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Genetic Diversity and Functional Status of the oipA Gene of Helicobacter pylori. Its Association with Genes of the Pathogenicity Island in Patients with Gastroduodenal Diseases in the Republic of Belarus

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Abstract

The aim of this study consisted in an investigation of the variability and functional status of the Helicobacter pylori oipA gene by using a sequencing method and evaluation of the association of oipA functionality with the cagPAI genes and clinical outcomes. The object of the study was 84 samples of H. pylori DNA isolated from antrum biopsies of the gastric mucosa from patients with gastroduodenal diseases. The presence of the cagPAI genes were determined by PCR. The oipA gene status was determined using sequencing reactions. Our results revealed that 79.8% of H. pylori samples had the “on” status of the oipA gene, while the remaining 20.2% of the samples had a nonfunctional gene status. The “on” status of the oipA gene was found to be statistically associated with an increased risk of developing duodenal ulcer compared with superficial gastritis (p < 0.003, OR = 14.5). However, no significant association was observed between the functional oipA status and atrophy gastritis (p < 0.05). The study showed there to be a high frequency of detection of genes of the pathogenicity island among H. pylori DNA samples with an “on” status of the oipA gene. There was a significant correlation between an “on” status of the oipA gene and the presence of all studied cagPAI genes; the highest correlation was found for the cagT and cagM genes. The link between the functional status of the oipA gene and other virulence factors encoded by the pathogenicity island cagPAI is important in the pathogenesis of H. pylori and requires further investigation.

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Authors and Affiliations

  1. The Republican Research and Practical Center for Epidemiology and Microbiology, 220114, Minsk, Belarus

    O. O. Yanovich & L. P. Titov

  2. Medical Center “Nordin”, 220013, Minsk, Belarus

    M. V. Doroshko

Authors
  1. O. O. Yanovich
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  2. L. P. Titov
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  3. M. V. Doroshko
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Correspondence to M. V. Doroshko.

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Statement of Compliance with Standards of Research Involving Humans as Subjects

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

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The authors declare that they have no conflicts of interest.

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Translated by V. Mittova

APPENDIX

APPENDIX

Appendix to the article by O.O. Yanovich, L.P. Titov, and M.V. Doroshko “Genetic Diversity and Functional Status of the oipA Gene of Helicobacter pylori. Its Association with Genes of the Pathogenicity Island in Patients with Gastroduodenal Diseases in the Republic of Belarus.”

Fig. 1.
figure 1

Variations in the nucleotide sequences of the region encoding the signal peptide of the oipA gene. Underline, CT repeats; * stop codon.

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Yanovich, O.O., Titov, L.P. & Doroshko, M.V. Genetic Diversity and Functional Status of the oipA Gene of Helicobacter pylori. Its Association with Genes of the Pathogenicity Island in Patients with Gastroduodenal Diseases in the Republic of Belarus. Mol. Genet. Microbiol. Virol. 37, 173–178 (2022). https://doi.org/10.3103/S0891416822040097

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