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Molecular Typing, Phenotypic and Genotypic Assessment of Antibiotic Resistance and Virulence Factors amongst the Staphylococcus aureus Bacteria Isolated from Raw Chicken Meat

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Abstract

The present research was performed to evaluate the phenotypic and genotypic properties of antibiotic resistance, virulence factor profiles and molecular typing of Staphylococcus aureus strains isolated from chicken meat. A total of 36 S. aureus strains were isolated from 300 raw chicken meat samples. Phenotypic pattern of antibiotic resistance was assessed by disk diffusion. Distribution of antibiotic resistance and virulence genes was evaluated by PCR. Molecular typing of isolates was performed by the ERIC-PCR. Considering the over than 80% similarity, 36 S. aureus isolates were classified in 9 different profiles with 43 to 100% similarities. S. aureus strains showed the highest incidence of resistance against penicillin (100%), tetracycline (91.66%), cephalothin (100%), ciprofloxacin (75%), erythromycin (75%), mupirocin (63.88%), clindamycin (61.11%) and trimethoprim/sulfamethoxazole (61.11%). The most commonly detected antibiotic resistance genes amid the S. aureus isolates were mecA (100%), tetK (80.55%), tetM (66.66%), aacA (6'')/aphD (2'') (61.11%), msrA (55.55%) and ermA (55.55%). Total distribution of etB, etA, tsst-1, clfA and coa virulence factors amongst the S. aureus strains was 61.11, 58.33, 13.88, 75 and 100%, respectively. Genetic cluster of bacteria affected the antibiotic resistance and virulence characters of S. aureus strains. S. aureus strains with the same ERIC-genetic cluster had similar antibiotic resistance and virulence characters which may show their similar origins. Presence of one or more virulence factors and antibiotic resistance genes amongst the resistant S. aureus strains signifies an important public health threat rendering the consumption of raw or undercooked chicken meat.

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ACKNOWLEDGMENTS

The authors would like to thank Dr. Mohammad Zavarshani for his assistance in sample collection. This work was financially supported by the Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.

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  1. Department of Microbiology, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

    Sepideh Karimi & Hassan Momtaz

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The research was extracted from the PhD thesis in the field of Microbiology and was ethically approved by the Council of Research of the Faculty of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran (Consent Ref no. IR.IAU.SHK.REC.1398.051). Verification of this research project and the licenses related to sampling process were approved by the Prof. Hassan Momtaz (Approval Ref no. MIC201946).

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Karimi, S., Momtaz, H. Molecular Typing, Phenotypic and Genotypic Assessment of Antibiotic Resistance and Virulence Factors amongst the Staphylococcus aureus Bacteria Isolated from Raw Chicken Meat. Mol. Genet. Microbiol. Virol. 37, 226–241 (2022). https://doi.org/10.3103/S0891416822040073

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