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Enhancement of Oral Bioavailability of Protein and Peptide by Polysaccharide-based Nanoparticles Protein Pept. Lett. (IF 1.6) Pub Date : 2024-03-21 Md. Moidul Islam, Sarjana Raikwar
: Oral drug delivery is a prevalent and cost-effective method due to its advantages, such as increased drug absorption surface area and improved patient compliance. However, delivering proteins and peptides orally remains a challenge due to their vulnerability to degradation by digestive enzymes, stomach acids, and limited intestinal membrane permeability, resulting in poor bioavailability. The use
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In Silico Analysis of Natural Plant-Derived Cyclotides with Antifungal Activity against Pathogenic Fungi Protein Pept. Lett. (IF 1.6) Pub Date : 2024-03-06 Akshita Sharma, Bisma Butool, Pallavi Sahu, Reema Mishra, Aparajita Mohanty
Background: Fungal infections in plants, animals, and humans are widespread across the world. Limited classes of antifungal drugs to treat fungal infections and loss of drug efficacy due to rapidly evolving fungal strains pose a challenge in the agriculture and health sectors. Hence, the search for a new class of antifungal agents is imperative. Cyclotides are cyclic plant peptides with multiple bioactivities
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Interleukin 6 Antagonists in Severe COVID-19 Disease: Cardiovascular and Respiratory Outcomes Protein Pept. Lett. (IF 1.6) Pub Date : 2024-02-20 Shahzad Khan
Background:: Inhibitors of interleukin 6 [IL-6] have been utilized to treat severe COVID-19 disease. Their immunosuppressive or immunomodulating impact may be beneficial in COVID-19. Objectives:: To discuss the role of IL-6 inhibitors and assess various trials conducted to evaluate the efficacy of IL-6 inhibitors in COVID-19 disease. Summary:: Two of the most common causes of mortality in COVID-19-infected
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Characterization of Heat-labile Uracil-DNA Glycosylase from Oncorhynchus mykiss and its Application for Carry-over Contamination Control in RT-qPCR Protein Pept. Lett. (IF 1.6) Pub Date : 2024-02-12 Qingyuan Huang, Yaqi Zhang, Wenhao Hu, Keqi Chen, Jian Zhang, Zhidan Luo, Chen Lu
Background: Heat-labile uracil-DNA glycosylase (HL-UDG) is commonly employed to eliminate carry-over contamination in DNA amplifications. However, the prevailing HL-UDG is markedly inactivated at 50°C, rendering it unsuitable for specific one-step RT-qPCR protocols utilizing reverse transcriptase at an optimal temperature of 42°C. Objective: This study aimed to explore novel HL-UDG with lower inactivation
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Prokaryotic Expression and Affinity Purification of DDX3 Protein Protein Pept. Lett. (IF 1.6) Pub Date : 2024-02-02 Lan Huang, Yue Liang, Huijin Hou, Min Tang, Xinpeng Liu, Yan-ni Ma, Shufang Liang
Background: DDX3 is a protein with RNA helicase activity that is involved in a variety of biological processes, and it is an important protein target for the development of broad-spectrum antiviral drugs, multiple cancers and chronic inflammation. Objective: The objective of this study is to establish a simple and efficient method to express and purify DDX3 protein in E. coli, and the recombinant DDX3
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P53-induced GAP-43 Upregulation in Primary Cortical Neurons of Rats Protein Pept. Lett. (IF 1.6) Pub Date : 2024-01-29 Tianxia Li, Yuexin Jia, Junxian Fu, Zhuo Fu, Zhidong Qiao, Xiaoyang Liu, Ting Lv, Rong Tang, Guanglu Yang
Objective: In this study, we employed an in-vitro culturing technique to investigate the impact of p53 on the modulation of growth-associated protein-43 (GAP-43) within the primary cortical neurons of rat specimens. Methods: 1) Within the first 24 hours after birth, the bilateral cortex was extracted from newborn Wistar rats and primary cortical neurons were cultured and identified. 2) The changes
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Characterization of a Novel Thermostable 7α-Hydroxysteroid Dehydrogenase Protein Pept. Lett. (IF 1.6) Pub Date : 2024-01-26 Deshuai Lou, Yangyang Cao, Hongtao Duan, Jun Tan, Binyan Li, Yuanjun Zhou, Dong Wang
Background: 7α-Hydroxysteroid dehydrogenase (7α-HSDH) plays a pivotal role in vivo in the biotransformation of secondary bile acids and has great potential in industrial biosynthesis due to its broad substrate specificity. In this study, we expressed and characterized a novel thermostable 7α-HSDH (named Sa 7α-HSDH). Methods: The DNA sequence was derived from the black bear gut microbiome metagenomic
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Molecular Machinery of the Triad Holin, Endolysin, and Spanin: Key Players Orchestrating Bacteriophage-Induced Cell Lysis and their Therapeutic Applications Protein Pept. Lett. (IF 1.6) Pub Date : 2024-01-22 Safia Samir
Phage therapy, a promising alternative to combat multidrug-resistant bacterial infections, harnesses the lytic cycle of bacteriophages to target and eliminate bacteria. Key players in this process are the phage lysis proteins, including holin, endolysin, and spanin, which work synergistically to disrupt the bacterial cell wall and induce lysis. Understanding the structure and function of these proteins
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Improving Photocleavage Efficiency of Photocleavable Protein for Antimicrobial Peptide Histatin 1 Expression Protein Pept. Lett. (IF 1.6) Pub Date : 2024-01-12 Nana Zhou, Tai An, Yuan Zhang, Guomiao Zhao, Chao Wei, Xuemei Shen, Fan Li, Xiaoyan Wang
Background: Antimicrobial peptides (AMPs) are promising alternative agents for antibiotics to overcome antibiotic resistance problems. But, it is difficult to produce large-scale antimicrobial research due to the toxicity towards expression hosts or degradation by peptidases in the host. Therefore, heterologous recombinant expression of antimicrobial peptides has always been a challenging issue. Objectives:
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Feasibility of Domain Segmentation of B19V VP1u Using Intein Technology for Structural Studies Protein Pept. Lett. (IF 1.6) Pub Date : 2024-01-12 Renuk Varayil Lakshmanan, Mavis Agbandje-McKenna, Robert McKenna
Introduction: Parvovirus B19 (B19V) is a human pathogen, and the minor capsid protein of B19V possesses a unique N terminus called VP1u that plays a crucial role in the life cycle of the virus. Objectives: The objective of this study was to develop a method for domain segmentation of B19 VP1u using intein technology, particularly its receptor binding domain (RBD) and phospholipase A2 (PLA2) domain
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Advances in Research on Protein Arginine Methyltransferase 2: Functions and Diseases Protein Pept. Lett. (IF 1.6) Pub Date : 2023-12-28 Zhen-Qi Min, Ming-Jun Jiang, Xi-Lian Liu, Su-Peng Yuan, Ping-An Chen, Chu-Hao Wang, Ya-Jun Chen, Xian-Peng Dai
Protein arginine methylation stands as a prevalent post-translational modification process, exerting vital roles in cellular signal transduction, gene expression, and cell cycle regulation. Amidst the protein arginine methyltransferase (PRMT) family, PRMT2 stands as a less explored constituent. Nonetheless, its regulatory roles in transcriptional regulation, post-transcriptional modification, methylation
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VEGF165b Mutant Promotes the Apoptosis of Murine Breast Cancer Cells Induced by Paclitaxel by Inducing Tumor Vessel Maturation Protein Pept. Lett. (IF 1.6) Pub Date : 2023-12-19 Chen Liang, Youwei Li, Enhui Guo, Shuge Bai, Yan Wang, Huiyong Zhang
Introduction: The anti-angiogenic agent vascular endothelial growth factor 165b (VEGF165b) mutant (mVEGF165b), which was developed by our laboratory, has superior antitumor activity to that of native VEGF165b; however, its mechanism of action and druggability need further exploration. Methods: Using the commercial anti-angiogenic drug bevacizumab as a positive control, the mechanism and developability
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Dexmedetomidine Alleviates Brain Ischemia/Reperfusion Injury by Regulating Metastasis-associated Lung Adenocarcinoma Transcript 1/MicroRNA-140-5p/ Nuclear Factor Erythroid-derived 2-like 2 Axis Protein Pept. Lett. (IF 1.6) Pub Date : 2023-12-11 Zhigang Qin, Younian Xu
Background: Dexmedetomidine (Dex) is widely used in perioperative anesthesia, and recent studies have reported that it protects organs from ischemia/reperfusion (I/R) injury. Objectives: This study was performed to investigate the role of Dex in alleviating cerebral I/R injury and its regulatory effects on metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)/microRNA-140-5p (miR-140-5p)/nuclear
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LINC01836 Promotes Colorectal Cancer Progression and Functions as ceRNA to Target SLC17A9 by Sponging miR-1226-3p Protein Pept. Lett. (IF 1.6) Pub Date : 2023-12-06 Zhihua Xu, Yue Yu, Hao Ni, Wei Sun, Yuting Kuang
Background: Increasing evidence proves that long non-coding RNAs (lncRNAs) play a key role in the occurrence and development of colorectal cancer. However, the function and molecular mechanism of LINC01836 in CRC are still unknown. Methods: The differentially expressed lncRNAs in colorectal cancer were obtained from the RNA sequencing data. The effects of LINC01836 on colorectal cancer cells were tested
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Exploring Potential Biomarkers of Early Thymoma based on Serum Proteomics Protein Pept. Lett. (IF 1.6) Pub Date : 2023-12-04 Min Jin, Peng Liu, Guoyan Qi
Background: Early diagnosis remains difficult because the early symptoms of thymoma are atypical. Objective: This study aimed to analyze the changes of serum proteins in the early stage of thymoma (stage I/II) by proteomics method and to screen and validate candidate biomarkers. Methods: Proteins were extracted from 8 sera patients with stage I/II thymoma and 9 healthy controls. The levels of serum
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Structural Shifts of the Parvovirus B19 Capsid Receptor-binding Domain: A Peptide Study Protein Pept. Lett. (IF 1.6) Pub Date : 2023-12-04 Vladislav Victorovich Khrustalev, Aleksander Nicolaevich Stojarov, Anastasia Aleksandrovna Akunevich, Oleg Evgenyevich Baranov, Anna Vladimirovna Popinako, Elena Olegovna Samoylovich, Marina Anatolyevna Yermolovich, Galina Valeryevna Semeiko, Egor Gennadyevich Sapon, Victoria Igorevna Cheprasova, Nikolai Vladimirovich Shalygo, Victor Vitoldovich Poboinev, Tatyana Aleskandrovna Khrustaleva, Olga Victorovna
Background: Binding appropriate cellular receptors is a crucial step of a lifecycle for any virus. Structure of receptor-binding domain for a viral surface protein has to be determined before the start of future drug design projects. Objectives: Investigation of pH-induced changes in the secondary structure for a capsid peptide with loss of function mutation can shed some light on the mechanism of
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KIF20A Promotes CRC Progression and the Warburg Effect through the C-Myc/HIF-1α Axis Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-30 Min Wu, Xianqiang Wu, Jie Han
Background: Colorectal cancer (CRC) is a prevalent form of cancer globally, characterized by a high mortality rate. Therefore, discovering effective therapeutic approaches for CRC treatment is critical. Methods: The levels of KIF20A in CRC clinical samples were determined using Western Blot and immunofluorescence assay. SW480 cells were transfected with siRNA targeting KIF20A, while HT-29 cells were
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MicroRNA3650 Promotes Gastric Cancer Proliferation and Migration through the PTEN/PI3K-AKT-mTOR and Hippo Pathways Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-30 Xiansheng Yang, Juncai Wen, Qingjun He, Shuoshan Wang, Qiang Ruan, Quanxing Liao, Jinfu He, Shuxian Fang, Chang Liu, Hongsheng Tang
Background:: Gastric cancer (GC) is a malignant tumor with seriously poor outcomes. Studies have shown that microRNAs (miRNAs) play an omnifarious regulatory effect in GC. However, the role of miR-3650 in the progression of GC is not well known. Methods:: In this study, miR-3650 expression and its clinical significance were determined using clinical specimens. The biological functions of miR-3650 were
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The Role of circRNA-miRNA-mRNA Regulatory Network and its Potential Biomarker Function in Colorectal Cancer Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-29 Xutang Fu, Pengpeng Chen, Hao Wang
Background: Revealing the process and mechanism of colorectal cancer will facilitate the discovery of new biomarkers and contribute to the development of targeted drugs. Objective: This study aimed to explore the potentially functional circRNA-miRNA-mRNA network in colorectal cancer (CRC), and further explore its mechanism. Methods: Bioinformatics analysis was used to identify the differentially expressed
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Antiviral Peptides Derived from Plants: Their Designs and Functions Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-27 Haleh Feyzyab, Nikoo Fathi, Azam Bolhassani
In recent years, plant-derived bioactive compounds have been developed as antiviral agents. Plants synthesize a variety of compounds, especially peptides, which possess antimicrobial activity. Current studies have shown that some antimicrobial peptides have antiviral activity against a wide range of human DNA and RNA viruses and play an effective role in the treatment of human viral diseases. These
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Unleashing Breast Cancer Progression: miR-455-5p's Targeting of SOCS3 Drives Proliferation, Migration, and Invasion Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-27 Xin Li, Bing Peng, Jian Li, Mi Tian, Lili He
Objective: We aim to investigate the regulatory mechanisms of miR-455-5p/SOCS3 pathway that underlie the proliferation, migration, and invasion of triple-negative breast cancer (TNBC) cells. Methods: Reverse transcription-quantitative PCR (RT-qPCR) was used to detect miR-455-5p expression in breast cancer tissues and cell lines. CCK8 and Transwell assays were conducted to assess the effects of miR-455-5p
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Pharmacological Classes of Conus Peptides Targeted to Calcium, Sodium, and Potassium Channels Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-27 Elsie C. Jimenez
: This review describes the specific features of families of Conus venom peptides (conotoxins or conopeptides) that represent twelve pharmacological classes. Members of these conopeptide families are targeted to voltage-gated ion channels, such as calcium, sodium, and potassium channels. The conopeptides covered in this work include omega-conotoxins and contryphans with calcium channels as targets;
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STAM-binding Protein-like 1 Promotes Growth and Migration of Colorectal Cancer by NF-κB Pathway Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-24 Xinghua Zhou, Yue Cheng, Jian Kang, Gang Mao
Background: STAM-binding protein-like 1 (STAMBPL1) functions as a deubiquitinase to cleave Lys63 ubiquitin linkage, and is associated with cancer dissemination and progression. The role of STAMBPL1 in colorectal cancer (CRC) remains unclear. Methods: STAMBPL1 expression was determined by western blot and qRT-PCR. Cell proliferation was detected by colony formation and MTT assays, and apoptosis was
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Investigation of Early Growth Response Protein [EGR] Levels in Patients with Fibromyalgia Syndrome: A Cross-Sectional Study Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-24 Ayca Tas, Tugba Agbektas, Ahmet Karadag, Süleyman Aydin, Emrullah Hayta, Yavuz Silig
Background: Fibromyalgia is a soft tissue rheumatism characterized by chronic and widespread musculoskeletal pain at specific points in the body. Objectives: In this study, we aimed to investigate the relationship between Early Growth Response (EGR1, EGR2, and EGR3) protein levels in patients with Fibromyalgia Syndrome (FMS) and healthy controls. Methods: In our studies, 76 FMS patient group and 78
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The Implication of Hypocretin in Drug Abuse and Arousal in the Brain Stem Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-24 Masoumeh Kourosh Kourosh-Arami, Alireza Komaki, Masoumeh Gholami
Hypocretin (orexin, Hcrt) neurons located in the lateral hypothalamus (LH) project widely into the brain and are thus responsible for the physiological action of the hypocretin complex. Hypocretin is involved in both arousal and addiction, and brainstem areas such as the locus coeruleus (LC), paragigantocellularis (PGi), and dorsal raphe (DR) contribute to these functions. In the present review, we
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The Function and Modification of Human Defensin 5 Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-24 Xin-Yue Chang, Meng-Wei Zhang, Lin-Jie Zhang, Lian-Qin Chai
: The antibacterial and antiviral functions of human defensin 5 lay the foundation for its role as a core host protective component. In addition, HD5 also has the function of inhibiting tumor proliferation and immune regulation. However, everything has two sides; cytotoxic and proinflammatory properties may exist, while HD5 performs physiological functions. Accordingly, the modification and engineering
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Biomarker of Pulmonary Inflammatory Response in LUAD: miR-584-5p Targets RAB23 to Suppress Inflammation Induced by LPS in A549 Cells Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-24 Enyu Yang, Yinuo Hong, Cheng Xuan, Juan Xu, Qianyun Ding, Shuo Zhao, Haihan Ye, Xiaowei Fan, Zhenggang Jiang, Siquan Zhang, Xianfeng Ding
Background: Pulmonary inflammatory response (PIR) is one of the prognostic risk factors of lung adenocarcinoma (LUAD), with a high mortality rate. Objective: This study aims to investigate prognostic microRNA (miRNA) to improve clinical prognosis prediction and postoperative inflammation treatment in LUAD patients. Method: About 201 differentially expressed microRNAs (DE-miRNAs) in LUAD were mined
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Water-soluble Moringa oleifera Seed Lectin Exhibits Monoaminergic Pathway-linked Anti-depressive-like Effects in Mice Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-21 Leydianne Patriota, Barbara Lima, Amanda Marinho, Jainaldo Costa, Luana Coelho, Moacyr Rêgo, Maira Pitta, Patrícia Paiva, Michelly Pereira, Thiago Napoleão, Michelle Rosa
Objectives: The present study investigated the anti-depressive-like (anti-immobility) effect of a lectin from Moringa oleifera seeds (WSMoL) in mice. Methods: To evaluate an acute effect, the animals were treated with WSMoL (1, 2, and 4 mg/kg, i.p.) 30 min before the tail suspension test (TST). To investigate the involvement of monoaminergic and nitrergic signaling, the mice were pre-treated with selective
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A Review of Gap Junction Protein and its Potential Role in Nervous System-Related Disease Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-17 Qiong Xiang, Xiao-Lin Liu, Jia-Jia Chen, Liang Yang, Li-Ni Liu, Jing Deng, jia-sheng tao, Xian-Hui Li
: Gap junction (GJ) is a special cell membrane structure composed of connexin. Connexin is widely distributed and expressed in all tissues except differentiated skeletal muscle, red blood cells, and mature sperm cells, which is related to the occurrence of many genetic diseases due to its mutation. Its function of regulating immune response, cell proliferation, migration, apoptosis, and carcinogenesis
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Physiological and Pathological Roles of NTSR2 in Several Organs and Diseases (Review) Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-14 Yuting Yang, Wenxin Zhang, Kun Wei, Fei Hu, Song Wu, Yuan Ma, Qing Ouyang
Neurotensin (NTS) and its receptors (NTSRs) have long been the subject of study and have shown to have a vital function in a variety of systems. They are specifically implicated in the development of tumors and have both oncogenic and anti-apoptotic effects. Neurotensin receptor 2 (NTSR2), like NTSR1, belongs to the G protein-coupled receptor family and has been linked to analgesia, mental disorders
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Exploring the Cell Wall and Secretory Proteins of Mycobacterium leprae as Biomarkers Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-13 Sakshi Singh, Devesh Sharma, Sakshi Gautam, Mamta Arora, Deepa Bisht
The bacterial cell wall is composed of a wide variety of intricate proteins in addition to lipids, glycolipids, and polymers. Given the diversity of cell wall proteins among bacterial species, they are a feasible target for biomarker identification and characterization in clinical research and diagnosis of the disease. The slow growth rate of Mycobacterium leprae poses a major hurdle in the accurate
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Novel Circular RNA CircUBAP2 Drives Tumor Progression by Regulating the miR-143/TFAP2B Axis in Prostate Cancer Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-13 Zhong Lv, Yunfeng Shi, Haoran Wu, Kai Cao, Xiaowu Liu, Chengyue Wang
Background: More and more investigations reveal that circular RNAs (circRNAs) are involved in cancer progression. CircRNA UBAP2 was closely related to prostate cancer. However, the biological function and specifical mechanism of circUBAP2 are still poorly discovered in prostate cancer (PCa). Objective: This study aims to explore the biological function and mechanism of circUBAP2 in PCa. Methods: The
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Combining Network Pharmacology, Molecular Docking and Preliminary Experiments to Explore the Mechanism of Action of FZKA Formula on Non-small Cell Lung Cancer Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-13 Zhuixing Liu, Jie Zhang, Jinpeng Liu, Lihong Guo, Guangwei Chen, Yu Fang, Yang Yang
Background: Clinically, Fuzhengkangai formulation (FZKA) has been proven to have significant therapeutic effects on non-small lung cancer (NSCLC), although the mechanism is unknown. We aimed to explore the potential mechanism of FZKA in the treatment of NSCLC in this study. Methods: We obtained the active components and targets of FZKA by TCMSP. The target genes of NSCLC were searched from OMIM, GEO
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First display of Haloalkane Dehalogenase LinB on the Surface of Bacillus subtilis Spore Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-10 Fuli Wang, Xiujie Liu, Tianyu Song, Chengxin Pei, Qibin Huang, Hui Jiang, Hailing Xi
Background: LinB, as a Haloalkane dehalogenase, has good catalytic activity for many highly toxic and recalcitrant compounds, and can realize the elimination of chemical weapons HD in a green non-toxic mode. Objective: In order to display Haloalkane dehalogenase LinB on the surface of Bacillus subtilis spore Method: We have constituted the B. subtilis spore surface display system of halogenated alkanes
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Crystal Structure Determination of Nucleotide-sugar Binding Domain of Human UDP-glucuronosyltransferases 2B10 Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-10 Xinli Yin, Xi Lu, Xudan Qi, Yuxi Tu, Na Zhang, Yuan Yang, Xiabin Chen, Junsen Tong
Background: UDP-glucuronosyltransferases (UGTs) play a crucial role in maintaining endobiotic homeostasis and metabolizing xenobiotic compounds, particularly clinical drugs. However, the detailed catalytic mechanism of UGTs has not been fully elucidated due to the limited availability of reliable protein structures. Determining the catalytic domain of human UGTs has proven to be a significant challenge
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Oxidative Stress in Beta-thalassemia Patients: Role of Enzymatic and Non-enzymatic Modulators Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-10 Tehseen Fatima, Sadaf Khan, Muhammad Mubashir Khan, Rameesha Kamran, Muhammed Wajih Uddin, Saba Sohrab
Background: Oxidative stress is a pathophysiological state that arises due to an imbalance created between ROS generation and the antioxidant potential of the host cell. Transfusion- dependent beta-thalassemia major patients are at high risk of cellular and molecular damages induced by ROS mainly due to iron overload caused by repetitive blood transfusion. Objectives: To analyze oxidative stress status
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Angiotensin-(1-7) Improves Islet β-cell Dedifferentiation by Activating PI3K/Akt/FoxO1 Pathway Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-10 Hao Guo, Dandan Guo, Min An, Ruonan Zhang, Caixia Wang, Junhua He
Background: Islet β-cell dedifferentiation may be the main cause of reduced insulin secretion. Angiotensin-(1-7) [Ang-(1-7)] can attenuate high glucose-induced apoptosis and dedifferentiation of pancreatic β-cell, but the specific signal transduction pathway and mechanism are not yet clear. Objective: This study aimed to investigate the effects of Ang-(1-7) on high glucose-induced islet β-cell dedifferentiation
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An Insight into the Protein Aggregation in Alzheimer’s Disease and its Inhibition Protein Pept. Lett. (IF 1.6) Pub Date : 2023-11-10 Abdul Basit Khan, Rizwan Hasan Khan
: Alzheimer’s disease, a neurodegenerative disease, is a progressive and irreversible disease that has become a global challenge due to its increasing prevalence and absence of available potential therapies. Protein misfolding and aggregation are known to be the root of several protein neurodegenerative diseases, including Alzheimer’s disease. Protein aggregation is a phenomenon where misfolded proteins
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Evaluation of Cytotoxicity and Antimicrobial Activity of Experimental Composites Containing Chitosan-Silver Oxide Particles Against Two Main Pathogenic Bacteria in Periodontal Disease Protein Pept. Lett. (IF 1.6) Pub Date : 2023-10-25 Nahid Nasrabadi, Navid Ramezanian, Parisa Ghorbanian, Ali Forouzanfar, Hamideh Sadat Mohammadipour
Introduction: Bacterial biofilm is known as the main cause of periodontal disease. Generally, the anaerobic Gram-negative, such as Porphyromonas gingivalis and Fusobacterium nucleatum, are considered the most identified bacteria. Objective: This study aimed to investigate the antimicrobial effect and cytotoxicity of two experimental composites containing chitosan-silver oxide (CH-Ag2O) particles. Materials
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Activation and Denitrosylation of Procaspase-3 in KA-induced Excitotoxicity Protein Pept. Lett. (IF 1.6) Pub Date : 2023-10-25 Yong Liu, Hui Yan, Jia Zhang, Yu-Ting Cai, Xiao-Hui Yin, Feng Lu, Ying-Kui Liu, Chong Li
Background: It has been reported that activation of glutamate kainate receptor subunit 2 (GluK2) subunit-containing glutamate receptors and the following Fas ligand(FasL) up-regulation, caspase-3 activation, result in delayed apoptosis-like neuronal death in hippocampus CA1 subfield after cerebral ischemia and reperfusion. Nitric oxide-mediated S-nitrosylation might inhibit the procaspase activation
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Hsa_circ_0001687 Function as a ceRNA to Facilitate Hepatocellular Carcinoma Progression via miR-140- 3p/FOXQ1 Axis Protein Pept. Lett. (IF 1.6) Pub Date : 2023-10-23 Song You, Niangmei Cheng, Fei Wang, Xiaoyuan Zheng, Yingchao Wang, Bixing Zhao, Jingfeng Liu
Background: Increasingly convincing evidence has revealed that circular RNAs (circRNAs) are critical regulatory components of hepatocellular carcinoma (HCC) genesis. However, the expression of circRNAs in HCC and the relevance of circRNAs to HCC progression remain largely unexplained. Methods: qRT-PCR or western blotting was utilized to confirm circ_0001687, miR-140-3p, and Forkhead Box q1 (FOXQ1)
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Molecular Bases of Protein Antigenicity and Determinants of Immunogenicity, Anergy, and Mitogenicity Protein Pept. Lett. (IF 1.6) Pub Date : 2023-10-16 David Pedroza-Escobar, Irais Castillo-Maldonado, Tania González-Cortés, Dealmy Delgadillo-Guzmán, Pablo Ruíz-Flores, Jorge Haro Santa Cruz, Perla-Karina Espino-Silva, Erika Flores-Loyola, Agustina Ramirez-Moreno, Joaquín Avalos-Soto, Miguel-Ángel Téllez-López, Sergio-Everardo Velázquez-Gauna, Rubén García-Garza, Rubén Daniel Arellano Pérez Vertti, Cristian Torres-León
Background: The immune system is able to recognize substances that originate from inside or outside the body and are potentially harmful. Foreign substances that bind to immune system components exhibit antigenicity and are defined as antigens. The antigens exhibiting immunogenicity can induce innate or adaptive immune responses and give rise to humoral or cell-mediated immunity. The antigens exhibiting
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Clay-Polymer Nanocomposites Mediated Inhibition of Protein Aggregation: Possible Role in the Prevention of Proteinopathies Protein Pept. Lett. (IF 1.6) Pub Date : 2023-10-15 Romana Parveen, Sher Ali, Sadaf Fatima
Background: The transformation of proteins from their native conformation into highly ordered fibrillar structures due to their misfolding and aggregation under particular conditions are described as beta-sheet enriched amyloid fibrils. The accumulation of these fibrils in different body parts is the major cause of several neurological and non-neurological conditions (proteinopathies). Objectives:
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Challenges and Solutions in the Recombinant Expression of Membrane Proteins Protein Pept. Lett. (IF 1.6) Pub Date : 2023-10-10 Caijuan Liu, Hanxue He, Jie Tian, Yunqi Ma
Membrane proteins are important components of the proteome and play key roles in many biological processes, such as signal transduction, material transport, cell recognition, etc. Membrane proteins are involved in several fields, and more and more researchers want to understand them. However, the structural properties of membrane proteins make their recombinant expression yield low. This adversely
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A Novel Brevinin2 HYba5 Peptide against Polymicrobial Biofilm of Staphylococcus aureus and Enterococcus faecalis Protein Pept. Lett. (IF 1.6) Pub Date : 2023-10-10 Megha Periyappilly Radhakrishnan, Karthika Suryaletha, Iype Joseph, Sanil George, Sabu Thomas
Background: Brevinin2 HYba5 (Peptide 29) is a novel cationic peptide identified from an endemic frog, Hydrophylax bahuvistara. Staphylococcus aureus and Enterococcus faecalis are troublesome biofilm-forming pathogens associated with nosocomial and community-acquired infections and contribute to the severity of infections associated with implanted devices and chronic wounds. Co-existence of both pathogens
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Proteomic Analysis by 4D Label-free MS-PRM Provides Insight into the Role and Regulatory Mechanisms of IL-25 in NK Cells Protein Pept. Lett. (IF 1.6) Pub Date : 2023-10-04 Juan Feng, Luoyao Huang, Shuaipeng Yang, Jiasheng Pan, Xiangxing Zhu, Dongsheng Tang
Background: NK cells play an important role in immune response, immune surveillance, and metabolism regulation. Therefore, NK cells are involved in the occurrence and development of various diseases, such as infectious diseases, cancer, obesity, and diabetes. IL-25 is a special member of the IL-17 family with anti-inflammatory function. IL-25 can regulate inflammatory response and metabolism via various
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Expression and Prognostic Significance of Ferroptosis-related Proteins SLC7A11 and GPX4 in Renal Cell Carcinoma Protein Pept. Lett. (IF 1.6) Pub Date : 2023-10-02 Zongtao Ren, Xiaoyu Zhang, Jingya Han
Background: The ferroptosis inhibitory gene solute carrier family 7 member 11 (SLC7A11) and glutathione peroxidase 4 (GPX4) inhibit ferroptosis in carcinoma cells. However, whether SLC7A11 and GPX4 serve as an oncogene in renal cell carcinoma (RCC) remains unclear. Methods: Immunohistochemistry (IHC) assays were performed to assess the expression of SLC7A11 and GPX4 in human RCC tissues. Clinical-pathological
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Exogenous Apelin-13 Administration Ameliorates Cyclophosphamide- Induced Oxidative Stress, Inflammation, and Apoptosis in Rat Lungs Protein Pept. Lett. (IF 1.6) Pub Date : 2023-09-22 Arzu Keskin-Aktan, Özden Kutlay
Background: Apelin-13 is an endogenous adipocytokine known for its antioxidant, antiinflammatory, and antiapoptotic properties. Objective: We aimed to investigate the possible protective effects of exogenous Apelin-13 administration on oxidative stress, inflammation, and apoptosis induced by the cytotoxic agent cyclophosphamide (CP) in the lungs. Methods: Twenty-four male Wistar albino rats were divided
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Structural and Functional Analysis of Urease Accessory Protein E from Vancomycin-Resistance Staphylococcus aureus MU50 Strain Protein Pept. Lett. (IF 1.6) Pub Date : 2023-09-15 Humaira Siddiqui, Atia-tul-Wahab, Aftab Ahmed, M. Iqbal Choudhary
Background: An increasing prevalence of biofilm forming strains by vancomycinresistance Staphylococcus aureus (VRSA) is one of the most important causes of antimicrobial resistance. VRSA possesses various regulatory factors to form and sustain biofilm in biotic or abiotic conditions. Among them, ureolytic activity is an important factor in the stabilization of biofilms by neutralizing the acidic environment
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Estrogen Sulfotransferase SULT1E1 Expression Levels and Regulated Factors in Malignant Tumours Protein Pept. Lett. (IF 1.6) Pub Date : 2023-09-15 Rui Wang, Xia Li, Yangyang Li, Mengjie Zhao, Lida Mi, Weiwei Chen, Jianxiang Song
Estrogen plays a key role in the development and progression of many malignant tumours, and the regulation of estrogen levels involves several metabolic pathways. Among these pathways, estrogen sulfotransferase (SULT1E1) is the enzyme with the most affinity for estrogen and is primarily responsible for catalysing the metabolic reaction of estrogen sulphation. Relevant studies have shown significant
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Biochemical Aspects of the Spiral Grain Formation in Scots Pine (Pinus Sylvestris L.) Wood. Some Differences and Similarities with Biochemical Indicators of Abnormal Xylogenesis in Karelian Birch (Betula Pendula Roth Var. Carelica (Mercl.) Hämet-Ahti) Protein Pept. Lett. (IF 1.6) Pub Date : 2023-09-15 Kseniya Mihajlovna Nikerova, Natalia Alekseevna Galibina, Sergey Mihajlovich Sinkevich, Irina Nikolaevna Sofronova, Marina Nikolaevna Borodina, Yuliya Leonidovna Moshchenskaya, Tatiana Vladimirovna Tarelkina, Anna Vladimirovna Klimova
Background: AOS enzymes can be biochemical indicators of abnormal xylogenesis in Scots pine, and this mechanism has similar features with the metabolic base of abnormal xylogenesis in Karelian birch. Objective: AOS enzymes’ activity in 150-300-year-old Pinus sylvestris L. wood with straight-- grained wood and right-twisted spiral-grained wood, expressed in varying degrees (5-20 angle), grew in three
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Comprehensive Analysis of lncRNA and mRNA Expression Profile of Macrophage RAW264.7 Stimulated by Antimicrobial Peptide BSN-37 Protein Pept. Lett. (IF 1.6) Pub Date : 2023-09-14 Ting Qin, Mingcheng Liu, Yanhe LV, Airong Zheng, Lei Wang, Yundi Wu, Oksana Kasianenko, Xiaobing Wei, Zhanwei Teng, Xiaojing Xia, Jianhe Hu
Background: BSN-37, a novel antimicrobial peptide (AMP) containing 37 amino acid residues isolated from the bovine spleen, has not only antibacterial activity but also immunomodulatory activity. Recent evidence shows that long non-coding RNAs (lncRNAs) play an important role in regulating the activation and function of immune cells. The purpose of this experiment was to investigate the lncRNA and mRNA
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Understanding the Molecular Basis for Enhanced Glutenase Activity of Actinidin using Structural Bioinformatics Protein Pept. Lett. (IF 1.6) Pub Date : 2023-09-04 Shivangi Puja, Shreya Seth, Rachna Hora, Satinder Kaur, Prakash Chandra Mishra
Background: Management of gluten intolerance is currently possible only by consumption of a gluten-free diet (GFD) for a lifetime. The scientific community has been searching for alternatives to GFD, like the inclusion of natural proteases with meals or pre-treatment of gluten-containing foods with glutenases. Actinidin from kiwifruit has shown considerable promise in digesting immunogenic gliadin
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Binding Affinity Studies of Nicotinamide N-methyltransferase and Ligands by Saturation Transfer Difference NMR Protein Pept. Lett. (IF 1.6) Pub Date : 2023-08-31 Tingting Fang, Jianyu Zhang
Introduction: Nicotinamide N-methyltransferase (NNMT) catalyzes the N-methylation of nicotinamide with S-adenosine-L-methionine (SAM) as the methyl donor. Abnormal expression of NNMT is associated with many diseases (such as multiple cancers and metabolic and liver diseases), making NNMT a potential therapeutic target. Limited studies concerning the enzymesubstrate/ inhibitor interactions could be
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The Emerging Roles of circRNAs in Papillary Thyroid Carcinoma: Molecular Mechanisms and Biomarker Potential Protein Pept. Lett. (IF 1.6) Pub Date : 2023-08-29 Haihan Ye, Xiaoyang Sun, Qianyun Ding, Enyu Yang, Shuo Zhao, Xiaowei Fan, Meiyu Fang, Xianfeng Ding
Papillary thyroid carcinoma (PTC) is a common endocrine malignant tumor. The incidence of PTC has increased in the past decades and presents a younger trend. Accumulating evidence indicates that circular RNAs (circRNAs), featured with non-linear, closed-loop structures, play pivotal roles in tumorigenesis and regulate cell biological processes, such as proliferation, migration, and invasion, by acting
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β Pore-forming Protein-based Evolutionary Divergence of Gnathostomata from Agnatha Protein Pept. Lett. (IF 1.6) Pub Date : 2023-08-17 Bhupendra Kumar, Mohd Kashif, Ahad Amer Alsaiari, Mohammad Imran Khan, Abul Kalam, Abrar Ahmad, Rayees Ahmad Lone, Mazen Almehmadi, Shivanand Suresh Dudhagi, Mazin A Zamzami
Introduction: The first vertebrates were jawless fish, or Agnatha, whose evolution diverged into jawed fish, or Gnathostomes, around 550 million years ago. Methods: In this study, we investigated β PFT proteins' evolutionary divergence of lamprey immune protein from Agnatha, reportedly possessing anti-cancer activity, into Dln1 protein from Gnathostomes. Both proteins showed structural and functional
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SNHG3/miR-330-5p/HSD11B1 Alleviates Myocardial Ischemia-reperfusion Injury by Regulating the ERK/p38 Signaling Pathway Protein Pept. Lett. (IF 1.6) Pub Date : 2023-08-04 Xiaochuan Bai, Jie Zhang, Heyun Yang, Keqiang Linghu, Min Xu
Background: Studies have found that microRNAs (miRNAs) participate in the pathogenesis of myocardial ischemia-reperfusion injury (MIRI). miR-330-5p alleviated cerebral IR injury and regulated myocardial damage. However, the mechanism of the effect of miR-330-5p on MIRI needs to be further studied. Objective: The study aimed to explore the role and mechanism of miR-330-5p in MIRI. Methods: The oxygen-glucose
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Effects of Conjugation of Ferrocene and Gallic Acid On desCys11/Lys12/Lys13-(p-BthTX-I)2K Peptide: Structure, Permeabilization and Antibacterial Activity Protein Pept. Lett. (IF 1.6) Pub Date : 2023-08-04 Marina Rodrigues Pereira, Vanessa dos Santos Rodrigues, Warlley Campos de Oliveira, Cristiane Duque, Benise Ferreira da Silva, Norival Alves Santos-Filho, Victor Alves Carneiro, Esteban Nicolás Lorenzón, Eduardo Maffud Cilli
Background: Antimicrobial resistance is an emerging global health challenge that has led researchers to study alternatives to conventional antibiotics. A promising alternative is antimicrobial peptides (AMPs), produced as the first line of defense by almost all living organisms. To improve its biological activity, the conjugation of AMPs is a promising approach. Objective: In this study, we evaluated
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A Comprehensive Review on Inorganic Nanoparticles as Effective Modulators of Amyloidogenesis Protein Pept. Lett. (IF 1.6) Pub Date : 2023-07-20 Debashmita Chakraborty, Aniket Mukherjee, Nandini Sarkar
Many degenerative disorders have started to develop as a result of the deposition of insoluble protein fibrillar clumps known as amyloid. This deposition mostly limits normal cellular function and signaling. This build-up of amyloid in vivo results in a variety of illnesses in the body, including type 2 diabetes, several neurodegenerative diseases (such as Alzheimer's disease and spongiform encephalopathy)
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Recent Advances in Bioactive Peptides as Functional Food for Health Promotions and Medicinal Applications Protein Pept. Lett. (IF 1.6) Pub Date : 2023-07-19 P.J. Sreelekshmi, V. Devika, S. Aiswarya Lakshmi, R. Jeevan Sankar, K. Ramanunni, Pranav B. Nair, Sandhya Sadanandan
Bioactive peptides obtained from natural resources are useful due to their ability to prevent the risk of dreadful conditions such as hypertension, cancers, obesity and cardiovascular diseases. Proteins from food, plants, animals and dairy products are chemically or enzymatically hydrolyzed or fermented in the presence of microbes to produce bioactive peptides. Bioactive peptides are antioxidant, antihypertensive