Abstract
The orangeback flying squid, Sthenoteuthis pteropus, is a species of significant potential value that is widely distributed in the tropical and temperate waters of the Atlantic Ocean. There have been no reports of the population genetics and effective molecular markers for this species due to a lack of reliable information regarding its genetic structure and its many individual differences, as well as its complex and changeable life history. Therefore, the development of auxiliary molecular markers would contribute to the development, sustainable utilization, and protection of the species. In this study, 131 novel single nucleotide polymorphism (SNP) markers were developed by double digest restriction-site associated DNA sequencing (ddRAD-Seq). The observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 0.00 to 0.80 and 0.18 to 0.50, respectively. The polymorphism information content (PIC) value ranged from 0.18 to 0.50. None of the marker locations significantly deviated from the Hardy–Weinberg equilibrium (p > 0.05) after a Bonferroni correction. These polymorphic SNPs will be important in the further analysis of the population heredity of S. pteropus and its scientific management.
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Funding
This work was sponsored by the National Key R&D Program of China (2019YFD0901404), the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning under contract 0810000243, the National Nature Science Foundation of China (41876141) and the Shanghai Science and Technology Innovation Action Plan (19DZ1207502).
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YZ carried out all the experiments; CW and HX designed the methods and experiments, interpreted the results and finished the discussion. BL and GL were responsible for overall supervision, and participated in coordination. All authors read and approved the final manuscript.
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Experimental protocols involving live animals were approved by the Ethics Committee for the Use of Animal Subjects of Shanghai Ocean University.
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Zhao, Y., Wang, C., Liu, B. et al. Development and identification of 131 SNP markers in Sthenoteuthis pteropus (Steenstrup). Conservation Genet Resour 14, 371–380 (2022). https://doi.org/10.1007/s12686-022-01293-0
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DOI: https://doi.org/10.1007/s12686-022-01293-0