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Hepatitis B Virus Genotypes in Patients with Hepatitis D as Determined by the Panel of Their Own Development of Monoclonal Antibodies

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Abstract

Direct genotyping of hepatitis B virus (HBV) in samples from patients with hepatitis delta can be impossible due to an undetectable concentration of HBV DNA. A sufficient amount of surface HBV protein (HBsAg) in such samples makes it possible to determine HBV genotype using enzyme-linked immunosorbent assay (ELISA) of this antigen with a panel of monoclonal antibodies (MABs). The purpose of this paper is to compare the results of HBV genotyping using an in-house MAB panel with the results of molecular analysis of samples from patients with chronic hepatitis B (CHB) and the determination of HBV genotypes in samples from patients with hepatitis delta. A total of 122 serum samples from CHB patients from Yakutia and 211 serum samples from hepatitis delta patients from Yakutia (12 samples) and Tuva (199 samples) were collected. HBV serotypes/genotypes were determined by ELISA using developed reagents. The molecular methods included the isolation of HBV DNA, amplification of the S gene region (713 nt), and phylogenetic analysis of the nucleotide sequences. In a group of samples from CHB patients positive for HBV DNA (86 samples), 95% (82/86) valid results were obtained using our MAB kit. The following genotypes were identified: A, 32 (39%); C, 3 (4%); and D, 47 (57%). The results of HBV genotyping using two methods were identical for 81/82 (99%) samples. HBV genotyping using developed reagents provided 96.2% (203/211) of valid results in samples from patients with hepatitis delta compared to 3.8% of such results obtained using the standard molecular technic (p < 0.001). The following HBV genotypes were identified: A, 17 samples (8.4%), and D, 186 samples (91.6%). The developed test with MAB panel allows one to reliably determine HBV genotype and has advantages over standard molecular methods for HBV genotyping in patients with hepatitis delta.

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Funding

Thos work was financially supported by АО Vector-Best, as well as partially supported by an FSUS-2020-0035 grant for basic research of Novosibirsk State University and the TOP-100 Russian Universities Competitiveness Improvement Program.

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Authors and Affiliations

  1. AO Vector-Best, 630559, Koltsovo, Russia

    L. V. Bezuglova, V. A. Poryvaeva, Ya. D. Mosina, O. A. Agafonova, A. K. Mogilnykh & I. G. Netesova

  2. Russian Medical Academy of Continuous Professional Education, 125993, Moscow, Russia

    O. V. Isaeva, A. A. Karlsen, K. K. Kyuregyan & M. I. Mikhailov

  3. Mechnikov Research Institute for Vaccines and Sera, 105064, Moscow, Russia

    O. V. Isaeva, A. A. Karlsen, L. Y. Ilchenko, K. K. Kyuregyan & M. I. Mikhailov

  4. Pirogov Russian National Research Medical University, 117997, Moscow, Russia

    L. Y. Ilchenko

  5. North Eastern Federal University, Medical Institute, 677000, Yakutsk, Russia

    S. S. Sleptsova

  6. Kyzyl Infectious Hospital, 667005, Kyzyl, Russia

    A. A. Saryglar

  7. Novosibirsk State University, 630090, Novosibirsk, Russia

    S. V. Netesov

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  1. L. V. Bezuglova
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  2. O. V. Isaeva
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  7. V. A. Poryvaeva
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  9. O. A. Agafonova
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Correspondence to L. V. Bezuglova.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Informed consent was obtained from all individual participants involved in the study.

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Bezuglova, L.V., Isaeva, O.V., Karlsen, A.A. et al. Hepatitis B Virus Genotypes in Patients with Hepatitis D as Determined by the Panel of Their Own Development of Monoclonal Antibodies. Mol. Genet. Microbiol. Virol. 37, 91–98 (2022). https://doi.org/10.3103/S0891416822020033

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