样式: 排序: IF: - GO 导出 标记为已读
-
Global mapping of antibiotic resistance rates among clinical isolates of Stenotrophomonas maltophilia: a systematic review and meta-analysis Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-03-19 Narjess Bostanghadiri, Mohammad Sholeh, Tahereh Navidifar, Leila Dadgar-Zankbar, Zahra Elahi, Alex van Belkum, Davood Darban-Sarokhalil
Infections caused by Stenotrophomonas maltophilia are clinically important due to its intrinsic resistance to a broad range of antibiotics. Therefore, selecting the most appropriate antibiotic to treat S. maltophilia infection is a major challenge. The current meta-analysis aimed to investigate the global prevalence of antibiotic resistance among S. maltophilia isolates to the develop more effective
-
Bigger problems from smaller colonies: emergence of antibiotic-tolerant small colony variants of Mycobacterium avium complex in MAC-pulmonary disease patients Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-03-18 Hyun-Eui Park, Kyu-Min Kim, Minh Phuong Trinh, Jung-Wan Yoo, Sung Jae Shin, Min-Kyoung Shin
Mycobacterium avium complex (MAC) is a group of slow-growing mycobacteria that includes Mycobacterium avium and Mycobacterium intracellulare. MAC pulmonary disease (MAC-PD) poses a threat to immunocompromised individuals and those with structural pulmonary diseases worldwide. The standard treatment regimen for MAC-PD includes a macrolide in combination with rifampicin and ethambutol. However, the treatment
-
Coexistence of blaIMP−4 and blaSFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-03-06 Hui Chen, Hao Xu, Ruishan Liu, Jian Shen, Beiwen Zheng, Lanjuan Li
Klebsiella variicola is considered a newly emerging human pathogen. Clinical isolates of carbapenemase and broad-spectrum β-lactamase-producing K. variicola remain relatively uncommon. A strain of K. variicola 4253 was isolated from a clinical sample, and was identified to carry the blaIMP−4 and blaSFO−1 genes. This study aims to discern its antibiotic resistance phenotype and genomic characteristics
-
Clinical characteristics and drug resistance of Nocardia in Henan, China, 2017–2023 Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-03-06 Yungang Han, Meijin Cheng, Zheng Li, Huihui Chen, Shuang Xia, Yue Zhao, Yali Wang, Wenyi He, Wei Wang
The aim of this study was to investigate the clinical features of Nocardia infections, antibiotic resistance profile, choice of antibiotics and treatment outcome, among others. In addition, the study compared the clinical and microbiological characteristics of nocardiosis in bronchiectasis patients and non-bronchiectasis patients. Detailed clinical data were collected from the medical records of 71
-
Nanopore targeted sequencing-based diagnosis of central nervous system infections in HIV-infected patients Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-29 Xihong Yang, Shuilian Zhou, Ziwei Chang, Xiaotong Xi, Jiahui Li, Mengjiao Miao, Yaling Chen, Wei Chen, Hongying Zhang, Ran Ding, Zhiliang Hu
Early and accurate etiological diagnosis is very important for improving the prognosis of central nervous system (CNS) infections in human immunodeficiency virus (HIV)-infected patients. The goal is not easily achieved by conventional microbiological tests. We developed a nanopore targeted sequencing (NTS) platform and evaluated the diagnostic performance for CNS infections in HIV-infected patients
-
Aetiology and use of antibiotics in pregnancy-related infections: results of the WHO Global Maternal Sepsis Study (GLOSS), 1-week inception cohort Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-24 Carolina C. Ribeiro-do-Valle, Mercedes Bonet, Vanessa Brizuela, Edgardo Abalos, Adama Baguiya, Fernando Bellissimo-Rodrigues, Mihaela Budianu, Lucian Puscasiu, Marian Knight, David Lissauer, Catherine Dunlop, Shevin T. Jacob, Sadia Shakoor, Luis Gadama, Bouchra Assarag, João Paulo Souza, Jose G. Cecatti
Pregnancy-related infections are important contributors to maternal sepsis and mortality. We aimed to describe clinical, microbiological characteristics and use of antibiotics by source of infection and country income, among hospitalized women with suspected or confirmed pregnancy-related infections. We used data from WHO Global Maternal Sepsis Study (GLOSS) on maternal infections in hospitalized women
-
Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-24 Sara A. Alshaikh, Tarek El-banna, Fatma Sonbol, Mahmoud H. Farghali
Uropathogenic Escherichia coli (UPEC) is the main etiological agent behind community-acquired and hospital-acquired urinary tract infections (UTIs), which are among the most prevalent human infections. The management of UPEC infections is becoming increasingly difficult owing to multi-drug resistance, biofilm formation, and the possession of an extensive virulence arsenal. This study aims to characterize
-
Characterization of resistance genes and replicon typing in Carbapenem-resistant Klebsiella pneumoniae strains Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-24 Esin Karaman, Ayşegül Çopur Çiçek, Vicdan Şemen, Fatih Şaban Beriş
In our study, K. pneumoniae strains (non-susceptible to carbapenem) (n = 60) were obtained from various clinical samples from Rize State Hospital between 2015 and 2017 and it is aimed to identify antibiotic resistance genes and replicon typing. Antibiotic susceptibility tests of the strains were performed with Kirby-Bauer disk diffusion test and the Vitek-2 automated system (BioMerieux, France). Antibiotic
-
Molecular epidemiological characteristics of osteoarthritis-associated Brucella melitensis in China: evidence from whole-genome sequencing-based analysis Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-24 Lei Zhu, Chi Zhang, Chen Liang, Li Peng, Huanyu Yan, Xiuwen Liang, Youjia Xu
Brucellosis, developing complications including arthritis, spondylitis, sacroiliitis, and osteomyelitis, is one of the most common zoonotic diseases in the current world which causes economic losses to the livestock industry and is a great public health concern. Brucella melitensis are the main pathogen of brucellosis epidemics in China, most of which are located in northern China. However, there is
-
Genomic characterization, in vitro, and preclinical evaluation of two microencapsulated lytic phages VB_ST_E15 and VB_ST_SPNIS2 against clinical multidrug-resistant Salmonella serovars Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-15 Reem A. Youssef, Masarra M. Sakr, Rania I. Shebl, Bishoy T. Saad, Khaled M. Aboshanab
Salmonella infections continue to be one of the essential public health issues threatening millions of people. With the increasing occurrence of resistance against conventionally used antibiotics, the search for alternatives has become crucial. In this study, we aimed to isolate, characterize, and evaluate two lytic bacteriophages against clinically isolated multidrug-resistant (MDR) Salmonella serovars
-
Application of the Weighted-Incidence Syndromic Combination Antibiogram (WISCA) to guide the empiric antibiotic treatment of febrile neutropenia in oncological paediatric patients: experience from two paediatric hospitals in Northern Italy Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-15 Cecilia Liberati, Daniele Donà, Linda Maestri, Maria Grazia Petris, Elisa Barbieri, Elisa Gallo, Jacopo Gallocchio, Marta Pierobon, Elisabetta Calore, Annachiara Zin, Giulia Brigadoi, Marcello Mariani, Alessio Mesini, Carolina Saffioti, Elisabetta Ugolotti, Dario Gregori, Carlo Giaquinto, Elio Castagnola, Alessandra Biffi
Guidelines about febrile neutropenia in paediatric patients are not homogeneous; the best empiric treatment of this condition should be driven by local epidemiology. The Weighted-Incidence Syndromic Combination Antibiogram (WISCA) addresses the need for disease-specific local susceptibility evidence that could guide empiric antibiotic prescriptions based on outcome estimates of treatment regimens obtained
-
Epidemiology and outcomes of multidrug-resistant bacterial infection in non-cystic fibrosis bronchiectasis Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-13 Chih-Hao Chang, Chiung-Hsin Chang, Shih-Hao Huang, Chung-Shu Lee, Po-Chuan Ko, Chun-Yu Lin, Meng-Heng Hsieh, Yu-Tung Huang, Horng-Chyuan Lin, Li-Fu Li, Fu-Tsai Chung, Chun-Hua Wang, Hung-Yu Huang
Multidrug-resistant (MDR) bacteria impose a considerable health-care burden and are associated with bronchiectasis exacerbation. This study investigated the clinical outcomes of adult patients with bronchiectasis following MDR bacterial infection. From the Chang Gung Research Database, we identified patients with bronchiectasis and MDR bacterial infection from 2008 to 2017. The control group comprised
-
Co-transfer of IncFII/IncFIB and IncFII plasmids mediated by IS26 facilitates the transmission of mcr-8.1 and tmexCD1-toprJ1 Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-13 Qian Wang, Meng Zhang, Yue Liu, Jinmei Li, Ran Chen, Yueling Wang, Yan Jin, Yuanyuan Bai, Zhen Song, Xinglun Lu, Changyin Wang, Yingying Hao
This study aimed to characterise the whole-genome structure of two clinical Klebsiella pneumoniae strains co-harbouring mcr-8.1 and tmexCD1-toprJ1, both resistant to colistin and tigecycline. K. pneumoniae strains TGC-02 (ST656) and TGC-05 (ST273) were isolated from urine samples of different patients hospitalised at separate times in 2021. Characterisation involved antimicrobial susceptibility testing
-
Molecular characterization of extensively drug-resistant hypervirulent Pseudomonas aeruginosa isolates in China Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-12 Jun Li, Mengli Tang, Zhaojun Liu, Yuhan Wei, Fengjun Xia, Yubing Xia, Yongmei Hu, Haichen Wang, Mingxiang Zou
Recently, extensively drug-resistant Pseudomonas aeruginosa (XDR-PA) isolates have been increasingly detected and posed great challenges to clinical anti-infection treatments. However, little is known about extensively resistant hypervirulent P. aeruginosa (XDR-hvPA). In this study, we investigate its epidemiological characteristics and provide important basis for preventing its dissemination. Clinical
-
Antimicrobial resistance survey and whole-genome analysis of nosocomial P. Aeruginosa isolated from eastern Province of China in 2016–2021 Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-09 Zimeng Hu, Lu Zhou, Xingyu Tao, Pei Li, Xiangkuan Zheng, Wei Zhang, Zhongming Tan
Pseudomonas aeruginosa is a major Gram-negative pathogen that can exacerbate lung infections in the patients with cystic fibrosis, which can ultimately lead to death. From 2016 to 2021, 103 strains of P. aeruginosa were isolated from hospitals and 20 antibiotics were used for antimicrobial susceptibility determination. Using next-generation genome sequencing technology, these strains were sequenced
-
Integrated approach for detection of SARS-CoV-2 and its variant by utilizing LAMP and ARMS-PCR Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-01 Maryam Nawab, Syeda Kiran Riaz, Eiman Ismail, Alfar Ahamed, Aaysha Tariq, Muhammad Faraz Arshad Malik, Naeem F. Qusty, Farkad Bantun, Petr Slama, Massab Umair, Shafiul Haque, D. Katterine Bonilla-Aldana, Alfonso J. Rodriguez-Morales
Global impact of COVID-19 pandemic has heightened the urgency for efficient virus detection and identification of variants such as the Q57H mutation. Early and efficient detection of SARS-CoV-2 among densely populated developing countries is paramount objective. Although RT-PCR assays offer accuracy, however, dependence on expansive kits and availability of allied health resources pose an immense challenge
-
Diagnostic strategy of metagenomic next-generation sequencing for gram negative bacteria in respiratory infections Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-02-01 Wenyan Liang, Qun Zhang, Qian Qian, Mingyue Wang, Yuchen Ding, Ji Zhou, Yi Zhu, Yu Jin, Xuesong Chen, Hui Kong, Wei Song, Xin Lu, Xiaodong Wu, Xiaoyong Xu, Shanling Dai, Wenkui Sun
This study aims to identify the most effective diagnostic method for distinguishing pathogenic and non-pathogenic Gram-negative bacteria (GNB) in suspected pneumonia cases using metagenomic next-generation sequencing (mNGS) on bronchoalveolar lavage fluid (BALF) samples. The effectiveness of mNGS was assessed on BALF samples collected from 583 patients, and the results were compared with those from
-
Dissemination of mcr-1 and β-lactamase genes among Pseudomonas aeruginosa: molecular characterization of MDR strains in broiler chicks and dead-in-shell chicks infections Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-01-28 Mona Salem, Gamal Younis, Asmaa Sadat, Nehal Ahmed Talaat Nouh, Dalal Nasser Binjawhar, Mohamed M. Abdel-Daim, Mohamed Elbadawy, Amal Awad
Pseudomonas aeruginosa (P. aeruginosa) is one of the most serious pathogens implicated in antimicrobial resistance, and it has been identified as an ESKAPE along with other extremely significant multidrug resistance pathogens. The present study was carried out to explore prevalence, antibiotic susceptibility phenotypes, virulence-associated genes, integron (int1), colistin (mcr-1), and β-lactamase
-
Pulmonary co-infections by Pneumocystis jirovecii and Herpesviridae: a seven-year retrospective study Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-01-20 Alan Rucar, Anne Totet, Yohann Le Govic, Baptiste Demey, Céline Damiani
Pneumocystis jirovecii (P. jirovecii) is an opportunistic fungus responsible for Pneumocystis pneumonia (PCP) in deeply immunocompromised patients and for pulmonary colonization in individuals with mild immunosuppression or impaired respiratory function. PCP and Cytomegalovirus (CMV) co-infections have been widely described whereas those involving other Herpesviruses (HVs) such as Epstein-Barr virus
-
Combination antimicrobial therapy: in vitro synergistic effect of anti-staphylococcal drug oxacillin with antimicrobial peptide nisin against Staphylococcus epidermidis clinical isolates and Staphylococcus aureus biofilms Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-01-20 Toktam Sharafi, Ezzat Allah Ghaemi, Maryam Rafiee, Abdollah Ardebili
The ability of Staphylococcus epidermidis and S. aureus to form strong biofilm on plastic devices makes them the major pathogens associated with device-related infections (DRIs). Biofilm-embedded bacteria are more resistant to antibiotics, making biofilm infections very difficult to effectively treat. Here, we evaluate the in vitro activities of anti-staphylococcal drug oxacillin and antimicrobial
-
Molecular characterization of extended-spectrum β-lactamase-producing Escherichia coli isolated from lower respiratory tract samples between 2002 and 2019 in the Central Slovenia region Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-01-13 Katja Hrovat, Katja Molan, Katja Seme, Jerneja Ambrožič Avguštin
Antibiotic resistance is one of the most serious global health problems and threatens the effective treatment of bacterial infections. Of greatest concern are infections caused by extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC). The aim of our study was to evaluate the prevalence and molecular characteristics of ESBL-EC isolated over an 18-year pre-COVID period from lower respiratory
-
Genomic diversity of β-lactamase producing Pseudomonas aeruginosa in Iran; the impact of global high-risk clones Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-01-13 Nazila Ahmadi, Himen Salimizand, Abolfazl Rafati Zomorodi, Jalileh Ebn Abbas, Rashid Ramazanzadeh, Fakhri Haghi, Sepideh Hassanzadeh, Mojdeh Jahantigh, Mojtaba Shahin
Hospital-acquired infections caused by multidrug-resistant Pseudomonas aeruginosa incline hospital stay and costs of treatment that resulted in an increased mortality rate. The frequency of P. aeruginosa high-risk clones producing carbapenemases was investigated in our clinical samples. In this cross-sectional study, 155 non-repetitive P. aeruginosa isolates were included from different medical centers
-
Impact of Helicobacter pylori colonization density and depth on gastritis severity Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-01-12 Jianxiang Peng, Jinliang Xie, Dingwei Liu, Kaijie Yang, Shuang Wu, Dongsheng Liu, Deqiang Huang, Yong Xie
Helicobacter pylori (H. pylori) infection is the most common etiology of chronic gastric. H. pylori gastritis would gradually evolve into gastric atrophy, intestinal metaplasia, dysplasia and malignant lesions. Herein, this study aimed to investigate the potential impact of H. pylori colonization density and depth on the severity of histological parameters of gastritis. A prospective monocentric study
-
The role of rapid syndromic diagnostic testing of gastrointestinal pathogens as a clinical decision support tool in a pediatric emergency department Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-01-05 Hyun Mi Kang, In Hyuk Yoo, Dae Chul Jeong
This study aimed to investigate the role of rapid syndromic diagnostic testing of gastrointestinal pathogens as a clinical decision support tool in a pediatric emergency department (ED) by comparing clinical decision and patient outcome parameters pre- and post-implementation. This was a big data analytical study of children < 18 years old without any underlying diseases, that visited the ED with acute
-
Prevalence estimation of Tropheryma whipplei in duodenal biopsy tissues of Koreans Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-01-03 Sumi Yoon, Min Eui Hong, Soon Auck Hong, Tae-Hyoung Kim, Mi-Kyung Lee
Whipple’s disease caused by Tropheryma whipplei is difficult to diagnose because of a broad spectrum of manifestations and non-specific clinical signs. In the current global era, the incidence of duodenal infection/inflammation caused by T. whipplei in Korea may has been underestimated. Here we estimated the prevalence of T. whipplei in duodenal biopsy tissues of Koreans using real-time PCRs (RT-PCRs)
-
Changes in tuberculosis risk after transplantation in the setting of decreased community tuberculosis incidence: a national population-based study, 2008–2020 Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2024-01-03 JongHoon Hyun, Myeongjee Lee, Inkyung Jung, Eunhwa Kim, Seung Min Hahn, Yu Ri Kim, Sungmin Lim, Kyong Ihn, Min Young Kim, Jong Gyun Ahn, Joon-Sup Yeom, Su Jin Jeong, Ji-Man Kang
Transplant recipients are immunocompromised and vulnerable to developing tuberculosis. However, active tuberculosis incidence is rapidly declining in South Korea, but the trend of tuberculosis infection among transplant recipients has not been elucidated. This study aimed to evaluate the risk of active tuberculosis after transplantation, including risk factors for tuberculosis and standardized incidence
-
Recombination-mediated dissemination of Methicillin-resistant S. aureus clonal complex 1 in the Egyptian health care settings Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-12-14 Salma W. Elsayed, Reem A. Elghaish, Eman Badr, Shaimaa F. Mouftah, Nehal A. Saif, Iman S. Naga, Ahmed H. Shata, Ben Pascoe, Samuel K. Sheppard, Mohamed Elhadidy
Methicillin-resistant Staphylococcus aureus (MRSA) is a rapidly evolving pathogen that is frequently associated with outbreaks and sustained epidemics. This study investigated the population structure, resistome, virulome, and the correlation between antimicrobial resistance determinants with phenotypic resistance profiles of 36 representative hospital-acquired MRSA isolates recovered from hospital
-
Implementation of a PCR-based strategy to control an outbreak by Serratia marcescens in a Neonatal Intensive Care Unit Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-12-11 Ángel Rodríguez-Villodres, José Manuel Ortiz de la Rosa, Raquel Valencia-Martin, Francisco Jiménez Parrilla, Guillermo Martín-Gutiérrez, Natividad Márquez Patiño, Estela Perea Cruz, María Teresa Sánchez Jiménez, Antonio Pavón Delgado, José Miguel Cisneros, José Antonio Lepe
To evaluate the clinical and epidemiological impact of a new molecular surveillance strategy based on qPCR to control an outbreak by Serratia marcescens in a Neonatal Intensive Care Unit (NICU). We design a specific qPCR for the detection of S. marcescens in rectal swabs of patients admitted to a NICU. We divided the surveillance study into two periods: (a) the pre-PCR, from the outbreak declaration
-
Comparison of the inoculum effect of in vitro antibacterial activity of Imipenem/relebactam and Ceftazidime/avibactam against ESBL-, KPC- and AmpC-producing Escherichia coli and Klebsiella pneumoniae Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-12-10 Xueting Wang, Luying Xiong, Yuan Wang, Kai Yang, Tingting Xiao, Xiaohui Chi, Tao Chen, Yanzi Zhou, Ping Lu, Dilimulati Dilinuer, Pin Shen, Yunbo Chen, Yonghong Xiao
To evaluate effect of inoculum size of extended-spectrum β-Lactamase (ESBL)-producing-, AmpC-producing-, and KPC-producing Escherichia coli and Klebsiella pneumoniae on the in vitro antibacterial effects of imipenem/relebactam (IMR) and ceftazidime/avibactam (CZA). We compared the impact of inoculum size on IMR and CZA of sixteen clinical isolates and three standard isolates through antimicrobial susceptibility
-
Effectiveness and safety of regimens containing linezolid for treatment of Mycobacterium abscessus pulmonary Disease Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-12-06 Li-ping Cheng, Qing Zhang, Hai Lou, Xiao-na Shen, Qing-rong Qu, Jie Cao, Wei Wei, Wei Sha, Qin Sun
To evaluate the effectiveness and safety of linezolid-containing regimens for treatment of M. abscessus pulmonary disease. The records of 336 patients with M. abscessus pulmonary disease who were admitted to Shanghai Pulmonary Hospital from January 2018 to December 2020 were retrospectively analyzed. A total of 164 patients received a linezolid-containing regimen and 172 controls did not. The effectiveness
-
Therapeutical strategies in cavitary legionnaires’ disease, two cases from the field and a systematic review Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-29 Marco Moretti, Lisanne De Boek, Bart Ilsen, Thomas Demuyser, Eef Vanderhelst
Legionnaires’ Disease (LD) rarely evolves into pulmonary abscesses. The current systematic review has been designed to explore therapeutical strategies in pulmonary cavitary LD. A research strategy was developed and applied to the databases Embase, Pubmed, and Web of Science from the 1st of January 2000 to the 1st of November 2022. Original articles, case series, case reports, and guidelines written
-
Clinical and molecular characteristics of methicillin-resistant Staphylococcus aureus in bone and joint infection among children Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-22 Pan Fu, Yaxier Nijiati, Tingting Li, Xia Wu, Zixuan Wang, Jinlan Zhou, Chuanqing Wang, Bo Ning
To investigate the characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) in bone and joint infection (BJI) among children. A total of 338 patients diagnosed with BJI from 2013 to 2022 in Children’s Hospital of Fudan University were enrolled. Demographic information, microbiology culture results and laboratory findings, including white blood counts (WBC), C-reactive protein (CRP), procalcitonin
-
PCT, IL-6, and IL-10 facilitate early diagnosis and pathogen classifications in bloodstream infection Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-20 Xianggui Yang, Jun Zeng, Xuejing Yu, Zhenguo Wang, Dan Wang, Qin Zhou, Tingting Bai, Ying Xu
In the diagnosis of bloodstream infection (BSI), various inflammatory markers such as C-reactive protein (CRP), procalcitonin (PCT), interleukins (IL), white blood cell count (WBC), neutrophil percentage (NE%), platelet count (PLT), and erythrocyte sedimentation rate (ESR) have been extensively utilized. However, their specific roles in distinguishing BSI from local bacterial infection (LBI) and in
-
Negative serum (1,3) -β-D-glucan has a low power to exclude Pneumocystis jirovecii pneumonia (PJP) in HIV-uninfected patients with positive qPCR Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-20 Yuan Huang, Jie Yi, Jing-jing Song, Li-jun Du, Xiao-meng Li, Lin-lin Cheng, Song-xin Yan, Hao-long Li, Yong-mei Liu, Hao-ting Zhan, Ya-ling Dou, Yong-zhe Li
The current study evaluated the diagnostic performance of serum (1,3)-beta-D Glucan (BDG) in differentiating PJP from P. jirovecii-colonization in HIV-uninfected patients with P. jirovecii PCR-positive results. This was a single-center retrospective study between 2019 and 2021. The diagnosis of PJP was based on the following criteria: detection of P. jirovecii in sputum or BAL specimen by qPCR or microscopy;
-
Efficacy and nephrotoxicity of polymyxin B in elderly patients with carbapenem resistant bacterial infection Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-15 G. L. Xia, X. Xu, X. B. You, X. Wang, D. D. Feng, S. Lei, R. L. Jiang
To study the efficacy and nephrotoxicity of polymyxin B in the treatment of elderly patients with carbapenem-resistant organism (CRO) infection. The clinical and microbiological data of patients with CRO-infected sepsis treated with polymyxin B were retrospectively analyzed. The effective rate, bacterial clearance, incidence and recovery rate of acute renal injury (AKI) and prognosis-related indicators
-
Correction: Microbiological diagnosis of pulmonary invasive aspergillosis in critically ill patients with severe SARS-CoV-2 pneumonia: a bronchoalveolar study Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-09 Ángel Estella, Ignacio Martín-Loeches, María Recuerda Núñez, Clara González García, Liliana Marcela Pesaresi, Alvaro Antón Escors, Maria Dolores López Prieto, Juan Manuel Sánchez Calvo
Correction to: Annals of Clinical Microbiology and Antimicrobials (2023) 22:90 https://doi.org/10.1186/s12941-023-00626-7 Following publication of the original article [1], the author name “Ignacio Martín-Loeches” was incorrectly written as “Ignacio Martin Loeches”. This has now been corrected with this erratum. The original article has been corrected. Authors and Affiliations Intensive Care Unit University
-
The oral bacterial microbiota facilitates the stratification for ulcerative colitis patients with oral ulcers Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-09 Jun Xu, Yu Zhang, Xiao-Hui Fang, Yun Liu, Yi-Bo Huang, Zi-Liang Ke, Yang Wang, Yi-Fan Zhang, Yang Zhang, Jian-Hua Zhou, Hui-Ting Su, Ning Chen, Yu-Lan Liu
Clinically, a large part of inflammatory bowel disease (IBD) patients is complicated by oral lesions. Although previous studies proved oral microbial dysbiosis in IBD patients, the bacterial community in the gastrointestinal (GI) tract of those IBD patients combined with oral ulcers has not been profiled yet. In this study, we enrolled four groups of subjects, including healthy controls (CON), oral
-
Unveiling the etiology of peritonsillar abscess using next generation sequencing Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-08 Merili Saar, Risto Vaikjärv, Ülle Parm, Priit Kasenõmm, Siiri Kõljalg, Epp Sepp, Madis Jaagura, Andres Salumets, Jelena Štšepetova, Reet Mändar
Peritonsillar abscess (PTA) is a severe deep neck space infection with an insufficiently characterized bacterial etiology. We aimed to reveal the bacteria associated with PTA applying next generation sequencing (NGS). Tonsil biopsies and pus samples of 91 PTA patients were analysed applying NGS method. Over 400 genera and 800 species belonging to 34 phyla were revealed. The most abundant species in
-
Catheter-related bloodstream infection caused by Tsukamurella tyrosinosolvens identified by secA1sequencing in an immunocompromised child: a case report Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-08 Shinsuke Mizuno, Yoshiyuki Tsukamura, Shuro Nishio, Toshiaki Ishida, Daiichiro Hasegawa, Yoshiyuki Kosaka, Tadasuke Ooka, Junichiro Nishi, Masashi Kasai
Tsukamurella spp. are obligate aerobic, gram-positive, non-motile, and slightly acid-fast bacilli belonging to the Actinomycetes family. They share many characteristics with Nocardia, Rhodococcus, Gordonia, and the rapidly growing Mycobacterium species. Therefore, standard testing may misidentify Tsukamurella spp. as another species. Accurate and rapid diagnosis is critical for proper infection management
-
Bacterial profile of surgical site infection and antimicrobial resistance patterns in Ethiopia: a multicentre prospective cross-sectional study Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-07 Seble Worku, Tamrat Abebe, Ashenafi Alemu, Berhanu Seyoum, Göte Swedberg, Alemseged Abdissa, Adane Mihret, Getachew Tesfaye Beyene
Globally, surgical site infections (SSI) are the most commonly reported healthcare-associated infections. A multicentre study was conducted among patients who underwent surgical procedures at four hospitals located in Northern (Debre Tabor), Southern (Hawassa), Southwest (Jimma), and Central (Tikur Anbessa) parts of Ethiopia. A total of 752 patients clinically studied for surgical site infection were
-
A critical role of outer membrane vesicles in antibiotic resistance in carbapenem-resistant Klebsiella pneumoniae Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-11-02 Lifeng Yao, Beiwen Wei, Yuanxia Wang, Beihui Xu, Meng Yang, Xu Chen, Fuxiang Chen
This study aimed to illustrate the status of carbapenem-resistant Enterobacterales (CRE) infections in a Chinese tertiary hospital and to investigate the role of outer membrane vesicles (OMVs) in antibiotic resistance in carbapenem-resistant Klebsiella pneumoniae (CRKP). The data of CRE infections was collected from laboratory records, and the CRE isolates from two distinct periods (2015/07 to 2017/07
-
Rapid Mycobacterium abscessus antimicrobial susceptibility testing based on antibiotic treatment response mapping via Raman Microspectroscopy Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-10-30 Weicong Ren, Yuli Mao, Shanshan Li, Bo Gao, Xiaoting Fu, Xiaolu Liu, Pengfei Zhu, Yuanyuan Shang, Yuandong Li, Bo Ma, Luyang Sun, Jian Xu, Yu Pang
Antimicrobial susceptibility tests (ASTs) are pivotal tools for detecting and combating infections caused by multidrug-resistant rapidly growing mycobacteria (RGM) but are time-consuming and labor-intensive. We used a Mycobacterium abscessus-based RGM model to develop a rapid (24-h) AST from the beginning of the strain culture, the Clinical Antimicrobials Susceptibility Test Ramanometry for RGM (CAST-R-RGM)
-
Evaluating molecular epidemiology of carbapenem non-susceptible Klebsiella pneumoniae isolates with MLST, MALDI-TOF MS, PFGE Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-10-27 Yunus Emre Ibik, Nebahat Ejder, Elif Sevim, Erva Rakici, Elif Seren Tanriverdi, Aysegül Copur Cicek
This study aimed to evaluate antibiotic resistance genes and virulence genes and the clonal relationship of the carbapenem-nonsusceptible Klebsiella pneumoniae strains by molecular methods which are isolated from various clinical specimens from patients treated in tertiary care hospital in Turkey. Identification of 32 carbapenem non-susceptible K. pneumoniae were determined by VITEK-2 (BioMérieux,
-
Positive Francisella tularensis meningitis outcome despite delayed identification: a case report Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-10-24 Vesa Mäki-Koivisto, Marianne Korkala, Lotta Simola, Sonja Suutari-Kontio, Sini Koivunen, Teija Puhto, Ilkka S. Junttila
Francisella tularensis is a Gram-negative bacteria, that may cause a zoonotic disease, tularemia. Here, we describe a patient case, where a previously healthy young woman in Northern Finland contacted health care because of fever and headache. Due to the symptoms and lack of further diagnostic tools in primary health care, she was transferred to University Hospital (UH) where ampicillin and ceftriaxone
-
Extended-spectrum β-lactamase- producing gram-negative bacterial infections in severely ill COVID-19 patients admitted in a national referral hospital, Kenya Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-10-14 Jeniffer Munyiva Mutua, John Mwaniki Njeru, Abednego Moki Musyoki
Bacterial infections in COVID-19 patients, especially those caused by multidrug-resistant gram-negative strains, are associated with increased morbidity, hospital stay and mortality. However, there is limited data on the epidemiology of extended-spectrum β-lactamase (ESBL)-producing bacteria in COVID-19 patients. Here, we assessed the prevalence and the factors associated with ESBL-producing gram-negative
-
Microbiological diagnosis of pulmonary invasive aspergillosis in critically ill patients with severe SARS-CoV-2 pneumonia: a bronchoalveolar study Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-10-10 Ángel Estella, Ignacio Martín Loeches, María Recuerda Núñez, Clara González García, Liliana Marcela Pesaresi, Alvaro Antón Escors, Maria Dolores López Prieto, Juan Manuel Sánchez Calvo
Diagnosing COVID-19-associated pulmonary aspergillosis (CAPA) can be challenging since radiological and clinical criteria in the critically ill patient are nonspecific. Microbiological diagnostic support is therefore crucial. The aim of this study was to document the incidence of aspergillosis using bronchoalveolar lavage (BAL) as the diagnostic method and to determine the performance of the current
-
Effect of ZnO nanoparticles on biofilm formation and gene expression of the toxin-antitoxin system in clinical isolates of Pseudomonas aeruginosa Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-10-05 Hassan Valadbeigi, Nourkhoda Sadeghifard, Vahab Hassan Kaviar, Mohammad Hossein Haddadi, Sobhan Ghafourian, Abbas Maleki
Biofilm formation by Pseudomonas aeruginosa (P. aeruginosa) is known to be characteristic of this organism. This bacterium is considered one of the most life-threatening bacteria and has been identified as a priority pathogen for research by WHO. Biofilm-producing P. aeruginosa is a concern in many parts of the world due to antibiotic resistance. Alginate also plays an important role in the biofilm
-
“One Health” perspective on prevalence of co-existing extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae: a comprehensive systematic review and meta-analysis Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-22 Tsepo Ramatla, Tshepo Mafokwane, Kgaugelo Lekota, Maropeng Monyama, George Khasapane, Naledi Serage, Jane Nkhebenyane, Carlos Bezuidenhout, Oriel Thekisoe
The Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) bacterial isolates that produce extended-spectrum β-lactamases (ESBLs) contribute to global life-threatening infections. This study conducted a systematic review and meta-analysis on the global prevalence of ESBLs in co-existing E. coli and K. pneumoniae isolated from humans, animals and the environment. The systematic review
-
Clinical characteristics and drug susceptibility profiles of Mycobacterium abscessus complex infection at a medical school in Thailand Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-21 Songkiat Sukmongkolchai, Suthidee Petsong, Nont Oudomying, Ajala Prommi, Sunchai Payungporn, Warat Usawakidwiree, Kanphai Wongjarit, Gompol Suwanpimolkul, Kiatichai Faksri, Chusana Suankratay, Suwatchareeporn Rotcheewaphan
This study investigated the differences in epidemiological and clinical data, and antimicrobial susceptibilities among different subspecies of Mycobacterium abscessus complex (MABSC) clinical isolates at a medical school in Thailand. A total of 143 MABSC clinical isolates recovered from 74 patients were genotypically analyzed for erm(41), rrl, and rrs mutations, and antimicrobial susceptibilities were
-
High prevalence of Mucosa-Associated extended-spectrum β-Lactamase-producing Escherichia coli and Klebsiella pneumoniae among Iranain patients with inflammatory bowel disease (IBD) Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-14 Ayda Afshari Kharaghani, Naser Harzandi, Babak Khorsand, Mohsen Rajabnia, Azin Afshari Kharaghani, Hamidreza Houri
Several pieces of evidence suggest that certain pathobionts belonging to Enterobacterales are associated with the development and progression of inflammatory bowel diseases (IBD). Extended-spectrum β-lactamases (ESBLs) ESBLs are frequently found in the Enterobacterales members, particularly in Escherichia coli and Klebsiella spp., and might trigger antibiotic-induced perturbations of the intestinal
-
Multidrug-resistant Gram-negative bacterial infections and associated factors in a Kenyan intensive care unit: a cross-sectional study Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-14 Jane Wairimu Maina, Frank Gekara Onyambu, Peter Shikuku Kibet, Abednego Moki Musyoki
Patients admitted to intensive care units (ICU) are at risk of Gram-negative bacteria (GNB) infections, especially those caused by multidrug-resistant (MDR) isolates, increasing morbidity, mortality, and healthcare costs. However, epidemiological surveillance data on MDR bacteria to inform infection prevention and control (IPCs) interventions is limited in our study setting. Here we assessed the prevalence
-
Modulating the transcriptomic profile of multidrug-resistant Klebsiella pneumoniae biofilm formation by antibiotics in combination with zinc sulfate Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-12 Rania I. Shebl, Walid F. Elkhatib, Mona Shaban E. M. Badawy
Klebsiella pneumoniae is a significant healthcare-associated pathogen. We investigated the antimicrobial interaction pattern between zinc sulfate and antibiotics against K. pneumoniae biofilm on the phenotypic and genotypic levels. Determining the minimum biofilm inhibitory concentrations and the transcriptomic profile of K. pneumoniae biofilm formation genes post-treatment were carried out to evaluate
-
Tetracyclines resistance in Mycoplasma and Ureaplasma urogenital isolates derived from human: a systematic review and meta-analysis Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-11 Xiaoyan Wen, Mojgan Sarabi Nobakht, Yue Yang, Ebrahim Kouhsari, Sara Hajilari, Matin Zafar Shakourzadeh, Khalil Azizian
Urogenital Mycoplasma infections are considered an important public health problem, owing to the presence of antibiotic resistance or decreased susceptibility, the treatment options are limited. Therefore, this meta-analysis aimed to estimate resistance rates of genital Mycoplasmas to tetracyclines (tetracycline, doxycycline, and minocycline). We searched the relevant published studies in PubMed, Scopus
-
Genomic characterization of colistin-resistant Klebsiella pneumoniae isolated from intensive care unit patients in Egypt Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-09 Eriny T. Attalla, Amal M. Khalil, Azza S. Zakaria, Dave J. Baker, Nelly M. Mohamed
Egypt has witnessed elevated incidence rates of multidrug-resistant Klebsiella pneumoniae infections in intensive care units (ICUs). The treatment of these infections is becoming more challenging whilst colistin-carbapenem-resistant K. pneumoniae is upsurging. Due to the insufficiently available data on the genomic features of colistin-resistant K. pneumoniae in Egypt, it was important to fill in the
-
Face mask sampling (FMS) for tuberculosis shows lower diagnostic sensitivity than sputum sampling in Guinea Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-07 Souleymane Hassane-Harouna, Sofie Marijke Braet, Tom Decroo, Lansana Mady Camara, Alexandre Delamou, Sven de Bock, Nimer Ortuño-Gutiérrez, Gba-Foromo Cherif, Caroline M. Williams, Anika Wisniewska, Michael R. Barer, Leen Rigouts, Bouke Catherine de Jong
Pulmonary tuberculosis (PTB) diagnosis relies on sputum examination, a challenge in sputum-scarce patients. Alternative non-invasive sampling methods such as face mask sampling (FMS) have been proposed. To evaluate the value of FMS for PTB diagnosis by assessing its agreement with sputum samples processed by GeneXpert MTB/RIF (Ultra)(Xpert) testing, and describe FMS sensitivity and specificity. This
-
Epidemiological and clinical characteristics of psittacosis among cases with complicated or atypical pulmonary infection using metagenomic next-generation sequencing: a multi-center observational study in China Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-07 Weifeng Huang, Fengge Wang, Qingqing Cai, Huiliang Xu, Dengwei Hong, Han Wu, Lu Zhou, Linjie Hu, Yihan Lu
Chlamydia psittaci (C. psittaci) causes parrot fever in humans. Development of metagenomic next-generation sequencing (mNGS) enables the identification of C. psittaci. This study aimed to determine the epidemiological and clinical characteristics of parrot fever cases in China. A multi-center observational study was conducted in 44 tertiary and secondary hospitals across 14 provinces and municipalities
-
Emergence of carbapenem-resistant enterobacterales co-harboring blaOXA−78 and blaOXA−58 from India Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-07 Bhaskar Jyoti Das, K. Melson Singha, Jayalaxmi Wangkheimayum, Debadatta Dhar Chanda, Amitabha Bhattacharjee
Carbapenem-Resistant Enterobacterales (CRE) has been categorized as pathogens of critical priority by World Health organization (WHO) as they pose significant threat to global public health. Carbapenemase production considered as the principal resistance mechanism against carbapenems and with the recent surge and expansion of carbapenemases and its variants among clinically significant bacteria in
-
Correction: Evaluation of the liquid colony for identification and antimicrobial susceptibility testing directly from positive blood cultures Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-09-02 Calvo Maddalena, Migliorisi Giuseppe, Marianna Perez, Scalia Guido, Stefani Stefania
Correction to: Annals of Clinical Microbiology and Antimicrobials (2023) 22:72 https://doi.org/10.1186/s12941-023-00617-8 Following publication of the original article [1], the authors noticed some errors in the textual part. These has now been corrected with this erratum. 1. Among the results section of the abstract, the sentence should read “100% ID identification concordance was observed across
-
Secondary bacterial infections & extensively drug-resistant bacteria among COVID-19 hospitalized patients at the University Hospital in Kraków Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-08-24 A Pałka, A Kujawska, DA Hareza, M Gajda, Jerzy Wordliczek, E Jachowicz-Matczak, I Owsianka, B Żółtowska, A Chmielarczyk, D Romaniszyn, Gregorczyk-Maga I, J Wójkowska-Mach
Healthcare-associated infections (HAI) and bacterial antimicrobial resistance posed a therapeutic risk during the coronavirus disease 2019 (COVID-19) pandemic. The aim of this study was to analyze the HAIs in COVID-19 patients in the Intensive Care Unit (ICU) and non-ICU at the University Hospital in Krakow (UHK) with an emphasis on the susceptibility of the most frequently isolated pathogens and the
-
Dissemination of the high-risk cloneST147 carbapenem-resistant klebsiella pneumoniae from a local tertiary care hospital in the Republic of Korea Ann. Clin. Microbiol. Antimicrob. (IF 5.7) Pub Date : 2023-08-24 Jungsun Park, Eunkyung Shin, Gwang Rip Hwang, Min-Kyeong Kim, Seongjae Joo, Hyun Ju Jeong, Jin Seok Kim, Jaeil Yoo, Junyoung Kim
The emergence of carbapenem-resistant Enterobacterales (CRE) infections is rapidly increasing and represents a serious public threat. In 2020, a total of 16,883 carbapenemase-producing Enterobacterales strains were collected; among these isolates, 21 strains were repeatedly isolated in a local tertiary care hospital. Antimicrobial susceptibility testing was performed using the broth microdilution method