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Expression Levels and Activity of Rat Liver Lactate Dehydrogenase Isoenzymes in Alloxan Diabetes

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Abstract—

A significant decrease in the activity of liver lactate dehydrogenase (LDH, EC 1.1.1.27) associated with a decrease in the expression of the corresponding genes was found in rats with alloxan-induced diabetes. The decrease in the LDH activity was due to the cytoplasmic isoform of this enzyme. It was found that the level of ldha and ldhb gene transcripts in the liver of healthy rats was higher than in animals with alloxan diabetes. The ldha gene expression demonstrated almost 9-fold decrease, while a decrease in the ldhb gene expression was less pronounced (just 1.25-fold). Our data indicate an important role of LDH in the adaptive response of cellular metabolism in the development of type I diabetes mellitus.

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ACKNOWLEDGMENTS

The authors thank Evrogen for synthesis of the oligonucleotides used in the study.

Funding

The study was supported by the Russian Foundation for Basic Research (project no. 20-04-00296).

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Authors and Affiliations

  1. Voronezh State University, Universitetskaya pl. 1, 394006, Voronezh, Russia

    A. T. Eprintsev, I. R. Bondareva & N. V. Selivanova

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  1. A. T. Eprintsev
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  2. I. R. Bondareva
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  3. N. V. Selivanova
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Correspondence to A. T. Eprintsev.

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The authors declare that they have no conflict of interest. All procedures of the experiment complied with the requirements of international rules for the humane treatment of animals, reflected in the sanitary rules for the selection and maintenance of experimental biological clinics (vivaria).

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Translated by A. Medvedev

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Eprintsev, A.T., Bondareva, I.R. & Selivanova, N.V. Expression Levels and Activity of Rat Liver Lactate Dehydrogenase Isoenzymes in Alloxan Diabetes. Biochem. Moscow Suppl. Ser. B 16, 210–215 (2022). https://doi.org/10.1134/S1990750822030052

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  • DOI: https://doi.org/10.1134/S1990750822030052

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