Abstract
An efficient and rapid preparative method for the separation and purification of flavonoid glycosides from the Ginkgo biloba extract (GBE) was developed by sephadex LH-20 and preparative high-performance liquid chromatography (HPLC). 40 g GBE of 24% flavonoids were loaded onto the sephadex LH-20 column and five fractions (1.15, 2.57, 1.32, 4.45, and 3.31 g) at flavonoid content of 72.3, 54.2, 63.5, 51.2, and 59.2% were produced. Ultimately, 12 flavonoid glycosides that are at least purities of 97.7% were obtained from 100 mg of each fraction by preparative HPLC. The fraction A, B, and D each contained two flavonoids, yielded 35, 30, 23, 20, 25, and 25 mg, respectively. The fraction C and E each contained three flavonoids, produced 20, 13, 15, 18, 15, and 20 mg, respectively. The chemical structures of the purified compounds were identified by nuclear magnetic resonance (NMR) and electrospray ionization mass spectrometry (ESI/MS).
Funding source: Natural Science Foundation of Liaoning Province
Award Identifier / Grant number: 2021-MS-299
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 31770725
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: This work was supported by the Natural Science Foundation of Liaoning Province (2021-MS-299) and the National Natural Science Foundation of China (31770725).
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Conflict of interest statement: No potential conflict of interest was reported by the authors.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/znc-2022-0124).
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