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Low cycle number multiplex PCR: A novel strategy for the construction of amplicon libraries for next‐generation sequencing Electrophoresis (IF 2.9) Pub Date : 2024-03-27 Meng Lu, Xiuxiu Sun, Yuxin Zhao, Linlin Zheng, Junjie Lin, Chen Tang, Kaiyue Chao, Ye Chen, Kai Li, Yuxun Zhou, Junhua Xiao
Multiplex PCR is a critical step when preparing amplicon library for next‐generation sequencing. However, there are several challenges related to multiplex PCR including poor uniformity, nonspecific amplification, and primer–dimers. To address these issues, we propose a novel solution strategy that involves using a low cycle number (<10 cycles) in multiplex PCR and then employing carrier DNAs and magnetic
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Self‐propulsion of a droplet induced by combined diffusiophoresis and Marangoni effects Electrophoresis (IF 2.9) Pub Date : 2024-03-26 Yuhang Wang, Longtao Zheng, Gaojin Li
Chemically active droplets display complex self‐propulsion behavior in homogeneous surfactant solutions, often influenced by the interplay between diffusiophoresis and Marangoni effects. Previous studies have primarily considered these effects separately or assumed axisymmetric motion. To understand the full hydrodynamics, we investigate the motion of a two‐dimensional active droplet under their combined
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Lycium barbarum polysaccharide mitigated methamphetamine addiction and altered methamphetamine‐induced gut microbiota dysbiosis Electrophoresis (IF 2.9) Pub Date : 2024-03-26 Jingshen Zhuang, Qianling Chen, Luyao Xu, Dongfang Qiao, Xuebing Chen
Methamphetamine (MA) is a highly addictive mental stimulant, and MA abuse remains a significant public health problem worldwide, while effective treatment options are limited. Lycium barbarum polysaccharide (LBP), a major effective component extracted from Lycium barbarum, has potential health‐promoting effects on the nervous system; however, its role in MA dependence remains unclear. In this study
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Dielectric properties of human macrophages are altered by Mycobacterium tuberculosis infection Electrophoresis (IF 2.9) Pub Date : 2024-03-22 Matthew P. Johnson, Nira Lauterkorn, Rebecca Lewis, Fatima H. Labeed, Suzanne M. Hingley‐Wilson, Michael Pycraft Hughes
The analysis of cell electrophysiology for pathogenic samples at BSL3 can be problematic. It is virtually impossible to isolate infected from uninfected without a label, for example green fluorescent protein, which can potentially alter the cell electrical properties. Furthermore, the measurement of highly pathogenic organisms often requires equipment dedicated only for use with these organisms due
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Effects of Tween 20 addition on electrokinetic transport in a polydimethylsiloxane microchannel Electrophoresis (IF 2.9) Pub Date : 2024-03-21 Seyed Mojtaba Tabarhoseini, Joseph Bentor, Walter Johnson, Tzuen‐Rong Tzeng, Xiangchun Xuan
Tween 20 is frequently added to particle suspensions for reducing the particle–wall adhesion and particle–particle aggregation in microfluidic devices. However, the influences of Tween 20 on the fluid and particle behaviors have been largely ignored. We present in this work the first experimental study of the effects of Tween 20 addition on the electrokinetic transport of fluids and particles in a
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Numerical modeling and experimental optimization of Taylor dispersion analysis with and without an electric field Electrophoresis (IF 2.9) Pub Date : 2024-03-20 Yuri Chenyakin, David Da Yong Chen
Numerical modeling of Taylor dispersion analysis (TDA) was performed using COMSOL Multiphysics to facilitate better and faster optimization of the experimental conditions. Parameters, such as pressure, electric field, diameter, and length of capillary on the TDA conditions, were examined for particles with hydrodynamic radius (Rh) of 2.5–250 Å. The simulations were conducted using 25, 50, and 100 cm
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Back Cover: Improving DNA mixtures analysis using compound markers composed of InDels and SNPs screened from the whole genome with next‐generation sequencing Electrophoresis (IF 2.9) Pub Date : 2024-03-19 Mengyu Tan, Jiaming Xue, Qiushuo Wu, Yazi Zheng, Guihong Liu, Ranran Zhang, Mengna Wu, Jinlong Song, Yuanyuan Xiao, Dezhi Chen, Meili Lv, Miao Liao, Shengqiu Qu, Weibo Liang
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Front Cover: An embedded obstacle type micromixer‐concentration gradient generator based on capillary driven Electrophoresis (IF 2.9) Pub Date : 2024-03-19 Sisi Huang, Yihao Wu, Yifan Wang, Xiaoling Hu, Kui Song
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Developmental validation of a custom‐designed Multi‐InDel panel: A five‐dye multiplex amplification system for challenging DNA samples Electrophoresis (IF 2.9) Pub Date : 2024-03-19
This article corrects the following: First published: 30 November 2023 | https://doi.org/10.1002/elps.202300192 In the article Developmental validation of a custom-designed Multi-InDel panel: A five-dye multiplex amplification system for challenging DNA samples By Qiong Lan, Meiming Cai, Hui Xu, Xiaole Bai, Chunmei Shen, and Bofeng Zhu; Electrophoresis 2024, 45, 489–504, the authors would like to make
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A dual‐reference study design for understanding and improving AAV genome size analysis Electrophoresis (IF 2.9) Pub Date : 2024-03-16 Yali Sun, Zhi‐xiang Lu, Michael Miller, Ying Valcour, Anis H Khimani, Jana Bauer, Michael Salomon, Yanhong Tong
Recombinant adeno‐associated virus (rAAV) is the leading platform of gene delivery for its long‐lasting gene transformation and low immunogenicity. Characterization of the integrity and purity of the rAAV genome is critical to ensure clinical potency and safety. However, current rAAV genome characterization methods that can provide size assessment are either time‐consuming or not easily accessible
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Strategies to deal with genetic analyzer‐specific DNA methylation measurements Electrophoresis (IF 2.9) Pub Date : 2024-03-16 Moon Hyun So, Ji Eun Lee, Hwan Young Lee
Targeted bisulfite sequencing using single‐base extension (SBE) can be used to measure DNA methylation via capillary electrophoresis on genetic analyzers in forensic labs. Several accurate age prediction models have been reported using this method. However, using different genetic analyzers with different software settings can generate different methylation values, leading to significant errors in
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Development of an easy‐to‐set‐up multiple heart‐cutting achiral–chiral LC–LC method for the analysis of branched‐chain amino acids in commercial tablets Electrophoresis (IF 2.9) Pub Date : 2024-03-13 Ina Varfaj, Ghaid W. A. Abualzulof, Simone Moretti, Anna Migni, Irene Uda, Laura Goracci, Federica Ianni, Andrea Carotti, Roccaldo Sardella
In this paper, the development and application of a multiple heart‐cutting achiral–chiral LC–LC method (mLC–LC) for the analysis of dansylated (Dns) branched‐chain amino acids in commercial tablets are described. In the first dimension, a Waters Xbridge RP C18 achiral column was used under gradient conditions with buffered aqueous solution and acetonitrile. The elution order Dns‐valine (Dns‐Val) <
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Characterization workflow for fragments detected in capillary electrophoresis sodium dodecyl sulfate analysis of therapeutic monoclonal antibodies Electrophoresis (IF 2.9) Pub Date : 2024-03-09 Mengdan Fei, Qiang Zhang, Lei Zhang, Yueze Zhang, Lingyu Wang, Yiman Zhao, Zhongli Zhang
Product‐related fragments in monoclonal antibodies (mAbs) can have a significant impact on the efficacy and safety of the product. Capillary electrophoresis sodium dodecyl sulfate (CE‐SDS) is a commonly used method for fragment quantification, but it has challenges in peak identification due to the inability to enrich components and the incompatibility of SDS with mass spectrometry (MS). This article
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A developmental validation of the Quick TargSeq 1.0 integrated system for automated DNA genotyping in forensic science for reference samples Electrophoresis (IF 2.9) Pub Date : 2024-03-09 Junping Han, Bin Zhuang, Lixin Zou, Daoyu Wang, Li Jiang, Yi‐Liang Wei, Lijian Zhao, Lei Zhao, Caixia Li
Analysis of short tandem repeats (STRs) is a global standard method for human identification. Insertion/Deletion polymorphisms (DIPs) can be used for biogeographical ancestry inference. Current DNA typing involves a trained forensic worker operating several specialized instruments in a controlled laboratory environment, which takes 6–8 h. We developed the Quick TargSeq 1.0 integrated system (hereinafter
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Capillary electrophoresis–mass spectrometry for creatinine analysis in residual clinical plasma samples and comparison with gold standard assay Electrophoresis (IF 2.9) Pub Date : 2024-03-08 Marlien van Mever, Bingshu He, Mariam van Veen, Jeroen Slaats, Madelon M. Buijs, Joanne E. Wieringa, Thomas Hankemeier, Peter de Winter, Rawi Ramautar
When hospitalized, infants, particularly preterm, are often subjected to multiple painful needle procedures to collect sufficient blood for metabolic screening or diagnostic purposes using standard clinical tests. For example, at least 100 µL of whole blood is required to perform one creatinine plasma measurement with enzymatic colorimetric assays. As capillary electrophoresis–mass spectrometry (CE–MS)
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Enantioselective analysis of the pesticide imazamox after in vitro permeability study in Caco‐2 cells Electrophoresis (IF 2.9) Pub Date : 2024-03-08 Carolina Oliveira Serpellone, Maike Felipe Santos Barbetta, Icaro Salgado Perovani, Giulia Ballestero, Nayara Cristina Perez de Albuquerque, Anderson Rodrigo Moraes de Oliveira
Imazamox (IMX), a chiral herbicide used in cereals and oilseed crops to control weeds, is commonly sold as a racemic mixture. Its enantiomers, being chiral compounds, may exhibit unique properties when exposed to chiral environments. While IMX enantiomers have been reported to degrade differently in soil and be toxic to some species, their effects on human systems remain poorly understood. This study
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Effects and considerations of multiplexing ForenSeq Kintelligence libraries with a negative control Electrophoresis (IF 2.9) Pub Date : 2024-03-07 Gunmeet Kaur Bali, Daniela Cuenca, Jeanette Wallin
The negative template control or negative amplification control has been an essential component of the forensic DNA analysis workflow that helps monitor contamination. As such, the inclusion of a negative control in forensic DNA analysis has been a requirement for all laboratories audited under the FBI's Quality Assurance Standards. As massively parallel sequencing (MPS) becomes more conventional in
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Investigation into the optoelectrowetting droplet transport mechanism Electrophoresis (IF 2.9) Pub Date : 2024-03-02 Tao Wu, Tianyi Wang, Xiaohan He, Juncheng Zhao, Hong Yan, Teng Zhou, Liuyong Shi
To explore the optoelectronic wetting droplet transport mechanism, a transient numerical model of optoelectrowetting (OEW) under the coupling of flow and electric fields is established. The study investigates the impact of externally applied voltage, dielectric constant of the dielectric layer, and interfacial tension between the two phases on the dynamic behavior of droplets during transport. The
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Emerging use of air eDNA and its application to forensic investigations – A review Electrophoresis (IF 2.9) Pub Date : 2024-02-29 Mariya Goray, Duncan Taylor, Emily Bibbo, Chiara Fantinato, Ane Elida Fonneløp, Peter Gill, Roland A. H. van Oorschot
Biological material is routinely collected at crime scenes and from exhibits and is a key type of evidence during criminal investigations. Improvements in DNA technologies allow collection and profiling of trace samples, comprised of few cells, significantly expanding the types of exhibits targeted for DNA analysis to include touched surfaces. However, success rates from trace and touch DNA samples
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Numerical studies of manipulation and separation of microparticles in ODEP‐based microfluidic chips Electrophoresis (IF 2.9) Pub Date : 2024-02-29 Kai Zhao, Junzhu Yao, Yunman Wei, Dejian Kong, Junsheng Wang
A novel optical‐induced dielectrophoresis (ODEP) method employing a pressure‐driven flow for the continuous separation of microparticles is presented in this study. By applying alternate current electric field on conductive indium tin oxide substrate and projecting the light geometry into the photoconductive layer, an inhomogeneous electric field is locally induced. The particles experience the dielectrophoretic
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Sensitive and rapid analysis of plasmid DNA topology isoforms by capillary gel electrophoresis with laser induced fluorescence in uncoated capillary Electrophoresis (IF 2.9) Pub Date : 2024-02-28 Yan He, John Orlet, Olivia Cunio, Lawrence Thompson, Michael T Jones
The work describes the use of SYBR Gold to improve the detection sensitivity of plasmid DNA topoisomers by capillary gel electrophoresis with laser induced fluorescence in an uncoated capillary. The impact of different dyes, including ethidium bromide, SYBR Green and SYBR Gold, was compared based on detection and separation of DNA plasmid topoisomers. Use of SYBR Gold enabled improvement of detection
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Up in the air: Presence and collection of DNA from air and air conditioner units Electrophoresis (IF 2.9) Pub Date : 2024-02-28 Mariya Goray, Duncan Taylor, Emily Bibbo, Dhruvi Patel, Chiara Fantinato, Ane Elida Fonneløp, Peter Gill, Roland A. H. van Oorschot
Biological material is routinely collected at crime scenes and from exhibits and is a key type of evidence during criminal investigations. Touch or trace DNA samples from surfaces and objects deemed to have been contacted are frequently collected. However, a person of interest may not leave any traces on contacted surfaces, for example, if wearing gloves. A novel means of sampling human DNA from air
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An examination of differences in epigenetic methylation of saliva type samples based on collection method Electrophoresis (IF 2.9) Pub Date : 2024-02-22 Mirna Ghemrawi, Nicole Fernandez‐Tejero, Lia Vaquero, Amani Wanna, Justin H. Carmel, Bruce McCord
In the context of forensic casework, it is imperative to both establish a DNA profile from biological specimens and accurately identify the specific bodily fluid source. To achieve this, DNA methylation markers have been developed for the differentiation of blood, semen, vaginal epithelial secretions, and saliva samples. Saliva, alternatively referred to as oral fluid, is recognized for its heterogeneous
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Using capillary electrophoresis sodium dodecyl sulfate (CE-SDS) and liquid chromatograph mass spectrometry (LC–MS) to identify glycosylated heavy chain heterogeneity in the anti-VEGFR-2 monoclonal antibody Electrophoresis (IF 2.9) Pub Date : 2024-02-15 Meng Li, Xueyu Zhao, Gang Wu, Wenbo Wang, Jialiang Du, Gangling Xu, Maoqin Duan, Zhihao Fu, Chuanfei Yu, Lan Wang
The size variant, which can be measured by capillary electrophoresis sodium dodecyl sulfate (CE-SDS), is a critical quality attribute of monoclonal antibodies (mAbs). The CE-SDS size heterogeneity can hardly be identified by tandem mass spectrometry, which is an intractable obstacle of mAb development and quality control across the industry. We analyzed the purity of an anti-vascular endothelial growth
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Commentary regarding “An entropy model for Carreau nanofluid ciliary flow with electroosmosis and thermal radiations: A numerical study” Electrophoresis (IF 2.9) Pub Date : 2024-02-20 Asterios Pantokratoras
A serious error exists in the paper: Alharbi KAM, Riaz A, Sikandar S. An entropy model for Carreau nanofluid ciliary flow with electroosmosis and thermal radiations: a numerical study. Electrophoresis. 2024;45:https://doi.org/10.1002/elps.202300081.
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Pilot validation of on-field STR typing and human identity testing by MinION nanopore sequencing Electrophoresis (IF 2.9) Pub Date : 2024-02-14 Yuan Luo, Jiarong Zhang, Ming Ni, Zhusong Mei, Qiao Ye, Bingqian Guo, Longmei Fang, Dongyun Feng, Lu Wang, Jiangwei Yan, Guangyun Wang
Nanopore sequencing technology has broad application prospects in forensic medicine due to its small size, portability, fast speed, real-time result analysis capabilities, single-molecule sequencing abilities, and simple operation. Here, we demonstrate for the first time that nanopore sequencing platforms can be used to identify individuals in the field. Through scientific and reasonable design, a
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Analytical solutions for viscoelectric effects in electrokinetic nanochannels Electrophoresis (IF 2.9) Pub Date : 2024-02-13 Kunlin Ma, Ashwin Ramachandran, Juan G. Santiago
Understanding electrokinetic transport in nanochannels and nanopores is essential for emerging biological and electrochemical applications. The viscoelectric effect is an important mechanism implicated in the increase of local viscosity due to the polarization of a solvent under a strong electric field. However, most analyses of the viscoelectric effect have been limited to numerical analyses. In this
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Electrokinetic transport of CTAB induces multiphasic behavior during capillary adsorption and desorption Electrophoresis (IF 2.9) Pub Date : 2024-02-11 Austin S. Abrams, Alexander Eden, Bennett C. Coy, David E. Huber, Sumita Pennathur
Cationic surfactant coatings (e.g., CTAB) are commonly used in CE to control EOF and thereby improve separation efficiencies. However, our understanding of surfactant adsorption and desorption dynamics under EOF conditions is limited. Here, we apply automated zeta potential analysis to study the adsorption and desorption kinetics of CTAB in a capillary under different transport conditions: diameter
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Particle focusing mechanisms in λ-DNA solution flowing in a straight microchannel Electrophoresis (IF 2.9) Pub Date : 2024-02-11 Dalin Chen, Qiang Huang, Zhonghua Ni, Nan Xiang
Most biological fluids (such as blood, saliva, and lymph) in nature have certain viscoelasticity and are beginning to be used as the carrying fluids for viscoelastic microfluidics. However, the particle-focusing mechanisms in these new biological viscoelastic fluids are still unclear. In this work, the particle-focusing mechanisms in λ-DNA solutions were systematically explored. We first explored the
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A preliminary study characterizing temporal changes in soil bacterial communities after dismembered bones were buried Electrophoresis (IF 2.9) Pub Date : 2024-02-08 Jiaqi Wang, Zidong Liu, Jianbo Ren, Mingming Zhang, Zimeng Guan, Xingchun Zhao, Cairong Gao, Gengqian Zhang
Determining the burial time of skeletal remains is one of the most important issues of forensic medicine. We speculated that the microbiome of gravesoil may be a promising method to infer burial time by virtue of time-dependent. As we know, forensic scientists have established various models to predict the postmortem interval of a decedent based on the changes in body and soil microbiome communities
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The development of a next-generation sequencing panel targeting cannabinoid synthase genes to distinguish between marijuana and hemp Electrophoresis (IF 2.9) Pub Date : 2024-02-07 Ya-Chih Cheng, Rachel Houston
Hemp and marijuana, both derived from Cannabis sativa L. (C. sativa), are subject to divergent legal regulations due to their different Δ9-tetrahydrocannabinol (Δ9-THC) contents. Cannabinoid synthase genes are considered the key enzymes that determine the chemical composition or chemotype of a particular cultivar. However, existing methods for crop type differentiation based on previous synthase gene
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Electric manipulation on deformation of ionic ferrofluid sessile droplets Electrophoresis (IF 2.9) Pub Date : 2024-02-03 Gui-ping Zhu, Xun-an Li, Qi-yue Wang, Mei-hua Fang, Yan-chao Ding
Active electric-driven droplet manipulation in digital microfluidics constitutes a promising domain owing to the unique and programmable wettability inherent in sessile ionic droplets. The coupling between the electric field and flow field enables precise control over wetting characteristics and droplet morphology. This study delves into the deformation phenomena of ionic sessile ferrofluid droplets
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Enantioseparation of organometallic compounds by electromigration techniques Electrophoresis (IF 2.9) Pub Date : 2024-01-26 Paola Peluso, Barbara Sechi, Giorgi Jibuti
Over time, chiral organometallic compounds have attracted great interest in several fields, with applications going across several disciplines of chemical, biological, medical, and material sciences. In the last decades, due to advancements in molecular design and computational modeling, the chemistry of chiral transition metal complexes had a remarkable flowering, with the development of new structures
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Assessment of the efficiency of DNA isolation and profiling applying a temperature-driven method in human remains Electrophoresis (IF 2.9) Pub Date : 2024-01-21 Sara C. Zapico, Shayla Matos, Jillian Conte
The identification of human remains is of utmost importance in a variety of scenarios. One of the primary identification methods is DNA. DNA extraction from human remains could be difficult, particularly in situations where the remains have been exposed to environmental conditions and other insults. Several studies tried to improve extraction by applying different approaches. ForensicGEM Universal
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Holy Grail possibly made from clay Electrophoresis (IF 2.9) Pub Date : 2024-01-17 Gleb Zilberstein, Svetlana Zilberstein, Pier Giorgio Righetti
The main goal of the work was to find biochemical protein markers specific for grapes and wine in ancient amphorae shards and fermentation pools. Grape-specific proteins are more reliable markers than tartaric acid and other small organic acids (tartaric acid natural source are not only grape but also apple, mango, and other plants). The Yavne winery (located in the Central District of Israel) is stated
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CE methods for charge variant analysis of mAbs and complex format biotherapeutics Electrophoresis (IF 2.9) Pub Date : 2024-01-17 Maximilian Meudt, Martin Pannek, Nina Glogowski, Fabian Higel, Katharina Thanisch, Matthias J. Knape
Charge heterogeneity analysis of monoclonal antibodies (mAbs) and complex formats, such as bispecifics, is crucial for therapeutic applications. In this study, we developed two capillary electrophoresis (CE)-based methods, capillary zone electrophoresis (CZE) and imaged capillary isoelectric focusing (iCIEF), for analyzing a broad spectrum of mAbs and complex mAb formats. For CZE, we introduced a new
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A facile double moving redox boundary model for visual electrophoresis titration of ascorbic acid Electrophoresis (IF 2.9) Pub Date : 2024-01-16 Qian Liu, Keer Chen, Xu Xu, Qiang Zhang, Heng Liang, Chengxi Cao
In this work, we proposed a double moving redox boundary (MROB) model to realize the colorless analyte electrophoresis titration (ET) by the two steps of the redox reaction. Single MROB has been proposed for the development of ET sensing (Analyst, 2013, 138, 1137. ACS Sensor, 2019, 4, 126.), and faces great challenges in detecting the analyte without color change during redox reaction. Herein, a novel
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Capillary electrokinetic chromatography for chiral separation of potential SARS-CoV-2 3CL protease inhibitors Electrophoresis (IF 2.9) Pub Date : 2024-01-15 Lucile Brier, Christophe Furman, Julie Charton, Benoit Deprez, Emmanuelle Lipka
In this work, a capillary electrophoresis method was developed as a quality control tool to determine the enantiomeric purity of a series of five chiral compounds evaluated as potential severe acute respiratory syndrome coronavirus 2 3CL protease inhibitors. The first cyclodextrin tested, that is, highly sulfated-β-cyclodextrin, at 6% (m/v) in a 25 mM phosphate buffer, using a capillary dynamically
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On the behavior of sub-micrometer polystyrene particles subjected to AC insulator-based dielectrophoresis Electrophoresis (IF 2.9) Pub Date : 2024-01-09 Shulin Bu, Mukul Sonker, Domin Koh, Alexandra Ros
Polymer beads, especially polystyrene particles, have been extensively used as model species in insulator-based dielectrophoresis (iDEP) studies. Their use in alternating current iDEP (AC-iDEP) is less explored; however, an assessment in the low-frequency regime (≤10 kHz) allows to link surface conduction effects with the surface properties of polymer particles. Here, we provide a case study for various
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Transient dynamic electrophoresis of a soft particle Electrophoresis (IF 2.9) Pub Date : 2024-01-09 Hiroyuki Ohshima
We introduce a general theory that explains the transient dynamic electrophoresis of a soft particle in an electrolyte solution when an oscillating electric field is suddenly applied. A simple approximate analytic expression is derived for the transient dynamic electrophoretic mobility of a soft particle that can be applied to practical situations. It is found that the mobility shows a damped oscillation
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The separation of cyclic diadenosine diphosphorothioate and the diastereomers of its difluorinated derivative and the estimation of the binding constants and ionic mobilities of their complexes with 2-hydroxypropyl-β-cyclodextrin by affinity capillary electrophoresis Electrophoresis (IF 2.9) Pub Date : 2024-01-09 Sille Štěpánová, Petra Břehová, Václav Kašička
The incorporation of phosphorothioate linkages has recently been extensively employed in therapeutic oligonucleotides. For their separation and quality control, new high-efficient and high-sensitive analytical methods are needed. In this work, a new affinity capillary electrophoresis method has been developed and applied for the separation of a potential anticancer drug, 2′,3′-cyclic diadenosine diphosphorothioate
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A complete pipeline enables haplotyping and phasing macrohaplotype in long sequencing reads for polyploidy samples and a multi-source DNA mixture Electrophoresis (IF 2.9) Pub Date : 2024-01-09 Xuewen Wang, Melissa Muenzler, Jonathan King, Muyi Liu, Hongmin Li, Bruce Budowle, Jianye Ge
Macrohaplotype combines multiple types of phased DNA variants, increasing forensic discrimination power. High-quality long-sequencing reads, for example, PacBio HiFi reads, provide data to detect macrohaplotypes in multiploidy and DNA mixtures. However, the bioinformatics tools for detecting macrohaplotypes are lacking. In this study, we developed a bioinformatics software, MacroHapCaller, in which
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On the low-frequency dispersion observed in dielectrophoresis spectra Electrophoresis (IF 2.9) Pub Date : 2024-01-09 Michael Pycraft Hughes, Krista S. P. Clarke, Rashedul Hoque, Oreoluwa V. Griffiths, Emily J. Kruchek, Federico Bertagna, Kamalan Jeevaratnam, Rebecca Lewis, Fatima H Labeed
The foundation of dielectrophoresis (DEP) as a tool for biological investigation is the use of the Clausius–Mossotti (C–M) factor to model the observed behaviour of cells experiencing DEP across a frequency range. Nevertheless, it is also the case that at lower frequencies, the DEP spectrum deviates from predictions; there exists a rise in DEP polarisability, which varies in frequency and magnitude
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Investigation of interactions between biological thiols and gold nanoparticles by capillary electrophoresis with laser-induced fluorescence Electrophoresis (IF 2.9) Pub Date : 2024-01-08 Věra Dosedělová, Petr Kubáň
Biological thiols spontaneously form a stable Au–S dative bond with gold nanoparticles (AuNP) that might be used for their selective extraction and enrichment in biological samples. In this work, interactions of selected biological thiols (glutathione, cysteine, homocysteine [Hcys], cysteamine [CA], and N-acetylcysteine) with AuNP stabilized by different capping agents (citrate, Tween 20, Brij 35,
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Fully genotyping and screening of clinically important blood-group antigens by MALDI TOF mass spectrometry Electrophoresis (IF 2.9) Pub Date : 2024-01-07 Qixiu Yang, Aijing Li, Chen Wang, Jiaxuan Yang, Minghao Li, Huijun Zhu, Daru Lu, Ziyan Zhu, Luyi Ye
Several molecular biology methods are available for high-throughput blood typing. In this study, we aimed to build a high-throughput blood-group genetic screening system for high-frequency blood-group antigen-negative rare-blood groups in donors and patients. The amplification primers for all blood-type gene fragments involving the selected alleles were designed for detection. Single-base extend primers
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Enhancing the efficiency of lung cancer cell capture using microfluidic dielectrophoresis and aptamer-based surface modification Electrophoresis (IF 2.9) Pub Date : 2024-01-04 Shu-Hui Lin, Tzu-Cheng Su, Shuo Jie Huang, Chun-Ping Jen
Metastasis remains a significant cause to cancer-related mortality, underscoring the critical need for early detection and analysis of circulating tumor cells (CTCs). This study presents a novel microfluidic chip designed to efficiently capture A549 lung cancer cells by combining dielectrophoresis (DEP) and aptamer-based binding, thereby enhancing capture efficiency and specificity. The microchip features
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Unveiling facial kinship: The BioKinVis dataset for facial kinship verification and genetic association studies Electrophoresis (IF 2.9) Pub Date : 2023-12-31 Jian Yu, Xiaozhe Jin, Weijie Du, Yantong Bai, Xin Zhou, Mengli Gao, Shuwen Li, Jiarui Qin, Xuanlong Chen, Yuhao Liu, Jianing Yu, Chen Chen, Qiheng Xie, Sumei Xie, Xiaochao Kong, Wenxuan Zhan, Yanfang Yu, Kai Li, Qiang Ji, Feng Chen, Peng Chen
Facial image–based kinship verification represents a burgeoning frontier within the realms of computer vision and biomedicine. Recent genome-wide association studies have underscored the heritability of human facial morphology, revealing its predictability based on genetic information. These revelations form a robust foundation for advancing facial image–based kinship verification. Despite strides
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High-efficiency extraction of target particles in viscoelastic contraction–expansion microchannels Electrophoresis (IF 2.9) Pub Date : 2023-12-31 Bin Wu, Shaowei Liu, Di Jiang, Wenlai Tang
The efficient and precise extraction of target particles is a crucial prerequisite for achieving accurate detection and analysis in microfluidic cell analysis. In this study, a symmetrical contraction–expansion microchannel with sheath flow was designed, aiming to extract target larger particles from particles of different sizes within the channel. This paper conducted numerical simulations to investigate
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Multilayer track-etched membrane-based electroosmotic pump for drug delivery Electrophoresis (IF 2.9) Pub Date : 2023-12-31 Qian Yang, Zebo Zhang, Junshu Lin, Boyu Zhu, Rongying Yu, Xinru Li, Bin Su, Bo Zhao
Herein, we report an electroosmotic pump (EOP) based on a multilayer track-etched polycarbonate (PC) membrane. A remarkable increase of maximum backpressure (198.2–2400 mmH2O) of a fundamental pump unit was obtained at 0.8 mA, when the number of PC membranes was increased from 1 to 10. Meanwhile, the corresponding flow rate was increased from 80.3 to 111.7 µL/min. Furthermore, multiple pump units were
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A bioinspired approach for the modulation of electroosmotic flow and protein–surface interactions in capillary electrophoresis using silylated amino-amides blocks and covalent grafting Electrophoresis (IF 2.9) Pub Date : 2023-12-31 Jérémie Gouyon, Margaux Clavié, Gutiérrez-Climente Raquel, Giang Ngo, Pascal Dumy, Pascal Etienne, Pierre Martineau, Martine Pugnière, Mehdi Ahmad, Gilles Subra, Catherine Perrin, Yoann Ladner
We explore a bioinspired approach to design tailored functionalized capillary electrophoresis (CE) surfaces based on covalent grafting for biomolecules analysis. First, the approach aims to overcome well-known common obstacles in CE protein analysis affecting considerably the CE performance (asymmetry, resolution, and repeatability) such as the unspecific adsorption on fused silica surface and the
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Modulating selective ionic enrichment and depletion zones in straight nanochannels via the interplay of surface charge modulation and electric field mediated fluid-thickening Electrophoresis (IF 2.9) Pub Date : 2023-12-24 Anindita Bhattacharya, Suman Chakraborty
We report the possibilities of achieving highly controlled segregation of ion-enriched and ion-depleted regions in straight nanochannels. This is achieved via harnessing the interplay of an axial gradient of the induced transverse electric field on account of electrical double layer phenomenon and the localized thickening of the fluid because of intensified electric fields due to the large spatial
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Entropy generation in a ciliary flow of an Eyring–Powell ternary hybrid nanofluid through a channel with electroosmosis and mixed convection Electrophoresis (IF 2.9) Pub Date : 2023-12-19 Nawal A. Alshehri, Arshad Riaz, Sheraz Sikandar, Taseer Muhammad
Drug delivery systems, where the nanofluid flow with electroosmosis and mixed convection can help in efficient and targeted drug delivery to specific cells or organs, could benefit from understanding the behavior of nanofluids in biological systems. In current work, authors have studied the theoretical model of two-dimensional ciliary flow of blood-based (Eyring–Powell) nanofluid model with the insertion
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Numerical and experimental investigation of the deviation of microparticles inside the microchannel using the vortices caused by the ICEK phenomenon Electrophoresis (IF 2.9) Pub Date : 2023-12-19 Seyed Mohammad Ehsan Ghadamgahi, Mohammad Mohsen Shahmardan, Mohsen Nazari, Hamed Mansouri, Nicole N Hashemi
One field of study in microfluidics is the control, trapping, and separation of microparticles suspended in fluid. Some of its applications are related to cell handling, virus detection, and so on. One of the new methods in this field is using ICEK phenomena and dielectrophoresis forces. In the present study, considering the ICEK phenomena, the microparticles inside the fluid are deviated in the desired
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Mobilization in two-step capillary isoelectric focusing: Concepts assessed by computer simulation Electrophoresis (IF 2.9) Pub Date : 2023-12-19 Wolfgang Thormann, Richard A. Mosher
The mobilization step in a two-step capillary isoelectric focusing protocol is discussed by means of dynamic computer simulation data for systems without and with spacer compounds that establish their zones at the beginning and end of the focusing column. After focusing in an electroosmosis-free environment (first step), mobilization (second step) can be induced electrophoretically, by the application
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Front Cover: Purification of a recombinant oncolytic virus from clarified cell culture media by anion exchange monolith chromatography Electrophoresis (IF 2.9) Pub Date : 2023-12-13 Troy Rogerson, Guoling Xi, Amanda Ampey, Jon Borman, Sally Jaroudi, Dan Pappas, Thomas Linke
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