-
Let it glow: genetically encoded fluorescent reporters in Plasmodium Malaria J. (IF 3.0) Pub Date : 2024-04-20 Pia J. Thiele, Raquel Mela-Lopez, Stéphanie A. Blandin, Dennis Klug
The use of fluorescent proteins (FPs) in Plasmodium parasites has been key to understand the biology of this obligate intracellular protozoon. FPs like the green fluorescent protein (GFP) enabled to explore protein localization, promoter activity as well as dynamic processes like protein export and endocytosis. Furthermore, FP biosensors have provided detailed information on physiological parameters
-
Willingness to accept and participate in a Microsporidia MB-based mosquito release strategy: a community-based rapid assessment in western Kenya Malaria J. (IF 3.0) Pub Date : 2024-04-20 Tullu Bukhari, Joseph Gichuhi, Oscar Mbare, Victoria A. Ochwal, Ulrike Fillinger, Jeremy K. Herren
Microsporidia MB, an endosymbiont naturally found in Anopheles mosquitoes inhibits transmission of Plasmodium and is a promising candidate for a transmission-blocking strategy that may involve mosquito release. A rapid assessment was carried out to develop insight into sociodemographic factors, public health concerns, and malaria awareness, management, and prevention practices with the willingness
-
Effect of spatiotemporal variables on abundance, biting activity and parity of Nyssorhynchus darlingi (Diptera: Culicidae) in peri-Iquitos, Peru Malaria J. (IF 3.0) Pub Date : 2024-04-19 Sara A. Bickersmith, Marlon P. Saavedra, Catharine Prussing, Rachel E. Lange, Juliana A. Morales, Freddy Alava, Joseph M. Vinetz, Dionicia Gamboa, Marta Moreno, Jan E. Conn
In malaria endemic regions of the Peruvian Amazon, rainfall together with river level and breeding site availability drive fluctuating vector mosquito abundance and human malaria cases, leading to temporal heterogeneity. The main variables influencing spatial transmission include location of communities, mosquito behaviour, land use/land cover, and human ecology/behaviour. The main objective was to
-
Ageing of Plasmodium falciparum malaria sporozoites alters their motility, infectivity and reduces immune activation in vitro Malaria J. (IF 3.0) Pub Date : 2024-04-19 Roos van Schuijlenburg, Samaneh Azargoshasb, Clarize M. de Korne, Jeroen C. Sijtsma, Sascha Bezemer, Alwin J. van der Ham, Els Baalbergen, Fiona Geurten, Laura M. de Bes-Roeleveld, Severine C. Chevalley-Maurel, Matthias N. van Oosterom, Fijs W. B. van Leeuwen, Blandine Franke-Fayard, Meta Roestenberg
Sporozoites (SPZ), the infective form of Plasmodium falciparum malaria, can be inoculated into the human host skin by Anopheline mosquitoes. These SPZ migrate at approximately 1 µm/s to find a blood vessel and travel to the liver where they infect hepatocytes and multiply. In the skin they are still low in number (50–100 SPZ) and vulnerable to immune attack by antibodies and skin macrophages. This
-
Aspecific binding of anti-NK1.1 antibodies on myeloid cells in an experimental model for malaria-associated acute respiratory distress syndrome Malaria J. (IF 3.0) Pub Date : 2024-04-18 Emilie Pollenus, Fran Prenen, Hendrik Possemiers, Sofie Knoops, Tania Mitera, Jochen Lamote, Amber De Visscher, Leen Vandermosten, Thao-Thy Pham, Patrick Matthys, Philippe E. Van den Steen
Conventional natural killer (cNK) cells play an important role in the innate immune response by directly killing infected and malignant cells and by producing pro- and anti-inflammatory cytokines. Studies on their role in malaria and its complications have resulted in conflicting results. Using the commonly used anti-NK1.1 depletion antibodies (PK136) in an in-house optimized experimental model for
-
Factors associated with the risk of malaria among children: analysis of 2021 Nigeria Malaria Indicator Survey Malaria J. (IF 3.0) Pub Date : 2024-04-17 Isaac Isiko, Simon Nyegenye, Daniel Kiprotich Bett, Jackson Micheal Asingwire, Lenz Nwachinemere Okoro, Nana Awaya Emeribe, Catherine Chepkoskei Koech, Ovye Ahgu, Naya Gadzama Bulus, Kelly Taremwa, Aaron Mwesigwa
Malaria remains a burden globally, with the African region accounting for 94% of the overall disease burden and deaths in 2019. It is the major cause of morbidity and mortality among children in Nigeria. Though different environmental factors have been assessed to influence the distribution and transmission of malaria vectors, there is a shortage of information on how they may influence malaria transmission
-
Widespread pfhrp2/3 deletions and HRP2-based false-negative results in southern Ethiopia Malaria J. (IF 3.0) Pub Date : 2024-04-17 Bacha Mekonen, Sisay Dugassa, Sindew Mekasha Feleke, Boja Dufera, Bedasa Gidisa, Aderaw Adamu, Aynalem Mandefro, Geremew Tasew, Lemu Golassa
Rapid diagnostic tests (RDTs) play a significant role in expanding case management in peripheral healthcare systems. Histidine-rich protein-2 (HRP2) antigen detection RDTs are predominantly used to diagnose Plasmodium falciparum infection. However, the evolution and spread of P. falciparum parasite strains with deleted hrp2/3 genes, causing false-negative results, have been reported. This study assessed
-
Mapping malaria vectors and insecticide resistance in a high-endemic district of Haryana, India: implications for vector control strategies Malaria J. (IF 3.0) Pub Date : 2024-04-17 Gaurav Kumar, Sanjeev Gupta, Jaspreet Kaur, Shweta Pasi, Rajendra Baharia, Ajeet Kumar Mohanty, Pawan Goel, Amit Sharma, Manju Rahi
Achieving effective control and elimination of malaria in endemic regions necessitates a comprehensive understanding of local mosquito species responsible for malaria transmission and their susceptibility to insecticides. The study was conducted in the highly malaria prone Ujina Primary Health Center of Nuh (Mewat) district of Haryana state of India. Monthly entomological surveys were carried out for
-
Superior protection in a relapsing Plasmodium cynomolgi rhesus macaque model by a chemoprophylaxis with sporozoite immunization regimen with atovaquone-proguanil followed by primaquine Malaria J. (IF 3.0) Pub Date : 2024-04-17 Kosol Yongvanitchit, Utaiwan Kum-Arb, Amporn Limsalakpetch, Rawiwan Im-Erbsin, Ratawan Ubalee, Michele D. Spring, Brian A. Vesely, Norman Waters, Sathit Pichyangkul
To gain a deeper understanding of protective immunity against relapsing malaria, this study examined sporozoite-specific T cell responses induced by a chemoprophylaxis with sporozoite (CPS) immunization in a relapsing Plasmodium cynomolgi rhesus macaque model. The animals received three CPS immunizations with P. cynomolgi sporozoites, administered by mosquito bite, while under two anti-malarial drug
-
Factors associated with malaria vaccine uptake in Nsanje district, Malawi Malaria J. (IF 3.0) Pub Date : 2024-04-17 Atusaye J. Simbeye, Save Kumwenda, Lauren M. Cohee, Dickens Omondi, Peninah K. Masibo, Hesborn Wao, Shehu S. Awandu
Malaria remains a significant global health burden affecting millions of people, children under 5 years and pregnant women being most vulnerable. In 2019, the World Health Organization (WHO) endorsed the introduction of RTS,S/AS01 malaria vaccine as Phase IV implementation evaluation in three countries: Malawi, Kenya and Ghana. Acceptability and factors influencing vaccination coverage in implementing
-
Detection of P. malariae using a new rapid isothermal amplification lateral flow assay Malaria J. (IF 3.0) Pub Date : 2024-04-12 Ashenafi Assefa, Kevin Wamae, Christopher M. Hennelly, Billy Ngasala, Meredith Muller, Albert Kalonji, Fernandine Phanzu, Clark H. Cunningham, Jessica T. Lin, Jonathan B. Parr
While Plasmodium falciparum and Plasmodium vivax cause the majority of malaria cases and deaths, infection by Plasmodium malariae and other Plasmodium species also causes morbidity and mortality. Current understanding of these infections is limited in part by existing point-of-care diagnostics that fail to differentiate them and have poor sensitivity for low-density infections. Accurate diagnosis currently
-
The effect of an anti-malarial herbal remedy, Maytenus senegalensis, on electrocardiograms of healthy Tanzanian volunteers Malaria J. (IF 3.0) Pub Date : 2024-04-12 Kamaka R. Kassimu, Ali M. Ali, Justin J. Omolo, Abel Mdemu, Francis Machumi, Billy Ngasala
The emergence of resistance to artemisinin-based combination therapy necessitates the search for new, more potent antiplasmodial compounds, including herbal remedies. The whole extract of Maytenus senegalensis has been scientifically investigated for potential biological activities both in vitro and in vivo, demonstrating strong antimalarial activity. However, there is a lack of data on the electrocardiographic
-
Modelling spatiotemporal variation in under-five malaria risk in Ghana in 2016–2021 Malaria J. (IF 3.0) Pub Date : 2024-04-09 Justice Moses K. Aheto, Lynette J. Menezes, Wisdom Takramah, Liwang Cui
Ghana is among the top 10 highest malaria burden countries, with about 20,000 children dying annually, 25% of which were under five years. This study aimed to produce interactive web-based disease spatial maps and identify the high-burden malaria districts in Ghana. The study used 2016–2021 data extracted from the routine health service nationally representative and comprehensive District Health Information
-
Efficacy and safety of artemether-lumefantrine for the treatment of uncomplicated falciparum malaria in mainland Tanzania, 2019 Malaria J. (IF 3.0) Pub Date : 2024-04-09 Billy E. Ngasala, Mercy G. Chiduo, Bruno P. Mmbando, Filbert T. Francis, Samwel Bushukatale, Twilumba Makene, Celine I. Mandara, Deus S. Ishengoma, Erasmus Kamugisha, Maimuna Ahmed, Muhidin K. Mahende, Reginald A. Kavishe, Florida Muro, Fabrizio Molteni, Erik Reaves, Chonge Kitojo, George Greer, Ssanyu Nyinondi, Bilal Kabula, Shabbir Lalji, Frank Chacky, Ritha J. Njau, Marian Warsame, Ally Mohamed
Artemisinin-based combination therapy (ACT) has been a major contributor to the substantial reductions in global malaria morbidity and mortality over the last decade. In Tanzania, artemether-lumefantrine (AL) was introduced as the first-line treatment for uncomplicated Plasmodium falciparum malaria in 2006. The World Health Organization (WHO) recommends regular assessment and monitoring of the efficacy
-
The effect of physical barriers under a raised house on mosquito entry: an experimental study in rural Gambia Malaria J. (IF 3.0) Pub Date : 2024-04-08 Majo Carrasco-Tenezaca, Musa Jawara, John Bradley, Umberto D’Alessandro, David Jeffries, Jakob B. Knudsen, Steve W. Lindsay
Anopheles gambiae, the major malaria mosquito in sub-Saharan Africa, feed largely indoors at night. Raising a house off the ground with no barriers underneath reduces mosquito-house entry. This experiment tested whether walling off the space under an elevated hut affects mosquito-hut entry. Four inhabited experimental huts, each of which could be moved up and down, were used in rural Gambia. Nightly
-
A formative cross-sectional study to assess caregiver’s health-seeking behaviour and knowledge surrounding malaria, and understand the burden of malaria among children under-five in conflict-affected communities of Cameroon Malaria J. (IF 3.0) Pub Date : 2024-04-08 Elvis Asangbeng Tanue, Lundi-Anne Omam, Glennis T. Ayuk, Bibiche Modjenpa Noukeme, Alain Metuge, Isabelle Nganmou, Margaret Besem Ebob, Laura Donovan, Ann-Sophie Stratil, Helen Counihan, Claude Ngwayu Nkfusai, Helen Hawkings, Blanka Homolova, Elizabeth Berryman, Maxwell Kolawole, Yakouba Zoungrana, Dorothy Achu, Samuel Wanji, Esther Njomo Omam
Malaria remains a major global health problem often worsened by political instability and armed conflict. The purpose of the study was to explore community knowledge, attitudes and practices on malaria prevention, and to understand the burden of malaria and health-seeking behaviours of caregivers of children under-five in conflict-affected communities of the South West and Littoral Regions of Cameroon
-
Correction: Malaria elimination and the need for intensive inter-country cooperation: a critical evaluation of regional technical co-operation in Southern Africa Malaria J. (IF 3.0) Pub Date : 2024-04-08 Chadwick H. Sikaala, Bongani Dlamini, Alphart Lungu, Phelele Fakudze, Mukosha Chisenga, Chishala Lukwesa Siame, Nyasha Mwendera, Dumisani Shaba, John M. Chimumbwa, Immo Kleinschmidt
Correction: Malaria Journal (2024) 23:62 https://doi.org/10.1186/s12936-024-04891-5 Following publication of the article [1], it came to the journal's attention that there was an error in Fig. 3: the image of the figure (the figure apart from the caption) had been erroneously duplicated and showed twice as a result. The figure has since been corrected in the article. The publisher thanks you for reading
-
Plasmodium falciparum genetic diversity and multiplicity of infection based on msp-1, msp-2, glurp and microsatellite genetic markers in sub-Saharan Africa: a systematic review and meta-analysis Malaria J. (IF 3.0) Pub Date : 2024-04-08 Alex Mwesigwa, Moses Ocan, Benson Musinguzi, Rachel Wangi Nante, Joaniter I. Nankabirwa, Steven M. Kiwuwa, Alison Annet Kinengyere, Barbara Castelnuovo, Charles Karamagi, Ekwaro A. Obuku, Samuel L. Nsobya, Sam M. Mbulaiteye, Pauline Byakika-Kibwika
In sub-Saharan Africa (SSA), Plasmodium falciparum causes most of the malaria cases. Despite its crucial roles in disease severity and drug resistance, comprehensive data on Plasmodium falciparum genetic diversity and multiplicity of infection (MOI) are sparse in SSA. This study summarizes available information on genetic diversity and MOI, focusing on key markers (msp-1, msp-2, glurp, and microsatellites)
-
Genotyping Plasmodium falciparum gametocytes using amplicon deep sequencing Malaria J. (IF 3.0) Pub Date : 2024-04-06 Jimmy Vareta, Natalie A. Horstman, Matthew Adams, Karl B. Seydel, Robert S. McCann, Lauren M. Cohee, Miriam K. Laufer, Shannon Takala-Harrison
Understanding the dynamics of gametocyte production in polyclonal Plasmodium falciparum infections requires a genotyping method that detects distinct gametocyte clones and estimates their relative frequencies. Here, a marker was identified and evaluated to genotype P. falciparum mature gametocytes using amplicon deep sequencing. A data set of polymorphic regions of the P. falciparum genome was mined
-
Efficacy and safety of artemether-lumefantrine for the treatment of uncomplicated falciparum malaria in mainland Tanzania, 2018 Malaria J. (IF 3.0) Pub Date : 2024-04-06 Billy Ngasala, Mercy G. Chiduo, Samwel Bushukatale, Bruno P. Mmbando, Twilumba Makene, Erasmus Kamugisha, Maimuna Ahmed, Celine I. Mandara, Filbert Francis, Muhidin K. Mahende, Reginald A. Kavishe, Florida Muro, Deus S. Ishengoma, Renata Mandike, Fabrizio Molteni, Frank Chacky, Chonge Kitojo, George Greer, Dunstan Bishanga, Jasmine Chadewa, Ritha Njau, Marian Warsame, Bilali Kabula, Ssanyu S. Nyinondi
The use of artemisinin-based combination therapy (ACT) is recommended by the World Health Organization for the treatment of uncomplicated falciparum malaria. Artemether-lumefantrine (AL) is the most widely adopted first-line ACT for uncomplicated malaria in sub-Saharan Africa (SSA), including mainland Tanzania, where it was introduced in December 2006. The WHO recommends regular assessment to monitor
-
Low long-lasting insecticidal net use in malaria elimination areas in Southern Ethiopia: results from community based cross-sectional study Malaria J. (IF 3.0) Pub Date : 2024-04-04 Misganu Endriyas, Mekidim Kassa, Yilma Chisha, Emebet Mekonnen, Tebeje Misganaw, Eskindir Loha, Ayalew Astatkie
Despite remarkable progress in malaria burden reduction, malaria continues to be a major public health problem globally. Ethiopia has been distributing long-lasting insecticidal nets (LLINs) for free and nationwide distribution was completed in 2016. However, evidence suggests that the utilization of LLINs varies from setting to setting and from time to time due to different factors, and up-to-date
-
A case of severe Plasmodium ovale malaria with acute respiratory distress syndrome and splenic infarction in a male traveller presenting in Italy Malaria J. (IF 3.0) Pub Date : 2024-04-04 Maria Virginia Tomassi, Alessandra D’Abramo, Serena Vita, Angela Corpolongo, Antonella Vulcano, Tommaso Ascoli Bartoli, Barbara Bartolini, Francesca Faraglia, Emanuele Nicastri
Plasmodium ovale malaria is usually considered a tropical infectious disease associated with low morbidity and mortality. However, severe disease and death have previously been reported. A case of severe P. ovale malaria in a healthy Caucasian man with a triangle splenic infarction and clinical progression towards Acute Respiratory Distress Syndrome was reported despite a rapid response to oral chloroquine
-
Efficacy of artemether-lumefantrine and dihydroartemisinin-piperaquine and prevalence of molecular markers of anti-malarial drug resistance in children in Togo in 2021 Malaria J. (IF 3.0) Pub Date : 2024-04-03 Ameyo Monique Dorkenoo, Marian Warsame, Essoham Ataba, Manani Hemou, Kossi Yakpa, Efoe Sossou, M’badi Mitigmsagou, Carmel Diwaba Teou, Emmanuelle Caspar, Laurence Ma, Koffi Edem Djadou, Tinah Atcha-Oubou, Charlotte Rasmussen, Didier Menard
Artemether-lumefantrine (AL) and dihydroartemisinin-piperaquine (DP) are the currently recommended first- and second-line therapies for uncomplicated Plasmodium falciparum infections in Togo. This study assessed the efficacy of these combinations, the proportion of Day3-positive patients (D3 +), the proportion of molecular markers associated with P. falciparum resistance to anti-malarial drugs, and
-
Receipt of seasonal malaria chemoprevention by age-ineligible children and associated factors in nine implementation states in Nigeria Malaria J. (IF 3.0) Pub Date : 2024-03-30 Taiwo Ibinaiye, Kunle Rotimi, Ayodeji Balogun, Adaeze Aidenagbon, Chibuzo Oguoma, Christian Rassi, Kevin Baker, Olusola Oresanya, Chuks Nnaji
As part of implementation quality standards, community distributors are expected to ensure that only age-eligible children (aged 3–59 months) receive seasonal malaria chemoprevention (SMC) medicines during monthly campaigns. There is uncertainty about the extent to which SMC medicines are administered to ineligible children. This study aimed to assess the magnitude of this occurrence, while exploring
-
Efficacy of artesunate-amodiaquine for treatment of uncomplicated Plasmodium falciparum malaria in mainland Tanzania Malaria J. (IF 3.0) Pub Date : 2024-03-29 Billy Ngasala, Samwel Bushukatale, Mercy Chiduo, Twilumba Makene, Lilian Mkony, Ally Mohamed, Fablizio Molteni, Frank Chacky, Ritha J. A. Njau, Richard Mwaiswelo
Diversification of artemisinin-based combination therapy (ACT) is suggested as one of the strategies that can be used to contain artemisinin resistance. Artesunate-amodiaquine (ASAQ) is one of the artemisinin-based combinations that can be used in the diversification strategy as an alternative first-line treatment for uncomplicated malaria in mainland Tanzania. There is however limited data on the
-
Implementation of post-discharge malaria chemoprevention (PDMC) in Benin, Kenya, Malawi, and Uganda: stakeholder engagement meeting report Malaria J. (IF 3.0) Pub Date : 2024-03-27 Jenny Hill
A Stakeholder engagement meeting on the implementation of post-discharge malaria chemoprevention (PDMC) in Benin, Kenya, Malawi, and Uganda was held in Nairobi, Kenya, on 27 September 2023. Representatives from the respective National Malaria Control Programmes, the World Health Organization (WHO) Geneva, Africa Regional and Kenya offices, research partners, non-governmental organizations, and the
-
A systematic review: is Anopheles vagus a species complex? Malaria J. (IF 3.0) Pub Date : 2024-03-27 Dalilah Dalilah, Din Syafruddin, Irsan Saleh, Ahmad Ghiffari, Leo Vernadesly, Lepa Syahrani, Irdayanti Irdayanti, Chairil Anwar
Anopheles vagus (subgenus Cellia) has been identified as a vector for malaria, filariasis, and Japanese encephalitis in Asia. Sporozoites of Plasmodium falciparum and Plasmodium vivax have been found in this zoophilic mosquito in Asia and Indonesia. This study systematically reviews publications regarding An. vagus species, variation, bio-ecology, and malaria transmission in various localities in Asia
-
An evaluation of LLIN physical integrity and population attitudes towards net use, care and handling during the Magude project in southern Mozambique Malaria J. (IF 3.0) Pub Date : 2024-03-27 Celso Alafo, Lucia Fernandez Montoya, Helena Martí-Soler, Mara Máquina, Arlindo Malheia, Charfudin Sacoor, Ana Paula Abílio, Dulcisaria Marrenjo, Nelson Cuamba, Beatriz Galatas, Pedro Aide, Francisco Saúte, Krijn P. Paaijmans
The Magude Project assessed the feasibility of eliminating malaria in Magude district, a low transmission setting in southern Mozambique, using a package of interventions, including long-lasting insecticidal nets (LLINs). As the efficacy of LLINs depends in part on their physical integrity, this metric was quantified for Olyset® Nets post mass-distribution, in addition to net use, care and handling
-
Rapid assessment of the blood-feeding histories of wild-caught malaria mosquitoes using mid-infrared spectroscopy and machine learning Malaria J. (IF 3.0) Pub Date : 2024-03-26 Emmanuel P. Mwanga, Idrisa S. Mchola, Faraja E. Makala, Issa H. Mshani, Doreen J. Siria, Sophia H. Mwinyi, Said Abbasi, Godian Seleman, Jacqueline N. Mgaya, Mario González Jiménez, Klaas Wynne, Maggy T. Sikulu-Lord, Prashanth Selvaraj, Fredros O. Okumu, Francesco Baldini, Simon A. Babayan
The degree to which Anopheles mosquitoes prefer biting humans over other vertebrate hosts, i.e. the human blood index (HBI), is a crucial parameter for assessing malaria transmission risk. However, existing techniques for identifying mosquito blood meals are demanding in terms of time and effort, involve costly reagents, and are prone to inaccuracies due to factors such as cross-reactivity with other
-
The effect of COVID-19 on malaria cases in Zambia: a mixed effect multilevel analysis Malaria J. (IF 3.0) Pub Date : 2024-03-22 Mutale Sampa, Ronald Fisa, Chilombo Mukuma, Mercy Mwanza, Busiku Hamainza, Patrick Musonda
The burden of Malaria in Zambia remains a challenge, with the entire population at risk of contracting this infectious disease. Despite concerted efforts by African countries, including Zambia, to implement malaria policies and strategies aimed at reducing case incidence, the region faces significant hurdles, especially with emerging pandemics such as COVID-19. The efforts to control malaria were impacted
-
Cerebral malaria presenting as nonconvulsive status epilepticus: a case report Malaria J. (IF 3.0) Pub Date : 2024-03-18 Xingguo Wu, Ningxiang Qin, Fahang Yi, Jing Wang, Xia Yan, Liang Wang
Malaria is an infectious malady caused by Plasmodium parasites, cerebral malaria standing out as one of its most severe complications. Clinical manifestation include elevated body temperature, loss of consciousness, and seizures. However, reports of cerebral malaria presenting as nonconvulsive status epilepticus are extremely rare. The case presented involves psychiatric symptoms, with the electroencephalogram
-
Community and health worker perspectives on malaria in Meghalaya, India: covering the last mile of elimination by 2030 Malaria J. (IF 3.0) Pub Date : 2024-03-18 Carinthia B. Nengnong, Mattimi Passah, Mark L. Wilson, Elisa Bellotti, Anne Kessler, Bibha R. Marak, Jane M. Carlton, Rajiv Sarkar, Sandra Albert
Malaria remains a public health problem in regions of Northeastern India because of favourable bio-geographic transmission conditions, poor access to routine healthcare, and inadequate infrastructure for public health and disease prevention. This study was undertaken to better understand community members’ and health workers’ perceptions of malaria, as well as their knowledge, attitudes, and prevention
-
Malaria infection among adults residing in a highly endemic region from the Democratic Republic of the Congo Malaria J. (IF 3.0) Pub Date : 2024-03-18 Nadine Kalenda Kayiba, Yuko Nitahara, Evariste Tshibangu-Kabamba, Denis Kalambayi Mbuyi, Augustin Kabongo-Tshibaka, Nestor Tshituka Kalala, Barthélemy Mukenga Tshiebue, Katherine-Sofia Candray-Medina, Natsuko Kaku, Yu Nakagama, Niko Speybroeck, Dieudonné Ngoyi Mumba, Ghislain Tumba Disashi, Akira Kaneko, Yasutoshi Kido
Adults infected with Plasmodium spp. in endemic areas need to be re-evaluated in light of global malaria elimination goals. They potentially undermine malaria interventions but remain an overlooked aspect of public health strategies. This study aimed to estimate the prevalence of Plasmodium spp. infections, to identify underlying parasite species, and to assess predicting factors among adults residing
-
Comparison of different trapping methods to collect malaria vectors indoors and outdoors in western Kenya Malaria J. (IF 3.0) Pub Date : 2024-03-16 Jackline Kosgei, John E. Gimnig, Vincent Moshi, Seline Omondi, Daniel P. McDermott, Martin J. Donnelly, Collins Ouma, Bernard Abong’o, Eric Ochomo
Vector surveillance is among the World Health Organization global vector control response (2017–2030) pillars. Human landing catches are a gold standard but difficult to implement and potentially expose collectors to malaria infection. Other methods like light traps, pyrethrum spray catches and aspiration are less expensive and less risky to collectors. Three mosquito sampling methods (UV light traps
-
Cost effectiveness of malaria vector control activities in Sudan Malaria J. (IF 3.0) Pub Date : 2024-03-15 Sami M. HasapAla, Rasha S. Azrag, Osama M. Awad
Malaria vector control activities in Sudan rely largely on Long-Lasting Insecticidal Nets (LLINs), Indoor Residual Spray (IRS) and Larval Source Management (LSM). The present study attempted to determine cost effectiveness of inputs and operations of vector control interventions applied in different environmental settings in central and eastern Sudan, as well as their impact. The inputs utilized and
-
Microsatellites reveal high polymorphism and high potential for use in anti-malarial efficacy studies in areas with different transmission intensities in mainland Tanzania Malaria J. (IF 3.0) Pub Date : 2024-03-15 Deus S. Ishengoma, Celine I. Mandara, Rashid A. Madebe, Marian Warsame, Billy Ngasala, Abdunoor M. Kabanywanyi, Muhidin K. Mahende, Erasmus Kamugisha, Reginald A. Kavishe, Florida Muro, Renata Mandike, Sigsbert Mkude, Frank Chacky, Ritha Njau, Troy Martin, Ally Mohamed, Jeffrey A. Bailey, Abebe A. Fola
Tanzania is currently implementing therapeutic efficacy studies (TES) in areas of varying malaria transmission intensities as per the World Health Organization (WHO) recommendations. In TES, distinguishing reinfection from recrudescence is critical for the determination of anti-malarial efficacy. Recently, the WHO recommended genotyping polymorphic coding genes, merozoite surface proteins 1 and 2 (msp1
-
Application of advanced very high-resolution radiometer (AVHRR)-based vegetation health indices for modelling and predicting malaria in Northern Benin, West Africa Malaria J. (IF 3.0) Pub Date : 2024-03-15 Gouvidé Jean Gbaguidi, Mouhamed Idrissou, Nikita Topanou, Walter Leal Filho, Guillaume K. Ketoh
Vegetation health (VH) is a powerful characteristic for forecasting malaria incidence in regions where the disease is prevalent. This study aims to determine how vegetation health affects the prevalence of malaria and create seasonal weather forecasts using NOAA/AVHRR environmental satellite data that can be substituted for malaria epidemic forecasts. Weekly advanced very high-resolution radiometer
-
Insecticide susceptibility status of Anopheles gambiae mosquitoes and the effect of pre-exposure to a piperonyl butoxide (PBO) synergist on resistance to deltamethrin in northern Namibia Malaria J. (IF 3.0) Pub Date : 2024-03-14 Rosalia N. Joseph, Tabeth Mwema, Seth J. Eiseb, Deodatus V. Maliti, Munyaradzi Tambo, Iitula Iitula, Lydia Eloff, Ophilia Lukubwe, Cara Smith-Gueye, Élodie A. Vajda, Allison Tatarsky, Stark T. Katokele, Petrina N. Uusiku, Dennis Walusimbi, Sheila B. Ogoma, Davis R. Mumbengegwi, Neil F. Lobo
Pyrethroid-based indoor residual spraying (IRS) and long-lasting insecticidal nets (LLINs) have been employed as key vector control measures against malaria in Namibia. However, pyrethroid resistance in Anopheles mosquitoes may compromise the efficacy of these interventions. To address this challenge, the World Health Organization (WHO) recommends the use of piperonyl butoxide (PBO) LLINs in areas
-
Epidemiology of Plasmodium vivax in Duffy negatives and Duffy positives from community and health centre collections in Ethiopia Malaria J. (IF 3.0) Pub Date : 2024-03-14 Lauren Bradley, Delenasaw Yewhalaw, Elizabeth Hemming-Schroeder, Brook Jeang, Ming-Chieh Lee, Endalew Zemene, Teshome Degefa, Eugenia Lo, Christopher King, James Kazura, Guiyun Yan
Malaria remains a significant cause of morbidity and mortality in Ethiopia with an estimated 3.8 million cases in 2021 and 61% of the population living in areas at risk of malaria transmission. Throughout the country Plasmodium vivax and Plasmodium falciparum are co-endemic, and Duffy expression is highly heterogeneous. The public health significance of Duffy negativity in relation to P. vivax malaria
-
Comparing malaria risk exposure in rural Cambodia population using GPS tracking and questionnaires Malaria J. (IF 3.0) Pub Date : 2024-03-12 Anaïs Pepey, Marc Souris, Saorin Kim, Thomas Obadia, Sophy Chy, Malen Ea, Sivkeng Ouk, Franck Remoue, Siv Sovannaroth, Ivo Mueller, Benoit Witkowski, Amélie Vantaux
The Great Mekong Subregion has attained a major decline in malaria cases and fatalities over the last years, but residual transmission hotspots remain, supposedly fueled by forest workers and migrant populations. This study aimed to: (i) characterize the fine-scale mobility of forest-goers and understand links between their daily movement patterns and malaria transmission, using parasites detection
-
Malaria transmission heterogeneity in different eco-epidemiological areas of western Kenya: a region-wide observational and risk classification study for adaptive intervention planning Malaria J. (IF 3.0) Pub Date : 2024-03-12 Guofa Zhou, John Githure, Ming-Chieh Lee, Daibin Zhong, Xiaoming Wang, Harrysone Atieli, Andrew K. Githeko, James Kazura, Guiyun Yan
Understanding of malaria ecology is a prerequisite for designing locally adapted control strategies in resource-limited settings. The aim of this study was to utilize the spatial heterogeneity in malaria transmission for the designing of adaptive interventions. Field collections of clinical malaria incidence, asymptomatic Plasmodium infection, and malaria vector data were conducted from 108 randomly
-
Factors affecting delays in seeking treatment among malaria patients during the pre-certification phase in China Malaria J. (IF 3.0) Pub Date : 2024-03-11 Lianyu Jia, Xiaoyu Chen, Zhanchun Feng, Shangfeng Tang, Da Feng
Delays in malaria treatment can not only lead to severe and even life-threatening complications, but also foster transmission, putting more people at risk of infection. This study aimed to investigate the factors influencing treatment delays among malaria patients and their health-seeking behaviour. The medical records of 494 patients diagnosed with malaria from 6 different malaria-endemic provinces
-
First report of natural infection of Anopheles gambiae s.s. and Anopheles coluzzii by Wolbachia and Microsporidia in Benin: a cross-sectional study Malaria J. (IF 3.0) Pub Date : 2024-03-11 Minassou Juvénal Ahouandjinou, Arthur Sovi, Aboubakar Sidick, Wilfried Sewadé, Come Zinsou Koukpo, Saïd Chitou, Linda Towakinou, Bruno Adjottin, Steve Hougbe, Filémon Tokponnon, Germain Gil Padonou, Martin Akogbéto, Louisa A. Messenger, Razaki A. Ossè
Recently, bacterial endosymbiont, including Wolbachia and Microsporidia were found to limit the infection of Anopheles mosquitoes with Plasmodium falciparum. This study aimed to investigate the natural presence of key transmission-blocking endosymbionts in Anopheles gambiae and Anopheles coluzzii in Southern Benin. The present study was conducted in seven communes (Cotonou, Porto-Novo, Aguégués, Ifangni
-
Trends of Plasmodium falciparum molecular markers associated with resistance to artemisinins and reduced susceptibility to lumefantrine in Mainland Tanzania from 2016 to 2021 Malaria J. (IF 3.0) Pub Date : 2024-03-09 Catherine Bakari, Celine I. Mandara, Rashid A. Madebe, Misago D. Seth, Billy Ngasala, Erasmus Kamugisha, Maimuna Ahmed, Filbert Francis, Samwel Bushukatale, Mercy Chiduo, Twilumba Makene, Abdunoor M. Kabanywanyi, Muhidin K. Mahende, Reginald A. Kavishe, Florida Muro, Sigsbert Mkude, Renata Mandike, Fabrizio Molteni, Frank Chacky, Dunstan R. Bishanga, Ritha J. A. Njau, Marian Warsame, Bilali Kabula
Therapeutic efficacy studies (TESs) and detection of molecular markers of drug resistance are recommended by the World Health Organization (WHO) to monitor the efficacy of artemisinin-based combination therapy (ACT). This study assessed the trends of molecular markers of artemisinin resistance and/or reduced susceptibility to lumefantrine using samples collected in TES conducted in Mainland Tanzania
-
RNAscope in situ hybridization reveals microvascular sequestration of Plasmodium relictum pSGS1 blood stages but absence of exo-erythrocytic dormant stages during latent infection of Serinus canaria Malaria J. (IF 3.0) Pub Date : 2024-03-08 Tanja Himmel, Josef Harl, Julia Matt, Nora Nedorost, Tatjana Iezhova, Mikas Ilgūnas, Gediminas Valkiūnas, Herbert Weissenböck
Birds chronically infected with avian malaria parasites often show relapses of parasitaemia after latent stages marked by absence of parasites in the peripheral circulation. These relapses are assumed to result from the activation of dormant exo-erythrocytic stages produced during secondary (post-erythrocytic) merogony of avian Plasmodium spp. Yet, there is no morphological proof of persistent or dormant
-
The impact of agrochemical pollutant mixtures on the selection of insecticide resistance in the malaria vector Anopheles gambiae: insights from experimental evolution and transcriptomics Malaria J. (IF 3.0) Pub Date : 2024-03-05 Christabelle G. Sadia, Jean-Marc Bonneville, Marius G. Zoh, Behi K. Fodjo, France-Paraudie A. Kouadio, Sebastien K. Oyou, Benjamin G. Koudou, Beatrice A. Adepo-Gourene, Stephane Reynaud, Jean-Philippe David, Chouaibou S. Mouahamadou
There are several indications that pesticides used in agriculture contribute to the emergence and spread of resistance of mosquitoes to vector control insecticides. However, the impact of such an indirect selection pressure has rarely been quantified and the molecular mechanisms involved are still poorly characterized. In this context, experimental selection with different agrochemical mixtures was
-
Evaluating the performance of Plasmodium falciparum genetic metrics for inferring National Malaria Control Programme reported incidence in Senegal Malaria J. (IF 3.0) Pub Date : 2024-03-05 Wesley Wong, Stephen F. Schaffner, Julie Thwing, Mame Cheikh Seck, Jules Gomis, Younouss Diedhiou, Ngayo Sy, Medoune Ndiop, Fatou Ba, Ibrahima Diallo, Doudou Sene, Mamadou Alpha Diallo, Yaye Die Ndiaye, Mouhamad Sy, Aita Sene, Djiby Sow, Baba Dieye, Abdoulaye Tine, Jessica Ribado, Joshua Suresh, Albert Lee, Katherine E. Battle, Joshua L. Proctor, Caitlin A. Bever, Bronwyn MacInnis, Daouda Ndiaye, Daniel
Genetic surveillance of the Plasmodium falciparum parasite shows great promise for helping National Malaria Control Programmes (NMCPs) assess parasite transmission. Genetic metrics such as the frequency of polygenomic (multiple strain) infections, genetic clones, and the complexity of infection (COI, number of strains per infection) are correlated with transmission intensity. However, despite these
-
Insecticide-treated bed nets and residual indoor spraying reduce malaria in areas with low transmission: a reanalysis of the Maltrials study Malaria J. (IF 3.0) Pub Date : 2024-03-04 Taye Gari, Bernt Lindtjørn
The malaria incidence data from a malaria prevention study from the Rift Valley, Central Ethiopia, were reanalysed. The objective was to investigate whether including an administrative structure within the society, which may have required consideration in the protocol or previous analysis, would provide divergent outcomes on the effect measures of the interventions. A cluster-randomized controlled
-
Early morning anopheline mosquito biting, a potential driver of malaria transmission in Busia County, western Kenya Malaria J. (IF 3.0) Pub Date : 2024-03-04 Julius I. Odero, Bernard Abong’o, Vincent Moshi, Sheila Ekodir, Steven A. Harvey, Eric Ochomo, John E. Gimnig, Nicole L. Achee, John P. Grieco, Prisca A. Oria, April Monroe
Insecticide-treated nets (ITNs) contributed significantly to the decline in malaria since 2000. Their protective efficacy depends not only on access, use, and net integrity, but also location of people within the home environment and mosquito biting profiles. Anopheline mosquito biting and human location data were integrated to identify potential gaps in protection and better understand malaria transmission
-
Adult mosquitoes of the sibling species Anopheles gambiae and Anopheles coluzzii exhibit contrasting patterns of susceptibility to four neonicotinoid insecticides along an urban-to-rural gradient in Yaoundé, Cameroon Malaria J. (IF 3.0) Pub Date : 2024-03-02 Fred A. Ashu, Caroline Fouet, Marilene M. Ambadiang, Véronique Penlap-Beng, Colince Kamdem
Neonicotinoids are potential alternatives for controlling pyrethroid-resistant mosquitoes, but their efficacy against malaria vector populations of sub-Saharan Africa has yet to be investigated. The aim of the present study was to test the efficacy of four neonicotinoids against adult populations of the sibling species Anopheles gambiae and Anopheles coluzzii sampled along an urban-to-rural gradient
-
Progress towards malaria elimination in the Greater Mekong Subregion: perspectives from the World Health Organization Malaria J. (IF 3.0) Pub Date : 2024-03-01 Giulia Manzoni, Rady Try, Jean Olivier Guintran, Céline Christiansen-Jucht, Elodie Jacoby, Siv Sovannaroth, Zaixing Zhang, Vilasack Banouvong, Matthew Scott Shortus, Rita Reyburn, Chitsavang Chanthavisouk, Nay Yi Yi Linn, Badri Thapa, San Kyawt Khine, Prayuth Sudathip, Deyer Gopinath, Nguyen Quang Thieu, Mya Sapal Ngon, Dai Tran Cong, Liu Hui, James Kelley, Neena Nee Kesar Valecha, Maria Dorina Bustos
Malaria remains a global health challenge, disproportionately affecting vulnerable communities. Despite substantial progress, the emergence of anti-malarial drug resistance poses a constant threat. The Greater Mekong Subregion (GMS), which includes Cambodia, China’s Yunnan province, Lao People's Democratic Republic, Myanmar, Thailand, and Viet Nam has been the epicentre for the emergence of resistance
-
Correction: Testing and treatment for malaria elimination: a systematic review Malaria J. (IF 3.0) Pub Date : 2024-03-01 Gretchen Newby, Chris Cotter, Michelle E. Roh, Kelly Harvard, Adam Bennett, Jimee Hwang, Nakul Chitnis, Sydney Fine, Gillian Stresman, Ingrid Chen, Roly Gosling, Michelle S. Hsiang
Correction: Malaria Journal (2023) 22:254 https://doi.org/10.1186/s12936-023-04670-8 Following publication of the original article [1], the authors flagged two sets of errors (one in the Results and the other in Table 2) and made one clarification (in the caption of Figure 4): The first error arose because they updated Fig. 3 prior to submission but neglected to update the accompanying text; the second
-
Malaria elimination and the need for intensive inter-country cooperation. a critical evaluation of regional technical co-operation in Southern Africa Malaria J. (IF 3.0) Pub Date : 2024-02-28 Chadwick H. Sikaala, Bongani Dlamini, Alphart Lungu, Phelele Fakudze, Mukosha Chisenga, Chishala Lukwesa Siame, Nyasha Mwendera, Dumisani Shaba, John M. Chimumbwa, Immo Kleinschmidt
Malaria elimination requires closely co-ordinated action between neighbouring countries. In Southern Africa several countries have reduced malaria to low levels, but the goal of elimination has eluded them thus far. The Southern Africa Development Community (SADC) Malaria Elimination Eight (E8) initiative was established in 2009 between Angola, Botswana, Eswatini, Mozambique, Namibia, South Africa
-
Safety and efficacy of pyronaridine–artesunate paediatric granules in the treatment of uncomplicated malaria in children: insights from randomized clinical trials and a real-world study Malaria J. (IF 3.0) Pub Date : 2024-02-28 Michael Ramharter, Abdoulaye A. Djimde, Isabelle Borghini-Fuhrer, Robert Miller, Jangsik Shin, Adam Aspinall, Naomi Richardson, Martina Wibberg, Lawrence Fleckenstein, Sarah Arbe-Barnes, Stephan Duparc
Children are particularly at risk of malaria. This analysis consolidates the clinical data for pyronaridine–artesunate (PA) paediatric granules in children from three randomized clinical trials and a real-world study (CANTAM). An integrated safety analysis of individual patient data from three randomized clinical trials included patients with microscopically-confirmed Plasmodium falciparum, body weight ≥ 5 kg
-
Sibling species of the major malaria vector Anopheles gambiae display divergent preferences for aquatic breeding sites in southern Nigeria Malaria J. (IF 3.0) Pub Date : 2024-02-27 Faith I. Ebhodaghe, Irma Sanchez-Vargas, Clement Isaac, Brian D. Foy, Elizabeth Hemming-Schroeder
When integrated with insecticide-treated bed nets, larval control of Anopheles mosquitoes could fast-track reductions in the incidence of human malaria. However, larval control interventions may deliver suboptimal outcomes where the preferred breeding places of mosquito vectors are not well known. This study investigated the breeding habitat choices of Anopheles mosquitoes in southern Nigeria. The objective
-
How using light touch immersion research revealed important insights into the lack of progress in malaria elimination in Eastern Indonesia Malaria J. (IF 3.0) Pub Date : 2024-02-27 Dee Jupp, Sherria Ayuandini, Frisca Tobing, Denny Halim, Enny Kenangalem, Maria E. Sumiwi, Hellen D. Prameswari, Minerva Theodora, Hermawan Susanto, Riskha T. P. Dewi, Dedy Supriyanto, Bayu Kurnia, Mrunal Shetye, Ermi Ndoen, Yuka Onishi
By 2022, the Government of Indonesia had successfully eliminated malaria in 389 out of 514 districts but continues to face a challenge in Eastern Indonesia where 95% of the total 2021 malaria cases were reported from Papua, West Papua and Nusa Tenggara Timur provinces. There is an increased recognition that malaria elimination will require a better understanding of the human behavioural factors hindering
-
Competency of malaria laboratory diagnosis at national and provincial levels at the beginning of malaria post-elimination phase, China Malaria J. (IF 3.0) Pub Date : 2024-02-26 He Yan, Mei Li, Zhi-gui Xia, Jian-hai Yin
Qualified malaria diagnosis competency has contributed to the great achievement of malaria elimination in China. After eliminating malaria, it is still critical to the prevention of re-establishment of malaria transmission in China. This study was aimed to assess the malaria detection competency at national and provincial levels in China at the beginning of malaria post-elimination phase. In the present
-
Bayesian spatio-temporal analysis of malaria prevalence in children between 2 and 10 years of age in Gabon Malaria J. (IF 3.0) Pub Date : 2024-02-23 Fabrice Mougeni, Bertrand Lell, Ngianga-Bakwin Kandala, Tobias Chirwa
Gabon still bears significant malaria burden despite numerous efforts. To reduce this burden, policy-makers need strategies to design effective interventions. Besides, malaria distribution is well known to be related to the meteorological conditions. In Gabon, there is limited knowledge of the spatio-temporal effect or the environmental factors on this distribution. This study aimed to investigate
-
Radical cure for Plasmodium vivax malaria after G6PD qualitative testing in four provinces in Cambodia, results from Phase I implementation Malaria J. (IF 3.0) Pub Date : 2024-02-23 Dysoley Lek, Yu-Cheng Tsai, Jillian Hirano, Siv Sovannaroth, Voeurng Bunreth, Prak Vonn, Or Vannthen, Tol Bunkea, Top Samphornarann, Nguon Sokomar, Mak Sarath, Soy Ty Kheang, Evelyn Wong, Michelle K. Burbach, Jayme Hughes, Huy Rekol
Cambodia aims to eliminate all forms of malaria by 2025. In 2020, 90% of all malaria cases were Plasmodium vivax. Thus, preventing P. vivax and relapse malaria is a top priority for elimination. 14-day primaquine, a World Health Organization-recommended radical cure treatment regimen, specifically targets dormant hypnozoites in the liver to prevent relapse. Cambodia introduced P. vivax radical cure
-
Genetic differentiation of Plasmodium vivax duffy binding protein in Ethiopia and comparison with other geographical isolates Malaria J. (IF 3.0) Pub Date : 2024-02-23 Abnet Abebe, Cheikh Cambel Dieng, Sisay Dugassa, Deriba Abera, Tassew T. Shenkutie, Ashenafi Assefa, Didier Menard, Eugenia Lo, Lemu Golassa
Plasmodium vivax Duffy binding protein (PvDBP) is a merozoite surface protein located in the micronemes of P. vivax. The invasion of human reticulocytes by P. vivax merozoites depends on the parasite DBP binding domain engaging Duffy Antigen Receptor for Chemokine (DARC) on these red blood cells (RBCs). PvDBPII shows high genetic diversity which is a major challenge to its use in the development of