Abstract
Xenocypris davidi is an ecologically important fish species that is widely distributed in China. However, the natural genetic diversity of X. davidi has dramatically declined in recent years owing to over-harvesting and water pollution. Data regarding the genetic markers in this species are scarce. In this study, 50 single nucleotide polymorphism (SNP) markers of X. davidi were newly developed using the snapshot method based on transcriptome data and were identified and characterized among three X. davidi populations. For the Huaihe River watershed population, 39 SNPs were polymorphic; for the Yangtze River watershed population, 38 SNPs were polymorphic; and for the Pearl River watershed population, 34 SNPs were polymorphic. Nine, ten, and seven loci significantly deviated from the Hardy–Weinberg equilibrium in the Huaihe River, Yangtze River, and Pearl River populations, respectively. These novel polymorphic SNPs would be useful for further population genetic analyses and natural resource conservation.
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Funding
This work was supported by Key Scientific and Technological Project of Henan Province (202102110263, 212102310850); Xinyang Agriculture and Forestry University Young Teacher Research Fund (2019LG008); the Foundation of Central Laboratory of Xinyang Agriculture and Forestry University (FCL202108).
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Conceptualization: LZ, CS and XG. Methodology and Formal analysis and investigation: CT, CS, JL and HL. Writing—original draft preparation: CS, CT. Writing—review and editing: LZ and XP. Funding acquisition: XG.
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Su, C., Tan, C., Zhao, L. et al. Development and characterization of 50 SNP markers in three watersheds populations of Xenocypris davidi. Conservation Genet Resour 15, 29–47 (2023). https://doi.org/10.1007/s12686-023-01300-y
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DOI: https://doi.org/10.1007/s12686-023-01300-y