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Effect of fermentation pH on the structure, rheological properties, and antioxidant activities of exopolysaccharides produced by Alteromonas australica QD

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Abstract

The pH value was essential for the growth and metabolism of microorganisms. Acidic pH exopolysaccharide (AC-EPS) and alkaline pH exopolysaccharide (AL-EPS) secreted by A. australica QD mediated by pH were studied in this paper. The total carbohydrate content and molecular weight of AC-EPS (79.59% ± 2.24% (w/w), 8.374 × 105 Da) and AL-EPS (82.48% ± 1.46% (w/w), 6.182 × 105 Da) were estimated and compared. In AC-EPS, mannose (3.78%) and galactose (3.24%) content was more, while the proportion of glucuronic acid was less in comparison to AL-EPS. The scanning electron microscopy revealed the structural differences among the AC-EPS and AL-EPS. Thermogravimetric analysis showed degradation temperatures of 272.8 °C and 244.9 °C for AC-EPS and AL-EPS, respectively. AC-EPS was found to exhibit better rheological properties and emulsifying capabilities, while AL-EPS had superior antioxidant activities. Overall, both AC-EPS and AL-EPS have the potential to be used as emulsifiers and biological antioxidants.

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Acknowledgements

It is supported by the Open Project Program of Key Laboratory of Ecological Warning, Protection & Restoration for Bohai Sea, Ministry of Natural Resources (2022103), the National Natural Science Funds of China (42106156), the Major Scientific and Technological Innovation Project of Shandong (2021CXGC010705) and the Fundamental Research Funds for the Central Universities (202161007).

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  1. Frontiers Science Center for Deep Ocean Multispheres and Earth System, Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao, 266100, China

    Fengshu Li, Xin Hu, Xiaojun Sun, Haoshuai Li, Yiming Li & Mutai Bao

  2. College of Chemistry & Chemical Engineering, Ocean University of China, Qingdao, 266100, China

    Fengshu Li, Xin Hu, Xiaojun Sun, Haoshuai Li, Jinren Lu, Yiming Li & Mutai Bao

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  6. Yiming Li
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Contributions

Fengshu Li designed and implemented the experiment, and prepared the manuscript. Xin Hu helped to complete the EPS optimization. Xiaojun Sun analyzed antioxidant experimental data. Haoshuai Li analyzed FT-IR data. Jinren Lu helped to complete the rheological experiments. Yiming Li supervised the study. Mutai Bao designed and supervised the study, and completed the final manuscript.

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Correspondence to Mutai Bao.

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Highlights

• A novel exopolysaccharide was isolated from A. australica QD.

• The pH change caused the difference in the structure and properties of AC-EPS and AL-EPS.

• AC-EPS and AL-EPS were structurally composed of Man, GlcN, GlcA, GalN, Glc and Gal.

• AC-EPS and AL-EPS exhibited the potential as emulsifiers and biological antioxidants.

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Li, F., Hu, X., Sun, X. et al. Effect of fermentation pH on the structure, rheological properties, and antioxidant activities of exopolysaccharides produced by Alteromonas australica QD. Glycoconj J 39, 773–787 (2022). https://doi.org/10.1007/s10719-022-10087-3

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  • DOI: https://doi.org/10.1007/s10719-022-10087-3

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