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Efficient solvent suppression with adiabatic inversion for 1H-detected solid-state NMR

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Abstract

This study introduces a conceptually new solvent suppression scheme with adiabatic inversion pulses for 1H-detected multidimensional solid-state NMR (SSNMR) of biomolecules and other systems, which is termed “Solvent suppression of Liquid signal with Adiabatic Pulse” (SLAP). 1H-detected 2D 13C/1H SSNMR data of uniformly 13C- and 15N-labeled GB1 sample using ultra-fast magic angle spinning at a spinning rate of 60 kHz demonstrated that the SLAP scheme showed up to 3.5-fold better solvent suppression performance over a traditional solvent-suppression scheme for SSNMR, MISSISSIPPI (Zhou and Rienstra, J Magn Reson 192:167–172, 2008) with 2/3 of the average RF power.

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JEOL Delta pulse programs are available upon request.

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Acknowledgements

This work was in part supported by the JST-Mirai Program (Grant No. JPMJMI17A2, Japan) to Y. I. The instruments employed in the work were partly supported by a JSPS KAKENHI Grant (No. JP15K21772, Japan) to Y. I. RO thanks the Nakatani Foundation for support from its scholarship program. The authors thank the Yokohama RIKEN NMR Facility.

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Authors and Affiliations

  1. RIKEN Center for Biosystems Dynamics Research, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan

    Tatsuya Matsunaga & Yoshitaka Ishii

  2. School of Life Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa, 226-8503, Japan

    Ryotaro Okabe & Yoshitaka Ishii

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  1. Tatsuya Matsunaga
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  2. Ryotaro Okabe
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  3. Yoshitaka Ishii
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Correspondence to Yoshitaka Ishii.

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Matsunaga, T., Okabe, R. & Ishii, Y. Efficient solvent suppression with adiabatic inversion for 1H-detected solid-state NMR. J Biomol NMR 75, 365–370 (2021). https://doi.org/10.1007/s10858-021-00384-8

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