Abstract
The purpose of the present study was to process and assess the effect of hydrated amnion chorion membrane and dehydrated amnion chorion membrane on proliferation of periodontal ligament (PDL) fibroblast cells. The amnion chorion membrane (ACM) from placenta of 18 systemically healthy patients was obtained from the Department of Obstetrics and Gynaecology. They were processed as hydrated and dehydrated based on different processing methods. The Periodontal ligament cells were obtained from periodontal ligament of freshly extracted premolars of systemically healthy patients, due to orthodontic reasons. The PDL cells were further cultured in laboratory and were exposed to hydrated and dehydrated amnion chorion membrane. The MTT assay was performed to assess the proliferation of PDL fibroblast cells after 24 and 48 h. The hydrated and dehydrated amnion chorion membrane showed proliferation of PDL fibroblasts after 24 and 48 h. The proliferation of PDL fibroblasts in hydrated (p = 0.043) and dehydrated (p = 0.050) amnion chorion membrane was statistically significant at the end of 24 and 48 h respectively. On inter-group comparison dehydrated ACM showed significant proliferation of PDL fibroblasts after 24 (p=0.014) and 48 h (p=0.019). Within the limits of the present study, it can be concluded: both hydrated and dehydrated amnion chorion membrane showed proliferationof PDL fibroblast cells. However, dehydrated ACM showed significant proliferation of PDL fibroblasts.
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References
Barth HD, Launey ME, MacDowell AA, Ager III, J. W., Ritchie RO (2010) On the effect of X-ray irradiation on the deformation and fracture behavior of human cortical bone. Bone 46(6):1475–1485
Chua WK, Oyen ML (2009) Do we know the strength of the chorioamnion?: a critical review and analysis. Eur J Obstet Gynecol Reprod Biol 144:S128–S133
Cooke M, Tan EK, Mandrycky C, He H, O’Connell J, Tseng SCG (2014) Comparison of cryopreserved amniotic membrane and umbilical cord tissue with dehydrated amniotic membrane/chorion tissue. J Wound Care 23(10):465–476
Duan-Arnold Y, Gyurdieva A, Johnson A, Jacobstein DA, Danilkovitch A (2015) Soluble factors released by endogenous viable cells enhance the antioxidant and chemoattractive activities of cryopreserved amniotic membrane. Adv Wound Care 4(6):329–338
Gholipourmalekabadi M, Farhadihosseinabadi B, Faraji M, Nourani MR (2020) How preparation and preservation procedures affect the properties of amniotic membrane? How safe are the procedures? Burns 46(6):1254–1271
Gupta A, Kedige SD, Jain K (2015) Amnion and chorion membranes: potential stem cell reservoir with wide applications in periodontics. Int J Biomater 2015:274082
Kim TG, Ki D, Lee K, So MK, Chung JW, Kang J (2019) Comparison of cytokine expression and ultrastructural alterations in fresh-frozen and dried electron beam-irradiated human amniotic membrane and chorion. Cell Tissue Bank 20(2):163–172
Koizumi N, Inatomi T, Quantock AJ, Fullwood NJ, Dota A, Kinoshita S (2000) Amniotic membrane as a substrate for cultivating limbal corneal epithelial cells for autologous transplantation in rabbits. Cornea 19(1):65–71
Koob TJ, Lim JJ, Massee M, Zabek N, Rennert R, Gurtner G, Li WW (2014) Angiogenic properties of dehydrated human amnion/chorion allografts: therapeutic potential for soft tissue repair and regeneration. Vasc Cell 6(1):1–10
Koob TJ, Lim JJ, Massee M, Zabek N, Denoziere G (2014) Properties of dehydrated human amnion/chorion composite grafts: implications for wound repair and soft tissue regeneration. J Biomed Mater Res Part B Appl Biomater 102(6):1353–1362
Kothiwale SV (2014) The evaluation of chorionic membrane in guided tissue regeneration for periodontal pocket therapy: a clinical and radiographic study. Cell Tissue Bank 15(1):145–152
Marchesan JT, Scanlon CS, Soehren S, Matsuo M, Kapila YL (2011) Implications of cultured periodontal ligament cells for the clinical and experimental setting: a review. Arch Oral Biol 56(10):933–943
McQuilling JP, Vines JB, Mowry KC (2017) In vitro assessment of a novel, hypothermically stored amniotic membrane for use in a chronic wound environment. Int Wound J 14(6):993–1005
Phipps RP, Borrello MA, Blieden TM (1997) Fibroblast heterogeneity in the periodontium and other tissues. J Periodontal Res 32(1):159–165
Rana MP, Mehrotra N (2016) Human amniotic membrane: hope in periodontal regeneration. Int J Sci Res (IJSR) 5(4):564–569
Riau AK, Beuerman RW, Lim LS, Mehta JS (2010) Preservation, sterilization and de-epithelialization of human amniotic membrane for use in ocular surface reconstruction. Biomaterials 31(2):216–225
Schmidt FN, Hahn M, Stockhausen KE, Rolvien T, Schmidt C, Knopp T, Busse B (2022) Influence of X-rays and gamma-rays on the mechanical performance of human bone factoring out intraindividual bone structure and composition indices. Mater Today Bio 13:100169
Thomasen H, Pauklin M, Steuhl KP, Meller D (2009) Comparison of cryopreserved and air-dried human amniotic membrane for ophthalmologic applications. Graefe’s Arch Clin Exp Ophthalmol 247(12):1691–1700
Vahabi S, Vaziri S, Torshabi M, Esfahrood ZR (2015) Effects of plasma rich in growth factors and platelet-rich fibrin on proliferation and viability of human gingival fibroblasts. J Dent (Tehran Iran) 12(7):504
von Versen-Höynck F, Syring C, Bachmann S, Möller DE (2004) The influence of different preservation and sterilisation steps on the histological properties of amnion allografts—light and scanning electron microscopic studies. Cell Tissue Bank 5(1):45–56
Acknowledgement
We would like to thank: a) KLE's Belgaum cancer hospital for sterilization of dehydrated membranes b) KLE’S Dr. Prabhakar Kore’s Basic Science Research Centre (BSRC) for conducting the in-vitro study and c) Skin Bank, KLE’s Dr Prabhakar kore hospital for processing and storage of the samples.
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Khan, A., Kothiwale, S.V. Evaluation on the efficacy of processed hydrated and dehydrated amnion chorion membrane on the proliferation of periodontal ligament fibroblasts. Cell Tissue Bank 25, 349–356 (2024). https://doi.org/10.1007/s10561-023-10077-1
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DOI: https://doi.org/10.1007/s10561-023-10077-1