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A water-soluble polysaccharide from Eucommia folium: the structural characterization and anti-tumor activity in vivo

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Abstract

In this study, a water-soluble polysaccharide from Eucommia folium was extracted by hot water and purified using Sephadex G-200 gel columns. The results showed that the purified fraction (EFP) has a molecular weight of 9.98 × 105 Da and consisted of rhamnose, arabinose, galactose, glucose, mannose, xylose, galacturonic acid, and glucuronic acid (molar ratio: 0.226: 1.739: 2.183: 1: 0.155: 0.321: 0.358: 0.047). The combination of infrared spectroscopy and NMR analysis proved that EFP is an acidic polysaccharide whose main chain consists of α-L-Araf-(1 → , → 3,5)-α-Araf-(1 → , → 3)-β-Galp-(1 → , → 3,6)-β-Glcp-(1 → , → 2)-α-D-Manp-(1 → , → 4)-α-GalpA-(1 → , → 2,4)-α-Rhap-(1 → . In addition, the in vivo antitumoral activity of EFP was studied using a H22 tumor-bearing mice model. EFP effectively inhibited tumor growth in mice following intragastric administration. By Combining with the results of the apoptosis assay and JC-1 staining analysis, we confirmed that EFP induces apoptosis through the mitochondrial pathway. Furthermore, cell cycle analysis demonstrated that EFP blocks the cell cycle at S phase.

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Data availability statement

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

This research was supported by National Natural Science Foundation of China (No.31801568).

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Authors and Affiliations

  1. State Key Laboratory of Food Nutrition and Safety, Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, College of Food Science and Engineering, Tianjin University of Science &Technology, Tianjin, 300457, People’s Republic of China

    Zhi-Qian Yan, Su-Yun Ding, Pei Chen, Hui-Ping Liu, Meng-Li Chang & Shu-Yuan Shi

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Contributions

Zhi-Qian Yan: Conceptualization, Investigation, Data curation, Writing-Original draft preparation, Writing-Reviewing and Editing. Su-Yun Ding: Methodology. Pei Chen: Software, Formal analysis. Hui-Ping Liu: Resources, Project administration, Funding acquisition. Meng-Li Chang: Software, Validation. Shu-Yuan Shi: supervised.

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Correspondence to Hui-Ping Liu.

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Yan, ZQ., Ding, SY., Chen, P. et al. A water-soluble polysaccharide from Eucommia folium: the structural characterization and anti-tumor activity in vivo. Glycoconj J 39, 759–772 (2022). https://doi.org/10.1007/s10719-022-10086-4

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