Abstract
Triplophysa hsutschouensis is an endemic species in China, that is mainly distributed in the Hexi Corridor of Gansu Province. Studies on molecular markers in this species are limited. 45 single nucleotide polymorphism markers were developed and characterized in T. hsutschouensis for the first time using restriction site-associated DNA tag sequencing. The minor allele frequency ranged from 0.033 to 0.500, and the observed and expected heterozygosities ranged from 0.067 to 0.600 and 0.066 to 0.508, respectively. The polymorphism information content ranged from 0.062 to 0.375. Among these SNPs, two loci showed significant departures from Hardy–Weinberg equilibrium after Bonferroni correction. The novel polymorphic SNPs will be helpful for further population genetic analysis and natural resource conservation.
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The data and material that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
The authors are grateful to Wenqiong Wu and Siyi Zhu for their assistance with the laboratory work.
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This work was supported by grants from the Project of Investigation on Fishery Resources and Environment of Northwest China, the Project of Sichuan Science and Technology Program (2021YFYZ0015).
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Ya Liu designed the exeriments and wrote the manuscript. Jiansheng Lai, Zhongmeng Zhao, Hongyu Ke and Han Zhao collected specimens. Yeyu Chen provided formal analysis. Xiaoyun Wu performed the experiments. Qiang Li revised the manuscript. All authors read and approved the final manuscript.
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All fish handling and experimental procedures were approved by the Animal Care and Use Committee of the Fishery Institute of the Sichuan Academy of Agricultural Sciences, and all animal collection and use protocols were carried out in accordance with the guidelines and regulations for the care and use of laboratory animals at the Fishery Institute of the Sichuan Academy of Agricultural Sciences.
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Liu, Y., Lai, J., Zhao, Z. et al. Development of 45 SNP markers in Triplophysa hsutschouensis based on RAD sequencing. Conservation Genet Resour 14, 367–369 (2022). https://doi.org/10.1007/s12686-022-01292-1
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DOI: https://doi.org/10.1007/s12686-022-01292-1