukla, Dhanasekaran Shanmugam, Amit Sharma
Background Malaria infection can result in distinct clinical outcomes from asymptomatic to severe. The association between patho-physiological changes and molecular changes in the host, and their correlation with severity of malaria progression is not fully understood. Methods In this study, we addressed mass spectrometry-based temporal profiling of serum metabolite levels from mice infected with Plasmodium
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Editorial - Strongyloides research in the post-genomics era Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-09-22 Louise E. Atkinson, Elissa A. Hallem
Abstract not available
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Synthesis, in vitro schistosomicidal activity and ultrastructural alterations caused by thiosemicarbazones and thiazolidinones against juvenile and adult Schistosoma mansoni worms (Sambon, 1907) Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-09-16 Rubens Emanoel Tavares da Rocha, Antônio Sérgio Alves de Almeira Júnior, Nairomberg Cavalcanti Portela Júnior, Amanda Vasconcelos do Nascimento, Nayara Maria Siqueira Leite, Jamerson Ferreira de Oliveira, Maria do Carmo Alves de Lima, Ana Paula Sampaio Feitosa, Maria Eliane Bezerra de Mélo, Fábio André Brayner, Luiz Carlos Alves
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Characterization of adjacent charged residues near the agonist binding site of the nematode UNC-49 GABA receptor Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-09-12 Everett Cochrane, Joshua Foster, Mohammad Hassan Khatami, Hendrick W. de Haan, Sean G. Forrester
The UNC-49 receptor is a Cys-loop GABA receptor that is unique to the nematode phylum. The receptor differs from mammalian GABA receptors both in amino acid sequence and pharmacology which highlights its potential as a novel anthelmintic target. Sequence differences within and near the various ligand-binding loops of the nematode receptor suggest that there could be structural differences compared
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Alpha-terthienyl increases filamentous actin of Entamoeba histolytica Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-09-06 Mayra Herrera-Martínez, Verónica Ivonne Hernández-Ramírez, Sarita Montaño, Bibiana Chávez-Munguía, Beatriz Hernández-Carlos, Patricia Talamás-Rohana
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Aminoacyl-tRNA synthetase (AARS) as an attractive drug target in neglected tropical trypanosomatid diseases-Leishmaniasis, Human African Trypanosomiasis and Chagas disease Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-08-18 Vikas Kushwaha, Neena Capalash
TriTryp diseases (Leishmaniasis, Human African Trypanosomiasis (HAT), and Chagas disease) are devastating parasitic neglected tropical diseases (NTDs) that affect billions of people in developing countries, cause high mortality in humans, and impose a large socio-economic burden. The current treatment options against tritryp diseases are suboptimal and challenging due to the emergence of resistance
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Is Strongyloides stercoralis hyperinfection induced by glucocorticoids a result of both suppressed host immunity and altered parasite genetics? Mol. Biochem. Parasitol. (IF 1.5) Pub Date : 2022-08-22 De'Broski R. Herbert, Jonathan D.C. Stoltzfus, Heather L. Rossi, David Abraham
The gastrointestinal (GI) nematode Strongyloides stercoralis (S.s.) causes human strongyloidiasis, a potentially life-threatening disease that currently affects over 600 million people globally. The uniquely pernicious aspect of S.s. infection, as compared to all other GI nematodes, is its autoinfective larval stage (L3a) that maintains a low-grade chronic infection, allowing undetectable persistence