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Serum total carbohydrates, conjugated carbohydrates and total protein glycation index in diabetes mellitus

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Abstract

Background: Diabetes mellitus is defined according to fasting blood glucose and clinical signs. But, the markers of glycation have been used recently as a criterion to diagnose and monitor the therapy. Objectives: To measure serum total- and conjugated- saccharides and to define the new marker as serum total protein glycation index (sTPGI ) for diabetes. Design and methods: The study population consisted of 172 subjects who were divided to control and diabetic cases. Serum total and conjugated saccharides were measured and sTPGI was defined to discriminate serum glycosylated and glycated saccharides. Results: Patients with diabetes compared with the controls had increased levels of serum (free) glucose, HbA1c, serum total carbohydrates, total conjugated carbohydrates and sTPGI. All three indices of serum carbohydrates showed significant positive correlation with serum glucose, HbA1c and diabetes. The equations: sTPGI = 0.12 Glucose (mg/dL) + 12 and sTPGI = 3.5HbA1c (%) + 5, were deduced for the association of sTPGI with serum free glucose and HbA1c. In ROC analysis, both HbA1c (AUC = 0.965, p ≤ 0.001) and sTPGI (AUC = 0.734, p ≤ 0.001) had strong and significant efficiency to discriminate diabetic cases from control subjects. Conclusions: The results confirm that sTPGI obtained by indirect assay has high significant efficiency comparable to HbA1c to diagnose diabetes. sTPGI relative to HbA1c indicates the mean level of glycaemia over a shorter period of about one month so it responds more quickly to changes in therapy.

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Data Availability

The source of data is available at.

https://isid.research.ac.ir/Mehdi_Rasouli.

Abbreviations

AUC:

area under the curve

AGPs:

advanced glycation products

BUN:

blood urea nitrogen

sTPGI:

Serum total protein glycation index

NBT:

nitroblue tetrazolium

ROC:

receiver operating characteristic curve

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Funding

The project was supported financially by research council of our university.

Author information

Authors and Affiliations

  1. Department of, Clinical Biochemistry, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran

    Sepideh Hosseini & Mehdi Rasouli

  2. Immunogenetics Research Center and Department of Immunology,, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Mazandaran, Iran

    Saeid Abediankenari

Authors
  1. Sepideh Hosseini
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Contributions

Prof Rasouli designed and performed the experiments, analyzed the data, interpreted the findings and wrote the manuscript. Prof Abediankenari is the head of clinical laboratory of Toba, conducted the study and helped to gather the anthropometrics and routine laboratory data of the patients. Hosseini is MSc student and collected the clinical data and performed the main measurements of the study.

Corresponding author

Correspondence to Mehdi Rasouli.

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Statement of ethics

The project was approved by Ethics Committee of Medical Science.

Research at our university [IR.MAZUMS.4.REC.1401.11846].

Study approval statement

This study protocol was reviewed and approved by the committee of department of Biochemistry and Immunogenetic Research Center, approval number [11846].

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The authors declare that they have no conflicts of interest.

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Informed consent was obtained from all individual participants included in the study.

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Hosseini, S., Abediankenari, S. & Rasouli, M. Serum total carbohydrates, conjugated carbohydrates and total protein glycation index in diabetes mellitus. Glycoconj J 40, 375–381 (2023). https://doi.org/10.1007/s10719-023-10115-w

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